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Selenium (Se) deficiency in human and animal nutrition is primarily due to low levels of Se in soils. It can be prevented by enriching crops, such as wheat, with Se through agronomic biofortification. Although Se is not essential for plants, it shows a dual effect on their metabolism depending on its concentration. This study aimed to elucidate the impact of five different concentrations (0.4, 4, 20, 40, and 400 mg/kg) of selenate and selenite on the oxidative status and antioxidative response of wheat (Triticum aestivum L., cv. Kraljica) shoots and roots. According to morpho-physiological analyses, selenite was found to have a lower toxicity threshold than selenate. The measurement of oxidative stress biomarkers showed that Se did not cause oxidative damage to wheat seedlings due to the activation of detoxification mechanisms at the biochemical level, which depended on the type of tissue, concentration, and form of applied Se. Treatment with 20 mg/kg of selenate can be recommended for wheat seedling biofortification due to a sufficient increase in Se accumulation in shoots without signs of toxicity. These results contribute to a better understanding of wheat seedlings’ physiological and biochemical responses to Se and the development of more effective biofortification strategies.

Agricultural production is already, and obviously, affected by climate change. Adapting to climate change includes reducing future risks to ensure yield quality and quantity and considers seizing any potential opportunities induced by climate change. In higher latitude areas, such as Norway, cold climate limits the cultivation of fruits. An increase in temperature offers more favorable conditions for fruit production. In this study, using available phenological observations (full blooming) and harvest dates, and meteorological data from the experimental orchard of NIBIO Ullensvang, the minimum heat requirements for growing different apple varieties are determined. Those criteria are used for zoning of the areas with heat favorable conditions for apple growing. Data on six varieties were used, with lower and higher requirements for heat for fruit development (Discovery, Gravenstein, Summerred, Aroma, Rubinstep, and Elstar). High resolution daily temperature data were generated and used for zoning of the areas with heat favorable conditions for apple growing within the selected domain, which includes Western Norway, Southern Norway, Eastern Norway, and the western part of Trøndelag, Mid-Norway. Dynamics of the change in such surfaces was assessed for the period of 1961–2020. The total surface with favorable heat conditions for growing the varieties with lesser requirement for heat increased three times during this period. The growing of more heat-demanding varieties increased from near zero to about 2.5% of the studied land surface. In the period of 2011–2020, surface area with favorable heat conditions for apple growing was almost 27,000 km2, and a surface area of about 4600 km2 can sustain growing of more heat-demanding varieties. The presented results show the increasing potential of the climate of Norway for apple cultivation and highlight the importance of implementation of fruit production planned according to climate change trends, including the assessment of potential risks from climate hazards. However, the methodology for determining heat requirements can be improved by using phenological ripening dates if available, rather than harvest dates which are impacted by human decision. Zoning of areas with the potential of sustainable apple growing requires the use of future climate change assessments and information on land-related features.

The commercial apple production in Norway is limited to the small regions along the fjords in the southwest part of the country and around lakes or near the sea in the southeast with favorable climate. Due to the rapid rate of climate change over the recent decades, it is expected that suitable heat conditions for apple growing will expand to the areas that were previously too cold. This study analyses the heat suitability of future climate (2021–2100) under the RCP8.5 scenario for 6 common apple varieties in Norway: Discovery, Gravenstein, Summerred, Aroma, Rubinstep and Elstar. Previously established heat requirement criteria (based on the temperature threshold for the full blooming and growing degree days sum between the full bloom and harvest) are applied to the temperature outputs of the regional climate models downscaled to 1 km resolution. The assessment indicates that as temperature rises, heat conditions suitable for cultivation of all 6 apple varieties will expand. According to the ensemble median value, areas with the favorable heat conditions for growing at least one of the considered apple varieties will increase 25 times in the period 2021–2040 and 60 times in the period 2041–2060, compared to the referent period 1971–2000. At the same time, areas suitable for all 6 apple varieties will increase 3 times in the first, and 3.8 times in the latter period. The favorable areas will advance from south and southeast northwards and inland in the eastern region, along the west and northwestern coastline towards higher latitudes, and along continental parts of fjords. The fastest expansion of heat suitable conditions is expected for Discovery and Gravenstein. The findings of this study are relevant for zoning apple production future potential and for strategical planning of climate change adaptation measures within the sector. Weather-related risks, such as risks from winter low temperatures, spring frost, drought and extreme precipitation were not considered.

