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Background We aimed to evaluate the association of serum C-reactive protein (CRP) with prognosis in patients with locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy. Methods We retrospectively reviewed 79 patients with locoregionally advanced nasopharyngeal carcinoma (cT3–4N0–3M0) treated with chemoradiotherapy. Chemoradiotherapy consisted of external-beam radiotherapy to the nasopharynx (70–80 Gy), the lymph node-positive area (60–70 Gy), and the lymph node–negative area (50–60 Gy) combined with 3 cycles of various platinum-based regimens delivered at 3-week intervals. Elevated CRP was defined as more than 8 mg/L. The survival rate was calculated using the Kaplan-Meier method, and univariate and multivariate analyses (Cox proportional hazards model) were used to identify factors significantly associated with prognosis. Results During the median follow-up of 3.9 years (range: 1–5.5 years), 23 patients died from nasopharyngeal cancer. The 5-year cancer-specific survival (CSS) rate was 62.90%. Before chemoradiotherapy, 18 patients had high serum CRP; the CSS rate in that subgroup was significantly worse than the rate in the remaining patients (p = 0.0002). Multivariate analysis showed that CRP was an independent prognostic indicator of CSS, with a hazard ratio of 3.04 (95% confidence interval: 1.22 to 7.55; p = 0.017). Among the 18 patients with elevated serum CRP, 9 achieved normal serum CRP after chemoradiotherapy, of whom 5 remained living with no evidence of recurrence or metastasis during follow-up. By contrast, the remaining 9 patients in whom serum CRP did not normalize after chemoradiotherapy died within 4.2 years. Conclusions Elevated serum CRP before treatment predicts poor prognosis in patients with locoregionally advanced nasopharyngeal carcinoma treated with chemoradiotherapy. [ABSTRACT FROM AUTHOR]

Mesocotyl plays a key role in the seedling emergence of maize; however, the mechanism of mesocotyl elongation is still unclear. Moreover, different maize inbred lines and cultivars have varied mesocotyl lengths positively correlated with deep sowing tolerance. In this study, we selected one inbred line with long mesocotyl (LM) and two maize inbred lines with short mesocotyl (SM1 and SM2) from more than 400 maize inbred lines. The mesocotyl length of the LM line was about three-fold longer than those of the SM1 and SM2 lines. Microstructure observation showed that the reason for short mesocotyl in the SM1 and SM2 lines was few cell numbers and short cell length, respectively. Subsequently, we used RNA-seq to investigate the mechanism of mesocotyl elongation by regulating cell number and cell length at the transcriptome level. Compared with the LM line, the SM1 line displayed stronger downregulation of Cytochrome P450 and peroxidase genes than the SM2 line. Moreover, plant hormone signal transduction plays a vital role in mesocotyl elongation. Taken together, we propose a model for mesocotyl elongation of maize inbred lines with different cell lengths and cell numbers, which provide new insights into mesocotyl elongation in maize.

As one of the most critical components in lithium-ion batteries (LIBs), commercial polyolefin separators suffer from drawbacks such as poor thermal stability and the inability to inhibit the growth of dendrites, which seriously threaten the safety of LIBs. In this study, we prepared calcium alginate fiber/boron nitride-compliant separators (CA@BN) through paper-making technology and the surface coating method using calcium alginate fiber and boron nitride. The CA@BN had favorable electrolyte wettability, flame retardancy, and thermal dimensional stability of the biomass fiber separator. Meanwhile, the boron nitride coating provided excellent thermal conductivity and mechanical strength for the composite separator, which inhibited the growth of lithium dendrites and enabled lithium-ion symmetric batteries to achieve more than 1000 stable and long cycles at a current density of 0.5 mA cm -2 . The interwoven fiber mesh formed by the boron nitride coating and the calcium alginate provided multiple pathways for ion migration, which enhanced the storage capacity of the electrolyte, improved the interfacial compatibility between the separator and the electrode, widened the window of electrochemical stability, and enhanced ionic migration. This eco-friendly bio-based separator paves a new insight for the design of heat-resistance separators as well as the safe running of LIBs.

