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This study investigates the impact of water faecal contamination on highly threatened European brook lamprey larvae (Lampetra planeri). Water samples and the midgut contents of lampreys collected from a small lowland river upstream (site 1) and downstream from a wastewater treatment plant (WWTP) discharge (site 2) were analysed to check how the faecal microbial load of the habitat is reflected in the intestines of larval lampreys. The counts of viable mesophiles, psychrophiles, Escherichia coli and faecal streptococci as bacterial indicators of microbial (including faecal) water contamination were estimated. Microbial composition and abundance in larval midgut contents depended on the numbers of various microorganisms in the water environment. At site 2, the water was heavily microbiologically contaminated throughout the year by sewage inflow from the WWTP, and the amounts of studied bacteria were also high in the midgut of lampreys inhabiting site 2 regardless of the season. At site 1, water quality was better, and the levels of tested microbial indicators were lower in the intestines of the lampreys living there. The numbers of bacteria dependent on water temperature were growing in warmer seasons both in water and in intestines. Sewage pollution negatively influenced the condition of lampreys in site 2, where they exhibited lower body condition than in site 1. [ABSTRACT FROM AUTHOR]

Simple Summary: Gentle touch has been repeatedly shown to have a positive effect on livestock. The purpose of this study was to determine potential changes in physiological indicators in lambs subjected to gentle stroking. A total of 40 lambs of two breeds were assigned to two control groups (n = 20) and two experimental groups (n = 20). Lambs from the experimental groups received treatment (the gentle stroking procedure). Analyses consisted of determining the following parameters: heart rate (HR), saturation (S) and biochemical analysis. An increase in cortisol levels and a decrease in total protein levels indicate a disruption of homeostasis. At the same time, a significant increase in glycogen and a significant decrease in glucose and heart rate values suggest reduced stress levels. The results obtained in the experimental groups suggest that interactions with humans, even of a positive nature, can be a source of stress, but this is a transient and adaptive reaction. Interactions with humans can be a source of stress, but long-term and positive ones will lead to positive changes and easier handling of animals. The aim of the study was to determine changes in physiological indicators in lambs subjected to gentle stroking. The study included 40 three-week-old lambs (20 females of the synthetic prolific-meat (BCP) line and 20 females of the Świniarka (SW) breed). The animals were assigned to two control groups (n = 20) and two experimental groups (n = 20). Lambs from the experimental groups received treatment. Analyses consisted of determining the following parameters: heart rate (HR), saturation (S) and biochemical analysis. In the groups of sheep subjected to gentle massage, the analyses revealed a significant increase in the levels of cortisol, CAT, GST, GPx, among others, and a significant decrease in the levels of total protein, SOD, TAC, uric acid and Na+. At the same time, indicators of reduced stress levels were revealed, i.e., a significant increase in glycogen levels and a significant decrease in glucose and HR values. These results suggest that the introduction of gentle touch can induce positive states in lambs, and that the stress response may be transient and adaptive. Nevertheless, it is important to note that these interactions can be a source of stress, even if the behavioral response does not necessarily indicate stress. [ABSTRACT FROM AUTHOR]

