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3 results on '"Jing-hao Jin"'

1. Time-Course Transcriptome Profiling Reveals Differential Resistance Responses of Tomato to a Phytotoxic Effector of the Pathogenic Oomycete Phytophthora cactorum

Author

Xue Zhou, Ke Wen, Shen-Xin Huang, Yi Lu, Yang Liu, Jing-Hao Jin, Shiv D. Kale, and Xiao-Ren Chen

Subjects
tomato, transcriptome, Phytophthora cactorum, small cysteine-rich protein, phytotoxicity, defense response, Botany, QK1-989
Abstract

Blight caused by Phytophthora pathogens has a devastating impact on crop production. Phytophthora species secrete an array of effectors, such as Phytophthora cactorum-Fragaria (PcF)/small cysteine-rich (SCR) phytotoxic proteins, to facilitate their infections. Understanding host responses to such proteins is essential to developing next-generation crop resistance. Our previous work identified a small, 8.1 kDa protein, SCR96, as an important virulence factor in Phytophthora cactorum. Host responses to SCR96 remain obscure. Here, we analyzed the effect of SCR96 on the resistance of tomato treated with this recombinant protein purified from yeast cells. A temporal transcriptome analysis of tomato leaves infiltrated with 500 nM SCR96 for 0, 3, 6, and 12 h was performed using RNA-Seq. In total, 36,779 genes, including 2704 novel ones, were detected, of which 32,640 (88.7%) were annotated. As a whole, 5929 non-redundant genes were found to be significantly co-upregulated in SCR96-treated leaves (3, 6, 12 h) compared to the control (0 h). The combination of annotation, enrichment, and clustering analyses showed significant changes in expression beginning at 3 h after treatment in genes associated with defense and metabolism pathways, as well as temporal transcriptional accumulation patterns. Noticeably, the expression levels of resistance-related genes encoding receptor-like kinases/proteins, resistance proteins, mitogen-activated protein kinases (MAPKs), transcription factors, pathogenesis-related proteins, and transport proteins were significantly affected by SCR96. Quantitative reverse transcription PCR (qRT-PCR) validated the transcript changes in the 12 selected genes. Our analysis provides novel information that can help delineate the molecular mechanism and components of plant responses to effectors, which will be useful for the development of resistant crops.

Published
2023
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2. A Novel Transcription Factor CaSBP12 Gene Negatively Regulates the Defense Response against Phytophthora capsici in Pepper (Capsicum annuum L.)

Author

Jing-Hao Jin, Xiao-Hui Feng, Jing-Gang Lv, Su-Yan Gao, Muhammad Ali, Liu-Jun Huang, Huai-Xia Zhang, Abid Khan, Zhen-Hui Gong, and De-Xu Luo

Subjects
0106 biological sciences, 0301 basic medicine, Phytophthora capsici, Nicotiana benthamiana, Acetates, Plant Roots, 01 natural sciences, CaSBP12, lcsh:Chemistry, pepper, Gene Expression Regulation, Plant, Plant defense against herbivory, lcsh:QH301-705.5, Spectroscopy, Disease Resistance, Plant Proteins, biology, food and beverages, General Medicine, Plants, Genetically Modified, defense-related genes, Computer Science Applications, Capsicum, Salicylic Acid, Phytophthora, Transgene, Cyclopentanes, Article, Catalysis, Microbiology, Inorganic Chemistry, 03 medical and health sciences, Pepper, Gene silencing, Gene family, Oxylipins, Physical and Theoretical Chemistry, Molecular Biology, Gene, Plant Diseases, Organic Chemistry, fungi, biology.organism_classification, Plant Leaves, 030104 developmental biology, lcsh:Biology (General), lcsh:QD1-999, Transcription Factors, 010606 plant biology & botany
Abstract

SBP-box (Squamosa-promoter binding protein) genes are a type of plant-specific transcription factor and play important roles in plant growth, signal transduction and stress response. However, little is known about the SBP-box genes in pepper (CaSBP), especially in the process of Phytophthora capsici infection. In this study, a novel gene (CaSBP12) was selected from the CaSBP gene family, which was isolated from the pepper genome database in our previous study. The CaSBP12 gene was located in the nucleus of the cell and its silencing in the pepper plant enhanced the defense response against Phytophthora capsici infection. After inoculation with Phytophthora capsici, the root activity of the CaSBP12-silenced plants is compared to control plants, while malondialdehyde (MDA) content is compared viceversa. Additionally, the expression of defense related genes (CaPO1, CaSAR8.2, CaBPR1, and CaDEF1) in the silenced plants were induced to different degrees and the peak of CaSAR8.2 and CaBPR1 were higher than that of CaDEF1. The CaSBP12 over-expressed Nicotiana benthamiana plants were more susceptible to Phytophthora capsici infection with higher EC (electrical conductivity) and MDA contents as compared to the wild-type. The relative expression of defense related genes (NbDEF, NbNPR1, NbPR1a, and NbPR1b) in transgenic and wild-type Nicotiana benthamiana plants were induced, especially the NbPR1a and NbPR1b. In conclusion, these results indicate that CaSBP12 gene negative regulates the defense response against Phytophthora capsici infection which suggests their potentially significant role in plant defense. To our knowledge, this is the first report on CaSBP gene which negative regulate defense response.

Published
2018

3. The CaAP2/ERF064 Regulates Dual Functions in Pepper: Plant Cell Death and Resistance to Phytophthora capsici

Author

Jing-Hao Jin, Muhammad Ali, De-Xu Luo, Huai-Xia Zhang, Zhen-Hui Gong, and Ai-Min Wei

Subjects
0106 biological sciences, 0301 basic medicine, lcsh:QH426-470, AP2/ERF, Nicotiana benthamiana, 01 natural sciences, Microbiology, 03 medical and health sciences, chemistry.chemical_compound, pepper, Pepper, Genetics, P. capsici, Gene silencing, PR gene, Gene, Genetics (clinical), biology, fungi, food and beverages, biology.organism_classification, lcsh:Genetics, cell death, 030104 developmental biology, Phytophthora capsici, chemistry, Ectopic expression, Phytophthora, 010606 plant biology & botany, Ethephon
Abstract

Phytophthora blight is one of the most destructive diseases of pepper (Capsicum annuum L.) globally. The APETALA2/Ethylene Responsive Factors (AP2/ERF) genes play a crucial role in plant response to biotic stresses but, to date, have not been studied in the context of Phytophthora resistance in pepper. Here, we documented potential roles for the pepper CaAP2/ERF064 gene in inducing cell death and conferring resistance to Phytophthora capsici (P. capsici) infection. Results revealed that the N-terminal, AP2 domain, and C-terminal of CaAP2/ERF064 protein is responsible for triggering cell death in Nicotiana benthamiana (N. benthamiana). Moreover, the transcription of CaAP2/ERF064 in plant is synergistically regulated by the Methyl-Jasmonate (MeJA) and ethephon (ET) signaling pathway. CaAP2/ERF064 was found to regulate the expression of CaBPR1, which is a pathogenesis-related (PR) gene of pepper. Furthermore, the silencing of CaAP2/ERF064 compromised the pepper plant resistance to P. capsici by reducing the transcript level of defense-related genes CaBPR1, CaPO2, and CaSAR82, while the ectopic expression of CaAP2/ERF064 in N. benthamiana plant elevated the expression level of NbPR1b and enhanced resistance to P. capsici. These results suggest that CaAP2/ERF064 could positively regulate the defense response against P. capsici by modulating the transcription of PR genes in the plant.

Published
2019
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