Search

Background: The impact of and countermeasures for Ureaplasma spp. in neonates remain controversial. The aim of this study was to evaluate the associated perinatal factors that can predict the likelihood of respiratory tract Ureaplasma spp. colonization and analyze the subsequent clinical course of affected infants, thereby providing the rationale for their diagnosis, treatment, and future study. Methods: This was a retrospective observational study of infants born at a gestational age (GA) of less than 32 weeks. Results: The prevalence of respiratory tract Ureaplasma spp. colonization was 25.8% (75/291), and it increased with a decrease in GA and birth weight (BW). Maternal vaginal Ureaplasma spp. colonization increased the risk of neonatal Ureaplasma spp. colonization, with an OR of 7.8 (95% CI: 3.1, 20.0). Infants with Ureaplasma spp. colonization had a higher white blood cell (WBC) count, normal C-reactive protein (CRP) level, and higher failure rate of weaning from mechanical ventilation (30.7% vs. 17.1%, p = 0.014); they also suffered more from interstitial pneumonia (20.0% vs. 5.6%, p < 0.001) and bronchopulmonary dysplasia (36.0% vs. 13.4%, p < 0.001). Infants receiving anti-Ureaplasma spp. treatment had a lower GA, lower BW, and more severe respiratory syndromes. However, the difference in respiratory manifestation became insignificant after adjusting for GA. Conclusions: GA and maternal vaginal Ureaplasma spp. colonization could be used to predict neonatal respiratory tract Ureaplasma spp. colonization. An elevated WBC count combined with normal CRP is a good marker of Ureaplasma spp. colonization/infection. It is conventional practice to start anti-Ureaplasma spp. treatment when infants present with a deteriorated respiratory condition. This practice warrants further investigation considering GA as a predominant intermediate variable. [ABSTRACT FROM AUTHOR]

Objectives: Network pharmacology is essential for understanding the multi-target and multi-pathway therapeutic mechanisms of traditional Chinese medicine. This study aims to evaluate the influence of database quality on target identification and to explore the therapeutic potential of rhynchophylline (Rhy) in treating overactive bladder (OAB). Methods: An OAB dataset was constructed through extensive literature screening. Using this dataset, we applied network pharmacology to predict potential targets for Rhy, which is known for its therapeutic effects but lacks a well-defined target profile. Predicted targets were validated through in vitro experiments, including DARTS and CETSA. Results: Our analysis identified Rhy as a potential modulator of the M3 receptor and TRPM8 channel in the treatment of OAB. Validation experiments confirmed the interaction between Rhy and these targets. Additionally, the GeneCards database predicted other targets that are not directly linked to OAB, corroborated by the literature. Conclusions: We established a more accurate and comprehensive dataset of OAB targets, enhancing the reliability of target identification for drug treatments. This study underscores the importance of database quality in network pharmacology and contributes to the potential therapeutic strategies for OAB. [ABSTRACT FROM AUTHOR]

PM2.5 pollution poses an important threat to the atmospheric environment and human health. To precisely forecast PM2.5 concentration, this study presents an innovative combined model: EMD-SE-GWO-VMD-ZCR-CNN-LSTM. First, empirical mode decomposition (EMD) is used to decompose PM2.5, and sample entropy (SE) is used to assess the subsequence complexity. Secondly, the hyperparameters of variational mode decomposition (VMD) are optimized by Gray Wolf Optimization (GWO) algorithm, and the complex subsequences are decomposed twice. Next, the sequences are divided into high-frequency and low-frequency parts by using the zero crossing rate (ZCR); the high-frequency sequences are predicted by a convolutional neural network (CNN), and the low-frequency sequences are predicted by a long short-term memory network (LSTM). Finally, the predicted values of the high-frequency and low-frequency sequences are reconstructed to obtain the final results. The experiment was conducted based on the data of 1009A, 1010A, and 1011A from three air quality monitoring stations in the Beijing area. The results indicate that the R2 value of the designed model increased by 2.63%, 0.59%, and 1.88% on average in the three air quality monitoring stations, respectively, compared with the other single model and the mixed model, which verified the significant advantages of the proposed model. [ABSTRACT FROM AUTHOR]

Rural tourism is a new driving force for achieving rural revitalization and development, promoting rural economic prosperity, and serving as a new industrial approach to achieving the transformation and upgrading of farmers' livelihoods. This paper focuses on Manas County as the research subject and employs farmer questionnaire interviews and participatory rural appraisal methods to categorize villages into four types: "scenic area-dependent" villages, "industry-dependent" villages, "folklore-dependent" villages, and "suburban-dependent" villages. Based on the sustainable livelihood analysis framework proposed by the Department for International Development, we developed a sustainable livelihood framework for farm households in Manas County and analyzed the effects of rural tourism on farm households' livelihood capital and livelihood strategies as the watershed of the rural tourism takes shape, taking livelihood capital and livelihood strategies as the entry point. The factors influencing the livelihood capital and livelihood options of farm households in the context of rural tourism are analyzed. The results show that: (1) The overall livelihood capital of farmers engaging in rural tourism is significantly greater than that of farmers not participating in rural tourism. Additionally, variations exist in the livelihood capital of different types of rural tourism within villages. (2) Participation in rural tourism significantly influences farmers' choices of livelihood strategies. Compared with individuals who do not engage in rural tourism, those involved in rural tourism are more likely to opt for self-management livelihood strategies. The proportion of self-managed farmers in villages based around scenic areas is the highest. (3) The effect of rural tourism on farm households in Manas County is generally positive, and various types of villages yield different impact effects. (4) Participation in rural tourism significantly increases the possibility of farmers choosing self-management livelihood strategies, while it reduces the possibility of farmers choosing agriculture-oriented livelihood strategies. In all villages, except those dependent on industry, the decision to engage in rural tourism significantly impacts farmers' selection of self-management livelihood strategies. [ABSTRACT FROM AUTHOR]

Prediction of fine particulate matter with particle size less than 2.5 µm (PM2.5) is an important component of atmospheric pollution warning and control management. In this study, we propose a deep learning model, namely, a spatiotemporal weighted neural network (STWNN), to address the challenge of poor long-term PM2.5 prediction in areas with sparse and uneven stations. The model, which is based on convolutional neural network–bidirectional long short-term memory (CNN–Bi-LSTM) and attention mechanisms and uses a geospatial data-driven approach, considers the spatiotemporal heterogeneity effec It is correct.ts of PM2.5. This approach effectively overcomes instability caused by sparse station data in forecasting daily average PM2.5 concentrations over the next week. The effectiveness of the STWNN model was evaluated using the Xinjiang Uygur Autonomous Region as the study area. Experimental results demonstrate that the STWNN exhibits higher performance (RMSE = 10.29, MAE = 6.4, R2 = 0.96, and IA = 0.81) than other models in overall prediction and seasonal clustering. Furthermore, the SHapley Additive exPlanations (SHAP) method was introduced to calculate the contribution and spatiotemporal variation of feature variables after the STWNN prediction model. The SHAP results indicate that the STWNN has significant potential in improving the performance of long-term PM2.5 prediction at the regional station level. Analyzing spatiotemporal differences in key feature variables that influence PM2.5 provides a scientific foundation for long-term pollution control and supports emergency response planning for heavy pollution events. [ABSTRACT FROM AUTHOR]

The fundamental component of the ecological compensation system, as well as the crucial basis for its efficient functioning, is calculating the ecological compensation amount and establishing the ecological compensation standard. This study integrates the ecological footprint with natural capital monetization and other methods by introducing a natural capital accounting system. From the standpoint of natural capital supply and demand, it also builds an accounting framework for ecological compensation standards that is standardized, dynamic, and regionally differentiated while taking local socioeconomic aspects into account. We determined the amount of ecological compensation by using Xinjiang as the research object and calculating and analyzing the features of regional and temporal changes in the monetary and physical quantities of natural capital in Xinjiang from 2010 to 2020. The findings show that from 2010 to 2020, Xinjiang's ecological footprint increased by 1.26 times in physical terms and 1.21 times in monetary terms and that its ecological carrying capacity increased by 4.13% in physical terms and 9.42% in monetary terms. The ecological deficit continues to grow in physical and monetary terms, with a per capita ecological deficit in 2020 of 19.92 s-nha/cap and 70,100 CNY/cap in physical and monetary terms, respectively. The amount of ecological compensation required to be paid in Xinjiang increased from CNY 5659 million to CNY 10,259 million, and the per capita ecological compensation payment standard increased from 259.42 CNY/cap/yr to 396.11 CNY/cap/yr. In summary, Xinjiang's natural capital supply is insufficient to meet the demand for consumption, and the ecological deficit is growing with time, necessitating the payment of ecological compensation. The study's results lay the foundation for formulating and implementing ecological compensation policies in Xinjiang and provide theoretical support for constructing ecological civilization in Xinjiang. In addition, the ecological compensation accounting framework constructed in this study organically integrates natural capital theory, ecosystem services, and socioeconomic influencing factors, which enriches the methodology of accounting for ecological compensation standards, and, at the same time, can be used as a paradigm of a dynamic and equitable ecological compensation accounting framework to further promote its use at different scales and regions. [ABSTRACT FROM AUTHOR]

