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Your search keyword '"Yamanaka, Shigeki"' showing total 5 results
Start Over Author "Yamanaka, Shigeki" Publisher mdpi
5 results on '"Yamanaka, Shigeki"'

1. bmp-2 Gene-Transferred Skeletal Muscles with Needle-Type Electrodes as Efficient and Reliable Biomaterials for Bone Regeneration.

Author

Kawai, Mariko Yamamoto, Yoshida, Takeshi, Kato, Tomoki, Watanabe, Takuma, Kashiwagi, Marina, Yamanaka, Shigeki, Yamamoto, Hiromitsu, Nagahiro, Shigeki, Iwamoto, Tsutomu, Masud, Khan, Aoki, Kazuhiro, Ohura, Kiyoshi, and Nakao, Kazumasa

Subjects
SKELETAL muscle, BIOMATERIALS, ELECTRODES, SOFT X rays, GENETIC transformation, BONE regeneration, GENETIC vectors
Abstract

Background: Bone morphogenetic protein-2 (bmp-2) has a high potential to induce bone tissue formation in skeletal muscles. We developed a bone induction system in skeletal muscles using the bmp-2 gene through in vivo electroporation. Natural bone tissues with skeletal muscles can be considered potential candidates for biomaterials. However, our previous system using plate-type electrodes did not achieve a 100% success rate in inducing bone tissues in skeletal muscles. In this study, we aimed to enhance the efficiency of bone tissue formation in skeletal muscles by using a non-viral bmp-2 gene expression plasmid vector (pCAGGS-bmp-2) and needle-type electrodes. Methods: We injected the bmp-2 gene with pCAGGS-bmp-2 into the skeletal muscles of rats' legs and immediately placed needle-type electrodes there. Skeletal tissues were then observed on the 21st day after gene transfer using soft X-ray and histological analyses. Results: The use of needle-type electrodes resulted in a 100% success rate in inducing bone tissues in skeletal muscles. In contrast, the plate-type electrodes only exhibited a 33% success rate. Thus, needle-type electrodes can be more efficient and reliable for transferring the bmp-2 gene to skeletal muscles, making them potential biomaterials for repairing bone defects. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

Author

1000040379839, Kawai, Mariko, 1000080845347, Ozasa, Ryosuke, 1000050508835, Ishimoto, Takuya, 1000030243182, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, 1000020131378, Ohura, Kiyoshi, 1000040379839, Kawai, Mariko, 1000080845347, Ozasa, Ryosuke, 1000050508835, Ishimoto, Takuya, 1000030243182, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, 1000020131378, and Ohura, Kiyoshi

Abstract

Kawai M.Y., Ozasa R., Ishimoto T., et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15, 3, 993. https://doi.org/10.3390/ma15030993., The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.

Published
2022

3. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer.

Author

Kawai, Mariko Yamamoto, Ozasa, Ryosuke, Ishimoto, Takuya, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, and Ohura, Kiyoshi

Subjects
REGENERATIVE medicine, BIOMATERIALS, GUIDED tissue regeneration, MESENCHYMAL stem cell differentiation, REGENERATION (Biology)
Abstract

The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
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4. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

Author

Kawai, Mariko, Ozasa, Ryosuke, Ishimoto, Takuya, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, Ohura, Kiyoshi, Kawai, Mariko, Ozasa, Ryosuke, Ishimoto, Takuya, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, and Ohura, Kiyoshi

Abstract

Kawai M.Y., Ozasa R., Ishimoto T., et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15, 3, 993. https://doi.org/10.3390/ma15030993., The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.

5. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer

Author

Kawai, Mariko, Ozasa, Ryosuke, Ishimoto, Takuya, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, Ohura, Kiyoshi, Kawai, Mariko, Ozasa, Ryosuke, Ishimoto, Takuya, Nakano, Takayoshi, Yamamoto, Hiromitsu, Kashiwagi, Marina, Yamanaka, Shigeki, Nakao, Kazumasa, Maruyama, Hiroki, Bessho, Kazuhisa, and Ohura, Kiyoshi

Abstract

Kawai M.Y., Ozasa R., Ishimoto T., et al. Periodontal Tissue as a Biomaterial for Hard-Tissue Regeneration following bmp-2 Gene Transfer. Materials, 15, 3, 993. https://doi.org/10.3390/ma15030993., The application of periodontal tissue in regenerative medicine has gained increasing interest since it has a high potential to induce hard-tissue regeneration, and is easy to handle and graft to other areas of the oral cavity or tissues. Additionally, bone morphogenetic protein-2 (BMP-2) has a high potential to induce the differentiation of mesenchymal stem cells into osteogenic cells. We previously developed a system for a gene transfer to the periodontal tissues in animal models. In this study, we aimed to reveal the potential and efficiency of periodontal tissue as a biomaterial for hard-tissue regeneration following a bmp-2 gene transfer. A non-viral expression vector carrying bmp-2 was injected into the palate of the periodontal tissues of Wistar rats, followed by electroporation. The periodontal tissues were analyzed through bone morphometric analyses, including mineral apposition rate (MAR) determination and collagen micro-arrangement, which is a bone quality parameter, before and after a gene transfer. The MAR was significantly higher 3–6 d after the gene transfer than that before the gene transfer. Collagen orientation was normally maintained even after the bmp-2 gene transfer, suggesting that the bmp-2 gene transfer has no adverse effects on bone quality. Our results suggest that periodontal tissue electroporated with bmp-2 could be a novel biomaterial candidate for hard-tissue regeneration therapy.

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