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Simple Summary: Oral cancer is a challenging disease to treat, and new therapies are needed to improve patient outcomes. Okanin, a natural compound derived from Bidens pilosa L., has been known for its anti-inflammatory properties, but its effects on cancer, particularly oral cancer, are less understood. In this study, we investigated the anticancer potential of okanin in human oral cancer cells. Our results showed that okanin effectively reduced the growth of oral cancer cells by inducing cell death through mechanisms involving both apoptosis and pyroptosis. Additionally, okanin inhibited tumor growth in a mouse model of oral cancer. These findings suggest that okanin may be a promising natural compound for developing new treatments for oral cancer. Background: Okanin, a flavonoid compound derived from Bidens pilosa L., has garnered attention for its anti-inflammatory properties. Although Bidens pilosa is commonly used in healthcare products and functional foods, the anticancer potential of okanin, particularly in oral cancer, remains underexplored. This study aims to investigate the effects of okanin on oral cancer cell lines and its potential as a therapeutic agent. Methods: The study involved assessing the cytotoxic effects of okanin on oral cancer cell lines SAS, SCC25, HSC3, and OEC-M1. The IC50 values were determined using methylene blue assays, and the clonogenic capacity was evaluated through colony formation assays. Flow cytometry was used to analyze cell cycle progression and apoptosis. Caspase-3/7 activity assays and annexin V/7-AAD staining confirmed the induction of apoptosis and pyroptosis. In vivo efficacy was assessed using a SAS xenograft model, and immunohistochemical analysis of xenograft tissue was performed to examine pyroptosis-related markers. Results: Okanin exhibited potent cytotoxic effects with IC50 values of 12.0 ± 0.8, 58.9 ± 18.7, 18.1 ± 5.3, and 43.2 ± 6.2 μM in SAS, SCC25, HSC3, and OEC-M1 cells, respectively. It caused dose- and time-dependent reductions in cell viability and significantly impaired clonogenic capacity. Flow cytometry revealed G2/M cell cycle arrest and increased sub-G1 population, indicating cell cycle disruption and death. Okanin induced both apoptosis and pyroptosis, as confirmed by caspase-3/7 activity and annexin V/7-AAD staining. In vivo, okanin reduced tumor growth and involved pyroptosis-related markers such as CASP1, GSDMC, GSDMD, and GSDME. Conclusions: Okanin demonstrates significant anticancer potential, particularly in oral cancer, by inducing both apoptosis and pyroptosis. Its efficacy in reducing tumor growth in vivo further supports its potential as a novel therapeutic option. Further mechanistic studies are needed to elucidate the pathways involved in okanin-mediated cell death and to explore its clinical applications. [ABSTRACT FROM AUTHOR]

Introduction: Early identification of high-risk traumatic brain injury (TBI) patients is crucial for optimizing treatment strategies and improving outcomes. The C-reactive protein-to-lymphocyte ratio (CLR) reflects systemic immunology and inflammation function and serves as a new biomarker for patient stratification. This study aimed to assess the predictive value of the CLR for mortality in patients with isolated moderate to severe TBI. Methods: A retrospective analysis of trauma registry data from 2009 to 2022 was conducted, including 1641 adult patients with isolated moderate to severe TBI. Patient demographics, the CLR, injury characteristics, and outcomes were compared between deceased and surviving patients. Univariate and multivariate analyses were performed to identify mortality risk factors. The optimal CLR cut-off value for predicting mortality was determined using receiver operating characteristic (ROC) curve analysis. Results: The CLR was significantly higher in deceased patients compared to survivors (60.1 vs. 33.9, p < 0.001). The optimal CLR cut-off value for predicting mortality was 54.5, with a sensitivity of 0.328 and a specificity of 0.812. The area under the ROC curve was 0.566, indicating poor discriminative ability. In the multivariate analysis, the CLR was not a significant independent predictor of mortality (OR 1.03, p = 0.051). After propensity score matching to attenuate the difference in baseline characteristics, including sex, age, comorbidities, conscious level, and injury severity, the high-CLR group (CLR ≥ 54.5) did not have significantly higher mortality compared to the low-CLR group (CLR < 54.5). Conclusion: While the CLR was associated with mortality in TBI patients, it demonstrated poor discriminative ability as a standalone predictor. The association between a high CLR and worse outcomes may be primarily due to other baseline patient and injury characteristics, rather than the CLR itself. [ABSTRACT FROM AUTHOR]

In recent years, research on attribute-based encryption (ABE) has expanded into the quantum domain. Because a traditional single authority can cause the potential single point of failure, an improved lattice-based quantum-resistant identity authentication and policy attribute encryption scheme is proposed, in which the generation of random values is optimized by adjusting parameters in the Gaussian sampling algorithm to improve overall performance. Additionally, in the key generation phase, attributes are processed according to their shared nature, which reduces the computational overhead of the authorization authority. In the decryption phase, the basis transformation of the Lenstra–Lenstra–Lovász (LLL) lattice reduction algorithm is utilized to rapidly convert shared matrices into the shortest vector form, which can reduce the computational cost of linear space checks. The experimental results demonstrate that the proposed method not only improves efficiency but also enhances security compared with related schemes. [ABSTRACT FROM AUTHOR]

This paper delved into the thermal dynamics and stability of Wireless Power Transfer (WPT) systems, with a focus on the temperature effects on the coil structure. Using the Finite Element Method (FEM), this study investigated both unidirectional and bidirectional coupling field simulations, assessing their impacts on the transmission efficiency of LCL-resonant WPT systems. The boundary conditions and processes of the electromagnetic–thermal coupling field related to coil loss were analyzed, as well as the dynamic thermal balance in the bidirectional coupling field model. It was found that there is a significant temperature variation across the coil, with the highest temperatures at the central position and the lowest at the edges. This temperature rise notably changed the electrical parameters of the system, leading to variations in its operating state and a reduction in transmission efficiency. A constant coil voltage control strategy was more effective in mitigating the temperature rise compared to a constant coil current strategy, providing valuable insight for enhancing the efficiency and stability of WPT systems. [ABSTRACT FROM AUTHOR]

