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Gentiana lutea L. subsp. aurantiaca M. Lainz is a plant endemic to the north-western mountainous areas of the Iberian Peninsula. Its roots are widely used mainly because of the high content of bitter compounds. The occurrence of these valuable bitter compounds in the roots is rather inhomogeneous, resulting in fluctuating root quality. Methanolic extracts obtained from different parts and tissues of wild and cultivated gentian, in and out of its natural environment, were analysed using HPLC chromatography to investigate the variation in the concentration of amarogentin, gentiopicroside, sweroside and swertiamarin. The distribution patterns of these compounds in the different analysed fractions showed that the concentration of bitter compounds varies significantly. Amarogentin is much more highly concentrated in the secondary roots, and all of the analysed compounds were found in a significantly higher content in the root cortex than in the vascular tissues. Roots cultivated in the natural habitat showed much higher concentrations in amarogentin and more biomass, while in those cultivated out of the natural environment, sweroside concentration was higher. These results allow us to understand that, when cultivated, the variability in the concentration of the different bitter compounds is linked with the edaphoclimatic conditions, but more importantly that it is linked with the dominating kind of tissues and the root system structure, especially when analysing the content of amarogentin and sweroside. The selection of plants with an optimal root system structure for breeding may increase the yield in bitter compounds and contribute to developing the commercial cultivation of this protected plant.

Using waste biomass is considered one of the ways to reduce climate change. Arboreal waste biomass from pine, eucalyptus, and particularly invasive plants (Acacias) would make it possible to transform this natural resource, but needs to be adjusted to current and innovative technologies. The production of high fixed carbon biochar with this biomass would improve not only environmental aspects, but also the use of currently not susceptible materials for other types of exploitation. The objective of this study is to develop biochar from three different waste biomass materials and compare their parameters with those of the original biomass. Thermochemical conversion processes were used in a simple double chamber reactor developed for this study. Temperatures between 400 and 500 °C during 280 min were achieved and allowed to transform the initial biomass in a biochar with a high content of fixed carbon. By comparing the original biomass with the final biochar through tests of humidity, density, calorific values, fixed carbon, and cationic and elemental analysis, an increase in the parameters was confirmed. Fixed carbon of 70%, 77%, and 71% with pine, acacia, and eucalyptus biomass have been obtained, respectively, with yields between 30% and 40%. The results are favorable, particularly with acacia invasive plants, and could help in their difficult silviculture management.

We studied two Eucalyptus globulus salvage clearcuts—after a wildfire and a Gonipterus attack—and a strong thinning on a dense Pinus pinaster stand which was regenerated 20 years after a wildfire and also affected by a Matsococcus pest. Biomass harvesting was performed using the feller-bundler Fixteri FX-15a, which was time-studied during several weeks using GNSS combined with an automatic weight/time registration system (WNexus-2®). Detailed in situ time studies were applied during shorter periods as well. The productivity equations found as main explanative factors for the salvage clearcuts the unit weight per tree and the felling reason (wildfire vs. pest); for the thinning, besides the unit weight, the percentage of extracted basal area explained the productivity. Biomass collection did not allow an economic positive balance: in the Gonipterus-affected plantation the cost was reduced to 125 €·ha−1, reaching a zero balance for unit weights greater than 33 kg (dry matter) per tree; the restoration cost in the pine stand was 265 €·ha−1, much lower than the cost without biomass harvesting. In the burned eucalypt, the zero cost would be achieved for a dry unit weight much greater than the observed values. Although Fixteri performance and utilization were remarkable, recommendations about possible improvements of its design and operation were concluded from the detailed time studies.

Introduction: Mucopolysaccharidosis type I (MPS I) is a lysosomal storage disease present in 1:100,000 newborns. Variants in the IDUA (alpha-L-iduronidase) gene decrease the enzyme activity for glycosaminoglycans metabolism. MPS I patients exhibit clinical manifestations that fall on the Hurler, Hurler–Scheie, and Scheie syndrome spectrum. Case presentation: We present a male Mexican patient with respiratory exacerbations requiring recurrent hospitalizations. He showed macrocephaly, coarse facies, hepatomegaly, umbilical hernia, and dorsal kyphosis. The sequencing of the IDUA gene revealed the following genotype: c.46_57del12/c.1205G>A. He received combined therapy with hematopoietic stem cell transplantation and enzyme replacement. Mexican case reports were analyzed to estimate the prevalence of the associated genetic variants. Conclusion: Despite the challenges of managing this rare disease in Mexico, our patient benefited from the combined therapy. The discrete clinical manifestations and prompt evaluation by a geneticist were crucial in establishing a diagnosis, enabling an early intervention by a multidisciplinary team. The combination of ERT before and after HSCT provided health benefits to our patient.