Animal models of metabolic disorders are essential to studying pathogenic mechanisms and developing therapies for diabetes, but the induction protocols vary, and sexual dimorphism often exists. In a chronic diabetic model of diet-induced obesity (DIO) and low-dose streptozotocin (STZ)-induced hyperglycemia, blood glucose and lipid profiles were measured. The high-fat (HF) diet damaged insulin sensitivity and increased triglycerides, total cholesterol, LDL-cholesterol, HDL-cholesterol, and liver lipid deposition. STZ increased blood glucose and liver fibrosis with less effects on blood lipids or liver lipid deposition. The combination of DIO and STZ treatments led to significant liver lipid deposition and fibrosis. Female mice showed delayed body weight gain on HF diet and resisted STZ-induced hyperglycemia. However, once they developed DIO, which occurs around 26 weeks of HF diet, the female mice were prone to STZ-induced hyperglycemia. In hindlimb ischemia, male mice in the DIO-STZ group showed significantly worse neovascularization compared with DIO or STZ groups. The DIO-STZ females showed significantly worse recovery than the DIO-STZ males. Our observations suggest that DIO-STZ is a plausible model for studying metabolic and cardiovascular disorders in obesity and diabetes. Moreover, the findings in female animals stress the need to assess sexual dimorphism and investigate the underlying mechanisms that contribute to the worse vasculopathy manifestations in females in metabolic models. [ABSTRACT FROM AUTHOR]

In order to achieve minimum energy consumption in computerized numerical control (CNC) lathe processing under the premise of ensuring the imposed roughness of the machined surface, a black hole-continuous ant colony optimization algorithm (BH-ACOR) is proposed to optimize the turning parameters. Taking turning specific energy and surface roughness as the optimization objectives, a turning test was designed. Subsequently, a multi-objective mathematical model of the cutting stage was formulated through the application of the least-squares method to fit the test data. The black hole algorithm was introduced to mitigate the shortcomings of the continuous-domain ant colony algorithm, which easily falls into a local optimum, so as to put forward a kind of BH-ACOR that is applicable to multi-objective optimization. The algorithm was applied to the multi-objective mathematical model in the turning stage to determine the optimal cutting parameters. Through simulation and test verification, the validity and practicability of the proposed method are further proved. [ABSTRACT FROM AUTHOR]

Thick red clay in northern China contains rich information about the uplifting of the Qinghai–Tibet Plateau, the drying process of Asian inland, the East Asian monsoon changes, and global cooling since 22 Ma. In comparison, the red clay widely distributed in southern China is generally much younger (<1 million years), thus limiting the paleoclimate and paleoenvironment reconstruction over a longer geological time. We conducted a comprehensive magnetic investigation on the Pliocene red clay of the core LQ11, located in the Yangtze Delta, to reveal its paleoclimate and paleoenvironment implications for the eastern China coast. Our results revealed that the Pliocene red clay in the Yangtze Delta has higher S-ratio and lower HIRM (Hard isothermal remanent magnetizations) values than Quaternary vermiculate red clay of hot–humid climate origin in southern China. This indicates a weaker transformation from maghemite to hematite during the process of pedogenesis. The lack of net-like white veins in the Pliocene red clay also indicates a relatively low intensity of pedogenesis. We believe that the Pliocene red clay, which is presently 250 m below the mean sea level, was formed in high-altitude topography before the Quaternary period, where paedogenic intensity was remarkably low. This finding shows rapid tectonic subsidence occurring on the eastern China coast since the late Pliocene and enriches the theoretical research on paleoenvironment reconstruction based on red clay. [ABSTRACT FROM AUTHOR]

The urban agglomeration plays a significant role in enhancing integrated regional development. Nevertheless, the expansion of urban agglomerations has demonstrated a lackluster ability to attract cities. Presently, finding solutions to stabilize the existing urban strength and effectively extend attraction to neighboring cities has become a crucial matter. This study adopts the enhanced comprehensive attraction model, fracture point model, and radiation radius model to examine the level of city attraction, intensity of radiation, and range of radiation in the northern and southern Xinjiang city clusters between 2010 and 2020. Based on the analysis, the following conclusions are drawn: (1) the comprehensive strength and city attraction of cities in the northern Xinjiang region is higher than that of the southern Xinjiang region; (2) the intensity of spatial connection between cities in the northern and southern Xinjiang regions is gradually increasing, and the intensity of spatial connection of cities in the northern Xinjiang region is significantly greater than that in the southern Xinjiang region. The intensity of spatial connection between cities in the northern and southern Xinjiang regions is significantly greater than that in the southern Xinjiang region; (3) the central role of the central cities in the northern and southern Xinjiang regions is weakening, the development of cities in the region is gradually becoming unified and coordinated, and regional integration is gradually being strengthened. This study reveals the similarities and differences in urban development in the north and south of Xinjiang and provides important theoretical reference value for regional urban development. [ABSTRACT FROM AUTHOR]

In this study, the co-combustion of coal and biomass, and the tri-combustion of coal, biomass, and oil sludge in a 130 t h−1 circulating fluidized bed (CFB) boiler are investigated via the computational particle fluid dynamics (CPFD) approach. Furthermore, the effect of biomass feeding position is also comprehensively evaluated. The results show that for the co-combustion of coal and biomass, the O2 mole fraction at the furnace outlet rises from 0.0541 to 0.0640 as the biomass blending ratio enhances from 40% to 100%, while the CO2 mole fraction reduces from 0.1357 to 0.1267. The mole fraction of NOx and SO2 at the furnace outlet decreases from 4.5867 × 10−5 to 3.9096 × 10−5 and 2.8253 × 10−4 to 4.6635 × 10−5, respectively. For the tri-combustion of three fuels, the average NOx mole fraction initially grows quickly and then declines gradually, ranging from 4.1173 × 10−5 to 4.2556 × 10−5. The mole fraction of SO2 at the furnace outlet increases from 3.5176 × 10−4 to 4.7043 × 10−4 when the ratio of oil sludge rises from 10% to 20%. The uniformity of temperature and gas components distribution is "new inlet > secondary air inlet > feed inlet". As for the three inlet positions, the mole fractions of NOx at the furnace outlet are between 3.9096 × 10−5 and 5.1537 × 10−5, while those for SO2 are between 2.5978 × 10−4 and 2.5278 × 10−4. [ABSTRACT FROM AUTHOR]

Due to the large size of the bottom surface, the slipper pair of the large displacement piston pump (LDPP) will form a larger linear speed difference in the inner and outer positions of the slipper relative to the center of the swash plate during high-speed rotation. It is more likely to lead to the slipper overturning, which makes the slipper partially worn. To make improvements, the comprehensive performance of the slipper pair of the LDPP, the motion law of the slipper pair of the LDPP was explored. Firstly, a mathematical model of the oil film thickness of the slipper pair of the LDPP under the state of residual compression force is established, based on the consideration of the linear velocity difference formed by the high-speed rotation of the large bottom surface slipper and the theory of dynamics and thermodynamics. Secondly, the impact of rotational speed, piston chamber pressure and oil temperature on the oil film thickness of the slipper pair was simulated and analyzed. Finally, to measure the oil film thickness of the slipper pair, oil film thickness measuring equipment was created, and the accuracy of the mathematical model was verified. The study revealed the changing rules of the oil film thickness and tilt angle of the bottom surface of the slipper pair under various working conditions. The consistency of the simulation and test findings demonstrates that the mathematical model can accurately describe influencing elements and changing rules of the LDPP slipper pair's oil film lubrication characteristics. [ABSTRACT FROM AUTHOR]

Deep-sea submersibles carry limited energy sources, so a high efficiency of the equipment is required to improve endurance. In the deep-sea environment, the hydraulic power source is filled with oil, which causes structural deformation of the power source and changes in the physical properties of the medium, leading to unknown changes in the efficiency characteristics of the power source. In order to explore the efficiency characteristics of the deep-sea hydraulic power source composed of a gear pump and a DC (direct current) brushless motor in a variable sea depth environment, we undertook the following. First, considering the effects of seawater pressure and temperature on the physical properties of the medium and the radial clearance deformation of the gear pump, a mathematical model for the total efficiency of the hydraulic power source was established. The results indicate that the deformation of the pump body is mainly determined by the seawater pressure and working pressure. Subsequently, by analyzing the effects of the two factors on the efficiency of the power source, respectively, when the oil temperature range is large enough, the total efficiency of the power source will increase and then decrease under six sea depths; the total efficiency of the power source decreases with the increase in the rotational speed. However, in a land environment, the trend of the efficiency characteristics of the power source is opposite to that of the remaining six deep-sea environments, both in terms of oil temperature and rotational speed. Finally, the efficiency trend of the power source with changes in sea depth under rated conditions was obtained. Under different sea depth ranges, the optimal operating oil temperatures and suitable rotational speed ranges of the power source could be obtained. This paper could provide a certain theoretical basis for the research and development of deep-sea equipment. [ABSTRACT FROM AUTHOR]

