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Cardiovascular diseases (CVDs) are the leading cause of death globally, with the majority of deaths occurring in low- and middle-income countries. Understanding the molecular aspects of CVDs and developing cost-effective programs and policies to control cardiovascular risk factors is crucial. This special issue focuses on various risk factors for CVDs, including modifiable factors like unhealthy diet and physical inactivity, as well as non-modifiable factors like genetic variations. The articles in this issue explore molecular targets, biomarkers, and signaling pathways related to cardiovascular risk factors. Topics covered include calcium channels, vascular inflammation and calcification, smoking and cannabis use, the renin-angiotensin-aldosterone system, sphingosine-1-phosphate signaling, epigenetic modifications, hypoglycemia, peripartum cardiomyopathy, chronic kidney disease, and kidney function monitoring. While not all risk factors are covered, this special issue aims to contribute to our understanding of cardiovascular risk factors and strategies for prevention. [Extracted from the article]

An elevated level of circulating homocysteine (Hcy) has been regarded as an independent risk factor for cardiovascular disease; however, the clinical benefit of Hcy lowering-therapy is not satisfying. To explore potential unrevealed mechanisms, we investigated the roles of Ca2+ influx through TRPC channels and regulation by Hcy–copper complexes. Using primary cultured human aortic endothelial cells and HEK-293 T-REx cells with inducible TRPC gene expression, we found that Hcy increased the Ca2+ influx in vascular endothelial cells through the activation of TRPC4 and TRPC5. The activity of TRPC4 and TRPC5 was regulated by extracellular divalent copper (Cu2+) and Hcy. Hcy prevented channel activation by divalent copper, but monovalent copper (Cu+) had no effect on the TRPC channels. The glutamic acids (E542/E543) and the cysteine residue (C554) in the extracellular pore region of the TRPC4 channel mediated the effect of Hcy–copper complexes. The interaction of Hcy–copper significantly regulated endothelial proliferation, migration, and angiogenesis. Our results suggest that Hcy–copper complexes function as a new pair of endogenous regulators for TRPC channel activity. This finding gives a new understanding of the pathogenesis of hyperhomocysteinemia and may explain the unsatisfying clinical outcome of Hcy-lowering therapy and the potential benefit of copper-chelating therapy. [ABSTRACT FROM AUTHOR]

Laminated shales are widely developed in the Dongying Sag and have attracted much attention as an oil reservoir. Macroscopically, these shales generally have multi-scale cyclicity, which is closely related to the development of laminae. Therefore, analyzing the origin of their cyclicity is helpful to understanding the formation mechanism of laminated shales and the vertical heterogeneity of shale reservoirs, which are of great significance for continental shale oil exploration and development. In this study, a gamma ray (GR) logging series, high-resolution elemental geochemical data, high-resolution core scanning photos and grayscale data, and mineralogical data were used to characterize the cyclicity of shale at different scales, and their relationship with different astronomical cycles was discussed. The results show that the Es3L and Es4U shale in the Dongying Sag has cyclicity from the meter-scale to the ten-meter scale and then to the hundred-meter scale, which is mainly manifested by periodic changes in organic matter abundance, mineral composition, element abundance, and grayscale. These cycles of different scales coincide with different astronomical periods. Specifically, the hundred-meter scale cyclicity is mainly controlled by the very long orbital period; the ten-meter scale cyclicity is mainly related to the eccentricity cycle; while the precession period is the main driver of the meter-scale cyclicity. Finally, we propose a simplified model for illustrating the formation of rhythmic organic-rich shale. This study is helpful to understanding the origin of continental organic-rich shale and predicting shale reservoir properties. [ABSTRACT FROM AUTHOR]

Reservoir characteristics and the occurrence mechanism of shale gas outside of the Sichuan Basin are the research hotspots of normally pressured shales in China. Taking shales on the Anchang syncline from the periphery of the Sichuan Basin as an example, X-ray diffraction, organic geochemistry, and rock physical experiments were carried out to analyze the reservoir characteristics and their main geological controls on the normally pressured shales. The mineralogical results show that the studied shales from the Anchang syncline are mainly siliceous shales with a high quartz content (average of 57%). The quartz content of these normally pressured shales is of biological origin, as shown by the positive correlation between the quartz and organic carbon (TOC) contents. The average porosity of the studied shales is about 2.9%, which is lower than shales inside the Sichuan Basin. Organic matter pores are likely the primary storage space of the normally pressured shale gas, as shown by the positive relationship between the TOC content and porosity. However, scanning electron microscopy observations on the studied shales show that the pores in these normally pressured shales are poorly preserved; many pores have been subjected to compression and deformation due to tectonic movements. Compared to shales inside the Sichuan Basin, the effective thickness of shales outside of the Sichuan Basin is thin and the stratum dip is large. Thus, shale gas outside of the Sichuan Basin is apt to escape laterally along the bedding of the strata. After losing a significant amount of shale gas, the gas pressure decreases to normal pressure, which makes it difficult for the pores to resist compaction from the overlying strata. This is probably why most shale gas reservoirs outside of the Sichuan Basin are normally pressured, while the shale strata inside the Sichuan Basin are commonly overpressured. This study provides insights to understand the pore development and hydrocarbon occurrence on normally pressured shales outside of the Sichuan Basin. [ABSTRACT FROM AUTHOR]

