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Simple Summary: Trichomonads are among the most prevalent intestinal parasites with a worldwide distribution which can infect many animals, resulting in economic losses and threatening public health. The donkey raising industry in Shanxi Province is relatively well-developed; however, it is not yet known whether donkeys in Shanxi Province were infected with Tetratrichomonas buttreyi and Pentatrichomonas hominis. Thus, 815 fecal samples were collected from donkeys in three representative geographical locations in Shanxi Province to determine the prevalence and associated risk factors of T. buttreyi and P. hominis in donkeys using molecular approaches. The overall prevalence of T. buttreyi and P. hominis in donkeys in Shanxi Province was 25.4% and 0.7%, respectively. Genetic analysis revealed that all P. hominis sequences obtained in this study were identified as genotype CC1, suggesting possible zoonotic potential. This is the first report of T. buttreyi and P. hominis prevalence in donkeys worldwide, which not only extends the geographical distribution of trichomonads but also expands the host spectrum. The findings also have implications for the prevention and control of trichomonad infections in donkeys in Shanxi Province. Two species of trichomonads, Tetratrichomonas buttreyi and Pentatrichomonas hominis, are common intestinal parasites that can impact animal health and productivity. Severe infection by these parasites can lead to diarrhea and wasting in affected animals. Notably, P. hominis is known to cause diarrhea and has the potential to be transmitted between animals and humans. Donkeys hold significant economic importance in China's agricultural sector. However, whether donkeys are infected with T. buttreyi and P. hominis remains unknown globally. To address this gap in knowledge, 815 fecal samples were collected from donkeys in three representative regions in Shanxi Province, North China. Then, the presence and genetic characteristics of T. buttreyi and P. hominis were examined using species-specific PCR primers amplifying the small subunit ribosomal RNA genes. The overall prevalence was detected to be 25.4% (207/815) for T. buttreyi and 0.7% (6/815) for P. hominis in donkeys in Shanxi Province. All obtained P. hominis sequences were identified as genotype CC1. Genetic analysis revealed that all P. hominis isolates from donkeys were clustered into the same branch with isolates detected in humans, suggesting possible zoonotic transmission. This study is the first to report the occurrence and prevalence of T. buttreyi and P. hominis in donkeys globally. These findings expand the host range of trichomonads and improve our understanding of their genetic diversity and zoonotic potential, providing essential baseline data for the prevention and control of these parasites in donkeys in the region. [ABSTRACT FROM AUTHOR]

Simple Summary: Blastocystis spp. and Giardia duodenalis are two prevalent intestinal parasites with a worldwide distribution that can infect humans and animals, resulting in significant public health concerns and economic losses. For goats, infection with the two parasites can cause symptoms such as diarrhea, which is not conducive to the development of stockbreeding. Shanxi Province is one of the largest goats breeding provinces in China. However, the prevalence of Blastocystis spp. and G. duodenalis in black goats in Shanxi Province remains unknown. Thus, 1200 fecal samples of black goats were collected in five representative geographical locations in Shanxi Province to examine the presence and genotypes of G. duodenalis and Blastocystis spp. by using a molecular approach. The results showed that the total infection rates of G. duodenalis and Blastocystis spp. were 7.5% and 3.5%, respectively. The presence and genotypes of G. duodenalis were determined based on three established loci (tpi, bg, and gdh). Among the detected assemblages B and E of G. duodenalis, the most prevalent assemblage was E in black goats in the five study areas. One novel MLG (MLG-E12) was identified by multilocus genotypes (MLGs) analysis. Through DNA sequence analysis, four subtypes of Blastocystis spp. were found in black goats, namely ST5, ST10, ST14, and ST30, among which ST10 was the dominant subtype in this study. This is the first report of Blastocystis spp. and G. duodenalis infection in black goats in Shanxi Province, which not only enhances our understanding of the genetic diversity of Blastocystis spp. and G. duodenalis in