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This paper proposes a method that combines window functions with compressed sensing for the detection of ultra-high harmonics in the frequency range of 2–150 kHz. By analyzing the sparsity of the signal, the Bruckmann window function, which is most suitable for the compressed sensing reconstruction process and the characteristics of ultra-high harmonics, is selected. Simulations indicate that, compared to existing methods, the proposed algorithm based on the fusion of the Bruckmann window and compressed sensing achieves a sparser post-observation signal with reduced fluctuations. The robustness and anti-interference capabilities are enhanced, while the harmonic detection accuracy and signal reconstruction performance are significantly improved. The reconstruction error reaches 4.15 × 10−6, the mean squared error percentage (MSE) reaches 4.13 × 10−6, and the signal-to-noise ratio (SNR) is as high as 97.69 dB, marking an increase of 54.11%. This study provides a new theoretical and methodological approach for the analysis and processing of ultra-high harmonics caused by a high proportion of power electronic devices. [ABSTRACT FROM AUTHOR]

Fastener screws are critical components of rail fasteners. For the fastener screw maintenance robot, an image-based fast fastener screw detection method is urgently needed. In this paper, we propose a light-weight model named FSS-YOLO based on YOLOv5n for rail fastener screw detection. The C3Fast module is presented to replace the C3 module in the backbone and neck to reduce Params and FLOPs. Then, the SIoU loss is introduced to enhance the convergence speed and recognition accuracy. Finally, for the enhancement of the screw detail feature fusion, the shuffle attention (SA) is incorporated into the bottom-up process in the neck part. Experiment results concerning CIoU and DIoU for loss, MobileNetv3 and GhostNet for light-weight improvement, simple attention mechanism (SimAM), and squeeze-and-excitation (SE) attention for the attention module, and YOLO series methods for performance comparison are listed, demonstrating that the proposed FSS-YOLO significantly improves the performance, with higher accuracy and lower computation cost. It is demonstrated that the FSS-YOLO is 7.3% faster than the baseline model in FPS, 17.4% and 19.5% lower in Params and FLOPs, respectively, and the P, mAP@50, Recall, and F1 scores are increased by 10.6% and 6.4, 13.4%, and 12.2%, respectively. [ABSTRACT FROM AUTHOR]

With the development of high-power fuel cell vehicles, heat dissipation requirements have become increasingly stringent. Although conventional cooling techniques improve the heat dissipation capacity by increasing the fan rotating speed or radiator dimensions, high energy consumption and limited engine compartment space prevent their implementation. Moreover, the insufficient heat transfer capacity of existing coolants limits the enhancement of heat dissipation performance. Therefore, exploring novel coolants to replace traditional coolants is important. Nanofluids composed of nanoparticles and base liquids are promising alternatives, effectively improving the heat transfer capacity of the base liquid. However, challenges remain that prevent their use in fuel cell vehicles. These include issues regarding the nanofluid stability and cleaning, erosion and abrasion, thermal conductivity, and electrical conductivity. In this review, we summarize the nanofluid applications in oil-fueled, electric, and fuel cell vehicles. Subsequently, we provide a comprehensive literature review of the challenges and future research directions of nanofluids as coolants in fuel cell vehicles. This review demonstrates the potential of nanofluids as an alternative thermal management system that can facilitate transition toward a low-carbon, energy-secure economy. It will serve as a reference for researchers to focus on new areas that could drive the field forward. [ABSTRACT FROM AUTHOR]

Hydrogen is the most promising alternative fuel in the field of engines. Exhaust heat-assisted methanol dissociation is an attractive approach for generating hydrogen. In this work, simulations are conducted on a compression ignition engine fueled with different proportions of diesel-dissociated methanol gas (DMG) blends at intermediate engine speed, full load, and 0% EGR ratio. The results reveal that the indicated thermal efficiency and indicated mean effective pressure are greatly enhanced, combustion efficiency is increased, and regular emissions of CO, HC, and soot are reduced, while NOx emissions are reduced with increased DMG substitution. In addition, a simulation is conducted at an intermediate engine speed, full load, 15% DMG substitution ratio, and varying EGR ratios of 0–20%. The results indicate that the dual-fuel engine outperforms the original engine with respect to power, fuel economy, and regular emissions, once an optimal EGR rate is adopted. [ABSTRACT FROM AUTHOR]

