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In the maize-soybean intercropping system, varying degrees of maize leaf shading are an important factor that reduces the uniformity of light penetration within the soybean canopy, altering the soybean canopy structure. Quantitative analysis of the relationship between the soybean canopy structure and canopy photosynthesis helps with breeding shade-tolerant soybean varieties for intercropping systems. This study examined the canopy structure and photosynthesis of intercropped soybeans during the shading stress period (28 days before the corn harvest), the high light adaptation period (15 days after the corn harvest), and the recovery period (35 and 55 days after the corn harvest), using a field high-throughput phenotyping platform and a plant gas exchange testing system (CAPTS). Additionally, indoor shading experiments were conducted for validation. The results indicate that shade-tolerant soybean varieties (STV varieties) have significantly higher yields than shade-sensitive soybean varieties (SSV varieties). This is attributable to the STV varieties having a larger top area, lateral width, and lateral external rectangular area. Compared to the SSV varieties, the four top areas of the STV varieties are, on average, 52.09%, 72.05%, and 61.37% higher during the shading stress, high light adaptation, and recovery periods, respectively. Furthermore, the average maximum growth rates (GRs) for the side mean width (SMW) and side rectangle area (SRA) of the STV varieties are 62.92% and 22.13% in the field, and 83.36% and 55.53% in the indoor environment, respectively. This results in a lower canopy overlap in STV varieties, leading to a more uniform light distribution within the canopy, which is reflected in higher photosynthetic rates (Pn), apparent quantum efficiency, and whole-leaf photosynthetic potential (WLPP) for the STV varieties, thereby enhancing their adaptability to shading stress. Above-ground dry matter accumulation was higher in STV varieties, with more assimilates stored in the source and sink, promoting assimilate accumulation in the grains. These results provide new insights into how the superior canopy structure and photosynthesis of shade-tolerant soybean varieties contribute to increased yield. [ABSTRACT FROM AUTHOR]

Although travelers are frequently accompanied by abdominal discomfort and even diarrhea, not every trip can cause this issue. Many studies have reported that intestinal microbes play an important role in it. However, little is known about the reason for the dynamics of these intestinal microbes. Here, we delved into the effects of short-term travel on the gut microbiota of 12 healthy individuals. A total of 72 fecal samples collected before and after one-week travel, alongside non-traveling controls, underwent amplicon sequencing and a series of bioinformatic analyses. We found that travel significantly increased intra-individual gut microbiota fluctuations without diarrhea symptoms. In addition, the initial composition of the gut microbiota before travel emerged as a crucial factor in understanding these fluctuations. Travelers with stable microbiota exhibited an enrichment of specific probiotic bacteria (Agathobaculum, Faecalibacterium, Bifidobacterium, Roseburia, Lactobacillus) before travel. Another batch of data validated their predictive role in distinguishing travelers with and without the gut microbial disorder. This work provided valuable insights into understanding the relationship between gut microbiota and travel. It offered a microbiota-centric perspective and a potential avenue for interventions to preserve gut health during travel. [ABSTRACT FROM AUTHOR]

Crops often face light intensity fluctuations in natural settings. Intercropping is widely used to improve crop yield and resource utilization worldwide, but crops suffer from high-frequency and high-intensity light fluctuations due to mutual crop influence. Soybean is an important legume crop and is often intercropped with other crops, but little is known about soybean's response to light fluctuation environments. Herein, three fluctuation frequencies (1, 10, and 20 min/cycle) were used to analyze soybean photosynthesis responses by measuring leaf growth, chlorophyll content, gas exchange, and electron transfer. Our data revealed that faster fluctuation frequencies led to the stronger suppression of soybean morphology and photosynthesis, with significant reductions of 31.31% and 21.58%, respectively. Damage to photosystems II (PSII) and I (PSI) also intensified, with significant decreases of 18.52% and 18.38% in their effective quantum yields Y(II) and Y(I). Additionally, increased fluctuation frequency exacerbated the consumption of the plastoquinone pool and linear electron flow but enhanced the cyclic electron flow across the thylakoid membrane and, thus, increased heat dissipation in PSII. Our findings indicate that an increased fluctuation frequency inflicted more severe damage on the soybean photosynthesis system. However, PSI-enhanced CEF improved NPQ and coordinated photoprotection to some extent. [ABSTRACT FROM AUTHOR]

Piezoelectric material-based devices have garnered considerable attention from scientists and engineers due to their unique physical characteristics, resulting in numerous intriguing and practical applications. Among these, flexural-mode piezoelectric resonators (FMPRs) are progressively gaining prominence due to their compact, precise, and efficient performance in diverse applications. FMPRs, resonators that utilize one- or two-dimensional piezoelectric materials as their resonant structure, vibrate in a flexural mode. The resonant properties of the resonator directly influence its performance, making in-depth research into the resonant characteristics of FMPRs practically significant for optimizing their design and enhancing their performance. With the swift advancement of micro-nano electronic technology, the application range of FMPRs continues to broaden. These resonators, representing a domain of piezoelectric material application in micro-nanoelectromechanical systems, have found extensive use in the field of physical sensing and are starting to be used in micropower systems and biomedicine. This paper reviews the structure, working principle, resonance characteristics, applications, and future prospects of FMPRs. [ABSTRACT FROM AUTHOR]

Background: Caspofungin is an echinocandin antifungal agent commonly used as the first-line therapy for invasive candidiasis, salvage therapy for invasive aspergillosis, and empirical therapy for presumed fungal infections. Pharmacokinetic (PK) variabilities and suboptimal exposure have been reported for caspofungin, increasing the risk of insufficient efficacy. Objective: This work aimed to develop a caspofungin population pharmacokinetic (popPK) library and demonstrate its utility by assessing the probability of target attainment across diverse settings. Methods: We established a caspofungin popPK model library following a rigorous literature review, re-implementing selected models in R with rxode2. Quality control procedures included a comparison of different studies and assessing covariate impacts. Model libraries were primarily used to perform Monte Carlo simulations to estimate target attainment and guide personalized dosing in Candida infections. Results: A total of 13 models, one- or two-compartment models, were included. The most significant covariates were body size (weight and body surface area), liver function, and albumin level. The results show that children and adults showed considerable differences in pharmacokinetics. For C. albicans and C. parapsilosis, none of the populations achieved a PTA of ≥90% at their respective susceptible MIC values. In contrast, for C. glabrata, 70% of the adult studies reached a PTA of ≥90%, while all pediatric studies achieved the same PTA level. Conclusion: At the recommended dosage, adult patients showed notably lower exposure to caspofungin compared to pediatric patients. Considering body size, liver function, and serum albumin is crucial when determining caspofungin dosage regimens. Furthermore, further research is required to comprehensively understand the pharmacokinetics of caspofungin in pediatric patients. [ABSTRACT FROM AUTHOR]

In order to rapidly breed high-quality varieties, an increasing number of plant researchers have identified the functions of a large number of genes, but there is a serious lack of research on plants' phenotypic traits. This severely hampers the breeding process and exacerbates the dual challenges of scarce resources and resource development and utilization. Currently, research on crop phenotyping has gradually transitioned from traditional methods to HTP technologies, highlighting the high regard scientists have for these technologies. It is well known that different crops' phenotypic traits exhibit certain differences. Therefore, in rapidly acquiring phenotypic data and efficiently extracting key information from massive datasets is precisely where HTP technologies play a crucial role in agricultural development. The core content of this article, starting from the perspective of crop phenomics, summarizes the current research status of HTP technology, both domestically and internationally; the application of HTP technology in above-ground and underground parts of crops; and its integration with precision agriculture implementation and multi-omics research. Finally, the bottleneck and countermeasures of HTP technology in the current agricultural context are proposed in order to provide a new method for phenotype research. HTP technologies dynamically monitor plant growth conditions with multi-scale, comprehensive, and automated assessments. This enables a more effective exploration of the intrinsic "genotype-phenotype-environment" relationships, unveiling the mechanisms behind specific biological traits. In doing so, these technologies support the improvement and evolution of superior varieties. [ABSTRACT FROM AUTHOR]

