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As one of the most important economic traits for domestic animal husbandry, skeletal muscle is regulated by an intricate molecular network. Adenosine deaminase acting on RNA (ADAR1) involves various physiological processes and diseases, such as innate immunity and the development of lung adenocarcinoma, breast cancer, gastric cancer, etc. However, its role in skeletal muscle growth requires further clarification. Here, we explored the functions of ADAR1 in the myogenic process of goat skeletal muscle satellite cells (MuSCs). The ADAR1 transcripts were noticeably enriched in goat visceral tissues compared to skeletal muscle. Additionally, its levels in slow oxidative muscles like the psoas major and minor muscles were higher than in the fast oxidative glycolytic and fast glycolytic muscles. Among the two common isoforms from ADAR1, p110 is more abundant than p150. Moreover, overexpressing ADAR1 enhanced the proliferation and myogenic differentiation of MuSCs. The mRNA-seq performed on MuSCs' knockdown of ADAR1 obtained 146 differentially expressed genes (DEGs), 87 upregulated and 59 downregulated. These DEGs were concentrated in muscle development and process pathways, such as the MAPK and cAMP signaling pathways. Furthermore, many DEGs as the key nodes defined by protein–protein interaction networks (PPI), including STAT3, MYH3/8, TGFβ2, and ACTN4, were closely related to the myogenic process. Finally, RNA immunoprecipitation combined with qPCR (RIP-qPCR) showed that ADAR1 binds to PAX7 and MyoD mRNA. This study indicates that ADAR1 promotes the myogenic development of goat MuSCs, which provides a useful scientific reference for further exploring the ADAR1-related regulatory networks underlying mammal skeletal muscle growth. [ABSTRACT FROM AUTHOR]

Skeletal muscle development is spotlighted in mammals since it closely relates to animal health and economic benefits to the breeding industry. Researchers have successfully unveiled many regulatory factors and mechanisms involving myogenesis. However, the effect of N6-methyladenosine (m6A) modification, especially demethylase and its regulated genes, on muscle development remains to be further explored. Here, we found that the typical demethylase FTO (fat mass- and obesity-associated protein) was highly enriched in goats' longissimus dorsi (LD) muscles. In addition, the level of m6A modification on transcripts was negatively regulated by FTO during the proliferation of goat skeletal muscle satellite cells (MuSCs). Moreover, a deficiency of FTO in MuSCs significantly retarded their proliferation and promoted the expression of dystrophin-associated protein 1 (DAG1). m6A modifications of DAG1 mRNA were efficiently altered by FTO. Intriguingly, the results of DAG1 levels and its m6A enrichment from FB23-2 (FTO demethylase inhibitor)-treated cells were consistent with those of the FTO knockdown, indicating that the regulation of FTO on DAG1 depended on m6A modification. Further experiments showed that interfering FTO improved m6A modification at site DAG1-122, recognized by Insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) and consequently stabilized DAG1 transcripts. Our study suggests that FTO promotes the proliferation of MuSCs by regulating the expression of DAG1 through m6A modification. This will extend our knowledge of the m6A-related mechanism of skeletal muscle development in animals. [ABSTRACT FROM AUTHOR]

In order to further enhance the erosion resistance of cement concrete pavement materials, this study constructed an apparent rough hydrophobic structure layer by spraying a micro-nano substrate coating on the surface layer of the cement concrete pavement. This was followed by a secondary spray of a hydroxy-silicone oil-modified epoxy resin and a low surface energy-modified substance paste, which combine to form a superhydrophobic coating. The hydrophobic mechanism of the coating was then analysed. Firstly, the effects of different types and ratios of micro-nano substrates on the apparent morphology and hydrophobic performance of the rough structure layer were explored through contact angle testing and scanning electron microscopy (SEM). Subsequently, Fourier transform infrared spectroscopy and permeation gel chromatography were employed to ascertain the optimal modification ratio, temperature, and reaction mechanism of hydroxy-silicone oil with E51 type epoxy resin. Additionally, the mechanical properties of the modified epoxy resin-low surface energy-modified substance paste were evaluated through tensile tests. Finally, the erosion resistance of the superhydrophobic coating was tested under a range of conditions, including acidic, alkaline, de-icer, UV ageing, freeze-thaw cycles and wet wheel wear. The results demonstrate that relying solely on the rough structure of the concrete surface makes it challenging to achieve superhydrophobic performance. A rough structure layer constructed with diamond micropowder and hydrophobic nano-silica is less prone to cracking and can form more "air chamber" structures on the surface, with better wear resistance and hydrophobic performance. The ring-opening reaction products that occur during the preparation of modified epoxy resin will severely affect its mechanical strength after curing. Controlling the reaction temperature and reactant ratio can effectively push the modification reaction of epoxy resin through dehydration condensation, which produces more grafted polymer. It is noteworthy that the grafted polymer content is positively correlated with the hydrophobicity of the modified epoxy resin. The superhydrophobic coating exhibited enhanced erosion resistance (based on hydrochloric acid), UV ageing resistance, abrasion resistance, and freeze-thaw damage resistance to de-icers by 19.41%, 18.36%, 43.17% and 87.47%, respectively, in comparison to the conventional silane-based surface treatment. [ABSTRACT FROM AUTHOR]