The purpose of this work is to provide support for the design of cushioning packaging materials for products with unbalanced mass distributions. A mathematical model of the cushioning packaging system is established and the fourth-order Runge–Kutta method is used to compare the vibration response of products with balanced and unbalanced mass. Finite element simulations are conducted to demonstrate the feasibility of the proposed method of optimizing the design of cushioning packaging materials for products with unbalanced mass distributions using various damping materials. The simulation results are verified by experiments. The results show that the magnitude of the mass offset has a large effect on the acceleration of damped cushioning packaging systems for products with unbalanced mass. The vibration response can be corrected by appropriately increasing the damping coefficient at the mass offset end, thus providing an effective method of optimizing the design of cushioning packaging for products with unbalanced mass. [ABSTRACT FROM AUTHOR]

It is essentially important to develop durable polymer foams for services in high-temperature conditions. The current study reported the preparations and properties of a high-performance benzoxazine-phthalonitrile (BZPN) foam by utilizing azodicarbonamide and tween-80 as the blowing agent and stabilizer, respectively. Rheological and curing studies indicated that the appropriate foaming temperature for BZPN foam is below 180 °C, and its foaming viscosity window is below 20 Pa·s. Guided by these results, uniform millet bread-like BZPN foams with decimeter leveling size were successfully realized, suggesting the high prospect of large-scale production. The structural, mechanical, and thermal properties of BZPN foams were then investigated in detail. BZPN foam involves a hierarchical fracture mechanism during the compressive test, and it shows a high compression strength of over 6 MPa. During a burning test over 380 °C, no visible smoke, softening, or droplet phenomena appeared and the macroscopic structure of BZPN foam was well maintained. Mechanically robust, flame-retardant, and uniform large-size BZPN foam are promising light durable materials with high service temperatures, i.e., as filling materials even in a very narrow pipette. [ABSTRACT FROM AUTHOR]

Nobiletin (NOB) has attracted much attention owing to its outstanding bioactivities. This study aimed to investigate its anti-arrhythmic effect through electrophysiological and molecular docking studies. We assessed the anti-arrhythmic effects of NOB using aconitine-induced ventricular arrhythmia in a rat model and the electrophysiological effects of NOB on rat cardiomyocytes utilizing whole-cell patch-clamp techniques. Moreover, we investigated the binding characters of NOB with rNav1.5, rNav1.5/QQQ, and hNaV1.5 via docking analysis, comparing them with amiodarone and aconitine. NOB pretreatment delayed susceptibility to ventricular premature and ventricular tachycardia and decreased the incidence of fatal ventricular fibrillation. Whole-cell patch-clamp assays demonstrated that the peak current density of the voltage-gated Na+ channel current was reversibly reduced by NOB in a concentration-dependent manner. The steady-state activation and recovery curves were shifted in the positive direction along the voltage axis, and the steady-state inactivation curve was shifted in the negative direction along the voltage axis, as shown by gating kinetics. The molecular docking study showed NOB formed a π-π stacking interaction with rNav1.5 and rNav1.5/QQQ upon Phe-1762, which is the homolog to Phe-1760 in hNaV1.5 and plays an important role in antiarrhythmic action This study reveals that NOB may act as a class I sodium channel anti-arrhythmia agent. [ABSTRACT FROM AUTHOR]