In general, the fuel cell electric vehicle (FCEV) is regarded as more environmentally friendly than other vehicles. However, the commercialization of FCEV technology is hardly fulfilled due to high-cost fuel cells and an inadequate refueling infrastructure. Different technological trajectories of fuel cells are fiercely competitive, and related technologies are iterating quickly. It is an open issue in terms of what are the technological advances achieved or the opportunities for innovators. The paper proposes a novel approach to identify the key components of an FCEV by constructing the directed co-occurrence network of the International Patent Classification (IPC) and then adopts the Natural Language Processing (NLP) to construct the matrix of technology characteristics and functions. It is suitable to analyze the sentence structure of Subject–Action–Object (SAO) in patent documents by utilizing the NLP technology, which can help computers understand the text and communicate with us. The paper finds that the advances achieved in the fuel cell field are fuel cell composition, manufacturing fuel cells, and providing energy using fuel cells, and the advance in electric motors is supplying power for fuel cell vehicles, while the advances in hydrogen storage are to manage and store hydrogen. By contrast, the opportunities for innovators are to develop the control, diagnosis, and performance of the control system and hydrogen filling. This paper will be a contribution towards a better understanding of the advances and opportunities for developing FCEV technology. [ABSTRACT FROM AUTHOR]

As a critical material for high-temperature components of aero-engines, the mechanical properties of Ti65 alloy, subjected to high-temperature and long-term thermal exposure, directly affect its service safety. The room-temperature tensile properties of the Ti65 alloy after thermal exposure to temperatures ranging from 450 °C to 650 °C for 100 h were investigated. The results indicate that as the thermal exposure temperature increases, the strength of Ti65 alloy initially increases and then decreases, while ductility exhibits a decreasing trend. The strength of the thermally exposed alloy positively correlates with the size and content of the α2 phase. The ductility of the thermally exposed alloy is comprehensively influenced by the surface oxidation behavior, α2 phase, and silicides. After the prolonged thermal exposure, stress concentration at the crack tips within the oxide layer was enhanced with the increased thickness of the surface TiO2 oxide layer, leading to premature fracture due to reduced alloy ductility. Furthermore, the α2 phase in the matrix promotes the planar slip of dislocations, while silicides at the α/β phase boundaries hinder dislocation motion, causing dislocation pile-ups. Both behaviors facilitate crack nucleation and deteriorate alloy ductility. [ABSTRACT FROM AUTHOR]

Simple Summary: Parasitoid wasps play a crucial role in controlling sanitary pests such as cockroaches, which pose a threat to human health. Tetrastichus hagenowii is an effective parasitoid wasp for the biological control of cockroaches, particularly the American cockroach (Periplaneta americana). This study aimed to evaluate the stability of potential reference genes in T. hagenowii. Seven candidate reference genes were rigorously selected and their reliability was assessed using RT-qPCR. Stability analysis across different developmental stages of T. hagenowii was conducted using five algorithmic methods, ultimately identifying α-tubulin as the most stable reference gene. Our research not only provides reliable reference genes to enhance studies on gene expression and functional genomics related to T. hagenowii but also offers valuable insights for the regulation of parasitoid wasps in cockroach control strategies. Parasitoid wasps play a crucial role in the efficient control of pests, a substantial menace to human health and well-being. Tetrastichus hagenowii (Ratzeburg) stands out as the most effective egg parasitoid wasp for controlling American cockroaches, but accurate and stable reference genes for quantitative real-time polymerase chain reaction of T. hagenowii genes are still lacking. In this study, we assessed seven candidate nuclear genes, including α-tubulin (α-TUB), elongation factor-1-alpha (EF-1α), β-actin (Actin), ribosomal protein 49 (RP49), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), nicotinamide adenine dinucleotide (NADH), and elongation factor 2 (EF2) of T. hagenowii. By analyzing expression stability with four algorithms (Delta Ct, geNorm, NormFinder, and BestKeeper), as well as comprehensive ranking with RefFinder, we identified α-TUB as the most stable reference gene for the larval, pupal, female adult, and male adult stages. Subsequently, we estimated the transcript levels of vitellogenin (Vg) and cuticle protein (CP) after normalization with α-TUB across various developmental stages. Significantly higher expression levels of CP and Vg were observed in pupae and female adults, respectively, consistent with previous findings in other insects. This study offers a reliable reference gene for normalizing transcription levels of T. hagenowii genes. [ABSTRACT FROM AUTHOR]

Traditional drug development is a long and expensive process with high rates of failure. This has prompted the pharmaceutical industry to seek more efficient drug development frameworks, driving the emergence of organ-on-a-chip (OOC) based on microfluidic technologies. Unlike traditional animal experiments, OOC systems provide a more accurate simulation of human organ microenvironments and physiological responses, therefore offering a cost-effective and efficient platform for biomedical research, particularly in the development of new medicines. Additionally, OOC systems enable quick and real-time analysis, high-throughput experimentation, and automation. These advantages have shown significant promise in enhancing the drug development process. The success of an OOC system hinges on the integration of specific designs, manufacturing techniques, and biosensors to meet the need for integrated multiparameter datasets. This review focuses on the manufacturing, design, sensing systems, and applications of OOC systems, highlighting their design and sensing capabilities, as well as the technical challenges they currently face. [ABSTRACT FROM AUTHOR]

Geopolymers assume an irreplaceable position in the engineering field on account of their numerous merits, such as durability and high temperature resistance. Nevertheless, geopolymers also demonstrate brittleness. In this study, geotextiles with different layers were added to geopolymer to study its compressive strength and stability. Laboratory materials such as alkali activators, geotextiles and granite residual soil (GRS) were utilized. The samples were characterized via XRD, TG-DTG, SEM-EDS and FT-IR. The results indicate that the toughness of geopolymer is significantly enhanced by adding geotextiles, and the strength increase is most obvious when adding one layer of geotextile: the strength increased from 2.57 Mpa to 3.26 Mpa on the 14th day, an increase of 27%. Additionally, the D-W cycle has a great influence on geotextile polymers. On the 14th day, the average strength of the D-W cyclic sample (1.935 Mpa) was 1.305 Mpa smaller than that of the naturally cured sample (3.24 Mpa), and the strength decreased by 40%. These discoveries offer a novel approach for further promoting the application of geopolymers, especially in the field of foundation reinforcement. [ABSTRACT FROM AUTHOR]

