1. Molecular Characterization of Isolates of the Banana Bunchy Top Virus (BBTV) from the District of Chókwè, Mozambique.
- Author
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Barros, Sandra Carvalho I. Mussa, Figueira, Antonia dos Reis, and Silveira, Antonia Thalyta Lopes
- Subjects
BANANAS ,VIRAL transmission ,OCEAN - Abstract
Featured Application: The present work can contribute to understanding the origin of the Banana bunchy top virus isolates that occur in Mozambique, allowing phytosanitary organizations to trace the trajectory of the spread of the virus on the African continent and support control measures to avoid further damage. Banana bunchy top virus (BBTV) was recently detected in Mozambique and appears to be limited to the provinces of Gaza, Maputo and Zambezia, but it has great potential to spread to other provinces. Despite its importance, nothing is known about the BBTV isolates that occur in Mozambique. In this study, the sequences of the S and R genes of forty isolates chosen as representatives of samples collected previously from eleven farms of the four administrative posts of the district of Chóckwè, province of Gaza, were sequenced and analyzed. The S-DNA nucleotide sequences of the analyzed isolates were highly conserved, with identity ranging from 97% to 100%. The same was observed for the R-DNA sequences, with most identities ranging between 98% and 100% among the isolates from Chókwè and above 90% when compared to the isolates from GenBank. The phylogenetic analysis showed that the Mozambican BBTV isolates belong to the Pacific–Indian Oceans (PIO) group, showing greater proximity to the isolate JQ820453 from Malawi than to the isolates from sub-Saharan countries, which were grouped in a distinct subclade. This is the first study conducted to determine the molecular characteristics of BBTV isolates present in Mozambique. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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