black goats in China but also provides essential baseline data for the prevention and control of Blastocystis spp. and G. duodenalis infection in black goats in the study areas. Blastocystis spp. and Giardia duodenalis are two prevalent zoonotic intestinal parasites that can cause severe diarrhea and intestinal diseases in humans and many animals. Black goat (Capra hircus) farming is increasingly important in China due to the remarkable adaptability, high reproductive performance, rapid growth rate, and significant economic value of black goats. A number of studies have indicated that black goats are the potential reservoir of multiple zoonotic protozoans in China; however, the prevalence and zoonotic status of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province is still unknown. Thus, a total of 1200 fecal samples of black goats were collected from several representative regions at different altitudes in Shanxi Province and were examined for the presence and genotypes of G. duodenallis and Blastocystis spp. by amplifying the beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) loci of G. duodenalis and SSU rRNA of Blastocystis spp. using PCR and sequence analysis methods, respectively. The overall prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province were 7.5% and 3.5%, respectively. Two assemblages (B and E) of G. duodenalis and four subtypes (ST5, ST10, ST14, and ST30) of Blastocystis spp. were identified, with assemblage E and ST10 as the prevalent genotype and subtype in black goats, respectively. One novel multilocus genotype (MLG) was identified in MLG-E and was designated as MLG-E12. For both G. duodenalis and Blastocystis spp., the prevalence was significantly related to the region and age groups (p < 0.05). This is the first report on the prevalence of G. duodenalis and Blastocystis spp. in black goats in Shanxi Province. These results not only provide baseline data for the prevention and control of both parasites in black goats in Shanxi Province, but also enhance our understanding of the genetic composition and zoonotic potential of these two parasites. [ABSTRACT FROM AUTHOR]

Simple Summary: Giardia duodenalis is a common zoonotic intestinal protozoan and causes significant economic losses and potential risks to public health. China is among the several major producers of donkeys worldwide, with approximately 2.6 million donkeys being reared in 2019, but studies on G. duodenalis infection in donkeys were limited. Thus, in order to better understand the molecular epidemiology of G. duodenalis in donkeys in China, 815 fecal samples were collected from donkeys in three representative geographical locations in Shanxi Province, North China, to determine the presence and genotypes of G. duodenalis based on three established loci (tpi, bg, and gdh). A total of 137 out of 815 fecal samples were detected as G. duodenalis-positive with an overall prevalence of 16.81%. Among the detected assemblages A, B, and E, the most predominant assemblage was B in the three study areas. Three MLGs (MLG-novel-1 to 3) were identified with multilocus genotypes (MLGs) analysis and validated by a phylogenetic tree. This is the first report of G. duodenalis infection in donkeys in Shanxi Province, which not only enhances our understanding of the genetic diversity of G. duodenalis in donkeys in China but also provides essential baseline data for the prevention and control of G. duodenalis infection donkeys in the study areas. Giardia duodenalis is a ubiquitous flagellated protozoan, causing significant economic losses to animal husbandry and posing threats to public health. China ranks the world's sixth largest major producer of donkeys, rearing approximately 2.6 million donkeys in 2019, but limited investigation of G. duodenalis prevalence has been conducted in the past, and it is yet to be known whether donkeys in Shanxi Province are infected with G. duodenalis. In the present study, a total of 815 fecal samples collected from donkeys in representative regions of Shanxi Province, North China, were examined for G. duodenalis using nested PCR. Then, the assemblages and multilocus genotypes (MLGs) were examined based on three established loci: namely, β-giardin (bg), triosephosphate isomerase (tpi), and glutamate dehydrogenase (gdh). The overall prevalence of G. duodenalis in donkeys in Shanxi Province was 16.81% (137/815). The region was identified as the main risk factor for the observed difference in G. duodenalis prevalence in donkeys among the three study areas (χ2 = 21.611, p < 0.001). Assemblages A, E, and B were identified, with the latter as the predominant assemblage. Three MLGs (MLG-novel-1 to 3) were formed based on sequence variation among the three loci. The present study reveals the presence of G. duodenalis in donkeys in Shanxi Province, North China, for the first time, which not only enriches the data on the distribution of G. duodenalis in donkeys in China but also provides useful baseline data for planning control strategies against G. duodenalis infection in the sampled areas. [ABSTRACT FROM AUTHOR]