The assessment of catechin content stands as a pivotal determinant of tea quality. In tea production and quality grading, the development of accurate and non-destructive techniques for the accurate prediction of various catechin content is paramount. Near-infrared spectroscopy (NIRS) has emerged as a widely employed tool for analyzing the chemical composition of tea. Nevertheless, the spectral information obtained from NIRS faces challenges when discerning different types of catechins in black tea, owing to their similar physical and chemical properties. Moreover, the vast number of NIRS wavelengths exceeds the available tea samples, further complicating the accurate assessment of catechin content. This study introduces a novel deep learning approach that integrates specific wavelength selection and attention mechanisms to accurately predict the content of various catechins in black tea simultaneously. First, a wavelength selection algorithm is proposed based on feature interval combination sensitivity segmentation, which effectively extracts the NIRS feature information of tea. Subsequently, a one-dimensional convolutional neural network (CNN) incorporating channel and spatial–sequential attention mechanisms is devised to independently extract the key features from the selected wavelength variables. Finally, a multi-output predictor is employed to accurately predict the four main catechins in tea. The experimental results demonstrate the superiority of the proposed model over existing methods in terms of prediction accuracy and stability (R2 = 0.92, RMSE = 0.018 for epicatechin; R2 = 0.96, RMSE = 0.11 for epicatechin gallate; R2 = 0.97, RMSE = 0.14 for epigallocatechin; R2 = 0.97, RMSE = 0.32 for epigallocatechin gallate). This innovative deep learning approach amalgamates wavelength selection with attention mechanisms, provides a new perspective for the simultaneous assessment of the major components in tea, and contributes to the advancement of precision management in the tea industry's production and grading processes. [ABSTRACT FROM AUTHOR]

To meet the requirements of the crab growth environment regarding aquatic plant density and improve the efficiency of aquatic plant clearing, this paper shows the development process of a fully automatic aquatic plant combing machine for crab farming. It proposed the use of torsion spring hooks to replace traditional cutting blades to break tangled aquatic plants, reducing the length of aquatic plants in dense areas and thus controlling the density of aquatic plants in crab ponds. Through theoretical analysis and calculation of the torsion spring hooks, it was ensured that they could meet the design requirements, and transient dynamic simulation tests were conducted based on ANSYS. Finally, experimental verification was carried out. The performance test results of the torsion spring hooks showed that the torsion force generated within a certain torsion angle range could break the aquatic plant, and obstacles could be avoided through self-deformation. The water performance test results showed that the average clearing efficiency of the whole machine for aquatic plants was 4.92 mu/h, the missed clearing rate of aquatic plants was 0.44%, and the crab injury rate was 0.028%. The design of this machine can provide a reference for the development of aquatic plant harvesters for crab farming. [ABSTRACT FROM AUTHOR]

The propagation of antibiotic resistance in environments, particularly aquatic environments that serve as primary pathways for antibiotic resistance genes (ARGs), poses significant health risks. The impact of nutrients, as key determinants of bacterial growth and metabolism, on the propagation of ARGs, particularly extracellular ARGs (eARGs), remains poorly understood. In this study, we collected microorganisms from the Yangtze River and established a series of microcosms to investigate how variations in nutrient levels and delivery frequency affect the relative abundance of intracellular ARGs (iARGs) and eARGs in bacterial communities. Our results show that the relative abundance of 7 out of 11 representative eARGs in water exceeds that of iARGs, while 8 iARGs dominate in biofilms. Notably, iARGs and eARGs consistently exhibited opposite responses to nutrient variation. When nutrient levels increased, iARGs in the water also increased, with the polluted group (COD = 333.3 mg/L, COD:N:P = 100:3:0.6, m/m) and the eutrophic group (COD = 100 mg/L, COD:N:P = 100:25:5, m/m) showing 1.2 and 3.2 times higher levels than the normal group (COD = 100 mg/L, COD:N:P = 100:10:2, m/m), respectively. In contrast, eARGs decreased by 6.7% and 8.4% in these groups. On the other hand, in biofilms, higher nutrient levels led to an increase in eARGs by 1.5 and 1.7 times, while iARGs decreased by 17.5% and 50.1% in the polluted and eutrophic groups compared to the normal group. Moreover, while increasing the frequency of nutrient delivery (from 1 time/10 d to 20 times/10 d) generally did not favor iARGs in either water or biofilm, it selectively enhanced eARGs in both. To further understand these dynamics, we developed an ARGs-nutrient model by integrating the Lotka–Volterra and Monod equations. The results highlight the complex interplay of bacterial growth, nutrient availability, and mechanisms such as horizontal gene transfer and secretion influencing ARGs' propagation, driving the opposite trend between these two forms of ARGs. This contrasting response between iARGs and eARGs contributes to a dynamic balance that stabilizes bacterial resistance levels amid nutrient fluctuations. This study offers helpful implications regarding the persistence of bacterial resistance in the environment. [ABSTRACT FROM AUTHOR]

The annual co-circulation of two influenza A subtypes, H1N1 and H3N2, viruses in humans poses significant public health threats worldwide. However, the continuous antigenic drift and shift of influenza viruses limited the effectiveness of current seasonal influenza vaccines, necessitating the development of new vaccines against both seasonal and pandemic viruses. One potential solution to this challenge is to improve inactivated vaccines by including multiple T-cell epitopes. In this study, we designed stabilized trimeric recombinant mosaic HA proteins named HAm, which contain the most potential HA T-cell epitopes of seasonal influenza A virus. We further evaluated the antigenicity, hemagglutinin activity, and structural integrity of HAm and compared its immunogenicity and efficacy to a commercial quadrivalent inactivated influenza vaccine (QIV) in mice. Our results demonstrated that the HAm vaccine was able to induce broadly cross-reactive antibodies and T-cell responses against homologous, heterologous, and heterosubtypic influenza-naive mice. Additionally, the HAm antigens outperformed QIV vaccine antigens by eliciting protective antibodies against panels of antigenically drifted influenza vaccine strains from 2009 to 2024 and protecting against ancestral viruses' lethal challenge. These results suggest that the HAm vaccine is a promising potential candidate for future universal seasonal and pandemic influenza vaccine development. [ABSTRACT FROM AUTHOR]

