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Starch extracted from ten lotus root varieties (Yeyong, MRHZ, Zhonghua, Suiningbaiou, Zaohua, Meirenhong, E9:11, Huchengyeou, Peixianyeou, Xin No. 5) from Jiangsu Province, China, were studied. The structural characteristics of lotus root starch were analyzed based on its physical and chemical properties, particle structure, crystal structure, layered structure, thermal properties and gel properties. The lotus root starches were generally milky white, with a starch content ranging from 81.72 to 99.54%, amylose content ranging from 15.44 to 23.54%, light transmittance between 15.30 and 26.90%, and swelling power and solubility at 22.15–29.72% and 2.00–6.00%, respectively. Among the varieties, “Suiningbaiou” had the whitest starch, while “Meirenhong” and “Suiningbaiou” had higher starch contents. “Huchengyeou” had the lowest amylose content, and “Zhonghua” showed the highest light transmittance. “Yeyong” and “Zaohua” had higher solubility, whereas “Zhonghua” had the highest swelling power. An optical, polarizing and scanning electron microscope revealed that most of the lotus starch was oval. However, starch from “Zhonghua”, “E9:11”, “Peixianyeou” and “Zaohua” was longer, whereas starch from “Meirenhong”, “MRHZ”, “Huchengyeou”, “Yeyong”, “Suiningbaiou” and “Xin No.5” was shorter. The X-ray powder diffraction patterns and 13C NMR spectroscopy indicated that “Zaohua” starch exhibited type A crystallization, while starch from the other varieties displayed type CA crystallization, with a relative crystallinity between 18.27 and 22.10%; “Huchengyeou” and “Yeyong” had higher crystallinity. Infrared spectroscopy and small-angle X diffraction analysis showed similar results for short-range order and lamella spacing. The thermal and gel properties of starch from the ten lotus root varieties also varied. The enthalpy change values of “Xin No.5” and “Yeyong” were higher, and starch from “Yeyong” and “Huchengyeou” showed a greater gel strength. Finally, factor score analysis ranked the starch quality of the top four lotus root varieties in Jiangsu as “Suiningbaiou”, “Yeyong”, “Huchengyeou”, and “Zaohua”.

Micro-corona devices could be extensively utilized in gas sensing, switchgear, biomedicine, and other fields. As the influence mechanism of the cathode curvature radius on micro-corona discharge dynamical processes is very important for performance optimization and the promotion of these devices, a micro-scale corona discharge gas model in a mixture of N2-O2 is proposed based on the fluid–chemical mixing method, which describes the dynamic process of the discharge at atmosphere and normal temperatures. To reveal the influence mechanism of the nanowire curvature radius on the micro-corona discharge, the effect of the cathode nanowire radius on the discharge current, electric field, ionization reaction rate, and charged particle characteristics at different gaps and voltages were determined. The findings indicate that the effect of curvature radius on discharge intensity varies under different gap and voltage conditions. Further analysis indicates that an increase in curvature radius reduces the electric field near the tip while increasing the ionization area and secondary emission area as well as the number of positive ions in the space, consequently affecting the coupling process between the collision ionization and the secondary emission. Especially under the conditions of either small gap or low voltage, a suitable increase in the curvature radius could promote the coupling process and then increase the discharge current.

Arterial stiffness has been proved to be an important parameter in the evaluation of cardiovascular diseases, and Pulse Wave Velocity (PWV) is a strong indicator of arterial stiffness. Compared to regional PWV (PWV among different arteries), local PWV (PWV within a single artery) outstands in providing higher precision in indicating arterial properties, as regional PWVs are highly affected by multiple parameters, e.g., variations in blood vessel lengths due to individual differences, and multiple reflection effects on the pulse waveform. However, local PWV is less-developed due to its high dependency on the temporal resolution in synchronized signals with usually low signal-to-noise ratios. This paper presents a method for the noninvasive simultaneous measurement of two local PWVs in both left and right radial arteries based on the Fiber Bragg Grating (FBG) technique via correlation analysis of the pulse pairs at the fossa cubitalis and at the wrist. Based on the measurements of five male volunteers at the ages of 19 to 21 years old, the average left radial PWV ranged from 9.44 m/s to 12.35 m/s and the average right radial PWV ranged from 11.50 m/s to 14.83 m/s. What is worth mentioning is that a stable difference between the left and right radial PWVs was observed for each volunteer, ranging from 2.27 m/s to 3.04 m/s. This method enables the dynamic analysis of local PWVs and analysis of their features among different arteries, which will benefit the diagnosis of early-stage arterial stiffening and may bring more insights into the diagnosis of cardiovascular diseases.

