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Rice bacterial blight (BB), caused by Xanthomonas oryzae pv. oryzae (Xoo), is a major threat to rice production and food security. Exploring new resistance genes and developing varieties with broad-spectrum and high resistance has been a key focus in rice disease resistance research. In a preliminary study, rice cultivar Fan3, exhibiting high resistance to PXO99A and susceptibility to AH28, was developed by enhancing the resistance of Yuehesimiao (YHSM) to BB. This study performed a transcriptome analysis on the leaves of Fan3 and YHSM following inoculation with Xoo strains AH28 and PXO99A. The analysis revealed significant differential expression of 14,084 genes. Among the transcription factor (TF) families identified, bHLH, WRKY, and ERF were prominent, with notable differences in the expression of OsWRKY62, OsWRKY76, and OsbHLH6 across samples. Over 100 genes were directly linked to disease resistance, including nearly 30 NBS–LRR family genes. Additionally, 11 SWEET family protein genes, over 750 protein kinase genes, 63 peroxidase genes, and eight phenylalanine aminolysase genes were detected. Gene ontology (GO) analysis showed significant enrichment in pathways related to defense response to bacteria and oxidative stress response. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that differentially expressed genes (DEGs) were enriched in phenylpropanoid biosynthesis and diterpenoid biosynthesis pathways. Gene expression results from qRT-PCR were consistent with those from RNA-Seq, underscoring the reliability of the findings. Candidate genes identified in this study that may be resistant to BB, such as NBS–LRR family genes LOC_Os11g11960 and LOC_Os11g12350, SWEET family genes LOC_Os01g50460 and LOC_Os01g12130, and protein kinase-expressing genes LOC_Os01g66860 and LOC_Os02g57700, will provide a theoretical basis for further experiments. These results suggest that the immune response of rice to the two strains may be more concentrated in the early stage, and there are more up-regulated genes in the immune response of the high-resistant to PXO99A and medium-resistant to AH28, respectively, compared with the highly susceptible rice. This study offers a foundation for further research on resistance genes and the molecular mechanisms in Fan3 and YHSM.

With the development of miniaturization and integration of electrical and electronic equipment, the heat accumulation problems caused by the long-term operation of devices have become more and more serious. High thermal-conductivity and high-performance plastic composites have attracted significant interest from both academia and industry. Numerous studies have been recently conducted to enhance the thermal conductivity (TC) of nanofiller-filled polymeric composites. However, the homogeneous dispersion and directional arrangement of nanofillers in the resin matrix are the key factors limiting their effectiveness in enhancing thermal conductivity. Based on the feasibility considerations of mass production and industrial application, this paper reports on a novel preparation method of Poly(decamethylene terephthalamide)/graphite nanoparticle (GNP) nanocomposites with high thermal conductivity. Without borrowing solvents or other reagents, this method can effectively strip the inexpensive scaled graphite into nanoscale for its uniform dispersion and orientation arrangement by relying only on mechanical external forces. The whole technology is simple, green, and easy to industrialize. The fillers were well-dispersed and aligned in the PA10T, which played a role in significantly enhancing the thermal conductivity of the PA10T. In addition, we found that the thermal conductivity of the composites reached 1.20 W/(m·K) at 10 wt% filler content, which was 330% higher than that of the pure matrix. The mechanical properties of the composites were also significantly improved. This work provides guidance for the easy fabrication of thermally conductive composites with aligned structures.

Bacterial leaf blight (BLB), among the most serious diseases in rice production, is caused by Xanthomonas oryzae pv. oryzae (Xoo). Xa23, the broadest resistance gene against BLB in rice, is widely used in rice breeding. In this study, the rice variety CBB23 carrying the Xa23 resistance gene was inoculated with AH28 and PXO99A to identify differentially expressed genes (DEGs) associated with the resistance. Transcriptome sequencing of the infected leaves showed 7997 DEGs between the two strains at different time points, most of which were up-regulated, including cloned rice anti-blight, peroxidase, pathology-related, protein kinase, glucosidase, and other coding genes, as well as genes related to lignin synthesis, salicylic acid, jasmonic acid, and secondary metabolites. Additionally, the DEGs included 40 cloned, five NBS-LRR, nine SWEET family, and seven phenylalanine aminolyase genes, and 431 transcription factors were differentially expressed, the majority of which belonged to the WRKY, NAC, AP2/ERF, bHLH, and MYB families. Metabolomics analysis showed that a large amount of alkaloid and terpenoid metabolite content decreased significantly after inoculation with AH28 compared with inoculation with PXO99A, while the content of amino acids and their derivatives significantly increased. This study is helpful in further discovering the pathogenic mechanism of AH28 and PXO99A in CBB23 rice and provides a theoretical basis for cloning and molecular mechanism research related to BLB resistance in rice.