A re-immunization programme has been tested to improve the protective response elicited in sole by a previously developed BEI-inactivated betanodavirus vaccine. The vaccine was prepared using a reassortant RGNNV/SJNNV strain which is highly pathogenic for sole, and vaccination assays were performed by intraperitoneal injection. Experimental design included a prime- and a booster-vaccination group, which consisted of individuals that received a second vaccine injection at 30 days post vaccination), and their respective controls. A month after prime/booster vaccination, fish were challenged by intramuscular injection with the homologous NNV strain. Samples were collected at different times post vaccination and post challenge to assess the immune response and viral replication. Booster dose enhanced the protection against NNV infection because a significant increase in survival was recorded when compared with prime-vaccinated individuals (relative percent survival 77 vs. 55). In addition, a clear decrease in viral replication in the brain of challenged sole was observed. During the immune induction period, no differences in IgM production were observed between prime- and booster-vaccinated fish, and the expression of the antigen presenting cells (APC)-related molecule MHC class II antigen was the only differential stimulation recorded in the re-immunized individuals. However, a significant upregulation of mhcII and the lymphocytes T helper (Th) marker cd4 was observed after the challenge in the booster-vaccinated group, suggesting these cells play a role in the protection conferred by the booster injection. In addition, after viral infection, re-immunized fish showed specific and neutralizing antibody production and overexpression of other immune-related genes putatively involved in the control of NNV replication.

The beetle Xylotrechus arvicola is a destructive pest in vineyards (Vitis vinifera) in the main wine-producing areas of the Iberian Peninsula. X. arvicola larvae bore into the grapevine wood-making galleries, thus damaging the plant both directly and indirectly; the latter through the proliferation of wood fungi, which can invade the inside of the plant, decreasing the quality and quantity of its production. The susceptibility of X. arvicola larvae to five coleopteran toxic Cry proteins (Cry1B, Cry1I, Cry3A, Cry7A, and Cry23/37) was evaluated under laboratory conditions in order to deepen the knowledge of the effect of these proteins on this insect throughout its biological development. Cry7Ab and Cry1Ba were the most effective in controlling X. arvicola larvae due to the significant reduction in larvae survival (32.9 and 25.9 days, respectively), and by causing serious alterations in the larvae during the remaining months of their development. The developmental stage of the prepupal and pupal stages was not affected by the previous ingestion of Cry proteins. The Cry proteins tested could be applied to control X. arvicola larvae since they were able to kill them and cause serious alterations in the larvae during the remaining months of development that followed. The data presented suggest that these Cry proteins can be used as bioinsecticides against the larvae of this insect, applying them only at the moment when the larvae hatch from the egg outside the grapevine wood (this would only be useful and justified if the economic threshold is exceeded) in order to avoid the rapid evolution of resistance against these toxins since not all of the larvae were killed and thus increase vine wood protection.

The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the volatile organic compound chamber (VOC chamber), specifically designed to perform these experiments. The novel devices were tested using four Trichoderma strains against Fusarium oxysporum and Rhizoctonia solani. We demonstrate that VOC chambers provide higher sensitivity and selectivity between treatments and higher homogeneity of results than the traditional method. VOC chambers are also able to test both vented and non-vented conditions. We prove that ventilation plays a very important role regarding volatile interactions, up to the point that some growth-inhibitory effects observed in closed environments switch to promoting ones when tested in vented conditions. This promoting activity seems to be related to the accumulation of squalene by T. harzianum. The VOC chambers proved to be an easy, homogeneous, flexible, and repeatable method, able to better select microorganisms with high biocontrol activity and to guide the future identification of new bioactive VOCs and their role in microbial interactions.

This paper provides an overview of the last 40 years of use, and in many cases abuse, of the natural resources in Catalonia, a country that is representative of European countries in general, and especially those in the Mediterranean region. It analyses the use of natural resources made by mining, agriculture, livestock, logging, fishing, nature tourism, and energy production and consumption. This use results in an ecological footprint, i.e., the productive land and sea surface required to generate the consumed resources and absorb the resulting waste, which is about seven times the amount available, a very high number but very similar to other European countries. This overexploitation of natural resources has a huge impact on land and its different forms of cover, air, and water. For the last 25 years, forests and urban areas have each gained almost 3% more of the territory at the expense of agricultural land; those municipalities bordering the sea have increased their number of inhabitants and activity, and although they only occupy 6.7% of the total surface area, they account for 43.3% of the population; air quality has stabilized since the turn of the century, and there has been some improvement in the state of aquatic ecosystems, but still only 36% are in good condition, while the remainder have suffered morphological changes and different forms of nonpoint source pollution; meanwhile the biodiversity of flora and fauna remains still under threat. Environmental policies do not go far enough so there is a need for revision of the legislation related to environmental impact and the protection of natural areas, flora, and fauna. The promotion of environmental research must be accompanied by environmental education to foster a society which is more knowledgeable, has more control and influence over the decisions that deeply affect it. Indeed, nature conservation goes hand in hand with other social and economic challenges that require a more sustainable vision. Today’s problems with nature derive from the current economic model, which is environmentally unsustainable in that it does not take into account environmental impacts. Lastly, we propose a series of reasonable and feasible priority measures and actions related to each use made of the country’s natural resources, to the impacts they have had, and to their management, in the hope that these can contribute to improving the conservation and management of the environment and biodiversity and move towards sustainability.