The global infectious disease COVID-19 is caused by SARS-CoV-2, a new member of the Coronaviridae family. Though presented as a novel disease that primarily affects the respiratory system, multi-organ involvement has been well-noticed and documented since the beginning of the pandemic. When performed properly with adequate safety measures, autopsy provides the most valuable information to decipher the pathogenesis of this novel disease, therefore providing a basis for clinical management. In addition to reviewing the macroscopic changes in organs and tissues involved in COVID-19, the relevant microscopic alterations and possible pathogenesis are also discussed.

Over prolonged exposure to groundwater conditions, semi-rigid base asphalt pavements can undergo significant changes in their internal moisture field, resulting in substantial variations in the pavement’s stiffness and, consequently, affecting the overall load-bearing capacity and stability of the road structure. This paper employs FWD non-destructive testing equipment to assess its mechanical performance and conduct data analysis and conducts a mechanical response study of asphalt road surfaces, considering the influence of roadbed moisture levels. Using the dynamic analytical theory, the fundamental equations and stiffness matrices for a linear elastic half-space model were established, leading to the development of a computational model for the mechanical response of semi-rigid base asphalt pavements under FWD dynamic loading, with an examination of the surface deflection in relation to changes in groundwater levels. Numerical examples and engineering applications were employed to validate the proposed model. The research findings indicate: With the passage of time, surface deflection values initially increase and then decrease, exhibiting a sinusoidal variation pattern similar to that of the applied load. As the distance from the loading center increases, the moment of peak deflection continually lags behind. The average absolute relative error between the results obtained using the method proposed in this study and the traditional ABAQUS finite element method was only 0.70%. The correlation coefficient between the theoretically computed deflection curve and the measured deflection curve using the spectral element method was greater than 0.9, with an average absolute relative error of 4.92% between the theoretical peak deflection and the measured peak deflection. As the groundwater level rises, surface deflection noticeably increases, with an approximately 40% increase in deflection values at the loading center. These research findings can be utilized to analyze the dynamic deflection of semi-rigid base asphalt pavements under various groundwater conditions, providing significant practical value for assessing road structural performance and serviceability.

A quasi-static finite cavity cylindrical expansion model is proposed to investigate the lateral boundary effect during long-rod penetration. Analytical solutions for the cavity pressure in the elastic–plastic stage and plastic stage are obtained, through which a decay function for the lateral boundary effect is constructed. The resistance of the target with finite diameter RT is obtained by multiplying the decay function with the resistance of infinite target RT*. Then, a theoretical model for the penetration into the metal targets with finite diameters by long rods is proposed by substituting RT into the Alekseevskii–Tate model. The proposed model is verified by comparison with existing experimental results. Furthermore, the effect of the target diameter, initial impact velocity and yield criterion on the penetration depth and resistance are investigated. The results showed that the lateral boundary effect should be taken into account if the ratio of the target radius to the rod radius (rt0/RP) is less than 25.

To investigate the flavor characteristics of peanuts grown in Jiangsu, China, ten local varieties were selected. The amino acids, 5′-nucleotides and volatile substances were detected, and the flavor and odor characteristics of these varieties were estimated using an electronic tongue and nose. The results showed that the fat and protein contents of ten peanut varieties changed significantly (p < 0.05), and may have been negatively correlated with those of the Taihua 6 variety—in particular, having the highest protein content and the lowest fat content. The amino acid contents of the peanuts were 20.08 g/100 g (Taihua 4)–27.18 g/100 g (Taihua 6). Taihua 6 also contained the highest bitter (10.41 g/100 g) and sweet (6.06 g/100 g) amino acids, and Taihua 10 had the highest monosodium glutamate-like amino acids (7.61 g/100 g). The content of 5′-nucleotides ranged from 0.08 mg/g (Taihua 9725) to 0.14 mg/g (Taihua 0122–601). Additionally, 5′-cytidylate monophosphate (5′-CMP) and 5′-adenosine monophosphate (5′-AMP) were the major 5′-nucleotides detected in the peanuts. A total of 42 kinds of volatile flavor compounds were detected, with both Taihua 4 and 6 showing the most (18 kinds) and the highest content being in Taihua 4 (7.46%). Both Taihua 9725 and 9922 exhibited the fewest kinds (nine kinds) of volatile components, and the lowest content was in Taihua 9725 (3.15%). Formic acid hexyl ester was the most abundant volatile substance in peanuts, and the highest level (3.63%) was detected in Taihua 7506. The electronic tongue and nose indicated that the greatest taste difference among the ten varieties of peanuts was mainly related to sourness, and Taihua 4 and Taihua 9922 had special taste characteristics. On the other hand, the greatest smell difference among the ten varieties of peanuts was mostly for methane and sulfur organic substances, and Taihua 0605-2 had a special and strong smell characteristic. In conclusion, the content and composition differences of the flavor substances of ten peanut varieties were responsible for their divergences in taste and smell. These results will provide guidelines for the further use (freshly consumed or processed) of these ten peanut varieties.