The Tian Shan is a typical active intracontinental orogenic belt that is driven by the ongoing indentation of India into Eurasia. However, the geological features of Quaternary deformation, especially in the easternmost sector near Harlik Mountain, remain elusive. Field observations, topographic analysis, and Electron Spin Resonance (ESR) dating were employed to comprehensively assess the deformation features and evaluate the deformation pattern for this region during the Quaternary period. The results disclose evidence of deformation in the northern and southern foreland basins of Harlik Mountain. In the Barkol Basin to the north, crustal shortening results in the formation of surface scarps and folds, indicating north-directed thrusting, with a shortening rate of ~0.15 mm/yr. In the Hami Basin, the north-directed thrust elevates the granites, which offset the alluvial fans, with a shortening rate of ~0.18 mm/yr. Together with the shortening along the boundary fault, the aggregated north–south shortening rate is approximately 0.69 mm/yr in the easternmost Tian Shan, corresponding with the differential motion rate between the north and south Harlik Mountain revealed by the GPS velocity. These findings imply that, distal to the collision zone, tectonic strain in the eastern Tian Shan is primarily accommodated through the reactivation of pre-existing strike–slip faults, with crustal shortening concentrated at the overlapping position of parallel northeast-trending left-lateral strike–slip faults. [ABSTRACT FROM AUTHOR]

The anterior gradient protein 2 (AGR2) plays a crucial role in facilitating the formation of protein disulfide bonds within the endoplasmic reticulum (ER). Research suggests that AGR2 can function as an oncogene, with its heightened expression linked to the advancement of hepatobiliary and pancreatic cancers through invasion and metastasis. Notably, AGR2 not only serves as a pro-oncogenic agent but also as a downstream targeting protein, indirectly fostering cancer progression. This comprehensive review delves into the established functions and expression patterns of AGR2, emphasizing its pivotal role in cancer progression, particularly in hepatobiliary and pancreatic malignancies. Furthermore, AGR2 emerges as a potential cancer prognostic marker and a promising target for immunotherapy, offering novel avenues for the treatment of hepatobiliary and pancreatic cancers and enhancing patient outcomes. [ABSTRACT FROM AUTHOR]

SO2 plays an important role in wine fermentation, and its effects on wine aroma are complex and diverse. In order to investigate the effects of different SO2 additions on the fermentation process, quality, and flavor of 'Beibinghong' ice wine, we fermented 'Beibinghong' picked in 2019. We examined the fermentation rate, basic physicochemical properties, and volatile aroma compound concentrations of 'Beibinghong' ice wine under different SO2 additions and constructed a fingerprint of volatile compounds in ice wine. The results showed that 44 typical volatile compounds in 'Beibinghong' ice wine were identified and quantified. The OAV and VIP values were calculated using the threshold values of each volatile compound, and t the effect of SO2 on the volatile compounds of 'Beibinghong' ice wine might be related to five aroma compounds: ethyl butyrate, ethyl propionate, ethyl 3-methyl butyrate-M, ethyl 3-methyl butyrate-D, and 3-methyl butyraldehyde. Tasting of 'Beibinghong' ice wine at different SO2 additions revealed that the overall flavor of 'Beibinghong' ice wine was the highest at an SO2 addition level of 30 mg/L. An SO2 addition level of 30 mg/L was the optimal addition level. The results of this study are of great significance for understanding the effect of SO2 on the fermentation of 'Beibinghong' ice wine. [ABSTRACT FROM AUTHOR]