Fungi from the genus Trichoderma have a worldwide distribution and are commonly found in agricultural lands. Further, it has been described as a non-virulent and symbiont microorganism that can contribute to minimize the pernicious effects of pathogens. In the present work we have isolated Trichoderma spp. from bark of grapevine in different orchards in order to determine the influence of fungicide application and vine age on Trichoderma diversity in plant. An opposite correlation between the number of fungicides sprayed per campaign and the diversity of Trichoderma spp. isolates was found. Moreover, the older are grapevine plants the higher is the diversity of Trichoderma spp. isolates. The different Trichoderma strains isolated were tested against Phaeoacremonium minimum, a grapevine trunk pathogen, to evaluate their biocontrol capacity. Three Trichoderma strains shown a significant capacity to control P. minimum and were selected as candidates to be used as biological control agents. In addition, a rapid and easy method for isolating Trichoderma spp. from grapevine plants has been developed, which allowed to determine that the reduction in the amount of pesticide use, together with the preservation of old vineyards, lead to healthier agroecosystems containing higher levels of beneficial microorganisms.

Today, environmental impact associated with pollution treatment is a matter of great concern. A method is proposed for evaluating environmental risk associated with Advanced Oxidation Processes (AOPs) applied to wastewater treatment. The method is based on the type of pollution (wastewater, solids, air or soil) and on materials and energy consumption. An Environmental Risk Index (E), constructed from numerical criteria provided, is presented for environmental comparison of processes and/or operations. The Operation Environmental Risk Index (EOi) for each of the unit operations involved in the process and the Aspects Environmental Risk Index (EAj) for process conditions were also estimated. Relative indexes were calculated to evaluate the risk of each operation (E/NOP) or aspect (E/NAS) involved in the process, and the percentage of the maximum achievable for each operation and aspect was found. A practical application of the method is presented for two AOPs: photo-Fenton and heterogeneous photocatalysis with suspended TiO2 in Solarbox. The results report the environmental risks associated with each process, so that AOPs tested and the operations involved with them can be compared.

Cyclic AMP acts as a secondary messenger involving different cellular functions in eukaryotes. Here, proteomic and transcriptomic profiling has been combined to identify novel early developmentally regulated proteins in eukaryote cells. These proteomic and transcriptomic experiments were performed in Dictyostelium discoideum given the unique advantages that this organism offers as a eukaryotic model for cell motility and as a nonmammalian model of human disease. By comparing whole-cell proteome analysis of developed (cAMP-pulsed) wild-type AX2 cells and an independent transcriptomic analysis of developed wild-type AX4 cells, our results show that up to 70% of the identified proteins overlap in the two independent studies. Among them, we have found 26 proteins previously related to cAMP signaling and identified 110 novel proteins involved in calcium signaling, adhesion, actin cytoskeleton, the ubiquitin-proteasome pathway, metabolism, and proteins that previously lacked any annotation. Our study validates previous findings, mostly for the canonical cAMP-pathway, and also generates further insight into the complexity of the transcriptomic changes during early development. This article also compares proteomic data between parental and cells lacking glkA, a GSK-3 kinase implicated in substrate adhesion and chemotaxis in Dictyostelium. This analysis reveals a set of proteins that show differences in expression in the two strains as well as overlapping protein level changes independent of GlkA.

Genetic improvement of coffee plants represents a great challenge for breeders. Conventional breeding takes a too long time for responding timely to market demands, climatic variations and new biological threads. The correlation of genetic markers with the plant phenotype and final product quality is usually poor. Additionally, the creation and use of genetically modified organisms (GMOs) are often legally restricted and rejected by customers that demand natural products. Therefore, we developed a non-targeted metabolomics approach to accelerate conventional breeding. Our main idea was to identify highly heritable metabolites in Coffea canephora seedlings, which are linked to coffee cup quality. We employed a maternal half-sibs approach to estimate the metabolites heritability in open-pollinated plants in both leaves and fruits at an early plant development stage. We evaluated the cup quality of roasted beans and correlated highly heritable metabolites with sensory quality traits of the coffee beverage. Our results provide new insights about the heritability of metabolites of C. canephora plants. Furthermore, we found strong correlations between highly heritable metabolites and sensory traits of coffee beverage. We revealed metabolites that serve as predictive metabolite markers at an early development stage of coffee plants. Informed decisions can be made on plants of six months old, compared to 3.5 to 5 years using conventional selection methods. The metabolome-wide association study (MWAS) drastically accelerates the selection of C. canephora plants with desirable characteristics and represents a novel approach for the focused breeding of crops.

The bean weevil, Acanthoscelides obtectus Say (Coleoptera: Chrysomelidae: Bruchinae), causes severe post-harvest losses in the common bean, Phaseolus vulgaris L. The control of this insect is still poor and involves the use of conventional insecticides. There is an increasing demand in the search for new active substances and products for pest control towards reduction of adverse effects on human health and the environment. The protection of grains with alternative products, such as essential oils, is a possible alternative to meet the needs described above. Therefore, this investigation evaluated the applications of basil, Ocimum basilicum, and citronella, Cymbopogon winterianus, essential oils for A. obtectus control. These essential oils significantly reduced the bean weight losses and the number of beans damaged by A. obtectus at higher doses than 60 or 120 μL/sample. The number of holes per bean did not differ between the doses of basil essential oil, not even at the dose of 60 μL, while it was higher at 120 μL, probably due to a lower capacity of movement of the insects treated with this dose and/or the oil’s direct or indirect effects on the insects. Basil and citronella oils exhibited similar patterns of insecticidal activity over the insect, both directly in adult insects or indirectly over bean seeds. These essential oils affected the development of A. obtectus since the greatest doses applied on beans decreased the emergence of the bean weevil. The results prove the insecticidal capacity of the tested essential oils and hence their potential as active substances against A. obtectus in environmentally low risk pest control strategies. Supplementary trials should be conducted under real storage conditions.