Simple Summary: Temperature is an important factor that affects the growth and reproduction of insects. The silkworm is an economically important insect, and as a representative insect of Lepidoptera, it is particularly important to investigate its extreme-temperature resistance mechanisms. In this article, we identified a gene in B. mori, the B. mori singed (Bmsn) gene, which is involved in the high-temperature resistance of silkworms. We demonstrated that the Bmsn gene can increase the proliferation activity of silkworm cells and enhance their tolerance to high temperatures. Furthermore, we constructed a transgenic B. mori strain that overexpressed the Bmsn gene and demonstrated that its overexpression can increase high-temperature resistance and improve the economic characteristics of silkworms. Temperature is an important factor in the growth, development, survival, and reproduction of organisms. The high-temperature resistance mechanism of insects may be significant for use in the prevention and control of insect pests. The silkworm, Bombyx mori, is an important Lepidoptera model species for studies on pest control in agriculture and forestry. We identified a gene in B. mori, the B. mori singed (Bmsn) gene, which is involved in the high-temperature resistance of silkworms. Sn proteins are highly conserved among species in many taxonomic groups. The overexpression of the Bmsn gene promoted the proliferation of silkworm cells, reduced oxidation, and reduced the accumulation of reactive oxygen species under stress. Interfering with the Bmsn gene had the opposite result. We constructed a transgenic B. mori strain that overexpressed the Bmsn gene. The physiological traits of the transgenic strain were significantly improved, and it had stronger high-temperature resistance. The Bmsn gene is involved in the process by which fat bodies respond to high-temperature stress. These findings provide insights into the mechanism of high-temperature resistance of insects and offer a new perspective on agricultural and forestry pest control. [ABSTRACT FROM AUTHOR]

In order to reveal the yield-increasing mechanism of relay intercropping (RI) maize with different varieties from the perspective of plant growth, source sink relationship, and root growth, a two-factor randomized block design trial was designed, which includes different maize varieties (Rongyu1210 (RY1210), Zhongyu 3 (ZY3)) and plant pattern (RI, Sole cropping (SC)). The leaf area index (LAI), dry matter accumulation and distribution, root dry weight (RDW), root length (RL), root surface area (RSA), root volume (RV), and maize yield were determined. LAI of RI RY1210 was significantly higher than that of the SC RY1210 at the filling stage and maturity stage. The dry matter accumulation of RI RY1210 ear was significantly higher than that of SC RY1210 and RI ZY3, and the RDW of RY1210 was significantly higher than that of ZY3. The ratio of RDW of RI RY1210 was higher than that of RI ZY3 in the 20–40 and 40–60 cm soil layers, respectively. The RDW, RL, RV, and RSA of RI RY1210 were significantly lower than that of sole RY1210 by 25.43%, 10.75%, 30.79%, and 23.73%, respectively, but higher than that of RI ZY3 by 143.98%, 278.29%, 54.40%, and 29.57%, respectively. The average yield of RI RY1210 was 8782.71 kg ha−1, with no significant difference compared to SC, which was mainly attributed to a larger ear dry matter accumulation, higher LAI in later growth stages, larger RDW, and the ratio of roots in deeper soil layers. This study will be useful and helpful to farmers for how to select and plant high-yielding maize varieties in strip relay intercropping. [ABSTRACT FROM AUTHOR]

Fitting probability distribution functions to observed data is the standard way to compute future design floods, but may not accurately reflect the projected future pattern of extreme events related to climate change. In applying the latest coupled model intercomparison project (CMIP5 and CMIP6), this research investigates how likely it is that precipitation changes in CMIP5 and CMIP6 will affect both the magnitude and frequency of flood analysis. GCM output from four modelling institutes in CMIP5, with representative pathway concentration (RCP8.5) and the corresponding CMIP6 shared socioeconomic pathways (SSP585), were selected for historical and future periods, before the project precipitation was statistically downscaled for selected cities by using delta, quantile mapping (QM), and empirical quantile mapping (EQM). On the basis of performance evaluation, a rainfall-runoff hydrological model was developed by using the stormwater management model (SWMM) for CMIPs (CMIP5 and CMIP6) in historical and future horizons. The results reveal an unprecedented increase in extreme events, for both CMIP5 (historical) and CMIP6 (future) projections. The years 2070–2080 were identified by both CMIP5 and CMIP6 as experiencing the most severe flooding. [ABSTRACT FROM AUTHOR]

Ferroelectret nanogenerator (FENG), renowned for its remarkable electromechanical conversion efficiency and low Young's modulus, has gained significant attention in various acoustic applications. The increasing interest is attributed to the crucial role acoustic devices play in our daily lives. This paper provides a comprehensive review of the advancements made in using FENG for acoustic applications. It elaborates on the operational mechanism of FENG in acoustics, with a special focus on comparing the influence of different fabrication materials and techniques on its properties. This review categorizes acoustic applications of FENG into three primary areas: acoustic sensing, acoustic actuation, and acoustic energy harvesting. The detailed descriptions of FENG's implementations in these areas are provided, and potential directions and challenges for further development are outlined. By demonstrating the wide range of potential applications for FENG, it is shown that FENG can be adapted to meet different individual needs. [ABSTRACT FROM AUTHOR]

Soybean mosaic virus (SMV) seriously harms soybean quality and yield. In order to understand the effect of a heterogeneous light environment on the disease resistance of intercropped soybeans, we simulated three kinds of light environments to learn the effects of white light, blue light, and far-red light on the SMV resistance of soybeans. The results showed that compared with the control, SMV-infected soybeans showed dwarfing and enhanced defense. The symptoms of leaves under red and blue light were less severe than those under white light, the virus content of infected plants was about 90% lower than under white light, the activity of antioxidant enzymes increased, and the accumulation of reactive oxygen species decreased. The oxidation damage in SMV-infected soybeans was serious under far-red light. Transcriptome data showed that the biostimulatory response, plant–pathogen interaction, and plant hormone signaling pathway gene expression of SMV-infected soybeans were significantly up-regulated under red light compared with the control. Compared with the control, the genes in the biostimulatory response, calcium ion binding, carbohydrate-binding, mitogen-activated protein kinase (MAPK) signaling, and plant–pathogen interaction pathways, were significantly up-regulated in SMV-infected soybeans under blue light. In far-red light, only 39 genes were differentially expressed in SMV-infected soybeans compared with the control, and most of the genes were down-regulated. Compared with the control, the up-regulation of the salicylic acid (SA) pathway defense gene in SMV-infected soybeans under red light was higher than under other light treatments. Compared with the control, the up-regulation of the jasmonic acid (JA) and ethylene (ET) pathway defense genes in SMV-infected soybeans under blue light was higher than under other light treatments. Compared with the control, most defense-related genes in the SA and JA pathways were inhibited in SMV-infected soybeans under far-red light, while genes in the ET pathway were significantly up-regulated. These results will advance our understanding of the disease resistance mechanism of intercropping soybeans in a heterogeneous light environment and provide new ideas for the prevention and control of viral diseases. [ABSTRACT FROM AUTHOR]

Okra flowers are a good source of polysaccharides and flavonoids, with biological activities of anti-inflammatory action and modulation of the gut microbiota. Previously, we reported that flavonoid-rich extracts from okra flowers (AFE) presented effective anti-colorectal cancer (CRC) activity in CRC cells as well as xenograft models, but their role in colitis-associated cancer (CAC) is unidentified. In this study, we aimed to evaluate the effects of AFE and APE (polysaccharides extracted from okra flowers) on the CAC symptoms of azoxymethane (AOM)/dextran sodium sulfate (DSS)-intervened mice. The results showed that APE and AFE exert potent efficacy in inhibiting colitis and colorectal tumorigenesis stimulated by AOM/DSS, characterized by decreased colonic shortening, DAI score, and tumor numbers. Compared with the control group, APE/AFE alleviated the microbiota dysbiosis driven by AOM/DSS. In addition, AFE elicited its anticancer activity through regulation of NFκB/IL-6/Stat3, JAK2/Stat3, MAPKs, PI3K/AKT, and Wnt/β-catenin signal transductions in AOM/DSS mice, which was consistent with a vitro model of CT26 cells, while APE treatment exhibited anticancer activity through regulation of Nrf2/IL-6, MAPKs, PI3K/AKT, and Wnt/β-catenin signal transductions in the AOM/DSS mouse model. Collectively, our studies revealed, for the first time, that flavonoids and polysaccharides from okra flowers possess the ability to attenuate colitis and colorectal tumorigenesis, with them having great potential to become promising candidates against CRC. [ABSTRACT FROM AUTHOR]

The plant architecture of higher plants is regulated through environmental and genetic factors, as well as phytohormones. Phytohormones play a critical role in regulating shoot branching. We determined the branching phenotype of D16 and N99-6, the content of strigolactones, the genetic expression level, and the interaction between auxin and strigolactones. We found that the branching development of the two soybean varieties under shading was significantly slower than that under normal light. The average branch length of N99-6 decreased by 40.9% after shading; however, the branch length of D16 was not significantly affected. Meanwhile, the branch formation rate in D16 was significantly higher than in N99-6. In addition, after shading treatment, the content of strigolactones in D16 and N99-6 axillary buds increased significantly, and the expression of phytochrome genes, PhyA and PhyB, showed opposite changes. However, strigolactone synthesis gene GmMAX4 and signal transduction gene GmMAX2 expression levels of D16 were lower than those of N99-6 after 24 h of shading. In addition, the application of strigolactone inhibitor TIS108 and auxin inhibitor NPA to soybean had no significant effect on the branch phenotype. The expression of the GmMAX2 gene was significantly up-regulated after the external application of the auxin analog, and the expression of auxin transporter gene GmPINI was significantly down-regulated after external application of the strigolactone analog under shade. In this study, we investigated the adverse effect of shade on soybean branching development, which may be due to the interaction of strigolactones with auxins. [ABSTRACT FROM AUTHOR]