The Integrated Agrometeorological Prediction System (IAPS) was a two-year project for the development of the long term forecast (LRF) for agricultural producers. Using LRF in decision-making, to reduce the risks and seize the opportunities, represents short-term adaptation to climate change. High-resolution ensemble forecasts (51 forecasts) were made for a period of 7 months and were initiated on the first day of each month. For the initial testing of the capacity of LRF to provide useful information for producers, 2017 was chosen as the test year as it had a very hot summer and severe drought, which caused significant impacts on agricultural production. LRF was very useful in predicting the variables which bear the memory of the longer period, such are growing degree days for the prediction of dates of the phenophases’ occurrences and the soil moisture of deeper soil layers as an indicator for the drought. Other project activities included field observations, communication with producers, web portal development, etc. Our results showed that the selected priority forecasting products were also identified by the producers as being the highest weather-related risks, the operational forecast implementation with the products designed for the use in agricultural production is proven to be urgent and necessary for decision-making, and required investments are affordable. The total cost of the full upgrade of agrometeorological climate services to meet current needs (including monitoring, seamless forecasting system development and the development of tools for information dissemination) was found to be about three orders of magnitude lower than the assessed losses in agricultural production in the two extreme years over the past decade.

Fusarium head blight and inadequate nitrogen fertilization can cause numerous biochemical changes in wheat. The main aim of this study was to determine the effects of Fusarium inoculation and a broader range of different nitrogen fertilization on the defense response in the spikes of four wheat varieties, varying in FHB susceptibility. Total soluble phenolics content, activities of enzymes involved in phenol metabolism (PAL and PPO), and total antioxidant capacity were determined as indicators of defense response. In both growing seasons, Fusarium inoculation altered PHE content in wheat spikes, indicating involvement of PHE in the defense response to Fusarium attack. Increased PHE content in the partially resistant varieties (Apache and Graindor) indicates involvement of PHE in the defense response and better disease tolerance in the more resistant varieties. Breeding wheat varieties with enhanced PHE synthesis could be a promising strategy to control FHB. To the best of our knowledge, this is the first study that emphasizes the effects of Fusarium infection and a broader range of different nitrogen fertilization on PHE and enzymes involved in PHE metabolism. In addition, this is the first study using the FRAP method to determine the antioxidant capacity of wheat tissues under the influence of Fusarium infection and different nitrogen fertilization.

Climate change, through changes in temperature, precipitation, and frequency of extreme events, has influenced agricultural production and food security over the past several decades. In order to assess climate and weather-related risks to fruit and grape production in Serbia, changes in bioclimatic indices and frequency of the occurrence of unfavourable weather events are spatially analysed for the past two decades (1998–2017) and the standard climatological period 1961–1990. Between the two periods, the Winkler and Huglin indices changed into a warmer category in most of the viticultural regions of Serbia. The average change shift was about 200 m towards higher elevations. Regarding the frequency of spring frost, high summer temperatures and water deficit, the most vulnerable regions in terms of fruit and grape production are found alongside large rivers (Danube, Sava, Great and South Morava), as well as in the northern part of the country. Regions below 300 m are under increased risk of high summer temperatures, as the number and duration of occurrences increased significantly over the studied periods. The high-resolution spatial analysis presented here gives an assessment of the climate change influence on the fruit and grapes production. The presented approach may be used in regional impact assessments and national planning of adaptation measures, and it may help increase resilience of agricultural production to climate change.

Due to climate change in recent years, there has been an increasing water deficit during the winter wheat sowing period. This study evaluated six Croatian winter wheat varieties’ physiological, biochemical, and molecular responses under two drought stress levels at the germination/seedling growth stage. Lipid peroxidation was mainly induced under both drought stress treatments, while the antioxidative response was variety-specific. The most significant role in the antioxidative response had glutathione along with the ascorbate-glutathione pathway. Under drought stress, wheat seedlings responded in proline accumulation that was correlated with the P5CS gene expression. Expression of genes encoding dehydrins (DHN5, WZY2) was highly induced under the drought stress in all varieties, while genes encoding transcription factors were differentially regulated. Expression of DREB1 was upregulated under severe drought stress in most varieties, while the expression of WRKY2 was downregulated or revealed control levels. Different mechanisms were shown to contribute to the drought tolerance in different varieties, which was mainly associated with osmotic adjustment and dehydrins expression. Identifying different mechanisms in drought stress response would advance our understanding of the complex strategies contributing to wheat tolerance to drought in the early growth stage and could contribute to variety selection useful for developing new drought-tolerant varieties.