The aim of this study was to explore the effects of temperature and light on microalgal growth and nutrient removal in turtle aquaculture wastewater using a single-factor experiment method. Results showed that the growth process of Desmodesmus sp. CHX1 in turtle aquaculture wastewater exhibited three stages, namely adaptation, logarithmic, and stable periods. Temperature and light significantly influenced the growth and protein and lipid accumulation of Desmodesmus sp. CHX1. The optimal conditions for the growth and biomass accumulation of Desmodesmus sp. CHX1 included a temperature of 30 °C, a photoperiod of 24L:0D, and a light intensity of 180 μmol photon/(m 2 ·s). Increased temperature, photoperiod, and light intensity enhanced nutrient removal efficiency. Maximum nitrogen removal was achieved at a temperature of 30 °C, a photoperiod of 24L:0D, and a light intensity of 180 μmol photon/(m 2 ·s), with the removal efficiency of 86.53%, 97.94%, 99.57%, and 99.15% for ammonia, nitrate, nitrite, and total phosphorus (TP), respectively. Temperature did not significantly affect TP removal, but increased photoperiod and light intensity improved the removal efficiency of TP. The development of microalgae biomass as a feed rich in protein and lipids could address feed shortages and meet the nutritional needs of turtles, offering a feasible solution for large-scale production.

Metal ion intercalation into van der Waals gaps of layered materials is vital for large-scale electrochemical energy storage. Transition-metal sulfides, ABS 4 (where A and B represent Zr, Hf, and Ti as monolayers as anodes), are examined as lithium and sodium ion storage. Our study reveals that these monolayers offer exceptional performance for ion storage. The low diffusion barriers enable efficient lithium bonding and rapid separation while all ABS 4 phases remain semiconducting before lithiation and transition to metallic states, ensuring excellent electrical conductivity. Notably, the monolayers demonstrate impressive ion capacities: 1639, 1202, and 1119 mAh/g for Li-ions, and 1093, 801, and 671 mAh/g for Na-ions in ZrTiS 4 , HfTiS 4 , and HfZrS 4 , respectively. Average voltages are 1.16 V, 0.9 V, and 0.94 V for Li-ions and 1.17 V, 1.02 V, and 0.94 V for Na-ions across these materials. Additionally, low migration energy barriers of 0.231 eV, 0.233 eV, and 0.238 eV for Li and 0.135 eV, 0.136 eV, and 0.147 eV for Na make ABS 4 monolayers highly attractive for battery applications. These findings underscore the potential of monolayer ABS 4 as a superior electrode material, combining high adsorption energy, low diffusion barriers, low voltage, high specific capacity, and outstanding electrical conductivity.

Background/Objectives : The emergence of resistance to Staphylococcus hyicus ( S. hyicus ), the major cause of exudative epidermatitis (EE) in piglets, has led to the need for new antimicrobial agents. The study aimed to evaluate the potential efficacy of the insect defensin DLP4 against EE in piglets caused by clinically isolated S. hyicus ACCC 61734. Methods and Results : DLP4 showed strong antibacterial activity against S. hyicus ACCC 61734 (minimum inhibitory concentration, MIC: 0.92 μM, median effect concentration, EC 50 : 3.158 μM). DLP4 could effectively inhibit the formation of S. hyicus early biofilm with an inhibition rate of 95.10-98.34% and eradicate mature biofilm with a clearance rate of 82.09-86.41%, which was significantly superior to that of ceftriaxone sodium (CRO). Meanwhile, DLP4 could efficiently inhibit bacteria in early and mature biofilm, killing up to 95.3% of bacteria in early biofilm and 87.2-90.3% of bacteria in mature biofilm. The results showed that DLP4 could be effective in alleviating the clinical symptoms of EE by down-regulating the nuclear factor κB (NF-κB) signaling pathway, balancing cytokines, inhibiting bacterial proliferation, and reducing organ tissue damage. Conclusions : This study firstly demonstrated the potential efficacy of DLP4 against EE caused by S. hyicus ACCC 61734 infection in piglets, which may be used as an alternative to antibiotics in treating EE.

This work proposes a methodology to determine the height difference of the guideways of machine tools where two guideways are not placed on the same horizontal plane. Firstly, a rigid-flexible coupling system consisting of a moving rigid mass and an elastic overhanging beam is presented as an equivalent mechanical model of a column and a spindle box. Then, the relationship between the deviation of a reference point and the height difference or the spindle box's stroke is modeled. Next, the natural frequency and mode shape function of the overhanging beam, and the frequency response functions of the coupling system, are derived. The results indicate that there always exists an optimal height difference minimizing the relative deflection over the stroke of the moving part, and the optimal value depends on the loads in two directions and the stroke of the moving part. Similarly, there is also an optimal choice maximizing the first-order resonant frequency of the coupling system; however, the optimal solutions for both static and dynamic cases are not the same. This work provides beneficial instruction for choosing the height difference of machine tools with two guideways on a bed that are not on the same plane.