This study presents the main advances made in recent years by an innovative teaching group at the University of Almeria, who are attempting to incorporate alternative spaces for teaching–learning interaction. This group is developing a new line of study to obtain the main vectors for creating an accessible 3D virtual campus on the Second Life platform, recognized as a multi-user virtual environment (MUVE). The goal of this work was to measure the satisfaction and perception of a group of university students. To do this, a co-design approach based on student opinions by relating methodological procedures with an adaptation of the Kano model usually used for product design has been followed. The results found by a panel of postgraduate students in the second stage of the educational project, suggest that the synchronous distance teaching proposal developed can effectively facilitate conciliation of family, work and personal life with online teaching–learning. In the case of undergraduate students with class attendance, the use of the 3D virtual campus is perceived as something more complementary.

Mycobacterium bovis (Mb) is the causative agent of bovine tuberculosis (bTb). Genetic selection aiming to identify less susceptible animals has been proposed as a complementary measure in ongoing programs toward controlling Mb infection. However, individual animal phenotypes for bTb based on interferon-gamma (IFNɣ) and its use in bovine selective breeding programs have not been explored. In the current study, IFNɣ production was measured using a specific IFNɣ ELISA kit in bovine purified protein derivative (bPPD)-stimulated blood samples collected from Holstein cattle. DNA isolated from the peripheral blood samples collected from the animals included in the study was genotyped with the EuroG Medium Density bead Chip, and the genotypes were imputed to whole-genome sequences. A genome-wide association analysis (GWAS) revealed that the IFNɣ in response to bPPD was associated with a specific genetic profile (heritability = 0.23) and allowed the identification of 163 SNPs, 72 quantitative trait loci (QTLs), 197 candidate genes, and 8 microRNAs (miRNAs) associated with this phenotype. No negative correlations between this phenotype and other phenotypes and traits included in the Spanish breeding program were observed. Taken together, our results define a heritable and distinct immunogenetic profile associated with strong production of IFNɣ in response to Mb.

This study aimed to expand the knowledge about the activity and mode of action of CHI on methanogenesis and rumen microbial populations in vivo. A total of 16 lactating dairy cows were distributed in two groups, one of them receiving 135 mg CHI/kg body weight daily. The effect on productive performance, milk composition, fermentation efficiency, methane emissions, microbial protein synthesis, and ruminal microbial communities was determined. Supplementation with CHI did not affect rumen microbial diversity but increased the relative abundance (RA) of the bacteria Anaeroplasma and decreased those of rumen ciliates and protozoa resulting in a shift towards a lower acetic to propionic ratio. However, no effect on milk yield or methane intensity was observed. In conclusion, supplementing 135 mg CHI/kg body weight increased the RA of Anaeroplasma and decreased those of rumen ciliates and protozoa, both being related to fiber degradation in the rumen in different ways and resulted in a shift of ruminal fermentation towards more propionate proportions, without affecting CH4 emissions, milk yield, or milk composition. Further research with higher doses would be necessary to assess the potential use of this additive as a methane inhibitor.

The mechanisms underlying host resistance to Mycobacterium avium subsp. paratuberculosis (MAP) infection are largely unknown. In the current study, we hypothesize that cows with an ability to produce higher levels of interferon-gamma (IFNɣ) might control MAP infection more successfully. To test this hypothesis, IFNɣ production was measured using a specific IFNɣ ELISA kit in avian purified protein derivative (aPPD)-stimulated blood samples collected from 152 Holstein cattle. DNA isolated from peripheral blood samples of the animals included in the study was genotyped with the EuroG Medium-Density Bead Chip, and the genotypes were imputed to whole-genome sequencing. A genome-wide association analysis (GWAS) revealed that high levels of IFNɣ in response to the aPPD were associated with a specific genetic profile (heritability = 0.64) and allowed the identification of 71 SNPs, 40 quantitative trait loci (QTL), and 104 candidate genes. A functional analysis using the 104 candidate genes revealed a significant enrichment of genes involved in the innate immune response and, more specifically, in necroptosis. Taken together, our results define a heritable and distinct immunogenetic profile associated with the production of high IFNɣ levels and with the capacity of the host to lyse MAP-infected macrophages by necroptosis.

In the last two decades, many detailed full transcriptomic studies on complex biological samples have been published and included in large gene expression repositories. These studies primarily provide a bulk expression signal for each sample, including multiple cell-types mixed within the global signal. The cellular heterogeneity in these mixtures does not allow the activity of specific genes in specific cell types to be identified. Therefore, inferring relative cellular composition is a very powerful tool to achieve a more accurate molecular profiling of complex biological samples. In recent decades, computational techniques have been developed to solve this problem by applying deconvolution methods, designed to decompose cell mixtures into their cellular components and calculate the relative proportions of these elements. Some of them only calculate the cell proportions (supervised methods), while other deconvolution algorithms can also identify the gene signatures specific for each cell type (unsupervised methods). In these work, five deconvolution methods (CIBERSORT, FARDEEP, DECONICA, LINSEED and ABIS) were implemented and used to analyze blood and immune cells, and also cancer cells, in complex mixture samples (using three bulk expression datasets). Our study provides three analytical tools (corrplots, cell-signature plots and bar-mixture plots) that allow a thorough comparative analysis of the cell mixture data. The work indicates that CIBERSORT is a robust method optimized for the identification of immune cell-types, but not as efficient in the identification of cancer cells. We also found that LINSEED is a very powerful unsupervised method that provides precise and specific gene signatures for each of the main immune cell types tested: neutrophils and monocytes (of the myeloid lineage), B-cells, NK cells and T-cells (of the lymphoid lineage), and also for cancer cells.