Honey bees have significant ecological and economic value as important pollinators, but they are continuously exposed to various environmental stressors, including insecticides, which can impair their health and cause colony decline. (1) Background: Cognitive abilities are vital for the functional maintenance of honey bees; however, it remains unknown if chronic, low-dose exposure to thiacloprid during the larval stage impairs the cognitive abilities of emerged adult honey bees. (2) Methods: To explore this question, honey bee larvae were fed 0, 0.5, and 1.0 mg/L thiacloprid during their developmental phase. Then, the cognitive (i.e., olfactory learning and memory) abilities of adult honey bees were quantified to assess the delayed impacts of early-stage thiacloprid exposure on adult honey bee cognition. Neural apoptosis and transcriptomic level were also evaluated to explore the neurological mechanisms underlying these effects. (3) Results: Our results revealed that chronic larval exposure to sublethal thiacloprid impaired the learning and memory abilities of adult honey bees by inducing neuronal apoptosis and transcriptomic alterations. (4) Conclusions: We highlighted a previously unknown impairment caused by thiacloprid in honey bees.

Poly(vinyl chloride) (PVC) is widely used in various fields and requires the use of thermal stabilizers to enhance its thermal stability during processing because of its poor thermal stability. Layered double hydroxides (LDHs) are widely considered to be one kind of highly efficient and environmentally friendly PVC thermal stabilizer. To investigate the thermal stabilizing process of layered double hydroxides (LDHs) in PVC resin, PVC and MgAl-LDHs powders with different interlayer anions (CO32−, Cl−, and NO3−) were physically mixed and aged at 180 °C. The structure of LDHs at different aging times was studied using XRD, SEM, and FT-IR. The results show that the thermal stabilizing process of LDHs on PVC mainly has three stages. In the first stage, the layers of LDHs undergo a reaction with HCl, which is released during the thermal decomposition of PVC. Subsequently, the ion exchange process occurs between Cl− and interlayer CO32−, resulting in the formation of MgAl-Cl-LDHs. Finally, the layers of MgAl-Cl-LDHs react with HCl slowly. During the thermal stabilizing process of MgAl-Cl-LDHs, the peak intensity of XRD reduces slightly, and no new XRD peak emerges. It indicates that only the first step happens for MgAl-Cl-LDHs. The TG-DTA analysis of LDHs indicates that the interaction of LDHs with different interlayer anions has the following order: NO3− < CO32− < Cl−, according to the early coloring in the thermal aging test of PVC composites. The results of the thermal aging tests suggest that LDHs with a weak interaction between interlayer anions and layers can enhance the early stability of PVC significantly. Furthermore, the thermal aging test demonstrates that LDHs with high HCl absorption capacities exhibit superior long-term stabilizing effects on PVC resin. This finding provides a valuable hint for designing an LDHs/PVC resin with a novel structure and excellent thermal stability.

The dynamic compression deformation of 3 µm, 15 µm and 25 µm grain size ZK60 alloys under high-strain-rate compression is systematically studied. Dynamic compression experiments at a strain rate of 1700 s−1 were conducted using a split Hopkinson pressure bar, and the microstructure of the specimen was characterized via electron backscatter diffraction and transmission electron microscopy, as well as via the calculation of Schmid factors. The results showed that the alloy exhibited the decrease in yield strength and peak stress as the grain size increased under dynamic compression. The grain refinement in the alloy was conducive to the activation of basic slip. In turn, an increase in the grain size caused the transition in the main deformation mechanism from pyramidal slip to {10−12} tensile twinning and pyramidal slip. Based on these deformation mechanisms, the Johnson-Cook constitutive equation with different grain sizes was modified, and the fitting results were in accordance with the experimental data.