The low-temperature environment of the Tibetan Plateau presents a technical challenge to composting. This study screened cold-adapted microbes with strong degradation ability and selected five strains to experimentally test and evaluate composting cattle manure in a natural environment in the plateau region. The results showed that both the control and the treatment groups had a slow temperature rise at the beginning of the composting. However, after the first turning, the temperature rise in the inoculated group accelerated. The inoculation of cold-adapted bacteria increased the OM loss in the compost by 8.6%, decreased the retention of nitrogen of the compost by 3%, and increased the seed germination index (GI) value from 44.4% to 73.9%. Microbial community structure analysis showed that the relative abundance of Psychrobacter was more than 50% at the beginning of the composting in the two experimental groups. The cold-adapted microbial inoculation increased the diversity of the microbial community, i.e., Truepera and Luteimona, and the abundance of specific microorganisms during the thermophilic and maturation stages. This study demonstrates that inoculation of cold-adapted bacteria improves the maturity and efficiency of cattle manure composting in a natural plateau environment. [ABSTRACT FROM AUTHOR]

Introduction: It is urgent to make a rapid screening of infants at the highest risk for bronchopulmonary dysplasia (BPD) via some succinct postnatal biomarkers, such as Ureaplasma Urealyticum (UU) infection and chest radiograph images. Methods: A retrospective study was performed. Moderate to severe BPD or death was set as the main outcome. The association between putative variables and the main outcome were assessed by bivariate analyses and logistic regression. Results: A total of 134 infants were enrolled. Bivariate analyses showed the gestational age, birth weight, appearances of diffuse opacities or grid shadows/interstitial opacities or mass opacities or cystic lucencies on chest radiographic images, a ductal diameter ≥1.5 mm and whether UU infection was associated with BPD. After adjustment by logistic regression, the risk of BPD with gestational age, sex and specific chest-radiographic manifestations remained significant. Conclusions: Chest radiograph images (appearance of diffuse opacities or grid shadows/interstitial opacities or mass opacities or cystic lucencies) could provide a quick prediction of developing BPD in clinical practice, in addition to gestational age and sex. UU infection was not an independent risk factor for BPD. [ABSTRACT FROM AUTHOR]

Green manufacturing has become a new production mode for the development and operation of modern and future manufacturing industries. The flexible job shop scheduling problem (FJSP), as one of the key core problems in the field of green manufacturing process planning, has become a hot topic and a difficult issue in manufacturing production research. In this paper, an improved multi-objective wolf pack algorithm (MOWPA) is proposed for solving a multi-objective flexible job shop scheduling problem with transportation constraints. Firstly, a multi-objective flexible job shop scheduling model with transportation constraints is established, which takes the maximum completion time and total energy consumption as the optimization objectives. Secondly, an improved wolf pack algorithm is proposed, which designs individual codes from two levels of process and machine. The precedence operation crossover (POX) operation is used to improve the intelligent behavior of wolves, and the optimal Pareto solution set is obtained by introducing non-dominated congestion ranking. Thirdly, the Pareto solution set is selected using the gray relational decision analysis method and analytic hierarchy process to obtain the optimal scheduling scheme. Finally, the proposed algorithm is compared with other algorithms through a variety of standard examples. The analysis results show that the improved multi-objective wolf pack algorithm is superior to other algorithms in terms of solving speed and convergence performance of the Pareto solution, which shows that the proposed algorithm has advantages when solving FJSPs. [ABSTRACT FROM AUTHOR]

Simple Summary: Previous studies have proved that brown planthopper (BPH), Nilaparvata lugens, a piercing–sucking herbivore, activates strong local defenses in rice. However, whether a BPH infestation elicits systemic responses in rice remains largely unknown. By detecting the change in transcript levels of 12 jasmonic acid (JA) and/or salicylic acid (SA) signaling-responsive marker genes in different tissues of rice plants upon a BPH attack, we demonstrate that an infestation of gravid BPH females systemically activates JA- and SA-dependent defenses in rice, which may in turn influence the composition and structure of the community in the rice ecosystem. It has been well documented that an infestation of the piercing–sucking herbivore, brown planthopper (BPH), Nilaparvata lugens, activates strong local defenses in rice. However, whether a BPH infestation elicits systemic responses in rice remains largely unknown. In this study, we investigated BPH-induced systemic defenses by detecting the change in expression levels of 12 JA- and/or SA-signaling-responsive marker genes in different rice tissues upon a BPH attack. We found that an infestation of gravid BPH females on rice leaf sheaths significantly increased the local transcript level of all 12 marker genes tested except OsVSP, whose expression was induced only weakly at a later stage of the BPH infestation. Moreover, an infestation of gravid BPH females also systemically up-regulated the transcription levels of three JA-signaling-responsive genes (OsJAZ8, OsJAMyb, and OsPR3), one SA-signaling-responsive gene (OsWRKY62), and two JA- and SA- signaling-responsive genes (OsPR1a and OsPR10a). Our results demonstrate that an infestation of gravid BPH females systemically activates JA- and SA-dependent defenses in rice, which may in turn influence the composition and structure of the community in the rice ecosystem. [ABSTRACT FROM AUTHOR]

Composting is an effective way to dispose of agricultural waste; however, its application is limited in the winter and in areas with low average annual temperatures. This study screened out a composite microbial agent (CMA) including Bacillus (B.) cereus QS7 and B. pumilus QM6 that could grow at 10–15 °C and investigated the effects of the CMA as an inoculant on the physicochemical parameters and microbial communities of compost made from mushroom residue mixed with sheep manure. The results showed that CMA inoculation prolonged the days of high temperature above 60 °C. When the ratio of mushroom residue to sheep manure was at 9:1, compost increased the accumulation of nitrogen, and reduced the electrical conductivity (EC). Under this ratio, the inoculation of CMA accelerated the degradation of organic matter (OM) (decreased by 12.22%) and total organic carbon (TOC) (decreased by 8.13%) and increased the germination rate and seed germination index (GI) to 100% and 106.86, respectively. Microbial community structure analysis showed that the relative abundance of Flavobacterium was 30.62% on day 15 and was higher after CMA inoculation in the high mushroom residue condition, which was higher than that of other groups, and the relative abundance of thermophilic fungi increased. This study demonstrated that CMA improves the quality and efficiency of mushroom residue and sheep manure composting, and it provides evidence to improve the efficiency of low-temperature composting. [ABSTRACT FROM AUTHOR]

The processes of sugarcane tillering and ratooning, which directly affect the yield of plant cane and ratoon, are of vital importance to the population establishment and the effective stalk number per unit area. In the present study, the phenotypic data of 285 F1 progenies from a cross of sugarcane varieties YT93-159 × ROC22 were collected in eight environments, which consisted of plant cane and ratoon cultivated in three different ecological sites. The broad sense heritability (H2) of the tillering and the ratoon sprouting was 0.64 and 0.63, respectively, indicating that they were middle to middle-high heritable traits, and there is a significantly positive correlation between the two traits. Furthermore, a total of 26 quantitative trait loci (QTLs) related to the tillering ability and 11 QTLs associated with the ratooning ability were mapped on two high-quality genetic maps derived from a 100K SNP chip, and their phenotypic variance explained (PVE) ranged from 4.27–25.70% and 6.20–13.54%, respectively. Among them, four consistent QTLs of qPCTR-R9, qPCTR-Y28, qPCTR-Y60/qRSR-Y60 and PCTR-Y8-1/qRSR-Y8 were mapped in two environments, of which, qPCTR-Y8-1/qRSR-Y8 had the PVEs of 11.90% in the plant cane and 7.88% in the ratoon. Furthermore, a total of 25 candidate genes were identified in the interval of the above four consistent QTLs and four major QTLs of qPCTR-Y8-1, qPCTR-Y8-2, qRSR-R51 and qRSR-Y43-2, with the PVEs from 11.73–25.70%. All these genes were associated with tillering, including eight transcription factors (TFs), while 15 of them were associated with ratooning, of which there were five TFs. These QTLs and genes can provide a scientific reference for genetic improvement of tillering and ratooning traits in sugarcane. [ABSTRACT FROM AUTHOR]

In China, nitrogen (N) fertilizer is excessively used in sugarcane planting areas, while the nitrogen use efficiency (NUE) of sugarcane is relatively low. Mining and identifying the key genes in response to low N stress in sugarcane can provide useful gene elements and a theoretical basis for developing sugarcane varieties with high NUE. In our study, RNA-Seq combined with qRT-PCR analysis revealed that the ScAMT1.1 gene responded positively to low N stress, resulting in the stronger low N tolerance and high NUE ability of sugarcane cultivar ROC22. Then, ScAMT1.1 was cloned from sugarcane. The full-length cDNA of the ScAMT1.1 gene is 1868 bp, containing a 1491 bp open reading frame (ORF), and encoding 496 amino acids. ScAMT1.1 belongs to the AMT superfamily and shares 91.57% homologies with AMT1.1 from Oryza sativa. Furthermore, it was stably overexpressed in rice (O. sativa). Under low N treatment, the plant height and the fresh weight of the ScAMT1.1-overexpressed transgenic rice were 36.48% and 51.55% higher than that of the wild-type, respectively. Both the activity of ammonium assimilation key enzymes GS and GDH, and the expression level of ammonium assimilation key genes, including GS1.1, GS1.2, GDH, Fd-GOGAT, and NADH-GOGAT2 in the transgenic plants, were significantly higher than that of the wild-type. The grain number and grain yield per plant in the transgenic rice were 6.44% and 9.52% higher than that of the wild-type in the pot experiments, respectively. Taken together, the sugarcane ScAMT1.1 gene has the potential to improve ammonium assimilation ability and the yield of transgenic rice under low N fertilizer conditions. This study provided an important functional gene for improving sugarcane varieties with high NUE. [ABSTRACT FROM AUTHOR]