Schisandra chinensis, as a traditional Chinese herbal medicine, has clear pharmacological effects such as treating asthma, protecting nerves and blood vessels, and having anti-inflammatory properties. Although the Schisandra chinensis fruit contain multiple active components, the lignans have been widely studied as the primary pharmacologically active compound. The volatile chemical components of Schisandra chinensis include a large amount of terpenes, which have been proven to have broad pharmacological activities. However, when to harvest to ensure the highest accumulation of pharmacologically active components in Schisandra chinensis fruits is a critical issue. The Schisandra chinensis fruit trees in the resource nursery were all planted in 2019 and began bearing fruit in 2021. Their nutritional status and tree vigor remain consistently good. The content of lignans and organic acids in the fruits of Schisandra chinensis over seven different harvest periods was tested, and the results of high-performance liquid chromatography (HPLC) indicated that the lignan content was higher, at 35 mg/g, in late July, and the organic acid content was higher, at 72.34 mg/g, in early September. If lignans and organic acids are to be selected as raw materials for pharmacological development, the harvest can be carried out at this stage. Using HS-GC-IMS technology, a total of 67 volatile flavor substances were detected, and the fingerprint of the volatile flavor substances in the different picking periods was established. It was shown by the results that the content of volatile flavor substances was the highest in early August, and 16 flavor substances were selected by odor activity value (OAV). The variable importance in projection (VIP) values of 16 substances were further screened, and terpinolene was identified as the key volatile flavor substance that caused the aroma characteristics of Schisandra chinensis fruit at different harvesting periods. If the aroma component content of Schisandra chinensis fruit is planned to be used as raw material for development and utilization, then early August, when the aroma component content is higher, should be chosen as the time for harvest. This study provides a theoretical basis for the suitable harvesting time of Schisandra chinensis for different uses, and promotes the high-quality development of the Schisandra chinensis industry. [ABSTRACT FROM AUTHOR]

As a typical and special type of urban setting, the university campus usually faces similar challenges as cities raised by high-density inhabitants. The smart campus has been introduced based on the smart city, as concepts, technologies, and solutions to improve livability and energy efficiency. Inhabitants' occupancy in buildings and open spaces on campus is critical to optimize campus management and services. Information about spatial occupancy of campus inhabitants can be produced based on various location-based solutions, such as global navigation satellite systems (GNSS), campus cameras, Bluetooth, and Wi-Fi. As an essential component in campus information infrastructure, Wi-Fi network covers almost the entire university campus and has advantages in collecting locations of campus inhabitants. In this paper, geo-visualization of spatial occupancy of campus inhabitants is designed and implemented using anonymized Wi-Fi network log data. First, 3-dimension building models are reconstructed based on LiDAR point clouds and construction drawings. Then, the Wi-Fi network log data are cleaned and preprocessed. Campus inhabitants' locations are extracted from structural Wi-Fi data. Geo-visualization at room, floor, and building levels is designed and implemented. On a temporal dimension, spatial occupancy can be visualized by each second, minute, hour, or day of the week in 3D buildings. The implementation of the geo-visualization is based on CesiumJS, which offers an interface for 3D-animated visualization and interaction. The research can be used to support university management and educators to implement the smart campus and optimize pedagogical research. [ABSTRACT FROM AUTHOR]

To study the evolution of geological hazard sources of waste dump slopes under rainfall conditions, a physical model of a rainfall-affected slope was designed. The apparent resistivity of the slope rock and soil mass at different rainfall times was measured via the high-density resistivity method, and the formation process of internal disaster sources of the rainfall-affected slope was obtained. The variation characteristics of the resistivity of the rain-affected slope were analyzed when it had a weak surface and crack development. Based on the three-water model and Maxwell conductivity formula, the evolution process of geological hazard sources of the rainfall-affected slope was summarized. A resistivity response mechanism equation for rainfall-induced slope hazard sources was derived and compared to the Archie formula, verifying the model rationality. The test results showed that the behavior of the rainfall-affected slope conforms to the saturated–unsaturated dynamic cycle process. The apparent resistivity was positively correlated with the development of slope pores and cracks and negatively correlated with the water content in the slope. The apparent resistivity increased during fracture development and decreased during water seepage. In the slope failure and disaster process, the apparent resistivity varies under the coupling effect of crack development and water seepage. During the formation of geological hazard sources, the apparent resistivity abruptly changes and fluctuates. Therefore, according to the abrupt changes and abnormal fluctuations in the apparent resistivity detected, the development of geological hazard sources of slopes can be determined. [ABSTRACT FROM AUTHOR]