The discharge of sediment plumes, which occurs mainly in the two depth zones, has a critical impact on assessing the deep-sea environment. Therefore, it is necessary to establish the corresponding physical oceanography for the evolution of these sediment plumes. For a more accurate evolution estimation of the plumes, the model in this research is concerned with the dynamic interaction between the deep-sea mining vehicle (DSMV) and the sediment plumes on small scales (t ≤ 2 s), contributing to a focus on the vital physical mechanics of controlling the extent of these plumes. The sediment concentration and particle trajectories of the plume emissions were determined using the Lagrangian discrete phase model (DPM). The results show that (1) the wake structure of the DSMV wraps the plume vortex discharged from the rear of the vehicle and inhibits the lateral diffusion of the plume, (2) the length of the entire wake (Lw) increases exponentially as the relative discharge velocity of the plume (U*) increases, where U* is defined as the dimensionless difference between the traveling velocity of the DSMV and the discharge velocity of the plume, and (3) at the same traveling speed of the DSMV and U* less than 0.75, the dispersion of the sediment particles in the early discharge stage of the plume does not vary with the plume discharge rate. This will be beneficial for the more accurate monitoring of ecological changes in deep-sea mining activities and provide theoretical guidance for the green design of DSMVs.

Hydrogen sulfide (H2S) plays prominent multifunctional roles in the mediation of various physiological processes and stress responses to plants. In this study, hydroponic experiments were carried out to explore the effects of NaHS pretreatment on the growth of wheat (Triticum aestivum L.) under 50 μM cadmium (Cd). Compared with Cd treatment alone, 50 μM NaHS pretreatment increased the plant height, soluble sugar content of shoots and roots, and dry weight of shoots and roots under Cd stress, while the Cd concentration of shoots and roots was significantly reduced by 18.1% and 25.9%, respectively. Meanwhile, NaHS pretreatment protected the photosynthetic apparatus by increasing the net photosynthetic rate and PSII electron transportation rate of wheat leaves under Cd stress. NaHS pretreatment significantly increased the soluble sugar content to maintain the osmotic pressure balance of the leaf cells. The gene expression results associated with photosynthetic carbon assimilation and sucrose synthesis in wheat leaves suggested that the NaHS pretreatment significantly up-regulated the expression of TaRBCL, TaRBCS, and TaPRK, while it down-regulated the expression of TaFBA, TaSuSy, TaSAInv, and TaA/NInv. In summary, NaHS pretreatment improved the resistance of wheat seedlings under Cd stress by increasing the rate of photosynthesis and regulating the expression of genes related to sugar metabolism.

Background: There have not been any head-to-head prospective studies to compare the effects of different chemotherapy regimens as first-line treatments for unresectable pancreatic cancer (UPC). We aimed to compare the effectiveness of nab-paclitaxel plus gemcitabine, mFOLFIRINOX and gemcitabine plus oxaliplatin (GEMOX) as first-line treatments by using real-world data from Chinese patients. Methods: We retrospectively included patients with UPC treated with nab-paclitaxel plus gemcitabine, mFOLFIRINOX or GEMOX as a first-line treatment at Sun Yat-sen University Cancer Center. Overall survival (OS), progression-free survival (PFS), objective response rate (ORR) and disease control rate (DCR) were assessed. Results: A total of 117 patients were administered nab-paclitaxel plus gemcitabine (n = 62), mFOLFIRINOX (n = 30) or GEMOX (n = 25) as first-line chemotherapy. The median OS was 11.1, 10.1 and 10.2 months (p = 0.75) in the nab-paclitaxel plus gemcitabine, mFOLFIRINOX and GEMOX, respectively. The ORR was similar among the three groups (24%, 23% and 32%, p = 0.76) and the DCR was higher in the nab-paclitaxel-gemcitabine group (82%) than the other two groups (60% and 64%, p = 0.04). The most common adverse events of grade 3 or 4 were neutropenia (32%, 28% and 5%), peripheral neuropathy (13%, 16% and 0) and fatigue (9%, 16% and 5%). Febrile neutropenia occurred in 2%, 4% and 5% of the patients in the three groups. Conclusion: In the first line treatment of UPC, our results suggest that nab-paclitaxel plus gemcitabine was associated with a higher DCR than mFOLFIRINOX or GEMOX, while all groups demonstrated similar OS, PFS and ORR.

