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The present work shows that the exposure of mesothelial cells to simulated microgravity changes their cytoskeleton and adhesion proteins, leading to a cell switch from normal towards tumoral cells. Immunohistochemical and molecular data were obtained from both MeT-5A exposed to simulated microgravity and BR95 mesothelioma cell lines. Simulated microgravity was found to affect the expression of actin, vinculin, and connexin-43, altering their quantitative and spatial distribution pattern inside the cell. The analysis of the tumoral markers p27, CD44, Fibulin-3, and NANOG and the expression of genes related to cancer transformation such as NANOG, CDH-1, and Zeb-1 showed that the simulated microgravity environment led to expression patterns in MeT-5A cells similar to those observed in BR95 cells. The alteration in both quantitative expression and structural organization of the cytoskeleton and adhesion/communication proteins can thus be considered a pivotal mechanism involved in the cellular shift towards tumoral progression.

The abandonment of inhabited places is a phenomenon widespread on a global scale that has spanned centuries. It has led to the birth of the so-called ‘ghost towns’. These lifeless sites dot the internal Mediterranean and European areas, testifying to the changeability of the human settlements. Through a vision that reverses the paradigm that epitomises the ‘ghost towns’, these places can be transformed from a problem into an opportunity for the development of the territories that host them. The main topic of this article is to present and update investigations performed on three abandoned settlements sited in Portugal, Italy, and Albania in view of their tourist exploitation, considering three different tailored strategies: underwater tourism, dark tourism, and heritage tourism. For each site, we analysed the site history, the abandonment causes, and the territorial-geological features, thus arguing for the possible valorisation and enjoyment approaches with special attention to digital technologies, which are highly underexploited in the sector. This study, which is part of an international research landscape still in the initial stages, falls within the BEGIN project (aBandonment vErsus reGeneratIoN), which aims to develop a multilevel methodological–operational protocol useful in regional, national, EU, and non-EU contexts for the dissemination of knowledge, conservation, regeneration, valorisation, management, and fruition of areas classified as a ‘ghost town’. The research findings can be useful for scholars, practitioners, and local entities entrusted to manage the abandoned towns. Furthermore, the methodological approach followed in this research can supply useful insights into aims to valorise and enjoy worldwide ‘ghost towns’.

Investigational drug services need to be organised in a structured approach, especially for sites with a large number of ongoing clinical trials. The aim of this study was to develop a tool to assess the complexity of pharmacy involvement in a sponsored oncology clinical trial. Categorisation into ordinal complexity categories was used to assess the complexity of the clinical trials for consistent pharmacy grant applications. The 15 items of the tool were divided into three sections, and individual item scores were agreed upon among four pharmacists with experience in the conduct of clinical trials at two different centres. A final version of the tool, named Pharm-CAT, was approved. The pharmacists were instructed to use Pharm-CAT to assign a score to each new sponsored trial. To determine the cut-offs for the complexity categories, the scores were sorted in ascending order and the cut-offs corresponding to the first and third tertiles of the score distribution were selected. To verify the reproducibility of the results, Pharm-CAT was applied by two pharmacists independently for each trial. Pharm-CAT proved to be user-friendly. Sixty clinical trials were evaluated and a total of 120 scores were recorded. Low-complexity scores ranged from 0 to 19, medium-complexity scores ranged from 20 to 25, and high-complexity scores were 26 or higher. The average score recorded was 22.88 points. Prospective multicentre validation of Pharm-CAT is needed to confirm its applicability.

This study aimed to evaluate the impact of using an AI model, specifically ChatGPT-3.5, in remote sensing (RS) applied to archaeological research. It assessed the model’s abilities in several aspects, in accordance with a multi-level analysis of its usefulness: providing answers to both general and specific questions related to archaeological research; identifying and referencing the sources of information it uses; recommending appropriate tools based on the user’s desired outcome; assisting users in performing basic functions and processes in RS for archaeology (RSA); assisting users in carrying out complex processes for advanced RSA; and integrating with the tools and libraries commonly used in RSA. ChatGPT-3.5 was selected due to its availability as a free resource. The research also aimed to analyse the user’s prior skills, competencies, and language proficiency required to effectively utilise the model for achieving their research goals. Additionally, the study involved generating JavaScript code for interacting with the free Google Earth Engine tool as part of its research objectives. Use of these free tools, it was possible to demonstrate the impact that ChatGPT-3.5 can have when embedded in an archaeological RS flowchart on different levels. In particular, it was shown to be useful both for the theoretical part and for the generation of simple and complex processes and elaborations.

