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The identification of flowering plants using DNA barcoding proposed in last decades has slowly gained ground in Africa, where it has been successfully used to elucidate the systematics and ecology of several plant groups, and to understand their evolutionary history. Existing inferences on the effectiveness of DNA barcoding to identify African trees are mostly based on lowland forests, whereas adjacent montane forests significantly differ from the latter floristically and structurally. Here, we tested the efficiency of chloroplast DNA barcodes (rbcLa, matK, and trnH-psbA) to identify Afromontane Forest tree species in a 20.28 ha permanent plot in Ngel Nyaki, Taraba state, Nigeria. We collected, identified, and vouchered 274 individuals with diameter at breast height ≥ 1 cm belonging to 101 morphospecies, 92 genera, and 48 families. rbcLa and matK used alone or in combination performed better than in lowland forests, with the best species discrimination obtained with the two-locus combination of matK + rbcLa. The intragenic spacer trnH-psbA was too variable to align and could not be tested using the genetic distance method employed. Classic DNA barcode can be a powerful tool to identify Afromontane tree species, mainly due to the non-prevalence in these communities of species—rich genera (low species-to-genus ratio) that constitute the biggest challenge of DNA barcoding of flowering plants.

Local factors can play an important role in defining tree species distributions in species rich tropical forests. To what extent the same applies to relatively small, species poor West African montane forests is unknown. Here, forests survive in a grassland matrix and fire has played a key role in their spatial and temporal dynamics since the Miocene. To what extent these dynamics influence local species distributions, as compared with other environmental variables such as altitude and moisture remain unknown. Here, we use data from the 20.28 ha montane forest plot in Ngel Nyaki Forest Reserve, South-East Nigeria to explore these questions. The plot features a gradient from grassland to core forest, with significant edges. Within the plot, we determined tree stand structure and species diversity and identified all trees ≥1 cm in diameter. We recorded species guild (pioneer vs. shade tolerant), seed size, and dispersal mode. We analyzed and identified to what extent species showed a preference for forest edges/grasslands or core forest. Similarly, we looked for associations with elevation, distance to streams and forest versus grassland. We recorded 41,031 individuals belonging to 105 morphospecies in 87 genera and 47 families. Around 40% of all tree species, and 50% of the abundant species, showed a clear preference for either the edge/grassland habitat or the forest core. However, we found no obvious association between species guild, seed size or dispersal mode, and distance to edge, so what leads to this sorting remains unclear. Few species distributions were influenced by distance to streams or altitude.