Background: Ganciclovir and valganciclovir are used for prophylaxis and treatment of cytomegalovirus infection. However, there is great interindividual variability in ganciclovir's pharmacokinetics (PK), highlighting the importance of individualized dosing. To facilitate model-informed precision dosing (MIPD), this study aimed to establish a parametric model repository of ganciclovir and valganciclovir by summarizing existing population pharmacokinetic information and analyzing the sources of variability. (2) Methods: A total of four databases were searched for published population PK models. We replicated these models, evaluated the impact of covariates on clearance, calculated the probability of target attainment for each model based on a predetermined dosing regimen, and developed an area under the concentration–time curve (AUC) calculator using maximum a posteriori Bayesian estimation. (3) Results: A total of 16 models, one- or two-compartment models, were included. The most significant covariates were body size (weight and body surface area) and renal function. The results show that 5 mg/kg/12 h of ganciclovir could make the AUC0–24h within 40–80 mg·h/L for 50.03% pediatrics but cause AUC0–24h exceeding the exposure thresholds for toxicity (120 mg·h/L) in 51.24% adults. (4) Conclusions: Dosing regimens of ganciclovir and valganciclovir should be adjusted according to body size and renal function. This model repository has a broad range of potential applications in MIPD. [ABSTRACT FROM AUTHOR]

Soybean in relay intercropping is initially exposed to a shade environment, followed by exposure to full sunlight after the harvesting of primary crops, e.g., maize. Therefore, soybean's ability to acclimate to this changing light environment determines its growth and yield formation. However, the changes in soybean photosynthesis under such light alternations in relay intercropping are poorly understood. This study compared the photosynthetic acclimation of two soybean varieties with contrasting shade tolerance, i.e., Gongxuan1 (shade-tolerant) and C103 (shade-intolerant). The two soybean genotypes were grown in a greenhouse under full sunlight (HL) and 40% full sunlight (LL) conditions. Subsequently, after the fifth compound leaf expanded, half of the LL plants were transferred to a high-sunlight environment (LL-HL). Morphological traits were measured at 0 and 10 days, while chlorophyll content, gas exchange characteristics and chlorophyll fluorescence were assayed at 0, 2, 4, 7 and 10 days after transfer to an HL environment (LL-HL). Shade-intolerant C103 showed photoinhibition 10 days after transfer, and the net photosynthetic rate (Pn) did not completely recover to that under a high light level. On the day of transfer, the shade-intolerant variety, C103, exhibited a decrease in net photosynthetic rate (Pn), stomatal conductance (Gs) and transpiration rate (E) in the low-light (LL) and low-light-to-high-light (LL-HL) treatments. Additionally, intercellular CO2 concentration (Ci) increased in low light, suggesting that non-stomatal factors were the primary limitations to photosynthesis in C103 following the transfer. In contrast, the shade-tolerant variety, Gongxuan1, displayed a greater increase in Pn 7 days after transfer, with no difference observed between the HL and LL-HL treatments. Ten days after transfer, the shade-tolerant Gongxuan1 exhibited 24.1%, 10.9% and 20.9% higher biomass, leaf area and stem diameter than the intolerant C103. These findings suggest that Gongxuan1 possesses a higher capacity to adapt to variations in light conditions, making it a potential candidate for variety selection in intercropping systems. [ABSTRACT FROM AUTHOR]

To ensure yield in strip-intercropped maize with soybeans (SM), it is crucial to plant at a density comparable to that of monoculture maize (MM). This requires reducing spacing by more than half, increasing intraspecific competition, and altering stalk lodging resistance traits compared with MM. Nitrogen fertilization can effectively mediate stalk lodging resistance. However, it is still unclear how nitrogen rates influence SM's stalk lodging resistance under high-density conditions and how that resistance compares to MM. The experiment involved four N fertilizer treatments with two planting densities: medium density (60,000 plants/ha) and high density (75,000 plants/ha). Additionally, different planting patterns of strip-cropped (S) and monoculture (M) were implemented. The N fertilizer application rates were N0 (0 kg/ha), N225 (225 kg/ha), N300 (300 kg/ha), and N375 (375 kg/ha). The stalk lodging resistance was represented by the breaking strength of the third basal internode. The study revealed that, at the same planting density, the third basal internode of the stalk exhibited consistent results in terms of its diameter, crushing strength, total number and area of vascular bundles, and N content. Notably, all these traits exhibited a significant positive relationship with breaking strength. The highest values for these parameters and yield were observed under N225 and N300 fertilization rates for medium-density monoculture and strip-cropped maize, respectively. In contrast, the high-density monoculture and strip-cropped maize showed peak performance under N300 and N375 fertilization rates. At both medium and high planting densities, the strip-cropped maize exhibited 8.9% and 10.9% lower breaking strength than the monoculture maize under N225 treatment. However, increasing the N fertilizer application resulted in comparable lodging resistance between the strip-cropped maize and the maximum values of the monoculture maize, at N300 treatment for medium density and N375 treatment for high density. Hence, strip-cropped maize planted at high density (75,000 plants/ha) with a lower nitrogen rate had lower lodging resistance than monoculture maize, but it can be improved to match the monoculture maize by increasing the nitrogen rate. [ABSTRACT FROM AUTHOR]

Under the state's key surveillance, petrochemical industries are considered polluting enterprises. Even though large-scale petrochemical enterprises follow the complete treatment of combustion waste gas, process waste gas, and volatile organic waste gas pollutants, the impact of pollutant emissions on the regional air quality is unclear. This study used the atmospheric chemical transport model and adopted the subtraction method to simulate the impacts of air pollutant emissions from four typical petrochemical enterprises on regional air quality of the North China Plain. Results indicated that emissions from petrochemical enterprises on surface PM2.5, SO2, and NO2 concentrations mainly contributed to the nearby area, particularly SO2 and NO2. The pollution can be controlled within the boundaries of the petrochemical plants. Petrochemical enterprises had a small SO2 and NO2 contribution with a maximum of up to 4.65% within a 9 km distance. Emissions from petrochemical enterprises contributed less to surface PM2.5 concentrations (less than 0.5%) within a 9 km distance. Surface O3 concentrations driven by petrochemical enterprises did not show near-source distribution characteristics, which were closely related to its complex precursors and secondary reactions. Contributions of petrochemical enterprises to local pollution decreased significantly with the increase in distance. The SO2 and NO2 pollution contributions to the North China Plain remained around 0.1–0.2%, with the maximum contribution occurring in January and July. The maximum contribution of PM2.5 in this region was in April (0.42%) while it was below 0.1% for other months. The pollutant emission from the four typical petrochemical enterprises in the North China Plain had little impact on the concentration of air pollutants in the North China Plain. However, it had a significant impact on the ambient air quality in the region near the enterprise. This study can be useful in analyzing and refining the influence of enterprises on the region. [ABSTRACT FROM AUTHOR]

Blue and red light are essential light signals used to regulate stomatal development and leaf structure. In the present study, stomatal and leaf traits that respond to blue and red light were studied at two light intensities (400 and 100 µmol m−2 s−1) in soybeans. The stomatal traits and leaf characteristics were determined. Furthermore, their contribution to the operational maximum stomatal conductance (gopmax) was evaluated using the rdacca.hp R package. With the light intensity significantly reduced, the stomatal size (SZ) under blue light did not change. Similarly, the decrease in light intensity did not influence the stomatal density (SD), specific leaf weight (SLW) or gopmax under red light. These results implied that the regulation of SD and SLW depended on blue light and that SZ was highly sensitive to red light. In addition, SLW was strongly correlated with SD. The SLW and SD had the highest contribution rates (19.43% and 19.5%, respectively) to gopmax, as compared with the other parameters. In conclusion, these results suggested that in long-term exposure to blue light, the enhancements in gopmax were primarily due to the synergistic promotion of SLW and SD. [ABSTRACT FROM AUTHOR]

To construct functional photonic integrated circuits, an efficient and compact laser is expected to be incorporated into the complementary metal–oxide–semiconductor platform. Monolithic integration of III–V lasers on pre-patterned Si substrates by the aspect ratio trapping method is a promising solution. Here, microwires with reversed ridge waveguides (RRWs) on pre-patterned Si substrates were reported. By metal–organic chemical vapor deposition, high-quality InP microwires with RRWs were obtained, and InGaAs/InGaAsP multi-quantum-well structures with InGaAsP separate confinement hetero structure (SCH–MQW) were successfully achieved. The SCH–MQW structure was buried in the InP microwire, which was beneficial for transferring the heat generated in the active region. The micron size also contributes to the efficiency of thermal diffusion. Further, simulation results showed that the metal absorption loss could be less than 4 dB/cm by properly controlling the contact area between metal electrodes and microwires. This proposed structure opens up an alternative pathway for electrically driven III–V lasers seamlessly interfaced with Si-photonics. [ABSTRACT FROM AUTHOR]

