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The present study aimed to contribute to the diagnosis and advance the knowledge of the impacts of land use change and climate change on the tropical longleaf forest biome at the continental scale in South America (Biome 1 according to the WWF classification) for realizing scientific progress in the search for convincing strategies and actions by different actors for the preservation of forests in the continent. The status and climate of the area, which harbors the tropical longleaf forests of South America, were assessed. Moreover, volumetric soil moisture (VSM) was evaluated through maps and simulation using the autoregressive integrated moving average model (ARIMA). Furthermore, future climate scenarios were predicted based on El Niño–Southern Oscillation phenomena, meteorological systems, and scientific evidence, such as the shared socioeconomic pathways (SSPs) and sociopolitical dynamics evident in the region from the case analysis of the Brazilian states of Acre and Rio de Janeiro. An increase was noted in the temperature and range of precipitation variation in the biome. ARIMA analysis indicated changes of up to 0.24 m3 m−3 and an increased range of future VSM values. The December–January–February (DJF) quarter recorded the highest VSM median with the measurement scale of 0.05 to 0.44 m3 m−3, while the June–July–August (JJA) quarter recorded the lowest value. The regions of the biome with the lowest VSM values included southern Amazon (Ecuador, Peru, and the Brazilian states of Acre, Mato Grosso, Pará, and Maranhão), Brazilian Atlantic Forest, Southeast Region, and the Brazilian state of Bahia.