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In the present study, 7344 spring observations of a short-distance migratory species, the Eurasian Woodcock, from the Carpathian Basin between 1894 and 1926 were used to investigate the timing of the species’ migration and how different environmental factors influenced it. We used a generalized additive model (GAM) to explore migratory patterns by using environmental and geographical variables. In years when the weather was colder and snowier, the birds migrated weeks later than in years with milder weather. This may be due to the availability of earthworms, which are the most important food for the species. In areas at lower altitudes, migration occurred earlier than in mountainous areas, which may also be due to the different weather. Furthermore, a two week difference was observed between the south-western and north-eastern parts of the Carpathian Basin. This difference is still present nowadays, but the timing of migration has shifted earlier than in the past, probably due to climate change. It would also be important to compare the historical data with recent data to gain a better understanding of the effects of climate change on the migration of the Eurasian Woodcock.

In this paper, we study the initial value problem for the fractional differential equation with multiple deviating arguments. By using Krasnoselskii’s fixed point theorem, the conditions of solvability of the problem are obtained. Furthermore, we establish Ulam–Hyers and generalized Ulam–Hyers stability of the fractional functional differential problem. Finally, two examples are presented to illustrate our results, one is with a pantograph-type equation and the other is numerical.

Background and Objectives: Sarcopenia, a condition characterized by muscle mass loss, is prevalent in up to 68% of rectal cancer patients and has been described as a negative prognostic factor, impacting overall survival and tumor response. While there are extensive data on rectal cancer globally, only a handful of studies have evaluated the role of sarcopenia in locally advanced rectal cancer (LARC). Our study aimed to investigate the relationship between sarcopenia, overall response rate, and toxicity in patients who underwent total neoadjuvant treatment (TNT) for LARC. Materials and Methods: We performed a retrospective study of patients with rectal cancer treated with TNT and surgery with curative intent between 2021 and 2023 at Prof. Dr. Ion Chiricuta Institute of Oncology, Cluj-Napoca. Sarcopenia was assessed on MRI images by measuring the psoas muscle area (PMA) at the level of the L4 vertebra before and after neoadjuvant therapy. The primary endpoints were the overall complete response rate (oCR) and acute toxicity. Results: This study included 50 patients with LARC. The oCR rate was 18% and was significantly associated with post-treatment sarcopenia (OR 0.08, p = 0.043). Patients who did not achieve a clinical or pathologic complete response had, on average, an 8% muscle loss during neoadjuvant therapy (p = 0.022). Cystitis and thrombocytopenia were significantly associated with post-treatment sarcopenia (p = 0.05 and p = 0.049). Conclusions: Sarcopenia and loss of psoas muscle during neoadjuvant therapy were negatively associated with tumor response in locally advanced rectal cancer. Thrombocytopenia and cystitis are more frequent in sarcopenic than non-sarcopenic patients undergoing neoadjuvant chemoradiation for rectal cancer.

Dental caries, a persistent oral health challenge primarily linked to Streptococcus mutans, extends its implications beyond dental decay, affecting over 4 billion individuals globally. Despite its historical association with childhood, dental caries often persists into adulthood with prevalence rates ranging from 60 to 90% in children and 26 to 85% in adults. Currently, there is a dearth of multiepitope vaccines (MEVs) specifically designed to combat S. mutans. To address this gap, we employed an immunoinformatics approach for MEV design, identifying five promising vaccine candidates (PBP2X, PBP2b, MurG, ATP-F, and AGPAT) based on antigenicity and conservation using several tools including CELLO v.2.5, Vaxign, v2.0, ANTIGENpro, and AllerTop v2.0 tools. Subsequent identification of linear B-cell and T-cell epitopes by SVMTrip and NetCTL/NetMHC II tools, respectively, guided the construction of a MEV comprising 10 Cytotoxic T Lymphocyte (CTL) epitopes, 5 Helper T Lymphocyte (HTL) epitopes, and 5 linear B-cell epitopes, interconnected by suitable linkers. The resultant MEV demonstrated high antigenicity, solubility, and structural stability. In silico immune simulations showcased the MEV’s potential to elicit robust humoral and cell-mediated immune responses. Molecular docking studies revealed strong interactions between the MEV construct and Toll-Like Receptors (TLRs) and Major Histocompatibility Complex (MHC) molecules. Remarkably, the MEV–TLR-4 complexes exhibited a low energy score, high binding affinity, and a low dissociation constant. The Molecular Dynamic (MD) simulation analysis suggested that MEV–TLR-4 complexes had the highest stability and minimal conformational changes indicating equilibrium within 40 nanosecond time frames. Comprehensive computational analyses strongly support the potential of the proposed MEV to combat dental caries and associated infections. The study’s computational assays yielded promising results, but further validation through in vitro and in vivo experiments is needed to assess its efficacy and safety.

Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors that sense lipophilic molecules and act as transcription factors to regulate target genes. PPARs have been implicated in the regulation of innate immunity, glucose and lipid metabolism, cell proliferation, wound healing, and fibrotic processes. Some synthetic PPAR ligands are promising molecules for the treatment of inflammatory and fibrotic processes in immune-mediated intestinal diseases. Some of these are currently undergoing or have previously undergone clinical trials. Dietary PPAR ligands and changes in microbiota composition could modulate PPARs’ activation to reduce inflammatory responses in these immune-mediated diseases, based on animal models and clinical trials. This narrative review aims to summarize the role of PPARs in immune-mediated bowel diseases and their potential therapeutic use.

Fiber-based sorbent materials are an essential part of containing oil spills, thus preventing ecological damage. Poly(styrene-b-isobutylene-b-styrene) thermoplastic elastomer fibers were successfully produced by centrifugal spinning. Scanning electron microscopy revealed that the fibers were bead free and smooth-surfaced, with an average fiber diameter of 5.9 ± 2.3 μm. Contact angle measurements proved the highly hydrophobic (water contact angle of 126.8 ± 6.4°) and highly oleophilic nature of the fiber mat. The sorption and retention capacities of the fiber mat were tested for various oils and benchmarked against polypropylene as the industry standard and polystyrene, which is widely used in the literature. The oil uptake of the fiber mat showed a strong correlation with the viscosity of the oil, resulting in sorption capacities of 10.1 ± 0.8 g/g for sunflower oil, 19.9 ± 2.1 g/g for motor oil, and 23.8 ± 1.8 g/g for gear oil. Oil–water separation tests were also conducted, resulting in ~100% oil removal. The thermoplastic elastomer fiber mat outperformed the industry standard; however, the polystyrene fiber mat demonstrated the best oil sorption performance.