The successful development of shale oil in China has claimed that laminated shale is a favorable lithofacies for the effective extraction of petroleum. Clarifying the role of laminae in shale oil generation, migration, storage, and enrichment is urgent and important. Starting from the describing and classifying of the lamina, the common methods and terms used to delineate lamina types are briefly summarized. The results of different schemes are often mutually inclusive, which prompted scholars to work towards a unified division scheme. The influencing factors of oil retention in shale systems, including organic matter (OM) type, total organic carbon (TOC) content, OM maturity, mineral composition, pore structure, and preservation conditions, are systematically discussed. Subsequently, comparative work on source rock quality, reservoir properties, and hydrocarbon expulsion efficiency of shales with different laminar structures is carried out. The comparison results of shale with different rock structures reveal that the laminated shale has a high expulsion efficiency. However, the strong oil generation capacity and superior storage space of laminated shale synergistically control the considerable amount of retained oil in the shale system. Especially the oil mobility of laminated shale is also considered because of great pore size and pore connectivity. The fine evaluation of laminar structure and prediction of laminar distribution has great significance for the selection of shale oil "sweet spot area" or "sweet spot interval". [ABSTRACT FROM AUTHOR]

The laminar structure of shale system has an important influence on the evaluation of hydrocarbon source rock quality, reservoir quality, and engineering quality, and it is receiving increasing attention. A systematic study of the lamina structure is not only of great scientific significance but also of vital practical importance for shale oil production. In this paper, the identification and description classification of shale laminae are first reviewed. Multiple scales and types indicate that a combination of different probe techniques is the basis for an accurate evaluation of shale laminar characteristics. The influence of laminae on shale reservoir, oil-bearing, mobility, and fracability properties is discussed systematically. A comparative analysis shows that shale systems with well-developed lamination facilitate the development of bedding fractures, thus improving the shale storage space. The average pore size and pore connectivity are also enhanced. These factors synergistically control the superior retention and flow capacity of shale oil in laminated shales. In such conditions, the high production of shale oil wells can still be achieved even if complex networks of fracturing cracks are difficult to form in shale systems with well-developed lamination. This work is helpful to reveal the enrichment mechanism of shale oil and clarify the high-yield law of hydrocarbons, so as to guide the selection of sweet spots. [ABSTRACT FROM AUTHOR]

Many sets of Paleozoic marine organic-rich shale strata have developed in South China. However, the exploration and development results of these shale formations are quite different. Based on the data of core experiment analysis, drilling, fracturing test of typical wells, the reservoir differences and controlling factors of four sets of typical marine organic-rich shale in southern China are investigated. The four sets of shale have obvious differences in reservoir characteristics. Ordovician–Silurian shale mainly develops siliceous shale, mixed shale and argillaceous shale, with large pore diameter, high porosity, moderate thermal maturity, large pore volume and specific surface area. Cambrian shale mainly develops siliceous shale and mixed shale, with small pore diameter, low porosity, high thermal maturity and smaller pore volume and specific surface area than Ordovician–Silurian shale. Devonian–Carboniferous shale has similar mineral composition to Ordovician–Silurian shale, with small pore diameter, low porosity, moderate thermal maturity and similar pore volume and specific surface area to that of Cambrian shale. Permian shale has very complex mineral composition, with large pore diameter, low to medium thermal maturity and small specific surface area. Mineral composition, thermal maturity and tectonic preservation conditions are the main factors controlling shale reservoir development. Siliceous minerals in Cambrian shale and Ordovician–Silurian shale are mainly of biological origin, which make the support capacity better than Devonian–Carboniferous shale and Permian shale (siliceous minerals are mainly of terrigenous origin and biological origin). Thermal maturity of Ordovician–Silurian shale and Devonian–Carboniferous shale is moderate, with a large number of organic pores developed. Thermal maturity of Cambrian shale and Permian shale is respectively too high and too low, the development of organic pores is significantly weaker than the two sets of shale above. There are obvious differences in tectonic preservation conditions inside and outside the Sichuan Basin. Shale reservoirs inside the Sichuan Basin are characterized by overpressure due to stable tectonic activities, while shale reservoirs outside the Sichuan Basin are generally normal–pressure. Four sets of marine shale in South China all have certain resource potentials, but the exploration and development of shale gas is still constrained by complicated geological conditions, single economic shale formation, high exploration and development costs and other aspects. It is necessary for further research on shale gas accumulation theory, exploration and development technology and related policies to promote the development of China's shale gas industry. [ABSTRACT FROM AUTHOR]

