8 results on '"Cell free supernatant"'
Search Results
2. Correlation between the Antimicrobial Activity and Metabolic Profiles of Cell Free Supernatants and Membrane Vesicles Produced by Lactobacillus reuteri DSM 17938
- Author
-
Alessandro Maccelli, Simone Carradori, Valentina Puca, Francesca Sisto, Paola Lanuti, Maria Elisa Crestoni, Alba Lasalvia, Raffaella Muraro, Helena Bysell, Antonella Di Sotto, Stefan Roos, and Rossella Grande
- Subjects
Lactobacillus reuteri ,Limosilactobacillus reuteri ,cell free supernatant ,metabolomics ,extracellular and membrane vesicles ,antimicrobial activity ,Biology (General) ,QH301-705.5 - Abstract
The aim of the work is to assess the antimicrobial activities of Cell Free Supernatants (CFS) and Membrane Vesicles (MVs), produced by Lactobacillus reuteri DSM 17938, versus Gram-positive and Gram-negative bacteria and investigate their metabolic profiles. The Minimum Inhibitory Concentration was determined through the broth microdilution method and cell proliferation assay while the Minimum Bactericidal Concentration was determined by Colony Forming Units counts. The characteristics of the antimicrobial compounds were evaluated by pH adjustments, proteinase treatment, and size fractionation of the CFS. The cytotoxicity of CFS was tested on two human cell lines. A detailed snapshot of the L. reuteri metabolism was attained through an untargeted metabolic profiling by means of high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) coupled with Electrospray Ionization Source (ESI). The results showed (i) a greater efficacy of CFS and its fractions towards Gram-negative compared to Gram-positive bacteria; (ii) an antimicrobial effect related to pH-dependent compounds but not to MVs; (iii) a molecular weight < 3 KDa as well as an a non-proteinaceous nature of the antimicrobial compounds; and (iv) more than 200 and 500 putative metabolites annotated in MVs and supernatants, covering several classes of metabolites, including amino acids, lipids, fatty and organic acids, polyalcohols, nucleotides, and vitamins. Some putative compounds were proposed not only as characteristic of specific fractions, but also possibly involved in antimicrobial activity.
- Published
- 2020
- Full Text
- View/download PDF
3. Application of Cheese Whey Containing Postbiotics of Lactobacillus acidophilus LA5 and Bifidobacterium animalis BB12 as a Preserving Liquid in High-Moisture Mozzarella.
- Author
-
Sharafi H, Moradi M, and Amiri S
- Abstract
High-moisture mozzarella cheese (HMMC) is a highly perishable cheese with a short shelf life. In this study, the effects of UF cheese whey containing postbiotics from Lactobacillus acidophilus LA-5 (P-LA-5), Bifidobacterium animalis BB-12 (P-BB-12), and their combination on the microbial (i.e., psychrophiles, mesophiles, lactic acid bacteria, and mold-yeast population) and sensory properties of HMMC were investigated. Postbiotics were prepared in a cheese whey model medium as a novel growth media and used as a preserving liquid in HMMC. The results demonstrate that postbiotics reduced the growth of all microorganisms (1.5-2 log CFU/g reduction) and P-LA5 and P-BB12 had the highest antibacterial performance on mesophiles and psychrophiles, respectively. Mold and yeast had the highest susceptibility to the postbiotics. Postbiotics showed a significant effect on maintaining the microbial quality of HMMC during storage, proposing postbiotics as a new preserving liquid for HMMC.
- Published
- 2022
- Full Text
- View/download PDF
4. Bioprospecting Antimicrobials from Lactiplantibacillus plantarum: Key Factors Underlying Its Probiotic Action
- Author
-
Giuseppe Spano, Daniela Fiocco, Pasquale Russo, Maria Teresa Rocchetti, Djamel Drider, and Vittorio Capozzi
- Subjects
Lactiplantibacillus plantarum ,QH301-705.5 ,medicine.drug_class ,Antibiotics ,cell-free supernatant ,Review ,Biology ,Catalysis ,law.invention ,Inorganic Chemistry ,Probiotic ,Anti-Infective Agents ,bacteriocin ,Functional food ,law ,postbiotic ,medicine ,Animals ,Humans ,Biology (General) ,Physical and Theoretical Chemistry ,QD1-999 ,Molecular Biology ,Spectroscopy ,Bioprospecting ,organic acid ,business.industry ,Organic Chemistry ,food and beverages ,General Medicine ,Antimicrobial ,probiosis ,Computer Science Applications ,Biotechnology ,lactic acid bacteria ,Cell free supernatant ,Chemistry ,plantaricin ,Intestinal homeostasis ,Key factors ,probiotics ,Lactobacillaceae ,antiviral extracellular compound ,antibacterial extracellular compound ,business - Abstract
Lactiplantibacillus plantarum (L. plantarum) is a well-studied and versatile species of lactobacilli. It is found in several niches, including human mucosal surfaces, and it is largely employed in the food industry and boasts a millenary tradition of safe use, sharing a long-lasting relationship with humans. L. plantarum is generally recognised as safe and exhibits a strong probiotic character, so that several strains are commercialised as health-promoting supplements and functional food products. For these reasons, L. plantarum represents a valuable model to gain insight into the nature and mechanisms of antimicrobials as key factors underlying the probiotic action of health-promoting microbes. Probiotic antimicrobials can inhibit the growth of pathogens in the gut ensuring the intestinal homeostasis and contributing to the host health. Furthermore, they may be attractive alternatives to conventional antibiotics, holding potential in several biomedical applications. The aim of this review is to investigate the most relevant papers published in the last ten years, bioprospecting the antimicrobial activity of characterised probiotic L. plantarum strains. Specifically, it focuses on the different chemical nature, the action spectra and the mechanisms underlying the bioactivity of their antibacterial and antiviral agents. Emerging trends in postbiotics, some in vivo applications of L. plantarum antimicrobials, including strengths and limitations of their therapeutic potential, are addressed and discussed.
