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This paper models and analyzes the Complementary Metal Oxide Semiconductor (CMOS) cross-coupled differential-drive (CCDD) rectifier for Ultra-Low-Power ambient radio-frequency energy harvesters (RFEHs) working in the subthreshold region. In this paper, two closed-form equations of CCDD rectifier output voltage and input resistance in the subthreshold region were derived based on BSIM4 models of NMOS and PMOS. The model give insight to specify circuit parameters according to different inputs, transistor sizes, threshold voltages, numbers of stages, load conditions and compensation voltages, which can be used to optimize the rectifier circuit. There is a good agreement between the simulation results and these models, and these models have a maximum deviation of 10% in comparison with the simulation results in the subthreshold region. The measurement results of a single-stage CCDD rectifier reported in a previous paper were adopted to verify the model. The output voltage and input resistance predicted by these models provide excellent consistency with corresponding measurement results. The model can be employed to optimize the CCDD rectifier without expensive calculation in the design stage. [ABSTRACT FROM AUTHOR]

Green fiber (GF) is a naturally colored fiber. A limited understanding of its color formation mechanism restricts the improvement of colored cotton quality. This experiment used upland cotton green fiber germplasm 1-4560 and genetic inbred line TM-1; the lipid profiles of green fibers at 30 (white stage) and 35 days post-anthesis (DPA) (early greening stage), as well as those of TM-1 at the same stages, were revealed. Among the 109 differential types of lipids (DTLs) unique to GF, the content of phosphatidylserine PS (16:0_18:3) was significantly different at 30 and 35 DPA. It is speculated that this lipid is crucial for the pigment accumulation and color formation process of green fibers. The 197 DTLs unique to TM-1 may be involved in white fiber (WF) development. Among the shared DTLs in GF35 vs. GF30 and WF35 vs. WF30, sulfoquinovosyldiacyl-glycerol SQDG (18:1_18:1) displays a significant difference in the content change between green fibers and white fibers, potentially affecting color formation through changes in content. The enriched metabolic pathways in both comparison groups are relatively conserved. In the most significantly enriched glycerophospholipid metabolic pathway, 1-acyl-sn-glycero-3-phosphocholine (C04230) only appears in white cotton. This indicates differences in the metabolic pathways between white and green fibers, potentially related to different mechanisms of color formation and fiber development. These findings provide a new theoretical basis for studying cotton fiber development and offer important insights into the specific mechanism of green fiber color formation. [ABSTRACT FROM AUTHOR]

Atmospheric gravity waves (GWs) can strongly modulate middle atmospheric circulation and can be a significant factor for the coupling between the lower atmosphere and the middle atmosphere. GWs are difficult to resolve in global atmospheric models due to their small scale; thus, GW observations play an important role in middle atmospheric studies. The climatology of GW variance and momentum in the low-latitude mesosphere and lower thermosphere (MLT) region are revealed using multiple meteor radars, which are located at Kunming (25.6°N, 103.8°E), Sanya (18.4°N, 109.6°E), and Fuke (19.5°N, 109.1°E). The climatology and longitudinal variations in GW momentum fluxes and variance over the low-latitude region are reported. The GWs show strong seasonal variations and can greatly control the mesospheric horizontal winds via modulation of the quasi-geostrophic balance and momentum deposition. The different GW activities between Kunming and Sanya/Fuke are possibly consistent with the unique prevailing surface winds over Kunming and the convective system over the Tibetan Plateau according to the European Centre for Medium-Range Weather Forecasts (ECMWF), Reanalysis v5 (ERA5) data, and outgoing longwave radiation (OLR) data. These findings provide insight for better understanding the coupling between the troposphere and mesosphere. [ABSTRACT FROM AUTHOR]

Ordos City is an important energy supply city for Chinese provinces and cities, providing a secure energy supply for China while also generating corresponding environmental pollution. Examining the spatiotemporal patterns of net primary productivity (NPP) in Ordos City and its driving factors is relevant to the realization of the carbon emission policy in Inner Mongolia. This study was undertaken to analyze NPP and its driving factors in Ordos City from 2000 to 2019 using NPP data, CO2 spatial grid data, meteorological data and statistical yearbook data accordingly. The NPP in Ordos City increased significantly from 2000 to 2019, mainly showing low values of NPP in the northwest and high values in the southeast. The usable grassland area and annual mean precipitation had a significant positive correlation with NPP, whereas the other factors had a more significant negative correlation. The usable grassland area had the largest influence on NPP, and fixed asset investment had the smallest influence on NPP. The total NPP–anthropogenic factor regression model and the mean NPP–natural factor regression model constructed allow for the prediction of NPP. Anthropogenic carbon emissions, population growth and usable grassland area were the main causes of NPP changes. Planting and protecting green plants and scientific and effective energy extraction plans are measures that enhance the degree of carbon sequestration in Ordos City. [ABSTRACT FROM AUTHOR]