Currently, there is a growing demand for ready-to-eat, prepared, high-quality, preservative-free products. However, the shelf-life of these products is often so short that a relatively high percentage of these products cannot be sold and end up as food waste. In this study, pork loin with different marinades (paprika and herbs) was treated with different high hydrostatic pressures (0 MPa, 300 MPa, 450 MPa and 600 MPa) and a bioactive component (piperine) and then the quality of the meat was examined after 0, 4, 8, 12 and 14 days of cold storage. Changes were monitored using color, pH, texture and microbiological analyses. Both pressure, piperine enrichment, storage time and the interactions of different factors had a significant effect on the quality of the loin samples with different marinades. Due to the denaturation of myoglobin, meat slices were less red and lighter after HHP treatment. The addition of piperine reduced this lightness. The pH increased with increasing pressure and decreased with storage time. HHP treatment significantly increased meat hardness, with samples treated at 600 MPa being 19% harder than those treated at 450 MPa. Microbiological results indicated that HHP at 450 MPa and 600 MPa effectively reduced anaerobic total live plate counts, ensuring satisfactory sensory and microbiological quality throughout storage. Piperine fortification also resulted in a more favorable microbiological status during storage without any perceptible change in quality properties during storage. These findings underscore the effectiveness of HHP and piperine enrichment in enhancing the safety and quality of marinated meats.

Background and Objectives: Rectal cancer accounts for approximately one-third of colorectal cancers, with over 340,000 deaths globally in 2022. Despite advancements in treatment, the five-year overall survival for locally advanced rectal cancer (LARC) remains at 74%, with significant morbidity. B7H3 (CD276), an immune checkpoint protein, plays a role in tumor progression and resistance to therapy, and correlates with poor prognosis in various cancers, including colorectal cancer. This study aims to evaluate the expression of B7H3 in LARC and its impact on overall complete response (oCR) rates to neoadjuvant therapy. Methods: A retrospective study was conducted on 60 patients with LARC who received neoadjuvant chemoradiation (nCRT) followed by total mesorectal excision (TME). B7H3 expression was assessed using immunohistochemistry on surgical specimens. Expression levels were categorized as high or low based on a composite score, and their association with oCR rates was analyzed. Results: High B7H3 expression was observed in 60% of patients, with 73.5% showing expression in more than 50% of tumor cells. Patients who achieved oCR had significantly lower B7H3 expression compared to those with residual disease (p < 0.001). No nuclear expression of B7H3 was detected. No significant correlation was found between B7H3 expression and other clinicopathological variables, except for a higher likelihood of non-restorative surgery in patients with elevated B7H3 levels (p = 0.049). Mucinous adenocarcinoma had high expression of B7H3. Conclusions: Elevated B7H3 expression is associated with reduced oCR rates in LARC, highlighting its potential role as a prognostic biomarker. Further studies with larger cohorts are warranted to validate these findings and explore B7H3-targeted therapies as a treatment strategy for LARC.

Durvalumab is an IgG1 monoclonal antibody that has efficacy in many advanced-stage cancers, especially in small-cell lung cancer. The efficacy of durvalumab can be enhanced by chemotherapy. Cutaneous side effects due to treatment with durvalumab are usually self-limiting and easily manageable. We present a clinical case of a female patient aged 61, with small-cell lung carcinoma in stage III B, cT3N2M, who developed a disseminated cutaneous vasculitis after seven months of durvalumab monotherapy, having previously been treated with polychemotherapy according to oncological protocols. To the best of our knowledge, based on a comprehensive search in leading databases, like Web of Science, Scopus, PubMed and some others, ours is the first published case of disseminated cutaneous vasculitis as a result of Durvalumab treatment. Anticancer immunotherapy targeting immune checkpoint inhibition (ICI) has transformed the treatment and evolution of patients with multiple varieties of hematologic cancers. In this context, the cutaneous side effects due to immune checkpoint inhibitor therapies are very few in the scientific literature. Based on this need, we have performed a mini-review of cutaneous side effects due to immune checkpoint inhibitor therapies that treat actual aspects in this sense. We also present some artificial intelligence challenges and future perspectives in the combination of human reasoning and reasoning based on Artificial Intelligence for study of the very rare Disseminate cutaneous vasculitis associated with Durvalumab treatment.

The unusual combination of the fundamentally contradictory properties of high tensile strength and high fracture toughness found in maraging steel makes it well suited for safety-critical applications that require high strength-to-weight materials. In certain instances, additive manufacturing (AM) has produced materials that may be desirable for safety-critical applications where impact toughness is a key property, such as structural parts for the aerospace industry or armor plates for military applications. Understanding the influence of process parameters and defect structure on the properties of maraging steel parts produced via laser powder bed fusion (LPBF) is a fundamental step towards the broader use of AM technologies for more demanding applications. In this research, the impact energy of V-notched specimens made of 1.2709 maraging steel produced by LPBF was determined via Charpy impact testing. Specimens were produced using different processing parameter sets. By combining the process parameters with the porosity values of the parts, we demonstrate that an almost full prediction of the impact properties can be achieved, paving the way for significantly reducing the expenses of destructive testing.

Previous research has primarily focused on evaluating the activity of individual muscles in dancers, often neglecting their synergistic interactions. Investigating the differences in lower limb muscle synergy during landing between dancers and healthy controls will contribute to a comprehensive understanding of their neuromuscular control patterns. This study enrolled 22 Latin dancers and 22 healthy participants, who performed a task involving landing from a 30 cm high platform. The data were collected using Vicon systems, force plates, and electromyography (EMG). The processed EMG data were subjected to non-negative matrix factorization (NNMF) for decomposition, followed by classification using K-means clustering algorithm and Pearson correlation coefficients. Three synergies were extracted for both Latin dancers and healthy participants. Synergy 1 showed increased contributions from the tibialis anterior (p < 0.001) and medial gastrocnemius (p = 0.024) in Latin dancers compared to healthy participants. Synergy 3 highlighted significantly greater contributions from the vastus lateralis in healthy participants compared to Latin dancers (p = 0.039). This study demonstrates that Latin dancers exhibit muscle synergies similar to those observed in healthy controls, revealing specific adjustments in the tibialis anterior and medial gastrocnemius muscles among dancers. This research illustrates how dancers optimize control strategies during landing tasks, offering a novel perspective for comprehensively understanding dancers’ neuromuscular control patterns.

