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Oil tea camellia (Camellia spp.) is an important woody oil crop with a high nutritional and economic value. Whole-genome resequencing (WGR) technology can provide an in-depth understanding of the genetic background of this plant as well as a reference for breeding research, germplasm resource conservation, and genetic modification. In this study, we analyzed SNP and InDel variations in 49 individual oil tea camellia germplasm samples collected from five populations located in three provinces of China: Hainan, Guangdong and Guangxi. The samples were analyzed through WGR after the ploidy of the samples was determined through flow cytometry. A total of 239,441,603 high-quality single nucleotide polymorphisms (SNPs) and 23,510,374 high-quality insertion/deletion variation sites (InDels) were obtained. The distribution of SNPs and InDels in different functional regions differed significantly, with a high density of variations in non-coding regions, such as intergenic regions and introns, and a relatively low density of variations in coding regions. Transition was the main type of SNP variation. A population genetic diversity analysis revealed that the sampled oil tea camellia populations exhibited a high genetic diversity and extensive genetic variation. The genetic diversity of the oil tea camellia populations in the Hainan region was higher than inland regions. This study also determined the genetic diversity of and variations between octoploid and decaploid oil tea camellia in the tropics and between Hainan-based and inland oil tea camellia. Such findings provide a reference for the conservation of germplasm resources and the genetic modification of oil tea camellia. [ABSTRACT FROM AUTHOR]

Horseradish peroxidase (HRP) combined with its fluorescence substrates is attracting increasing attention for biochemical analysis. Amplex red is the most widely used fluorescence substrate to HRP; however, it suffers from some drawbacks, such as nonspecific responsiveness toward carboxylesterases. Discovering a new small molecular fluorescence substrate with improved sensitivity and selectivity for HRP is thus desired. Herein, three dihydrofluorescein derivatives (DCFHs) are presented to serve as HRP substrates through fluorescence turn-on methods. The most promising one, 2,7-dichloro-9-(2-(hydroxymethyl)phenyl)-9H-xanthene-3,6-diol (DCFH-1), exhibited excellent sensitivity in the detection of HRP. Moreover, DCFH-1 does not respond to carboxylesterase, thus holding advantages over Amplex red. In the further study, the detection reagent in the commercial ELISA kits was replaced with DCFH-1 to establish a new fluorescence ELISA, which works very well in the quantification of inflammatory cytokine biomarkers from in vitro models. [ABSTRACT FROM AUTHOR]

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a secretory serine protease synthesized primarily by the liver. It mainly promotes the degradation of low-density lipoprotein receptor (LDL-R) by binding LDL-R, reducing low-density lipoprotein cholesterol (LDL-C) clearance. In addition to regulating LDL-R, PCSK9 inhibitors can also bind Toll-like receptors (TLRs), scavenger receptor B (SR-B/CD36), low-density lipoprotein receptor-related protein 1 (LRP1), apolipoprotein E receptor-2 (ApoER2) and very-low-density lipoprotein receptor (VLDL-R) reducing the lipoprotein concentration and slowing thrombosis. In addition to cardiovascular diseases, PCSK9 is also used in pancreatic cancer, sepsis, and Parkinson's disease. Currently marketed PCSK9 inhibitors include alirocumab, evolocumab, and inclisiran, as well as small molecules, nucleic acid drugs, and vaccines under development. This review systematically summarized the application, preclinical studies, safety, mechanism of action, and latest research progress of PCSK9 inhibitors, aiming to provide ideas for the drug research and development and the clinical application of PCSK9 in cardiovascular diseases and expand its application in other diseases. [ABSTRACT FROM AUTHOR]

The Pinghu Formation consists primarily of marine-continental transitional deposits. The widely distributed fluvial and tidal transgressive sand bodies comprise the main reservoirs of the Baochu slope zone in the Xihu Sag in the East China Sea Shelf Basin. These sand bodies are deeply buried, laterally discontinuous, and are frequently interrupted by coal-bearing intervals, thereby making it extremely difficult for us to characterize their hydrocarbon potential quantitatively via seismic inversion techniques, such as multi-attribute seismic analysis and post-stack seismic inversion, hindering further hydrocarbon exploration in the Xihu Sag. Here, a prestack seismic inversion approach is applied to the regional seismic data to decipher the spatiotemporal distribution pattern of the sand bodies across the four sequences, i.e., SQ1, SQ2, SQ3, and SQ4, from bottom up, within the Pinghu Formation. In combination with detailed petrology, well log, and seismic facies analysis, the secular evolution of the sedimentary facies distribution pattern during the accumulation of the Pinghu Formation is derived from the sand body prediction results. It is concluded that the sedimentary facies and sand body distribution pattern rely on the interplay between the hydrodynamics of fluvial and tidal driving forces from the continent and open ocean, respectively. Drops in the sea level led to the gradual weakening of tidal driving forces and relative increases in riverine driving forces. The direction of the sand body distribution pattern evolves from NE–SW oriented to NW–SE oriented, and the dominant sand body changes from tidal facies to fluvial facies. In addition, the sea level drop led to the decrease in the water column salinity, redox condition, organic matter composition, and the development of coal seams, all of which directly influenced the quality of reservoir and source rocks. The sand bodies in SQ2 and SQ3 are favorable reservoirs in the Pinghu Formation due to their good reservoir properties and great thickness. The high-quality source rock in SQ1 could provide significant hydrocarbons and get preserved in the sand body within SQ2 and SQ3. This contribution provides an insight into the control of the sea level change over the development of hydrocarbon reservoirs in the petroleum system from marginal-marine environments such as the Xihu Sag. [ABSTRACT FROM AUTHOR]