Fire-detection technology is of great importance for successful fire-prevention measures. Image-based fire detection is one effective method. At present, object-detection algorithms are deficient in performing detection speed and accuracy tasks when they are applied in complex fire scenarios. In this study, a lightweight fire-detection algorithm, Light-YOLOv5 (You Only Look Once version five), is presented. First, a separable vision transformer (SepViT) block is used to replace several Cross Stage Partial Bottleneck with 3 convolutions (C3) modules in the final layer of a backbone network to enhance both the contact of the backbone network to global information and the extraction of flame and smoke features; second, a light bidirectional feature pyramid network (Light-BiFPN) is designed to lighten the model while improving the feature extraction and balancing speed and accuracy features during a fire-detection procedure; third, a global attention mechanism (GAM) is fused into the network to cause the model to focus more on the global dimensional features and further improve the detection accuracy of the model; and finally, the Mish activation function and SIoU loss are utilized to simultaneously increase the convergence speed and enhance the accuracy. The experimental results show that compared to the original algorithm, the mean average accuracy (mAP) of Light-YOLOv5 increases by 3.3%, the number of parameters decreases by 27.1%, and the floating point operations (FLOPs) decrease by 19.1%. The detection speed reaches 91.1 FPS, which can detect targets in complex fire scenarios in real time. [ABSTRACT FROM AUTHOR]

Fatty acid desaturases (FADs) modulate carbon–carbon single bonds to form carbon–carbon double bonds in acyl chains, leading to unsaturated fatty acids (UFAs) that have vital roles in plant growth and development and their response to environmental stresses. In this study, a total of 23 Populus trichocarpaFAD (PtFAD) candidates were identified from the poplar genome and clustered into seven clades, including FAB2, FAD2, FAD3/7/8, FAD5, FAD6, DSD, and SLD. The exon–intron compositions and conserved motifs of the PtFADs, clustered into the same clade, were considerably conserved. It was found that segmental duplication events are predominantly attributable to the PtFAD gene family expansion. Several hormone- and stress-responsive elements in the PtFAD promoters implied that the expression of the PtFAD members was complicatedly regulated. A gene expression pattern analysis revealed that some PtFAD mRNA levels were significantly induced by abiotic stress. An interaction proteins and gene ontology (GO) analysis indicated that the PtFADs are closely associated with the UFAs biosynthesis. In addition, the UFA contents in poplars were significantly changed under drought and salt stresses, especially the ratio of linoleic and linolenic acids. The integration of the PtFAD expression patterns and UFA contents showed that the abiotic stress-induced PtFAD3/7/8 members mediating the conversion of linoleic and linolenic acids play vital roles in response to osmotic stress. This study highlights the profiles and functions of the PtFADs and identifies some valuable genes for forest improvements. [ABSTRACT FROM AUTHOR]

The use of simple, inexpensive, and efficient methods to construct carbon–boron and carbon–oxygen bonds has been a hot research topic in organic synthesis. We demonstrated that the desired β-boronic acid products can be obtained under mild conditions using copper foam as an efficient heterogeneous catalyst. The structure of copper foam before and after the reaction was investigated by polarized light microscopy (PM), scanning electron microscopy (SEM), and transmission electron microscopy (TEM), and the results have shown that the structure of the catalyst copper foam remained unchanged before and after the reaction. The XPS test results showed that the Cu(0) content increased after the reaction, indicating that copper may be involved in the boron addition reaction. The specific optimization conditions were as follows: CH3COCH3 and H2O were used as mixed solvents, 4-methoxychalcone was used as the raw material, 8 mg of catalyst was used and the reaction was carried out at room temperature and under air for 10 h. The yield of the product obtained was up to 92%, and the catalytic efficiency of the catalytic material remained largely unchanged after five cycles of use. [ABSTRACT FROM AUTHOR]

Long-chain acyl-CoA synthetases (LACSs) catalyze fatty acids (FAs) to form fatty acyl-CoA thioesters, which play essential roles in FA and lipid metabolisms and cuticle wax biosynthesis. Although LACSs from Arabidopsis have been intensively studied, the characterization and function of LACSs from poplar are unexplored. Here, 10 poplar PtLACS genes were identified from the poplar genome and distributed to eight chromosomes. A phylogenetic tree indicated that PtLACSs are sorted into six clades. Collinearity analysis and duplication events demonstrated that PtLACSs expand through segmental replication events and experience purifying selective pressure during the evolutionary process. Expression patterns revealed that PtLACSs have divergent expression changes in response to abiotic stress. Interaction proteins and GO analysis could enhance the understanding of putative interactions among protein and gene regulatory networks related to FA and lipid metabolisms. Cluster networks and long-chain FA (LCFA) and very long-chain FA (VLCFA) content analysis revealed the possible regulatory mechanism in response to drought and salt stresses in poplar. The present study provides valuable information for the functional identification of PtLACSs in response to abiotic stress metabolism in poplar. [ABSTRACT FROM AUTHOR]