With regard to the heating technology of small test specimens (D < 1 inch, i.e., 25.4 mm), only a limited amount of data and literature are available for making adequate technological decisions. Heating time of small geometric shapes is influenced by the technological parameters of the furnace, the temperature, the disposition technique in the furnace and the geometric characteristics of the workpiece. How to shorten heating time to achieve a suitable material structure is a vital question, while considerable energy is saved at the same time. Among the geometric characteristics, shape dependence is one of the important aspects that must be taken into account in terms of heating technology. Shape dependence is usually taken into account with empirically produced correction factors, which can result in significant oversizing of heating time, energy-wasting technology and material structure of insufficient fineness. In the course of our work, we investigated and compared the shape dependence of cylindrical and prismatic specimens with the same surface-to-volume ratios, which were combined with surface heat transfer analyses and geometric effect tests to formulate new approximate equations for determining heating time. As a result, we could mathematically derive a relationship between heating time, size and shape of the active surfaces, the correlation of which can shorten heating time by 20%. In addition, a shape factor (1.125) between cylinder and prismatic-shaped specimens was determined, which can be used with the new equation to calculate heating time for similar specimens. At last, a relationship is developed between the amount of heat that can be stored in the body during heat equalization and the complexity of the shape, which can be characterized through ratios depending on heating times and active surfaces in the function of total surface/volume ratio. Based on this relationship it can be determined more precisely when heat equalization occurs; therefore, shorter heating time can be achieved. In conclusion, with the help of this new method, optimal heating time for structural steel components, in the case of small cross-section and weight, can be determined.

From 2015 to 2023, we conducted a comprehensive study in the 11,893-hectare hunting area managed by the Marcal-Bitvaközi Hunting Company, characterised by its substantial wild boar population. The research was carried out across various settings, including a free-range wild boar garden during large-scale hunts and free-living areas during individual hunts. We examined 216 wild boars in total, with 173 individuals from free-living areas and 43 from free-range areas. Throughout the sample collection process, we encountered numerous technical challenges that are infrequently detailed in the professional literature, often mentioned only tangentially. This oversight in existing publications neglects the significance of addressing field sampling difficulties, which are crucial for ensuring the precision and accuracy of research. This paper details the equipment requirements, sampling methodologies, and practical solutions to streamline fieldwork. While our primary focus was on endoparasitic infections of the stomach and small intestine, the described methodologies and findings are broadly applicable to research involving all internal organs.

Carcinoembryonic antigen(CEA) is a routine marker employed for follow-up of colorectal tumors. We aimed to determine whether a CEA increase within the normal range can be linked to a risk of recurrence. From the period of 2006–2013 we selected 78 consecutive patients with colorectal cancer, who underwent curative surgery with or without neo-/adjuvant chemo- or radiotherapy and had proper follow-up procedures. For analyzing CEA fluctuation, we used the smallest value of the CEA during follow-up as the reference. With the aid of a Chi-squared test, we have chosen the value of 1.1 ng/mL for significant CEA fluctuation. A total of 43.6% of patients had fluctuations in CEA of at least 1.1 ng/mL, with or without increases above 5 ng/mL. From these, in 79.4% of patients, the increases in CEA were explained either by recurrence (44.1%), adjuvant chemotherapy (20.6%) or benign pathology (14.7%). In 23% of the recurrences, a CEA increase of at least 1.1 ng/mL, but below 5 ng/mL, preceded the clinical relapse by a median of 8 months. Our conclusion is that an increase in CEA levels by at least 1.1 ng/mL within the normal range after curative treatment for colorectal cancer may serve as an early indicator of relapse or could be associated with other pathological conditions.

The aim of this study is to obtain a more complete picture of blood plasma melatonin concentrations in the donkey mares. To this purpose, sampling and statistical processing were carried out in such a way that allowed the researchers to establish the annual and daily rhythms. Based on human observations, according to the hypothesis of the authors, the blood plasma melatonin concentration of pregnant individuals rises during the late gestational period, before parturition. To confirm this, the melatonin concentrations of pregnant and non-pregnant jennies were monitored and compared. In regard to the circannual rhythm, the significantly lowest midnight melatonin value (27.67 pg mL–1) was typical for the summer solstice. Under consideration of circadian changes, a significantly strongest melatonin production (45.16 pg mL–1) was observed on the night of the winter solstice (p < 0.001). Considering gestational age, the blood plasma melatonin concentration (around 38 pg mL–1) does not change as gestation progresses (p = 0.136). The results obtained in this studied population of the domestic ass usefully expand the little knowledge previously gathered about the development of the blood plasma melatonin concentrations of this species.

Rammed earth buildings constitute a large part of the housing stock in rural areas. Although these houses are recognized as a cultural heritage, detailed analyses of their architectural features, geometric parameters crucial for structural stability, and soil properties used for their construction have not yet been carried out in Croatia. The aim of this study is to collect basic data on the architectural features and material properties of rammed earth walls through field research in Croatia. These data are crucial for both numerical and experimental studies to improve the understanding of the structural behavior of rammed earth houses. Data were obtained through field research and a detailed survey of 22 houses. The houses were analyzed, samples of the rammed earth walls were collected, and their properties were tested in the laboratory. This study contributes to a better understanding of regional building practices and provides data that will enable us to identify the causes of damage in future studies and to select rehabilitation measures to preserve the authentic symbols of cultural heritage.

CT angiography might be a suitable procedure to avoid arterial puncture in combined intracavitary and interstitial brachytherapy for cervical cancer curatively treated with combined chemoradiation and brachytherapy boost. Data in the literature about this technique are scarce. We introduced this method and collected brachytherapy data from patients treated in our department between May 2021 and April 2024. We analyzed the applicator subtype, needle insertion (planned versus implanted), implanted depth and the role of CT angiography in selecting needle trajectories and insertion depths. None of the patients managed through this protocol experienced atrial puncture and consequent hemorrhage. Needle positions were accurately selected with the aid of CT angiography with proper coverage of brachytherapy targets and avoidance of organs at risk. CT angiography is a promising method for guiding needle insertion during interstitial brachytherapy.