Gravity is a primary physical force that has a profound influence on the stability of the cell cytoskeleton. In our research, we investigated the influence of microgravity on altering the cytoskeletal pathways of glioblastoma cells. The highly infiltrative behavior of glioblastoma is supported by cytoskeletal dynamics and surface proteins that allow glioblastoma cells to avoid stable connections with the tissue environment and other cells. Glioblastoma cell line C6 was exposed to a microgravity environment for 24, 48, and 72 h by 3D-RPM, a laboratory instrument recognized to reproduce the effect of microgravity in cell cultures. The immunofluorescence for GFAP, vinculin, and Connexin-43 was investigated as signals related to cytoskeleton dynamics. The polymerization of GFAP and the expression of focal contact structured by vinculin were found to be altered, especially after 48 and 72 h of microgravity. Connexin-43, involved in several intracellular pathways that critically promote cell motility and invasion of glioma cells, was found to be largely reduced following microgravity exposure. In conclusion, microgravity, by reducing the expression of Connexin-43, alters the architecture of specific cytoskeletal elements such as GFAP and increases the focal contact, which can induce a reduction in glioma cell mobility, thereby inhibiting their aggressive metastatic behavior.

Sustainable energy projects can help societies reduce climate change’s negative impacts. In the present paper, published studies regarding European community energy schemes (CESs) were analysed to investigate CESs’ barriers and drivers towards including community members and, more specifically, vulnerable groups. This review followed the PRISMA guidelines to ensure transparency. Exclusion and inclusion criteria were defined to select the proper articles and books that were aligned with the scope of this review. A total of 143 publications from 2019 to February 2024 were considered and selected. Content analysis was used to outline the various definitions of energy poverty, vulnerable groups, and CESs as well as the different strategies and approaches followed by European countries to tackle energy poverty and increase public engagement in CESs. This research suggests that environmental citizenship is a concept that can bring individuals and society together to promote changes in energy-related behaviours. The outcome reveals factors that enhance community energy acceptance and social cohesion such as providing energy education or education for sustainability to community members, financial support, increased prosocial culture, and the participation of vulnerable groups in CESs. This can be achieved through various interventions to help community members, including vulnerable groups, understand sustainable energy behaviour and thus reduce energy poverty.

Even though Family and Community Nurses (FCNs) were introduced into the Italian healthcare system in 2000, to date, there is a substantial knowledge gap regarding the implementation of these professional figures within the Primary Care (PC) system. This scoping review aims to provide a comprehensive picture of the role of FCNs in managing older adults and the elderly with chronic conditions within the Italian PC system. A search on Medline, Cumulative Index to Nursing and Allied Health Literature, Cochrane Library, Embase, and Scopus was conducted including studies published until 7 April 2023. Among 141 potential articles, only 4 met our inclusion criteria. Each of these studies attributed their findings to the presence of FCNs. They reported a significant decrease in the prevalence of several behaviours among patients diagnosed with hypertension, a reduction in metabolic complications among frail patients receiving home enteral nutrition, a decline in hospital readmissions or emergency services utilization among patients >65 years of ages with at least one chronic disease, and, notably, a high level of effectiveness in detecting major cardiovascular events in patients with cardiac implantable electronic devices. Despite the effectiveness of interventions managed by FCNs, comprehensive information and research on the integration of this role within the PC setting are still lacking in the Italian healthcare system.

Adrenomedullin (ADM) is a peptide hormone produced primarily in the adrenal glands, playing a crucial role in various physiological processes. As well as improving vascular integrity and decreasing vascular permeability, ADM acts as a vasodilator, positive inotrope, diuretic, natriuretic and bronchodilator, antagonizing angiotensin II by inhibiting aldosterone secretion. ADM also has antihypertrophic, anti-apoptotic, antifibrotic, antioxidant, angiogenic and immunoregulatory effects and antimicrobial properties. ADM expression is upregulated by hypoxia, inflammation-inducing cytokines, viral or bacterial substances, strength of shear stress, and leakage of blood vessels. These pathological conditions are established during systemic inflammation that can result from infections, surgery, trauma/accidents or burns. The ability to rapidly identify infections and the prognostic, predictive power makes it a valuable tool in severe viral and bacterial infections burdened by high incidence and mortality. This review sheds light on the pathophysiological processes that in severe viral or bacterial infections cause endothelitis up to the development of organ damage, the resulting increase in ADM levels dosed through its more stable peptide mid-regional proadrenomedullin (MR-proADM), the most significant studies that attest to its diagnostic and prognostic accuracy in highlighting the severity of viral or bacterial infections and appropriate therapeutic insights.

Coastal and underwater archaeological sites pose significant challenges in terms of investigation, conservation, valorisation, and management. These sites are often at risk due to climate change and various human-made impacts such as urban expansion, maritime pollution, and natural deterioration. However, advances in remote sensing (RS) and Earth observation (EO) technologies applied to cultural heritage (CH) sites have led to the development of various techniques for underwater cultural heritage (UCH) exploration. The aim of this work was the evaluation of an integrated methodological approach using ultra-high-resolution (UHR) bathymetric data to aid in the identification and interpretation of submerged archaeological contexts. The study focused on a selected area of the submerged Archaeological Park of Baia (Campi Flegrei, south Italy) as a test site. The study highlighted the potential of an approach based on UHR digital bathymetric model (DBM) derivatives and the use of machine learning and statistical techniques to automatically extract and discriminate features of archaeological interest from other components of the seabed substrate. The results achieved accuracy rates of around 90% and created a georeferenced vector map similar to that usually drawn by hand by archaeologists.