2A55 Al-Li alloy is considered to have potential applications in the manufacture of fuel tanks for aerospace vehicles. However, the composition design of filler wires for 2A55 Al-Li alloy TIG welding has not been fully studied. This work focuses on the mechanical properties and microstructure of 2A55 Al-Li alloy TIG welded joints obtained using 2A55 alloy and Al-8Cu filler wire that was designed for this work, as well as the effects brought about by natural aging on them. When filler wire with higher Zr and Ti content was used, the weld grain was significantly refined. Due to the difference in the Cu content of filler wire, the formed Cu-rich phases in welds are significantly different, showing a grid-like distribution in the Al-8Cu weld compared to the dispersed distribution in the 2A55 weld. After welding, the lack of dominant strengthening precipitates caused a sharp softening of the welds. However, Li and Cu atoms dissolved in the Al matrix can precipitate fine and dispersed Al3Li particles and GPZs by natural aging to strengthen the welds. After natural aging, the yield strength (YS) of the 2A55 joint increased by 24.1% compared with the 14.7% improvement of the Al-8Cu joint.

In this paper, an effective method for preparing poly (p-phenylene terephthalamide) -co- poly (dodecanedioyl) decylamine (PA10T/1012)/graphene oxide (GO) composites by pre-dispersion and one-step in situ polymerization was proposed for the first time. During the process of polycondensation, the condensation between the terminal amino groups of PA10T/1012 chains and the oxygen-containing functional groups of GO allowed nylon to be grafted onto graphene sheets. The effects of polymer grafting on the thermal and mechanical properties of (PA10T/1012)/GO composites were studied in detail. Due to the interaction between PA10T/1012 grafted graphene sheets and its matrix, GO is well dispersed in the PA10T/1012 matrix and physically entangled with it, forming a cross-linked network structure of polymer bridged graphene, thus obtaining enhanced tensile strength, tensile modulus and impact strength. More importantly, benefiting from the cross-linked network structure, the heat distortion temperature (HDT) of the composite is greatly increased from 77.3 °C to 144.2 °C. This in situ polycondensation method opens a new avenue to prepare polycondensate graphene-based composites with high strength and high heat distortion temperatures.

Research on the cooperative adaptive cruise control (CACC) algorithm is primarily concerned with the longitudinal control of straight scenes. In contrast, the lateral control involved in certain traffic scenes such as lane changing or turning has rarely been studied. In this paper, we propose an adaptive cooperative cruise control (CACC) algorithm that is based on the Frenet frame. The algorithm decouples vehicle motion from complex motion in two dimensions to simple motion in one dimension, which can simplify the controller design and improve solution efficiency. First, the vehicle dynamics model is established based on the Frenet frame. Through a projection transformation of the vehicles in the platoon, the movement state of the vehicles is decomposed into the longitudinal direction along the reference trajectory and the lateral direction away from the reference trajectory. The second is the design of the longitudinal control law and the lateral control law. In the longitudinal control, vehicles are guaranteed to track the front vehicle and leader by satisfying the exponential convergence condition, and the tracking weight is balanced by a sigmoid function. Laterally, the nonlinear group dynamics equation is converted to a standard chain equation, and the Lyapunov method is used in the design of the control algorithm to ensure that the vehicles in the platoon follow the reference trajectory. The proposed control algorithm is finally verified through simulation, and validation results prove the effectiveness of the proposed algorithm.

Accurate passive 3D face reconstruction is of great importance with various potential applications. Three-dimensional polarization face reconstruction is a promising approach, but one bothered by serious deformations caused by an ambiguous surface normal. In this study, we propose a learning-based method for passive 3D polarization face reconstruction. It first calculates the surface normal of each microfacet at a pixel level based on the polarization of diffusely reflected light on the face, where no auxiliary equipment, including artificial illumination, is required. Then, the CNN-based 3DMM (convolutional neural network; 3D morphable model) generates a rough depth map of the face with the directly captured polarization image. The map works as an extra constraint to correct the ambiguous surface normal obtained from polarization. An accurate surface normal finally allows for an accurate 3D face reconstruction. Experiments in both indoor and outdoor conditions demonstrate that accurate 3D faces can be well-reconstructed. Moreover, with no auxiliary equipment required, the method ensures a total passive 3D face reconstruction.

This paper proposes a novel Squeeze-and-excitation-based Mask Region Convolutional Neural Network (SEM-RCNN) for Environmental Microorganisms (EM) detection tasks. Mask RCNN, one of the most applied object detection models, uses ResNet for feature extraction. However, ResNet cannot combine the features of different image channels. To further optimize the feature extraction ability of the network, SEM-RCNN is proposed to combine the different features extracted by SENet and ResNet. The addition of SENet can allocate weight information when extracting features and increase the proportion of useful information. SEM-RCNN achieves a mean average precision (mAP) of 0.511 on EMDS-6. We further apply SEM-RCNN for blood-cell detection tasks on an open source database (more than 17,000 microscopic images of blood cells) to verify the robustness and transferability of the proposed model. By comparing with other detectors based on deep learning, we demonstrate the superiority of SEM-RCNN in EM detection tasks. All experimental results show that the proposed SEM-RCNN exhibits excellent performances in EM detection.