The use of sourdough for bread production involves fermentation, which is dominated by lactic acid bacteria (LAB) and yeast. Sourdough can be inoculated with a starter culture or through a food matrix containing microorganisms to initiate sourdough fermentation. Sourdough is used as leavening agent for bread making, and metabolites produced by LAB and yeast confer a specific aroma and flavor profile to bread, thus improving its sensory attributes. However, few publications report the effect of microorganisms from different food products and by-products on sourdough fermentation. This review focuses on using different starter cultures from various food sources, from wheat flour to starter cultures. Additionally, included are the types of sourdough, the sourdough fermentation process, and the biochemical transformations that take place during the sourdough fermentation process.

During the fermentation of cocoa beans, the yeasts produce volatile organic compounds (VOCs). Through reactions associated with amino acid metabolism, yeasts generate important aroma precursors as acetate esters and fatty acid ethyl esters are essential in developing fruity flavors and aromas in the final product (usually chocolate). In addition, some yeasts may have pectinolytic and antifungal activity, which is desirable in the post-harvest process of cocoa. The main yeast species in cocoa fermentation are Saccharomyces cerevisiae, Pichia kudriavzevii, and Hanseniaspora opuntiae. These produce higher alcohols and acetyl-CoA to make acetate–esters, compounds that produce floral and fruity notes. However, there are still controversies in scientific reports because some mention that there are no significant differences in the sensory characteristics of the final product. Others mention that the fermentation of cocoa by yeast has a significant influence on improving the sensory attributes of the final product. However, using yeasts as starter cultures for cocoa bean fermentation is recommended to homogenize sensory attributes such as notes and flavors in chocolate.

We propose a detailed method for the interconnection between optical fibers and waveguides of photonic integrated circuits. Appropriate modal transmission is accomplished by matching the mode field diameters from both waveguide structures. Links from one structure to another are created by an interconnecting waveguide, maintaining a fixed coupling efficiency as its size is modified to adjust to the target waveguide core. This tailored transition acts as a mode field adapter, equalizing the transmission among multiple modes and reducing the mode-dependent losses while coupling. We present an algorithm to design the mode field adapter based on matching the effective mode areas using the power overlap integral. A study case considering a polymer photonic integrated device immediately connected to a few-mode fiber is analyzed. Coupling efficiencies over 90% for every transmitted mode are achieved, showing an evident improvement compared to typical approaches only matching core sizes. Detailed comparison of the results for each transmission mode is presented. This same procedure can be used to interconnect optical waveguides with different refractive index profiles and core geometry.

The demand of foods with high antioxidant capacity have increased and research on these foods continues to grow. This review is focused on chlorogenic acids (CGAs) from green coffee, which is the most abundant source. The main CGA in coffee is 5-O-caffeoylquinic acid (5-CQA). Coffee extracts are currently the most widely used source to enhance the antioxidant activity of foods. Due to the solubility of CGAs, their extraction is mainly performed with organic solvents. CGAs have been associated with health benefits, such as antioxidant, antiviral, antibacterial, anticancer, and anti-inflammatory activity, and others that reduce the risk of cardiovascular diseases, type 2 diabetes, and Alzheimer’s disease. However, the biological activities depend on the stability of CGAs, which are sensitive to pH, temperature, and light. The anti-inflammatory activity of 5-CQA is attributed to reducing the proinflammatory activity of cytokines. 5-CQA can negatively affect colon microbiota. An increase in anthocyanins and antioxidant activity was observed when CGAs extracts were added to different food matrices such as dairy products, coffee drinks, chocolate, and bakery products. The fortification of foods with coffee CGAs has the potential to improve the functionality of foods.

This study analyzes the spatio-temporal dynamics of the drivers of forest expansion in the Iberian Peninsula for the periods 1987–2002–2017 through a 185 km-wide north–south Landsat scene transect. The analysis has considered a variety of biogeographical regions [0–3500 m.a.s.l, annual rainfalls 150–2200 mm] and 30 explanatory variables. A rigorous map production at 30 m resolution, including detailed filtering methods and uncertainty management at pixel scale, provided high-quality land cover maps. The main forest expansion trajectories were related to explanatory variables using boosted regression trees. Proximity to previous forests was a key common factor for forest encroachment in all forest types, with other factors being distance to the hydrographic network, temperature and precipitation for broadleaf deciduous forests (BDF), precipitation, temperature and solar radiation for broadleaf evergreen forests (BEF) and precipitation, distance to province capitals, and solar radiation for needleleaf evergreen forests (NEFs). Results also showed contrasting forest expansion trajectories and drivers per biogeographic region, with a high dynamism of grasslands towards new forest in the Eurosiberian and the mountainous Mediterranean regions, a high importance of croplands as land cover origin of new forest in the Mesomediterranean, and increasing importance over time of socioeconomic drivers (such as those employed in the industry sector and the utilized agricultural area) in the Supramediterranean region but the opposite pattern in the Southern Mesomediterranean. Lower precipitation rates favored new NEFs from shrublands in the Thermomediterraean region which, together with the Northern Mesomediterranean, exhibited the highest relative rates of new forests. These findings provide reliable insights to develop policies considering the ecological and social impacts of land abandonment and subsequent forest expansion.