Breast cancer, the most prevalent malignant tumor among women globally, remains a critical area of focus for researchers striving to refine therapeutic approaches. As an important component of traditional Chinese medicine, Astragalus membranaceus (AM) has demonstrated potential for multifaceted impacts on breast cancer treatment through various mechanisms. To guide clinical practice and further explore the under-researched field of AM in breast cancer treatment, this paper mainly reviews the regulatory roles of AM-derived compounds and extracts on breast cancer cell proliferation, migration, invasion, and chemoresistance. Furthermore, this study delves into the synergistic effects observed when AM is co-administered with chemotherapeutic agents, including the enhancement of chemosensitivity, mitigation of toxic side effects, and reversal of drug resistance. This review indicates that AM holds promise not only as a therapy in breast cancer treatment but also paves the way for innovative integrated treatment approaches that combine the benefits of traditional medicine with modern pharmaceuticals. Nevertheless, future research endeavors are also urged to elucidate the in vivo pharmacological effects and underlying mechanisms of AM to inform more effective clinical treatment strategies.

High-resolution remote sensing imagery, reaching meter or sub-meter levels, provides essential data for extracting and identifying road information. However, rural roads are often narrow, elongated, and have blurred boundaries, with textures that resemble surrounding environments such as construction sites, vegetation, and farmland. These features often lead to incomplete extraction and low extraction accuracy of rural roads. To address these challenges, this study introduces the RC-MSFNet model, based on the U-Net architecture, to enhance rural road extraction performance. The RC-MSFNet model mitigates the vanishing gradient problem in deep networks by incorporating residual neural networks in the downsampling stage. In the upsampling stage, a connectivity attention mechanism is added after dual convolution layers to improve the model's ability to capture road completeness and connectivity. Additionally, the bottleneck section replaces the traditional dual convolution layers with a multi-scale fusion atrous convolution module to capture features at various scales. The study focuses on rural roads in the Xiong'an New Area, China, using high-resolution imagery from China's Gaofen-2 satellite to construct the XARoads rural road dataset. Roads were extracted from the XARoads dataset and DeepGlobe public dataset using the RC-MSFNet model and compared with some models such as U-Net, FCN, SegNet, DeeplabV3+, R-Net, and RC-Net. Experimental results showed that: (1) The proposed method achieved precision (P), intersection over union (IOU), and completeness (COM) scores of 0.8350, 0.6523, and 0.7489, respectively, for rural road extraction in Xiong'an New Area, representing precision improvements of 3.8%, 6.78%, 7.85%, 2.14%, 0.58%, and 2.53% over U-Net, FCN, SegNet, DeeplabV3+, R-Net, and RC-Net. (2) The method excelled at extracting narrow roads and muddy roads with unclear boundaries, with fewer instances of omission or false extraction, demonstrating advantages in complex rural terrain and areas with indistinct road boundaries. Accurate rural road extraction can provide valuable reference data for urban development and planning in the Xiong'an New Area.

Neuroinflammation is a major feature of type 2 diabetic mellitus (T2DM), adversely affecting hippocampal neurogenesis. However, the precise mechanism is not fully understood, and therapeutic approaches are currently lacking. Therefore, we determined the effects of exercise on neuroinflammation and hippocampal neurogenesis in T2DM mice, with a specific focus on understanding the role of the irisin and related cascade pathways in modulating the beneficial effects of exercise in these processes. Ten-week exercise significantly decreased T2DM-induced inflammation levels and markedly promoted hippocampal neurogenesis and memory function. However, these positive effects were reversed by 10 weeks of treatment with cyclo RGDyk, an inhibitor of irisin receptor signaling. Additionally, exercise helped reduce the M1 phenotype polarization of hippocampal microglia in diabetic mice; this effect could be reversed with cyclo RGDyk treatment. Moreover, exercise markedly increased the levels of fibronectin type III domain-containing protein 5 (FNDC5)/irisin protein while decreasing the expression of Toll-like receptor 4 (TLR4), myeloid differential protein-88 (MyD88), and nuclear factor kappa-B (NF-κB) in the hippocampus of T2DM mice. However, blocking irisin receptor signaling counteracted the down-regulation of TLR4/MyD88/NF-κB in diabetic mice undergoing exercise intervention. Conclusively, exercise appears to be effective in reducing neuroinflammation and enhancing hippocampal neurogenesis and memory in diabetes mice. The positive effects are involved in the participation of the irisin/TLR4/MyD88/NF-κB signaling pathway, highlighting the potential of exercise in the management of diabetic-induced cognitive decline.