Rapid social development has led to serious air pollution problems in cities, and air pollutants, including gaseous pollutants and particulate matter, have an important impact on climate, the environment, and human health. This study analyzed the characteristics, potential sources, and causes of air pollution in the Wu-Chang-Shi urban cluster. The results showed that NO2, CO, SO2, PM10, and PM2.5 had a tendency to decrease, while O3 showed an increasing trend. The concentrations of SO2, NO2, CO, PM2.5, and PM10 showed the highest values in winter and the lowest values in summer, with similar seasonal variations. However, the concentration of O3 was highest in the summer and lowest in the winter. Compared with the pollutant concentrations in other Chinese cities, PM2.5, PM10, and NO2 are more polluted in the Wu-Chang-Shi urban. Meteorological factors have a greater impact on pollutant concentrations, with higher concentrations of major pollutants observed when wind speeds are low and specific wind directions are observed, and higher secondary pollutant O3 concentrations observed when wind speeds are low and specific wind directions are observed. The backward trajectory and concentration weighting analysis show that the particulate pollutants in the Wu-Chang-Shi urban in winter mainly come from Central Asia and surrounding cities. O3 showed an increasing trend before and after the novel coronavirus outbreak, which may be related to changes in NOX, volatile organic compounds, and solar radiation intensity, and the concentrations of SO2, NO2, CO, PM10, and PM2.5 showed an overall decreasing trend after the outbreak and was smaller than before the outbreak, which is related to the reduction of industrial and anthropogenic source emissions during the outbreak. [ABSTRACT FROM AUTHOR]

Sugarcane brown stripe (SBS), caused by the fungal pathogen Helminthosporium stenospilum, is one of the most serious threats to sugarcane production. However, its outbreaks and epidemics require suitable climatic conditions, resulting in the inefficient improvement of the SBS resistance by phenotype selection. The sugarcane F1 population of SBS-resistant YT93-159 × SBS-susceptible ROC22 was used for constructing the bulks. Bulked segregant RNA-seq (BSR-seq) was then performed on the parents YT93-159 (T01) and ROC22 (T02), and the opposite bulks of 30 SBS-susceptible individuals mixed bulk (T03) and 30 SBS-resistant individuals mixed bulk (T04) collected from 287 F1 individuals. A total of 170.00 Gb of clean data containing 297,921 SNPs and 70,426 genes were obtained. Differentially expressed genes (DEGs) analysis suggested that 7787 and 5911 DEGs were identified in the parents (T01 vs. T02) and two mixed bulks (T03 vs. T04), respectively. In addition, 25,363 high-quality and credible SNPs were obtained using the genome analysis toolkit GATK for SNP calling. Subsequently, six candidate regions with a total length of 8.72 Mb, which were located in the chromosomes 4B and 7C of sugarcane wild species Saccharum spontaneum, were identified, and 279 genes associated with SBS-resistance were annotated by ED algorithm and ΔSNP-index. Furthermore, the expression profiles of candidate genes were verified by quantitative real-time PCR (qRT-PCR) analysis, and the results showed that eight genes (LRR-RLK, DHAR1, WRKY7, RLK1, BLH4, AK3, CRK34, and NDA2) and seven genes (WRKY31, CIPK2, CKA1, CDPK6, PFK4, CBL2, and PR2) of the 20 tested genes were significantly up-regulated in YT93-159 and ROC22, respectively. Finally, a potential molecular mechanism of sugarcane response to H. stenospilum infection is illustrate that the activations of ROS signaling, MAPK cascade signaling, Ca2+ signaling, ABA signaling, and the ASA-GSH cycle jointly promote the SBS resistance in sugarcane. This study provides abundant gene resources for the SBS resistance breeding in sugarcane. [ABSTRACT FROM AUTHOR]

The reference value of the oil film thickness and friction coefficient of the slipper pair is critical to the development of the piston pump, especially for 750 mL/r displacement piston pumps. To explore the computing method and range of the reference value mentioned applicable to 750 mL/r displacement piston pumps, this study aims to propose the modified calculation model of the oil film thickness based on the real clearance flowrate and obtain the value range of the friction coefficient of the slipper pair. Through the friction test of the slipper pair, the mean deviation ratio of the oil film thickness between the modified value, theoretical value, and the measured value was calculated and compared, respectively. The variation law of the friction under the influence of different speeds and working pressures was analyzed. Finally, the range of the equivalent friction coefficient with the upper and lower limit surfaces was obtained. The results show that the mean deviation ratio between the modified oil film thickness value and the measured value is mainly within 6%, while that of the theoretical method is mainly from 6% to 8%, and the mean of the difference between the two deviation ratios is about 3%, verifying the feasibility of the modified model used for the calculation of the reference value. Meanwhile, the value of the equivalent friction coefficient fluctuates in the range of 0.006–0.018, which is affected more significantly by the working pressure than the speed, suggesting that the working pressure can be given priority as the design basis of the friction coefficient for 750 mL/r displacement piston pumps. [ABSTRACT FROM AUTHOR]

Chemical nitrogen (N) fertilizer is widely used in sugarcane production, especially in China and India. Understanding the molecular mechanisms and mining miRNAs and their target genes associated with nitrogen use efficiency (NUE) in sugarcane can aid in developing the N-efficient varieties, and thus is beneficial to reduce N fertilizer application. In this study, the root miRNA database of N-efficient sugarcane variety ROC22 under low N stress (0.3 mM NH4NO3) for 3 h was constructed, along with their transcriptome-rearranged data. KEGG analysis indicated that those candidate target genes, corresponding to differentially expressed miRNAs, were mainly enriched in N metabolism, amino acid metabolism, carbohydrate metabolism, photosynthesis, and hormone signal transduction pathways. It was found that under low N stress for 0–24 h, there was a negative correlation between miR168 and SPX, along with miR396 and acnA. Furthermore, the expression of miR156 in the roots of ROC22 was significantly up-regulated under low N treatment. Compared with the wild-type, the Arabidopsis plants overexpressing sugarcane miR156 exhibited significantly improved length and surface area of roots, while the expression of one NO3− transporter gene NRT1.1, three N assimilation key genes (NR1, NIR1, and GS), and the activity of two N assimilation key enzymes (NR and GS) were up-regulated under low N treatment. It can be reasonably deduced that sugarcane miR156 can enhance the nitrogen assimilation ability of the overexpressed Arabidopsis plants under low N application, and thus has a potential ability for improving sugarcane NUE. The present study should be helpful for understanding the molecular regulatory network in the N-efficient sugarcane genotype responding to low N stress and could provide the candidate miRNAs with a potential function in improving sugarcane NUE. [ABSTRACT FROM AUTHOR]

Glaciers are susceptible indicators of climate change and crucial parts of the world's water cycle. In the context of global warming, we took the Urumqi Glacier No.1 (UG1) as an example, which is situated at the source of the Urumqi River on the northern slope of the Tianshan Mountains, Xinjiang, combined with the climate data of Daxigou Meteorological Station from 1980 to 2020, and the change of glacier mass balance and its response to extreme climate are discussed. The results suggest that the glacier mass balance of UG1 showed a downward trend over the studied 41-year period, and the mass loss increased. The cumulative glacier mass balance value was −19,776 mm w.e., and the average annual value was −482 mm w.e.a−1. The Mann-Kendall trend test showed that the change point occurred around 1994, and the mass balance of UG1 became more negative after 1994. In the same period, the changing mass balance trend of UG1 was not the same in different seasons. The inter-annual variation of summer mass balance was drastic, showing a marked downward trend; the inter-annual change of winter mass balance was small, showing a slight uptrend. The changing of extreme climate indices where UG1 is located showed that only TX90p and TX10p changed observably from 1980 to 2020, and the extreme precipitation indices changed evidently and had been on the rise. The changing trend of extreme climate indices indicated that the temperature was rising, the warming was significant, and the precipitation was increasing. During 1980–2020, the glacier mass balance was substantially correlated with the extreme temperature indices (TX90p, TXx) but not with the extreme precipitation indices. Analyzing on a seasonal scale, the summer mass balance was memorably correlated with the extreme temperature indices (TX90p, TX10p, TXx), and the correlation coefficient between winter mass balance and the extreme precipitation index R95p and winter precipitation was in the range 0.36~0.40 (p < 0.05). According to the correlation between glacier mass balance and extreme climate indices, the summer mass balance was mainly affected by temperature, and the winter mass balance was affected primarily by precipitation. [ABSTRACT FROM AUTHOR]