Biocement can be achieved through the microbially induced carbonate precipitation (MICP) process. Such a method can potentially be utilized as an eco-friendly method for civil and environmental engineering applications such as soil ground improvement and wind erosion control of surface soil. In this method, one key step is the effective production of ureolytic bacteria. In previous laboratory and field studies, the cultivation and production of the bacteria used for the MICP were usually expensive and time-consuming. The purpose of this study was to optimize the cultivation method of the ureolytic bacteria (Sporosarcina pasteurii), and soil stabilization tests were conducted to verify the effectiveness of the cultured bacteria used to strengthen soil against the wind-induced erosion. Bacterial cultivation methods were studied by investigating the effects of different cultivation media and conditions. Testing variables included the types and concentrations of nitrogen sources (urea or NH4Cl), pH values (7.5–9.5), cultivation conditions (batch or chemostat condition), and different carbon sources. It was found that, with the same amount of nitrogen source, the test with pure urea had the highest biomass yield, urease activity, and specific urease activity than the other tests with pure NH4Cl or both NH4Cl and urea. The use of urea as the nitrogen source in the media also led to an increase in pH, which was not found in the test with pure NH4Cl. As for the factor of urea concentration, the tests with a higher urea concentration had a higher biomass yield, urease activity, and pH. The factor of pH values also played an important role. The test with an 8.5 initial pH value had a higher biomass yield, urease activity, and specific urease activity than the tests with 7.5 and 9.5 initial pH values. In the chemostat condition, the ureolytic bacteria could be effectively produced with urease activity up to 7 mmol/L/min, as compared with around 12 mmol/L/min activity in the batch condition. Thus, the optimum nitrogen source, pH value, and cultivation condition for the cultivation of Sporosarcina pasteurii was urea, 8.5, and batch condition, respectively. In addition, when soybean milk powder or milk powder was used as the carbon source, the urease activity was around 2.5 mmol/L/min, which is also high enough to be used for biocement. [ABSTRACT FROM AUTHOR]

The selector is an indispensable section of the phase change memory (PCM) chip, where it not only suppresses the crosstalk, but also provides high on-current to melt the incorporated phase change material. In fact, the ovonic threshold switching (OTS) selector is utilized in 3D stacking PCM chips by virtue of its high scalability and driving capability. In this paper, the influence of Si concentration on the electrical properties of Si-Te OTS materials is studied; the threshold voltage and leakage current remain basically unchanged with the decrease in electrode diameter. Meanwhile, the on-current density (Jon) increases significantly as the device is scaling down, and 25 MA/cm2 on-current density is achieved in the 60-nm SiTe device. In addition, we also determine the state of the Si-Te OTS layer and preliminarily obtain the approximate band structure, from which we infer that the conduction mechanism conforms to the Poole-Frenkel (PF) model. [ABSTRACT FROM AUTHOR]

China has developed a preliminary policy system for the development of new energy vehicles regarding the law, electricity price, grid-connected standards, project management, and financial support, however, defects remain in the policy and market environment, market mechanism, control technology, infrastructure, etc. We analyze new energy vehicles based on the analysis of basic data such as the number of electric vehicles and charging facilities, focusing on industrial development strategies, related subsidies, and tax policies. First, this paper summarizes the development status of China's new energy vehicles in different scenarios. In 2021, China's new energy vehicle production was 3545 thousand, and sales amounted to 3521 thousand. According to preliminary estimates, the number of new energy vehicles will exceed 15 million in 2030. The research route for the development of new energy vehicle bottlenecks is proposed. Secondly, we analyze foreign and Chinese policies on different stages and construct the implementation path for the healthy and stable development of China's new energy vehicles. By comparing the basic indicators, related policies, and related innovation activities of new energy vehicles in China, we conclude that the development of the national electric vehicle industry needs to be increased in terms of government policies, business model innovation, and public awareness. [ABSTRACT FROM AUTHOR]