Simple Summary: For the last decade, capons have become very popular because of the specific quality traits of their meat. In case of laying hens caponization may be a solution to the problem of redundant roosters. The Greenleg partridge breed, covered by a program for livestock genetic resources conservation in Poland, consisted of attraction, as the oldest Polish hens breed, as well as the source of poultry products (eggs and meat) in a small farm. The cockerels are often intended for caponization, due to Polish tradition and consumer preferences. However, this procedure influences not only their productivity or meat quality, but also physiological aspect of birds. We were interested not only in capons compared to roosters, but also in relation between sexes in case of productive traits as well as their selected physiological traits. The sex effect was found in all experimental aspects. Cocks had the highest carcass yield, however, the biggest breast muscles proportion were stated in capons carcasses. The highest proportion of abdominal fat pad was found in females but the lack of sex hormones in capons also contributed to a higher fat accumulation. The serum profile showed that the sexual maturity of hens increased lipids content (cholesterol, trigliceroles) caused by laying production. The aim of the study was to assess the influence of sex, including caponization, on selected physiological and productive traits of Greenleg partridge (GP) birds. The study material consisted of 120 GP chicks (40 females and 80 males), divided into 3 equal groups (4 replication in each) and kept in litter system and fed ad libitum. A total of 40 cocks have been surgically castrated. The body weight (BW) of birds were measured biweekly. At the age of 24 weeks 8 birds/group were slaughtered, their carcasses were subjected to simplified dissection. Blood samples were collected and among others biochemical profile of serum was established. The lowest BW, regardless of age, had hens. From 18th week capons had the highest BW and finally it was similar to cocks. Cocks demonstrated, significantly, the highest carcass yield, however, the biggest proportion of breast muscles were stated in capons carcasses. The effect of sex is very clear in case of abdominal fat pad. The highest proportion of it was found in females but the lack of sex hormones in capons also contributed to a higher fat accumulation. The serum profile showed that the sexual maturity of hens increased lipids content (cholesterol, trigliceroles) caused by laying production. [ABSTRACT FROM AUTHOR]

Simple Summary: The Polish Greyhound is an old Polish breed. The first descriptions of hunting with greyhounds can be found in the hunting literature of the 16th century, and the first detailed description of a greyhound comes from 1600. The Polish Greyhound probably originates from the Saluki-type Asian greyhounds. The population of this breed is very small and limited mainly to the territory of its country of origin (Poland). Pedigree books were opened for this breed in 1981. The narrow gene pool necessitated mating relatives, resulting in the appearance of various genetic diseases in the breed. The analysis of Polish Greyhound pedigrees registered in the pedigree books from the time of the registration of the breed and molecular tests facilitated calculation of the degree of relatedness in the breed and to design future breeding plans, taking into account that a 2.56% increase in inbreeding per complete generation will be observed if the current breeding program and similar matings are maintained. The aim of the study was to assess the genetic variability of the Polish Greyhound population based on pedigree analysis and molecular DNA testing and to determine the degree of relatedness among individuals in the population. Pedigree data of 912 Polish Greyhounds recorded in pedigree books since they were opened for this breed were analyzed. For molecular testing, DNA was obtained from cheek swabs taken from 235 dogs of the tested breed. A panel of 21 markers (Short Tandem Repeat—STR) was used. The mean inbreeding determined for the Polish Greyhound population based on pedigree analyses was low and amounted to 11.8%, but as many as 872 individuals of the 912 dogs in the studied population were inbred. A total of 83 founders (at least one unknown parent) were identified, among which 27 founders had both unknown parents. Full-sibling groups consisted of 130 individuals, with a minimum and maximum litter size of 2 and 16, respectively. The average litter size was 5.969. Gene diversity calculated based on the mean kinship matrix was 0.862 and the population mean kinship was 0.138. The founder genome equivalent based on the mean kinship matrix was 3.61; the founder genome surviving level was 12.34; the mean Ne was estimated at 21.76; and the Ne/N ratio was 0.135. The FIS inbreeding coefficient for 21 STR was negative, and the mean FIS value for all loci had a low negative value (−0.018). These values suggest a low level of inbreeding in the examined breed as well as the avoidance of mating related animals. [ABSTRACT FROM AUTHOR]