Titanium dioxide is a mainstream photocatalyst, but it still confronts great obstacles of poor visible light absorption and rapid recombination rate of photogenerated carriers. Herein, we describe the design of a highly active visible-light photocatalytic system of graphited carbon layers anchored V2O5/TiO2 heterojunctions derived from Ti3C2 MXene, which demonstrates about 4.58 and 2.79 times higher degradation activity of MB under visible light (λ > 420 nm) than pure V2O5 and TiO2-carbon. Combined with the characterization results, the formed V2O5/TiO2 heterojunction promotes the separation of photogenerated carriers, while the graphitized carbon derived from MXene acts as an electronic reservoir to enhance the absorption of visible light. The ESR results show that superoxide radicals and hydroxyl radicals are the main active species in the reaction system. Therefore, we propose a possible mechanism model to provide a feasible idea for the subsequent design of high-efficiency photocatalysts for environmental treatment. [ABSTRACT FROM AUTHOR]

Acute myocardial infarction is a major global health problem, and the repair of damaged myocardium is still a major challenge. Myocardial injury triggers an inflammatory response: immune cells infiltrate into the myocardium while activating myofibroblasts and vascular endothelial cells, promoting tissue repair and scar formation. Fragments released by cardiomyocytes become endogenous "danger signals", which are recognized by cardiac pattern recognition receptors, activate resident cardiac immune cells, release thrombin factors and inflammatory mediators, and trigger severe inflammatory responses. Inflammatory signaling plays an important role in the dilation and fibrosis remodeling of the infarcted heart, and is a key event driving the pathogenesis of post-infarct heart failure. At present, there is no effective way to reverse the inflammatory microenvironment in injured myocardium, so it is urgent to find new therapeutic and diagnostic strategies. Nanomedicine, the application of nanoparticles for the prevention, treatment, and imaging of disease, has produced a number of promising applications. This review discusses the treatment and challenges of myocardial injury and describes the advantages of functional nanoparticles in regulating the myocardial inflammatory microenvironment and overcoming side effects. In addition, the role of inflammatory signals in regulating the repair and remodeling of infarcted hearts is discussed, and specific therapeutic targets are identified to provide new therapeutic ideas for the treatment of myocardial injury. [ABSTRACT FROM AUTHOR]

Motivated by applications in topographic map information extraction, our goal was to discover a practical method for scanned topographic map (STM) segmentation. We present an advanced guided watershed transform (AGWT) to generate superpixels on STM. AGWT utilizes the information from both linear and area elements to modify detected boundary maps and sequentially achieve superpixels based on the watershed transform. With achieving an average of 0.06 on under-segmentation error, 0.96 on boundary recall, and 0.95 on boundary precision, it has been proven to have strong ability in boundary adherence, with fewer over-segmentation issues. Based on AGWT, a benchmark for STM segmentation based on superpixels and a shallow convolutional neural network (SCNN), termed SSCNN, is proposed. There are several notable ideas behind the proposed approach. Superpixels are employed to overcome the false color and color aliasing problems that exist in STMs. The unification method of random selection facilitates sufficient training data with little manual labeling while keeping the potential color information of each geographic element. Moreover, with the small number of parameters, SCNN can accurately and efficiently classify those unified pixel sequences. The experiments show that SSCNN achieves an overall F1 score of 0.73 on our STM testing dataset. They also show the quality of the segmentation results and the short run time of this approach, which makes it applicable to full-size maps. [ABSTRACT FROM AUTHOR]

Dalbergia odorifera, a traditional Chinese medicine, has been used to treat cardio- and cerebrovascular diseases in China for thousands of years. Flavonoids are major active compounds in D. odorifera. In this paper, a rapid and sensitive ultra-high performance liquid chromatography-triple quadrupole mass spectrometry method was developed and validated for simultaneous determination of 17 flavonoids in D. odorifera. Quantification was performed by multiple reaction monitoring using electrospray ionization in negative ion mode. Under the optimum conditions, calibration curves for the 17 analytes displayed good linearity (r2 > 0.9980). The intra- and inter-day precisions (relative standard deviations) were lower than 5.0%. The limit of quantitation ranged from 0.256 to 18.840 ng/mL. The mean recovery range at three spiked concentrations was 94.18–101.97%. The validated approach was successfully applied to 18 samples of D. odorifera. Large variation was observed for the contents of the 17 analytes. Sativanone and 3′-O-methylviolanone were the dominant compounds. The fragmentation behaviors of six flavonoids were investigated using UPLC with quadrupole time-of-flight tandem mass spectrometry. In negative ion electrospray ionization mass spectrometry, all the flavonoids yielded prominent [M − H]− ions. Fragments for losses of CH3, CO, and CO2 were observed in the mass spectra. Formononetin, liquiritigenin, isoliquiritigenin, sativanone, and alpinetin underwent retro-Diels–Alder fragmentations. The proposed method will be helpful for quality control of D. odorifera. [ABSTRACT FROM AUTHOR]