Forage deficiency is the bottleneck that restricts the development of plateau animal husbandry. Maize (Zea mays L.)–soybean (Glycine max L.) intercropping can improve the forage biomass yield and silage quality. This experiment was conducted in Ganzi Tibetan Autonomous Prefecture to explore the effects of four maize varieties (M1, Rongyu Silage No. 1; M2, Yayu 04889; M3, Demeiya No. 1; M4, Zhenghong 505) on biomass yield, nutritional composition, and silage quality in maize–soybean intercropping. The results showed that M1S had the highest total dry matter yield (18.03 t ha−1), M3S had the highest crude protein (CP) content (8.46% DM), and soybeans had the highest water-soluble carbohydrate (WSC) content (8.55% DM). After silage, the CP content (13.44% DM) of mixed silage in M3S was higher, and the contents of neutral detergent fiber (39.42% DM) and acid detergent fiber (25.42% DM) were lower than those in maize silage alone. The WSC content (4.45% DM) of mixed silage in M3S was higher and the pH value (4.46) and ammonia–nitrogen to total nitrogen (3.97%) were lower than those of soybean silage alone. The results of membership function analysis showed that M3S was the best in fresh feeding and silage utilization, followed by M1S. Therefore, M3S (Demeiya No 1. intercropped with soybeans) is recommended in high-altitude areas. [ABSTRACT FROM AUTHOR]

The flower and pod abscission is one of the characteristics of soybean that severely limits yield, especially when intercropped with maize. Therefore, suitable soybean cultivars for intercropping are urgently needed to improve farmland productivity. We conducted a two-year field experiment to evaluate the flower and pod abscission, dry matter production, and yield advantages of 15 soybean cultivars. The results of the principal component analysis (PCA) and cluster analysis (CA) showed that 15 soybean cultivars were classified into three groups, i.e., high-yielding group (HYG), mid-yielding cultivars (MYG), and low-yielding cultivars (LYG). In the HYG group, ND12 and GX3 had characteristics of more flowers and pods and less abscission of flowers and pods. Moreover, the net assimilation rate (NAR) and relative growth rate (RGR) of HYG were significantly higher than the other. The HYG obtained a considerably higher partition ratio of 53% from biomass to seed than the other. Therefore, selecting and breeding cultivars with the characteristics of more flowers and pods and less abscission of flowers and pods can help to increase soybean yield in intercropping. [ABSTRACT FROM AUTHOR]

SMXL6,7,8 are important target proteins in strigolactone (SL) signal pathway, which negatively regulate the reception and response of SL signal, and play an important role in regulating plant branching. However, there is a relative lack of research on soybean SMXL gene family. In this study, 31 soybean SMXL genes were identified by phylogenetic analysis and divided into three groups. Based on the analysis of GmSMXL gene's structure and motif composition, it was found that the GmSMXL members in the same group were similar. The results of cis-element analysis showed that GmSMXL genes may regulate the growth and development of soybean by responding to hormones and environment. Based on the tissue specificity analysis and GR24 treatment, the results showed that four GmSMXLs in G1 group were predominantly expressed in stems, axillary buds and leaves and involved in SL signal pathway. Finally, under shading stress, the expression of four genes in G1 group was slightly different in different varieties, which may be the reason for the difference in branching ability of different varieties under shading stress. We have systematically studied the SMXL gene family in soybean, which may lay a foundation for the study of the function of GmSMXL gene in the future. [ABSTRACT FROM AUTHOR]

Soil organic matter (SOM), soil aggregates, and soil microbes play key roles in agriculture soil fertility. In intercropping systems, the influences of straw incorporation and N input on the dynamics of soil physicochemical and microbial properties and their relationships are still unclear. We explore the changes in soil physicochemical and microbial properties with two straw managements, i.e., wheat straw incorporation (SI) and straw removal (SR), and four N supply rates for intercropped soybean, i.e., 60 (N60), 30 (N30), 15 (N15), and 0 (N0) kg N ha−1, in the wheat–maize–soybean relay strip intercropping systems. The results showed that SOM and SOM fractions contents, soil macroaggregate stability, and microbial and fungal α-diversity, e.g., Chao1 and Shannon indices, increased through straw incorporation and N input. The α-diversity was significantly positively correlated with soil physicochemical characteristics. Compared with SR, the relative abundance of ActinobacteriaandMortierellomycota in SI increased, but the relative abundance of Proteobacteria, Acidobacteria, and Ascomycota in SI decreased. In SI treatment, soil physicochemical characteristics and microbial diversity improved through N input, but that difference was not significant between N60 and N30. In conclusion, SI+N30 was the most effective way to maintain soil fertility and reduce the N fertilizer input in the wheat–maize–soybean relay strip intercropping. [ABSTRACT FROM AUTHOR]

In this investigation, 1060Al/AZ31B welded joints were obtained by magnetic pulse welding technique. In order to test the microstructure and mechanical properties of the joints, the welded joints were annealed at different temperatures and then examined by optical microscopy (OM), scanning electron microscopy (SEM), energy spectrum analysis (EDS) and mechanical properties testing. The testing results of the welded joints annealed at different temperatures showed that the Al-Mg MPW welded joints were well bonded. The changing of the microstructure and mechanical properties of Al/Mg welded joints was not apparent under the temperature of 200 °C. However, Al12Mg17 intermetallic compound layer formed at 200 °C. Al12Mg17 and Al3Mg2 intermetallic compound layers formed at the temperature of 300 °C. The diffusion rate of Mg and Al elements is proportional to the annealing temperature and the intermetallic compounds layer is gradually formed. The microhardness near the interface decreased first and then increased on account of the brittleness of intermetallic compounds. In the tensile shear tests, the fracture mechanism of Al/Mg MPW welded joints were analyzed. When the temperature was lower than 200 °C the joints did not crack. At 200 °C and 250 °C, the joints fracture along the Al12Mg17-Al interface. The joint cracks along the interface of Al12Mg17-Al3Mg2 at the temperature of 300 °C. [ABSTRACT FROM AUTHOR]

Feature matching is a fundamental procedure in several image processing methods applied in remote sensing. Multispectral sensors with different wavelengths can provide complementary information. In this work, we propose a multispectral line segment matching algorithm based on phase congruency and multiple local homographies (PC-MLH) for image pairs captured by the cross-spectrum sensors (visible spectrum and infrared spectrum) in man-made scenarios. The feature points are first extracted and matched according to phase congruency. Next, multi-layer local homographies are derived from clustered feature points via random sample consensus (RANSAC) to guide line segment matching. Moreover, three geometric constraints (line position encoding, overlap ratio, and point-to-line distance) are introduced in cascade to reduce the computational complexity. The two main contributions of our work are as follows: First, compared with the conventional line matching methods designed for single-spectrum images, PC-MLH is robust against nonlinear radiation distortion (NRD) and can handle the unknown multiple local mapping, two common challenges associated with multispectral feature matching. Second, fusion of line extraction results and line position encoding for neighbouring matching increase the number of matched line segments and speed up the matching process, respectively. The method is validated using two public datasets, CVC-multimodal and VIS-IR. The results show that the percentage of correct matches (PCM) using PC-MLH can reach 94%, which significantly outperforms other single-spectral and multispectral line segment matching methods. [ABSTRACT FROM AUTHOR]

Leaf veins constitute the transport network for water and photosynthetic assimilates in vascular plants. The class III homeodomain-leucine zipper (HD-Zip III) gene family is central to the regulation of vascular development. In this research, we performed an overall analysis of the HD-Zip III genes in soybean (Glycine max L. Merr.). Our analysis included the phylogeny, conservation domains and cis-elements in the promoters of these genes. We used the quantitative reverse transcription-polymerase chain reaction to characterize the expression patterns of HD-Zip III genes in leaf vein development and analyze the effects of exogenous hormone treatments. In this study, twelve HD-Zip III genes were identified from the soybean genome and named. All soybean HD-Zip III proteins contained four highly conserved domains. GmHB15-L-1 transcripts showed steadily increasing accumulation during all stages of leaf vein development and were highly expressed in cambium cells. GmREV-L-1 and GmHB14-L-2 had nearly identical expression patterns in soybean leaf vein tissues. GmREV-L-1 and GmHB14-L-2 transcripts remained at stable high levels at all xylem developmental stages. GmREV-L-1 and GmHB14-L-2 were expressed at high levels in the vascular cambium and xylem cells. Overall, GmHB15-L-1 may be an essential regulator that is responsible for the formation or maintenance of soybean vein cambial cells. GmREV-L-1 and GmHB14-L-2 were correlated with xylem differentiation in soybean leaf veins. This study will pave the way for identifying the molecular mechanism of leaf vein development. [ABSTRACT FROM AUTHOR]