Simple Summary: Toxoplasma gondii is a parasite that invades nucleated cells and causes changes in the cellular signal transduction network. This study aimed to perform a comprehensive analysis of the phosphorylated proteome in the small intestine cells of the definitive host following T. gondii infection. We used titanium dioxide affinity chromatography to enrich phosphopeptides in cat small intestinal epithelia infected with T. gondii and then used iTRAQ technology to quantify the phosphopeptides. A total of 4998 phosphopeptides, 3497 phosphorylation sites, and 1805 phosphoproteins were identified. Among the differentially expressed phosphoproteins, 68 were down-regulated and 637 were up-regulated. The bioinformatics analysis revealed that these phosphoproteins were involved in various cellular processes such as actin cytoskeleton reorganization, cell necroptosis, and the MHC immune process. The study confirmed that T. gondii infection leads to extensive changes in the phosphorylation of cat intestinal proteins. This is the first study to report global protein phosphorylation alterations in feline small intestinal epithelia following T. gondii infection. These findings provide a theoretical basis for understanding the interaction between T. gondii and its definitive host, which could have implications for the development of treatments or preventive measures. Toxoplasma gondii, an obligate intracellular parasite, has the ability to invade and proliferate within most nucleated cells. The invasion and destruction of host cells by T. gondii lead to significant changes in the cellular signal transduction network. One important post-translational modification (PTM) of proteins is phosphorylation/dephosphorylation, which plays a crucial role in cell signal transmission. In this study, we aimed to investigate how T. gondii regulates signal transduction in definitive host cells. We employed titanium dioxide (TiO2) affinity chromatography to enrich phosphopeptides in the small intestinal epithelia of cats at 10 days post-infection with the T. gondii Prugniuad (Pru) strain and quantified them using iTRAQ technology. A total of 4998 phosphopeptides, 3497 phosphorylation sites, and 1805 phosphoproteins were identified. Among the 705 differentially expressed phosphoproteins (DEPs), 68 were down-regulated and 637 were up-regulated. The bioinformatics analysis revealed that the DE phosphoproteins were involved in various cellular processes, including actin cytoskeleton reorganization, cell necroptosis, and MHC immune processes. Our findings confirm that T. gondii infection leads to extensive changes in the phosphorylation of proteins in the cat intestinal epithelial cells. The results of this study provide a theoretical foundation for understanding the interaction between T. gondii and its definitive host. [ABSTRACT FROM AUTHOR]

Simple Summary: Cryptosporidium spp. and Blastocystis sp. are zoonotic intestinal protozoa, which cause intestinal diseases in humans and animals. So far, the prevalence of these two parasites in cattle in Shanxi Province remains unknown. Thus, the present study collected 795 fecal samples from cattle in Shanxi Province and these fecal samples were examined for presence of Cryptosporidium spp. and Blastocystis sp. using molecular methods. The prevalence of Cryptosporidium spp. in cattle in Shanxi Province was 2.9% (23/795), and all of the positive samples were identified as C. andersoni. The prevalence of Blastocystis sp. in cattle in Shanxi Province was 13.0% (103/795), including five known subtypes (ST1, ST10, ST14, ST21 and ST26) and three unknown subtypes. Furthermore, the genetic diversity and distribution of Cryptosporidium spp. and Blastocystis sp. were further explored through the analysis of DNA sequences and phylogenetic trees. This study reveals the presence and prevalence of Cryptosporidium spp. and Blastocystis sp. in cattle in Shanxi Province for the first time, and provides baseline data for the control and prevention