(1) Background: While there is extensive documentation on the medical experience of breast cancer, a thorough understanding of the various stages of endocrine therapy remains insufficient. The aim of this study was to map the experiences and coping styles of breast cancer patients during endocrine therapy. (2) Methods: Qualitative research was conducted to gather insights into the experiences of breast cancer patients undergoing endocrine therapy. The themes were organized through content analysis and induction. Subsequently, patients were invited for face-to-face interviews at a top-three hospital in Guangzhou to supplement and validate the findings from the literature review. The patient journey was then mapped based on both the literature review and the semi-structured interviews. (3) Results: A total of 24 studies were included that described patients' experiences and behaviors during the early, middle, and late stages of treatment, leading to the formation of a preliminary framework. Interviews were conducted with 20 patients, which confirmed and enriched the findings from the literature review. Based on these results, a stage trajectory for endocrine therapy in breast cancer was established. (4) Conclusions: The patient journey map developed in this study clearly and intuitively illustrates the thought and emotion matrix, as well as the behavior matrix, of breast cancer patients undergoing endocrine therapy. This provides a theoretical foundation for enhancing clinical services tailored to the needs of these patients.

Inhalation allergies caused by cats and dogs can lead to a range of discomforting symptoms, such as rhinitis and asthma, in humans. With the increasing popularity of and care provided to these companion animals, the allergens they produce pose a growing threat to susceptible patients' health. Allergens from cats and dogs have emerged as significant risk factors for triggering asthma and allergic rhinitis worldwide; however, there remains a lack of systematic measures aimed at assisting individuals in recognizing and preventing allergies caused by these animals. This review provides comprehensive insights into the classification of cat and dog allergens, along with their pathogenic mechanisms. This study also discusses implementation strategies for prevention and control measures, including physical methods, gene-editing technology, and immunological approaches, as well as potential strategies for enhancing allergen immunotherapy combined with immunoinformatics. Finally, it presents future prospects for the prevention and treatment of human allergies caused by cats and dogs. This review will improve knowledge regarding allergies to cats and dogs while providing insights into potential targets for the development of next-generation treatments.

The sugC gene of Streptococcus suis ( S. suis ) is a coding gene for the ATP-binding transporter-associated protein with strong pathogenicity. In order to reveal the effect of the sugC gene on the virulence of S. suis serotype 2, a wild-type strain of TJS75, isolated from fattening pigs' brain tissue samples, was used as a parent strain, and a knockout sugC gene (Δ sugC ) and complementary strain (CΔ sugC ) were successfully constructed via homologous recombination technology. The biological characteristics of TJS75, Δ sugC and CΔ sugC were compared and analyzed through growth curves, biochemical characteristics, hemolysis characteristics, cell infection tests and pathogenicity tests on BALB/c mice. The results of the growth characteristic experiments in vitro showed that the plateau stage growth period of Δ sugC was delayed compared to the TJS75 strain, but there was no difference in the total number of bacteria. The biochemical characteristics and hemolysis ability of Δ sugC in sheep blood had no difference compared with TJS75, but its adhesion and invasion abilities in PK-15 cells were decreased. Knockout of the sugC gene had no impact on the expression levels of adhesion-related genes in TJS75 in real-time PCR analysis. In addition, the LD 50 of Δ sugC in BALB/c mice was 1.47 × 10 8 CFU, seven times higher than that of TJS75 (LD 50 = 2.15 × 10 7 CFU). These results illustrate that the deletion of sugC reduced the virulence of TJS75 to BALB/c mice, but its role in the adhesion and invasion of PK-15 cells in this strain needs to be further explored. In summary, this study provides evidence that the sugC gene is a virulence-related gene in the S. suis serotype 2 strain and plays a crucial role in the adhesion and invasion of S. suis . This study lays a foundation for the further exploration of the potential virulence factors and pathogenesis of S. suis .

