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The attachment strength of bacteria to surfaces can affect the efficacy of sanitizers during washing. This study aimed to determine the effectiveness of chlorination and aeration in the removal of pathogens from the surface of produce. Cucumbers and bell peppers were inoculated with Listeria innocua, Escherichia coli O157:H7, or Salmonella enterica; afterwards, the produce was washed with or without chlorinated water (100 ppm) for 3 min in combination with or without aeration. Cucumbers washed with chlorinated water, with or without aeration, presented significant reductions of L. innocua (3.65 log CFU/cm2 and 1.13 log CFU/cm2, respectively) (p < 0.05). Similarly, bell peppers washed in chlorinated water with aeration (1.91 log CFU/g) and without aeration (2.49 log CFU/g) presented significant reductions of L. innocua. A significant reduction of L. innocua was observed on bell peppers washed with non-chlorinated water with aeration (2.49 log CFU/g) (p < 0.05). Non-chlorinated water was also effective in significantly reducing the level of Salmonella enterica (p < 0.05) on cucumbers and bell peppers. Washing with chlorinated water with aeration reduced Salmonella enterica levels from 4.45 log CFU/cm2 on cucumbers to below the detectable limit (0.16 log CFU/cm2). The highest reduction of Salmonella enterica from bell peppers occurred after washing with chlorinated water with aeration (2.48 log CFU/g). E. coli O157:H7, L. innocua, and Salmonella enterica levels present in non-chlorinated water after washing contaminated produce with or without aeration were significantly greater than those in chlorinated water (p < 0.05). After treatment, the population levels of all pathogens in chlorinated water with or without aeration were below the detectable limit for bell peppers (<1.10 log CFU/mL) and cucumbers (<1.20 log CFU/mL). Using chlorine in combination with forced aeration during washing minimizes cross-contamination of bacterial pathogens. [ABSTRACT FROM AUTHOR]

On-farm composting of agro-livestock wastes can be considered the most appropriate method for their recycling. Pile turning (PW) is one of the most widely used aeration systems for composting. However, this system has long composting periods and is inefficient at supplying oxygen and controlling the temperature. To minimize these drawbacks, the combination of turnings with forced aeration (PR) is an option; in this work, this combination was compared to PW as an aeration system for the co-composting of vegetable waste with different manures. In this comparative study, the evolution of the process, the compost quality and the economic and environmental impacts of the process were evaluated. The PR system was more appropriate for obtaining sanitized composts (the temperature was ≥55 °C for at least three consecutive days) with an adequate degree of maturity. Furthermore, this system reduced the organic matter and nutrient losses, yielding composts with higher agronomic value and a higher total combined value of the nutrients than those obtained using the PW system. However, the energy consumption and associated CO2 emissions were lower for the PW system, since this aeration system was based only on turnings without the use of forced aeration, as in the case of the PR system. Agricultural valorization of composts will offset this energy consumption and its impact, since it will contribute to reducing the use of synthetic fertilizers. However, more studies are required on the PR composting system and other agro-livestock wastes for the creation of centralized on-farm composting sites, where all steps of the composting chain are optimized. [ABSTRACT FROM AUTHOR]

Packed-bed bioreactors are often used for aerobic solid-state fermentation, since the forced aeration supplies O2 and removes metabolic heat from the bed. Motivated by the potential for applications in biorefineries, we review studies conducted on packed-bed bioreactors over the last decade, evaluating the insights these studies provide into how large-scale packed beds should be designed and operated. Many studies have used low superficial air velocities and suffer from preferential airflow, such that parts of the bed are not properly aerated. Moreover, some studies have proposed ineffective strategies, such as reversing the direction of the airflow or introducing air through perforated pipes within the bed. Additionally, many studies have used narrow water-jacketed packed-bed bioreactors, but these bioreactors do not reflect heat removal in wide large-scale packed beds, in which heat removal through the side walls makes a minor contribution. Finally, we conclude that, although some attention has been given to characterizing the porosities, water sorption isotherms and volumetric heat and mass transfer coefficients of substrate beds, this work needs to be extended to cover a wider range of solid substrates, and work needs to be done to characterize how these bed properties change due to microbial growth. [ABSTRACT FROM AUTHOR]