This study explores the influence of blade design on the low-pressure extrusion process, which is relevant to techniques like spheronization. We investigate how blade geometry affects the extruded paste and final product properties. A model paste was extruded through a basket extruder with varying blade lengths to create distinct wedge gaps (20°, 26° and 32° contact angles). The theoretical analysis explored paste behavior within the gap and extrudate. A model material enabled objective comparison across blade shapes. Our findings reveal a significant impact of blade design on the pressure profile, directly influencing liquid distribution in the paste and extrudate. It also affects the required torque relative to extruder output. The findings of this study hold significant implications for continuous granulation, a technique employed in the pharmaceutical industry for producing granules with uniform size and properties. Understanding the influence of blade geometry on the extrusion process can lead to the development of optimized blade designs that enhance granulation efficiency, improve product quality, and reduce energy consumption. By tailoring blade geometry, manufacturers can achieve more consistent granule characteristics, minimize process variability, and ultimately produce pharmaceuticals with enhanced efficacy.

Introduction: Fluvoxamine is used in children and adolescents (‘youths’) for treating obsessive compulsive disorder (OCD) but also off-label for depressive and anxiety disorders. This study aimed to investigate the relationship between fluvoxamine dose and serum concentrations, independent correlates of fluvoxamine concentrations, and a preliminary therapeutic reference range (TRR) for youths with OCD and treatment response. Methods: Multicenter naturalistic data of a therapeutic drug monitoring service, as well as prospective data of the ‘TDM Vigil study’ (EudraCT 2013-004881-33), were analyzed. Patient and treatment characteristics were assessed by standardized measures, including Clinical Global Impressions—Severity (CGI-S) and —Change (CGI-I), with CGI-I of much or very much improved defining treatment response and adverse drug reactions using the Udvalg for Kliniske Undersogelser (UKU) Side Effect Rating Scale. Multivariable regression analysis was used to evaluate the influence of sex, age, body weight, body mass index (BMI), and fluvoxamine dose on fluvoxamine serum concentrations. Results: The study included 70 youths (age = 6.7–19.6 years, OCD = 78%, mean fluvoxamine dose = 140.4 (range = 25–300) mg/d). A weak positive correlation between daily dose and steady-state trough serum concentrations was found (rs = 0.34, p = 0.004), with dose variation explaining 16.2% of serum concentration variability. Multivariable correlates explaining 25.3% of the variance of fluvoxamine concentrations included higher fluvoxamine dose and lower BMI. Considering responders with OCD, the estimated TRR for youths was 55–371 ng/mL, exceeding the TRR for adults with depression of 60–230 ng/mL. Discussion: These preliminary data contribute to the definition of a TRR in youth with OCD treated with fluvoxamine and identify higher BMI as a moderator of lower fluvoxamine concentrations.

Background: Fabry disease is a progressive, X chromosome-linked lysosomal storage disorder with multiple organ dysfunction. Due to the absence or reduced activity of alpha-galactosidase A (AGAL), glycosphingolipids, primarily globotriaosyl-ceramide (Gb3), concentrate in cells. In heterozygous women, symptomatology is heterogenous and currently routinely used fluorometry-based assays measuring mean activity mostly fail to uncover AGAL dysfunction. The aim was the development of a flow cytometry assay to measure AGAL activity in individual cells. Methods: Conventional and multispectral imaging flow cytometry was used to detect AGAL activity. Specificity was validated using the GLA knockout (KO) Jurkat cell line and AGAL inhibitor 1-deoxygalactonojirimycin. The GLA KO cell line was generated via CRISPR-Cas9-based transfection, validated with exome sequencing, gene expression and substrate accumulation. Results: Flow cytometric detection of specific AGAL activity is feasible with fluorescently labelled Gb3. In the case of Jurkat cells, a substrate concentration of 2.83 nmol/mL and 6 h of incubation are required. Quenching of the aspecific exofacial binding of Gb3 with 20% trypan blue solution is necessary for the specific detection of lysosomal substrate accumulation. Conclusion: A flow cytometry-based assay was developed for the quantitative detection of AGAL activity at the single-cell level, which may contribute to the diagnosis of Fabry patients.

Chlorella vulgaris is of great importance in numerous exploratory or industrial applications (e.g., medicals, food, and feed additives). Rapid quantification of algal biomass is crucial in photobioreactors for the optimization of nutrient management and the estimation of production. The main goal of this study is to provide a simple, rapid, and not-resource-intensive estimation method for determining the algal density of C. vulgaris according to the measured parameters using UV–Vis spectrophotometry. Comparative assessment measurements were conducted with seven different methods (e.g., filtration, evaporation, chlorophyll a extraction, and detection of optical density and fluorescence) to determine algal biomass. By analyzing the entire spectra of diluted algae samples, optimal wavelengths were determined through a stepwise series of linear regression analyses by a novel correlation scanning method, facilitating accurate parameter estimation. Nonlinear formulas for spectrometry-based estimation processes were derived for each parameter. As a result, a general formula for biomass concentration estimation was developed, with recommendations for suitable measuring devices based on algae concentration levels. New values for magnesium content and the average single-cell weight of C. vulgaris were established, in addition to the development of a rapid, semiautomated cell counting method, improving efficiency and accuracy in algae quantification for cultivation and biotechnology applications.

This research investigates the application of plastic fiber reinforcement in pre-tensioned reinforced concrete railway sleepers, conducting an in-depth examination in both experimental and computational aspects. Utilizing 3-point bending tests and the GOM ARAMIS system for Digital Image Correlation, this study meticulously evaluates the structural responses and crack development in conventional and plastic fiber-reinforced sleepers under varying bending moments. Complementing these tests, the investigation employs ABAQUS’ advanced finite element modeling to enhance the analysis, ensuring precise calibration and validation of the numerical models. This dual approach comprehensively explains the mechanical behavior differences and stresses within the examined structures. The incorporation of plastic fibers not only demonstrates a significant improvement in mechanical strength and crack resistance but paves the way for advancements in railway sleeper technology. By shedding light on the enhanced durability and performance of reinforced concrete structures, this study makes a significant contribution to civil engineering materials science, highlighting the potential for innovative material applications in the construction industry.