Sugarcane smut is a major fungal disease caused by Sporisorium scitamineum, which seriously reduces the yield and quality of sugarcane. In this study, 36 transcriptome data were collected from two sugarcane genotypes, YT93-159 (resistant) and ROC22 (susceptible) upon S. scitamineum infection. Data analysis revealed 20,273 (12,659 up-regulated and 7614 down-regulated) and 11,897 (7806 up-regulated and 4091 down-regulated) differentially expressed genes (DEGs) in YT93-159 and ROC22, respectively. A co-expression network was then constructed by weighted gene co-expression network analysis (WGCNA), which identified 5010 DEGs in 15 co-expressed gene modules. Four of the 15 modules, namely, Skyblue, Salmon, Darkorange, and Grey60, were significantly associated with smut resistance. The GO and KEGG enrichment analyses indicated that the DEGs involving in these four modules could be enriched in stress-related metabolic pathways, such as MAPK and hormone signal transduction, plant-pathogen interaction, amino acid metabolism, glutathione metabolism, and flavonoid, and phenylpropanoid biosynthesis. In total, 38 hub genes, including six from the Skyblue module, four from the Salmon module, 12 from the Darkorange module, and 16 from the Grey60 module, were screened as candidate hub genes by calculating gene connectivity in the corresponding network. Only 30 hub genes were amplifiable with RT-qPCR, of which 27 were up-regulated upon S. scitamineum infection. The results were consistent with the trend of gene expression in RNA-Seq, suggesting their positive roles in smut resistance. Interestingly, the expression levels of AOX, Cyb5, and LAC were higher in ROC22 than in YT93-159, indicating these three genes may act as negative regulators in response to S. scitamineum infection. This study revealed the transcriptome dynamics in sugarcane challenged by S. scitamineum infection and provided gene targets for smut resistance breeding in sugarcane. [ABSTRACT FROM AUTHOR]

The dehydration-responsive element-binding protein (DREB) is a subgroup member of the AP2/ERF family and actively participates in the response of plants to abiotic stress. Although DREB genes have been studied in a variety of plant species, there are few reports of DREB genes in sugarcane (Saccharum spp.). In this study, a novel full-length cDNA sequence of the ScDREB2B-1 gene was cloned from the Saccharum hybrid ROC22, whose encoding protein contained only one AP2-conserved domain and was clustered into the DREB (A-2) subgroup. The diverse promoter elements in the ScDREB2B-1 gene and the accumulated transcripts of its homologous gene (SsAP2/ERF-107) in S. spontaneum under drought stress suggest that the ScDREB2B-1 gene may play a role in drought response. In addition, reverse transcription quantitative PCR analysis showed that the expression level of the ScDREB2B-1 gene was upregulated in the root and leaf of ROC22 under polyethylene glycol, sodium chloride and abscisic acid (ABA) treatments. The yeast two-hybrid experiment demonstrated that ScDREB2B-1 had transcriptional self-activation activity. Compared with wild-type plants, the overexpression of the ScDREB2B-1 gene improved the drought tolerance of the transgenic Nicotiana benthamiana by activating the ABA pathway to enhance the expression of the ABA-responsive gene (NbNCED) and ABA content, regulate the intracellular reactive oxygen species (ROS) level (enhance the transcripts of ROS synthase-related gene NbRbohB and the activities of catalase, peroxidase and superoxide dismutase) and increase the relative water content, proline content and expression level of osmotic stress-related genes (NbERD and NbLEA). Collectively, our data indicate that ScDREB2B-1 is a stress-inducible and ABA-responsive transcription factor gene that responds to drought stress by regulating ABA signaling, ROS levels and stress-related gene expression. This study contributes to a better understanding of the biological function of ScDREB2B-1, which could serve as a foundation for future resistance breeding in sugarcane. [ABSTRACT FROM AUTHOR]

Many nonfinancial firms in China invest increasingly in financial assets. To understand the driving factors behind this phenomenon, this paper examines the effect of environmental, social, and governance (ESG) performance on corporate financialization. The empirical results show that ESG performance has a positive effect on corporate financialization, suggesting that ESG activities are a tool for firms to seek financial arbitrage. Further examination confirms that corporate financialization of Chinese nonfinancial listed firms is motivated mainly by maximizing short-term financial returns, rather than reserving funds for long-term development. Heterogeneity analysis shows that the positive effect is more significant in non-state-owned firms and in firms located in regions with a low degree of marketization. This study enriches the existing literature on the economic consequences of ESG performance and the influential factors of corporate financialization and provides practical guidance for government regulators to strengthen stricter regulation on ESG activities and financial asset investment to ensure sustainable and healthy economic development. [ABSTRACT FROM AUTHOR]

Triple-negative breast cancers are highly aggressive with an overall poor prognosis and limited therapeutic options. We had previously investigated the role of mdig, an oncogenic gene induced by some environmental risk factors, on the pathogenesis of breast cancer. However, a comprehensive analysis of the proteomic profile affected by mdig in triple-negative breast cancer has not been determined yet. Using label-free bottom-up quantitative proteomics, we compared wildtype control and mdig knockout MDA-MB-231 cells and identified the proteins and pathways that are significantly altered with mdig deletion. A total of 904 differentially expressed (p < 0.005) proteins were identified in the KO cells. Approximately 30 pathways and networks linked to the pathogenicity of breast cancer were either up- or downregulated, such as EIF2 signaling, the unfolded protein response, and isoleucine degradation I. Ingenuity Pathway Analysis established that the differentially expressed proteins have relevant biological actions in cell growth, motility, and malignancy. These data provide the first insight into protein expression patterns in breast cancer associated with a complete disruption of the mdig gene and yielded substantial information on the key proteins, biological processes, and pathways modulated by mdig that contribute to breast cancer tumorigenicity and invasiveness. [ABSTRACT FROM AUTHOR]

The VQ motif-containing proteins play a vital role in various processes such as growth, resistance to biotic and abiotic stresses and development. However, there is currently no report on the VQ genes in sugarcane (Saccharum spp.). Herein, 78 VQ genes in Saccharum spontaneum were identified and classified into nine subgroups (I-IX) by comparative genomic analyses. Each subgroup had a similar structural and conservative motif. These VQ genes expanded mainly through whole-genome segmental duplication. The cis-regulatory elements (CREs) of the VQ genes were widely involved in stress responses, phytohormone responses and physiological regulation. The RNA-seq data showed that SsVQ gene expression patterns in 10 different samples, including different developmental stages, revealed distinct temporal and spatial patterns. A total of 23 SsVQ genes were expressed in all tissues, whereas 13 SsVQ genes were not expressed in any tissues. Sequence Read Archive (SRA) data showed that the majority of SsVQs responded to cold and drought stress. In addition, quantitative real-time PCR analysis showed that the SsVQs were variously expressed under salicylic acid (SA), jasmonic acid (JA), abscisic acid (ABA) and cold treatment. This study conducted a full-scale analysis of the VQ gene family in sugarcane, which could be beneficial for the functional characterization of sugarcane VQ genes and provide candidate genes for molecular resistance breeding in cultivated sugarcane in the future. [ABSTRACT FROM AUTHOR]

Modern sugarcane cultivars are highly polyploid with complex nuclear genomic genetic background, while their mitochondrion (mt) genomes are much simpler, smaller and more manageable and could provide useful phylogenetic information. In this study, the mt genome of a modern commercial cultivar A1 was sequenced via Illumina Hiseq XTen and PacBio Sequel platform. The assembled and annotated mitochondrial genomes of A1 were composed of two circular DNA molecules, one large and one small, which were named Chromosome 1 and Chromosome 2. The two distinct circular chromosomes of mitogenome construct is consisted with other sugarcane cultivars i.e., Saccharum officinarum Khon Kaen 3 and Saccharum spp. hybrids ROC22 and FN15. The Chromosome 1 of A1 mitogenome is 300,822 bp in length with the GC content of 43.94%, and 7.14% of Chromosome 1 sequences (21,468 nucleotides) are protein coding genes (PCGs) while 92.86% (279,354 nucleotides) are intergenic region. The length of Chromosome 2 is 144,744 bp with the GC content of 43.57%, and 8.20% of Chromosome 2 sequences (11,865 nucleotides) are PCGs while 91.80% (132,879 nucleotides) are intergenic region. A total of 43 genes are located on Chromosome 1, which contains 22 PCGs (six nad genes, four rps genes, four atp genes, three ccm genes, three cox genes, one mat gene and one mtt gene) and 21 non-coding genes including 15 tRNAs and 6 rRNAs. Chromosome 2 includes 18 genes in total, which contains 13 PCGs (four nad genes, three rps genes, two atp genes, one ccm gene, one cob gene, one cox gene and one rpl gene) and five non-coding genes (tRNA genes). Analysis of codon usage of 35 PCGs showed that codon ending in A/U was preferred. Investigation of gene composition indicated that the types and copy numbers of CDS genes, tRNAs and rRNAs of A1 and FN15 were identical. The cox1 gene has two copies and the trnP gene has one copy in A1, FN15 and ROC22 three lines, while there is only one copy of cox1 and two copies of trnP in S. officinarum Khon Kaen 3. In addition, S. officinarum Khon Kaen 3 have no nad1 gene and rps7 gene. 100 sequence repeats, 38 SSRs and 444 RNA editing sites in A1 mt genome were detected. Moreover, the maximum likelihood phylogenetic analysis found that A1 were more closely related to S. spp. hybrid (ROC22 and FN15) and S. officinarum (Khon Kaen 3). Herein, the complete mt genome of A1 will provide essential DNA molecular information for further phylogenetic and evolutionary analysis for Saccharum and Poaceae. [ABSTRACT FROM AUTHOR]

