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Anopheles stephensi (Diptera: Culicidae) is the vector of urban malaria in India and has a significant impact in transmitting infection in cities and towns. Further, WHO has also alarmed its invasive nature as a threat to African countries. Entomopathogenic fungi such as Beauveria bassiana and Metarhizium anisopliae have been found to be highly effective in controlling vector mosquito populations and therefore could be used in integrated vector control programs. Before employing the entomopathogenic fungi into the control programs, an effective isolate must be selected. Two separate experiments were conducted to evaluate the efficacy of Beauveria bassiana (Bb5a and Bb-NBAIR) and Metarhizium anisopliae (Ma4 and Ma-NBAIR) isolates against An. stephensi. Cement and mud panels were treated with fungal conidia with the concentration of 1 × 107 conidia/mL and adult An. stephensi mosquitoes were exposed to the treated panels (24 h after conidia were applied) by conducting WHO cone bioassay tests. The survival of the mosquitoes was monitored daily until the 10th day. In the second experiment, second instar larvae of An. stephensi were treated with fungal (Bb5a, Bb-NBAIR, Ma4 and Ma-NBAIR) conidia and blastospores with the spore concentration of 1 × 107 spores/mL. The survival of larvae was monitored until pupation. All the fungal isolates tested caused mortality in the adult mosquitoes, with varying median survival times. The Bb5a isolate reported lesser median survival times on both cement and mud panels (6 days). The treated mosquitoes showed similar survival rates for each fungal isolate irrespective of the panel type. There was no mortality in the treated larvae; however, a delay in larval development to pupae was observed compared with the untreated control larvae. Ma4-treated larvae took 11 days (95% CI = 10.7–11.2) to become pupae when compared with the untreated control larvae (6 days [95% CI = 5.6–6.3]). The findings of this study will be useful to consider EPF as a tool for the management of vector mosquitoes. [ABSTRACT FROM AUTHOR]