1. Paper-Based Multiplexed Colorimetric Device for the Simultaneous Detection of Salivary Biomarkers
- Author
-
Tania Pomili, Pier Paolo Pompa, and Paolo Donati
- Subjects
Paper ,Materials science ,Clinical Biochemistry ,Metal Nanoparticles ,Spectral shift ,paper-based ,Multiplexing ,Article ,Humans ,Salivary biomarkers ,Point of care ,saliva ,Co2 laser ,multiplexing ,business.industry ,General Medicine ,Paper based ,point-of-care ,colorimetric test ,Colloidal gold ,Optoelectronics ,Colorimetry ,Gold ,business ,Biomarkers ,TP248.13-248.65 ,Biotechnology - Abstract
In this study, we describe a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The pattern of the proposed multiplexed device is designed with a central sample deposition zone and three identical arms, each containing a pre-treatment and test zone. Its one-step fabrication is realized by CO2 laser cutting, providing remarkable parallelization and rapidity (ca. 5 s/device). The colorimetric detection is based on the sensitive and selective target-induced reshaping of plasmonic multibranched gold nanoparticles, which exhibit a clear spectral shift (and blue-to-pink color change) in case of non-physiological concentrations of the three salivary biomarkers. A rapid and multiplexed naked-eye or smartphone-based readout of the colorimetric response is achieved within 10 min. A prototype kit for POCT testing is also reported, providing robustness and easy handling of the device.
- Published
- 2021