Payloads for light unmanned free balloons must meet several safety requirements such as being able to protect the inner electronics in order to extract scientific data and to reduce the chance of inflicting personal injury in case of an accidental fall. This article proposes a novel payload structure, which exhibits the form of a dodecahedron. The actual form was determined by carrying out theoretical drop tests on different polyhedrons using the finite element method (FEM). From the simulations, it could be deduced that the dodecahedron was the optimal choice, since the duration of the impact was longer, while the impact force was slightly lower. The payload was produced by additive technologies; therefore, after performing tensile tests on probable materials, PLA was selected as the optimal candidate. The theoretical results about the dodecahedron’s ability were validated by laboratory and real-life drop tests, where the new payload was subjected to 56% less impact force under a 78% longer collision time compared to a classic, rectangular cuboid design. Based on these tests, it was demonstrated that the new structure is safer and it is applicable.

The tumor-to-stroma ratio is a highly debated prognostic factor in the management of several solid tumors and there is no universal agreement on its practicality. In our study, we proposed confirming or dismissing the hypothesis that a simple measurement of stroma quantity is an easy-to-use and strong prognostic tool. We have included 74 consecutive patients with colorectal cancer who underwent primary curative abdominal surgery. The tumors have been grouped into stroma-poor (stroma < 10%), medium-stroma (between 10 and 50%) and stroma-rich (over 50%). The proportion of tumor stroma ranged from 5% to 70% with a median of 25%. Very few, only 6.8% of patients, had stroma-rich tumors, 4% had stroma-poor tumors and 89.2% had tumors with a medium quantity of stroma. The proportion of stroma, at any cut-off, had no statistically significant influence on the disease-specific survival. This can be explained by the low proportion of stroma-rich tumors in our patient group and the inverse correlation between stroma proportion and tumor grade. The real-life proportion of stroma-rich tumors and the complex nature of the stroma–tumor interaction has to be further elucidated.

Proton radiography is a promising development in proton therapy, and researchers are currently exploring optimal detector materials to construct proton radiography detector arrays. High-density glass scintillators may improve integrating-mode proton radiography detectors by increasing spatial resolution and decreasing detector thickness. We evaluated several new scintillators, activated with europium or terbium, with proton response measurements and Monte Carlo simulations, characterizing relative luminosity, ionization quenching, and proton radiograph spatial resolution. We applied a correction based on Birks’s analytical model for ionization quenching. The data demonstrate increased relative luminosity with increased activation element concentration, and higher relative luminosity for samples activated with europium. An increased glass density enables more compact detector geometries and higher spatial resolution. These findings suggest that a tungsten and gadolinium oxide-based glass activated with 4% europium is an ideal scintillator for testing in a full-size proton radiography detector.

The concentration of microplastics (MPs) in two important Hungarian freshwater habitats was determined in the size range of 50 μm–1 mm. Lake Balaton (LB) is the largest shallow lake in Central Europe, with a significant role in recreation and tourism. Its main inflow, the Zala River, enters the lake through an artificially constructed wetland, the Kis-Balaton Water Protection System and its catchment area (KB), which helps preserve the water quality of the lake. From these two areas, 15 samples were taken with an in situ filtration sampling method. After preparation, the samples were analyzed automatically by FT-IR microscopy. All samples, from both areas, contained MPs; the dominant microplastic (MP) shape was the fragment, while the most frequently polymer types were polyethylene, polypropylene and alkyd. Small MPs were dominant in both areas; around 90% of the MPs were smaller than 500 μm. On average, LB contained more MPs (21.0 ± 12.5 MPs/m3) compared to the KB, which presented an average concentration of MPs of 7.8 ± 5.9 MPs/m3. In the examined areas, two potential MP sources were determined, i.e., treated wastewater and road traffic. The importance of tourism should also be further investigated.

Ascaris suum and Macracanthorhynchus hirudinaceus cause a large loss of yield in farm animals as well as in free-living and captive wild boar herds, thereby causing economic damage. This study compared A. suum and M. hirudinaceus infections in free-ranging and captive wild boars (Sus scrofa) in Hungary. The authors measured the A. suum and M. hirudinaceus infections of a 248-hectare wild boar garden and an 11,893-hectare free-living wild boar herd in the sample area. In all cases, samples were collected from shot wild boars. In total, 216 wild boars were examined from June 2015 to June 2023 in Hungary. Of the 173 dissected wild boars from the wild, 57 (32.9%) were infected with A. suum, while 30 (69.8%) of the 43 individuals from the captive area were infected. The prevalence of M. hirudinaceus in the free-living area population was 9.25% (16 wild boars), while that of the captive population was 34.89% (15 wild boars). In the case of the examined helminths, the captive herd was 36.9% more infected than the herd living in the open area.

Amyotrophic lateral sclerosis (ALS) is a fatal form of neuromuscular disease. The aim of this study was to assess changes in the blink reflex (BR) parameters as a valid and easy-to-use tool in ALS patients. We assessed the BR test in patients with a definitive diagnosis of ALS, healthy volunteers, and patients with diseases affecting the peripheral nervous system. The BR was studied in 29 patients who met the Awaji criteria. Latencies were compared with our healthy controls (N = 50) and other diseases of the peripheral nervous system (N = 61). The ALS Functional Rating Scale—Revised (ALSFRS-R) was used to evaluate functional status. Significantly prolonged R2i and R2c latencies were found in the ALS group compared with the healthy control group (p < 0.001). The latencies of R1, R2i, R2c were all increased in the bulbar subtype compared to the limb-onset subtype (p < 0.05). According to our results, BR examination might be a promising tool to monitor the course of the disease or serve as a prognostic biomarker in patients with ALS, but it should be assessed in further studies. The abnormalities detected through BR might help perform earlier interventions in ALS patients and might be useful in other diseases affecting the peripheral nervous system.

The aim of our study was to investigate the effects of a 24-week-long training program on changes in static body posture, as well as the characteristics of anterior shoulder pain in youth swimmers, and the relationship between changes in whole-body posture and the frequency and intensity of anterior shoulder pain. Competitive young swimmers (n = 54, 13.9 ± 1.79 years) were divided into experimental group and control group and both groups performed their usual swimming training. In addition, the experimental group performed a 24-week-long whole-body posture correction program. Before and after the implemented training, whole-body posture was analyzed using the PostureScreen (version 13.7) mobile application, and subjective intensity of pain was determined using the swimmer’s functional pain scale. Significant changes were found between the two groups in numerous measured postural parameters. A significant reduction in the prevalence of shoulder pain and score of the pain scale was observed after the posture correction program in the experimental group. Our results may imply that more optimal biomechanical conditions may indirectly reduce the incidence of swimmer’s shoulder in terms of prevention. Analysis and monitoring of body posture of swimmers using an on-field mobile application continuously, and the application of preventive training programs, may help to avoid developing injuries.