Introduction: Stress-induced hyperglycemia (SIH) and malnutrition are common in trauma patients and are linked to worse outcomes. This study examined the influence of nutritional status, determined by the Geriatric Nutritional Risk Index (GNRI), on the incidence of SIH in trauma patients. Methods: A retrospective analysis was conducted on adult trauma patients admitted to a Level I trauma center from 1 January 2009 to December 31, 2021. Patients were categorized into four groups: SIH, diabetic hyperglycemia (DH), diabetic normoglycemia (DN), and non-diabetic normoglycemia (NDN). Nutritional status was assessed using GNRI: high risk (GNRI < 82), moderate risk (82 ≤ GNRI < 92), low risk (92 ≤ GNRI ≤ 98), and no risk (GNRI > 98). Incidence of SIH and outcomes were analyzed across GNRI groups. Results: SIH was associated with higher mortality across all GNRI groups compared to NDN, with the highest rate (45.7%) in the high-risk group. Mortality decreased as GNRI increased in all glucose groups. NDN patients had the lowest mortality rates across GNRI groups. There was no correlation between GNRI and SIH incidence (p = 0.259). Conclusion: SIH significantly influenced mortality across all nutritional status groups, with the highest impact in malnourished patients. Although malnutrition did not affect SIH incidence, both SIH and poor nutritional status independently contributed to worse trauma outcomes. Targeted management of hyperglycemia and nutritional deficiencies is crucial for improving survival. [ABSTRACT FROM AUTHOR]

The TATA-box binding protein (TBP) and TBP-associated factors (TAFs) constitute the transcription factor IID (TFIID), a crucial component of RNA polymerase II, essential for transcription initiation and regulation. Several TFIID subunits are shared with the Spt–Ada–Gcn5–acetyltransferase (SAGA) coactivator complex. Recent research has revealed the roles of TBP and TAFs in organogenesis and stress adaptation. In this study, we identified 1 TBP and 21 putative TAFs in the mungbean genome, among which VrTAF5, VrTAF6, VrTAF8, VrTAF9, VrTAF14, and VrTAF15 have paralogous genes. Their potential involvement in abiotic stress responses was also investigated here, including high salinity, water deficit, heat, and cold. The findings indicated that distinct genes exerted predominant influences in the response to different abiotic stresses through potentially unique mechanisms. Specifically, under salt stress, VrTBP, VrTAF2, and VrTAF15–1 were strongly induced, while VrTAF10, VrTAF11, and VrTAF13 acted as negative regulators. In the case of water-deficit stress, it was likely that VrTAF1, VrTAF2, VrTAF5–2, VrTAF9, and VrTAF15–1 were primarily involved. Additionally, in response to changes in ambient temperature, it was possible that genes such as VrTAF5–1, VrTAF6–1, VrTAF9–2, VrTAF10, VrTAF13, VrTAF14b–2, and VrTAF15–1 might play a dominant role. This comprehensive exploration of VrTBP and VrTAFs can offer a new perspective on understanding plant stress responses and provide valuable insights into breeding improvement. [ABSTRACT FROM AUTHOR]

Plants encounter numerous adversities during growth, necessitating the identification of common stress activators to bolster their resistance. However, the current understanding of these activators' mechanisms remains limited. This study identified three anti-stress activators applicable to apple trees, all of which elevate plant proline content to enhance resistance against various adversities. The results showed that the application of these sugar substitutes increased apple proline content by two to three times compared to the untreated group. Even at a lower concentration, these activators triggered plant stress resistance without compromising apple fruit quality. Therefore, these three sugar substitutes can be exogenously sprayed on apple trees to augment proline content and fortify stress resistance. Given their effectiveness and low production cost, these activators possess significant application value. Since they have been widely used in the food industry, they hold potential for broader application in plants, fostering apple industry development. [ABSTRACT FROM AUTHOR]

Hybrid energy supply systems are widely utilized in modern manufacturing processes, where accurately predicting energy consumption is essential not only for managing productivity but also for driving sustainable development. Effective energy management is a cornerstone of sustainable manufacturing, reducing waste and enhancing efficiency. However, conventional studies often focus solely on predicting single types of energy consumption and overlook the integration of physical laws and information, which are essential for a comprehensive understanding of energy dynamics. In this context, this paper introduces a multi-task physics-informed multi-gate mixture-of-experts (pi-MMoE) model that not only considers multiple forms of energy consumption but also incorporates physical principles through the integration of physical information and multi-task modeling. Specifically, a detailed analysis of manufacturing processes and energy patterns is first conducted to study various energy types and extract relevant physical laws. Next, using industry insights and thermodynamic principles, key equations for energy balance and conversion are derived to create a physics-based loss function for model training. Finally, the pi-MMoE model framework is constructed, featuring multi-expert networks and gating mechanisms to balance cross-task knowledge sharing and expert learning. In a case study of a textile factory, the pi-MMoE model reduced electricity and steam prediction errors by 14.28% and 27.27%, respectively, outperforming traditional deep learning methods. This demonstrates that the model can improve prediction performance, providing a novel approach to intelligent energy management and promoting sustainable development in manufacturing. [ABSTRACT FROM AUTHOR]

Background: Hypersensitivity to the new dermal injectable porcine-based collagen with lidocaine featuring a novel cross-linking technology (test filler) for nasolabial fold correction was compared to the commercially available traditional cross-linked dermal injectable porcine-based collagen with lidocaine (control filler). Methods: Recruited participants (n = 279) received a single 0.1 mL intradermal injection of either test filler or control filler in the left forearm as a screening skin allergy test. Injection sites were assessed clinically at 24 h post-implant. Treatment was given to 252 successfully screened participants, and injection sites were monitored for 21 days. Immunological examinations were performed at screening and then at 4 and 24 weeks post-treatment. Observations for adverse events continued until the 52nd week. Results: Intradermal allergy testing results were negative for all the test recipients (0/124) and positive for two control recipients (2/132, 1.5%). Most of the participants exhibited no changes in serum immunoglobulin (IgG, IgM) and complement (C3, C4) levels. No serious adverse events related to the device were recorded. Most adverse events were common complications of dermal filler treatment and were related to the injection site. Most adverse effects were resolved or under control by 52 weeks. Conclusions: Hypersensitivity reactions with the test filler were lower than those with the control filler, validating the safe use of test filler for nasolabial fold correction without the need for pretreatment skin testing. [ABSTRACT FROM AUTHOR]