- Published
- 2021
5. Effect of Cell-Free Supernatant from Aeromonas sobria on the Spoilage of Shewanella putrefaciens in Pacific White Shrimp (Litopenaeus vannamei) with the Influence of Temperature Fluctuation
- Author
-
Jing Xie, Yun-Fang Qian, Sheng-Ping Yang, and Jing-Xin Ye
- Subjects
Food spoilage ,Litopenaeus ,temperature fluctuation ,cell-free supernatant ,Shewanella putrefaciens ,Bacterial growth ,lcsh:Technology ,Aeromonas sobria ,lcsh:Chemistry ,03 medical and health sciences ,0404 agricultural biotechnology ,General Materials Science ,Pacific white shrimp ,Food science ,Instrumentation ,lcsh:QH301-705.5 ,Fluid Flow and Transfer Processes ,0303 health sciences ,biology ,030306 microbiology ,Chemistry ,lcsh:T ,Process Chemistry and Technology ,fungi ,General Engineering ,04 agricultural and veterinary sciences ,biology.organism_classification ,040401 food science ,lcsh:QC1-999 ,Computer Science Applications ,Shrimp ,Cell free supernatant ,lcsh:Biology (General) ,lcsh:QD1-999 ,Stationary phase ,lcsh:TA1-2040 ,cold chain logistics ,lcsh:Engineering (General). Civil engineering (General) ,lcsh:Physics ,spoilage potential - Abstract
The objective of this study was to evaluate the effect of cell-free supernatant (CFS) from Aeromonas sobria on the growth and spoilage potential of Shewanella putrefaciens in Pacific white shrimp (Litopenaeus vannamei) during cold chain logistics, including transportation, retailing, and domestic storage. It was shown that the quality of shrimps deteriorated in the cold chain logistics over time. The temperature fluctuation during the experimental period favored the growth of S. putrefaciens, increased the total volatile basic nitrogen (TVB-N) and biogenic amine value, and decreased the sensory quality of shrimps. The application of CFS resulted in the decline on the growth of S. putrefaciens after the early stationary phase stored at a cold condition. It is concluded that the application of CFS can inhibit microbial growth and the spoilage potential of S. putrefaciens and offset the quality deterioration of shrimp exposed to temperature fluctuation during cold chain logistics.
- Published
- 2019
6. Correlation between the Antimicrobial Activity and Metabolic Profiles of Cell Free Supernatants and Membrane Vesicles Produced by Lactobacillus reuteri DSM 17938
- Author
-
Raffaella Muraro, Francesca Sisto, Stefan Roos, Maria Elisa Crestoni, Paola Lanuti, Alessandro Maccelli, Antonella Di Sotto, Helena Bysell, Rossella Grande, Alba Lasalvia, Simone Carradori, and Valentina Puca
- Subjects
Limosilactobacillus reuteri ,0301 basic medicine ,Microbiology (medical) ,Electrospray ionization ,Lactobacillus reuteri ,030106 microbiology ,Microbiology ,biofilm ,03 medical and health sciences ,Minimum inhibitory concentration ,Virology ,lcsh:QH301-705.5 ,antimicrobial activity ,Minimum bactericidal concentration ,biology ,cell free supernatant ,Chemistry ,Broth microdilution ,Metabolism ,Antimicrobial ,biology.organism_classification ,metabolomics ,extracellular and membrane vesicles ,lactobacillus reuteri ,limosilactobacillus reuteri ,probiotics ,Microbiology (Microbiology in the medical area to be 30109) ,030104 developmental biology ,lcsh:Biology (General) ,Biochemistry ,Bacteria - Abstract
The aim of the work is to assess the antimicrobial activities of Cell Free Supernatants (CFS) and Membrane Vesicles (MVs), produced by Lactobacillus reuteri DSM 17938, versus Gram-positive and Gram-negative bacteria and investigate their metabolic profiles. The Minimum Inhibitory Concentration was determined through the broth microdilution method and cell proliferation assay and the Minimum Bactericidal Concentration was determined by Colony Forming Units counts. The characteristics of the antimicrobial compounds were evaluated by pH adjustments, proteinase treatment, and size fractionation of the CFS. The cytotoxicity of CFS was tested on two human cell lines. A detailed snapshot of the L. reuteri metabolism was attained through an untargeted metabolic profiling by means of high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) coupled with Electrospray Ionization Source (ESI). The results showed (i) a greater efficacy of CFS and its fractions towards Gram-negative compared to Gram-positive bacteria, (ii) an antimicrobial effect related to pH-dependent compounds but not to MVs, (iii) a molecular weight <, 3 KDa as well as an a non-proteinaceous nature of the antimicrobial compounds, and (iv) more than 200 and 500 putative metabolites annotated in MVs and supernatants, covering several classes of metabolites, including amino acids, lipids, fatty and organic acids, polyalcohols, nucleotides, and vitamins. Some putative compounds were proposed not only as characteristic of specific fractions, but also possibly involved in antimicrobial activity.
