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TNFAIP1 regulates cellular biological functions, including DNA replication, DNA repair, and cell cycle, by binding to target proteins. Identification of Tnfaip1-interacting proteins contributes to the understanding of the molecular regulatory mechanisms of their biological functions. In this study, 48 hpf, 72 hpf, and 96 hpf wild-type zebrafish embryo mRNAs were used to construct yeast cDNA library. The library titer was 1.12 × 107 CFU/mL, the recombination rate was 100%, and the average length of the inserted fragments was greater than 1000 bp. A total of 43 potential interacting proteins of Tnfaip1 were identified using zebrafish Tnfaip1 as a bait protein. Utilizing GO functional annotation and KEGG signaling pathway analysis, we found that these interacting proteins are mainly involved in translation, protein catabolic process, ribosome assembly, cytoskeleton formation, amino acid metabolism, and PPAR signaling pathway. Further yeast spotting analyses identified four interacting proteins of Tnfaip1, namely, Ubxn7, Tubb4b, Rpl10, and Ybx1. The Tnfaip1-interacting proteins, screened from zebrafish embryo cDNA in this study, increased our understanding of the network of Tnfaip1-interacting proteins during the earliest embryo development and provided a molecular foundation for the future exploration of tnfaip1’s biological functions.

Clear cell renal cell carcinoma (ccRCC) is a highly immunogenic tumor and immune dysfunction is associated with ccRCC poor prognosis. The RhoGTPase-activating proteins (RhoGAPs) family was reported to affect ccRCC development, but its role in immunity and prognosis prediction for ccRCC remain unknown. In the current study, we found ARHGAP11A was the only independent risk factor among 33 RhoGAPs (hazard ratio [HR] 1.949, 95% confidence interval [CI] 1.364–2.785). High ARHGAP11A level was associated with shorter overall survival (OS, HR 2.040, 95% CI 1.646–3.417) and ARHGAP11A is a prognostic biomarker for ccRCC. ARHGAP11A knockdown suppressed renal cell carcinoma (RCC) cell proliferation, colony formation, and migration, suggesting the promoting role of ARHGAP11A on RCC development. Mechanistically, ARHGAP11A might contribute to the suppressive tumor immune microenvironment (TIME). High ARHGAP11A level was correlated with infiltration of immunosuppressive cells (including T helper 2 (Th2) cells, regulatory T (Treg) cells, myeloid derived suppressor cells (MDSC), and M2 macrophage cells), activation of immunosuppressive pathways (IL6-JAK-STAT3 signaling and IFNγ response), and expression of inhibitory immune checkpoints (ICs). ARHGAP11A could promote T cell exhaustion and induce immune escape. ccRCC patients with low ARHGAP11A level were more suitable for immune checkpoint inhibitors (ICIs) therapy, while those with high ARHGAP11A level might benefit from a combination of ARHGAP11A blockade and ICIs. In all, ARHGAP11A might serve as a novel prognostic marker, therapeutic target, and predictor in the clinical response to ICIs therapy for ccRCC.

The longitudinal vibration of the drill pipe contains considerable energy which can be used to improve the rock-breaking efficiency during drilling. It is very important to the development of drilling speed-up tools to have a comprehensive understanding of the energy conversion efficiency of downhole drill string vibration. In this paper, the characteristics of downhole bit load and longitudinal vibration of drill string under different conditions were studied in the experiment, and the analysis method of energy conversion efficiency from drill string vibration to spring potential energy was proposed. The experimental analysis showed that the fluctuation of the downhole bit load was reduced by 10%–90% after the spring was installed in the bottom hole assembly. The rotation rate and the spring elastic stiffness had a significant and positive influence on the fluctuation amplitude of the downhole bit load. Meanwhile, the longitudinal vibration amplitude and acceleration of the drill string peaked at the elastic stiffness of 1 kN/mm. The closer the spring position to the drill bit was, the more severe the longitudinal vibration of the drill string above the spring component was. The bit load and the rotation rate had a positive influence on the severity of longitudinal vibration. The analysis of energy conversion efficiency showed that the available mechanical energy range of the longitudinal vibration of the drill pipe was about 200–420 kW. The work power of the drill string vibration to the spring component increased sharply and then decreased with the increasing of elastic stiffness. The energy conversion efficiency came to the optimal value when the elastic stiffness was between 1 kN/mm and 2 kN/mm. Increasing the rotation rate, keeping the bit load below 134.5 kN and installing the spring component near the drill bit are beneficial for improving the energy conversion efficiency of drill string vibration. This paper reveals the main factors affecting the energy conversion efficiency of drill string vibration and their influencing laws, and determines the range of WOB, rotation speed, spring position and stiffness to obtain the best energy conversion efficiency.