Sound Event Detection and Localization (SELD) is a comprehensive task that aims to solve the subtasks of Sound Event Detection (SED) and Sound Source Localization (SSL) simultaneously. The task of SELD lies in the need to solve both sound recognition and spatial localization problems, and different categories of sound events may overlap in time and space, making it more difficult for the model to distinguish between different events occurring at the same time and to locate the sound source. In this study, the Dual-conv Coordinate Attention Module (DCAM) combines dual convolutional blocks and Coordinate Attention, and based on this, the network architecture based on the two-stage strategy is improved to form the SELD-oriented Two-Stage Dual-conv Coordinate Attention Model (TDCAM) for SELD. TDCAM draws on the concepts of Visual Geometry Group (VGG) networks and Coordinate Attention to effectively capture critical local information by focusing on the coordinate space information of the feature map and dealing with the relationship between the feature map channels to enhance the feature selection capability of the model. To address the limitation of a single-layer Bi-directional Gated Recurrent Unit (Bi-GRU) in the two-stage network in terms of timing processing, we add to the structure of the two-layer Bi-GRU and introduce the data enhancement techniques of the frequency mask and time mask to improve the modeling and generalization ability of the model for timing features. Through experimental validation on the TAU Spatial Sound Events 2019 development dataset, our approach significantly improves the performance of SELD compared to the two-stage network baseline model. Furthermore, the effectiveness of DCAM and the two-layer Bi-GRU structure is confirmed by performing ablation experiments. [ABSTRACT FROM AUTHOR]

The insulation of high-voltage direct-current (HVDC) cables experiences a short period of voltage polarity reversal when the power flow is adjusted, leading to sever field distortion in this situation. Consequently, improving the insulation performance of the composite insulation structure in these cables has become an urgent challenge. In this paper, SiC-SR (silicone rubber) and TiO2-SR nanocomposites were chosen for fabricating HVDC cable accessories. These nanocomposites were prepared using the solution blending method, and an electro-acoustic pulse (PEA) space charge test platform was established to explore the electron transfer mechanism. The space charge characteristics and field strength distribution of a double-layer dielectric composed of cross-linked polyethylene (XLPE) and nano-composite SR at different concentrations were studied during voltage polarity reversal. Additionally, a self-built breakdown platform for flake samples was established to explore the effect of the nanoparticle doping concentration on the breakdown field strength of double-layer composite media under polarity reversal. Therefore, a correlation was established between the micro electron transfer process and the macro electrical properties of polymers (XLPE/SR). The results show that optimal concentrations of nano-SiC and TiO2 particles introduce deep traps in the SR matrix, significantly inhibiting charge accumulation and electric field distortion at the interface, thereby effectively improving the dielectric strength of the double-layer polymers (XLPE/SR). [ABSTRACT FROM AUTHOR]

Background: Traumatic brain injury (TBI) is a major cause of mortality and disability worldwide, with severe cases significantly increasing the risk of complications and long-term mortality. The Geriatric Trauma Outcome Score (GTOS), based on age, injury severity, and transfusion need, has been validated for predicting mortality in older trauma patients, but its utility in predicting mortality for TBI patients remains unexplored. Methods: This retrospective study included 5543 adult trauma patients with isolated moderate to severe TBI, defined by head Abbreviated Injury Scale (AIS) scores of ≥ 3, from 1998 to 2021. GTOS was calculated with the following formula: age + (Injury Severity Score × 2.5) + 22 (if transfused within 24 h). The area under the receiver operating characteristic curve (AUROC) assessed GTOS's ability to predict mortality. The optimal GTOS cutoff value was determined using Youden's index. Mortality rates were compared between high- and low-GTOS groups, separated by the optimal GTOS cutoff value, including a propensity score-matched analysis adjusting for baseline characteristics. Results: Among 5543 patients, mortality was 8.3% (462 deaths). Higher mortality is correlated with male sex, older age, higher GTOS, and comorbidities like hypertension, coronary artery disease, and end-stage renal disease. The optimal GTOS cut-off for mortality prediction was 121.5 (AUC = 0.813). Even when the study population was matched by propensity score, patients with GTOS ≥121.5 had much higher odds of death (odds ratio 2.64, 95% confidence interval 1.93–3.61, p < 0.001) and longer hospital stays (mean 16.7 vs. 12.2 days, p < 0.001) than those with GTOS < 121.5. Conclusions: These findings support the idea that GTOS is a useful tool for risk stratification of in-hospital mortality in isolated moderate to severe TBI patients. However, we encourage further research to refine GTOS for better applicability in TBI patients. [ABSTRACT FROM AUTHOR]

This paper proposes a novel yet simple approach to the adaptive finite element (FE) analysis of the first-order Initial Value Problems (IVPs) in the maximum norm by introducing the reduced element technique. In the present approach, the FE solution uh of the conventional Galerkin element of degree m + 1 is decomposed into two parts: a reduced solution u R h from the reduced element of degree m obtained by ignoring the highest degree term of uh, and a built-in point-wise error estimator ε R h directly given by the ignored term. Since the end node solutions of the reduced element are inherited from the full order element, it gains O(h2m+2) accuracy and achieves a nodal/element accuracy ratio as high as two, which greatly enhances its adaptive capability regarding solving IVPs on long time domains. The related error analysis is addressed and a complete adaptivity algorithm is given. Typical numerical examples of both linear and nonlinear IVPs of both single and systems of equations are presented to show the validity and effectiveness of the proposed approach. [ABSTRACT FROM AUTHOR]

Due to the deficiency of radio spectrum resources caused by the progress in technology, cognitive radio networks (CRNs) have made significant progress. CRNs have two types of users, namely, primary users (PUs) and secondary users (SUs). Considering that PUs have a higher priority and diversified data transmission requirements, this study divides PUs into two levels, namely, PU1s with a higher priority and PU2s with a lower priority. On the other hand, the occurrence of failures is inevitable in CRNs, which affects the data transmission of users. In this paper, combined with an adjustable PU packets transmission rate mechanism, a communication failure and repair mechanism with classified PUs based on the single-channel CRNs is proposed, and different preemption principles are set according to different system states. A queueing model is established and analyzed with a Markov chain, the performance index expressions that need targeted research are listed, numerical experiments are conducted, and the system performance change trends are obtained. The comparison experiment shows that the proposed communication failure and repair mechanism with classified PUs can improve the throughput of PU1 packets and reduce the blocking rate of PU1 packets compared with the conventional communication failure and repair mechanisms with unclassified PUs. [ABSTRACT FROM AUTHOR]

