1. Transgenic Cotton Plants Expressing the HaHR3 Gene Conferred Enhanced Resistance to Helicoverpa armigera and Improved Cotton Yield
- Author
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Shun Lv, Yehui Xiong, Shupeng Li, Dewen Qiu, Qiang Han, Hongmei Zeng, Yunxin He, Zhigang Zhang, and Zhenzhen Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Transgene ,plant mediated-RNAi ,Genetically modified crops ,RNA interference ,Helicoverpa armigera ,HaHR3 ,transgenic cotton plants ,Moths ,01 natural sciences ,Article ,Catalysis ,Microbiology ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,Gene Expression Regulation, Plant ,Transcription (biology) ,Botany ,Animals ,Bioassay ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Gene ,Spectroscopy ,Disease Resistance ,Plant Diseases ,RNA, Double-Stranded ,Gossypium ,biology ,fungi ,Organic Chemistry ,General Medicine ,Plants, Genetically Modified ,biology.organism_classification ,Computer Science Applications ,RNA silencing ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Insect Proteins ,Transcription Factors ,010606 plant biology & botany - Abstract
RNA interference (RNAi) has been developed as an efficient technology. RNAi insect-resistant transgenic plants expressing double-stranded RNA (dsRNA) that is ingested into insects to silence target genes can affect the viability of these pests or even lead to their death. HaHR3, a molt-regulating transcription factor gene, was previously selected as a target expressed in bacteria and tobacco plants to control Helicoverpa armigera by RNAi technology. In this work, we selected the dsRNA-HaHR3 fragment to silence HaHR3 in cotton bollworm for plant mediated-RNAi research. A total of 19 transgenic cotton lines expressing HaHR3 were successfully cultivated, and seven generated lines were used to perform feeding bioassays. Transgenic cotton plants expressing dsHaHR3 were shown to induce high larval mortality and deformities of pupation and adult eclosion when used to feed the newly hatched larvae, and 3rd and 5th instar larvae of H. armigera. Moreover, HaHR3 transgenic cotton also demonstrated an improved cotton yield when compared with controls.
- Published
- 2017
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