The utility of a solvent is one of the key factors that impacts resultant film morphology. However, the effect of solvent-dependent morphology on the doping process and electrical conductivity has not been adequately elucidated. In this work, we compared the morphology of chloroform- and chlorobenzene-processed thiophene polymer films and investigated how the choice of solvent influences film morphology, doping level, charge transport properties, and thus electrical conductivity. It was found that the film drop-casted from chloroform exhibits better crystallinity than that drop-casted from chlorobenzene. The crystallinity has negligible impact on the doping level but significant impact on charge transport properties. As a result, the chloroform-processed film shows a higher electrical conductivity of up to 408 S cm−1 due to a high carrier mobility related to the continuously crystalline domains in film. This finding indicates that the choice of solvent for preparation of film, which strongly correlated with molecular orientation, is a new strategy to optimize the electrical conductivity of doped polymers. [ABSTRACT FROM AUTHOR]

The recent advances in wearable sensors and intelligent human–machine interfaces have sparked a great many interests in conductive fibers owing to their high conductivity, light weight, good flexibility, and durability. As one of the most impressive materials for wearable sensors, conductive fibers can be made from a variety of raw sources via diverse preparation strategies. Herein, to offer a comprehensive understanding of conductive fibers, we present an overview of the recent progress in the materials, the preparation strategies, and the wearable sensor applications related. Firstly, the three types of conductive fibers, including metal-based, carbon-based, and polymer-based, are summarized in terms of their principal material composition. Then, various preparation strategies of conductive fibers are established. Next, the primary wearable sensors made of conductive fibers are illustrated in detail. Finally, a robust outlook on conductive fibers and their wearable sensor applications are addressed. [ABSTRACT FROM AUTHOR]

Featured Application: The research work highlights the great potential of application of loess-PHW mixtures to loess erosion control and slope stabilization. Loess and PHW (post-harvest waste) are easily accessible in the Chinese Loess Plateau and have been widely applied to construction of residential houses that have been inhabited for decades under the effect of freeze-thaw cycles. Although many researchers have recognised that the addition of fibers to loess soil is effective in preventing soil erosion and stabilising slopes, a consensus on this claim has not been reached yet. This study investigates the shearing behaviour of the loess-PHW mixture using small-scale and large-scale direct shear (SSDS and LSDS) tests. Four typical shear stress versus horizontal displacement curves from the multiscale direct shear tests are recognised where one is featured with strain-softening shape and the other three with a strain-hardening shape. Two out of the three curves with strain-hardening shape show a gradual increase in the shear stress at additional and larger displacements, respectively, in which some factor starts to have an influence on the shearing behaviour. Comparisons of the shear strength measured in SSDS and LSDS are made, indicating that there are differences between SSDS and LSDS. The effect of PHW addition on shear strength is assessed in order to determine the optimal dosage. The improvement of shear strength is attributed to the effect of particle inter-locking, resulting from the addition of PHW to loess specimens, and takes effect as the water content surpassed a threshold, i.e., >14%, that facilitates particle rearrangement. Particle-box interaction behaviour is assessed at the same time, and the findings satisfactorily address the main cause of the gradual increase in shear stress following the curve inflection point. The improved shearing behaviour proves the ability of the loess-PHW mixture to resist the seepage force and consequently stratum erosion. [ABSTRACT FROM AUTHOR]