Following hydraulic fracturing operations within shale reservoirs, there frequently exists a considerable volume of residual oil that remains encapsulated within the matrix, thereby impeding the singular shale well's productivity from attaining projected yields. In pursuit of augmenting the recovery efficiency of shale oil, the industry has widely adopted a post-fracture shut-in strategy within shale oil wells. This methodology is predicated on the aspiration to escalate both the production output and the recovery factor of the oil well by leveraging the imbibition and displacement mechanisms of the fracturing fluid throughout the shut-in interval. There are many kinds of surfactants, and how to select surfactants suitable for shale reservoirs from these many surfactants has become a key issue in improving shale reservoir recovery. In this study, a new surfactant screening method for improving imbibition recovery in shale reservoirs is proposed. An interfacial tension test, contact angle test, and anti-adsorption test are carried out for the collected surfactant products, and the interfacial tension, contact angle, and anti-adsorption are gradually used as indicators. The type of surfactant is initially screened. On this basis, the static imbibition experiment of shale is made to determine the type and concentration of surfactants suitable for shale oil development. The results show that the surfactants screened by this method have the characteristics of decreasing oil–water interfacial tension, varying rock wettability, and strong anti-adsorption, which can effectively improve imbibition efficiency. The study results herein can provide technical support for optimizing shale oil surfactants and provide a new idea for improving oil exploitation in low-permeability reservoirs. [ABSTRACT FROM AUTHOR]

Weird horizontal shapes of branches, in large branching estuaries, often cause significant flood risks and environment-related problems. People usually resort to engineering methods to improve the horizontal shape of the weird branches and solve related issues. The responses of the riverbed evolution of a branching estuary to anthropogenic activity are complicated because of complex estuarine hydrodynamics and sediment transports, especially when the project locates specially (e.g., at estuary outlets). The North Branch of the Yangtze Estuary has a narrow upper reach which is almost orthogonal to the South Branch and has a trumpet-shaped lower reach with a wide outlet. The weird horizontal shape of the North Branch brings significant flood risks to cities along this branch, the shrinkage of its entrance, and other problems. In this study, a regulation of the North Branch, which is launched at Guyuan Sand (GYS) just outside the exit of the North Branch, is taken as an example. The GYS regulation aims to improve the weird horizontal shape of the North Branch by building new layouts of outlets, by which people decrease the flood risk of the surrounding cities. The GYS regulation is studied using a 2D numerical model. The riverbed evolution of the Yangtze Estuary in a typical hydrological year is simulated, while the water/sediment fluxes at cross-sections of branches in the estuary during a spring/neap tide are quantitatively calculated. It is found that the regulation changes the rotational flows near the shore, and further reshapes the estuarine circulations of mass inside the outlets, especially exchanges of water/sediment between different branches. The regulation directly changes the riverbed evolution at the outlet of the North Branch, and meanwhile has significant indirect influences on the riverbed evolution of the entrance of the North Branch. The varying riverbed evolution at the entrance of the North Branch and the varying water/sediment fluxes, under different designs of regulations, are related and analyzed. An essential improvement for the weird horizontal shape of the North Branch by an engineering method is shown to be possible, while the regulation mechanism of the engineering method and the response of estuarine riverbed evolution to the regulation are clarified. This study provides a new insight for improving estuarine branches with weird horizontal shapes, by reshaping the tidal processes and the accompanying sediment transports in a branching estuary. [ABSTRACT FROM AUTHOR]

This article addresses the challenge of maintaining water quality in urban landscape lakes in water-scarce cities using transparency as the key indicator. The sensitivity of water transparency to nine water quality parameters, including chlorophyll a and inorganic suspended solids, in 16 urban landscape lakes of the city of Taiyuan was evaluated using the Sobol and Morris sensitivity analysis methods. The results indicate that for water bodies supplied by surface water, critical factors include chlorophyll a and hydraulic retention time. For water bodies supplied by tap water, inorganic suspended solids and total phosphorus are more significant. Water bodies with a dual function of urban flood control should focus on dissolved oxygen, ammonium nitrogen, and chemical oxygen demand. Based on these findings, targeted management strategies are proposed to enhance algae management, control suspended solids input, and adjust water retention times, aiming to improve the transparency and quality of Taiyuan's urban landscape. [ABSTRACT FROM AUTHOR]

Understanding the genetic basis of salt resistance in crops is crucial for agricultural productivity. This study investigates the phenotypic and genetic basis of salt stress response in rice (Oryza sativa L.), focusing on germination and seedling traits. Under salt stress conditions, significant differences were observed in seed germination and seedling traits between parental LH99 (Indica rice LuHui 99) and SN265 (japonica rice ShenNong 265). Transgressive segregation was evident within the RIL population, indicating complex genetic interactions. Nine QTLs were detected at germination and seedling stages under salt stress, namely qSGE5 and qSGE7 for seed germination energy (SGE); qSGP7 for seed germination percentage (SGP); qSSH7, qSSH9-1, and qSSH9-2 for seeding height (SSH); qSRN6 for root number (SRN); and qSDW6 and qSDW9 for dry weight (SDW). Among them, qSSH9-1 and qSDW9 were localized in the same interval, derived from the salt-resistant parent SN265. PCA revealed distinct trait patterns under salt stress, captured by six PCs explaining 81.12% of the total variance. PC composite scores were used to localize a QTL associated with early salt resistance in rice qESC9, which was located in the same interval as qSSH9-1 and qSDW9, and was subsequently unified under the name qESC9, an important QTL for early-growth salt tolerance in rice. Correlation analysis also confirmed a relationship between alleles of qESC9 and the resistance to salt, underscoring the critical role this locus plays in the determination of overall salt tolerance in rice. Physiological analyses of extreme phenotype lines highlighted the importance of ion exclusion mechanisms in salt-resistant lines, while salt-susceptible lines exhibited elevated oxidative stress and impaired antioxidant defense, contributing to cellular damage. This comprehensive analysis sheds light on the genetic and physiological mechanisms underlying salt stress response in rice, providing valuable insights for breeding programs aimed at enhancing salt resistance in rice. [ABSTRACT FROM AUTHOR]

