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2 results on '"Bossu', M"'

1. Regenerative endodontic procedures using contemporary endodontic materials

Author

Staffoli, S., Plotino, Gianluca, Torrijos, B. G. N., Grande, Nicola Maria, Bossu, M., Gambarini, G., Polimeni, A., Plotino G., Grande N. M., Staffoli, S., Plotino, Gianluca, Torrijos, B. G. N., Grande, Nicola Maria, Bossu, M., Gambarini, G., Polimeni, A., Plotino G., and Grande N. M.

Abstract

Calcium hydroxide apexification and Mineral Trioxide Aggregate (MTA) apexification are classical treatments for necrotic immature permanent teeth. The first tend to fail for lack of compliance given the high number of sessions needed; the second has technical difficulties such as material manipulation and overfilling. With both techniques, the root development is interrupted leaving the tooth with a fragile root structure, a poor crown-to-root ratio, periodontal breakdown, and high risk of fracture, compromising long-term prognosis of the tooth. New scientific literature has described a procedure that allows complete root development of these specific teeth. This regenerative endodontic procedure (REP) proposes the use of a combination of antimicrobials and irrigants, no canal walls instrumentation, induced apical bleeding to form a blood clot and a tight seal into the root canal to promote healing. MTA is the most used material to perform this seal, but updated guidelines advise the use of other bioactive endodontic cements that incorporate calcium and silicate in their compositions. They share most of their characteristics with MTA but claim to have fewer drawbacks with regards to manipulation and aesthetics. The purpose of the present article is to review pertinent literature and to describe the clinical procedures protocol with its variations, and their clinical application.

Published
2019

2. Biocompatibility and Antibiofilm Properties of Calcium Silicate-Based Cements: An In Vitro Evaluation and Report of Two Clinical Cases

Author

Marco Rulli, Adele Preziosi, Antonella Polimeni, Erika Bruni, Maurizio Bossù, Michela Relucenti, Orlando Donfrancesco, Alessandro Salucci, Patrizia Mancini, Gianni Di Giorgio, Daniela Uccelletti, Flavia Iaculli, Roberto Matassa, Bossu, M., Mancini, P., Bruni, E., Uccelletti, D., Preziosi, A., Rulli, M., Relucenti, M., Donfrancesco, O., Iaculli, F., Di Giorgio, G., Matassa, R., Salucci, A., and Polimeni, A.

Subjects
Mineral trioxide aggregate, medicine.medical_specialty, Biocompatibility, QH301-705.5, antibiofilm properties, bioactive cements, biocompatibility, biodentine, mineral trioxide aggregate, 02 engineering and technology, Article, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, In vivo, medicine, Antibiofilm propertie, Viability assay, Biology (General), Cytotoxicity, General Immunology and Microbiology, biology, 030206 dentistry, 021001 nanoscience & nanotechnology, Endodontics, biology.organism_classification, Streptococcus mutans, chemistry, Bioactive cement, Calcium silicate, 0210 nano-technology, General Agricultural and Biological Sciences, Biomedical engineering
Abstract

Simple Summary Calcium silicate-based cements are successfully applied in the different fields of endodontics and vital pulp therapy. To better assess the properties of these bioactive materials, the present in vitro and in vivo study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured in the presence of both materials and evaluated. Moreover, the bioactive cements were in vivo applied to perform vital pulp therapy on immature permanent teeth affected by reversible pulpitis. Saos-2 cells’ viability was slightly greater in the presence of ProRootMTA than Biodentine and cells would grow in a better way on ProRootMTA disks than on Biodentine ones. Moreover, ProRootMTA showed a powerful antibiofilm effect towards Streptococcus mutans. The in vitro results were clinically supported by a 100% success rate after 2 years of follow-up. Abstract Calcium silicate-based cements have reached excellent levels of performance in endodontics, providing predictable and successful results. To better assess the properties of these bioactive materials, the present study aimed to compare the biocompatibility and antibiofilm properties of ProRoot MTA and Biodentine. Human osteogenic sarcoma (Saos-2) cells were cultured on ProRoot MTA and Biodentine samples or in the presence of both cement extracts. Cell viability assay, measurement of reactive oxygen species (ROS), immunofluorescence analysis, as well as morphological evaluations were conducted. Moreover, Streptococcus mutans was used to assess the biofilm forming ability on ProRoot MTA and Biodentine disks. Finally, both cements were applied in vivo to treat immature permanent teeth affected by reversible pulpitis. Results: Cell viability assay demonstrated that Saos-2 cells had a dose- and time-dependent cytotoxicity to both analyzed cements, although cells exposed to ProRoot MTA showed a better cell vitality than those exposed to Biodentine (p < 0.001). Both cements demonstrated ROS production while this was greater in the case of Biodentine than ProRoot MTA (p < 0.001). Immunofluorescence images of the cytoskeleton and focal adhesions showed no differences in Saos-2 cells grown in the presence of ProRoot MTA eluate; whereas in the Biodentine groups, cells showed a morphology and focal adhesions more similar to that of the control sample, as the eluate concentration decreased. Morphological analysis revealed that Saos-2 cells were more flattened and exhibited better spreading when attached to ProRoot MTA disks than to Biodentine ones. The antibiofilm properties showed a time-dependent powerful inhibition of S. mutans superficial colonization and an antibiofilm effect of both cements. Clinically, complete root formation of the treated elements was achieved using the two studied cements, showing stable results over time. ProRoot MTA and Biodentine was demonstrated to be biocompatible and to possess antibiofilm properties. Their clinical application in vital pulp therapy provided successful outcomes after 2 years of follow-up.

Published
2021
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