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The genus Camphora encompasses species of significant ecological and economic importance, such as C. parthenoxylon and C. officinarum, which exhibit distinct phenotypic traits and stress responses. This study seeks to elucidate the molecular basis of cold tolerance through comparative transcriptomic analysis complemented by physiological characterization. RNA sequencing revealed 6123 differentially expressed genes between the two species, with enriched pathways related to cold stress, oxidative stress, carotenoid biosynthesis, and photosynthesis. Key genes, such as annexin D5, chlorophyll a/b-binding protein, early light-induced protein 1, 9-cis-epoxycarotenoid dioxygenase, were identified as critical regulators of frost resistance, photosynthetic efficiency, and carotenoid biosynthesis. Functional enrichment analyses highlighted the involvement of signal transduction, membrane stabilization, and secondary metabolism in adaptive responses. Physiological assays supported these findings, showing higher chlorophyll and carotenoid content and enhanced antioxidative enzyme activities in C. parthenoxylon. These results provide valuable insights into the genetic and biochemical mechanisms underlying stress adaptation in Camphora species and offer promising targets for enhancing resilience in economically valuable plants. [ABSTRACT FROM AUTHOR]

African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a severe hemorrhagic disease with a mortality rate reaching 100%. Despite extensive research on ASFV mechanisms, no safe and effective vaccines or antiviral treatments have been developed. Live attenuated vaccines generated via gene deletion are considered to be highly promising. We developed a novel recombinant ASFV strain by deleting MGF360-10L and MGF505-7R, significantly reducing virulence in pigs. In the inoculation experiment, pigs were infected with 104 50% hemadsorption doses (HAD50) of the mutant strain. All the animals survived the observation period without showing ASF-related clinical signs. Importantly, no significant viral infections were detected in the cohabitating pigs. In the virus challenge experiment, all pigs succumbed after being challenged with the parent strain. RNA-seq analysis showed that the recombinant virus induced slightly higher expression of natural immune factors than the parent ASFV; however, this level was insufficient to provide immune protection. In conclusion, our study demonstrates that deleting MGF360-10L and MGF505-7R from ASFV CN/GS/2018 significantly reduces virulence but fails to provide protection against the parent strain. [ABSTRACT FROM AUTHOR]

Ischemic stroke is associated with a high mortality rate, and effective treatment strategies are currently lacking. In this study, we aimed to develop a novel nano delivery system to treat ischemic stroke via intranasal administration. A three-factor Box–Behnken experimental design was used to optimize the formulation of liposomes co-loaded with Panax notoginseng saponins (PNSs) and Ginsenoside Rg3 (Rg3) (Lip-Rg3/PNS). Macrophage membranes were coated onto the surface of the optimized liposomes to target the ischemic site of the brain. The double-loaded liposomes disguised by macrophage membranes (MM-Lip-Rg3/PNS) were spherical, in a "shell–core" structure, with encapsulation rates of 81.41% (PNS) and 93.81% (Rg3), and showed good stability. In vitro, MM-Lip-Rg3/PNS was taken up by brain endothelial cells via the clathrin-dependent endocytosis and micropinocytosis pathways. Network pharmacology experiments predicted that MM-Lip-Rg3/PNS could regulate multiple signaling pathways and treat ischemic stroke by reducing apoptosis and inflammatory responses. After 14 days of treatment with MM-Lip-Rg3/PNS, the survival rate, weight, and neurological score of middle cerebral artery occlusion (MCAO) rats significantly improved. The hematoxylin and eosin (H&E) and TUNEL staining results showed that MM-Lip-Rg3/PNS can reduce neuronal apoptosis and inflammatory cell infiltration and protect the ischemic brain. In vivo biological experiments have shown that free Rg3, PNS, and MM-Lip-Rg3/PNS can alleviate inflammation and apoptosis, especially MM-Lip-Rg3/PNS, indicating that biomimetic liposomes can improve the therapeutic effects of drugs. Overall, MM-Lip-Rg3/PNS is a potential biomimetic nano targeted formulation for ischemic stroke therapy. [ABSTRACT FROM AUTHOR]