The floods in 2021 in Germany laid bare the need to better integrate volunteers into official disaster management. An online survey with 1767 valid interviews after the floods in July 2021 reveals the shared experiences of professional and non-professional groups. Communication and coordination problems are the main results of the survey analysis. Overall satisfaction was a bit lower for the volunteers than for the professionals. The behavior of the superiors could have been more satisfactory. The preparation and opportunity for reflection could have been better after the operation. The information provision before and during the flood operation could have been more satisfactory. Worries were higher about elderly persons, and misinformation was a perceived danger. Problems experienced in the flood operations ranged from psychological stress to hygiene, self-endangerment, assignment of tasks, and misinformation, both from the media and official sources. Infrastructure-related problems included electricity, water, communication lines, roads, and rail. The suggestions for improvements ranged from communication and information about warnings and behavior to persons that transfer knowledge between organizations, digital exchange platforms, and exchange between countries.

The purpose of this study is to systematically analyze the impact of Industry 4.0 technologies on workforce employability and skills in the South Asian region. The study investigates the driving success factors, challenges, and needed skills by analyzing 48 peer-reviewed articles. The authors searched keywords on the Web of Science database for articles published between 2013 and 2022. The review was conducted using the preferred reporting items for systematic reviews and meta-analyses (PRISMA 2020) and pareto principles. The analysis identifies nine critical success factors, such as artificial intelligence, digital skills, and big data analytics, that contribute to Industry 4.0’s productivity and efficiency. It also identifies six types of challenges, such as training and development, financial constraints, and regulatory issues that must be addressed to grab maximum potential. In addition, the research categorizes five different skills, including the technical, digital, and social skills that are essential for the evolving labor market. The proposed “Industry 4.0 SEI Framework” provides stakeholders with a comprehensive view of the dynamics of Industry 4.0, thereby facilitating policy and industry strategies.

This study investigates Thoroughbred mares exposed or not to supplemental blue light at the end of the gestation. Sixty mares and their 60 foals were selected for the investigation. Guard hair samples were collected from the shoulder just after (within 12 h) the parturition or birth. The foals of the light-treated mares developed significantly (p < 0.05) shorter hair than those of the control mares. A general effect of light treatment on basal hair diameter thinning could be demonstrated (p < 0.005). The maximum force of hair samples of light-treated mares and foals (0.098 and 0.085 N, respectively) was significantly lower than that of the control (0.272 and 0.178 N, respectively). The tensile strength (82.2 N/mm2) of the foal hair samples of the light-treated mares was significantly lower than that of the control foals (121.6 N/mm2). Although no significant difference was found in the elongation (ΔL), the hair of the control animals (mares and foals together) was more elastic than that of the treated animals (335 vs. 262 μm). In conclusion, the supplemental blue light treatment of the pregnant mares has a decreasing effect on both mares and their foals on the mechanical properties of the hair, making it shorter, thinner, and weaker.

The increasing social and environmental challenges, particularly poverty, have brought social entrepreneurship, a highly researched domain, to the attention of academicians. It has emerged as a critical issue in the context of economic development and societal well-being. The current study presents a comprehensive bibliometric analysis in the field of social entrepreneurship and poverty alleviation to explain the current state, geographical performance, and future research agenda. Utilizing VOS viewer (version 1.6.20) and R Studio software (version 4.3.2), 461 final articles were examined and extracted from the Web of Science database, covering the period from 1998 to 2022. The findings reveal a significant increase in research activity in this field since 2009, indicating a growing demand for it as a solution to social challenges. Notably, the years 2021–2022 witnessed a remarkable 55% surge in research output. The Sustainability Journal ranks first as the most productive source, followed by the Journal of Cleaner Production. The most prolific authors are Nina Kolleck from Germany, David Littlewood, and Diane Holt from the UK. Additionally, this study assesses the geographic distribution of research contributions, highlighting regions with relatively lower research performance, such as South Asian and African countries. Leading in this domain are the UK, Spain, the USA, and European institutions. Co-citation patterns reveal four thematic clusters: (1) dynamics of social entrepreneurship; (2) sustainable entrepreneurial ecosystem; (3) social entrepreneurship for social innovation; and (4) integrated sustainable entrepreneurship, shedding light on critical aspects and the intellectual structure of this domain. Finally, keyword co-occurrence analysis identifies emerging research areas, e.g., entrepreneurial development, the role of higher education, enterprise collaboration, inclusive growth, and socio-economic empowerment. This research provides valuable insights for policymakers, researchers, and practitioners committed to achieving sustainable social change.

Liquidity is the ease of converting an asset (physical/digital) into cash or another asset without loss and is shown by the relationship between the time scale and the price scale of an investment. This article examines the illiquidity of Bitcoin (BTC). Bitcoin hash rate information was collected at three different time intervals; parallel to these data, textual information related to these intervals was collected from Twitter for each day. Due to the regression nature of illiquidity prediction, approaches based on recurrent networks were suggested. Seven approaches: ANN, SVM, SANN, LSTM, Simple RNN, GRU, and IndRNN, were tested on these data. To evaluate these approaches, three evaluation methods were used: random split (paper), random split (run) and linear split (run). The research results indicate that the IndRNN approach provided better results.

Active distribution grids that contain energy sources (so-called distributed generation or DG) are nowadays a reality. Besides the many benefits DGs bring to the distribution grid, some challenges are associated with their integration. Since there are DGs now in the distribution grid, the occurrence of islanding operation is possible. Since an islanding operation can be dangerous, it is necessary to have an effective method to detect it. In the last decade, scientists have made a great effort to develop and test various islanding detection methods (IDMs). Many approaches have been tested, and the methods based on computational intelligence (CI) have shown great potential. Among them, artificial neural networks (ANNs) gained most of the research attention. This paper focuses on ANN application for islanding detection. It gives an exhaustive review of the ANN types used for islanding detection, the types of input data, and their transformation to fit the ANNs. Furthermore, various applications based on specific input data, preprocessing types, different learning algorithms, real-time implementation, and various distribution models used for ANN are reviewed. This paper investigates the potential of ANNs to enhance islanding detection accuracy, reduce non-detection zone (NDZ), and contribute to an overall efficient detection method.