Background: In recent years, the advancement of computational chemistry has offered new insights into the rational design of molecularly imprinted polymers (MIPs). From this aspect, our study tried to give quantitative parameters for evaluating imprinting efficiency and exploring the formation mechanism of MIPs by combining simulation and experiments., Methods: The pre-polymerization system of sulfadimethoxine (SDM) was investigated using a combination of quantum chemical (QC) calculations and molecular dynamics (MD) simulations. MIPs were prepared on the surface of silica gel by a surface-initiated supplemental activator and reducing agent atom transfer radical polymerization (SI-SARA ATRP)., Results: The results of the QC calculations showed that carboxylic monomers exhibited higher bonding energies with template molecules than carboxylic ester monomers. MD simulations confirmed the hydrogen bonding sites predicted by QC calculations. Furthermore, it was observed that only two molecules of monomers could bind up to one molecule of SDM, even when the functional monomer ratio was up to 10. Two quantitative parameters, namely, the effective binding number (EBN) and the maximum hydrogen bond number (HBN Max ), were defined. Higher values of EBN and HBN Max indicated a higher effective binding efficiency. Hydrogen bond occupancies and RDF analysis were performed to analyze the hydrogen bond formation between the template and the monomer from different perspectives. Furthermore, under the influence of the EBN and collision probability of the template and the monomers, the experimental results show that the optimal molar ratio of template to monomer is 1:3., Conclusions: The method of monomer screening presented in this study can be extended to future investigations of pre-polymerization systems involving different templates and monomers.

GRK2 and arrestin3, key players in the functional regulation of G protein-coupled receptors (GPCRs), are ubiquitinated by Mdm2, a nuclear protein. The agonist-induced increase in arrestin3 ubiquitination occurs in the nucleus, underscoring the crucial role of its nuclear translocation in this process. The ubiquitination of arrestin3 occurs in the nucleus, highlighting the pivotal role of its nuclear translocation in this process. In contrast, GRK2 cannot translocate into the nucleus; thus, facilitation of the cytosolic translocation of nuclear Mdm2 is required to ubiquitinate GRK2 in the cytosol. Among the explored cellular components and processes, arrestin, Gβγ, clathrin, and receptor phosphorylation were found to be required for the nuclear import of arrestin3, the ubiquitination of arrestin3 in the nucleus, nuclear export of Mdm2, and the ubiquitination of GRK2 in the cytosol. In conclusion, our findings demonstrate that agonist-induced ubiquitination of arrestin3 in the nucleus is interconnected with cytosolic GRK2 ubiquitination.

The emergence of natural products has provided extremely valuable references for the treatment of various diseases. Cucurbitacin B, a tetracyclic triterpenoid compound isolated from cucurbitaceae and other plants, is the most abundant member of the cucurbitin family and exhibits a wide range of biological activities, including anti-inflammatory, anti-cancer, and even agricultural applications. Due to its high toxicity and narrow therapeutic window, structural modification and dosage form development are necessary to address these issues with cucurbitacin B. This paper reviews recent research progress in the pharmacological action, structural modification, and application of cucurbitacin B. This review aims to enhance understanding of advancements in this field and provide constructive suggestions for further research on cucurbitacin B.

Teicoplanin has been banned in the veterinary field due to the drug resistance of antibiotics. However, teicoplanin residue from the antibiotic abuse of humans and animals poses a threat to people's health. Therefore, it is necessary to develop an efficient way for the highly accurate and reliable detection of teicoplanin from humans, food, and water. In this study, novel imprinted quantum dots of teicoplanin were prepared based on boronate affinity-based precisely controlled surface imprinting. The imprinting factor (IF) for teicoplanin was evaluated and reached a high value of 6.51. The results showed excellent sensitivity and selectivity towards teicoplanin. The relative fluorescence intensity was inversely proportional to the concentration of teicoplanin, in the range of 1.0-17 μM. And its limit of detection (LOD) was obtained as 0.714 μM. The fluorescence quenching process was mainly controlled by a static quenching mechanism via the non-radiative electron-transfer process between QDs and the five-membered cyclic boronate esters. The recoveries for the spiked urine, milk, and water samples ranged from 95.33 to 104.17%, 91.83 to 97.33, and 94.22 to 106.67%, respectively.