Artificial water channels (AWCs) have been well investigated, and the imidazole-quartet water channel is one of the representative channels. In this work, covalent organic frameworks (COFs) composite membranes were fabricated through assembling COF layers and imidazole-quartet water channel. The membranes were synthesized by interfacial polymerization and self-assembly process, using polyacrylonitrile (PAN) ultrafiltration substrates with artificial water channels (HC6H) as modifiers. Effective combination of COF layers and imidazole-quartet water channels provide the membrane with excellent performance. The as-prepared membrane exhibits a water permeance above 271.7 L·m−2·h−1·bar−1, and high rejection rate (>99.5%) for CR. The results indicated that the composite structure based on AWCs and COFs may provide a new idea for the development of high-performance membranes for dye separation.

Most cities in China, especially industrial cities, are facing severe air pollution, which affects the health of the residents and the development of cities. One of the most effective ways to alleviate air pollution is to improve the urban ventilation environment; however, few studies have focused on the relationship between them. The Frontal Area Index (FAI) can reflect the obstructive effect of buildings on wind. It is influenced by urban architectural form and is an attribute of the city itself that can be used to accurately measure the ventilation capacity or ventilation potential of the city. Here, the FAIs of 45 industrial cities of different sizes in different climatic zones in China were computed, and the relationship between the FAI and the concentration of typical pollutants, i.e., NO2, were analyzed. It was found that (1) the FAIs of most of the industrial cities in China were less than 0.45, indicating that most of the industrial cities in China have excellent and good ventilation capacities; (2) there were significant differences in the ventilation capacities of different cities, and the ventilation capacity decreased from the temperate to the tropical climate zone and increased from large to small cities; (3) there was a significant difference in the ventilation capacity in winter and summer, indicating that that with the exception of building height and building density, wind direction was also the main influencing factor of FAI; (4) the concentration of NO2 was significantly correlated with the FAI, and the relative contribution of the FAI to the NO2 concentration was stable at approximately 9% and was generally higher than other socioeconomic factors. There was a turning point in the influence of the FAI on the NO2 concentration (0.18 < FAI < 0.49), below which the FAI had a strong influence on the NO2 concentration, and above which the influence of the FAI became weaker. The results of this study can provide guidance for suppressing urban air pollution through urban planning.

Soybean glycinin, as a major soybean allergen, is difficult to accurately quantify due to its large molecular weight and complex structure. CdSe/ZnS quantum dot nanobead (QB) is a core/shell fluorescent nanomaterial with strong fluorescent signals and high sensitivity at 630 nm. An immunosorbent assay based on CdSe/ZnS quantum dot nanobeads (QBs-FLISA) was developed for the glycinin quantification in soybean and soybean products. Here, the purified glycinin was coated on the microporous plate to serve as the coating antigen, and CdSe/ZnS nanobead conjugated with anti-glycinin polyclonal antibodies was used as fluorescent detection probe. The target glycinin in the sample and the coated antigen on the plate competitively adsorbed the antibody labeled the CdSe/ZnS QBs probes. The limits of detection and quantitation for glycinin were 0.035 and 0.078 μg mL−1, respectively. The recoveries of the spiked samples ranged from 89.8% to 105.6%, with relative standard deviation less than 8.6%. However, compared with ELISA, the sensitivities of QBs-FLISA for the detection of glycinin were increased by 7 times, and the detection time was shortened by two-thirds. This QBs-FLISA method has been effectively applied to the detection of soybean seeds with different varieties and soy products with different processing techniques, which will provide a rapid screening method for soybean and soybean products with low allergens.