Organic-rich lacustrine shales are widely developed in China, and they have long been simply regarded as homogeneous source rocks, which restricts the understanding of intrasource oil accumulation. At present, the study of the LXF (Lower Member of the Xingouzui Formation) in the Jianghan Basin as an unconventional oil reservoir is still in its infancy, and the hydrocarbon accumulation mechanism is still unclear. Geochemical and mineralogical studies were carried out on a suite of samples from the 100-m-thick sequence, i.e., LXF II Oil Bed, by using XRD, SEM, MICP, and Rock-Eval pyrolysis. The results show that the II Oil Bed is rich in carbonate and poor in clay, so it shows a good fracturing tendency. The high degree of heterogeneity in mineral composition leads to frequent interbedding of different lithofacies. In the II Oil Bed, intercrystalline pores, interparticle pores, and intraparticle pores are developed, and micro-fractures are often observed. However, the main pore types, pore size distribution, and connectivity are quite different among lithofacies, and the carbonate-rich lithofacies have better reservoir capacity. The OM (organic matter) abundance of the II Oil Bed varies greatly and generally ranges from fair to very good. Coupled with its early-mature to mature Type II OM, it is considered to have the characteristics required for oil generation. Comprehensive analysis shows that the II Oil Bed has good shale oil exploration prospects, and the enrichment of shale oil in the sequence is the result of multiple factors matching. Firstly, high organic matter abundance is the material basis for shale oil enrichment. Secondly, thermal maturity is a prerequisite, and the difference in burial depth leads to the differential enrichment of shale oil in different areas. Thirdly, pores and micro-fractures developed in shale not only provide space for hydrocarbon storage, but also form a flow-path network. Finally, multi-scale intrasource migrations are key processes ranging from the scale of lithofacies to the intervals, which further results in the differential shale oil enrichment in different lithofacies and intervals. Considering the hydrocarbon generation capacity and reservoir quality, the prospective depth for shale oil exploration in the study area is >1350 m. The findings of this study can help in the better-understanding of the shale oil enrichment mechanism, and the optimization of future exploration strategies. [ABSTRACT FROM AUTHOR]

Objective: To detect the interactions between six functional polymorphisms in ABCA1 and obesity in Kazakhs with low HDL-C levels., Methods: A total of 204 patients with low HDL-C and 207 health control subjects, which were randomly selected from among 5692 adult Kazakhs, were matched for age and sex. We genotyped ABCA1 single nucleotide polymorphisms of rs2515602, rs3890182, rs2275542, rs2230806, rs1800976, and rs4149313., Results: (1) The genotypic and allelic frequencies of rs2515602, rs2230806 and rs4149313 were different between normal HDL-C and low HDL-C subjects, the genotypic frequency of rs2275542 was also different between normal HDL-C and low HDL-C subjects (p < 0.05); (2) the level of HDL-C (rs2515602 and rs2275542) in normal HDL-C subjects were different among the genotypes (p < 0.05); the levels of TC, LDL-C (rs2515602, rs4149313); TG (rs2515602, rs1800976, rs4149313) in low HDL-C patients were different among the genotypes (p < 0.05); (3) interactions between the rs3890182, rs2275542, rs180096, and rs4149313 polymorphisms in ABCA1 gene and obesity may be associated with low HDL-C disease; (4) the C-C-C-A-A-G, T-C-C-A-A-A, T-C-C-A-A-G, C-C-C-A-A-A, C-T-G-G-A-A, and T-T-C-G-A-A haplotypes were significant between the subjects with normal HDL-C and low HDL-C level (p < 0.05)., Conclusions: The differences in serum lipid levels between normal HDL-C and low HDL-C subjects among Kazakhs might partly result from ABCA1 gene polymorphisms; ABCA1 gene polymorphisms may be associated with low HDL-C disease; the low HDL-C disease might partly result from interactions between ABCA1 gene polymorphisms and obesity; the C-C-C-A-A-G, T-C-C-A-A-A, and T-C-C-A-A-G haplotypes may serve as risk factors of low HDL-C disease among Kazakhs, the C-C-C-A-A-A, C-T-G-G-A-A, and T-T-C-G-A-A haplotypes may serve as protective factor of low HDL-C disease among Kazakhs.