The research on seafloor massive sulfide (SMS) started relatively late, and the results on its breaking process are few. However, the breaking process contains evaluation indexes of safe, efficient and low-disturbance mining, so it is necessary to study the breaking process of seafloor massive sulfide. At the same time, the finite element method is used in most existing researches, and the system will automatically delete the failure element from the system during the simulation of rock-breaking, resulting in the inability to accurately obtain the chip state in the breaking process. In addition, SPH meshless method has unique advantages in dealing with large deformations of rock-breaking, but it has the problems of difficultly in boundary processing and serious computational time. In view of this, a hybrid discretization method of finite element method and smooth particle hydrodynamics (SPH) is proposed in this paper. On this basis, numerical simulation of a single-pick cutting seafloor massive sulfide based on the FEM-SPH adaptive coupling algorithm is carried out. Through the research in this paper, the regularity of the fragmentation process of polymetallic sulfides is obtained: firstly, the breaking process of seafloor massive sulfide experiences four stages: cutting-in of the pick, evolution of the high-stress zone, formation of the dense core, and the chips’ splash. Secondly, the three-dimensional forces on the pick change in fluctuation in the cutting process. Thirdly, the stress wave propagation is unbalanced and biased in the cutting process. Fourthly, the chips’ splash mainly has three directions: jet flow towards the opposite direction of the cutter cutting, spluttering perpendicular to the cutting surface of the pick, and sliding along the cutting surface. Finally, the chip mass is positively correlated with the cutting time. In this paper, a simulation framework for rock-breaking is proposed, and its advantages have been effectively verified.

The effective detection of environmental pollutants is very important to the sustainable development of human health and the environment. A luminescent Cd(II) coordination complex, {[Cd(dbtdb)(1,2,4-H3btc)]·0.5H2O}n (1) (dbtdb = 1-(2,3,5,6-tetramethyl-4-((2-(thiazol-4-yl)-2H-benzo[d]imidazol-3(3aH)-yl)methyl)benzyl)-2,7a-dihydro-2-(thiazol-4-yl)-1H-benzo[d]imidazole, 1,2,4-H3btc = 1,2,4-benzenetricarboxylic acid), was obtained by hydrothermal reactions. Complex 1 has a chain structure decorated with uncoordinated Lewis basic O and S donors and provides good sensing of Fe3+, Cr2O72−, and p-nitrophenol with fluorescence quenching through an energy transfer process. The calculated binding constants were 3.3 × 103 mol−1 for Fe3+, 2.36 × 104 mol−1 for Cr2O72−, and 9.3 × 103 mol−1 for p-nitrophenol, respectively. These results show that 1 is a rare multiresponsive sensory material for efficient detection of Fe3+, Cr2O72−, and p-nitrophenol.

In terms of the failure of giving considerations to both aesthetic ornamental and low-carbon function for the current disaster prevention and mitigation engineering. This study proposes the debris-disaster prevention baffles applicable to natural scenic areas which designed based on mangroves properties, to solve this problem by adopting bionic design method. The research methodology is as follows: (1) To propose a Six Elements and Ten Steps Design Method for extracting the critical bionic elements of mangrove plants that contributes to the prevention of winds and waves. (2) To construct a decision objective model based on the Analytic Hierarchy Process method (AHP). Prioritize the critical bionic design elements and build a geometric structure model. (3) To compare the disaster mitigation performance through numerical simulations, and thus select an optimal one for further studies. (4) To design the final disaster prevention product based on the above theoretical guidance, low-carbon concept, efficient protection orientation, and environment-friendly principles. This study indicates that the use of bionic design satisfies aesthetic ornamental, and low-carbon demands. The appliance of AHP avoids subjective one-sidedness in design process when considering the priority of bionic elements. The numerical simulation experiments adopted in this study aim to compare the blocking effect of different baffle models and achieve the optimization the performance in disaster prevention of traditional baffle groups. In this study, the bionic product design methodology is adopted for baffle design to solve existing aesthetic and environmental problems. The particle accumulation mass after the new baffles can be effectively reduced by 2–3 times compared to the traditional baffles. Furthermore, the new baffle is more aesthetically pleasing than the traditional ones.