Alternative oxidase (Aox) is a terminal oxidase operating in branched electron transport. The activity correlates positively with overflow metabolisms in certain Aspergilli, converting intracellular glucose by the shortest possible path into organic acids, like citrate or itaconate. Aox is nearly ubiquitous in fungi, but aox gene multiplicity is rare. Nevertheless, within the family of the Aspergillaceae and among its various species of industrial relevance—Aspergillus niger, A. oryzae, A. terreus, Penicillium rubens—paralogous aox genes coexist. Paralogous genes generally arise from duplication and are inherited vertically. Here, we provide evidence of four independent duplication events along the lineage that resulted in aox paralogues (aoxB) in contemporary Aspergillus and Penicillium taxa. In some species, three aox genes are co-expressed. The origin of the A. niger paralogue is different than that of the A. terreus paralogue, but all paralogous clades ultimately arise from ubiquitous aoxA parent genes. We found different patterns of uncorrelated gene losses reflected in the Aspergillus pedigree, albeit the original aoxA orthologues persist everywhere and are never replaced. The loss of acquired paralogues co-determines the contemporary aox gene content of individual species. In Aspergillus calidoustus, the two more ancient paralogues have, in effect, been replaced by two aoxB genes of distinct origins.

The high expansion of a variable and intermittent nature of distributed generation, such as photovoltaics (PV), can cause technical issues in existing distribution networks (DN). In addition to producing electrical energy, PVs are inverter-based sources, and can help conventional control mechanisms in mitigating technical issues. This paper proposes a multi-stage optimal power flow (OPF)-based mixed-integer non-linear programming (MINLP) model for improving an operation state in LV PV-rich DN. A conventional control mechanism such as on load tap changer (OLTC) is used in the first stage to mitigate overvoltage caused by PVs. The second stage is related to reducing losses in DN using reactive power capabilities from PVs, which defines the optimization problem as a fully centralized observed from the distribution system operator’s (DSO) point of view. The optimization problem is realized under the co-simulation approach in which the power system analyzer and computational intelligence (CI) optimization method interact through an interface. This approach allows keeping the original MINLP model without approximations and using any computational intelligence method. OpenDSS is used as a power system analyzer, while particle swarm optimization (PSO) is used as a CI optimization method in this paper. Detailed case studies are performed and analyzed over a single-day period. To study validation and feasibility, the proposed model is evaluated on the IEEE LV European distribution feeder. The obtained results suggest that a combination of conventional control mechanisms (OLTC) and inverter-based sources (PVs) represent a promising solution for DSO and can serve as an alternative control method in active distribution networks.

The consumption of artificial and low-calorie sweeteners (ASs, LCSs) is an important component of the Western diet. ASs play a role in the pathogenesis of metabolic syndrome, dysbiosis, inflammatory bowel diseases (IBDs), and various inflammatory conditions. Intestinal nutrient-sensing receptors act as a crosstalk between dietary components, the gut microbiota, and the regulation of immune, endocrinological, and neurological responses. This narrative review aimed to summarize the possible effects of ASs and LCSs on intestinal nutrient-sensing receptors and their related functions. Based on the findings of various studies, long-term AS consumption has effects on the gut microbiota and intestinal nutrient-sensing receptors in modulating incretin hormones, antimicrobial peptides, and cytokine secretion. These effects contribute to the regulation of glucose metabolism, ion transport, gut permeability, and inflammation and modulate the gut–brain, and gut–kidney axes. Based on the conflicting findings of several in vitro, in vivo, and randomized and controlled studies, artificial sweeteners may have a role in the pathogenesis of IBDs, functional bowel diseases, metabolic syndrome, and cancers via the modulation of nutrient-sensing receptors. Further studies are needed to explore the exact mechanisms underlying their effects to decide the risk/benefit ratio of sugar intake reduction via AS and LCS consumption.

Urban green areas serve both the mental and physical health of the people living in the settlements; therefore, the ornamental plants used on green areas currently have a prominent role in reducing the effects of climate change and urbanization, as well as in providing ecosystem services. This is a dynamically changing, new field that requires close cooperation with several scientific fields, such as landscape architecture and plant physiology, genetics, plant breeding, and ecology. The monitoring and research of settlement communities as ecological systems greatly serves the perception of the effects of climate change and helps to mitigate them. The sustainability and economic operation of established urban green space systems can be made effective by applying innovative technologies. The Special Issue “Ornamental Plants and Urban Gardening” was launched in 2022 and published 13 articles on the topic until 31 July 2023. The published articles also have a very wide spectrum of topics, which also shows the diversity and the interdisciplinary nature of the scientific field. In the following, we present the main topics of the published articles and the results with which their authors contributed to the enrichment of the scientific field. We present a brief summary of the articles in shorter subsections.

Although the COVID-19 pandemic is profoundly changing, data on the effect of vaccination and duration of protection against infection and severe disease can still be advantageous, especially for patients with COPD, who are more vulnerable to respiratory infections. The Hungarian COVID-19 registry was retrospectively investigated for risk of infection and hospitalization by time since the last vaccination, and vaccine effectiveness (VE) was calculated in adults with COPD diagnosis and an exact-matched control group during the Delta variant of concern (VOC) wave in Hungary (September–December 2021). For the matching, sex, age, major co-morbidities, vaccination status, and prior infection data were obtained on 23 August 2021. The study population included 373,962 cases divided into COPD patients (age: 66.67 ± 12.66) and a 1:1 matched group (age: 66.73 ± 12.67). In both groups, the female/male ratio was 52.2:47.7, respectively. Among the unvaccinated, there was no difference between groups in risk for infection or hospitalization. Regarding vaccinated cases, in the COPD group, a slightly faster decline in effectiveness was noted for hospitalization prevention, although in both groups, the vaccine lost its significant effect between 215 and 240 days after the last dose of vaccination. Based on a time-stratified multivariate Cox analysis of the vaccinated cases, the hazard was constantly higher in the COPD group, with an HR of 1.09 (95%: 1.05–1.14) for infection and 1.87 (95% CI: 1.59–2.19) for hospitalization. In our study, COPD patients displayed lower vaccine effectiveness against SARS-CoV-2 infection and hospitalization but a similar waning trajectory, as vaccines lost their preventive effect after 215 days. These data emphasize revaccination measures in the COPD patient population.