Current pharmacological therapies against demyelinating diseases are not quite satisfactory to promote remyelination. Epidermal growth factor (EGF) can expand the population of oligodendrocyte precursor cells (OPCs) that may help with the remyelination process, but its delivery into the injured tissue is still a biomedical challenge. Gold nanoparticles (GNPs) may be a useful tool for drug delivery into the brain. To evaluate remyelination in the septal nucleus, we administered intracerebral GNPs coupled with EGF (EGF–GNPs). C57BL6/J mice were demyelinated with 0.4% cuprizone (CPZ) and divided into several groups: Sham, Ctrl, GNPs, EGF, and EGF–GNPs. We evaluated the remyelination process at two time-points: 2 weeks and 3 weeks post-injection (WPI) of each treatment. We used the rotarod for evaluating motor coordination. Then, we did a Western blot analysis myelin-associated proteins: CNPase, MAG, MOG, and MBP. EGF–GNPs increase the expression of CNPase, MAG, and MOG at 2 WPI. At 3 WPI, we found that the EGF–GNPs treatment improves motor coordination and increases MAG, MOG, and MBP. EGF–GNPs enhance the expression of myelin-associated proteins and improve the motor coordination in mice. Thus, EGF-associated GNPs may be a promising pharmacological vehicle for delivering long-lasting drugs into the brain.

Farmland abandonment has been a widespread land-use change in the Iberian Peninsula since the second half of the 20th century, leading to the establishment of secondary forests across the region. In this study, we aimed to address changes in the recent (1985–2014) emergence patterns of these forests and examine how environmental factors affected their growth by considering differences in leaf-habit types. We used a combination of Landsat-derived land-cover maps and aboveground biomass (AGB) maps from the European Space Agency to assess the secondary forest establishment and growth, respectively, in the study region. We also obtained a set of topographic, climatic and landscape variables from diverse GIS layers and used them for determining changes over time in the environmental drivers of forest establishment and AGB using general linear models. The results highlight that secondary forest cover was still increasing in the Iberian Peninsula at a rate above the European average. Yet, they also indicate a directional change in the emergence of secondary forests towards lower and less steep regions with higher water availability (mean rainfall and SPEI) and less forest cover but are subjected to greater drought events. In addition, these environmental factors differentially affect the growth of forests with different leaf-habit types: i.e., needleleaf secondary forests being less favoured by high temperature and precipitation, and broadleaf deciduous forests being most negatively affected by drought. Finally, these spatial patterns of forest emergence and the contrasting responses of forest leaf-habits to environmental factors explained the major development of broadleaf evergreen compared to broadleaf deciduous forests and, especially, needleleaf secondary forests. These results will improve the knowledge of forest dynamics that have occurred in the Iberian Peninsula in recent decades and provide an essential tool for understanding the potential effects of climate warming on secondary forest growth.

The efficacy of intraperitoneal injection of an oil-based bivalent autogenous vaccine and the commercial vaccine AlphaJect 3000 (Pharmaq AS) to prevent atypical furunculosis and vibriosis in turbot was analyzed. The effect of both vaccines on health parameters and survival of fish after challenge with V. anguillarum and A. salmonicida subsp. achromogenes was tested. The autogenous vaccine conferred high levels of protection and long-lasting immunity against both pathogens with a single dose. However, severe side effects were observed in turbot injected with this autovaccine and minor negative effects with the AlphaJect 3000 vaccine and the adjuvant Montanide or Eolane. All vaccinated fish showed remarkable antibody agglutination titers, higher than those of control fish, which were maintained 160 d after vaccination. In conclusion, the autogenous bivalent vaccine induces long-lasting protection against atypical furunculosis and vibriosis in turbot, after administration of a single dose, at the cost of high side effects in fish. Therefore, the development of new vaccines should focus on autovaccines and the use of liquid paraffin adjuvants that increase protection with reduced or no side effects.

Network operators have been dealing with the necessity of a dynamic network resources allocation to provide a new generation of customer-tailored applications. In that sense, Telecom providers have to migrate their BSS/OSS systems and network infrastructure to more modern solutions to introduce end-to-end automation and support the new use cases derived from the 5G adoption and transport network slices. In general, there is a joint agreement on making this transition to an architecture defined by programmable interfaces and standard protocols. Hence, this paper uses the iFusion architecture to control and program the network infrastructure. The work presents an experimental validation of the network slicing instantiation in an IP/Optical environment using a set of standard protocols and interfaces. The work provides results of the creation, modification and deletion of the network slices. Furthermore, it demonstrates the usage of standard communication protocols (Netconf and Restconf) in combination with standard YANG data models.