Simple Summary: Flaxseed oils have long been used in animal feeding and human nutrition due to the beneficial effects of their biologically active substances, especially omega-3 polyunsaturated fatty acids (PUFA). This study aimed to evaluate the effect of the replacement of soybean oil (SO) with flaxseed oil (FO) in the horses' diet on biochemical, haematological, and redox status parameters of the blood. We found FO contributed to an improvement of lipid profile, haematological parameters of the blood and enhanced antioxidant defence mechanisms of horses. This suggests that substitution of SO with FO in the diet of horses is beneficial for their health status. This study compared the effect of two dietary vegetable oils on plasma biochemical indices, haematological parameters, and redox status of horses. Forty riding horses (20 mares and 20 stallions) of the Malopolski breed were divided equally into two groups that were similar in terms of age, sex, and body weight (on average 530 ± 30 kg). The horses received soybean oil (SO) or flaxseed oil (FO) in the amount of 25 mL per 100 kg BW/day. After 60 days, blood was collected for biochemical and haematological analyses. The results show that horses receiving FO as compared to the SO group had significantly lower plasma levels of glucose, low density lipoprotein cholesterol, total cholesterol/high-density lipoprotein (HDL) ratio and triacylglycerols, as well as the activities of alanine aminotransferase and alkaline phosphatase. In turn, %HDL-TC and lactate dehydrogenase activity were significantly higher in the FO group. The inclusion of FO in the diet contributed to an increase in antioxidant indices: creatinine, vitamin C, copper, and zinc contents and also superoxide dismutase and catalase activities. The level of the end product of lipid peroxidation, i.e., malonyl dialdehyde, in the FO group as compared to the SO group was significantly lower. Moreover, FO caused an elevation in red blood cell indicators, lymphocyte count and lysozymes. In conclusion, FO exerts a beneficial effect by stimulating antioxidant defence mechanisms of horses and reducing the severity of oxidative stress. FO also improved the lipid profile and haematological parameters of the blood. The replacement of SO by FO is recommended based on these findings. [ABSTRACT FROM AUTHOR]

Simple Summary: Environmental requirements ensuring behavioural welfare to laying hens may vary depending on the breed. Chickens representing various breeds and reared in the same environment were found not only to differ in the level of activity, emotional arousal, and degree of curiosity, but also to prefer different enrichments of the environment, which was reflected by different levels of stress in these birds. Hence, a question was posed whether the behavioural differences observed were innate behavioural patterns typical of the breed or whether they are an effect of the modifying impact of the environment, which varies between breeds. It has been hypothesised that differences observed already in chicks of different breeds may not be associated with the modifying effect of the environment. Instead, they may be a genetically determined breed-specific behaviour. The present investigations consisted in behavioural tests and assessment of the behaviour of chicks of three laying hen breeds. The study involved 60 green-legged partridge (Zk), 60 Polbar (Pb), and 60 Leghorn (Lg) chicks. The investigations have demonstrated that the birds from the analysed breeds exhibit behavioural differences already on the first days of life. The effect of the breed was evident in the case of such traits as strategy for acquisition of food resources, fearfulness/curiosity, and interest in elements of the environment. With age, chicks may exhibit changes in their emotions, e.g., more pronounced fearfulness, and environmental preferences. However, in the latter case, there is clear tendency towards breed-specific behaviours exhibited from the first days of life. The level of activity, which largely differentiates adult birds, does not discriminate between chicks. The aim of the study was to assess the behaviour of chicks of three different breeds of laying hens differing in the activity, emotional reactivity, and environmental preferences. Another objective was to answer the question whether the behavioural differences between adult birds would be evident already in the chick period or whether they are an effect of the further modifying impact of the environment. 60 green-legged partridge, 60 Polbar, and 60 Leghorn chicks were used in the experiments. The chicks hatched in a flock where hens were previously assessed with behavioural tests and the corticosterone levels in their feathers was determined, indicating significant differences in the temperament and stress level between the breeds. Five tests were carried out: two on competitiveness, activity, interest and fearfulness/curiosity. The experiments revealed considerable differences between the chicks. The Zk birds coped better with situations requiring swiftness and initiative. The Pb chicks were slower than Zk and Lg and did not make quick decisions. Hence, a lower number of these birds entering and leaving the test cage and staying inside was recorded. The Zk chicks exhibited a higher level of fearfulness than the other breeds. In terms of the environment enrichment elements, sand and woodchips were more attractive to the Zk chicks, whereas the Lg and Pb birds preferred pecking the string. No differences in the time of undertaking the analysed activities were found between the breeds. [ABSTRACT FROM AUTHOR]