This paper constructs an open economy dynamic stochastic general equilibrium (DSGE) model with oil to investigate the transmission mechanism and impact effects of oil price fluctuations driven by different factors on China’s macroeconomy using quarterly data from 1996 to 2019. The results show that the international crude oil supply-driven oil price decline promotes positive output growth in the short run through the positive cost effect of the supply channel, and the production regulation cost will dampen the incentive to invest in the new energy sector in the long run. Domestic economic development demand-driven oil price increases act on the demand channel, driving output and oil prices to fluctuate in the same direction, generating a negative real balance effect on the economy through the interest rate channel. The oil-specific demand driven by foreign nominal interest rate shocks is transmitted through the exchange rate channel, triggering imported inflation, lower aggregate demand, and lower output. Different sources of oil price fluctuations have different transmission mechanisms and thus differential effects. For this reason, based on the root causes of oil price fluctuations, policy recommendations to deal with international oil price fluctuations in the new situation are proposed at the supply level, demand level, and international level.

Enhanced nitrogen (N) deposition has shown significant impacts on forest greenhouse gas emissions. Previous studies have suggested that Chinese forests may exhibit stronger N2O sources and dampened CH4 sinks under aggravated N saturation. To gain a common understanding of the N effects on forest N2O and CH4 fluxes, many have conducted global-scale meta-analyses. However, such effects have not been quantified particularly for China. Here, we present a meta-study of the N input effects on soil N2O emission and CH4 uptake in Chinese forests across climatic zones. The results suggest that enhanced N inputs significantly increase soil N2O emission (+115.8%) and decrease CH4 uptake (−13.4%). The mean effects were stronger for N2O emission and weaker for CH4 uptake in China compared with other global sites, despite being statistically insignificant. Subtropical forest soils have the highest emission factor (2.5%) and may respond rapidly to N inputs; in relatively N-limited temperate forests, N2O and CH4 fluxes are less sensitive to N inputs. Factors including forest type, N form and rate, as well as soil pH, may also govern the responses of N2O and CH4 fluxes. Our findings pinpoint the important role of Southern Chinese forests in the regional N2O and CH4 budgets.

Background: Glioma is one of the most common malignant tumors of the central nervous system. Immune infiltration of tumor microenvironment was associated with overall survival in low grade glioma (LGG). However, effects of Tyrosine phosphatase receptor type N (PTPRN) on the progress of LGG and its correlation with tumor infiltration are unclear. Methods: Here, datasets of LGG were from The Cancer Genome Atlas (TCGA) and normal samples were from GTEx dataset. Gepia website and Human Protein Atlas (HPA) Database were used to analyze the mRNA and protein expression of PTPRN. We evaluated the influence of PTPRN on survival of LGG patients. MethSurv was used to explore the expression and prognostic patterns of single CpG methylation of PTPRN gene in LGG. The correlations between the clinical information and PTPRN expression were analyzed using logistic regression and Multivariate Cox regression. We also explored the correlation between PTPRN expression and cancer immune infiltration by TIMER. Gene set enrichment analysis (GSEA) was formed using TCGA RNA-seq datasets. Results: PTPRN mRNA and protein expression decreased in LGG compared to normal brain tissue in TCGA and HPA database. Kaplan-Meier analysis showed that the high expression level of PTPRN correlated with a good overall survival (OS) of patients with LGG. The Multivariate Cox analysis demonstrated that PTPRN expression and other clinical-pathological factors (age, WHO grade, IDH status, and primary therapy outcome) significantly correlated with OS of LGG patients. The DNA methylation pattern of PTPRN with significant prognostic value were confirmed, including cg00672332, cg06971096, cg01382864, cg03970036, cg10140638, cg16166796, cg03545227, and cg25569248. Interestingly, PTPRN expression level significantly negatively correlated with infiltrating level of B cell, CD4+ T cells, Macrophages, Neutrophils, and DCs in LGG. Finally, GSEA showed that signaling pathways, mainly associated with tumor microenvironment and immune cells, were significantly enriched in PTPRN high expression. Conclusion: PTPRN is a potential biomarker and correlates with tumor immune infiltration in LGG.