The thermal environment of the development headings in deep underground mines tends to be harsh. The design of a reliable auxiliary ventilation system is an important strategy to maintain the thermal environment and air quality in the workplace. Hence, it is of great significance to comprehensively evaluate the effects of different air supply conditions and explore the optimal solution for the auxiliary ventilation system. In this study, cooling efficiency (CE) and ventilation efficiency (VE), which are the two most widely used indices for evaluating the ventilation in public places, were introduced into the field of underground mine ventilation. Numerical simulations of multiple air supply conditions were carried out to obtain the CE and VE, respectively. A data envelopment analysis model was used innovatively to evaluate and rank the efficiency of the different conditions. In the evaluation model, the air supply temperature and air supply velocity were the inputs, whereas CE and VE were the outputs. The preliminary results showed that cases with either the highest VE or highest CE alone may not actually indicate efficient air supply conditions. The optimum efficiency was determined to be at an air supply temperature difference of 6 °C. Thereafter, the air supply rate could be determined using the psychrometric chart based on the cooling and moisture loads. These results can provide theoretical guidance for optimizing air supply conditions and energy-saving control of mining ventilation and cooling systems. [ABSTRACT FROM AUTHOR]

Magnetic pulse welding (MPW) is widely used in the connection of dissimilar metals. The welding process involves the coupling of the electromagnetic field and structural field, which is a high-energy transient forming process. Based on the current experimental methods, it is difficult to capture the relevant data in the process of magnetic pulse welding, and the transient forming mechanism of magnetic pulse welding needs to be further studied. Taking the magnetic pulse welding of an Al-Mg sheet as an example, based on the Ansoft Maxwell and ANSYS finite element simulation platform, the loose coupling method was used to analyze an electromagnetic field generated by the discharging capacitor bank and structural field of the Al-Mg sheet under the action of electromagnetic force. The discharge period of the magnetic pulse welding capacitor bank was 62 μs. The current direction in the aluminum sheet changed once half a cycle, and the direction of the electromagnetic force was always consistent with the Z-axis. Under the skin effect, the magnetic induction intensity on the lower surface of the aluminum sheet was the largest. At 16 μs, the induced current, electromagnetic force and magnetic induction intensity in the aluminum sheet reached the peak values, which were 7.89 A/m2, 4.58 N/m3 and 12.6 T, respectively. The maximum electromagnetic force and velocity in the structural field were 2400 KN and 300 m/s. The structure field simulation reproduces the transient forming process of magnetic pulse welding, and clarifies the formation mechanism of the "intermediate zone rebound uncomposite zone-welding bonding zone-unbound zone". Based on the numerical simulation technology, the research on the transient forming mechanism of magnetic pulse welding under multiphysics simulations can promote the development and application of magnetic pulse welding technology and better guide engineering practices. [ABSTRACT FROM AUTHOR]

A two-year field experiment was conducted to address the effects of mixture composition and legume-grass seeding ratio on the biomass yield and nutritional quality of legume–grass mixtures. Alfalfa (Medicago sativa L.), white clover (Trifolium repens L.), red clover (Trifolium pratense L.), orchardgrass (Dactylis glomerata L.), perennial ryegrass (Lolium perenne L.), and tall fescue (Festuca arundinacea Schreb.) were selected as plant materials. A total of seven legume–grass mixtures (A1: white clover, orchardgrass, and tall fescue; A2: alfalfa, orchardgrass, and tall fescue; B1: alfalfa, white clover, orchardgrass, and tall fescue; B2: red clover, white clover, orchardgrass, and tall fescue; C1: alfalfa, white clover, orchardgrass, tall fescue, and perennial ryegrass; C2: red clover, white clover, orchardgrass, tall fescue, and perennial ryegrass; and D: alfalfa, red clover, white clover, orchardgrass, tall fescue, and perennial ryegrass) were sown in two legume-grass seeding ratios (L:G) of 4:6 and 5:5. The results showed that A2 produced a higher two-year average biomass yield (14.20 t/ha) in L:G of 4:6 than that of other mixtures. The grasses biomass yield proportion decreased while legume biomass yield proportion increased with prolonged establishment time. A2 showed a higher crude protein yield (2.5 t/ha) in L:G of 4:6. C2 and A1 showed lower neutral detergent fiber (4.6 t/ha) and acid detergent fiber (2.8 t/ha) yields in L:G 5:5, while diverse mixtures showed higher water-soluble carbohydrate yields. Overall, A2 showed a relative feed value of 146.50 in L:G of 4:6, indicating that it has not only produced the higher biomass yield but also had a better nutritional quality. [ABSTRACT FROM AUTHOR]

Intercropping is a cropping strategy that makes efficient use of space, nutrients, and soil. A 2-year field trial was conducted in 2019 and 2020 to study the effects of different soybean sowing times (9 days before corn sowing (ST1), 0 days at corn sowing (ST2), and 9 days after corn sowing (ST3), respectively) and densities (120,000 plants ha−1 (PD1), 150,000 plants ha−1 (PD2), and 180,000 plants ha−1 (PD3), respectively, and the planting density of corn was 60,000 plants ha−1 constantly) on total yield and on mixed silage quality in corn-soybean strip intercropping system. The yield decreased with an increase in soybean planting density. Before ensiling, the total dry matter (DM) content increased with an increase in soybean planting density, while that of crude protein content decreased with sowing time. The interaction of planting density × sowing time was significant for neutral detergent fiber and water-soluble carbohydrate (WSC) content. After ensiling, the WSC content of PD2ST3 (4.90% DM) was the highest. The PD1 (4.51%) had a higher content of ammonia–nitrogen to total nitrogen than that of PD2 and PD3. The lactic acid content of PD2ST3 (3.14% DM) was the highest. In general, better silage quality and a higher total yield were obtained when soybean was sown at the planting density of 150,000 plants ha−1 after 9 days of corn sowing. [ABSTRACT FROM AUTHOR]

Intercropping improves land-use efficiency under conditions of limited land and resources, but no information is currently available pertaining to land-use efficiency and silage quality based on whole-plant utilization. Therefore, a two-year field experiment was conducted with the following conditions: three maize–soybean strip intercropping patterns (SIPs), comprising two maize rows along with two, three, or four soybean rows (2M2S, 2M3S, and 2M4S, respectively); and two sole cropping patterns of maize (SM) and soybean (SS). The aim was to evaluate the biomass yield and silage quality under each condition. Our results showed that all SIPs had a land equivalent ratio (LER) of over 1.6 based on both fresh and dry matter yield, and a higher whole plant yield, compared to sole cropping. Specifically, 2M3S exhibited the highest whole crop dry matter LER (1.8–1.9) and yield (24.6–27.2 t ha−1) compared to SM and SS (20.88–21.49 and 3.48–4.79 t ha−1, respectively). Maize–soybean mixed silages also showed better fermentation quality with higher lactic acid content (1–3%) and lower ammonia-N content (2–8%) compared to SS silages, and higher crude protein content (1–1.5%) with lower ammonia-N content (1–2%) compared to SM silage. Among the intercropping patterns, 2M3S had the highest fermentation quality index V-score (92–95). Consequently, maize–soybean strip intercropping improved silage quality and biomass yield, with 2M3S being recommended, due to its highest LER and biomass yield, and most optimal silage quality. [ABSTRACT FROM AUTHOR]

The In1−xGaxAsyP1−y submicron wires with adjustable wavelengths directly grown by metalorganic chemical vapor deposition on a V-groove-patterned Si (001) substrate are reported in this paper. To ensure the material quality, aspect ratio trapping and selective area growth methods are used. By changing the parameters in the epitaxy process, we realize the adjustment of the material energy band of In1−xGaxAsyP1−y submicron wires. By further optimizing the growth conditions, we realize high-quality submicron wires. The morphology of the submicron wires is characterized by scanning electron microscopy and transmission electron microscopy. Through high-resolution X-ray diffraction measurement, it is disclosed that the lattice of the optimized In1−xGaxAsyP1−y part matches that of InP. A PL spectrum test shows that the PL spectrum peak is from 1260 nm to 1340 nm. The In1−xGaxAsyP1−y can be used as a well material or barrier material in a quantum well, which would promote the development of silicon-based lasers. [ABSTRACT FROM AUTHOR]

