1. On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients
- Author
-
Kuramae, Eiko E., Dimitrov, Mauricio R., da Silva, Gustavo H.R., Lucheta, Adriano R., Mendes, Lucas W., Luz, Ronildson L., Vet, Louise E.M., Fernandes, Tania V., Sub Ecology and Biodiversity, Ecology and Biodiversity, Sub Ecology and Biodiversity, Ecology and Biodiversity, Netherlands Institute of Ecology (NIOO-KNAW), Utrecht University, Universidade Estadual Paulista (Unesp), Microbial Ecology (ME), Terrestrial Ecology (TE), and Aquatic Ecology (AqE)
- Subjects
Microbiology (medical) ,Shotgun metagenomics ,Nitrogen ,Photobioreactor ,010501 environmental sciences ,Chlorella sorokiniana ,01 natural sciences ,Microbiology ,Article ,nitrogen ,03 medical and health sciences ,Virology ,Bioreactor ,phosphorus ,Effluent ,lcsh:QH301-705.5 ,030304 developmental biology ,0105 earth and related environmental sciences ,Blackwater ,0303 health sciences ,Blackwater microbiome ,blackwater microbiome ,Phosphorus ,Pulp and paper industry ,Anaerobic digestion ,Microbial population biology ,Wastewater ,lcsh:Biology (General) ,international ,Environmental science ,Sewage treatment ,Plan_S-Compliant_OA ,shotgun metagenomics - Abstract
Made available in DSpace on 2021-06-25T10:19:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Nederlandse Organisatie voor Wetenschappelijk Onderzoek Wastewater is considered a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed. Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. Our wastewater treatment system also decreased microbial functions related to pathogenesis. In summary, the new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse. Department of Microbial Ecology Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10 Ecology and Biodiversity Institute of Environmental Biology Utrecht University, Padualaan 8 Department of Environmental and Civil Engineering São Paulo State University (UNESP) Department of Terrestrial Ecology Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10 Department of Aquatic Ecology Netherlands Institute of Ecology (NIOO-KNAW), Droevendaalsesteeg 10 Department of Environmental and Civil Engineering São Paulo State University (UNESP) FAPESP: 2013/50351-4 CNPq: 206884/2014-1 Nederlandse Organisatie voor Wetenschappelijk Onderzoek: 729.004.003
- Published
- 2021