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China and Brazil established the "Comprehensive Strategic Partnership" in 2012. Since the establishment of the partnership, China and Brazil have continued to deepen economic and trade cooperation, and cooperation in various fields has achieved remarkable results. China has long been consistent in maintaining its status as Brazil's largest export destination and source of imports. The total volume of bilateral trade between China and Brazil has significantly increased, and the trade structure in goods is becoming increasingly diversified. The total volume of bilateral trade between China and Brazil has grown considerably, and the trade structure in goods has become increasingly diversified. On 20 April 2023, when Brazilian President Lula visited China, China and Brazil issued a joint statement on deepening the "Comprehensive Strategic Partnership", further deepening the cooperation in trade in goods has become a consensus. From the perspective of goods trade under the comprehensive strategic partnership, the article analyses the complementarities and competitiveness of goods trade between China and Brazil with the help of relevant formulas to form a complete and transparent study of the goods trade between the two countries. [ABSTRACT FROM AUTHOR]

The sustainable development of urban transport is the key to sustainable urban development. This study analyses and identifies the evaluation indicators affecting Macau's transport, and analyses the relationship between the relevant evaluation indicators and passenger satisfaction, thus contributing to the improvement and the sustainable development of Macau's public transport services. This study uses a questionnaire to collect data. Based on the current situation of Macau's social groups, the mainland students in Macau who were selected as the target group are significantly different from residents and tourists in Macau; their needs and perceptions of public transport are more intense. This group's needs and perceptions of public transport are significantly different from those of residents and tourists. The set indicators of satisfaction with public transport in Macau reflect them. After analyzing and determining the evaluation indicators, structural equation modeling was applied to establish the structural models of Macau's three public transport services. Finally, the shortcomings of the three public transport services were reflected through the standardized coefficient weights and passenger satisfaction. The study results show that the seating arrangement and smoothness of travel of Macau's public transport services need to be improved, and the dial-a-ride service's price setting and waiting time need to be improved. The price setting, convenience of hailing, and payment method of the taxi service need to be improved. In contrast, overall, the satisfaction level of the dial-a-ride service is significantly higher than that of the taxi service. Therefore, the findings of the study have important policy implications for relevant policymakers, who should not only focus on optimizing daily operations but, more importantly, on improving the shortcomings mentioned above, which are of great value in enhancing the passengers' perception of public transport in Macau and are also crucial to achieving the sustainable development of public transport in Macau. [ABSTRACT FROM AUTHOR]

The sensitive quantification of low-abundance nucleic acids holds importance for a range of clinical applications and biological studies. In this study, we describe a facile microfluidic chip for absolute DNA quantifications based on the digital loop-mediated isothermal amplification (digital LAMP) method. This microfluidic chip integrates a cross-flow channel for droplet generation with a micro-cavity for droplet tiling. DNA templates in the LAMP reagent were divided into ~20,000 water-in-oil droplets at the cross-flow channel. The droplets were then tiled in the micro-cavity for isothermal amplification and fluorescent detection. Different from the existing polydimethylsiloxane (PDMS) microfluidic chips, this study incorporates gold nanoparticles (AuNPs) into PDMS substrate through silica coating and dodecanol modification. The digital LAMP chip prepared by AuNPs-PDMS combines the benefits of the microstructure manufacturing performance of PDMS with the light-to-heat conversion advantages of AuNPs. Upon illumination with a near infrared (NIR) LED, the droplets were stably and efficiently heated by the AuNPs in PDMS. We further introduce an integrated device with a NIR heating unit and a fluorescent detection unit. The system could detect HBV (hepatitis B virus)-DNA at a concentration of 1 × 101 to 1 × 104 copies/μL. The LED-driven digital LAMP chip and the integrated device; therefore, demonstrate high accuracy and excellent performance for the absolute quantification of low-abundance nucleic acids, showing the advantages of integration, miniaturization, cost, and power consumption. [ABSTRACT FROM AUTHOR]