The intraseasonal oscillations (30–100 days, ISO) in the MLT (mesosphere and lower thermosphere) horizontal wind are investigated based on observations from the Mengcheng meteor radar. There is a clear seasonal variation in ISO in the horizontal wind at 80 km, which is strongest during the winter and weakest during the summer. At 100 km, ISO occurs throughout most of the year except winter, and there are significant differences in periods and amplitudes from year to year. From 2015 to 2016, ISOs with periods of 40–60 days were present in the 100 km horizontal wind, whereas none were simultaneously observed in the 80 km horizontal wind. Cross wavelets were used to study the relationship between ISO in the MLT region and ISO in the lower atmosphere. Some of the ISO activity is linked to tropospheric tropical convective activity, but the ISO connections with that in tropospheric convection are not consistent in the upper mesosphere and in the lower thermosphere. [ABSTRACT FROM AUTHOR]

This study presents the observations of midlatitude plasma irregularities over Eastern Asia during a moderate magnetic storm on 16 July 2003. Multi-instrumental observations, including the ground-based ionosondes, the GNSS networks, and the CHAMP and ROCSAT-1 satellites, were utilized to investigate the occurrence and characteristics of midlatitude plasma irregularities. The midlatitude strong spread F (SSF) mainly occurred in the midnight–morning sector as observed by ionosondes over Japan during this storm. SSF was related to plasma depletions, which is also recorded by GNSS network in the form of the enhancement of the rate of total electron content (TEC) change index (ROTI). The possible mechanism for the generation of SSF is that the enhanced eastward electric fields, associated with the prompt penetration electric fields and disturbance dynamo electric fields, cause the uplift and latitudinal extension of equatorial plasma bubbles (EPBs) to generate the observed midlatitude SSF further. Meanwhile, plasma density increased significantly under the influence of this storm. In addition, other common type of spread F, frequency spread F (FSF), was observed over Japan on the non-storm day and/or at high latitude station WK545, which seems to be closely related to the coupling of medium-scale traveling ionospheric disturbances (MSTIDs) and sporadic E (Es) layer. The above results indicate that various types of midlatitude spread F can be produced by different physical mechanisms. It is found that SSF can significantly affect the performance of radio wave propagation compared with FSF. Our results show that space weather events have a significant influence on the day-to-day variability of the occurrence and characteristics of ionospheric F-region irregularities at midlatitudes. [ABSTRACT FROM AUTHOR]

In the past few years, the assessment of Parkinson's disease (PD) has mainly been based on the clinician's examination, the patient's medical history, and self-report. Parkinson's disease may be misdiagnosed due to a lack of clinical experience. Moreover, it is highly subjective and is not conducive to reflecting a true result. Due to the high incidence rate and increasing trend of PD, it is significant to use objective monitoring and diagnostic tools for accurate and timely diagnosis. In this paper, we designed a low-level feature extractor that uses convolutional layers to extract local information about an image and a high-level feature extractor that extracts global information about an image through the autofocus mechanism. PD is detected by fusing local and global information. The model is trained and evaluated on two publicly available datasets. Experiments have shown that our model has a strong advantage in diagnosing whether people have PD; gait-based analysis and recognition can also provide effective evidence for the early diagnosis of PD. [ABSTRACT FROM AUTHOR]

In this study, we present nearly 9 years of the quasi16-day wave (Q16DW) in the mesosphere and lower thermosphere (MLT) wind at middle latitudes based on long-term wind observations between April 2014 and December 2022 by the Mengcheng (33.4°N, 116.5°E) meteor radar. There are two maxima in the Q16DW amplitude in the winter and early spring (near the equinox) and a minimum during the summer. The Q16DWs are relatively weak in meridional winds with no obvious seasonal variations. On average, the phase of the zonal Q16DW is larger than the meridional components with a mean difference that is slightly less than 90°, which suggests that there are orthogonal relationships between them. During the bursts of Q16DW, the periods in winter range between 15 and 18 d, whereas in summer, the periods of the planetary waves have a wider range. The wintertime Q16DW anomalies are, on average, amplified when the zonal wind shear anomalies increase, suggesting that barotropic instability may be a source of the Q16DW. Although the interannual variability of Q16DW amplitudes has been suggested observationally, there is no significant relationship between the interannual wind shear variability and Q16DW at most altitudes. [ABSTRACT FROM AUTHOR]