Sepsis, a critical condition marked by systemic inflammation, profoundly impacts both innate and adaptive immunity, often resulting in lymphopenia. This immune alteration can spare regulatory T cells (Tregs) but significantly affects other lymphocyte subsets, leading to diminished effector functions, altered cytokine profiles, and metabolic changes. The complexity of sepsis stems not only from its pathophysiology but also from the heterogeneity of patient responses, posing significant challenges in developing universally effective therapies. This review emphasizes the importance of phenotyping in sepsis to enhance patient-specific diagnostic and therapeutic strategies. Phenotyping immune cells, which categorizes patients based on clinical and immunological characteristics, is pivotal for tailoring treatment approaches. Flow cytometry emerges as a crucial tool in this endeavor, offering rapid, low cost and detailed analysis of immune cell populations and their functional states. Indeed, this technology facilitates the understanding of immune dysfunctions in sepsis and contributes to the identification of novel biomarkers. Our review underscores the potential of integrating flow cytometry with omics data, machine learning and clinical observations to refine sepsis management, highlighting the shift towards personalized medicine in critical care. This approach could lead to more precise interventions, improving outcomes in this heterogeneously affected patient population.

Physical activity in the form of “active breaks” can be combined with academic instruction in primary school. However, few studies have examined the feasibility of conducting active breaks in secondary school. To address this gap, we conducted focus groups (FGs) regarding the implementation of an active breaks (ABs) protocol with 20 teachers and 10 secondary school students. Barriers/facilitators toward the implementation of ABs were classified using grounded theory inductive methods framed by the socio-ecological model. Individual-level factors were instrumental for both teachers and students. Teachers highlighted personal fears and concerns regarding using ABs, while students reported fears related to peer behaviour during the activity. Both teachers and students agreed that ABs can improve cognitive skills and time-on-task behaviour. Teachers articulated concerns related to student behaviour during ABs including possible social exclusion and injury. Students felt that ABs might affect classroom management and interfere with maintaining students’ academic focus. Teachers underscored that ABs required social support from the administration and colleagues. Students felt that ABs could support teachers’ instructional focus and provide them with an energy respite. Collectively, the FGs suggested that environmental limitations could hinder the implementation of ABs. Involving teacher and student feedback during the codesign phase can rationally inform the design of school-based ABs.

Tuberculosis (TB) remains one of the leading causes of morbidity and mortality worldwide and pulmonary TB (PTB) is the main variant responsible for fueling transmission of the infection. Effective treatment of drug-susceptible (DS) TB is crucial to avoid the emergence of Mycobacterium tuberculosis-resistant strains. In this narrative review, through a fictional suggestive case of DS PTB, we guide the reader in a step-by-step commentary to provide an updated review of current evidence in the management of TB, from diagnosis to post-treatment follow-up. World Health Organization and Centre for Diseases Control (CDC) guidelines for TB, as well as the updated literature, were used to support this manuscript.

Hyperextended rift systems are characterized by extreme crustal thinning and mantle exhumation associated with extensional detachment faults. These faults cut through thinned continental crust, reaching the underlying mantle and allowing for seawater to infiltrate and react with the crustal and mantle rocks. Hydrothermal fluid systems linked to detachment faults result in fluid–rock reactions occurring along the detachments, resulting in the breakdown and alteration of minerals, loss of elements and strain weakening in both mantle and crustal rocks. We present new geological observations and geochemical data from the modern Iberia and fossil Alpine Tethys Ocean Continent Transition and the West Pyrenean Mauléon hyperextended rift basin. We show evidence for a km-scale fluid flow along detachment faults and discuss the conditions under which fluid flow and mass transfer occurred. Convective fluid systems are of major importance for mass transfer between the mantle, crustal and marine reservoirs. We identified gains in Si, Mg, Fe, Mn, Ca, Ni, Cr and V along extensional detachment faults that we relate to channelized, hydrothermal crust- and mantle-reacted fluid systems migrating along detachments in the hyperextended continental crust. The observation that fault rocks of extensional detachment and syn-extensional sedimentary rocks are enriched in mantle-derived elements such as Cr, Ni and V enables us to define the pathways of fluids, as well as to estimate their age relative to detachment faulting and sedimentation. Because all three examples show a similar mass transport of elements along detachment systems at km-scale, we conclude that these examples are linked to convective fluid systems that may affect the thermal state of the lithosphere, as well as the rheology and chemistry of rocks in hyperextended systems, and may have implications for ore mineral exploration in hyperextended rift systems.