Simple Summary: Head and neck cancer disparities in outcomes for Black Americans have been well recognized. However, the specific drivers of the inferior outcomes remain poorly understood. We investigated the biologic features of patient oral cancers and performed a follow-up study of the patient post-surgery recurrences and metastases aiming to explore potential mechanisms that might underpin the poorer outcomes among Black American patients. It was found that high levels of tumor stemness and tumor-promoting stromal characteristics were linked to patient recurrence and metastasis. There were more cases of Black American than White American exhibiting high stemness traits and strong tumor-promoting stromal features associated with tumor recurrences and metastases, although the investigated cases displayed comparable clinical diagnoses. Our findings revealed that the differences in tumor stemness and stromal property among cancers with similar diagnoses contribute to patient outcome disparities. Black Americans (BAs) with head and neck cancer (HNC) have worse survival outcomes compared to the White patients. While HNC disparities in patient outcomes for BAs have been well recognized, the specific drivers of the inferior outcomes remain poorly understood. Here, we investigated the biologic features of patient tumor specimens obtained during the surgical treatment of oral cancers and performed a follow-up study of the patients' post-surgery recurrences and metastases with the aim to explore whether tumor biologic features could be associated with the poorer outcomes among BA patients compared with White American (WA) patients. We examined the tumor stemness traits and stromal properties as well as the post-surgery recurrence and metastasis of oral cancers among BA and WA patients. It was found that high levels of tumor self-renewal, invasion, tumorigenesis, metastasis, and tumor-promoting stromal characteristics were linked to post-surgery recurrence and metastasis. There were more BA than WA patients demonstrating high stemness traits and strong tumor-promoting stromal features in association with post-surgery tumor recurrences and metastases, although the investigated cases displayed clinically comparable TNM stages and histological grades. These findings demonstrated that the differences in tumor stemness and stromal property among cancers with comparable clinical diagnoses contribute to the outcome disparity in HNCs. More research is needed to understand the genetic and molecular basis of the biologic characteristics underlying the inferior outcomes among BA patients, so that targeting strategies can be developed to reduce HNC disparity. [ABSTRACT FROM AUTHOR]

Sorghum is an important C4 crop with various food and nonfood uses. Although improvements through hybridization and selection have been exploited, the introduction of genetic variation and the development of new genotypes in sorghum are still limited. Fast-neutron (FN) mutagenesis is a very effective method for gene functional studies and to create genetic variability. However, the full spectrum of FN-induced mutations in sorghum is poorly understood. To address this, we generated an FN-induced mutant population from the inbred line 'BTx623' and sequenced 40 M1 seedlings to evaluate the mutagenic effects of FNs on sorghum. The results show that each line had an average of 43.7 single-base substitutions (SBSs), 3.7 InDels and 35.15 structural variations (SVs). SBSs accounted for approximately 90.0% of the total number of small mutations. Among the eight treatment groups, FN irradiation at a dose of 19 Gy generated the highest number of mutations. The ratio of transition/transversion ranged from 1.77 to 2.21, and the G/C to A/T transition was the most common substitution in all mutant lines. The distributions of the identified SBSs and InDels were similar and uneven across the genome. An average of 3.63 genes were mutated in each mutant line, indicating that FN irradiation resulted in a suitable density of mutated genes, which can be advantageous for improving elite material for one specific or a few traits. These results provide a basis for the selection of the suitable dose of mutagen and new genetic resources for sorghum breeding. [ABSTRACT FROM AUTHOR]

Composite materials are widely used in the new generation of aviation equipment due to their comprehensive performance. However, the part fixture is usually difficult during the machining of composites with soft and brittle characteristics, such as the Nomex honeycomb. Therefore, the holding method based on the false boss connection can be utilized due to its advantages of low cost, less pollution, and a short preparation period. In this study, the method to determine and optimize the critical parameters of the false boss design is proposed to address the issue that they previously relied heavily on the experience of engineers, which often results in much waste of materials. To determine the critical parameters, a simulation model is constructed for Nomex honeycomb core parts machining with a false boss holding. Based on the simulation model, the stability of the machining process is analyzed, and the weak link of the false boss between different milling areas is studied. Furthermore, the difference in the shape of different parts is considered, and the reasonable critical parameters of the false boss are obtained through analysis. [ABSTRACT FROM AUTHOR]

Obtaining river or sea pebbles from local resources for concrete production is considered an economical and eco-friendly alternative, particularly in marine and island-offshore engineering. However, the resulting changes in the mechanical properties of these concrete have attracted attention. This study investigates the compressive behavior of concretes where river or sea pebbles partially (i.e., 33% and 67%) or fully (i.e., 100%) replace traditional gravel as coarse aggregate, using a noncontact full-field deformation measurement system based on digital image correlation (DIC). Compared to the traditional gravel concrete (GC), compressive strengths of the river pebble concrete (RPC) at constitution rates of 33%, 67%, and 100% decreased by 6.5%, 29.8%, and 38.9% while those values of the sea pebble concrete (SPC) decreased by 13.1%, 32.7%, and 44.3%, respectively. Meanwhile, SPC exhibited slightly lower compressive strength than RPC. The peak strains of both SPC and RPC decreased at lower substitution rates, although their stress-strain curves resembled those of GC. In contrast, RPC and SPC at higher substitution rates exhibited a noticeable stage of load hardening. Full-field deformation data and interfacial characteristics indicated that the compressive failure modes of both RPC and SPC showed significant interfacial slipping between pebbles and mortar with increasing coarse aggregate substitution rates. In comparison, fractures in coarse aggregate and mortar were observed in damaged GC. The study demonstrated that the spatio-temporal compressive deformation response and failure modes of SPC and RPC were distinct due to the introduction of pebbles, providing insights for engineering applications of river/sea pebble concrete in practical offshore or island construction projects. [ABSTRACT FROM AUTHOR]