Lithium metal is regarded as ideal anode material due to its high theoretical specific capacity and low electrode potential. However, the uncontrollable growth of lithium dendrites seriously hinders the practical application of lithium-metal batteries (LMBs). Among various strategies, carbon nanofiber materials have shown great potential in stabilizing the lithium-metal anode (LMA) due to their unique functional and structural characteristics. Here, the latest research progress on carbon nanofibers (CNFs) for LMA is systematically reviewed. Firstly, several common preparation techniques for CNFs are summarized. Then, the development prospects, strategies and the latest research progress on CNFs for dendrite-free LMA are emphatically introduced from the perspectives of neat CNFs and CNF-based composites. Finally, the current challenges and prospects of CNFs for stabilizing LMA are summarized and discussed. These discussions and proposed strategies provide new ideas for the development of high-performance LMBs.

On-site bioaerosol monitoring is essential for estimating microbial biomass and mitigating the risk of infection induced by aerosol transmission. This study introduces a novel electrostatic bioaerosol sampler, which is fabricated by the use of 3D printing, for rapid bioaerosol collection. Aerosol particles were charged and enriched in the sampler. Relationships between particle sizes and collection efficiencies under varying charging voltages were established using a charging model. The design of the sampler was optimized using commercial software, incorporating electrostatic field analysis, computational fluid dynamics (CFD), and particle trajectory simulations. To validate the sampler's collection efficiency, polystyrene (PS) spheres in an aerosol dispenser were atomized into an aerosol. The sampler collection efficiency exceeded 90% for particles larger than 1.2 μm under an applied voltage of 4.7 kV and an airflow rate of 2 L/min. The enrichment capacity was greater than 153,000 for particles larger than 1.2 μm under an applied voltage of 4.7 kV and an airflow rate of 8 L/min. With the merits of low cost, miniaturization, and high collection efficiency, the sampler can be used to collect samples on-site and in remote areas to verify the pathogens and reduce the risk of infection through aerosol transmission.

Sarcopenia is an age-related syndrome characterized by the loss of skeletal muscle mass and function. Community screening, commonly used in early diagnosis, usually lacks features such as real-time monitoring, low cost, and convenience. This study introduces a promising approach to sarcopenia screening by dynamic plantar pressure monitoring. We propose a wearable flexible-printed piezoelectric sensing array incorporating barium titanate thin films. Utilizing a flexible printer, we fabricate the array with enhanced compressive strength and measurement range. Signal conversion circuits convert charge signals of the sensors into voltage signals, which are transmitted to a mobile phone via Bluetooth after processing. Through cyclic loading, we obtain the average voltage sensitivity (4.844 mV/kPa) of the sensing array. During a 6 m walk, the dynamic plantar pressure features of 51 recruited participants are extracted, including peak pressures for both sarcopenic and control participants before and after weight calibration. Statistical analysis discerns feature significance between groups, and five machine learning models are employed to screen for sarcopenia with the collected features. The results show that the features of dynamic plantar pressure have great potential in early screening of sarcopenia, and the Support Vector Machine model after feature selection achieves a high accuracy of 93.65%. By combining wearable sensors with machine learning techniques, this study aims to provide more convenient and effective sarcopenia screening methods for the elderly.

The emergence of drug-resistant mycobacteria has rendered many clinical drugs and regimens ineffective, imposing significant economic and healthcare burden on individuals and society. Repurposing drugs intended for treating other diseases is a time-saving, cost-effective, and efficient approach for identifying excellent antimycobacterial candidates or lead compounds. This study is the first to demonstrate that rupatadine (RTD), a drug used to treat allergic rhinitis, possesses excellent activity against mycobacteria without detectable resistance, particularly Mycobacterium tuberculosis and Mycobacterium marinum , with a minimal inhibitory concentration as low as 3.13 µg/mL. Furthermore, RTD exhibited moderate activity against nonreplicating M. tuberculosis with minimal inhibitory concentrations lower than drugs targeting the cell wall, suggesting that RTD has great potential to be modified and used for the treatment of nonreplicating M. tuberculosis . Additionally, RTD exhibits partial synergistic effects when combined with clofazimine, pretomanid, and TB47 against M. tuberculosis , providing the theoretical foundation for the development of treatment regimens. Transcriptomic profiling leads us to speculate that eight essential genes may be the targets of RTD or may be closely associated with mycobacterial resistance to RTD. In summary, RTD may be a promising hit for further antimycobacterial drug or regimen optimization, especially in the case of nonreplicating mycobacteria.

