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The inter-satellite link is an important technology to improve the accuracy of clock offset measurement and prediction for BeiDou Navigation Satellite System (BDS). At present, BDS measures clock offsets of invisible satellite mainly through the “one-hop” reduction mode based on the satellite-ground clock offset of the node visible satellite and the inter-satellite clock offset between the two satellites. However, there exists a systematic deviation caused by the node satellite reduction, and there is still a large room for improvement in clock offset measurement and prediction. Therefore, this paper firstly proposes a method of whole-network adjustment for clock offset based on the satellite-ground and inter-satellite two-way data. The least square method is used to realize the whole-network adjustment of clock offset based on the observations of two sources, and to obtain optimal estimates of different clock offset reduction. Secondly, the evaluation method combining internal and external symbols are proposed by the fitting residual, prediction error and clock offset closure error. Finally, experimental verification is completed based on BDS measured data. In comparison with the “one-hop” reduction method, the fitting residual and prediction error of the whole-network adjustment method reduces about 45.06% and 52.15%, respectively. In addition, inter-satellite station closure error and three-satellite closure error are reduced from 0.69 ns and 0.23 ns to about 0 ns. It can be seen that the accuracy of BDS time synchronization is significantly improved.

Radio determination satellite service (RDSS) is one of the characteristic services of Beidou navigation satellite system (BDS), and also distinguishes with other GNSS systems. BDS-3 RDSS adopts new signals, which is compatible with BDS-2 RDSS signals in order to guarantee the services of old users. Moreover, the new signals also separate civil signals and military signals which are modulated on different carriers to improve their isolation and RDSS service performance. Timing is an important part of RDSS service, which has been widely used in the field of the power, transportation, marine and others. Therefore, the timing accuracy, availability and continuity is an important guarantee for RDSS service. This paper summarizes the principle of one-way and two-way timing, and provides the evaluation method of RDSS timing accuracy, availability and continuity. Based on BDS-3 RDSS signal measurements of system, the performance of one-way timing and two-way timing is analyzed and evaluated for the first time. The results show that: (1) the accuracy of one-way timing and two-way timing is better than 30 ns and 8 ns respectively, which are better than the official claimed accuracy; (2) the RMS of one-way timing accuracy is 5.45 ns, which is 20% smaller than BDS-2, and the availability and continuity are 100%; (3) the RMS of two-way timing accuracy is 3.59 ns, which is 34% smaller than one-way timing, and both of the availability and continuity are 100%; (4) the orbit maneuver of GEO satellite make the one-way timing has 7.68 h recovery, but has no affection on the two-way timing.

Botulinum neurotoxins (BoNTs) cause the disease called botulism, which can be lethal. BoNTs are proteins secreted by some species of clostridia and are known to cause paralysis by interfering with nerve impulse transmission. Although the human lethal dose of BoNT is not accurately known, it is estimated to be between 0.1 μg to 70 μg, so it is important to enable detection of small amounts of these toxins. Our laboratory previously reported on the development of Endopep-MS, a mass-spectrometric‑based endopeptidase method to detect, differentiate, and quantify BoNT immunoaffinity purified from complex matrices. In this work, we describe the application of Endopep-MS for the analysis of thirteen blinded samples supplied as part of the EQuATox proficiency test. This method successfully identified the presence or absence of BoNT in all thirteen samples and was able to successfully differentiate the serotype of BoNT present in the samples, which included matrices such as buffer, milk, meat extract, and serum. Furthermore, the method yielded quantitative results which had z-scores in the range of −3 to +3 for quantification of BoNT/A containing samples. These results indicate that Endopep-MS is an excellent technique for detection, differentiation, and quantification of BoNT in complex matrices.

Red coleoptiles can help crops to cope with adversity and the key genes that are responsible for this trait have previously been isolated from Triticum aestivum, Triticum urartu, and Aegilops tauschii. This report describes the use of transcriptome analysis to determine the candidate gene that controls the trait for white coleoptiles in T. monococcum by screening three cultivars with white coleoptiles and two with red coleoptiles. Fifteen structural genes and two transcription factors that are involved in anthocyanin biosynthesis were identified from the assembled UniGene database through BLAST analysis and their transcript levels were then compared in white and red coleoptiles. The majority of the structural genes reflected lower transcript levels in the white than in the red coleoptiles, which implied that transcription factors related to anthocyanin biosynthesis could be candidate genes. The transcript levels of MYC transcription factor TmMYC-A1 were not significantly different between the white and red coleoptiles and all of the TmMYC-A1s contained complete functional domains. The deduced amino acid sequence of the MYB transcription factor TmMYB-A1 in red coleoptiles was homologous to TuMYB-A1, TaMYB-A1, TaMYB-B1, and TaMYB-D1, which control coleoptile color in corresponding species and contained the complete R2R3 MYB domain and the transactivation domain. TmMYB-a1 lost its two functional domains in white coleoptiles due to a single nucleotide deletion that caused premature termination at 13 bp after the initiation codon. Therefore, TmMYB-A1 is likely to be the candidate gene for the control of the red coleoptile trait, and its loss-of-function mutation leads to the white phenotype in T. monococcum.

In this study, we prepared cubic-phase oleic-acid-coated KMnF3: Yb3+,Er3+ nanoparticles (NPs) and NaYF4:Yb3+,Er3+ NPs, which were about 23 nm. From the down-shifting emissions spectra of the two NPs obtained by 980 nm excitation, we observed the fact that the KMnF3: 18%Yb3+,1%Er3+ NPs were a luminescent material with a broadband near-infrared emission of 1.5 μm, and full-width at half-maximum (FWHM) of 55 cm−1, which was wider than that of the NaYF4: 18%Yb3+,1% NPs. Therefore, we believe that the oleic-acid-coated KMnF3:Yb3+,Er3+ NPs have great potential in fabricating broadband waveguide amplifiers. Through epitaxial growth of a KMnF3: Yb3+ active-shell on the core NPs, we compounded KMnF3:Yb3+,Er3+@KMnF3:Yb3+ core-active-shell NPs whose 1.5-μm infrared emissions intensity was 3.4 times as strong as that of the core NPs. In addition, we manufactured waveguide amplifiers using KMnF3:18%Yb3+,1%Er3+@KMnF3:2%Yb3+ NPs as the core materials of the waveguide amplifiers. When the input signal power was 0.2 mW and the pump power was 200 mW, we achieved a relative gain of 0.6 dB at 1534 nm in a 10-mm long waveguide.