This study aimed to assess the effectiveness of saline sealing in reducing the incidence of pneumothorax after a CT-guided lung biopsy. This was a retrospective case-control study of patients who underwent CT-guided biopsies for lung tumors using 18 G semiautomatic core needles in conjunction with 17 G coaxial needles. The patients were divided into two consecutive groups: a historical Group A (n = 111), who did not receive saline sealing, and Group B (n = 87), who received saline sealing. In Group B, NaCl 0.9% was injected through the coaxial needle upon its removal. The incidence of pneumothorax and chest tube insertion was compared between the two groups. Multivariate logistic regression was performed to verify the contribution of other pneumothorax risk factors. The study included 198 patients, with 111 in Group A and 87 in Group B. There was a significantly (p = 0.02) higher pneumothorax rate in Group A (35.1%, n = 39) compared to Group B (20.7%, n = 18). The difference regarding chest tube insertion was not significant (p = 0.1), despite a tendency towards more insertions in Group A (5.4%, n = 6), compared to Group B (1.1%, n = 1). Among the risk factors for pneumothorax, only the presence of emphysema (OR = 3.5, p = 0.0007) and belonging to Group A (OR = 2.2, p = 0.02) were significant. Saline sealing of the needle tract after a CT-guided lung biopsy can significantly reduce the incidence of pneumothorax. This technique is safe, readily available, and inexpensive, and should be considered as a routine preventive measure during this procedure.

The paper describes the development of a machine learning-based system to predict the performance of EGNOS. The system is shown to perform considerably better than existing simple geometry-based ‘macro’ models developed for the same purpose in the past.

Even though graphene-family materials (GFMs) hold great promise for various applications, there are still significant knowledge gaps in ecotoxicology and environmental risk assessment associated with their potential environmental impacts. Here, we provide a critical perspective on published ecotoxicity studies of GFMs based on meticulous bibliometric research. Based on the results of our review paper, in order to fill in the current critical knowledge gaps, the following issues are recommended for consideration: performing more studies on GFMs’ effects at environmentally relevant concentrations and more field and laboratory studies with marine and terrestrial organisms. It is also recommended to assess the ecotoxicity of GFMs in more environmentally relevant conditions, such as in trophic chain transfer studies and by multispecies exposure in micro- or mesocosms, as well as gaining insights into the interactive effects between GFMs and environmental pollutants. It was also revealed that despite their widespread detection in different environmental compartments the potential impacts of GFMs in complex test systems where hierarchical trophic organisation or trophic transfer studies are significantly under-represented. One of the main causes was identified as the difficulties in the physicochemical characterisation of GFMs in complex terrestrial test systems or aquatic micro- and mesocosm studies containing a sediment phase. The lack of tools for adequate characterisation of GFMs in these complex test systems may discourage researchers from conducting experiments under environmentally relevant test conditions. In the coming years, fundamental research about these complex test systems will continue to better understand the mechanism behind GFM toxicity affecting organisms in different environmental compartments and to ensure their safe and sustainable use in the future.

Background and Objectives: Progressive supranuclear palsy (PSP) is a neurodegenerative disease, a tauopathy, which results in a wide clinical spectrum of neurological symptoms. The diagnosis is mostly based on clinical signs and neuroimaging; however, possible biomarkers for screening have been under investigation, and the role of the gut microbiome is unknown. The aim of our study was to identify potential blood biomarkers and observe variations in the gut microbiome within a PSP discordant monozygotic twin pair. Materials and Methods: Anthropometric measurements, neuropsychological tests, and the neurological state were evaluated. Blood was collected for metabolic profiling and for the detection of neurodegenerative and vascular biomarkers. Both the gut microbiome and brain MRI results were thoroughly examined. Results: We found a relevant difference between alpha-synuclein levels and moderate difference in the levels of MMP-2, MB, Apo-A1, Apo-CIII, and Apo-H. With respect to the ratios, a small difference was observed for ApoA1/SAA and ApoB/ApoA1. Using a microbiome analysis, we also discovered a relative dysbiosis, and the MRI results revealed midbrain and frontoparietal cortical atrophy along with a reduction in overall brain volumes and an increase in white matter lesions in the affected twin. Conclusions: We observed significant differences between the unaffected and affected twins in some risk factors and blood biomarkers, along with disparities in the gut microbiome. Additionally, we detected abnormalities in brain MRI results and alterations in cognitive functions.

Although radiolabeled alpha-melanocyte stimulating hormone-analogue NAPamide derivatives are valuable melanoma-specific diagnostic probes, their rapid elimination kinetics and high renal uptake may preclude them from being used in clinical settings. We aimed at improving the pharmacokinetics of radiolabeled DOTA-NAPamide compounds by incorporating a 4-(p-iodo-phenyl)-butanoic acid (IPB) into the molecules. Followed by 68Ga-, 205/206Bi-, and 177Lu-labelling, the radiopharmaceuticals ([68Ga]Ga-DOTA-IPB-NAPamide, [205/206Bi]Bi-DOTA-IPB-NAPamide, [177Lu]Lu-DOTA-IPB-NAPamide) were characterized in vitro. To test the imaging behavior of the IPB-containing probes, B16F10 tumor-bearing C57BL/6 mice were subjected to in vivo microPET/microSPECT/CT imaging and ex vivo biodistribution studies. All tracers were stable in vitro, with radiochemical purity exceeding 98%. The use of albumin-binding moiety lengthened the in vivo biological half-life of the IPB-carrying radiopharmaceuticals, resulting in elevated tumor accumulation. Both [68Ga]Ga-DOTA-IPB-NAPamide (5.06 ± 1.08 %ID/g) and [205/206Bi]Bi-DOTA-IPB-NAPamide (4.50 ± 0.98 %ID/g) exhibited higher B16F10 tumor concentrations than their matches without the albumin-binding residue ([68Ga]Ga-DOTA-NAPamide and [205/206Bi]Bi-DOTA-NAPamide: 1.18 ± 0.27 %ID/g and 3.14 ± 0.32; respectively), however; the large amounts of off-target radioactivity do not confirm the benefits of half-life extension for short-lived isotopes. Enhanced [177Lu]Lu-DOTA-IPB-NAPamide tumor uptake even 24 h post-injection proved the advantage of IPB-based prolonged circulation time regarding long-lived radionuclides, although the significant background noise must be addressed in this case as well.