Twelve Rho-related proteins (ROPs), namely PbROPs, were identified from the genome of the recently sequenced ‘Duli’ pear (Pyrus betulifolia Bunge), a wild-type pear variety routinely used for rootstocks in grafting in China. The length and molecular weight of these proteins are between 175 and 215 amino acids and 19.46 and 23.45 kDa, respectively. The 12 PbROPs are distributed on 8 of the 17 chromosomes, where chromosome 15 has the highest number of 3 PbROPs. Analysis of the deduced protein sequences showed that they are relatively conserved and all have the G domain, insertion sequence, and HVR motif. The expression profiles were monitored by quantitative RT-PCR, which showed that these 12 PbROP genes were ubiquitously expressed, indicating their involvement in growth and development throughout the life cycle of ‘Duli’ pear. However, they were altered upon treatments with abscisic acid (ABA, mimicking abiotic stress), polyethylene glycol (PEG, mimicking drought), and sodium chloride (NaCl, mimicking salt) to tissue-cultured seedlings. Further, transgenic Arabidopsis expressing PbROP1, PbROP2, and PbROP9 exhibited enhanced sensitivity to ABA, demonstrating that these 3 PbROPs may play important roles in the abiotic stress of ‘Duli’ pear. The combined results showed that the ‘Duli’ genome encodes 12 typical ROPs and they appeared to play important roles in growth, development, and abiotic stress. These preliminary data may guide future research into the molecular mechanisms of these 12 PbROPs and their utility in molecular breeding for abiotic stress-resistant ‘Duli’ pear rootstocks.

The hot deformation and dynamic recrystallization (DRX) characteristics of an as-extruded Mg-2.5Zn-4Y alloy containing long-period stacking ordered (LPSO) phases were investigated using a Gleeble 3500 thermal simulator at temperatures (300–400 °C) and strain rates (0.001–1 s−1). The results revealed that low flow stress corresponded to a high temperature and a low strain rate. An increase in the temperature of deformation caused an increase in the amount of dynamic recrystallization. Additionally, as the strain rate decreased at a given deformation temperature, dislocations were less likely to cause pile-up and dynamic recrystallization was more appropriate, resulting in a lower stress value. Kink deformation was clearly minimized as the number of dynamic recrystallizations increased. The test alloy’s activation energy value was determined as 212.144 kJ/mol.

In this study, the cavitation erosion behavior of 316L stainless steel under different cavitation intensities in 3.5 wt.% NaCl solution was investigated with scanning electron microscopy and various electrochemical tests. Results indicated that cavitation intensity corresponding to CE amplitude of 5 μm was lower than that of the mechanical bearing capacity of passive films. When subjected to cavitation erosion (CE), Open circuit potential (OCP) shifted to the noble direction instantaneously, which was mainly attributed to enhanced oxygen transfer of the cathode due to stirring effects of CE. By contrast, high cavitation intensities corresponding to CE amplitudes of 25 μm and 55 μm exceeded the mechanical bearing capacity of passive films, causing significantly reduced OCP associated with metal dissolution of the anode from mechanical damage. Potentiostatic polarization and Mott–Schottky tests showed that 316L SS subjected to low cavitation intensities displayed good repassivation properties. However, repassivation performance was markedly weakened when high cavitation intensity was applied, resulting in weaker protection of the passive films with high carrier density.

The growing population and northward shifts in the center of grain production collectively contribute to the arising farmland use intensity of the farming–pastoral ecotone of Northern China (FPENC). Consequently, it poses a great threat to the vulnerable ecosystem of FPENC. Thus, farmland use intensity monitoring is a top priority to practice sustainable farming. In this study, we establish an indicator system designed to evaluate farmland use intensity in Ulanqab, located in the central part of FPENC. This system includes three single-year indicators (the degree of coupling between effective rainfall and crop water requirement (Dcrr), irrigation intensity (Iri) and crop duration (Cd)) and two multi-year indicators (the frequency of adopting the green-depressing cropping system (Gf) and rotation frequency (Rf)). We mapped five farmland use intensity indicators in Ulanqab from 2010 to 2019 using satellite imagery and other ancillary data. Then, the farmland use patterns were recognized by applying the self-organizing map algorithm. Our results suggest that the mapping results of crop types, center pivot irrigation (CPI), and irrigated areas are reasonably accurate. Iri, Cd, and Rf experienced an increase of 31 m3/hm2, 1 day, and 0.06 in Ulanqab from 2010 to 2019, respectively, while Dcrr and Gf witnessed a decrease of 0.002 and 0.004, respectively. That is, farmers are progressively inclined to higher farmland use intensity. Moreover, spatial heterogeneity analysis shows that Northern Ulanqab owned higher Dcrr, Iri, Cd, and Rf, and lower Gf than the southern part. We conclude the paper by discussing the implications of the results for areas with different farmland use intensity patterns.