This study investigated the incidence, mortality, and 5-year survival rates of testicular cancers diagnosed in a northern Italian province, which were eventually associated with previous or subsequent extratesticular neoplasms. Cases from 1996 to 2020 were examined by age and histotype (seminoma vs. non-seminoma). The standardized incidence rate was calculated using the European population, and the annual percent change (APC) was reported. The five-year relative survival was estimated using the Pohar Perme method. The association with the second neoplasm was also evaluated. In our study, 385 patients with testicular cancer were included, most of whom were aged between 30 and 40 years. The non-seminoma and seminoma groups accounted for 44% and 18% of younger adults, respectively. The incidence rate increased during the study period (APC 1.6*); however, it increased in seminomas (APC 2.3*) but not in non-seminomas (APC −0.1). Conversely, the mortality rate remained constantly low either overall or in each of the two groups. The overall 5-year survival rate of testicular cancer patients was 95% (99% and 88% for seminomas and non-seminomas, respectively). Primary extratesticular tumors were documented in 37 cases, 18 after and 19 before the testicular cancer diagnosis. Our study confirms that the increased incidence and excellent survival rate are the prerogative of seminomas.

Amyloidosis is a rare disease that is often seen in conjunction with multiple myeloma (MM). Its damage varies depending on the anatomical site affected; however, it is believed that many cases of amyloidosis are misrecognized due to the fact that its signs and symptoms are nonspecific. Joint amyloidosis, in particular, may be confused with degenerative or autoimmune diseases. When it is associated with MM, it can significantly precede the diagnosis of the latter. We describe a case report of a woman of Nigerian heritage diagnosed with MM with widespread joint manifestations compatible with a diagnosis of amyloidosis, which had preceded the diagnosis of MM and benefited from MM treatment. Faced with the suspicion of amyloidosis, if confirmed, this can be used to anticipate the diagnosis of MM, and at a more advanced stage, it can benefit from the treatment of the MM.

Background: With global changes in markets and government regulations, most organizations, which previously opted for offshore production, began to return to their country of origin—a process called reshoring. The new strategies for locating units and business models, which involve decisions to outsource production and organizational capacity, now face a new context of Industry 4.0, a market for technologies with the potential to transform entire supply chains in addition to directly influencing organizational aspects with innovative solutions and resource efficiency. Methods: This systematic literature review (SLR) found 43 articles relating to reshoring and Industry 4.0; there was an increase in publications in the last few years. We divided the studies according to 9 aspects: market uncertainties and labor supply; support for managerial decision making; competitive priorities analysis (costs and efficiency); business strategies; information knowledge and collaboration management; political, social and economic changes; risk security and privacy management; supply chain management; and investment market and private equity funds. Results: This article presents a content analysis of articles in the field of administration, focusing on location strategies, technology adoption, and organizational changes. The study identifies key aspects such as research quality, publication trends, regional focus, and strategy topics. The findings emphasize the importance of research objectives and problem-solving for managerial decision-making and highlight the need for further exploration in the literature. Conclusions: Reshoring, Industry 4.0, and location strategies are relevant topics for global management. The supply chain plays a role in decision-making for location and technology adoption, suggesting the need for more research in supply chain management.

Background: Since limited data are available, we aimed to compare the efficacy and durability of dolutegravir and darunavir in advanced naïve patients. Methods: Retrospective multicenter study including AIDS- or late-presenting (def. CD4 ≤ 200/µL) HIV-infected patients starting dolutegravir or ritonavir/cobicistat-boosted darunavir+2NRTIs. Patients were followed from the date of first-line therapy initiation (baseline, BL) to the discontinuation of darunavir or dolutegravir, or for a maximum of 36 months of follow-up. Results: Overall 308 patients (79.2% males, median age 43 years, 40.3% AIDS-presenters, median CD4 66 cells/µL) were enrolled; 181 (58.8%) and 127 (41.2%) were treated with dolutegravir and darunavir, respectively. Incidence of treatment discontinuation (TD), virological failure (VF, defined as a single HIV-RNA > 1000 cp/mL or two consecutive HIV-RNA > 50 cp/mL after 6 months of therapy or after virological suppression had been achieved), treatment failure (the first of TD or VF), and optimal immunological recovery (defined as CD4 ≥ 500/µL + CD4 ≥ 30% + CD4/CD8 ≥ 1) were 21.9, 5.2, 25.6 and 1.4 per 100 person-years of follow-up, respectively, without significant differences between dolutegravir and darunavir (p > 0.05 for all outcomes). However, a higher estimated probability of TD for central nervous system (CNS) toxicity (at 36 months: 11.7% vs. 0%, p = 0.002) was observed for dolutegravir, whereas darunavir showed a higher probability of TD for simplification (at 36 months: 21.3% vs. 5.7%, p = 0.046). Conclusions: Dolutegravir and darunavir showed similar efficacy in AIDS- and late-presenting patients. A higher risk of TD due to CNS toxicity was observed with dolutegravir, and a higher probability of treatment simplification with darunavir.