of both parasites in cattle in Shanxi Province. Both Cryptosporidium spp. and Blastocystis sp. are common intestinal protozoa, which can cause zoonotic diseases and economic losses to livestock industry. To evaluate the prevalence and genetic population structure of Cryptosporidium spp. and Blastocystis sp. in beef and dairy cattle in Shanxi Province, north China, a total of 795 fecal samples were collected from beef and dairy cattle in three representative counties in Shanxi Province, and these fecal samples were examined using molecular approaches based on 18S small-subunit ribosomal RNA (SSU rRNA) of Cryptosporidium spp. and Blastocystis sp., respectively. Among 795 cattle fecal samples, 23 were detected as Cryptosporidium-positive and 103 were detected as Blastocystis-positive, and the overall prevalence of Cryptosporidium spp. and Blastocystis sp. in cattle in Shanxi Province was 2.9% and 13.0%, respectively. For Cryptosporidium spp., DNA sequence analysis indicated that all 23 positive samples were identified as C. andersoni. Furthermore, five known subtypes (ST1, ST10, ST14, ST21 and ST26) and three unknown subtypes of Blastocystis sp. were detected among 103 positive samples using DNA sequence analysis. This study reported the occurrence and prevalence of Cryptosporidium spp. and Blastocystis sp. in cattle in Shanxi Province for the first time, which extends the geographical distribution of these two zoonotic parasites and provides baseline data for the prevention and control of these two important zoonotic parasites in cattle in Shanxi Province. [ABSTRACT FROM AUTHOR]

Simple Summary: Blastocystis is one of the most prevalent parasites, which can infect humans and many animal species worldwide, and the infection can result in public health problems and economic losses. Sheep and pigs are economically important animals in Shanxi province, north China; however, it is yet to be determined whether they are infected with Blastocystis. Thus, the present investigation was conducted to reveal the prevalence of Blastocystis in sheep and pigs in three representative counties in Shanxi province by examining 492 sheep feces and 362 pig feces using a molecular approach. The overall prevalence of Blastocystis in sheep and pigs were 16.26% and 14.09%, respectively. Five subtypes were found in sheep and pigs via DNA sequence analysis, of which ST5 was the dominant subtype in the three study counties. This study is the first to report the prevalence and subtypes of Blastocystis in sheep and pigs in Shanxi province. The findings not only extend the geographical distribution of Blastocystis but also provide baseline data for the prevention and control of Blastocystis infection in humans and animals in Shanxi province. Blastocystis is a common zoonotic intestinal protozoan and causes a series of gastrointestinal symptoms in humans and animals via the fecal–oral route, causing economic losses and posing public health problems. At present, the prevalence and genetic structure of Blastocystis in sheep and pigs in Shanxi province remains unknown. Thus, the present study collected 492 sheep fecal samples and 362 pig fecal samples from three representative counties in northern, central and southern Shanxi province for the detection of Blastocystis based on its SSU rRNA gene. The results showed that the overall prevalence of Blastocystis in the examined sheep and pigs were 16.26% and 14.09%, respectively. Sequences analyses showed that four known subtypes (ST5, ST10, ST14 and ST30) in sheep and two subtypes (ST1 and ST5) in pigs were detected in this study, with ST5 being the predominate subtype among the study areas. Phylogenetic analysis showed that the same subtypes were clustered into the same branch. This study reveals that sheep and pigs in Shanxi province are hosts for multiple Blastocystis subtypes, including the zoonotic subtypes (ST1 and ST5), posing a risk to public health. Baseline epidemiological data are provided that help in improving our understanding of the role of zoonotic subtypes in Blastocystis transmission. [ABSTRACT FROM AUTHOR]