Watermelon is one of the most desirable vegetable crops in the world. Recently, grafting is common in watermelons worldwide, but not all grafting methods are compatible with polyploids. In this study, diploid, triploid, and tetraploid from one watermelon variety, “Mi Mei”, were grafted on the “Xijiaqiangsheng” squash rootstock to study the effect of genome duplication on graft compatibility. Three grafting methods (splice, hole, and tongue) were used to determine graft compatibility. Significant differences in survival rates, hormones, antioxidants (AOX), sugars, and starch contents were observed between compatible/incompatible combinations. Compatible combinations with high survival rates showed high levels of hormones, AOX, carbohydrates, and low hydrogen peroxide H2O2 compared to incompatible plants. The hole grafting method was more efficient with diploid, while splice was efficient with a tetraploid, and both methods can be used for triploid. Compatibility is a combined effect of hormone, carbohydrate, and antioxidant activities. We predict that compatibility is a complex process and that further molecular studies must be performed to dig deep into this phenomenon.

ZnS is a promising photocatalyst in water purification, whereas its low photon efficiency and poor visible-light response restrict its application. Constructing composites may help solve these problems. In this work, Ag2O was introduced to ZnS for the first time based on their energy band characteristics to form a novel ZnS/Ag2O composite photocatalyst. In the model reaction of degrading methylene blue, the as-designed catalyst exhibited high catalytic activity among a series of ZnS-based composite photocatalysts under similar conditions. The catalytic rate constant was up to 0.138 min−1, which is 27.4- and 15.6-times higher than those of ZnS and Ag2O. This composite degraded 92.4% methylene blue in 50 min, while the ratios were 31.9% and 68.8% for ZnS and Ag2O. Catalytic mechanism study based on photoluminescence and radical-scavenging experiments revealed that the enhanced photocatalytic activity was attributed to the composite structure of ZnS/Ag2O. The structure not only facilitated the separation and transmission of photogenerated carriers but also extended the light response range of the catalyst. The as-designed ZnS/Ag2O composite is promising in degrading organic pollutants in water.

Zinc oxide (ZnO) possesses superior chemical and physical properties so that it can occupy an essential position in the application of nanostructures. In this paper, ZnO nano-rod arrays were synthesized by a simple one-step hydrothermal approach with the assistance of cetyl trimethyl ammonium bromide (CTAB). Exposure of the {0001} facets could be controlled by adjusting the amount of CTAB and the maximum exposure of the {0001} facets of ZnO nanorods is obtained at 1.2 g of CTAB. The photocurrent, EIS, and PL measurements support the facile charge transfer with minimum recombination of the photogenerated excitons of the ZnO nano-rod arrays obtained at 1.2 g of CTAB. Consequently, the obtained ZnO nano-rod arrays at the optimal CTAB of 1.2 g exhibit an excellent photocatalytic degradation rate of 99.7% for rhodamine B (RhB), while the degradation rate of RhB by the ZnO obtained without CTAB is only 35%.

Circular polarization (CP) memory is a well-known phenomenon whereby natural light becomes partially circularly polarized after scattering by water spray several times, and the circularly polarized state can be well preserved within a certain propagation distance. In this study, a CP imaging method combined with the multi-scale analysis in the frequency domain is proposed to enhance the vision in rainy conditions. The images were first decomposed into multi-scales. CP characteristics of light were employed in the low-frequency parts to improve the quality of images in rainy conditions, and the high-frequency parts compensated specific structure information. Experimental results demonstrate that the proposed method can remove the water spray effect thereby improving the vision of degraded rainy-day images.

Watermelon (Citrullus lanatus) is one of the most nutritional fruits that is widely distributed in the whole world. The nutritional compositions are mainly influenced by the genotype and environment. However, the metabolomics of different domestication status and different flesh colors watermelon types is not fully understood. In this study, we reported an extensive assessment of metabolomic divergence in the fruit flesh among Citrullus sp. and within Citrullus sp. We demonstrate that metabolic profiling was significantly different between the wild and cultivated watermelons, the apigenin 6-C-glucoside, luteolin 6-C-glucoside, chrysoeriol C-hexoside, naringenin C-hexoside, C-pentosyl-chrysoeriol O-hexoside, and sucrose are the main divergent metabolites. Correlation analysis results revealed that flavonoids were present in one tight metabolite cluster. The main divergent metabolites in different flesh-colored cultivated watermelon fruits are p-coumaric acid, 2,3-dihydroflavone, catechin, N-(3-indolylacetyl)-l-alanine, 3,4-dihydroxycinnamic acid, and pelargonidin o-hexoside. A total of 431 differentially accumulated metabolites were identified from pairwise comparative analyses. C. lanatus edible-seed watermelon (cultivars) and C. mucosospermus (wild) have similar fruit metabolic profiles and phenotypic traits, indicating that edible-seed watermelon may be a relative of wild species and a relatively primitive differentiation type of cultivated watermelon. Our data provide extensive knowledge for metabolomics-based watermelon improvement of Citrullus fruits meet their enhanced nutritive properties or upgraded germplasm utility values.