Drought stress can dramatically impair woody plant growth and restrict the geographical distribution of many tree species. To better understand the dynamics between the response and mechanism of Cupressus gigantea to drought and post-drought recovery, a comparative analysis was performed, relying on physiological measurements, RNA sequencing (RNA-Seq) and two-dimensional gel electrophoresis (2-DE) proteins. In this study, the analyses revealed that photosynthesis was seriously inhibited, while osmolyte contents, antioxidant enzyme activity and non-enzymatic antioxidant contents were all increased under drought stress in seedlings. Re-watering led to a recovery in most of the parameters analyzed, mainly the photosynthetic parameters and osmolyte contents. Transcriptomic and proteomic profiling suggested that most of the differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) were specifically altered, and a few were consistently altered. Drought induced a common reduction in the level of DEGs and DEPs associated with photosynthesis. Notably, DEGs and DEPs involved in reactive oxygen species (ROS) scavenging, such as ascorbate oxidase and superoxide dismutase (SOD), showed an inverse pattern under desiccation. This study may improve our understanding of the underlying molecular mechanisms of drought resistance in C. gigantea and paves the way for more detailed molecular analysis of the candidate genes. [ABSTRACT FROM AUTHOR]

Mosaic is one of the most important sugarcane diseases, caused by single or compound infection of Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), and/or Sugarcane streak mosaic virus (SCSMV). The compound infection of mosaic has become increasingly serious in the last few years. The disease directly affects the photosynthesis and growth of sugarcane, leading to a significant decrease in cane yield and sucrose content, and thus serious economic losses. This review covers four aspects of sugarcane mosaic disease management: first, the current situation of sugarcane mosaic disease and its epidemic characteristics; second, the pathogenicity and genetic diversity of the three viruses; third, the identification methods of mosaic and its pathogen species; and fourth, the prevention and control measures for sugarcane mosaic disease and potential future research focus. The review is expected to provide scientific literature and guidance for the effective prevention and control of mosaic through resistance breeding in sugarcane. [ABSTRACT FROM AUTHOR]

Barite is an abundant sulfate mineral in nature. Especially, the variety of morphologies of barite is often driven by the mixing of Ba-bearing hydrothermal fluid and sulfate-bearing seawater around hydrothermal chimneys. In order to better understand the factors affecting the morphology and precipitation mechanism(s) of barite in seafloor hydrothermal systems, we synthesized barite by a new method of in-situ mixing of BaCl2 and Na2SO4 solutions at 200 °C while varying Ba concentrations and ratios of Ba2+/SO42−, and at room temperature for comparison. The results show that barite synthesized by in-situ mixing of BaCl2 and Na2SO4 solutions at 200 °C forms a variety of morphologies, including rod-shaped, granular, plate-shaped, dendritic, X-shaped, and T-shaped morphologies, while room temperature barites display relatively simple, granular, or leaf-like morphologies. Thus, temperature affects barite morphology. Moreover, dendritic barite crystals only occurred at conditions where Ba2+ is in excess of SO42− at the experimental concentrations. The dendritic morphology of barite may be an important typomorphic feature of barite formed in high-temperature fluids directly mixing with excess Ba2+ relative to SO42−. [ABSTRACT FROM AUTHOR]

Under the background of global climate change, the variation in the spatial distribution and ice volume of mountain glaciers have a profound influence on regional economic development and ecological security. The development of glaciers is like biological succession; when climate change approaches or exceeds the threshold of suitable conditions for glacier development, it will lead to changes in potential distribution pattern. Therefore, from the perspective of the "biological" characteristics of glaciers, it is a beneficial exploration and attempt in the field of glaciology to explore its potential distribution law with the help of the niche model. The maximum entropy model (MaxEnt) can explain the environmental conditions suitable for the survival of things by analyzing the mathematical characteristics and distribution laws of samples in space. According to glacier samples and the geographical environment data screened by correlation analysis and iterative calculation, the potential distribution pattern of Tianshan glaciers in China in reference years (1970–2000) was simulated by MaxEnt. This paper describes the contribution of geographical environmental factors to distribution of glaciers in Tianshan Mountains, quantifies the threshold range of factors affecting the suitable habitat of glaciers, and predicts the area variation and distribution pattern of glaciers under different climate scenarios (SSP1-2.6, SSP5-8.5) in the future (2040–2060, 2080–2100). The results show that the MaxEnt model has good adaptability to simulate the distribution of glaciers. The spatial heterogeneity of potential distribution of glaciers is caused by the spatio-temporal differences of hydrothermal combination and topographic conditions. Among the environmental variables, precipitation during the wettest month, altitude, annual mean temperature, and temperature seasonality have more significant effects on the potential distribution of glaciers. There is significant spatial heterogeneity in the potential distribution of glaciers in different watersheds, altitudes, and aspects. From the forecast results of glacier in various climatic scenarios in the future, about 18.16–27.62% of the total reference year glacier area are in an alternating change of melting and accumulation, among which few glaciers are increasing, but this has not changed the overall retreat trend of glaciers in the study area. Under the low emission scenario, the glacier area of the Tianshan Mountains in China decreased by 18.18% and 23.73% respectively in the middle and end of the 21st century compared with the reference years and decreased by 20.04% and 27.63%, respectively, under the high emission scenario, which showed that the extent of glacier retreat is more intense under the high emission scenario. Our study offers momentous theoretical value and practical significance for enriching and expanding the theories and analytical methods of the glacier change. [ABSTRACT FROM AUTHOR]

Sugarcane is an important sugar and bioenergy ethanol crop, and the hyperploidy has led to stagnant progress in sugarcane genome decipherment, which also hindered the genome-wide analyses of versatile lectin receptor kinases (LecRKs). The published genome of Saccharum spontaneum, one of the two sugarcane ancestor species, enables us to study the characterization of LecRKs and their responses to sugarcane leaf blight (SLB) triggered by Stagonospora tainanensis. A total of 429 allelic and non-allelic LecRKs, which were classified into evolved independently three types according to signal domains and phylogeny, were identified based on the genome. Regarding those closely related LecRKs in the phylogenetic tree, their motifs and exon architectures of representative L- and G-types were similar or identical. LecRKs showed an unequal distribution on chromosomes and more G-type tandem repeats may come from the gene expansion. Comparing the differentially expressed LecRKs (DELs) in response to SLB in sugarcane hybrid and ancestor species S. spontaneum, we found that the DEL number in the shared gene sets was highly variable among each sugarcane accession, which indicated that the expression dynamics of LecRKs in response to SLB were quite different between hybrids and particularly between sugarcane hybrid and S. spontaneum. In addition, C-type LecRKs may participate in metabolic processes of plant–pathogen interaction, mainly including pathogenicity and plant resistance, indicating their putative roles in sugarcane responses to SLB infection. The present study provides a basic reference and global insight into the further study and utilization of LecRKs in plants. [ABSTRACT FROM AUTHOR]

Sugarcane is an important sugar and potential energy crop, and the complexity of its genome has led to stagnant progress in genome decipherment and hindered the genome-wide analysis of the nucleotide binding site leucine-rich repeat (NLR) receptor until the genome of Saccharum spontaneum was published. From the genome of S. spontaneum, 724 allelic and non-allelic NLRs were identified and classified into five types (N, NL, CN, CNL, and P) according to domain architectures and integrity and at least 35 genes encoded non-canonical domains. The phylogenetic analysis indicated NLRs containing the coiled-coil (CC) domain separated from those without CC in six Poaceae species, including S. spontaneum. The motif analysis determined the characteristics and potential functions of the 137 representative non-allelic NLRs, especially the core motifs contained in the NBS and LRR domains, which indicated that motifs were regularly distributed among clades. Through transcription factor binding site (TFBS) profiles, we predicted that the most important transcription regulator of NLRs in sugarcane were ERF, MIKC_MADS, and C2H2. In addition, based on three sets of transcriptome data from two sugarcane hybrids and one S. spontaneum clone infected by the necrotrophic fungal pathogen Stagonospora tainanensis causing sugarcane leaf blight (SLB), the expression dynamics of NLRs responding to the infection in three sugarcane clones were compared. The different genetic background led to the significant difference of NLRs response to SLB in different sugarcane clones, and we got an inference of the potential mechanism of SLB resistance. These results provided a basic reference and new insights to further study and utilize the NLRs. [ABSTRACT FROM AUTHOR]