The dynamic of soil-borne disease is closely related to the rhizosphere microbial communities. Maize–soybean relay strip intercropping has been shown to significantly control the type of soybean root rot that tends to occur in monoculture. However, it is still unknown whether the rhizosphere microbial community participates in the regulation of intercropped soybean root rot. In this study, rhizosphere Fusarium and Trichoderma communities were compared in either healthy or root-rotted rhizosphere soil from monocultured and intercropped soybean, and our results showed the abundance of rhizosphere Fusarium in intercropping was remarkably different from monoculture. Of four species identified, F. oxysporum was the most aggressive and more frequently isolated in diseased soil of monoculture. In contrast, Trichoderma was largely accumulated in healthy rhizosphere soil of intercropping rather than monoculture. T. harzianum dramatically increased in the rhizosphere of intercropping, while T. virens and T. afroharzianum also exhibited distinct isolation frequency. For the antagonism test in vitro, Trichoderma strains had antagonistic effects on F. oxysporum with the percentage of mycelial inhibition ranging from 50.59–92.94%, and they displayed good mycoparasitic abilities against F. oxysporum through coiling around and entering into the hyphae, expanding along the cell–cell lumen and even dissolving cell walls of the target fungus. These results indicate maize–soybean relay strip intercropping significantly increases the density and composition proportion of beneficial Trichoderma to antagonize the pathogenic Fusarium species in rhizosphere, thus potentially contributing to the suppression of soybean root rot under the intercropping. [ABSTRACT FROM AUTHOR]

The frequency of heavy rains is increasing with climate change in regions that already have high annual rainfall (i.e., Sichuan, China). Crop response under such high-rainfall conditions is to increase dry matter investment in vegetative parts rather than reproductive parts. In the case of soybean, leaf redundancy prevails, which reduces the light transmittance and seed yield. However, moderate defoliation of soybean canopy could reduce leaf redundancy and improve soybean yield, especially under high-rainfall conditions. Therefore, the effects of three defoliation treatments (T1, 15%; T2, 30%; and T3, 45% defoliation from the top of the soybean canopy; defoliation treatments were applied at the pod initiation stage of soybean) on the growth and yield parameters of soybean were evaluated through field experiments in the summer of 2017, 2018, and 2019. All results were compared with nondefoliated soybean plants (CK) under high-rainfall conditions. Compared with CK, treatment T1 significantly (p < 0. 05) improved the light transmittance and photosynthetic rate of soybean. Consequently, the leaf greenness was enhanced by 22%, which delayed the leaf senescence by 13% at physiological maturity. Besides, compared to CK, soybean plants achieved the highest values of crop growth rate in T1, which increased the total dry matter accumulation (by 6%) and its translocation to vegetative parts (by 4%) and reproductive parts (by 8%) at physiological maturity. This improved soybean growth and dry matter partitioning to reproductive parts in T1 enhanced the pod number (by 23%, from 823.8 m−2 in CK to 1012.7 m−2 in T1) and seed number (by 11%, from 1181.4 m−2 in CK to 1311.7 m−2 in T1), whereas the heavy defoliation treatments considerably decreased all measured growth and yield parameters. On average, treatment T1 increased soybean seed yield by 9% (from 2120.2 kg ha−1 in CK to 2318.2 kg ha−1 in T1), while T2 and T3 decreased soybean seed yield by 19% and 33%, respectively, compared to CK. Overall, these findings indicate that the optimum defoliation, i.e., T1 (15% defoliation), can decrease leaf redundancy and increase seed yield by reducing the adverse effects of mutual shading and increasing the dry matter translocation to reproductive parts than vegetative parts in soybean, especially under high-rainfall conditions. Future studies are needed to understand the internal signaling and the molecular mechanism controlling and regulating dry matter production and partitioning in soybean, especially from the pod initiation stage to the physiological maturity stage. [ABSTRACT FROM AUTHOR]

Bats are natural reservoirs for many viruses, including several that are zoonotic. Two unusual H17N10 and H18N11 influenza viruses have been found in New World bats. Although neither of these viruses have been isolated, infectious clone technology has permitted significant progress to understand their biology, which include unique features compared to all other known influenza A viruses. In addition, an H9N2-like influenza A virus was isolated from Old World bats and it shows similar characteristics of normal influenza A viruses. In this review, current status and perspective on influenza A viruses identified in bats is reviewed and discussed. [ABSTRACT FROM AUTHOR]

To lighten and stiffen the structural components used in automobile bodies, the variable curvature local-induction-heating bending forming (VC-LIHBF) technology was developed. However, few studies have been conducted on the optimization of the structural and geometric parameters of the inductor and the parameters of the LIHBF process. This paper focuses on the improvement of the cross-sectional distortion and the forming limit of the thin-walled rectangular steel tubes (TWRSTs) without the mandrel support. In this work, a coupled thermo-mechanical finite element analysis (FEA) method is developed to investigate the structural and geometric parameters of the inductor and the deformation behavior during VC-LIHBF. The temperature distribution is calculated by the electromagnetic and heat transfer analysis, and the stress distribution is obtained by the deformation analysis. Experiments were carried out to validate the reliability of the proposed finite element model (FEM). The results indicate that the forming quality of the TWRST is significantly affected by the structural and geometric parameters of the inductor, the feed rate of the tube, the push speed of the bending roller and the distance between the inductor and the bending roller. The appropriate inductor and process parameters for a B1500HS TWRST were determined. The findings of this study may provide important guidance for practical manufacturing via the LIHBF process. [ABSTRACT FROM AUTHOR]

Lodging is one of the most chronic restraints of the maize-soybean intercropping system, which causes a serious threat to agriculture development and sustainability. In the maize-soybean intercropping system, shade is a major causative agent that is triggered by the higher stem length of a maize plant. Many morphological and anatomical characteristics are involved in the lodging phenomenon, along with the chemical configuration of the stem. Due to maize shading, soybean stem evolves the shade avoidance response and resulting in the stem elongation that leads to severe lodging stress. However, the major agro-techniques that are required to explore the lodging stress in the maize-soybean intercropping system for sustainable agriculture have not been precisely elucidated yet. Therefore, the present review is tempted to compare the conceptual insights with preceding published researches and proposed the important techniques which could be applied to overcome the devastating effects of lodging. We further explored that, lodging stress management is dependent on multiple approaches such as agronomical, chemical and genetics which could be helpful to reduce the lodging threats in the maize-soybean intercropping system. Nonetheless, many queries needed to explicate the complex phenomenon of lodging. Henceforth, the agronomists, physiologists, molecular actors and breeders require further exploration to fix this challenging problem. [ABSTRACT FROM AUTHOR]

In this study, soybean root distribution in an inter-cropping system was influenced by various environmental and biotic cues. However, it is still unknown how root development and distribution in inter-cropping responds to aboveground light conditions. Herein, soybeans were inter- and monocropped with P (phosphorus) treatments of 0 and 20 kg P ha yr−1 (P0 and P20, respectively) in field experiment over 4 years. In 2019, a pot experiment was conducted as the supplement to the field experiment. Shade from sowing to V5 (Five trifoliolates unroll) and light (SL) was used to imitate the light condition of soybeans in a relay trip inter-cropping system, while light then shade from V5 to maturity (LS) was used to imitate the light condition of soybeans when monocropped. Compared to monocropping, P uptake and root distribution in the upper 0–15 cm soil layer increased when inter-cropped. Inter-cropped soybeans suffered serious shade by maize during a common-growth period, which resulted in the inhibition of primary root growth and a modified auxin synthesis center and response. During the solo-existing period, plant photosynthetic capacity and sucrose accumulation increased under ameliorated light in SL (shade-light). Increased light during the reproductive stage significantly decreased leaf P concentration in SL under both P-sufficient and P-deficient conditions. Transcripts of a P starvation response gene (GmPHR25) in leaves and genes (GmEXPB2) involved in root growth were upregulated by ameliorated light during the reproductive stage. Furthermore, during the reproductive stage, more light interception increased the auxin concentration and expression of GmYUCCA14 (encoding the auxin synthesis) and GmTIR1C (auxin receptor) in roots. Across the field and pot experiments, increased lateral root growth and shallower root distribution were associated with inhibited primary root growth during the seedling stage and ameliorated light conditions in the reproductive stage. Consequently, this improved topsoil foraging and P uptake of inter-cropped soybeans. It is suggested that the various light conditions (shade-light) mediating leaf P status and sucrose transport can regulate auxin synthesis and respond to root formation and distribution. [ABSTRACT FROM AUTHOR]