Mg−7Gd−5Y−0.5Zr alloy has excellent mechanical properties but poor hot tearing resistance. The latter makes it difficult to cast billets, which limits the size of subsequently processed parts. Therefore, the hot tearing susceptibility of Mg−7Gd−5Y−xZn−0.5Zr (x = 0, 3, 5, 7 wt%) alloys was studied. It was found that Zn can significantly reduce hot tearing susceptibility of Mg−7Gd−5Y−0.5Zr alloy, which almost linearly decreased with Zn content. When Zn content was 3 wt%, 5 wt% and 7 wt%, hot tearing susceptibility will be reduced by 27%, 83% and 100%, respectively. It was further revealed that the solid solubility of Gd and Y in α-Mg decreased with the increase of Zn content, and the nucleation temperature decreased accordingly, which resulted in the increase of nucleation rate and the refinement of final grains. On the macro level, it showed that the dendrite coherency temperature decreased, the solidification shrinkage stress of α-Mg slowed down, and the residual liquid channel became shorter and hot tearing susceptibility decreased. It was also found that with the increase of Zn content, the content of Zn, Gd and Y enriched on the grain boundary increased, the content of residual liquid phase between dendrites increased after α-Mg crystallization, and the solidified precipitated second phase also changed from Mg5RE phase to long-period stacking ordered phase + W-phase (a little), long-period stacking ordered phase + W-phase (much) and finally to W-phase only. The feeding effect of sufficient intergranular residual liquid on the shrinkage of α-Mg dendrite and the bridging effect of the precipitated phase at the grain boundary (especially long-period stacking ordered phase which is coherent with the parent phase) also led to the decrease of hot tearing susceptibility.

Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expression levels were significantly upregulated in 30 μM Cu-Pro, 120 μM Cu-Gly, and 120 μM Cu-Pro treatment groups; intracellular reactive oxygen species (ROS) generation and malondialdehyde (MDA) content increased significantly in 30 μM treatment groups and 120 μM CuSO4 treatment group. CTR1 and ATP7A gene expression were significantly downregulated in the 120 μM exposed groups. While upregulation of ATOX1 expression was observed in the presence of 120 μM Cu-Gly and Cu-Pro. ASCT2 gene expression was significantly upregulated after 120 μM Cu-Glycine and CuSO4 exposure, and PepT1 gene expression was significantly upregulated after Cu-Pro exposure. In addition, CTR1 protein expression level decreased after 120 μM CuSO4 and Cu-Gly exposure. PepT1 protein expression level was only upregulated after 120 μM Cu-Pro exposure. These findings indicated that extra copper supplementation can induce intestinal epithelial cell injury, and different forms of copper may have differing effects on cell metabolism.

Photovoltaic output is affected by solar irradiance, ambient temperature, instantaneous cloud cluster, etc., and the output sequence shows obvious intermittent and random features, which creates great difficulty for photovoltaic output prediction. Aiming at the problem of low predictability of photovoltaic power generation, a combined photovoltaic output prediction method based on variational mode decomposition (VMD), maximum relevance minimum redundancy (mRMR) and deep belief network (DBN) is proposed. The method uses VMD to decompose the photovoltaic output sequence into modal components of different characteristics, and determines the main characteristic factors of each modal component by mRMR, and the DBN model is used to fit the modal components and the corresponding characteristic factors, then the predicted results of each modal component is superimposed to obtain the predicted value of the photovoltaic output. By using the data of a certain photovoltaic power station in Yunnan for comparative experiments, it is found that the model proposed in this paper improves the prediction accuracy of photovoltaic output.