Currently, waterborne coatings are widely used, but their poor anticorrosion performance compared with solvent-based coatings hinders their further application. In this study, a series of Zn5Mo2O11·5H2O@sulfonated graphene (SZMO@SG) composites are prepared using the template method. SZMO@SG is a type of two-dimensional material with a large diameter and low thickness compared to traditional zinc molybdate. Combining SZMO@SG with waterborne hydroxy acrylic latex and isocyanate, the polyurethane coatings with 3 wt% SZMO@SG achieve the best anticorrosion performance in the 3.5 wt% NaCl immersion test. In electrochemical impedance spectroscopy (EIS), the charge transfer resistance (Rct) of polyurethane coatings with 3 wt% SZMO@SG reaches 283,100 Ω·cm2 after soaking in a 3.5 wt% NaCl solution for 48 h. Its corrosion potential and corrosion current are only −0.453 V and 1.71 × 10−3 mA/cm2, respectively. After metal substrate corrosion, the metal substrate of polyurethane coatings with SZMO@SG contain more Mo, Zn and low-state Fe atoms. It confirms that a passivation film was deposited on the metal substrate and prevents Fe from oxidation. The elemental content and distribution of the coatings after the NaCl solution immersion test indicate that SZMO@SG may have the Na+ adsorption function. [ABSTRACT FROM AUTHOR]

As the core component of agricultural development projects, high-standard farmland construction is a reliable measure of agricultural production, and can be used to improve the economy in rural areas. Based on provincial panel data, this paper adopts the continuous difference-in-differences (DID) method to analyze the impact of China's high-standard basic farmland construction policy on the incidence of rural poverty and its mechanisms. The results show that this policy can significantly reduce the incidence of rural poverty by 7.4%, and if, after using robust standard error and bootstrap sampling 1000 times for a robustness test, the regression results are still robust, this also shows that this inhibitory effect is stable and persistent. It can be seen from a heterogeneity analysis that the implementation of the policy has a more significant effect on poverty reduction in areas with a higher incidence of rural poverty and a larger scale of land remediation, as well as areas in the eastern and western regions. A mechanism analysis shows that natural disasters, output value and technological progress play a partial intermediary role in the poverty reduction effects of high-standard basic farmland construction policy, and the intermediary effects are 5.79%, 44.03%, and 14.13%, respectively. This paper suggests that we should continue to promote the construction of high-standard basic farmland, explore suitable construction modes of high-standard basic farmland for different regions, continuously promote the process of agricultural modernization, and broaden the ways through which rural residents are able to accumulate capital to promote rural poverty reduction and revitalization. [ABSTRACT FROM AUTHOR]

Simple Summary: SAR324, newly proposed as its own candidate phylum, is a diverse and globally abundant bacterial group living in a wide range of environments, from deep-sea hydrothermal vents and brine pools to the epipelagic regions of the global oceans and terrestrial aquifers. The different SAR324 clades harbor a diverse array of genes and pathways well adapted to their respective environments. This metabolic flexibility explains the ubiquitous presence and the importance of SAR324 in global biogeochemical cycles. The bacterial SAR324 cluster is ubiquitous and abundant in the ocean, especially around hydrothermal vents and in the deep sea, where it can account for up to 30% of the whole bacterial community. According to a new taxonomy generated using multiple universal protein-coding genes (instead of the previously used 16S rRNA single gene marker), the former Deltaproteobacteria cluster SAR324 has been classified since 2018 as its own phylum. Yet, very little is known about its phylogeny and metabolic potential. We downloaded all publicly available SAR324 genomes (65) from all natural environments and reconstructed 18 new genomes using publicly available oceanic metagenomic data and unpublished data from the waters underneath the Ross Ice Shelf. We calculated a global SAR324 phylogenetic tree and identified six clusters (namely 1A, 1B, 2A, 2B, 2C and 2D) within this clade. Genome annotation and metatranscriptome read mapping showed that SAR324 clades possess a flexible array of genes suited for survival in various environments. Clades 2A and 2C are mostly present in the surface mesopelagic layers of global oceans, while clade 2D dominates in deeper regions. Our results show that SAR324 has a very versatile and broad metabolic potential, including many heterotrophic, but also autotrophic pathways. While one surface water associated clade (2A) seems to use proteorhodopsin to gain energy from solar radiation, some deep-sea genomes from clade 2D contain the complete Calvin–Benson–Bassham cycle gene repertoire to fix carbon. This, in addition to a variety of other genes and pathways for both oxic (e.g., dimethylsulfoniopropionate degradation) and anoxic (e.g., dissimilatory sulfate reduction, anaerobic benzoate degradation) conditions, can help explain the ubiquitous presence of SAR324 in aquatic habitats. [ABSTRACT FROM AUTHOR]