Climate change and compostinS1g methods have an important junction on the phenological and ripening grapevine phases. Moreover, the optimization of these composting methods in closed-loop corporate chains can skillfully address the waste problem (pomace, stalks, and pruning residues) in viticultural areas. Owing to the ongoing global warming, in many wine-growing regions, there has been unbalanced ripening, with tricky harvests. Excessive temperatures in fact impoverish the anthocyanin amount of the must while the serious water deficits do not allow a correct development of the berry, stopping its growth processes. This experiment was created to improve the soil management and the quality of the grapes, through the application of a new land conditioner (Zeowine) to the soil, derived from the compost processes of industrial wine, waste, and zeolite. Three treatments on a Sangiovese vineyard were conducted: Zeowine (ZW) (30 tons per ha), Zeolite (Z) (10 tons per ha), and Compost (C) (20 tons per ha). During the two seasons (2021–2022), measurements were made of single-leaf gas exchange and leaf midday water potential, as well as chlorophyll fluorescence. In addition, the parameters of plant yield, yeast assimilable nitrogen, technological maturity, fractionation of anthocyanins (Cyanidin-3-glucoside, Delphinidin-3-glucoside, Malvidin-3-acetylglucoside, Malvidin-3-cumarylglucoside, Malvidin-3-glucoside, Peonidin-3-acetylglucoside, Peonidin-3-cumarylglucoside, Peonidin-3-glucoside, and Petunidin-3-glucoside), Caffeic Acid, Coumaric Acid, Gallic Acid, Ferulic Acid, Kaempferol-3-O-glucoside, Quercetin-3-O-rutinoside, Quercetin-3-O-glucoside, Quercetin-3-O-galactoside, and Quercetin-3-O-glucuronide were analyzed. The Zeowine and zeolite showed less negative water potential, higher photosynthesis, and lower leaf temperature. Furthermore, they showed higher levels of anthocyanin accumulation and a lower level of quercetin. Finally, the interaction of the beneficial results of Zeowine (soil and grapevines) was evidenced by the embellishment of the nutritional and water efficiency, the minimizing of the need for fertilizers, the closure of the production cycle of waste material from the supply chain, and the improvement of the quality of the wines.

Regular physical activity (PA) is protective and reduces disease burden but remains a challenge for pregnant women (PW). According to the World Health Organization (WHO) guidelines, PW without contraindications should practice 150 min of moderate PA per week. Nonetheless, PA levels are concerningly low among PW. The aim of this study was to investigate PW’s and midwives’ perceptions regarding PA and recommended guidelines, and use this information to inform future health promotion strategies. We recruited 10 PW and 10 midwives to participate in online focus groups conducted between July 2020 and April 2021. Focus group probes and data analysis were guided by the COM–B (capability, opportunity, motivation–behaviour) framework. The majority of the sample had already practised PA, recognized the importance of PA during pregnancy, and considered the WHO guidelines reasonable. Notwithstanding, PW wanted more specific instruction on PA and desired opportunities to practice. Additional barriers reported by PW included low self-efficacy and lack of motivation. Midwives considered the lack of specific knowledge and confidence in managing PA as the main obstacles. The current findings suggest that PW and midwives need specific training in PA to overcome both psychological and physical barriers. Midwives play a vital role in educating and encouraging PA among PW.

This paper focuses on the potential of an integrated approach using aerial LiDAR, aerial and terrestrial photogrammetry, terrestrial laser scanning, and archaeological survey to detect the presence and configuration of lost medieval settlements under canopy. This approach was applied to the site of Altanum (Calabria, Italy), on the hill of Sant’Eusebio, completely covered by vegetation. Altanum was a large fortified settlement characterised by a long occupation, especially during the Byzantine and Norman-Swabian periods. The activity began by carrying out a LiDAR survey of the whole hill. The acquired LiDAR data were processed and filtered in order to obtain a DFM (Digital Feature Model) useful for the identification of features of archaeological interest. Several enhancement techniques were performed on DFM to increase the visibility of archaeological features. The features thus identified were subsequently surveyed through the use of terrestrial and aerial photogrammetry integrated with laser scanning to document the visible buildings. The most significant result of the study was to create a single GIS platform with the integration of all data in order to delineate the whole settlement layout, as well as to produce 2D and 3D datasets useful for the for knowledge and protection of the identified remains.

Background: The COVID-19 had a strong impact on the physical and general well-being of the youngest. In Italy, citizens were forced to change their habits, especially during the national lockdown, causing increased levels of sedentary and unhealthy behaviors. “Come te la passi?” was a cross-sectional study aimed at investigating changes in the physical activity levels (PA) and well-being of children and adolescents in the City of Bologna. Methods: An anonymous survey was administered to parents/guardians of children and adolescents aged 6–18 years. Results: 1134 questionnaires were collected during June 2021; 457 (40.3%) were females, and the mean age was 13.0 ± 3.4 years. Regarding the general well-being perception, 61.3% of the participants reported concerns about the future, 46.3% reported sleep difficulties, and 72.8% reported experiencing attention difficulty, with higher percentages among adolescents. Considering the PA frequency, an overall reduction was found, with the percentage of those who rarely did PA and those who frequently did PA both increasing. No gender differences were found. Conclusions: Our findings suggest that the current pandemic has strongly impacted the well-being of children and adolescents. It appears to have primarily affected adolescents, with a significant reduction in PA levels, even after the end of the national lockdown.