- Published
- 2020
7. Correlation between the Antimicrobial Activity and Metabolic Profiles of Cell Free Supernatants and Membrane Vesicles Produced by Lactobacillus reuteri DSM 17938.
- Author
-
Maccelli A, Carradori S, Puca V, Sisto F, Lanuti P, Crestoni ME, Lasalvia A, Muraro R, Bysell H, Di Sotto A, Roos S, and Grande R
- Abstract
The aim of the work is to assess the antimicrobial activities of Cell Free Supernatants (CFS) and Membrane Vesicles (MVs), produced by Lactobacillus reuteri DSM 17938, versus Gram-positive and Gram-negative bacteria and investigate their metabolic profiles. The Minimum Inhibitory Concentration was determined through the broth microdilution method and cell proliferation assay and the Minimum Bactericidal Concentration was determined by Colony Forming Units counts. The characteristics of the antimicrobial compounds were evaluated by pH adjustments, proteinase treatment, and size fractionation of the CFS. The cytotoxicity of CFS was tested on two human cell lines. A detailed snapshot of the L . reuteri metabolism was attained through an untargeted metabolic profiling by means of high resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR MS) coupled with Electrospray Ionization Source (ESI). The results showed (i) a greater efficacy of CFS and its fractions towards Gram-negative compared to Gram-positive bacteria; (ii) an antimicrobial effect related to pH-dependent compounds but not to MVs; (iii) a molecular weight < 3 KDa as well as an a non-proteinaceous nature of the antimicrobial compounds; and (iv) more than 200 and 500 putative metabolites annotated in MVs and supernatants, covering several classes of metabolites, including amino acids, lipids, fatty and organic acids, polyalcohols, nucleotides, and vitamins. Some putative compounds were proposed not only as characteristic of specific fractions, but also possibly involved in antimicrobial activity., Competing Interests: Authors S.R. and H.B. are currently employed by the company BioGaia AB. Author R.G. has received a research grant from Company BioGaia which supported the microbiological part of the funded project. The remaining authors declare no competing interests.
- Published
- 2020
- Full Text
- View/download PDF
8. Inhibition of Insulin Degrading Enzyme and Insulin Degradation by UV-Killed Lactobacillus acidophilus.
- Author
-
Neyazi N, Motevaseli E, Khorramizadeh MR, Mohammadi Farsani T, Nouri Z, Nasli Esfahani E, and Ghahremani MH
- Abstract
Probiotics have beneficial effects on management of type 2 diabetes (T2D). The major hallmarks of T2D are insulin deficiency and insulin resistance which emphasize insulin therapy in onset of disease. Lactobacilli such as Lactobacillus acidophilus ( L. acidophilus ) have well known properties on prevention of T2D and insulin resistance but not on insulin degradation. Insulin-degrading enzyme (IDE) degrades insulin in the human body. We studied the effects of cell-free supernatant (CFS) and ultraviolet (UV)-killed L. acidophilus (ATCC 314) on IDE activity and insulin degradation in vitro. Cell growth inhibition by CFS and UV-killed L. acidophilus (ATCC 314) was studied and Western blotting and a fluoregenic assay was performed to determine IDE expression and its activity, respectively. Insulin degradation was evaluated by sandwich enzyme-linked immunosorbent assay(ELISA). IDE expression and activity was reduced by CFS and UV-killed L. acidophilus (ATCC 314). Although, decreased enzyme expression and activity was not significant for CFS in contrast to MRL (MRS with same pH as CFS). Also, reduction in IDE activity was not statistically considerable when compared to IDE expression. Insulin degradation was increased by CFS but decreased by UV-killed L. acidophilus (ATCC 314)., Competing Interests: The authors declare no conflict of interest.
- Published
- 2018
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.