The border row crop in strip intercropped maize is often exposed to heterogeneous light conditions, resulting in increased photosynthesis and yield decreased. Previous studies have focused on photosynthetic productivity, whereas carbon allocation could also be one of the major causes of decreased yield. However, carbon distribution remains unclear in partially shaded conditions. In the present study, we applied heterogeneous light conditions (T), and one side of plants was shaded (T-30%), keeping the other side fully exposed to light (T-100%), as compared to control plants that were exposed entirely to full-light (CK). Dry weight, carbon assimilation, 13C abundance, and transport tissue structure were analyzed to clarify the carbon distribution in partial shading of plants. T caused a marked decline in dry weight and harvest index (HI), whereas dry weight in unshaded and shaded leaves did not differ. Net photosynthesis rate (Pn), the activity of sucrose phosphate synthase enzymes (SPS), and sucrose concentration increased in unshaded leaves. Appropriately, 5.7% of the 13C from unshaded leaves was transferred to shaded leaves. Furthermore, plasmodesma density in the unshaded (T-100%) and shaded (T-30%) leaves in T was not significantly different but was lower than that of CK. Similarly, the vascular bundle total area of T was decreased. 13C transfer from unshaded leaves to ear in T was decreased by 18.0% compared with that in CK. Moreover, 13C and sucrose concentration of stem in T were higher than those in CK. Our results suggested that, under heterogeneous light, shaded leaves as a sink imported the carbohydrates from the unshaded leaves. Ear and shaded leaf competed for carbohydrates, and were not conducive to tissue structure of sucrose transport, resulting in a decrease in the carbon proportion in the ear, harvest index, and ear weight. [ABSTRACT FROM AUTHOR]

Seed germination is one of the most important stages during plant life cycle, and DOG1 (Delay of germination1) plays a pivotal regulatory role in seed dormancy and germination. In this study, we have identified the DOG1-Like (DOG1L) family in soybean (Glycine max), a staple oil crop worldwide, and investigated their chromosomal distribution, structure and expression patterns. The results showed that the GmDOG1L family is composed of 40 members, which can be divided into six subgroups, according to their evolutionary relationship with other known DOG1-Like genes. These GmDOG1Ls are distributed on 18 of 20 chromosomes in the soybean genome and the number of exons for all the 40 GmDOG1Ls varied greatly. Members of the different subgroups possess a similar motif structure composition. qRT-PCR assay showed that the expression patterns of different GmDOG1Ls were significantly altered in various tissues, and some GmDOG1Ls expressed primarily in soybean seeds. Gibberellic acid (GA) remarkably inhibited the expression of most of GmDOG1Ls, whereas Abscisic acid (ABA) inhibited some of the GmDOG1Ls expression while promoting others. It is speculated that some GmDOG1Ls regulate seed dormancy and germination by directly or indirectly relating to ABA and GA pathways, with complex interaction networks. This study provides an important theoretical basis for further investigation about the regulatory roles of GmDOG1L family on soybean seed germination. [ABSTRACT FROM AUTHOR]

The shading of maize and self-shading are the key factors affecting the stem lignin biosynthesis and lodging resistance of soybean at middle and later growth stages in the strip intercropping system. A study was designed to explore the regulation mechanism of lignin metabolism and different planting densities; PD1, PD2, and PD3 were used having a total number of 17 plants m−2, 20 plants m−2, and 25 plants m−2, respectively, on the lodging resistance of strip intercropped soybean stem. Our results depicted that the lower planting density (PD1) appropriately promoted the leaf photosynthesis activities (Pn), increase the activity of lignin-related enzymes and the accumulation of carbohydrates in stems, and eventually enhanced the lodging resistance of the strip intercropped soybean stem. Correlation analysis also showed that the lodging resistance index of soybean stem was significantly correlated with the available light for soybean canopy and Pn strip intercropped soybean stem characteristics and activities of enzymes related to lignin synthesis among the different planting densities. The findings of our research will be useful in future studies to understand the relationship between different light environment, planting densities, and lodging resistance of intercropped soybean and also guide the optimum planting density in maize–soybean intercropping system. [ABSTRACT FROM AUTHOR]

Water resources planning and management depend on the quality of climatic data, particularly rainfall data, for reliable hydrological modeling. This can be very problematic in transboundary rivers with limited disclosing of data among the riparian countries. Satellite precipitation products are recognized as a promising source to substitute the ground-based observations in these conditions. This research aims to assess the feasibility of using a satellite-based precipitation product for better hydrological modeling in an ungauged and riparian river in Pakistan, i.e., the Chenab River. A semidistributed hydrological model of The soil and water assessment tool (SWAT) was set up and two renowned satellite precipitation products, i.e., global precipitation mission (GPM) IMERG-F v6 and tropical rainfall measuring mission (TRMM) 3B42 v7, were selected to assess the runoff pattern in Chenab River. The calibration was done from 2001–2006 with two years of a warmup period. The validation (2007–2010) results exhibit higher correlation between observed and simulated discharges at monthly timescale simulations, IMERG-F (R2 = 0.89, NSE = 0.82), 3B42 (R2 = 0.85, NSE = 0.72), rather than daily timescale simulations, IMERG-F (R2 = 0.66, NSE = 0.61), 3B42 (R2 = 0.64, NSE = 0.54). Moreover, the comparison between IMERG-F and 3B42, shows that IMERG-F is superior to 3B42 by indicating higher R2, NSE and lower percent bias (PBIAS) at both monthly and daily timescale. The results are strengthened by Taylor diagram statistics, which represent a higher correlation (R) and less RMS error between observed and simulated values for IMERG-F. IMERG-F has great potential utility in the Chenab River catchment as it outperformed the 3B42 precipitation in this study. However, its poor skill of capturing peaks at daily timescale remains, leaving a room for IMERG-F to improve its algorithm in the upcoming release. [ABSTRACT FROM AUTHOR]

Maize/soybean relay strip intercropping has been widely practiced in Southwest China due to its high productivity and effective application of agricultural resources; however, several seedborne diseases such as seedling blight, pod and seed decay are frequently observed causing severe yield loss and low seed quality. So far, the population and pathogenicity of the seedborne fungi associated with intercropped soybean remain unexplored. In this study, seeds of 12 soybean cultivars screened for intercropping were collected from three growing regions in Sichuan Province of Southwest China, and the seedborne fungi were isolated from the surface-sterilized seeds. Based on sequence analysis of ribosomal DNA internal transcribed spacer (rDNA ITS), 148 isolates were identified into 13 fungal genera, among which Fusarium covered 55.0% as the biggest population followed by Colletotrichum. Furthermore, Fusarium isolates were classified into five distinct species comprising F. fujikuroi, F. proliferatum, F. verticillioides, F. asiaticum and F. incarnatum through sequence analysis of translation elongation factor 1 alpha (EF-1α) and DNA-directed RNA ploymerase II second largest subunit (RPB2). Among them, F. fujikuroi accounted for 51.22% (42/82) and was isolated from 91.7% (11/12) soybean varieties. Pathogenicity assay showed that five Fusarium species were able to infect the seeds of soybean cultivar "Nandou12" and caused water-soaked or rot symptoms, while F. fujikuroi and F. asiaticum had much higher aggressiveness than other species with significant reductions of seed fresh weight and germination percentage. Accordingly, this study indicates that Fusarium species are the dominant seedborne fungi in the intercropped soybean in Sichuan, China, and this provides some useful references for the effective management of seedborne fungal diseases as well as soybean resistance breeding in maize/soybean relay strip intercropping. [ABSTRACT FROM AUTHOR]

Intercropping is an intensive agricultural cropping system widely practiced for enhanced yield and nutrient acquisition advantages. A two-year maize–soybean intercropping (MSI) field study was performed in 2018 and 2019 to assess the effects of potassium (K) fertilizer application on biomass accumulation and distribution of essential nutrients in the various plant parts (root, green biomass and seed) of maize–soybean intercropping (MSI). Three different treatments of K fertilizer applications (T0: no potassium application; T1: maize 40, soybeans 30 and T2: maize 80, soybeans 60 kg ha−1) were designed with 2 rows of maize by wide, narrow row planting in row arrangements of 160 cm + 40 cm. Soybeans were grown in 2 wide rows at a width of 40 cm and a row spacing of 60 cm between the rows of maize and soybeans, while the sole maize (SM) and sole soybean (SS) were grown with 70-cm and 50-cm row spacing, respectively. The results of the two-year study confirmed that, as compared to T0, T2 significantly increased nitrogen, phosphate and potassium (NPK) accumulation in all maize parts by 27%, 16% and 20% grain, 23%, 22% and 14% green biomass and 30%, 17% and 15% root, respectively. In soybean treatments, T2 significantly increased NPK accumulation by 23%, 22% and 24% grain, 16%, 15% and 12% green biomass and 18%, 19% and 20% root, respectively. The increased accumulation of nutrients under T2 raised the overall biomass and its distribution to root, green biomass and grain in maize and soybeans by 11% and 18% and 16% and 19%, 20% and 12%, respectively, compared to T0. On average, after two years of experiments, the T2 intercropped maize and the soybeans showed 103% and 64% of the sole yield and attained the maximum LER of 1.66 and 1.68, respectively. Our results reveal that managing optimum K level application (80:60 kg ha−1) can accelerate biomass accumulation and distribution of other essential nutrients in the plant parts of intercropped maize and soybeans. Therefore, it is immensely important to concern potassium application levels in developing a sustainable maize–soybean intercropping systems for achieving higher productivity and land equivalent ratio (LER). [ABSTRACT FROM AUTHOR]