Effects of substituting of wheat flour with coffee cherry pulp powder (CCPP) (coffee by-product as fiber source) at 0, 1.2, 2.3, and 4.7% dry basis (0, 1.25, 2.5, and 5% wet basis) on dough and gluten rheological properties and baking quality were investigated. Rheological properties were analyzed during mixing, compression recovery, and creep-recovery. A rheological approach was adopted to study the viscoelasticity of dough enriched with fiber. The data obtained were analyzed with the Kelvin–Voigt model and the parameters were correlated to bread volume and crumb firmness to assess the effect of incorporating CCPP. A decrease in gluten’s elastic properties was attributed to the water-binding and gelling properties of CCPP. Stiffness of dough and crumb firmness increased as the level of CCPP increased and bread volume decreased. Stiffer dough corresponded with lower compliance values and higher steady state viscosity compared to the control. A follow-up study with 5% CCPP and additives is recommended to overcome the reduction in elastic recovery and bread volume.

In the coastal zones, varied uses converge, some of them of priority interest. In this study, an integrated method for the planning and management of the territory is proposed, which includes the evaluation of sustainability. A total of 15 different land-use classes were estimated in 80 sampling units distributed regularly along the Pacific coastline of Nicaragua and classified to determine land management sectors. For each of the identified sectors, the ecological, economic, social, and productive dimensions were evaluated independently, handling a total of 53 variables from different databases, by means of ordination multivariate factor analysis. Subsequently, the four dimensions were integrated into a model and the results were evaluated based on their similarity with theoretical development scenarios, assessed by discriminant analysis. Among these, the scenarios considered as a goal for sustainability in the studied area were present. On the Pacific coast of Nicaragua, productive and economic activities are currently prioritized, without having an integrated planning scheme for the entire territory, which includes nature conservation. The main contribution has been to provide a method for evaluating the land in an integrative and multidimensional way, while at the same time qualifying the different territorial sectors from a sustainable development. Even under a context of relative scarcity of information for some relevant aspects, the dimension-values assessment is largely solved by ordering the territorial sectors with a multivariate strategy, so that they are classified in relative and not absolute terms, which allows the strategy to be very useful for countries lacking some databases and cartography. This holistic and comprehensive vision of the entire territory facilitates social participation and contributes to decision-making aimed at advancing toward sustainability.

Autism Spectrum Disorder (ASD) is an early neurodevelopmental disorder that involves deficits in interpersonal communication, social interaction, and repetitive behaviors. Although ASD pathophysiology is still uncertain, alterations in the abnormal development of the frontal lobe, limbic areas, and putamen generate an imbalance between inhibition and excitation of neuronal activity. Interestingly, recent findings suggest that a disruption in neuronal connectivity is associated with neural alterations in white matter production and myelination in diverse brain regions of patients with ASD. This review is aimed to summarize the most recent evidence that supports the notion that abnormalities in the oligodendrocyte generation and axonal myelination in specific brain regions are involved in the pathophysiology of ASD. Fundamental molecular mediators of these pathological processes are also examined. Determining the role of alterations in oligodendrogenesis and myelination is a fundamental step to understand the pathophysiology of ASD and identify possible therapeutic targets.

Gentiana lutea L. (G. lutea L.) is an endangered plant, patchily distributed along the mountains of Central and Southern Europe. In this study, inter-simple sequence repeat (ISSR) markers were used to investigate the genetic variation in this species within and among populations of G. lutea L. var. aurantiaca of the Cantabrian Mountains (Northwest Iberian Peninsula). Samples of G. lutea L. collected at different locations of the Pyrenees and samples of G. lutea L. subsp. vardjanii of the Dolomites Alps were also analyzed for comparison. Using nine ISSR primers, 106 bands were generated, and 89.6% of those were polymorphic. The populations from the Northwest Iberian Peninsula were clustered in three different groups, with a significant correlation between genetic and geographic distances. Gentiana lutea L. var. aurantiaca showed 19.8% private loci and demonstrated a remarkable level of genetic variation, both among populations and within populations; those populations with the highest level of isolation show the lowest genetic variation within populations. The low number of individuals, as well as the observed genetic structure of the analyzed populations makes it necessary to protect them to ensure their survival before they are too small to persist naturally.

The aim of this trial was to study the agreement between the non-dispersive infrared methane analyzer (NDIR) method and the hand held laser methane detector (LMD). Methane (CH4) was measured simultaneously with the two devices totaling 164 paired measurements. The repeatability of the CH4 concentration was greater with the NDIR (0.42) than for the LMD (0.23). However, for the number of peaks, repeatability of the LMD was greater (0.20 vs. 0.14, respectively). Correlation was moderately high and positive for CH4 concentration (0.73 and 0.74, respectively) and number of peaks (0.72 and 0.72, respectively), and the repeated measures correlation and the individual-level correlation were high (0.98 and 0.94, respectively). A moderate concordance correlation coefficient was observed for the CH4 concentration (0.62) and for the number of peaks (0.66). A moderate-high coefficient of individual agreement for the CH4 concentration (0.83) and the number of peaks (0.77) were observed. However, CH4 concentrations population means and all variance components differed between instruments. In conclusion, methane concentration measurements obtained by means of NDIR and LMD cannot be used interchangeably. The joint use of both methods could be considered for genetic selection purposes or for mitigation strategies only if sources of disagreement, which result in different between-subject and within-subject variabilities, are identified and corrected for.