As an important resource for screening microbial strains capable of conferring stress tolerance in plants, the fungal community associated with the plants grown in stressful environments has received great attention. In this study, high-throughput sequencing was employed to study the rhizosphere fungal community in the reclaimed area (i.e., sites F, H, and T) of the eastern coast of China. Moreover, endophytic fungi from the root of six plant species colonizing the investigated sites were isolated and identified. The differences in soil physicochemical parameters, fungal diversity, and community structure were detected among the sampling sites and between the seasons. Ectomycorrhizal (ECM) fungi (e.g., genera Tuber and Geopora) were dominant at site F, which was characterized by high soil total carbon (SC) and total nitrogen (SN) contents and low soil electrical conductivity (EC) value. Arbuscular mycorrhizal (AM) fungi, including genera Glomus, Rhizophagus, and Entrophospora were dominant at sites H (winter), H (summer), and T (summer), respectively. The positive relationship between the EC value and the abundance of genus Glomus indicated the ability of this AM fungus to protect plants against the salt stress. Endophytic fungi at sites F (Aspergillus and Tetracladium), H (Nigrospora), and T (Nigrospora, Coniochaeta and Zopfiella) were recognized as the biomarkers or keystone taxa, among which only genus Aspergillus was isolated from the plant roots. The aforementioned AM fungi and endophytic fungi could contribute to the promotion of plant growth in the newly reclaimed land. [ABSTRACT FROM AUTHOR]

Carotenoid cleavage dioxygenases (CCDs) catalyzes the cleavage of various carotenoids into smaller apocarotenoids which are essential for plant growth and development and response to abiotic stresses. CCD family is divided into two subfamilies: 9-cis epoxycarotenoid dioxygenases (NCED) family and CCD family. A better knowledge of carotenoid biosynthesis and degradation could be useful for regulating carotenoid contents. Here, 23 CCD genes were identified from the Populus trichocarpa genome, and their characterizations and expression profiling were validated. The PtCCD members were divided into PtCCD and PtNCED subfamilies. The PtCCD family contained the PtCCD1, 4, 7, and 8 classes. The PtCCDs clustered in the same clade shared similar intron/exon structures and motif compositions and distributions. In addition, the tandem and segmental duplications resulted in the PtCCD gene expansion based on the collinearity analysis. An additional integrated collinearity analysis among poplar, Arabidopsis, rice, and willow revealed the gene pairs between poplar and willow more than that between poplar and rice. Identifying tissue-special expression patterns indicated that PtCCD genes display different expression patterns in leaves, stems, and roots. Abscisic acid (ABA) treatment and abiotic stress suggested that many PtCCD genes are responsive to osmotic stress regarding the comprehensive regulation networks. The genome-wide identification of PtCCD genes may provide the foundation for further exploring the putative regulation mechanism on osmotic stress and benefit poplar molecular breeding. [ABSTRACT FROM AUTHOR]

Poplar is an illustrious industrial woody plant with rapid growth, providing a range of materials, and having simple post-treatment. Various kinds of environmental stresses limit its output. Plant annexin (ANN) is a calcium-dependent phospholipid-binding protein involved in plant metabolism, growth and development, and cooperatively regulating drought resistance, salt tolerance, and various stress responses. However, the features of the PtANN gene family and different stress responses remain unknown in poplar. This study identified 12 PtANN genes in the P. trichocarpa whole-genome and PtANNs divided into three subfamilies based on the phylogenetic tree. The PtANNs clustered into the same clade shared similar gene structures and conserved motifs. The 12 PtANN genes were located in ten chromosomes, and segmental duplication events were illustrated as the main duplication method. Additionally, the PtANN4 homogenous with AtANN1 was detected localized in the cytoplasm and plasma membrane. In addition, expression levels of PtANNs were induced by multiple abiotic stresses, which indicated that PtANNs could widely participate in response to abiotic stress. These results revealed the molecular evolution of PtANNs and their profiles in response to abiotic stress. [ABSTRACT FROM AUTHOR]