N-carbamylglutamate (NCG), a synthetic analogue of N-acetylglutamate, is an activator of blood ammonia conversion and endogenous arginine synthesis. Here, we established an accurate quantitative determination of NCG in feeds, animal tissues, and body fluids using the high performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS). The sample pretreatment procedures included extraction with 0.5% of formic acid in water/methanol (80/20, v/v), and purification using an anionic solid phase extraction cartridge. Satisfactory separation of NCG was achieved in 20 min with the application of an Atlantis T3 column, and a confirmative detection of NCG was ensured by multiple reaction monitoring of positive ions. NCG spiked in feeds, tissues, and body fluids were evaluated in regard to linearity, sensitivity, recovery, and repeatability. Recoveries for different sample matrices were in the range of 88.12% to 110.21% with relative standard deviations (RSDs) less than 8.8%. Limits of quantification were within the range of 0.012 to 0.073 mg kg−1 and 0.047 to 0.077 μg mL−1 for solid and liquid samples, respectively. This study will provide a solid foundation for the evaluation of availability and metabolic mechanism of NCG in animals.

(E)-β-Ocimene was the only volatile chemical found to be emitted by whole, live worker larvae of Apis mellifera L. when sampling in the vapor phase. In addition to (E)-β-ocimene, there is evidence for the existence of other volatiles, but the changes in their composition and contents remain unknown during larval development, as are their differences from larvae to larval food. We investigated volatile components of worker larvae and larval food using solid phase dynamic extraction (SPDE) coupled with gas chromatography-mass spectrometry (GC-MS). Nine compounds were identified with certainty and six tentatively, including terpenoids, aldehydes, hydrocarbons, an ester and a ketone. The contents of volatiles in the second-instar worker larvae differ greatly from those in larvae of other stages. This is mainly attributable to terpenoids, which resulted in the second-instar worker larvae having significantly higher amounts of overall volatiles. Larval food contained significantly higher amounts of aldehydes and hydrocarbons than the corresponding larvae from the fourth to fifth-instar. We discovered volatiles in worker larvae and their food that were never reported before; we also determined the content changes of these volatiles during larval development.

A new prenylated benzofuran derivative, named flemiphilippinone A, was isolated together with ten known flavonoids from the roots of Flemingia philippinensis. Flemiphilippinone A was identified as (2S,3aS)-5-(1-hydroxy-3-(4-methoxyphenyl)- propylidene)-2-(2-hydroxypropan-2-yl)-3a,7-bis(3-methylbut-2-en-1-yl)tetrahydrobenzo- furan-4,6(2H,5H)-dione, and its structure was established by a combination of HR EIMS, 1H-NMR, 13C-NMR, HMQC, HMBC and NOESY spectra data.

Gly m 5.0101, the alpha subunit of β-conglycinin, is one of the major allergens found in soybeans that has been identified as causing an allergic reaction. Here, we developed a quantification method of Gly m 5.0101 with multiple reaction monitoring using the synthetic peptide 194NPFLFGSNR202 as the external standard. Firstly, the ground soybean was defatted and extracted with a protein extraction buffer. Then the crude extract was on-filter digested by trypsin and analyzed by liquid chromatography-tandem mass spectrometry. The selected peptide exhibited a detection limit of 0.48 ng/mL and a linear relationship in a concentration range from 1.6 to 500 ng/mL (r2 > 0.99). The developed method was successfully applied to quantify the Gly m 5.0101 level in dozens of soybean varieties from different sources and soybean products derived from different processing techniques. The developed method could be used to further analyze β-conglycinin in soybean seeds combined with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis.

Branched-chain keto acids (BCKAs) are derivatives from the first step in the metabolism of branched-chain amino acids (BCAAs) and can provide important information on animal health and disease. Here, a simple, reliable and effective method was developed for the determination of three BCKAs (α-ketoisocaproate, α-keto-β-methylvalerate and α-ketoisovalerate) in serum and muscle samples using high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-Q-TOF/MS). The samples were extracted using methanol and separated on a 1.8 μm Eclipse Plus C18 column within 10 min. The mobile phase was 10 mmol L−1 ammonium acetate aqueous solution and acetonitrile. The results showed that recoveries for the three BCKAs ranged from 78.4% to 114.3% with relative standard deviation (RSD) less than 9.7%. The limit of quantitation (LOQ) were 0.06~0.23 μmol L−1 and 0.09~0.27 nmol g−1 for serum and muscle samples, respectively. The proposed method can be applied to the determination of three BCKAs in animal serum and muscle samples.