This paper reports on a compact, portable, and selective chemical sensor for hazardous vapors at trace levels, which is under development and validation within the EU project H2020 “RISEN”. Starting from the prototype developed for a previous EU project, here, we implemented an updated two-stage purge and trap vapor pre-concentration system, a more compact MEMS- based fast gas-chromatographic separation module (Compact-GC), a new miniaturized quartz-enhanced photoacoustic spectroscopy (QEPAS) detector, and a new compact laser source. The system provides two-dimensional selectivity combining GC retention time and QEPAS spectral information and was specifically designed to be rugged, portable, suitable for on-site analysis of a crime scene, with accurate response in few minutes and in the presence of strong chemical background. The main upgrades of the sensor components and functional modules will be presented in detail, and test results with VOCs, simulants of hazardous chemical agents, and drug precursors will be reported and discussed.

This paper deals with a UAV LiDAR methodological approach for the identification and extraction of archaeological features under canopy in hilly Mediterranean environments, characterized by complex topography and strong erosion. The presence of trees and undergrowth makes the reconnaissance of archaeological features and remains very difficult, while the erosion, increased by slope, tends to adversely affect the microtopographical features of potential archaeological interest, thus making them hardly identifiable. For the purpose of our investigations, a UAV LiDAR survey has been carried out at Perticara (located in Basilicata southern Italy), an abandoned medieval village located in a geologically fragile area, characterized by complex topography, strong erosion, and a dense forest cover. All of these characteristics pose serious challenge issues and make this site particularly significant and attractive for the setting and testing of an optimal LiDAR-based approach to analyze hilly forested regions searching for subtle archaeological features. The LiDAR based investigations were based on three steps: (i) field data acquisition and data pre-processing, (ii) data post-processing, and (iii) semi-automatic feature extraction method based on machine learning and local statistics. The results obtained from the LiDAR based analyses (successfully confirmed by the field survey) made it possible to identify the lost medieval village that represents an emblematic case of settlement abandoned during the crisis of the late Middle Ages that affected most regions in southern Italy.

We reassess the architecture and tectonic history of the Western Alps based on recent knowledge developed at rifted margins. First, we replace the main Alpine units of our study area into a synthetic rifted margin template based on diagnostic petrologic, stratigraphic, and structural criteria. We find that some units previously attributed to the internal part of the thick-crusted Briançonnais domain may rather derive from the thin-crusted Prepiemonte hyperextended domain. We assert that the Briançonnais and Prepiemonte domains were separated by a mega-fault scarp. Second, we revisit the Paleogeography of the Alpine Tethys, suggesting that the Briançonnais was a ribbon of little thinned continental crust between two overstepping en-échelon rift basins, namely the Valais domain to the northwest and the Piemonte domain to the southeast. We affirm that this uneven-margin architecture can explain most of the Western Alps’ complexity. In our kinematic model, convergence between Adria and Europe was mainly accommodated by strike-slip movements in the Western Alps until the late Eocene. Orogeny began with the reactivation of the mega-fault scarp between the Briançonnais and Prepiemonte domains, which we name Prepiemonte Basal Thrust. Once hard collision started, the main shortening stepped inboard into the Valais/Subbriançonnais domain along the Penninic Basal Thrust.

Physical activity has been indicated as a potential strategy to counteract osteoporosis (OP). This study of post-menopausal women with osteoporotic vertebral fractures investigated the effect of an adapted physical activity (APA) program on two serum bone turnover biomarkers (Bone Alkaline Phosphatase, B-ALP and C-terminal telopeptide of type 1 collagen, CTX-1), functional capacity (6-Minutes Walking Test, 6MWT), and risk and fear of falls (Tinetti and Falls Efficacy scale). The APA group (n = 12) performed a 1-h group session twice per week for 6 months whereas the control group (n = 9) was asked to maintain their current lifestyle. The exercise program did not affect the serum concentrations of B-ALP and CTX-1 biomarkers measured at the baseline and after 6 months in women of the APA group. Moreover, at the end of intervention no significant differences in serum concentrations for either biomarker was observed between the two study groups. Interestingly, when compared to the control group, women in the APA group showed significant improvement in the functional capacity measures by 6MWT (p = 0.037) and a decrease of the risk and fear of falls as indicated by the Tinetti test (p = 0.043). Based on these findings, exercise could provide new perspectives for the care and management of OP.