The study of the gravity response of roots and shoots is of great significance when exploring the polarity of plants and the development of the forest industry. In our study, normal and inverted cuts of Salix matsudana (Koidz) were cultured. The total RNAs of roots and shoots were extracted. Based on the comparative transcriptome, 412 and 668 genes were differentially expressed. The plasma membrane-, cell wall-, and extracellular region-related genes were up-regulated in the shoots, while the carbon metabolism and the nitrogen metabolism were up-regulated in the roots. Combining the alternative splicing genes, we found a potential gravity response network: in the shoots, LecRLKs were highly sensitive to gravity and further affected the alternative splicing of SNARE, as well as inducing an inhomogeneous distribution of auxin and a negative geotropism curve. In the roots, AP2/ERFs and STRKs were highly sensitive to gravity and regulated the expression level of STPKs and WAKs, finally resulting in a geotropism curve. Moreover, cell division was suppressed in both the roots and the shoots under inverted conditions with different mechanisms. Cell division inhibitors (KRPs) were up-regulated in the roots, while DNA helicase MCMs were down-regulated in the shoots. These results provide an important foundation for further studies of the molecular mechanisms and genetic regulation of plant responses to gravity and the plant polarity of forest trees. [ABSTRACT FROM AUTHOR]

MoS2 has been regarded as a promising addition for the preparation of epoxy-based coatings with high anticorrosion ability. However, its dispersion and compatibility remain significant challenges. In the present work, an organic thin layer was well coated on lamellar molybdenum disulfide (MoS2) via a simple modification of 3-aminopropyltriethoxysilane (APTES). The modification of hydrolyzing APTES on lamellar MoS2 effectively improved the dispersity of MoS2 in water-borne epoxy (WEP) and successfully enhanced the compatibility and crosslinking density of MoS2 with WEP. The influence of introducing MoS2-APTES into WEP coating on anticorrosion property for N80 steel was tested by electrochemical impedance spectroscopy (EIS), potentiodynamic polarization and salt spray test. The results exhibited that the |Z|0.01Hz value of MoS2-APTES/WEP still reached 3.647 × 107 Ω·cm2 even after the immersion time of 50 days in 3.5 wt.% NaCl solution, showing an extraordinary performance of corrosion resistance. The enhanced anticorrosion performance of composite coating could be resulted from the apparently increased dispersibility and compatibility of MoS2 in WEP. [ABSTRACT FROM AUTHOR]

Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter's protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN-γ production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine's efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD. [ABSTRACT FROM AUTHOR]

Porcine parvovirus (PPV) is an important pathogen causing reproductive failure in pigs. PPV-induced cell apoptosis has been recently identified as being involved in PPV-induced placental tissue damages resulting in reproductive failure. However, the molecular mechanism was not fully elucidated. Here we demonstrate that PPV nonstructural protein 1 (NS1) can induce host cell apoptosis and death, thereby indicating the NS1 may play a crucial role in PPV-induced placental tissue damages and reproductive failure. We have found that NS1-induced apoptosis was significantly inhibited by caspase 9 inhibitor, but not caspase 8 inhibitor, and transfection of NS1 gene into PK-15 cells significantly inhibited mitochondria-associated antiapoptotic molecules Bcl-2 and Mcl-1 expressions and enhanced proapoptotic molecules Bax, P21, and P53 expressions, suggesting that NS1-induced apoptosis is mainly through the mitochondria-mediated intrinsic apoptosis pathway. We also found that both PPV infection and NS1 vector transfection could cause host DNA damage resulting in cell cycle arrest at the G1 and G2 phases, trigger mitochondrial ROS accumulation resulting in mitochondria damage, and therefore, induce the host cell apoptosis. This study provides a molecular basis for elucidating PPV-induced cell apoptosis and reproductive failure. [ABSTRACT FROM AUTHOR]

Calcium phosphate nanoparticles (CPNPs) encapsulating small organic molecules, such as imaging agents and drugs, are considered to be ideal devices for cancer diagnosis or therapy. However, it is generally difficult to encapsulate small organic molecules in CPNPs because of the lack of solubility in water or binding affinity to calcium phosphate. To solve these issues, we utilized the carboxymethyl β-cyclodextrin (CM-β-CD) to increase the solubility and binding affinity to small organic molecules for the encapsulation into CPNPs in this work. The results indicated that the model molecules, hydrophilic rhodamine B (RB) and hydrophobic docetaxel (Dtxl), are successfully encapsulated into CPNPs with the assistance of CM-β-CD. We also demonstrated the CPNPs could be remarkably internalized into A549 cells, resulting in the efficient inhibition of tumor cells' growth., Competing Interests: The authors declare no competing financial interest.