In this study, we evaluated the leaf antioxidative responses of three wheat varieties (Srpanjka, Divana, and Simonida) treated with two different forms of zinc (Zn), Zn-sulfate and Zn-EDTA, in concentrations commonly used in agronomic biofortification. Zn concentration was significantly higher in the flag leaves of all three wheat varieties treated with Zn-EDTA compared to control and leaves treated with Zn-sulfate. Both forms of Zn increased malondialdehyde level and total phenolics content in varieties Srpanjka and Divana. Total glutathione content was not affected after the Zn treatment. Zn-sulfate increased the activities of glutathione reductase (GR) and guaiacol peroxidase (GPOD) in both Srpanjka and Divana, while glutathione S-transferase (GST) was only induced in var. Srpanjka. Chelate form of Zn increased the activities of GST and GPOD in both Simonida and Divana. Catalase activity was shown to be less sensitive to Zn treatment and was only induced in var. Srpanjka treated with Zn-EDTA where GPOD activity was not induced. Concentrations of Zn used for agronomic biofortification can induce oxidative stress in wheat leaves. The antioxidative status of wheat leaves could be a good indicator of Zn tolerance, whereas wheat genotype and chemical form of Zn are the most critical factors influencing Zn toxicity.

Industrialization and urbanization have led to an increased accumulation of waste materials that are transformed into a nutrient-rich and high-quality product called vermicompost by the vermicomposting process. Vermicomposting is an ecofriendly and economically favorable biotechnological process that involves the interaction of earthworms and microorganisms. Due to the importance of this process and its great potential in dealing with the consequences of waste accumulation, this review aims to provide key insights as well as highlight knowledge gaps. It is emphasized that there is a great challenge in understanding and clarifying the mechanisms involved in the vermicomposting process. The optimization of the factors affecting the possible application of vermicompost is crucial for obtaining the final product. Information on the composition of bacterial communities, amount of vermicompost, effect on heavy metal content, plant pathogens, diseases and organic waste selection is here recognized as currently the most important issues to be addressed. By answering these knowledge gaps, it is possible to enable wider utilization of vermicompost products.

Abiotic and biotic stresses, such as mineral nutrition deficiency (especially nitrogen) and Fusarium attack, pose a global threat with devastating impact on wheat yield and quality losses worldwide. This preliminary study aimed to determine the effect of Fusarium inoculation and two different nitrogen levels on oxidative status and antioxidative response in nine wheat varieties. Level of lipid peroxidation, activities of antioxidant enzymes (catalase, ascorbate peroxidase, glutathione reductase), phenolics, and chloroplast pigments content were measured. In general, wheat variety, nitrogen, and Fusarium treatment had an impact on all tested parameters. The most significant effect had a low nitrogen level itself, which mostly decreased activities of all antioxidant enzymes and reduced the chloroplast pigment content. At low nitrogen level, Fusarium treatment increased activities of some antioxidative enzymes, while in a condition of high nitrogen levels, antioxidative enzyme activities were mostly decreased due to Fusarium treatment. The obtained results provided a better understanding on wheat defense mechanisms against F. culmorum, under different nitrogen treatments and can serve as an additional tool in assessing wheat tolerance to various environmental stress conditions.

Chia (Salvia hispanica L.) is a one-year plant known as a source of nutrients that can be consumed in the diet in the form of seeds or sprouts. The purpose of this study is to investigate the effect of illumination for 24 and 48 h on dark-grown chia microgreens. Total antioxidant capacity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric-reducing antioxidant power (FRAP) assays, along with the total phenolics, ascorbic acid and cellulose content, and chlorophyll and carotenoid concentrations. Fourier transform infrared spectroscopy (FTIR) was used to evaluate the biochemical composition and elucidate the changes in compound structures between dark-grown and illuminated chia microgreens. Analysis of the results showed that illumination significantly increased the content of all measured bioactive compounds as well as antioxidative capacity, especially 48 h after exposure to light. FTIR analyses supported structural and molecular changes in chia microgreens grown under different light regimes. Our results suggest that illumination has a positive effect on the antioxidant potential of chia microgreens, which may present a valuable addition to the human diet.