Background: Idiopathic polyhydramnios is a controversial clinical condition, as data on perinatal outcomes are conflicting and vary depending on the severity of the condition. The aim of the present study was to compare obstetric and neonatal outcomes between pregnant women with mild idiopathic polyhydramnios and a control population. Methods: A retrospective cohort study was performed at a single university hospital comparing the obstetrics and neonatal outcomes of pregnancies with mild idiopathic polyhydramnios (n = 109) and control pregnancies (n = 2550). Results: Cesarean section (CS) was significantly increased in the group with polyhydramnios compared to controls (46% vs. 32%, respectively, p = 0.047) due to a higher rate of emergency CS in the polyhydramnios group (p = 0.041) because of abnormal cardiotocography (7.3% vs. 2.9%; p = 0.018) or labor dystocia (8.2% vs. 2.9%; p = 0.006). No statistically significant difference was found in the Apgar score, in the rate of neonatal hypoxia, or in the incidence of macrosomia between groups. In four cases, additional diagnoses of anomalies were made after birth, with a rate of 3.2%, which is comparable to the general population. Conclusion: Besides an increased risk of CS, patients with mild idiopathic polyhydramnios should be reassured regarding maternal and feto-neonatal outcomes. The management of pregnancies with stable mild idiopathic polyhydramnios should not differ from uncomplicated pregnancies, except for the need for increased labor surveillance.

The aim of this study was to examine the incidence and mortality trends for tumors and cardiovascular disease (CVD) in a province of northern Italy. The study included kidney cancers recorded in the period 1996–2020, divided by sex, age, year of incidence and years from diagnosis. The standardized incidence rate was calculated using the European population, and the Annual Percent Change (APC) was reported. In total, 2331 patients with kidney cancers were identified, mainly males (1504 cases) aged 60–79 years (1240 cases). There were 1257 deaths; there were no differences according sex but there were differences according to age (12.1% among younger adults and 80.4% among 80+). The incidence rate increased in males between 1996 and 2011 (APC = 2.3), while the mortality rate decreased in both males (APC = −3.3%) and females (APC = −4.5%). Comparing the same periods, kidney cancer-specific mortality decreased from 81.8% to 43.7%, while in the same period there was an increasing trend for CVD mortality. Moreover, the risk of CVD mortality increased as we moved away from the diagnosis (from 6.2% to 27.5%, p < 0.01). The same trend was observed for other causes of death (from 12.6% to 32.1%, p < 0.01). Thus, a multidisciplinary approach seems necessary during the follow-up and treatments of patients with kidney cancer.

The social acceptability of wind farms has been researched for several decades now, with the first research tracing back to the 1980s. This paper aims to deliver a literature review within the structural framework proposed by the paper of variables influencing the acceptability of wind farms. The large amount of research published on the social acceptability of wind farms requires an effort to identify and categorise variables to deliver a holistic understanding of opposition and support to wind energy. We classify the variables into three main categories: first, ‘psychological variables’, including perceived benefits and costs, emotions, and attitudes; second, ‘contextual variables’, including community energy schemes and media influence; and finally, third, ‘personal resources’, including income or wealth, place of residence, and relevant knowledge in relation to the wind farm. In agreement with other scholars, we argue that NIMBYism (not in my backyard) is an outdated and simplistic explanation for opposition to wind farms. Instead, we provide a theoretical framework to explain the acceptability of wind energy and show how these variables might influence both acceptance and opposition.

Since prostate cancer is the most commonly diagnosed malignancy in men, the theranostic approach has become very attractive since the discovery of urea-based PSMA inhibitors. Different molecules have been synthesized starting from the Glu-urea-Lys scaffold as the pharmacophore and then optimizing the linker and the chelate to improve functional characteristics. This article aimed to highlight the quality aspects, which could have an impact on clinical practice, describing the development of an Investigational Medicinal Product Dossier (IMPD) for clinical trials with [177Lu]Lu-PSMA-I&T in prostate cancer and other solid tumors expressing PSMA. The results highlighted some important quality issues of the final preparation: radiolabeling of PSMA-I&T with lutetium-177 needs a considerably longer time compared with the radiolabeling of the well-known [177Lu]Lu-PSMA-617. When the final product was formulated in saline, the stability of [177Lu]Lu-PSMA-I&T was reduced by radiolysis, showing a decrease in radiochemical purity (

The increasing availability of multiplatform, multiband, very-high-resolution (VHR) satellite synthetic aperture radar (SAR) data has attracted the attention of a growing number of scientists and archeologists. In particular, over the last two decades, archeological research has benefited from SAR development mainly due to its unique ability to acquire scenes both at night and during the day under all weather conditions, its penetration capability, and the provided polarimetric and interferometric information. This paper explored the potential of a novel method (nonlocal (NL)-SAR) using TerraSAR-X (TSX) and Constellation of Small Satellites for Mediterranean Basin Observation (COSMO)-SkyMed (CSK) data to detect buried archeological remains in steep, rugged terrain. In this investigation, two test sites were selected in southern Tunisia, home to some of the most valuable and well-preserved limes from the Roman Empire. To enhance the subtle signals linked to archeological features, the speckle noise introduced into SAR data by the environment and SAR system must be mitigated. Accordingly, the NL-SAR method was applied to SAR data pertaining to these two significant test sites. Overall, the investigation (i) revealed a fortified settlement from the Roman Empire and (ii) identified an unknown urban area abandoned during this period via a field survey, thus successfully confirming the capability of SAR data to reveal unknown, concealed archeological sites, even in areas with a complex topography.