Effectively promoting employees' intrapreneurial behavior has become the focus of enterprises. This study takes the middle and grassroots employees in enterprises as subjects and explores the configuration effect of multiple influencing factors on employees' intrapreneurial behavior. Based on employee expectation theory and individual-environment matching theory, this study collates six influencing factors: entrepreneurial self-efficacy, entrepreneurial competence, task school level, perceived value, management support, and reward mechanism. A total of 163 samples were obtained, and the qualitative comparative analysis method based on fuzzy set was used to analyze the influence mechanism and result path of employees' intrapreneurial behavior from the perspective of the interaction between individual factors and organizational factors. Six influencing paths of employees' high intrapreneurial behavior were found, which can be divided into ability-driven and value-driven factors, revealing that the six factors can produce equivalent results in different configurations. Furthermore, five influencing paths of employees' non-high intrapreneurial behavior were divided into three types: ability obstacle type, perception obstacle type, and value obstacle type. These have an asymmetric causal relationship with employees' high intrapreneurial behavior. This study provides management support for effectively stimulating employees' intrapreneurial behavior. [ABSTRACT FROM AUTHOR]

Simple Summary: Bladder cancer (BC) is a common malignant tumor of the urinary system. Despite extensive advances in the treatment of BC, it remains one of the most recurring and life-threatening tumors. At present, there have been increasing reports of studies on the presence of aberrant regulation of RBPs in BC. However, the complex regulatory network of these RBPs in BC remains to be fully elaborated. RBPs have a very high potential in tumor prediction and personalized therapy. Moreover, only with a deep understanding of their regulatory mechanisms, expression characteristics, and potential binding sites, among other issues, will it become possible to apply RBPs to clinical applications. This article aims to summarize the research progress of RBPs in BC. It also attempts to encourage clinicians and researchers to devote attention this field of study and provides a reference for researchers who aspire to pursue a career in this area. RNA-binding proteins (RBPs) are key regulators of transcription and translation, with highly dynamic spatio-temporal regulation. They are usually involved in the regulation of RNA splicing, polyadenylation, and mRNA stability and mediate processes such as mRNA localization and translation, thereby affecting the RNA life cycle and causing the production of abnormal protein phenotypes that lead to tumorigenesis and development. Accumulating evidence supports that RBPs play critical roles in vital life processes, such as bladder cancer initiation, progression, metastasis, and drug resistance. Uncovering the regulatory mechanisms of RBPs in bladder cancer is aimed at addressing the occurrence and progression of bladder cancer and finding new therapies for cancer treatment. This article reviews the effects and mechanisms of several RBPs on bladder cancer and summarizes the different types of RBPs involved in the progression of bladder cancer and the potential molecular mechanisms by which they are regulated, with a view to providing information for basic and clinical researchers. [ABSTRACT FROM AUTHOR]

Tumor therapies have entered the immunotherapy era. Immune checkpoint inhibitors have achieved tremendous success, with some patients achieving long-term tumor control. Tumors, on the other hand, can still accomplish immune evasion, which is aided by immune checkpoints. The majority of immune checkpoints are membrane glycoproteins, and abnormal tumor glycosylation may alter how the immune system perceives tumors, affecting the body's anti-tumor immunity. Furthermore, RNA can also be glycosylated, and GlycoRNA is important to the immune system. Glycosylation has emerged as a new hallmark of tumors, with glycosylation being considered a potential therapeutic approach. The glycosylation modification of immune checkpoints and the most recent advances in glycosylation-targeted immunotherapy are discussed in this review. [ABSTRACT FROM AUTHOR]

Nowadays, the development of a metropolitan area has become one of the milestones for regions to move towards a high level of urbanization and has been elevated to a national strategy. In this paper, the economic and social development level of the four major metropolitan areas in the Yangtze River Delta region from 2005 to 2020 was dynamically evaluated by constructing an evaluation index system, which is based on a new development concept of China, and applying the "vertical and horizontal" evaluation method and model. Meanwhile, in order to reflect the core development concept of "leading small cities with large ones and coordinating development", this study incorporates the spatial and temporal economic connectivity between the central city and the surrounding small and medium sized member cities, as well as the industrial structure rationality and synergy into the index system, and applies the gravity model, the Thiel index and the industrial structure similarity coefficient to measure them, respectively. The study shows that the economic and social development of the four metropolitan areas is extremely uneven, with each area having certain shortcomings, and the development level of each area fluctuates significantly in terms of economic connectivity and industrial structure. Finally, relevant suggestions are put forward according to the shortcomings to provide reference for the future development direction of the metropolitan area, which is of great practical significance to promote the high-quality development of the regional economy. [ABSTRACT FROM AUTHOR]