With the purpose of radiation emissions and immunity measurement of large size and high-frequency integrated circuits (ICs), three closed version IC-Stripline cells conforming to the IEC standardswere designed in this paper to expand the uniform field region and the working frequency band of the traditional IC-Stripline cell. In these cells, the long version IC-Stripline cell expands the 3 dB uniform field region of the traditional IC-Stripline cell from 3 × 3 cm to 3 × 8 cm, with the working bandwidth remaining as 6 GHz. The IC-Stripline cell based on image theory further expands the 3 dB uniform field region into two 3 × 8 cm areas with the working bandwidth unchanged. The third cell is a scaled-down IC-Stripline cell based on image theory, which can expand the working bandwidth from 6 GHz to 12 GHz and the total area of the uniform field region is equivalent to that of the traditional IC-Stripline cell. [ABSTRACT FROM AUTHOR]

Aortic dissection (AD) is a life-threatening disease with high morbidity and mortality, and effective pharmacotherapeutic remedies for it are lacking. Therefore, AD's molecular pathogenesis and etiology must be elucidated. The aim of this study was to investigate the possible mechanism of mediator complex subunit 12 (human: MED12, mouse: Med12)involvement in AD. Firstly, we examined the expression of MED12 protein (human: MED12, mouse: Med12) in the aortic tissues of AD patients and AD mice. Subsequently, Med12 gene silencing was accomplished with RNA interference (siRNA). The effects of Med12 on AD and the possible biological mechanisms were investigated based on the proliferation, senescence, phenotypic transformation, and its involved signal pathway of mouse aortic smooth muscle cells (MOVAS), s. The results show that the expression of MED12 in the aortae of AD patients and AD mice was decreased. Moreover, the downregulation of Med12 inhibited the proliferation of MOVAS and promoted senescence. Further research found that Med12, as an inhibitor of the TGFβ1 signaling pathway, reduced the expression of Med12 and enhanced the activity of the TGFβ1 nonclassical signaling pathway, while TGFβ1 inhibited the phenotype transformation and proliferation of MOVAS by inhibiting Med12 synthesis. In conclusion, Med12 affected the phenotype, proliferation, and senescence of MOVAS through the TGFβ signaling pathway. This study provides a potential new target for the prevention and treatment of AD. [ABSTRACT FROM AUTHOR]

Auxin has a profound impact on plant physiology and participates in almost all aspects of plant development processes. Auxin exerts profound pleiotropic effects on plant growth and differentiation by regulating the auxin response genes' expressions. The classical auxin reaction is usually mediated by auxin response factors (ARFs), which bind to the auxin response element (AuxRE) in the promoter region of the target gene. Experiments have generated only a limited number of plant genes with well-characterized functions. It is still unknown how many genes respond to exogenous auxin treatment. An economical and effective method was proposed for the genome-wide discovery of genes responsive to auxin in a model plant, Arabidopsis thaliana (A. thaliana). Our method relies on cis-regulatory-element-based targeted gene finding across different promoters in a genome. We first exploit and analyze auxin-specific cis-regulatory elements for the transcription of the target genes, and then identify putative auxin responsive genes whose promoters contain the elements in the collection of over 25,800 promoters in the A. thaliana genome. Evaluating our result by comparing with a published database and the literature, we found that this method has an accuracy rate of 65.2% (309/474) for predicting candidate genes responsive to auxin. Chromosome distribution and annotation of the putative auxin-responsive genes predicted here were also mined. The results can markedly decrease the number of identified but merely potential auxin target genes and also provide useful clues for improving the annotation of gene that lack functional information. [ABSTRACT FROM AUTHOR]

The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties. [ABSTRACT FROM AUTHOR]

As a notable illustration of totipotency and plant regeneration, somatic embryogenesis (SE) is the developmental reprogramming of somatic cells toward the embryogenesis pathway, the key step for genetic engineering. Investigations examining the totipotency process are of great fundamental and practical importance in crop biotechnology. However, high-frequency regeneration of cotton via SE has been limited due to genotype-dependent response. The molecular basis deciphering SE genotype recalcitrance remains largely unexplored in cotton. In the current study, to comprehensively investigate the dynamic transcriptional profiling and gene regulatory patterns involved in SE process, a genome-wide RNA sequencing analysis was performed in two cotton genotypes with distinct embryogenic abilities, the highly embryogenic genotype Yuzao 1 (YZ) and the recalcitrant genotype Lumian 1 (LM). Three typical developmental staged cultures of early SE—hypocotyls (HY), nonembryogenic calli (NEC) and primary embryogenic calli (PEC)—were selected to establish the transcriptional profiles. Our data revealed that a total of 62,562 transcripts were present amongst different developmental stages in the two genotypes. Of these, 18,394 and 26,514 differentially expressed genes (DEGs) were identified during callus dedifferentiation (NEC-VS-HY) and embryogenic transdifferentiation (PEC-VS-NEC), respectively in the recalcitrant genotype, 21,842 and 22,343 DEGs in the highly embryogenic genotype. Furthermore, DEGs were clustered into six expression patterns during cotton SE process in the two genotypes. Moreover, functional enrichment analysis revealed that DEGs were significantly enriched in fatty acid, tryptophan and pyruvate metabolism in the highly embryogenic genotype and in DNA conformation change otherwise in the recalcitrant genotype. In addition, critical SE-associated expressed transcription factors, as well as alternative splicing events, were notably and preferentially activated during embryogenic transdifferentiation in the highly embryogenic genotype compared with the recalcitrant genotype. Taken together, by systematically comparing two genotypes with distinct embryogenic abilities, the findings in our study revealed a comprehensive overview of the dynamic gene regulatory patterns and uncharacterized complex regulatory pathways during cotton SE genotype-dependent response. Our work provides insights into the molecular basis and important gene resources for understanding the underlying genotype recalcitrance during SE process and plant regeneration, thereby holding great promise for accelerating the application of biotechnology to cotton for improving its breeding efficiency. [ABSTRACT FROM AUTHOR]

