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Your search keyword '"Carrion, Ricardo"' showing total 11 results
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11 results on '"Carrion, Ricardo"'

1. Skin Vaccination with Ebola Virus Glycoprotein Using a Polyphosphazene-Based Microneedle Patch Protects Mice against Lethal Challenge

Author

Romanyuk, Andrey, primary, Wang, Ruixue, additional, Marin, Alexander, additional, Janus, Benjamin M., additional, Felner, Eric I., additional, Xia, Dengning, additional, Goez-Gazi, Yenny, additional, Alfson, Kendra J., additional, Yunus, Abdul S., additional, Toth, Eric A., additional, Ofek, Gilad, additional, Carrion, Ricardo, additional, Prausnitz, Mark R., additional, Fuerst, Thomas R., additional, and Andrianov, Alexander K., additional

Published
2022
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2. Single-Shot ChAd3-MARV Vaccine in Modified Formulation Buffer Shows 100% Protection of NHPs

Author

Finch, Courtney L., primary, King, Thomas H., additional, Alfson, Kendra J., additional, Albanese, Katie A., additional, Smith, Julianne N. P., additional, Smock, Paul, additional, Jakubik, Jocelyn, additional, Goez-Gazi, Yenny, additional, Gazi, Michal, additional, Dutton, John W., additional, Clemmons, Elizabeth A., additional, Mattix, Marc E., additional, Carrion, Ricardo, additional, Rudge, Thomas, additional, Ridenour, Alex, additional, Woodin, Sovann F., additional, Hunegnaw, Ruth, additional, Sullivan, Nancy J., additional, and Xu, Rong, additional

Published
2022
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3. Development of a Well-Characterized Cynomolgus Macaque Model of Sudan Virus Disease for Support of Product Development

Author

Alfson, Kendra J., primary, Goez-Gazi, Yenny, additional, Gazi, Michal, additional, Chou, Ying-Liang, additional, Niemuth, Nancy A., additional, Mattix, Marc E., additional, Staples, Hilary, additional, Klaffke, Benjamin, additional, Rodriguez, Gloria F., additional, Escareno, Priscilla, additional, Bartley, Carmen, additional, Ticer, Anysha, additional, Clemmons, Elizabeth A., additional, Dutton III, John W., additional, Griffiths, Anthony, additional, Meister, Gabe T., additional, Sanford, Daniel C., additional, Cirimotich, Chris M., additional, and Carrion, Ricardo, additional

Published
2022
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5. Development of a Well-Characterized Cynomolgus Macaque Model of Marburg Virus Disease for Support of Vaccine and Therapy Development

Author

Alfson, Kendra J., primary, Goez-Gazi, Yenny, additional, Gazi, Michal, additional, Chou, Ying-Liang, additional, Niemuth, Nancy A., additional, Mattix, Marc E., additional, Staples, Hilary M., additional, Klaffke, Benjamin, additional, Rodriguez, Gloria F., additional, Bartley, Carmen, additional, Ticer, Anysha, additional, Clemmons, Elizabeth A., additional, Dutton, John W., additional, Griffiths, Anthony, additional, Meister, Gabe T., additional, Sanford, Daniel C., additional, Cirimotich, Chris M., additional, and Carrion, Ricardo, additional

Published
2022
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6. Development of a Well-Characterized Rhesus Macaque Model of Ebola Virus Disease for Support of Product Development

Author

Alfson, Kendra J., primary, Goez-Gazi, Yenny, additional, Gazi, Michal, additional, Staples, Hilary, additional, Mattix, Marc, additional, Ticer, Anysha, additional, Klaffke, Benjamin, additional, Stanfield, Kaylee, additional, Escareno, Priscilla, additional, Keiser, Patrick, additional, Griffiths, Anthony, additional, Chou, Ying-Liang, additional, Niemuth, Nancy, additional, Meister, Gabe T., additional, Cirimotich, Chris M., additional, and Carrion, Ricardo, additional

Published
2021
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11. Automation of Infectious Focus Assay for Determination of Filovirus Titers and Direct Comparison to Plaque and TCID 50 Assays.

Author

Keiser PT, Anantpadma M, Staples H, Carrion R, and Davey RA

Abstract

Ongoing efforts to develop effective therapies against filoviruses rely, to different extents, on quantifying the amount of viable virus in samples by plaque, TCID 50 , and focus assays. Unfortunately, these techniques have inherent variance, and laboratory-specific preferences make direct comparison of data difficult. Additionally, human errors such as operator errors and subjective bias can further compound the differences in outcomes. To overcome these biases, we developed a computer-based automated image-processing method for a focus assay based on the open-source CellProfiler software platform, which enables high-throughput screening of many treatment samples at one time. We compared virus titers calculated using this platform to plaque and TCID 50 assays using common stocks of virus for 3 major Filovirus species, Zaire ebolavirus , Sudan ebolavirus , and Marburg marburgvirus with each assay performed by multiple operators on multiple days. We show that plaque assays give comparable findings that differ by less than 3-fold. Focus-forming unit (FFU) and TCID 50 assays differ by 10-fold or less from the plaque assays due a higher (FFU) and lower (TCID 50 ) sensitivity. However, reproducibility and accuracy of each assay differs significantly with Neutral Red Agarose Overlay plaque assays and TCID 50 with the lowest reproducibility due to subjective analysis and operator error. Both crystal violet methylcellulose overlay plaque assay and focus assays perform best for accuracy and the focus assay performs best for speed and throughput.

Published
2021
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