Heat shock transcription factors (HSFs) are a family of transcription regulators. Although HSFs’ functions in controlling the transcription of the molecular chaperone heat shock proteins and resistance to stresses are well established, their effects on the pathogenicity of plant pathogenic fungi remain unknown. In this study, we analyze the role of CgHSF1 in the pathogenicity of Colletotrichum gloeosporioides and investigate the underlying mechanism. Failure to generate the Cghsf1 knock-out mutant suggested that the gene is essential for the viability of the fungus. Then, genetic depletion of the Cghsf1 was achieved by inserting the repressive promoter of nitrite reductase gene (PniiA) before its coding sequence. The mutant showed significantly decrease in the pathogenicity repression of appressorium formation, and severe defects in melanin biosynthesis. Moreover, four melanin synthetic genes were identified as direct targets of CgHSF1. Taken together, this work highlights the role of CgHSF1 in fungal pathogenicity via the transcriptional activation of melanin biosynthesis. Our study extends the understanding of fungal HSF1 proteins, especially their involvement in pathogenicity.

An Ms7.0 earthquake struck Jiuzhaigou (China) on 8 August 2017. The epicenter was in the eastern margin of the Tibetan Plateau, an area covered by a dense time-varying gravity observation network. Data from seven repeated high-precision hybrid gravity surveys (2014–2017) allowed the microGal-level time-varying gravity signal to be obtained at a resolution better than 75 km using the modified Bayesian gravity adjustment method. The “equivalent source” model inversion method in spherical coordinates was adopted to obtain the near-crust apparent density variations before the earthquake. A major gravity change occurred from the southwest to the northeast of the eastern Tibetan Plateau approximately 2 years before the earthquake, and a substantial gravity gradient zone was consistent with the tectonic trend that gradually appeared within the focal area of the Jiuzhaigou earthquake during 2015–2016. Factors that might cause such regional gravitational changes (e.g., vertical crustal deformation and variations in near-surface water distributions) were studied. The results suggest that gravity effects contributed by these known factors were insufficient to produce gravity changes as big as those observed, which might be related to the process of fluid material redistribution in the crust. Regional change of the gravity field has precursory significance for high-risk earthquake areas and it could be used as a candidate precursor for annual medium-term earthquake prediction.

This study aimed to provide comparative information of pharmaceutical properties, including particle morphology and distribution uniformity, solubility, presence of residual solvent and insoluble particles, and antimicrobial activities, between brand-name meropenem (Mepem®, BNM) and its six generic products (GPs A-F) marketed in China. Particles of GP-A and -C in dry powder had similar diameters of BNM, while other GPs were larger. Only BNM and GP-A were completely dissolved within 100 s in the lab condition. No insoluble particles >25 μm in diameter were detected in BNM and GP-E. Regarding stability of GPs solutions evaluated by concentration of open-ring metabolites at 6 h and 8 h, BNM showed the lowest open-ringed metabolite concentrates. Residual solvent of acetone detected in one GP showed the maximum value, while ethanol and ethyl acetate were detected both in product E and product F. The concordance rates (%) of minimum inhibitory concentration (MIC) of each generic compared to BNM were 89.5, 85, 87.5, 88, 88.5, and 86.5, respectively, although no significant difference was reached in MIC. Pharmaceutical characteristic differences between the BNM and GPs identified in this study could provide insights into understanding the deviations in the drug manufacturing processes of generic drugs.

Casticin has wide-ranging functional activities, but its water solubility is poor in food products. Here, a nanoemulsion stabilized by Maillard whey protein isolate conjugates (MWPI) was fabricated to encapsulate casticin. The nanoemulsion, with an average diameter of 200 nm, possessed the capability to load 700 μg/g casticin. MWPI-stabilized nanoemulsion showed better stability than that of the WPI nanoemulsion during 4 weeks of storage. Both the inhibition effects of the casticin-loaded nanoemulsion on cancer cells and the process of cellular uptake were studied. Results revealed that the casticin-loaded nanoemulsion had better inhibitory activity in HepG2 and MCF-7 cells than free casticin. Cellular uptake of the nanoemulsion displayed a time-dependent manner. After the nanoemulsion passed into HepG2 and MCF-7 cells, it would locate in the lysosome but not in the nucleus. The main pathway for the nanoemulsion to enter HepG2 cells was pinocytosis, whereas, it entered MCF-7 predominantly through the clathrin-mediated pit. This work implies that MWPI-stabilized nanoemulsions could be utilized as an effective delivery system to load casticin and have the potential to be applied in the food and pharmaceutical industries.