As sessile organisms, plants constantly face challenges from the external environment. In order to meet these challenges and survive, plants have evolved a set of sophisticated adaptation strategies, including changes in leaf morphology and epidermal cell development. These developmental patterns are regulated by both light and hormonal signaling pathways. However, our mechanistic understanding of the role of these signaling pathways in regulating plant response to environmental stress is still very limited. By applying single-cell RNA-Seq, we determined the expression pattern of PHYTOCHROME INTERACTING FACTOR (PIF) 1, PIF3, PIF4, and PIF5 genes in leaf epidermal pavement cells (PCs) and guard cells (GCs). PCs and GCs are very sensitive to environmental stress, and our previous research suggests that these PIFs may be involved in regulating the development of PCs, GCs, and leaf morphology under environmental stress. Growth analysis showed that pif1/3/4/5 quadruple mutant maintained tolerance to drought and salt stress, and the length to width ratio of leaves and petiole length under normal growth conditions were similar to those of wild-type (WT) plants under drought and salt treatment. Analysis of the developmental patterns of PCs and GCs, and whole leaf morphology, further confirmed that these PIFs may be involved in mediating the development of epidermal cells under drought and salt stress, likely by regulating the expression of MUTE and TOO MANY MOUTHS (TMM) genes. These results provide new insights into the molecular mechanism of plant adaptation to adverse growth environments. [ABSTRACT FROM AUTHOR]

Surface-enhanced Raman scattering (SERS) for semiconductor nanomaterial systems is limited due to weak Raman signal intensity and unclear charge-transfer (CT) processes for chemical enhancement. Here, rare-earth element neodymium-doped titanium dioxide (Nd-TiO2) nanoparticles (NPs) were synthesized by the sol–gel method. The characterizations show that the doping of Nd ions causes TiO2 NPs to show an increase in the concentration of defects and change in the energy level structure. The CT process between Nd-TiO2 NPs substrate and probe molecule 4-Mercaptopyridine (4-Mpy) was innovatively analyzed using the relative energy level location relationship of the Dorenbos model. The SERS signal intensity exhibits an exponential enhancement with increasing Nd doping concentration and reaches its optimum at 2%, which is attributed to two factors: (1) The increase in the defect concentration is beneficial to the CT process between the TiO2 and the probe molecule; (2) the introduction of 4f electron orbital energy levels of rare-earth ions created unique CT process between Nd3+ and 4-Mpy. Moreover, the Nd-TiO2 NPs substrate shows excellent SERS performance in Raman signal reproducibility (RSD = 5.31%), the limit of detection (LOD = 10−6 M), and enhancement factor (EF = 3.79 × 104). Our work not only improves the SERS performance of semiconductor substrates but also provides a novel approach to the development of selective detection of probe molecules. [ABSTRACT FROM AUTHOR]

This research focuses on a desensitization method to develop a wide-range FBG sensor for extra-large strain monitoring, which is an essential requirement in large scale infrastructures or for some special occasions. Under appropriate hypotheses, the strain transfer distribution of wide-range FBG sensor based on the shear-lag theory is conducted to improve the accuracy of extra-large strain measurements. It is also discussed how the elastic modulus of adhesive layer affects the strain transfer rate. Two prototypes in different monitoring ranges are designed and fabricated by two layers of steel pipe encapsulation. The presented theoretical model is verified by experimental results. Moreover, it is demonstrated that experimentation in regards to the calibration of the wide-range FBG sensor, improved the amplification coefficient up to 2.08 times and 3.88 times, respectively. The static errors are both calculated and analyzed in this experiment. The wide-range FBG strain sensor shows favourable linearity and stability, which is an excellent property of sensors for extra-large strain monitoring. [ABSTRACT FROM AUTHOR]