The mineralogical composition of airborne dust particles is an important but often neglected parameter for several physiochemical processes, such as atmospheric radiative transfer and ocean biochemistry. We present the development of the METAL-WRF module for the simulation of the composition of desert dust minerals in atmospheric aerosols. The new development is based on the GOCART-AFWA dust module of WRF-Chem. A new wet deposition scheme has been implemented in the dust module alongside the existing dry deposition scheme. The new model includes separate prognostic fields for nine (9) minerals: illite, kaolinite, smectite, calcite, quartz, feldspar, hematite, gypsum, and phosphorus, derived from the GMINER30 database and also iron derived from the FERRUM30 database. Two regional model sensitivity studies are presented for dust events that occurred in August and December 2017, which include a comparison of the model versus elemental dust composition measurements performed in the North Atlantic (at Izaña Observatory, Tenerife Island) and in the eastern Mediterranean (at Agia Marina Xyliatos station, Cyprus Island). The results indicate the important role of dust minerals, as dominant aerosols, for the greater region of North Africa, South Europe, the North Atlantic, and the Middle East, including the dry and wet depositions away from desert sources. Overall, METAL-WRF was found to be capable of reproducing the relative abundances of the different dust minerals in the atmosphere. In particular, the concentration of iron (Fe), which is an important element for ocean biochemistry and solar absorption, was modeled in good agreement with the corresponding measurements at Izaña Observatory (22% overestimation) and at Agia Marina Xyliatos site (4% overestimation). Further model developments, including the implementation of newer surface mineralogical datasets, e.g., from the NASA-EMIT satellite mission, can be implemented in the model to improve its accuracy.

Icelandic topsoil sediments, as confirmed by numerous scientific studies, represent the largest and the most important European source of mineral dust. Strong winds, connected with the intensive cyclonic circulation in the North Atlantic, induce intense emissions of mineral dust from local sources all year and carry away these fine aerosol particles for thousands of kilometers. Various impacts of airborne mineral dust particles on local air quality, human health, transportation, climate and marine ecosystems motivated us to design a fully dynamic coupled atmosphere–dust numerical modelling system in order to simulate, predict and quantify the Icelandic mineral dust process including: local measurements and source specification over Iceland. In this study, we used the Dust Regional Atmospheric Model (DREAM) with improved Icelandic high resolution dust source specification and implemented spatially variable particle size distribution, variable snow cover and soil wetness. Three case studies of intense short- and long-range transport were selected to evaluate the model performance. Results demonstrated the model’s capability to forecast major transport features, such as timing, and horizontal and vertical distribution of the processes. This modelling system can be used as an operational forecasting system, but also as a reliable tool for assessing climate and environmental Icelandic dust impacts.

On 2 June 2014, at about 13 UTC, a dust storm arrived in Tehran as a severe hazard that caused injures, deaths, failures in power supply, and traffic disruption. Such an extreme event is not considered as common for the Tehran area, which has raised the question of the dust storm’s origin and the need for increasing citizens’ preparedness during such events. The analysis of the observational data and numerical simulations using coupled dust-atmospheric models showed that intensive convective activity occurred over the south and southwest of Tehran, which produced cold downdrafts and, consequently, high-velocity surface winds. Different dust source masks were used as an input for model hindcasts of the event (forecasts of the past event) to show the capability of the numerical models to perform high-quality forecasts in such events and to expand the knowledge on the storm’s formation and progression. In addition to the proven capability of the models, if engaged in operational use to contribute to the establishment of an early warning system for dust storms, another conclusion appeared as a highlight of this research: abandoned agricultural areas south of Tehran were responsible for over 50% of the airborne dust concentration within the dust storm that surged through Tehran. Such a dust source in the numerical simulation produced a PM10 surface dust concentration of several thousand μm/m3, which classifies it as a dust source hot-spot. The produced evidence indivisibly links issues of land degradation, extreme weather, environmental protection, and health and safety.