Early and goal-directed management of complications and comorbidities is imperative to facilitate neurorecovery and to optimize outcomes of disorders of consciousness (DoC). This is the first large retrospective cohort study on the primary medical and neurological complications and comorbidities in persons with DoC. A total of 146 patients admitted to a specialized inpatient DoC rehabilitation program from 1 January 2014 to 31 October 2018 were included. The incidences of those conditions since their initial brain injuries were reviewed per documentation. They were categorized into reversible causes of DoC, confounders and mimics, and other medical/neurological conditions. The common complications and comorbidities included pneumonia (73.3%), pain (75.3%), pressure ulcers (70.5%), oral and limb apraxia (67.1%), urinary tract infection (69.2%), and 4-limb spasticity (52.7%). Reversible causes of DoC occurred very commonly. Conditions that may confound the diagnosis of DoC occurred at surprisingly high rates. Conditions that may be a source of pain occurred not infrequently. Among those that may diminish or confound the level of consciousness, 4.8 ± 2.0 conditions were identified per patient. In conclusion, high rates of various complications and comorbidities occurred in persons with DoC. Correcting reversible causes, identifying confounders and mimics, and managing general consequences need to be seriously considered in clinical practice.

This is a retrospective study to investigate the results of using zolpidem and lorazepam in persons with disorders of consciousness (DoC) and to provide practical information for clinical application and further studies. The cohort included 146 patients (11 hemorrhagic stroke, 87 traumatic brain injury (TBI), 48 anoxic brain injury (ABI)) admitted to a specialized DoC rehabilitation program. A positive trial indicated a patient responded to either zolpidem or lorazepam with prominent functional improvements necessitating routine use of the medication. Non-responders had equivocal or negative (i.e., went to sleep) responses. Eleven patients with a stroke who had either medication were all non-responders. Of the remaining 135 patients, 95 received at least one medication trial. The overall positive rate was 11.6% (11/95), with 6.3% (5/79) for zolpidem and 14.0% (6/43) for lorazepam. Among TBI patients, the positive rate of the zolpidem trial (10.2%, 5/49) was slightly higher than that of the lorazepam trial (6.9%, 2/29; p > 0.05). Among ABI patients, the positive rate of the lorazepam trial (28.6%, 4/14) was significantly higher than that of the zolpidem trial (0%, 0/30; p = 0.007). Following a positive trial, most patients were continued on the medications on a regular basis before eventual discontinuation. Our results suggested the etiology of DoC, considering traumatic vs. anoxic injuries, may serve in guiding the clinical application of these medications in the treatment of DoC and in future prospective studies. We advocate for screening all patients with DoC using zolpidem and/or lorazepam.

Green petroleum coke, a form of industrial waste produced in the oil-refining process, was used to synthesize nitrogen-doped graphene-like plates (N-GLPs) together with melamine. In this study, characterization and batch experiments were performed to elucidate the interaction mechanism of N-GLPs and bisphenol A (BPA). Structural analysis of N-GLPs, including scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), Brunauer-Emmett-Teller (BET), and X-ray photoelectron spectroscopy (XPS), showed an obvious graphene-like structure and successful nitrogen doping. In addition, compared with 8.0 m2/g for green petroleum coke, the BET surface area of N-GLPs markedly increased to 96.6 m2/g. The influences of various factors, including contact time, temperature, and initial pH on BPA removal efficiency were investigated. It was found that 92.0% of BPA was successfully removed by N-GLPs at 50 °C. Based on the adsorption experiments, it was shown that electrostatic attraction, hydrogen bonding, and π-π interaction enhanced the adsorption capacity of N-GLPs for BPA. According to the thermodynamic data, the adsorption process was spontaneous, physical, and endothermic in nature. Therefore, N-GLPs are efficient adsorbent material to remove BPA from wastewater.

