1. The Role of Mitochondria in Sex-Dependent Differences in Hepatic Steatosis and Oxidative Stress in Response to Cafeteria Diet-Induced Obesity in Mice
- Author
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Tiago Yoshida, Juliana Morais Mewes, Paulo Francisco Veiga Bizerra, Byanca Thais Lima de Souza, Fabiana Rodrigues Silva Gasparin, Eduardo Makiyama Klosowski, Rodrigo Polimeni Constantin, Gislaine Cristiane Mantovanelli, Maria Raquel Marçal Natali, Eduardo Hideo Gilglioni, Jorgete Constantin, Mariana Amâncio Daniel da Silva, Emy Luiza Ishii-Iwamoto, Márcio Shigueaki Mito, and Karina Sayuri Utsunomiya
- Subjects
0301 basic medicine ,Mitochondrial ROS ,Male ,medicine.medical_specialty ,Antioxidant ,medicine.medical_treatment ,Mitochondria, Liver ,lcsh:TX341-641 ,Mitochondrion ,medicine.disease_cause ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Oxygen Consumption ,cafeteria diet ,Internal medicine ,medicine ,Animals ,oxidative stress ,Carbon Radioisotopes ,Obesity ,Animal Husbandry ,Beta oxidation ,chemistry.chemical_classification ,Reactive oxygen species ,Nutrition and Dietetics ,Glutathione ,Carbon Dioxide ,medicine.disease ,mitochondrial energy metabolism ,Animal Feed ,Diet ,Fatty Liver ,030104 developmental biology ,Endocrinology ,chemistry ,030220 oncology & carcinogenesis ,Female ,Steatosis ,ER stress ,lcsh:Nutrition. Foods and food supply ,Oxidative stress ,Food Science - Abstract
Female mice fed a cafeteria diet (FCaf) develop higher liver steatosis and oxidative stress than males (MCaf) as a consequence of unresolved ER stress. Here, we investigated whether mitochondria play a role in this sex difference. The isolated mitochondria from FCaf showed more signs of oxidative stress than those of MCaf, correlated with a reduced content of GSH, increased amount of reactive oxygen species (ROS), and lower activities of enzymes involved in ROS neutralisation. Mitochondria from FCaf and MCaf livers exhibited lower rates of succinate-driven state III respiration and reduced ATPase activity in intact coupled mitochondria compared to their controls fed a standard diet (FC and MC), with no differences between the sexes. Fatty acid oxidation in mitochondria and peroxisomes was higher in MCaf and FCaf compared to their respective controls. In the intact perfused liver, there was no difference between sex or diet regarding the fatty acid oxidation rate. These results indicated that cafeteria diet did not affect mitochondrial energy metabolism, even in FCaf livers, which have higher steatosis and cellular oxidative stress. Nevertheless, the increase in mitochondrial ROS generation associated with a decrease in the antioxidant defence capacity, probably contributes to inducing or reinforcing the ER stress in FCaf livers.
- Published
- 2019