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Incorporating perennial pastures into annual crop systems is an efficient means of improving soil carbon (C) sequestration and reducing the application of nitrogen (N) fertilizer on farmlands. How the soil C and N at different soil depths respond to the length of pasture duration and rainfall conditions is still being determined. In this study, we conducted a meta-analysis of data from 63 published studies to investigate the impacts of the alfalfa pasture on the incorporation of soil organic carbon (SOC), total nitrogen (STN), and available nitrogen (SAN) contents in the 0–300 cm soil profile of the Loess Plateau. An annual crop field was taken as a reference. The results showed that the average SOC content at soil depths of 0–100 and 100–200 cm in the alfalfa pasture increased by 17% and 8% (p < 0.001) compared to the crop field, respectively, while that at 200–300 cm decreased (p > 0.05). The SOC content increased with pasture age; it was the highest when the alfalfa had been planted for 5–9 years and decreased thereafter. The STN content at soil depths of 0–100 and 100–200 cm increased by 19% and 14% (p < 0.001), respectively; the content at depths of 200–300 cm only increased slightly (p > 0.05). It also increased the most when the alfalfa was 5–9 years old. The increments in the SAN content at the 0–100 and 100–200 cm soil depths were higher than those of the STN, with values of 29% and 18%, respectively, while those at depths of 200–300 cm also changed insignificantly (p > 0.05). The SAN content continuously increased with the age of the alfalfa, and the average increment in the 0–300 cm profile was as high as 21% when the alfalfa was ≥10 years old. The SOC and STN content increased the most under moderate rainfall conditions (350–500 mm), while the SAN content maintained the highest increment under high rainfall (500–650 mm) conditions. Therefore, ley farming with the alfalfa pasture contributed substantially to the soil C and N at depths of 200 cm in deep loess. Alfalfa should be removed in its middle ages to increase C sequestration while utilizing soil N efficiently. [ABSTRACT FROM AUTHOR]