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Acinetobacter baumannii is an important opportunistic pathogen common in clinical infections. Phage therapy become a hot research field worldwide again after the post-antibiotic era. This review summarizes the important progress of phage treatments for A. baumannii in the last five years, and focus on the new interesting advances including the combination of phage and other substances (like photosensitizer), and the phage encapsulation (by microparticle, hydrogel) in delivery. We also discuss the remaining challenges and promising directions for phage-based therapy of A. baumannii infection in the future, and the innovative combination of materials in this area may be one promising direction.

The Yellow River has a high sand content, and sand deposition in the pipelines behind the pumping station occurs from time to time. It is of great significance to reasonably predict the critical velocity of the small-angled V-inclined water transportation pipes. In this study, a Eulerian multiphase model was employed to simulate the solid–liquid two-phase flow. Based on the conservation of the sand transport rate, the critical velocity of the V-inclined pipe was predicted. The effects of simulated pipeline length, pipe inclination and particle size were investigated. The results show that when the simulated pipeline length reached a certain value, it did not affect the prediction of the critical velocity of the overall pipeline. The ±2∘ pipe inclination had a negligible effect on the critical velocity for transporting small-sized particles, but it led to the nonuniform and asymmetrical distribution of liquid velocity and sand deposition at the different cross-sections. As the particle size increased, the critical velocity also increased. However, the influence of particle size on the critical velocity is currently complicated, resulting in a large difference between numerical simulation and empirical formulas when transporting large-sized particles. Accurate prediction of critical velocity is important for long-distance water transportation pipelines to prevent sand deposition and reduce costs.

The Karman vortex street is a common flow phenomenon. In hydraulic machinery, it is usually located downstream of the guide vanes and the runner blades, which reduces hydraulic performance and may also cause fatigue damage to the structure. The latest research suggested that the difference in velocity gradient on each side of the blade trailing edge may have a significant impact on the strength of the wake vortex. The current work aims to verify the above conclusion and further explore the influence of asymmetry of flow velocity on the wake vortex. A numerical model with the velocity ratio, α, between the two sides of the hydrofoil as the only variable was designed, and the wake characteristics were solved by a computational fluid dynamics (CFD) method based on the finite volume. The unsteady Reynolds-average Navier–Stokes (URANS) equations were numerically solved by coupling with a transitional shear-stress transport (SST) turbulence model. The results showed that with the increase of α, the vortex shedding frequency decreased first, and then increased after reaching the critical velocity ratio αc1 ≈ 1.4. The vortex intensity first gradually decreased, and the vortex street suddenly disappeared after reaching the critical velocity ratio αc2 ≈ 2.2. The value of αc1 was affected by the thickness of the trailing edge, and αc2 was affected by the thickness and the Reynolds number. Besides, the asymmetry of the flow velocity also affected the effectiveness of the trailing-edge trimming. This research can provide references for the design of hydraulic machinery and other submerged structures.

Bacillus phage φ29 and its relatives have been considered as one of the most important model organisms for DNA replication, transcription, morphogenesis, DNA packaging studies, and nanotechnology applications. Here, we isolated and characterized a new member of the φ29-like phage, named Bacillus cereus phage vB_BceP-DLc1. This phage, with a unique inserted gene cluster, has the largest genome among known φ29-like phages. DLc1 can use the surface carbohydrate structures of the host cell as receptors and only infects the most related B. cereus strains, showing high host-specificity. The adsorption rate constant and life cycle of DLc1 under experimental conditions were also determined. Not only stable under temperatures below 55 °C and pH range from 5 to 11, the new phage also showed tolerance to high concentrations of NaCl, 75% ethanol, chloroform, and mechanical vortex, which is preferable for practical use in the food and pharmaceutical industries.