The colonic epithelium is exposed to a mixture of compounds through diet, among which some are procarcinogens, whereas others have a protective effect. Therefore, the net impact of these compounds on human health depends on the overall balance between all factors involved. Strong scientific evidence has demonstrated the relationship between nitrosamines (NA), heterocyclic amines (HCAs), and polycyclic aromatic hydrocarbons (PAHs), which are the major genotoxins derived from cooking and food processing, and cancer. The mechanisms of the relationship between dietary toxic xenobiotics and cancer risk are not yet well understood, but it has been suggested that differences in dietary habits affect the colonic environment by increasing or decreasing the exposure to mutagens directly and indirectly through changes in the composition and activity of the gut microbiota. Several changes in the proportions of specific microbial groups have been proposed as risk factors for the development of neoplastic lesions and the enrichment of enterotoxigenic microbial strains in stool. In addition, changes in the gut microbiota composition and activity promoted by diet may modify the faecal genotoxicity/cytotoxicity, which can be associated with a higher or lower risk of developing cancer. Therefore, the interaction between dietary components and intestinal bacteria may be a modifiable factor for the development of colorectal cancer in humans and deserves more attention in the near future.

Boron and tin complexes have been a versatile and very interesting scaffold for the design of nonlinear optical (NLO) chromophores. In this paper we present a wide range of reports since the 1990s to date, which include second-order (e.g., second harmonic generation) and third-order (e.g., two-photon absorption) NLO properties. After a short introduction on the origin of the NLO response in molecules, the different features associated with the introduction of these inorganic motifs in the organic-based NLO materials are discussed: Their effect on the accepting/donating capabilities of the substituents, on the efficiency of the π-conjugated linkage, and on the topology of the chromophores which can be tuned from the first generation of “push-pull„ chromophores to more sophisticated two- or three-dimensional architectures.

The purpose of this study has been to conduct an energy diagnosis research of the third level. An energy diagnosis is a tool aimed to seek improvements related to measures of energy efficiency and saving, and environmental conservation as well. These improvements would prove to be relevant for any kind of building. The diagnosis was carried out in 36 university buildings (Renewable Energy Institute—REI) through survey and census; that in order (to identify and) to characterize current patterns of energy consumption and demand, as well as for generating specific strategies towards energy efficiency and saving, for instance the identification of systemic indicators and corrective proposals, and non-financial investment. The results promote the achievement of grand energy efficiency. The task on energy conservation entails the supporting participation of each person who either studies or works in the entity, whereas some other activities require economic stimulus for being implemented. Annual per capita energy consumption in the REI is 40 kWh/person: that is greater than the country’s average consumption rate. The main energy consumption corresponds to Building 3.1 (49% of total energy consumption). This is due to its large concentration of laboratories specialized in renewable energy research and innovation.

The use of Pseudoinvariant Areas (PIA) makes it possible to carry out a reasonably robust and automatic radiometric correction for long time series of remote sensing imagery, as shown in previous studies for large data sets of Landsat MSS, TM, and ETM+ imagery. In addition, they can be employed to obtain more coherence among remote sensing data from different sensors. The present work validates the use of PIA for the radiometric correction of pairs of images acquired almost simultaneously (Landsat-7 (ETM+) or Landsat-8 (OLI) and Sentinel-2A (MSI)). Four pairs of images from a region in SW Spain, corresponding to four different dates, together with field spectroradiometry measurements collected at the time of satellite overpass were used to evaluate a PIA-based radiometric correction. The results show a high coherence between sensors (r2 = 0.964) and excellent correlations to in-situ data for the MiraMon implementation (r2 > 0.9). Other methodological alternatives, ATCOR3 (ETM+, OLI, MSI), SAC-QGIS (ETM+, OLI, MSI), 6S-LEDAPS (ETM+), 6S-LaSRC (OLI), and Sen2Cor-SNAP (MSI), were also evaluated. Almost all of them, except for SAC-QGIS, provided similar results to the proposed PIA-based approach. Moreover, as the PIA-based approach can be applied to almost any image (even to images lacking of extra atmospheric information), it can also be used to solve the robust integration of data from new platforms, such as Landsat-8 or Sentinel-2, to enrich global data acquired since 1972 in the Landsat program. It thus contributes to the program’s continuity, a goal of great interest for the environmental, scientific, and technical community.

This manuscript explores numerical errors in highly anisotropic diffusion problems. First, the paper addresses the use of regular structured meshes in numerical solutions versus meshes aligned with the preferential directions of the problem. Numerical diffusion in structured meshes is quantified by solving the classical anisotropic diffusion problem; the analysis is exemplified with the application to a numerical model of conducting fluids under magnetic confinement, where rates of transport in directions parallel and perpendicular to a magnetic field are quite different. Numerical diffusion errors in this problem promote the use of magnetic field aligned meshes (MFAM). The generation of this type of meshes presents some challenges; several meshing strategies are implemented and analyzed in order to provide insight into achieving acceptable mesh regularity. Second, Gradient Reconstruction methods for magnetically aligned meshes are addressed and numerical errors are compared for the structured and magnetically aligned meshes. It is concluded that using the latter provides a more correct and straightforward approach to solving problems where anisotropicity is present, especially, if the anisotropicity level is high or difficult to quantify. The conclusions of the study may be extrapolated to the study of anisotropic flows different from conducting fluids.