Understanding the oxidative mechanisms of chalcopyrite in various hydrothermal fluids is of great significance to improve copper extraction and to model the geochemical cycling of copper, iron, and sulfur. This paper investigated the effect of NaCl on the electrochemical oxidation of chalcopyrite at 340 °C and 21 MPa using polarization curves, electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and Raman spectroscopy. The experimental results showed that NaCl can promote chalcopyrite leaching. As NaCl concentration increases, the protective property of the oxidation layer degraded. In the absence of NaCl, the oxidation layer that consisted of CuSn, (n ≥ 1), probably with some Fe2O3 and Fe(OH)3 and also in the presence of NaCl, Fe2O3, is the principal oxidation product. More rapid ionic diffusion and further chemical reaction contributed to the improvement of chalcopyrite dissolution with increasing NaCl concentration. A dissolution mechanism is proposed in this paper to explain the chalcopyrite leaching processes which is dependent on NaCl concentration. [ABSTRACT FROM AUTHOR]

The Xinjiang Uyghur Autonomous Region of China is the largest arid region in Central Asia, and is heavily dependent on glacier melt in high mountains for water supplies. In this paper, glacier and climate changes in Xinjiang during the past decades were comprehensively discussed based on glacier inventory data, individual monitored glacier observations, recent publications, as well as meteorological records. The results show that glaciers have been in continuous mass loss and dimensional shrinkage since the 1960s, although there are spatial differences between mountains and sub-regions, and the significant temperature increase is the dominant controlling factor of glacier change. The mass loss of monitored glaciers in the Tien Shan has accelerated since the late 1990s, but has a slight slowing after 2010. Remote sensing results also show a more negative mass balance in the 2000s and mass loss slowing in the latest decade (2010s) in most regions. This needs further investigation on whether the slowing is general and continuing. In addition, glacier surging occurs more frequently in the Karakoram and Kunlun Mountains. [ABSTRACT FROM AUTHOR]

P25 loaded few layered molybdenum disulfide (MoS2) nanosheets (P25@MoS2) are successfully synthesized through a facile one-step hydrothermal process. The bi-catalytic activities, i.e., photocatalytic and electrocatalytic activities, of the as-prepared nanomaterials have been investigated. For the as-prepared products, the photocatalytic performances were investigated by degrading simulated pollutant under sunlight irradiation, and the hydrogen evolution reaction evaluated the electrocatalytic performances. The results indicate that P25@MoS2 possesses excellent activities in both photocatalysis and electrocatalysis. The presence of MoS2 broadens the light absorption range of P25 and improves the separation and transformation efficiency of photogenerated carriers, thus improving its photocatalytic performance. The existence of P25 inhibits the aggregation of MoS2 to form more dispersed MoS2 nanosheets with only few layers increasing its active sites. Thereby, the electrocatalytic performance is heightened. The excellent multifunction makes the as-prepared P25@MoS2 a promising material in the fields of environment and energy. [ABSTRACT FROM AUTHOR]

Polyploid Saccharum with complex genomes hindered the progress of sugarcane improvement, while their chloroplast genomes are much smaller and simpler. Chloroplast (cp), the vital organelle, is the site of plant photosynthesis, which also evolves other functions, such as tolerance to environmental stresses. In this study, the cp genome of two sugarcane ancestors Saccharum officinarum and S. spontaneum were sequenced, and genome comparative analysis between these two species was carried out, together with the photosynthetic ability. The length is 141,187 bp for S. officinarum and that is 7 bp longer than S. spontaneum, with the same GC content (38.44%) and annotated gene number (134), 13 with introns among them. There is a typical tetrad structure, including LSC, SSC, IRb and IRa. Of them, LSC and IRa/IRb are 18 bp longer and 6 bp shorter than those in S. spontaneum (83,047 bp and 22,795 bp), respectively, while the size of SSC is same (12,544 bp). Five genes exhibit contraction and expansion at the IR junctions, but only one gene ndhF with 29 bp expansion at the border of IRb/SSC. Nucleotide diversity (Pi) based on sliding window analysis showed that the single copy and noncoding regions were more divergent than IR- and coding regions, and the variant hotspots trnG-trnM, psbM-petN, trnR-rps14, ndhC-trnV and petA-psbJ in the LSC and trnL-ccsA in the SSC regions were detected, and petA-psbJ with the highest divergent value of 0.01500. Genetic distances of 65 protein genes vary from 0.00000 to 0.00288 between two species, and the selective pressure on them indicated that only petB was subjected to positive selection, while more genes including rpoC2, rps3, ccsA, ndhA, ndhA, psbI, atpH and psaC were subjected to purifying or very strong purifying selection. There are larger number of codons in S. spontaneum than that in S. officinarum, while both species have obvious codon preference and the codons with highest-(AUG) and lowest frequency (AUA) are same. Whilst, the most abundant amino acid is leucine in both S. officinarum and S. spontaneum, with number of 2175 (10.88% of total) and 2228 (10.90% of total) codons, respectively, and the lowest number is cysteine, with only 221 (1.105%) and 224 (1.096%), respectively. Protein collinearity analysis showed the high collinearity though several divergences were present in cp genomes, and identification of simple sequence repeats (SSRs) were included in this study. In addition, in order to compare cold tolerance and explore the expanding function of this environmental stress, the chlorophyll relative content (SPAD) and chlorophyll fluorescence Fv/Fm were measured. The significantly higher SPAD were observed in S. spontaneum than those in S. officinarum, no matter what the control conditions, exposure to low temperature or during recovery, and so was for Fv/Fm under exposure to low temperature, together with higher level of SPAD in S. spontaneum in each measurement. Aforementioned results suggest much stronger photosynthetic ability and cold tolerance in S. spontaneum. Our findings build a foundation to investigate the biological mechanism of two sugarcane ancestor chloroplasts and retrieve reliable molecular resources for phylogenetic and evolutionary studies, and will be conducive to genetic improvement of photosynthetic ability and cold resistance in modern sugarcane. [ABSTRACT FROM AUTHOR]

The plant-specific WRKY transcriptional regulatory factors have been proven to play vital roles in plant growth, development, and responses to biotic and abiotic stresses. However, there are few studies on the WRKY gene family in sugarcane (Saccharum spp.). In the present study, the characterization of a new subgroup, IIc WRKY protein ScWRKY3, from a Saccharum hybrid cultivar is reported. The ScWRKY3 protein was localized in the nucleus of Nicotiana benthamiana leaves and showed no transcriptional activation activity and no toxic effects on the yeast strain Y2HGold. An interaction between ScWRKY3 and a reported sugarcane protein ScWRKY4, was confirmed in the nucleus. The ScWRKY3 gene had the highest expression level in sugarcane stem pith. The transcript of ScWRKY3 was stable in the smut-resistant Saccharum hybrid cultivar Yacheng05-179, while it was down-regulated in the smut-susceptible Saccharum hybrid cultivar ROC22 during inoculation with the smut pathogen (Sporisorium scitamineum) at 0–72 h. ScWRKY3 was remarkably up-regulated by sodium chloride (NaCl), polyethylene glycol (PEG), and plant hormone abscisic acid (ABA), but it was down-regulated by salicylic acid (SA) and methyl jasmonate (MeJA). Moreover, transient overexpression of the ScWRKY3 gene in N. benthamiana indicated a negative regulation during challenges with the fungal pathogen Fusarium solani var. coeruleum or the bacterial pathogen Ralstonia solanacearum in N. benthamiana. The findings of the present study should accelerate future research on the identification and functional characterization of the WRKY family in sugarcane. [ABSTRACT FROM AUTHOR]

The purpose of this study is to examine whether for-profit firms make opportunistic use of their corporate foundations to pursue self-serving earnings objectives in China. Using data on corporate foundations and a sample of firms listed on the A-share market from 2010 to 2016, we first use the propensity score matching method to explore the effect of corporate foundations on earnings management of their founder firms. We find that the overall discretionary accruals of firms with corporate foundations are significantly higher than for those without corporate foundations. Given the ownership property with Chinese characteristics, we further find that the significant difference is driven by privately-owned firms. Then we develop a model of discretionary donation expenditures to measure the magnitude of earnings management associated with corporate foundations. We observe that firms with small profits and consecutive earnings increase record income-increasing discretionary donation expenditures. While firms that record income-decreasing discretionary donation expenditures create earnings reserves that they can use in subsequent periods to report consecutive earnings increases. The results demonstrate that the visibly ethical behavior of establishing corporate foundations does not necessarily represent the consistent embodiment of corporate social responsibility (CSR), but can be regarded as corporate hypocrisy with self-interest embedded in benevolence. [ABSTRACT FROM AUTHOR]

There is an urgent need to improve the corrosion resistance of WC-based cermet coatings in different corrosive environments. The main objective of this work was to investigate the microstructure and evaluate the corrosion resistance in neutral, acidic, and alkaline electrolytes of the WC-based cermet/Fe-based amorphous alloy composite coating. Thus, a composite coating of WC–CoCr/Fe-based amorphous alloy and a single WC–CoCr coating were fabricated using the high-velocity oxygen fuel (HVOF) process. The phase composition, microstructure of the original powders, and as-sprayed coatings were studied. The detailed interface information between different compositions of the composite coating was observed by high-resolution transmission electron microscopy (HRTEM). The corrosion resistance of the coatings was studied comparatively by electrochemical tests in 3.5 wt % NaCl, 1 M HCl and 1 M NaOH solutions, respectively. Results showed that the composited coating had a dense layered structure with a composition of WC, Fe-based amorphous alloy, and small amount of W2C. It was revealed that obvious inter-diffusion exists between the interfaces of tungsten carbide/Co, Cr binder and WC–CoCr/Fe-based amorphous alloy. The electrochemical test results showed that the composite coating displayed better corrosion resistance than single WC–CoCr coating both in 3.5 wt % NaCl solution and in 1 M NaOH solution. [ABSTRACT FROM AUTHOR]