The transformation of old and new technologies, the normalized crisis situation, and global economic integration blur industrial boundaries and cause the business pattern to fluctuate and become unsustainable, especially when considering the impact of the COVID-19 pandemic. This study focuses on crisis situations and combines the types of technology innovation (introduction, socialization, and differentiation) and sustainable business model innovation (efficiency, novelty, and co-benefit innovations) to theoretically analyze the dynamic impact of technology innovation on different types of sustainable business model innovations. Using a multi-case comparative analysis method, typical enterprises are selected as the sample cases. This study discusses the influences of different technology innovation schemes on sustainable business model innovation in different crisis situations. Enterprises should consider introducing technology for rapid value updates to maintain an efficient business model in an urgent production factor crisis, search for valuable and scarce technical components or introduce other entities to facilitate technical cooperation and form a novel business model in a market environment crisis, and use big data, artificial intelligence, and other technologies to create co-benefit business model innovation in a business ethics crisis. The conclusion guides enterprises and provides a framework for the optimal technical scheme under the corresponding crisis. [ABSTRACT FROM AUTHOR]

Local Convection in Dabie Mountains (LCDM) occurs more frequently over the Dabie Mountains and brings severe weather to adjacent areas. In order to understand the characteristics of LCDM, their spatial distribution, the monthly and diurnal variations, and possible mechanisms are investigated. Based on radar composite reflectivity data over the 5-y period of 2014–2018 during warm seasons (April–September), a total of 195 cases of LCDM are identified. The LCDM exhibits maximum frequency on the windward slopes of the Dabie Mountains with a secondary maximum on lee slopes. It is demonstrated that LCDM peaks in July and August, while their diurnal variation exhibits a major peak in the afternoon during 12:00–16:00 local solar time (LST). Most LCDM does not leave the Dabie Mountains (NoOut-Type), accounting for 89.7% overall, and has an average 3.5 h lifespan. In contrast, the lifespans of Out-Types (i.e., LCDMs that move away from the Dabie Mountains) are longer (5.8 h on average), while most Out-Type LCDMs develop on southern slopes ('South-Type') and a few are also reinforced on northern slopes ('North-Type'). The South-Type mainly produces short-duration heavy precipitation, while the 'North-Type' predominately generates thunderstorms high winds. It is suggested that LCDM is thermally induced, and that both the 'South-Type' and 'North-Type' are controlled by southerly wind perturbation. Lifting by upslope wind and heat sources over windward slopes has led to 'South-Type' development, while ascent induced by wave-like perturbations on lee slopes has led to 'North-Type'. These mechanisms should be further investigated in future work by using field experiments and numerical simulations. [ABSTRACT FROM AUTHOR]

Simple and effective methods for the detection of the level of blood glucose are closely linked to the monitoring of people’s health. In the study, MnO2 nanosheets with absorption range of 300 nm~500 nm and obvious yellow color were easily prepared and applied to detect glucose through their absorbance and color. The proposed method is based on the fact that a specific concentration of glucose can be quantitatively transformed into hydrogen peroxide (H2O2) under the catalytic effect of glucose oxidase. Based on the redox reaction of MnO2 with H2O2, yellow MnO2 can be converted into colorless Mn2+ to monitor the concentration of glucose. Under optimal conditions, a simple and effective visual assay for the sensitive and reliable detection of glucose was developed. The linear range was estimated to the range from 0 μM to 100 μM, with a detection limit of 12.8 μM. Furthermore, the proposed colorimetric assay based on MnO2 nanosheets can effectively detect blood glucose of clinical serum samples with accuracy and convenience. [ABSTRACT FROM AUTHOR]