Nicosulfuron (NS) is a widely used sulfonylurea herbicide because of its high selectivity, broad spectrum of herbicide activity, and excellent performance. In this work, nicosulfuron methanol solvate (NS-MeOH) and [[3-[(Dimethylamino)carbonyl]-2-pyridinyl]sulfonyl]carbamic acid methyl ester (PCM) as a product of methanolysis of NS were obtained. Both of their structures were determined by a single crystal X-ray diffraction. A broad range of analytical techniques was applied to characterize the NS-MeOH, such as Powder X-ray diffraction (PXRD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), and hot stage microscopy (HSM). Combined with the analysis of the Independent gradient model (IGM), Atom-in-molecules (AIM), and Hirshfeld surface (HS), direct insights into the role of solvent played in the formation of NS-MeOH and the mechanism of solid-to-solid phase transformation of NS-MeOH could be obtained. In addition, the aqueous solubility of NS was improved through the formation of NS-MeOH. A systematic investigation of herbicidal activity of NS and PCM was carried out. It was found that NS and NS-MeOH had similar herbicidal activities at the experimental concentrations while PCM exhibited significantly lower activity. It was suggested that methanolysis of the sulfonylurea bridge in the NS molecule exerted a great influence on the herbicidal activity.

Emergency transportation plays a vital role in the success of disaster rescue and relief operations, but its planning and scheduling often involve complex objectives and search spaces. In this paper, we conduct a survey of recent advances in bio-inspired meta-heuristics, including genetic algorithms (GA), particle swarm optimization (PSO), ant colony optimization (ACO), etc., for solving emergency transportation problems. We then propose a new hybrid biogeography-based optimization (BBO) algorithm, which outperforms some state-of-the-art heuristics on a typical transportation planning problem.

Based on the graph theory and stability theory of dynamical system, this paper studies the stability of the trivial solution of a coupled fractional-order system. Some sufficient conditions are obtained to guarantee the global stability of the trivial solution. Finally, a comparison between fractional-order system and integer-order system ends the paper.

A novel bistable electromagnetic vibration energy harvester (BEMH) is constructed and optimized in this study, based on a nonlinear system consisting mainly of a flexible membrane and a magnetic spring. A large-amplitude transverse vibration equation of the system is established with the general nonlinear geometry and magnetic force. Firstly, the mathematical model, considering the higher-order nonlinearities given by nonlinear Galerkin method, is applied to a membrane with a co-axial magnet mass and magnetic spring. Secondly, the steady vibration response of the membrane subjected to a harmonic base motion is obtained, and then the output power considering electromagnetic effect is analytically derived. On this basis, a parametric study in a broad frequency domain has been achieved for the BEMH with different radius ratios and membrane thicknesses. It is demonstrated that model predictions are both in close agreement with results from the finite element simulation and experiment data. Finally, the proposed efficient solution method is used to obtain an optimizing strategy for the design of multi-stable energy harvesters with the similar flexible structure.

1,1,1,3,3,3-Hexafluoropropan-2-ol (HFIP) was found to be effective for the Bischler indole synthesis under microwave irradiation in the absence of a metal catalyst. Under the catalysis of HFIP, a wide range of α-amino arylacetones were successfully transformed into indole derivatives with moderate to good yields.

Diabetes mellitus is a chronic degenerative disease that causes long-term complications and represents a serious public health problem. In this manuscript, acankoreagenin isolated from the leaves of Acanthopanax gracilistylus (LAG) is thought to possess excellent anti-diabetic properties. In vitro, anti-diabetic activities were assessed based on the inhibitory activities with α-glucosidase (IC50 13.01 μM), α-amylase (IC50 30.81 μM), and PTP1B (IC50 16.39 μM). Acankoreagenin showed better anti-diabetic effects. Then, an investigation was performed to analyze the insulin secretion effects of the insulin-secreting cell line in RIN-m5F cells. It was found that acankoreagenin could increase the insulin release in RIN-m5F cells. It was also found that acankoreagenin reduced NO production, activity of caspase-3, and the reactive oxygen species levels in the cells injured by processing of cytokines. In western blotting, inactivation of NF-κB signaling was confirmed. Acankoreagenin (20 μM) showed a higher I-κBα expression and lower NF-κB expression than the control group and showed a better expression than the positive control L-NAME (1 mM) (p < 0.05). This study demonstrates the anti-diabetic effects of acankoreagenin in vitro and suggests acankoreagenin might offer therapeutic potential for treating diabetes mellitus.