The first properties of histamine (HA) that were elucidated were vasodilation and contraction of smooth muscles in the gut after stimulating gastric acid secretion and constriction of the bronchial area during anaphylaxis [...]

Illegal archaeological excavations, generally denoted as looting, is one of the most important damage factors to cultural heritage, as it upsets the human occupation stratigraphy of sites of archaeological interest. Looting identification and monitoring are not an easy task. A consolidated instrument used for the detection of archaeological features in general, and more specifically for the study of looting is remote sensing. Nevertheless, passive optical remote sensing is quite ineffective in dense vegetated areas. For these type of areas, in recent decades, LiDAR data and its derivatives have become an essential tool as they provide fundamental information that can be critical not only for the identification of unknown archaeological remains, but also for monitoring issues. Actually, LiDAR can suitably reveal grave robber devastation, even if, surprisingly, up today LiDAR has been generally unused for the identification of looting phenomenon. Consequently, this paper deals with an approach devised ad hoc for LiDAR data to detect looting. With this aim, some spatial visualization techniques and the geomorphon automatic landform extraction were exploited to enhance and extract features linked to the grave robber devastation. For this paper, the Etruscan site of San Giovenale (Northern Lazio, Italy) was selected as a test area as it is densely vegetated and was deeply plundered throughout the 20th century. Exploiting the LiDAR penetration capability, the prediction ability of the devised approach is highly satisfactory with a high rate of success, varying from 85–95%.

The World Health Organization’s Model List of Essential Medicines for Children (EMLc) presents a list of the most efficacious, safe, and cost-effective medicines for priority conditions, intended for use in children up to 12 years of age. However, gaps in global availability and use of age-appropriate formulations of medicines for children still exist. To address these shortcomings, a comprehensive analysis of the appropriateness of formulations of essential medicines for children is being undertaken through the Global Accelerator for Paediatric Formulations (GAP-f) network, a WHO network launched in 2020 to respond to the paediatric treatment gap. This article describes the development and application of a paediatric Quality Target Product Profile (pQTPP) tool by WHO, to retrospectively evaluate the paediatric age-appropriateness of formulations on the EMLc and identify potential formulation gaps, to inform the review of the EMLc in 2023. A combination of paediatric-centric and global health-focused attributes and targets were defined, taking into consideration regulatory agency paediatric development guidelines and literature sources, and a qualitative scoring system was developed and tested. Example evaluations of paracetamol and clofazimine are provided, illustrating the tool’s use. The assessment of EMLc formulations is ongoing and shortcomings and gaps in EMLc formulations have already been identified. The pQTTP tool may also be applied to national lists and prospectively when designing new paediatric formulations.

A3 adenosine receptor (A3AR) agonists have emerged as potent relievers of neuropathic pain by a T cell-mediated production of IL-10. The H4 histamine receptor (H4R), also implicated in pain modulation, is expressed on T cells playing a preeminent role in its activation and release of IL-10. To improve the therapeutic opportunities, this study aimed to verify the hypothesis of a possible cross-talk between A3AR and H4R in the resolution of neuropathic pain. In the mouse model of Chronic Constriction Injury (CCI), the acute intraperitoneal co-administration of the A3AR agonist IB-MECA (0.5 mg/kg) and the H4R agonist VUF 8430 (10 mg/kg), were additive in counteracting mechano-allodynia increasing IL-10 plasma levels. In H4R−/− mice, IB-MECA activity was reduced, lower pain relief and lower modulation of plasma IL-1β, TNF-α, IL-6 and IL-10 were shown. The complete anti-allodynia effect of IB-MECA in H4R−/− mice was restored after intravenous administration of CD4+ T cells obtained from naïve wild type mice. In conclusion, a role of the histaminergic system in the mechanism of A3AR-mediated neuropathic pain relief was suggested highlighting the driving force evoked by CD4+ T cells throughout IL-10 up-regulation.

Glaucoma is a multifactorial neuropathy characterized by increased intraocular pressure (IOP), and it is the second leading cause of blindness worldwide after cataracts. Glaucoma combines a group of optic neuropathies characterized by the progressive degeneration of retinal ganglionic cells (RGCs). Increased IOP and short-term IOP fluctuation are two of the most critical risk factors in glaucoma progression. Histamine is a well-characterized neuromodulator that follows a circadian rhythm, regulates IOP and modulates retinal circuits and vision. This review summarizes findings from animal models on the role of histamine and its receptors in the eye, focusing on the effects of histamine H3 receptor antagonists for the future treatment of glaucomatous patients.