Developing cheap, clean and atomic-efficient synthetic methodologies for conjugated polymers are always critical for the field of organic electronics. Herein, classic Ullmann coupling polymerization is developed to synthesize a series of Acceptor-Acceptor (A-A) type homopolymers with microwave-assistance, which are supported by nuclear magnetic resonance (NMR), matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF), elemental analysis (EA) and gel permeation chromatography (GPC). The physicochemical properties of these polymers are studied by UV-vis spectroscopy, cyclic voltammetry (CV), thermal gravimetric analysis (TGA), and density functional theory (DFT) calculation. Furthermore, these A-A homopolymers are used as acceptors for all-polymer solar cells (All-PSCs), affording a promising efficiency of 3.08%, which is the highest value for A-A-homopolymer-based organic solar cells. [ABSTRACT FROM AUTHOR]

Plant regeneration via somatic embryogenesis (SE) is a key step during genetic engineering. In the current study, integrated widely targeted metabolomics and RNA sequencing were performed to investigate the dynamic metabolic and transcriptional profiling of cotton SE. Our data revealed that a total of 581 metabolites were present in nonembryogenic staged calli (NEC), primary embryogenic calli (PEC), and initiation staged globular embryos (GE). Of the differentially accumulated metabolites (DAMs), nucleotides, and lipids were specifically accumulated during embryogenic differentiation, whereas flavones and hydroxycinnamoyl derivatives were accumulated during somatic embryo development. Additionally, metabolites related to purine metabolism were significantly enriched in PEC vs. NEC, whereas in GE vs. PEC, DAMs were remarkably associated with flavonoid biosynthesis. An association analysis of the metabolome and transcriptome data indicated that purine metabolism and flavonoid biosynthesis were co-mapped based on the Kyoto encyclopedia of genes and genomes (KEGG) database. Moreover, purine metabolism-related genes associated with signal recognition, transcription, stress, and lipid binding were significantly upregulated. Moreover, several classic somatic embryogenesis (SE) genes were highly correlated with their corresponding metabolites that were involved in purine metabolism and flavonoid biosynthesis. The current study identified a series of potential metabolites and corresponding genes responsible for SE transdifferentiation, which provides a valuable foundation for a deeper understanding of the regulatory mechanisms underlying cell totipotency at the molecular and biochemical levels. [ABSTRACT FROM AUTHOR]

Auxin has a profound impact on plant physiology and participates in almost all aspects of plant development processes. Auxin exerts profound pleiotropic effects on plant growth and differentiation by regulating the auxin response genes' expressions. The classical auxin reaction is usually mediated by auxin response factors (ARFs), which bind to the auxin response element (AuxRE) in the promoter region of the target gene. Experiments have generated only a limited number of plant genes with well-characterized functions. It is still unknown how many genes respond to exogenous auxin treatment. An economical and effective method was proposed for the genome-wide discovery of genes responsive to auxin in a model plant, Arabidopsis thaliana ( A. thaliana ). Our method relies on cis -regulatory-element-based targeted gene finding across different promoters in a genome. We first exploit and analyze auxin-specific cis -regulatory elements for the transcription of the target genes, and then identify putative auxin responsive genes whose promoters contain the elements in the collection of over 25,800 promoters in the A. thaliana genome. Evaluating our result by comparing with a published database and the literature, we found that this method has an accuracy rate of 65.2% (309/474) for predicting candidate genes responsive to auxin. Chromosome distribution and annotation of the putative auxin-responsive genes predicted here were also mined. The results can markedly decrease the number of identified but merely potential auxin target genes and also provide useful clues for improving the annotation of gene that lack functional information.