Simple Summary: Toxocariasis is mainly caused by Toxocara canis, and to a lesser extent, Toxocara cati, and is a globally distributed zoonotic parasitic disease. Lipidomics is a discipline that has been developed after genomics and proteomics, and it is an important means of studying systems biology. In this study, we used liquid chromatography–tandem mass spectrometry (LC-MS/MS) to comprehensively examine the lipidomic alterations in the lungs of Beagle dogs infected with T. canis, and to analyze the differential lipids to reveal their potential biological functions. There were 63, 88, and 157 lipid species that changed significantly at 24 hpi, 96 hpi, and 36 dpi, respectively. Further analysis of the functions of these differential lipids, such as triglyceride (TG), lysophosphatidylserine (LPS), and ceramides (Cer), will better reveal the mechanism of T. canis pathogenesis. Toxocariasis, mainly caused by Toxocara canis, and to a lesser extent, Toxocara cati, is a neglected parasitic zoonosis. The mechanisms that underlie the changes in lipid metabolism of T. canis infection in Beagle dogs' lungs remain unclear. Lipidomics is a rapidly emerging approach that enables the global profiling of lipid composition by mass spectrometry. In this study, we performed a non-targeted lipidomic analysis of the lungs of Beagle dogs infected with the roundworm T. canis using liquid chromatography–tandem mass spectrometry (LC-MS/MS). A total of 1197 lipid species were identified, of which 63, 88, and 157 lipid species were significantly altered at 24 h post-infection (hpi), 96 hpi, and 36 days post-infection (dpi), respectively. This global lipidomic profiling identified infection-specific lipid signatures for lung toxocariasis, and represented a comprehensive comparison between the lipid composition of dogs' lungs in the presence and absence of T. canis infection. The potential roles of the identified lipid species in the pathogenesis of T. canis are discussed, which has important implications for better understanding the interaction mechanism between T. canis and the host lung. [ABSTRACT FROM AUTHOR]

Simple Summary: Enterocytozoon bieneusi is an important zoonotic intestinal protozoan that can infect humans and many types of animals. Currently, the prevalence and genotyping of E. bieneusi in beef cattle in China's Shanxi province is still unknown. Thus, nested PCR was applied in the present study to investigate the prevalence and genotypes of E. bieneusi by examining 401 fecal samples collected from beef cattle in two representative counties of Shanxi province. The results showed that the overall prevalence of E. bieneusi in beef cattle in Shanxi province is 22.44% (90/401). Six known genotypes (I, BEB4, J, BEB6, BEB8 and PigSpEb2) and two novel genotypes (designated CSC1 and CSC2) were identified, and genotype I was the predominant genotype in these two counties. Phylogenetic tree analysis indicated that five known genotypes and two novel genotypes were clustered into Group 2, whereas the genotype PigSpEb2 belongs to zoonotic Group 1. To our knowledge, the present study is the first to demonstrate the presence of E. bieneusi in beef cattle in Shanxi province, enriching the data on prevalence and genotypes of E. bieneusi in beef cattle and providing baseline data for executing intervention measures to control it in the study regions. Enterocytozoon bieneusi is an intracellular pathogen that can parasitize humans and a variety of animals. The infection of E. bieneusi in most hosts is asymptomatic, but in immunocompromised individuals, it can lead to serious complications such as acute diarrhea, dehydration, and even death. However, no data on the prevalence and genotyping of E. bieneusi in beef cattle in Shanxi province are currently available. In this study, a total of 401 fecal samples were collected from beef cattle in farms from two representative counties—Qi county and Jishan county—in Shanxi province, north China. Nested PCR was applied to determine the prevalence and genotypes of E. bieneusi by amplifying and sequencing the internal transcribed spacer (ITS) regions of the rRNA gene. A total of 90 out of 401 samples were detected as E. bieneusi-positive, with 22.44% overall prevalence of E. bieneusi in beef cattle in Shanxi province. The highest prevalence of E. bieneusi was detected in calves (28.67%, 41/143) and male beef cattle (28.13%, 54/192). Statistical analysis revealed that the prevalence of E. bieneusi was significantly associated with gender and age factors (p < 0.05), but without any statistical difference among regions. Moreover, six known E. bieneusi genotypes (BEB4, BEB6, BEB8, J, I, and PigSpEb2) and two novel genotypes (designated CSC1 and CSC2) were identified by analysis of ITS sequences, and genotype I was the predominant genotype in these two counties. Phylogenetic analysis showed that five known genotypes and two novel genotypes were clustered into Group 2, but PigSpEb2 belonged to Group 1. To our knowledge, the present study demonstrated the presence and identified genotypes of E. bieneusi in beef cattle in Shanxi province for the first time, extending the data on prevalence and genotypes of E. bieneusi in beef cattle and providing baseline data for executing intervention measures to control it in the study regions. [ABSTRACT FROM AUTHOR]