Catalases, which consist of multiple structural isoforms, catalyze the decomposition of hydrogen peroxide in cells to prevent membrane lipid peroxidation. In this study, a group II catalase gene ScCAT2 (GenBank Accession No. KF528830) was isolated from sugarcane genotype Yacheng05-179. ScCAT2 encoded a predicted protein of 493 amino acid residues, including a catalase active site signature (FARERIPERVVHARGAS) and a heme-ligand signature (RVFAYADTQ). Subcellular localization experiments showed that the ScCAT2 protein was distributed in the cytoplasm, plasma membrane, and nucleus of Nicotiana benthamiana epidermal cells. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis indicated that the ScCAT2 gene was ubiquitously expressed in sugarcane tissues, with expression levels from high to low in stem skin, stem pith, roots, buds, and leaves. ScCAT2 mRNA expression was upregulated after treatment with abscisic acid (ABA), sodium chloride (NaCl), polyethylene glycol (PEG), and 4 °C low temperature, but downregulated by salicylic acid (SA), methyl jasmonate (MeJA), and copper chloride (CuCl2). Moreover, tolerance of Escherichia coli Rosetta cells carrying pET-32a-ScCAT2 was enhanced by NaCl stress, but not by CuCl2 stress. Sporisorium scitamineum infection of 10 different sugarcane genotypes showed that except for YZ03-258, FN40, and FN39, ScCAT2 transcript abundance in four smut-resistant cultivars (Yacheng05-179, YZ01-1413, YT96-86, and LC05-136) significantly increased at the early stage (1 day post-inoculation), and was decreased or did not change in the two smut-medium-susceptibility cultivars (ROC22 and GT02-467), and one smut-susceptible cultivar (YZ03-103) from 0 to 3 dpi. Meanwhile, the N. benthamiana leaves that transiently overexpressed ScCAT2 exhibited less severe disease symptoms, more intense 3,3′-diaminobenzidine (DAB) staining, and higher expression levels of tobacco immune-related marker genes than the control after inoculation with tobacco pathogen Ralstonia solanacearum or Fusarium solani var. coeruleum. These results indicate that ScCAT2 plays a positive role in immune responses during plant–pathogen interactions, as well as in salt, drought, and cold stresses. [ABSTRACT FROM AUTHOR]

Neurological diseases (NDs) are a leading cause of death worldwide and tend to mainly affect people under the age of 50. High rates of premature death and disability caused by NDs undoubtedly constrain societal development. However, effective therapeutic drugs and methods are very limited. Schisandra chinensis Fructus (SCF) is the dry ripe fruit of Schisandra chinensis (Turcz.) Baill, which has been used in traditional Chinese medicine for thousands of years. Recent research has indicated that SCF and its active ingredients show a protective role in NDs, including cerebrovascular diseases, neurodegenerative diseases, or depression. The key neuroprotective mechanisms of SCF and its active ingredients have been demonstrated to include antioxidation, suppression of apoptosis, anti-inflammation, regulation of neurotransmitters, and modulation of brain-derived neurotrophic factor (BDNF) related pathways. This paper summarizes studies of the role of SCF and its active ingredients in protecting against NDs, and highlights them as promising resources for future treatment. Furthermore, novel insights on the future challenges of SCF and its active ingredients are offered. [ABSTRACT FROM AUTHOR]

Sugarcane borer is the most common and harmful pest in Chinese sugarcane fields, and can cause damage to the whole plant during the entire growing season. To improve borer resistance in sugarcane, we constructed a plant expression vector pGcry2A0229 with the bar gene as the marker and the cry2A gene as the target, and introduced it into embryogenic calli of most widely cultivated sugarcane cultivar ROC22 by particle bombardment. After screening with phosphinothricin in vitro and Basta spray, 21 resistance-regenerated plants were obtained, and 10 positive transgenic lines harboring the cry2A gene were further confirmed by conventional PCR detection. Real-time quantitative PCR (RT-qPCR) analysis showed that the copy number of the cry2A gene varied among different transgenic lines but did not exceed four copies. Quantitative ELISA analysis showed that there was no linear relationship with copy number but negatively correlated with the percentage of borer-infested plants. The analysis of industrial and agronomic traits showed that the theoretical sugar yields of transgenic lines TR-4 and TR-10 were slightly lower than that of the control in both plant cane and ratoon cane; nevertheless, TR-4 and TR-10 lines exhibited markedly lower in frequency of borer-infested plants in plant cane and in the ratoon cane compared to the control. Our results indicate that the introduction of the cry2A gene via bombardment produces transgenic lines with obviously increased stem borer resistance and comparable sugar yield, providing a practical value in direct commercial cultivation and crossbreeding for ROC22 has been used as the most popular elite genitor in various breeding programs in China. [ABSTRACT FROM AUTHOR]

The leucine-rich repeat receptor-like protein kinase (LRR-RLK) gene family is the largest family of the receptor-like protein kinases (RLKs) superfamily in higher plants, which is involved in regulating the plant growth and development, stress responses, signal transduction and so on. However, no comprehensive analyses of LRR-RLKs have been reported in sugarcane. Here, we performed a comprehensive analysis of the LRR-RLK gene family in sugarcane ancestor species Saccharum spontaneum . A total of 437 LRR-RLK genes were identified and categorized into 14 groups based on a maximum likelihood phylogenetic tree. The chromosome location showed an uneven distribution on all 32 chromosomes in sugarcane. Subsequently, the exon-intron organization structure and conserved motif arrangement were relatively conserved among the same groups or subgroups and between Arabidopsis and S. spontaneum genomes. Furthermore, the promoter sequences analyses showed that sugarcane LRR-RLK genes ( SsLRR-RLKs ) were strongly regulated by various environmental stimuli, phytohormonal factors and transcription factors (TFs). Eventually, the expression profiles of SsLRR-RLK genes at different stresses were analyzed based on RNA-seq data, suggesting their potential roles in the regulation of sugarcane responses to diverse abiotic and biotic stress. Overall, the findings provide insight into the potential functional roles and lay the foundation for further functional study.

Mitogen-activated protein kinases (MPKs) play a central role not only in plant growth and development, but also in plant responses to abiotic and biotic stresses, including pathogens. Yet, their role in herbivore-induced plant defenses and their underlying mechanisms remain largely unknown. Here, we cloned a rice MPK gene, OsMPK4 , whose expression was induced by mechanical wounding, infestation of the striped stem borer (SSB) Chilo suppressalis , and treatment with jasmonic acid (JA), but not by treatment with salicylic acid (SA). The overexpression of OsMPK4 (oe-MPK4) enhanced constitutive and/or SSB-induced levels of JA, jasmonoyl-l-isoleucine (JA-Ile), ethylene (ET), and SA, as well as the activity of elicited trypsin proteinase inhibitors (TrypPIs), and reduced SSB performance. On the other hand, compared to wild-type plants, oe-MPK4 lines in the greenhouse showed growth retardation. These findings suggest that OsMPK4 , by regulating JA-, ET-, and SA-mediated signaling pathways, functions as a positive regulator of rice resistance to the SSB and a negative regulator of rice growth.

Transgene copy number has a great impact on the expression level and stability of exogenous gene in transgenic plants. Proper selection of endogenous reference genes is necessary for detection of genetic components in genetically modification (GM) crops by quantitative real-time PCR (qPCR) or by qualitative PCR approach, especially in sugarcane with polyploid and aneuploid genomic structure. qPCR technique has been widely accepted as an accurate, time-saving method on determination of copy numbers in transgenic plants and on detection of genetically modified plants to meet the regulatory and legislative requirement. In this study, to find a suitable endogenous reference gene and its real-time PCR assay for sugarcane (Saccharum spp. hybrids) DNA content quantification, we evaluated a set of potential "single copy" genes including P4H, APRT, ENOL, CYC, TST and PRR, through qualitative PCR and absolute quantitative PCR. Based on copy number comparisons among different sugarcane genotypes, including five S. officinarum, one S. spontaneum and two S. spp. hybrids, these endogenous genes fell into three groups: ENOL-3--high copy number group, TST-1 and PRR-1--medium copy number group, P4H-1, APRT-2 and CYC-2--low copy number group. Among these tested genes, P4H, APRT and CYC were the most stable, while ENOL and TST were the least stable across different sugarcane genotypes. Therefore, three primer pairs of P4H-3, APRT-2 and CYC-2 were then selected as the suitable reference gene primer pairs for sugarcane. The test of multi-target reference genes revealed that the APRT gene was a specific amplicon, suggesting this gene is the most suitable to be used as an endogenous reference target for sugarcane DNA content quantification. These results should be helpful for establishing accurate and reliable qualitative and quantitative PCR analysis of GM sugarcane.