Acute respiratory distress syndrome (ARDS) is a critical illness in critically unwell patients, characterized by refractory hypoxemia and shock. This study evaluates an early detection tool and investigates the relationship between hypoxia and circulatory shock in ARDS, to improve diagnostic precision and therapy customization. We used a porcine model, inducing ARDS with mechanical ventilation and intratracheal plus intravenous lipopolysaccharide (LPS) injection. Hemodynamic changes were monitored using an Acumen IQ sensor and a ForeSight Elite sensor connected to the HemoSphere platform. We evaluated tissue damage, inflammatory response, and hypoxia-inducible factor (HIF) alterations using enzyme-linked immunosorbent assay and immunohistochemistry. The results showed severe hypotension and increased heart rates post-LPS exposure, with a notable rise in the hypotension prediction index (HPI) during acute lung injury (p = 0.024). Tissue oxygen saturation dropped considerably in the right brain region. Interestingly, post-injury HIF-2α levels were lower at the end of the experiment. Our findings imply that the HPI can effectively predict ARDS-related hypotension. HIF expression levels may serve as possible markers of rapid ARDS progression. Further research should be conducted on the clinical value of this novel approach in critical care, as well as the relationship between the HIF pathway and ARDS-associated hypotension. [ABSTRACT FROM AUTHOR]

Gouty arthritis (GA) is an inflammatory disease caused by monosodium urate (MSU) crystals deposited in the joint tissues causing severe pain. The disease can recur frequently and tends to form tophus in the joints. Current therapeutic drugs for the acute phase of GA have many side effects and limitations, are unable to prevent recurrent GA attacks and tophus formation, and overall efficacy is unsatisfactory. Therefore, we need to advance research on the microscopic mechanism of GA and seek safer and more effective drugs through relevant targets to block the GA disease process. Current research shows that the pathogenesis of GA is closely related to NLRP3 inflammation, oxidative stress, MAPK, NET, autophagy, and Ferroptosis. However, after synthesizing and sorting out the above mechanisms, it is found that the presence of ROS is throughout almost the entire spectrum of micro-mechanisms of the gout disease process, which combines multiple immune responses to form a large network diagram of complex and tight connections involved in the GA disease process. Current studies have shown that inflammation, oxidative stress, cell necrosis, and pathological signs of GA in GA joint tissues can be effectively suppressed by modulating ROS network-related targets. In this article, on the one hand, we investigated the generative mechanism of ROS network generation and its association with GA. On the other hand, we explored the potential of related targets for the treatment of gout and the prevention of tophus formation, which can provide effective reference ideas for the development of highly effective drugs for the treatment of GA. [ABSTRACT FROM AUTHOR]

VW63Z magnesium alloy, known for its high strength-to-weight ratio and excellent mechanical properties, is a promising candidate for applications in the automotive industries. Among the anti-corrosion technologies for VW63Z alloys, organic electrophoretic coatings have gained significant attention due to their ability to provide a uniform and controlled coating thickness, high coating adhesion, and excellent corrosion protection properties. In this work, to investigate the aging behaviors of an organic electrophoretic coating for magnesium alloys, water absorption kinetics curves of the coatings after immersion in 3.5 wt.% NaCl at 20 °C and 40 °C were measured, and parameters such as the saturated water absorption and saturation time of the free film coatings were fitted to determine the densification evolution of the organic coatings. A tensile test was used to test the tensile strength of the organic coating during service to determine the evolution of the strength and toughness of the organic coating. The evolution of wet adhesion of organic coatings with service time was tested by ASTM D4541-02. The impedance spectra of the organic coatings under the above conditions were detected by EIS, and finally, the failure behavior of the organic coatings under immersion was analyzed. [ABSTRACT FROM AUTHOR]

Nafion membranes are widely used as proton exchange membranes, but their proton conductivity deteriorates in high-temperature environments due to the loss of water molecules. To address this challenge, we propose the utilization of porous aromatic frameworks (PAFs) as a potential solution. PAFs exhibit remarkable characteristics, such as a high specific surface area and porosity, and their porous channels can be post-synthesized. Here, a novel approach was employed to synthesize a PAF material, designated as PAF-45D, which exhibits a specific surface area of 1571.9 m2·g−1 and possesses the added benefits of facile synthesis and a low cost. Subsequently, sulfonation treatment was applied to PAF-45D in order to introduce sulfonic acid groups into its pores, resulting in the formation of PAF-45DS. The successful incorporation of sulfonic groups was confirmed through TG, IR, and EDS analyses. Furthermore, a novel Nafion composite membrane was prepared by incorporating PAF-45DS. The Nyquist plot of the composite membranes demonstrates that the sulfonated PAF-45DS material can enhance the proton conductivity of Nafion membranes at high temperatures. Specifically, under identical film formation conditions, doping with a 4% mass fraction of PAF-45DS, the conductivity of the Nafion composite membrane increased remarkably from 2.25 × 10−3 S·cm−1 to 5.67 × 10−3 S·cm−1, nearly 2.5 times higher. Such promising and cost-effective materials could be envisioned for application in the field of Nafion composite membranes. [ABSTRACT FROM AUTHOR]

The gut microbiota plays an important role in host nutrient absorption, immune function, and behavioral patterns. Much research on the gut microbiota of wildlife has focused on feces samples, so the microbial composition along the gastrointestinal tract of wildlife is not well reported. To address this gap, we performed high-throughput sequencing of 16s rRNA genes and ITs rRNA genes in the gastrointestinal contents of a wild adult male Chinese muntjac (Muntiacus reevesi) to comparatively analyze the microbial diversity of different gastrointestinal regions. The results showed that the dominant bacterial phyla were Firmicutes (66.19%) and Bacteroidetes (22.7%), while the dominant fungal phyla were Ascomycetes (72.81%). The highest bacterial diversity was found in the stomach, and the highest fungal diversity was found in the cecum. The microbial communities of the large intestine and small intestine were of similar structures, which were distinct from that of the stomach. These results would facilitate the continued exploration of the microbial composition and functional diversity of the gastrointestinal tract of wild Chinese muntjacs and provide a scientific basis for microbial resource conservation of more wildlife. [ABSTRACT FROM AUTHOR]