Shade is widespread in agricultural production and affects lignin biosynthesis and lodging resistance of crops. We explored the effects of shade intensity on lignin biosynthesis and lodging resistance at the physiological and molecular levels in two soybean cultivars (Nandou12 and E93) with different shade tolerance under four progressively severe shade treatments, S0–S3 (S0: no shade, S1: slight shade, S2: moderate shade, S3: heavy shade). Our results showed no significant difference in breaking strength of the two cultivars under S1 and S0 treatments, with no prominent decrease in the lodging resistance index. The activity of lignin biosynthesis rate-limiting enzymes phenylalanine ammonia-lyase (PAL), peroxidase and cinnamyl alcohol dehydrogenase (CAD), which were considerably related to the two lodging resistance indexes above, was not significantly decreased by slight shade, while 4-coumaric acid ligase (4CL) activity was increased. Most genes involved in lignin biosynthesis were not significantly down-regulated by slight shade (S1) compared to S0, while p-coumarate 3-hydroxylase (C3H), 4-coumaric acid ligase (4CL) and laccase (LAC) genes were upregulated. Under heavy shade (S3), enzyme activity and gene expression associated with lignin synthesis in both soybean cultivars were strongly inhibited; moreover, stem mechanical strength and lodging resistance were remarkably decreased compared with those under S0. These physiological and molecular changes suggested that applicable shade levels do not significantly affect the mechanical strength and lodging resistance of soybean stem. Exploiting the lodging resistance potential of existing soybean cultivars was an effective and efficient way to address yield reduction caused by lodging in intercropped soybeans. [ABSTRACT FROM AUTHOR]

Fusarium species are the most detrimental pathogens of soybean root rot worldwide, causing large loss in soybean production. Maize/soybean relay strip intercropping has significant advantages on the increase of crop yields and efficient use of agricultural resources, but its effects on the occurrence and pathogen population of soybean root rot are rarely known. In this study, root rot was investigated in the fields of the continuous maize/soybean strip relay intercropping and soybean monoculture. Fusarium species were isolated from diseased soybean roots and identified based on sequence analysis of translation elongation factor 1α (EF-1α) and RNA polymerase II second largest subunit (RPB2), and the diversity and pathogenicity of these species were also analyzed. Our results showed that intercropping significantly decreased soybean root rot over monoculture. A more diverse Fusarium population including Fusarium solani species complex (FSSC), F. incarnatum-equiseti species complex (FIESC), F. oxysporum, F. fujikuroi, F. proliferatum and F. verticillioides, F. graminearum and F. asiaticum was identified from intercropping while FSSC, FIESC, F. oxysporum, F. commune, F. asiaticum and F. meridionale were found from monoculture. All Fusarium species caused soybean root infection but exhibited distinct aggressiveness. The most aggressive F. oxysporum was more frequently isolated in monoculture than intercropping. FSSC and FIESC were the dominant species complex and differed in their aggressiveness. Additionally, F. fujikuroi, F. proliferatum and F. verticillioides were specifically identified from intercropping with weak or middle aggressiveness. Except for F. graminearum, F. meridionale and F. asiaticum were firstly reported to cause soybean root rot in China. This study indicates maize/soybean relay strip intercropping can reduce soybean root rot, change the diversity and aggressiveness of Fusarium species, which provides an important reference for effective management of this disease. [ABSTRACT FROM AUTHOR]

Shading created by intercropping reduces the photosynthetic capacity of soybean plants but also directly affects the pod setting process of soybean. However, which of the changed aspects induce the yield differences in intercropped soybean cultivars is still unknown. Four soybean cultivars with similar yield and growth and development processes in monoculture were selected by a pre-experiment. Field experiments were carried out from 2015 to 2017 to investigate the leaf photosynthetic parameters, total biomass, reproductive characteristics, yield and yield components of soybean. The yield of soybean cultivars was significantly decreased in intercropping systems and the yield of cultivars (cvs.) ZH39 and QH34 were considerably higher than those of cvs. HD19 and HD20. Besides, the pod and seed number and harvest index were also reduced by intercropping and the yield components of cvs. ZH39 and QH34 in intercropping were significantly higher than those of cvs. HD19 and HD20, other than the seed size. Although the parameters of leaf photosynthetic capacity (leaf area index, net photosynthetic rate, and chlorophyll content) of soybean were changed by intercropping, there was no significant difference among cultivars. Additionally, the CGRR1–R5 (crop growth rate between R1 and R5) of intercropped soybean was lower than that of monoculture, while no significant differences were observed in different cultivars. The reproductive biomass at R5 was significantly different among soybean cultivars, and the reproductive partitioning and seed set efficiency of different cultivars were varied by the reproductive biomass at R5. Therefore, high-yielding cultivars in intercropping can achieve higher yield due to more reproductive structures survived at R5. [ABSTRACT FROM AUTHOR]

The effect of soybean soluble polysaccharide (SSPS) on the formation of glucono-δ-lactone (GDL)-induced soybean protein isolate (SPI) gel was investigated. Electrophoretic analysis showed the SSPS did not change the electrophoretic behavior of SPI during the formation of SPI gel. However, infrared analysis indicated the β-sheet content increased, and the contents of random coil and α-helix decreased in both cooked SPI and SPI gel. The SSPS and SPI might conjugate via the Maillard reaction according to the results of grafting degree, color change, and infrared analyses. The main interactions during the formation of SPI gel changed from non-covalent to electrostatic interaction after adding SSPS. Sulfhydryl group content also increased in both cooked SPI and SPI gel. The water-holding capacity and gel strength of SPI gel decreased as the SSPS concentration increased. Larger aggregate holes were observed in the microstructure of SPI gel at higher SSPS concentration. Thus, SSPS could covalently conjugate with SPI and influence the formation of hydrogen bonds, disulfide bonds, and electrostatic interaction among SPI molecules to eventually form a loose gel network. [ABSTRACT FROM AUTHOR]

Intercropping has been considered as a kind of a sustainable agricultural cropping system. In southwest China, maize/soybean strip intercropping has commonly been practised under local limited agricultural land resources. However, heavy rainfall in combination with high humidity and low temperatures cause severe pod and seed deterioration in the maturity and pre-harvesting stages of intercropped soybean. Numerous Fusarium species have been reported as the dominant pathogens of soybean root rot, seedling blight, as well as pod field mold in this area. However, the diversity and pathogenicity of Fusarium species on soybean pods remain unclear. In the current study, diseased soybean pods were collected during the cropping season of 2018 from five different intercropped soybean producing areas. A total of 83 Fusarium isolates were isolated and identified as F. fujikuroi, F. graminearum, F. proliferatum, and F. incarnatum-equiseti species complex based on morphological characteristics and phylogenetic analysis of the nucleotide sequence of EF1-α and RPB2 genes. Pathogenicity tests demonstrated that all Fusarium species were pathogenic to seeds of the intercropped soybean cultivar Nandou12. Fusarium fujikuroi had the maximum disease severity, with a significant reduction of seed germination rate, root length, and seed weight, followed by F. equiseti, F. graminearum, F. proliferatum, and F. incarnatum. Additionally, the diversity of Fusarium species on soybean pods was also considerably distinct according to the geographical origin and soybean varieties. Thus, the findings of the current study will be helpful for the management and resistance breeding of soybean pod decay in the maize/soybean intercropping system. [ABSTRACT FROM AUTHOR]

Shading in the intercropping system is a major abiotic factor which influences soybean growth and development, while soybean mosaic virus (SMV) is a biotic factor that limits the yield and quality of soybean. However, little is known about the defense response of soybean to SMV in the shade. Thus, in the current study, both intensity and quality (red:far-red, R:FR) of the light were changed to simulate the shaded environment and comparative transcriptome analysis was performed. Morphologically, plant growth was inhibited by SMV, which decreased 35.93% of plant height and 8.97% of stem diameter in the shade. A total of 3548 and 4319 differentially expressed genes (DEGs) were identified in soybean plants infected with SMV under normal light and in the shade. Enrichment analysis showed that the plant defense-related genes were upregulated under normal light but downregulated in the shade. Pathways that were repressed include plant-pathogen interaction, secondary metabolism, sugar metabolism, and vitamin metabolism. In addition, genes associated with signaling pathways such as salicylic acid (SA), jasmonic acid (JA), and ethylene (ETH) were also downregulated in the shade. A qRT-PCR assay of 15 DEGs was performed to confirm transcriptome results. According to our knowledge, this is the first report on soybean response to dual stress factors. These results provide insights into the molecular mechanisms in which soybean plants were infected with SMV in the shade. [ABSTRACT FROM AUTHOR]