Simple Summary: As one of the neglected parasitic zoonoses, toxocariasis has gained increasing international attention. To date, however, little is known about the spleen microRNAs (miRNA) expression profiling in definitive or paratenic hosts after Toxocara canis infection. In this study, we conducted sequencing-based transcriptome profiling of miRNA expression in the spleen of Beagle puppies after T. canis infection to understand the potential biological roles of the altered miRNAs. Some differentially expressed miRNAs (DEmiRNAs) were identified after T. canis infection. The transcriptional profiling data suggested that the DEmiRNAs play significant roles in the pathogenesis of toxocariasis. Toxocara canis is an unnoticed zoonotic helminth that causes severe disease in animals and humans. The spleen has a wide range of immunological functions in protecting the host against infection by many pathogens, but the function of the spleen in T. canis infection is still to be clarified, especially for the role of spleen microRNAs (miRNAs). In this study, deep sequencing of spleen RNA samples of 18 Beagle puppies was conducted to uncover the miRNAs expression profiling at 24 h post-infection (hpi), 96 hpi, and 36 days post infection (dpi). A total of 20, 34, and 19 differentially expressed miRNAs (DEmiRNAs) were identified at 24 hpi, 96 hpi, and 36 dpi, respectively. These DEmiRNAs (e.g., cfa-miR-206, cfa-miR-331, and cfa-miR-339) could play critical roles in Beagle puppies against T. canis infection, such as influencing inflammatory and immune-related cells and cytokines, by regulating target genes that are tightly associated with host immune function and enriched in immune response and immune pathways based on GO annotation and KEGG enrichment analysis. The current study discovered marked alterations of spleen miRNAs after T. canis infection, with potential effects on the pathogenesis of toxocariasis. [ABSTRACT FROM AUTHOR]

Simple Summary: Toxocariasis is one of the most neglected zoonoses in the world. Toxocara canis is the main pathogen causing toxocariasis in humans and animals, threatening public health. To date, the mechanism by which the larvae of T. canis escape the attack of immune cells in the blood is still poorly understood. Using RNA-seq technology, the transcriptional alterations of Beagle dog peripheral blood mononuclear cells (PBMCs) between the presence and absence of T. canis infection were analyzed during the lung infection period, and 1066 upregulated genes and 1076 downregulated genes were identified (padj < 0.05 and |log2 (FoldChange)| > 1). In addition, many immune- or inflammation-related GO terms and KEGG signaling pathways were significantly altered during T. canis infection by GO annotation and KEGG enrichment analysis. The present study revealed that T. canis infection can alter the mRNA profiles of PBMCs in Beagle dogs during the lung infection period, which has important implications for a better understanding of the interaction mechanism between T. canis and host immune cells. Toxocara canis is a neglected zoonotic roundworm distributed all over the world, causing toxocariasis in humans and animals. However, so far, the immune mechanism of T. canis infection in definitive hosts remains to be clarified. In this study, the transcriptional alterations of Beagle dogs' peripheral blood mononuclear cells (PBMCs) induced by T. canis infection during the lung infection period were analyzed using RNA-seq technology. A total of 2142 differentially expressed genes were identified, with 1066 upregulated genes and 1076 downregulated genes. Many differentially expressed genes participated in the biological process of intracellular signal transduction, as well as the immune- or inflammation-related KEGG signaling pathway, such as the Notch signaling pathway, Toll-like receptor signaling pathway, and NF-kappa B signaling pathway, through KEGG enrichment analysis. This study indicated that T. canis infection could suppress the biological function of Beagle dogs' PMBCs and provided basic data to further clarify the interaction mechanism between T. canis and host immune cells. [ABSTRACT FROM AUTHOR]

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