Wireless sensing is a crucial technology for building smart cities, playing a vital role in applications such as human monitoring, route planning, and traffic management. Analyzing the data provided by wireless sensing enables the formulation of more scientific decisions. The wireless sensing of dynamic events is a significant branch of wireless sensing. Sensing the specific times and durations of dynamic events is a challenging problem due to the dynamic event information is concealed within static environments. To effectively sense the relevant information of event occurrence, we propose a wireless sensing method for dynamic events based on RSSI, named RSSI-WSDE. RSSI-WSDE utilizes variable-length sliding windows and statistical methods to process original RSSI time series, amplifying the differences between dynamic events and static environments. Subsequently, z-score normalization is employed to enhance the comparability of the sensing effects for different dynamic events. Furthermore, by setting the adaptive threshold, the occurrence of dynamic event is sensed and the relevant information is marked on the original RSSI time series. In this study, the sensing performance of RSSI-WSDE was tested in indoor corridors and outdoor urban road environments. The wireless sensing of dynamic events, including walking, running, cycling, and driving, was conducted. The experimental results demonstrate that RSSI-WSDE can accurately sense the occurrence of dynamic events, marking the specific time and duration with millisecond-level precision. Moreover, RSSI-WSDE exhibits robust performance in wireless sensing of dynamic events in both indoor and outdoor environments.

Atypical porcine pestivirus (APPV) can cause congenital tremor type A-II in neonatal piglets, posing a significant threat to swine herd health globally. Our previous study demonstrated that the Mut domains, comprising 112 amino acids at the N-terminus, are the primary functional regions of the E2 protein of APPV. This study identified 14 host cellular proteins that exhibit potential interactions with the Mut domains of the E2 protein using yeast two-hybrid screening. Using bioinformatics analysis, we discovered that the Mut domains of the E2 protein might exert regulatory effects on apoptosis by modulating energy metabolism within the mitochondria. We also conducted co-immunoprecipitation, glutathione S-transferase pull-down, and immunofluorescence assays to confirm the interaction between the Mut domains of the E2 protein and cathepsin H and signal sequence receptor subunit 4 (SSR4). Ultimately, SSR4 enhanced APPV replication in vitro. In summary, our study successfully elucidated the interactions between the Mut domains of the E2 protein and host cell protein, predicted the potential pathways implicated in these interactions, and demonstrated SSR4 involvement in APPV infection. These significant findings contribute valuable knowledge toward a deeper understanding of APPV pathogenesis and the role of the Mut domains of the E2 protein in this intricate process.

Laser bending forming, as a flexible and die-less forming approach, facilitates the three-dimensional shaping of sheets through the generation of thermal stress via laser-material interaction. In this study, the bending forming characteristics of CoCrFeMnNi high-entropy alloy sheets induced by nanosecond pulse laser irradiation were systematically investigated. The effects of parameters including laser power, scanning speed, number of scans, scanning interval, and sheet size on the bending angle, cross-sectional morphology, and hardness were studied in detail under both the laser single-line and multi-line scanning modes. The experimental results confirmed the effectiveness of nanosecond pulse laser irradiation for achieving accurate formation of CoCrFeMnNi sheets, with the successful fabrication of J, L, and U-shaped metal components. Apart from the forming ability, the cross-sectional hardness was significantly increased due to the grain refinement effect of nanosecond pulse laser irradiation. Furthermore, employing the laser single-line scanning mode enabled the effective rectification of overbending parts, showcasing complete recovery for small-angle overbending, and a remarkable 91% recovery for larger-angle overbending. This study provides an important basis for the bendability of CoCrFeMnNi sheets by laser forming and elucidates the evolution of the microstructure and mechanical properties in the bending region.

Aiming at the complex characteristics of negative pressure waves in low-pressure pipelines inside of buildings, we proposed an estimation method of pressure fluctuation trends based on the robust Kalman filter and the improved VMD, which can be used for leakage detection. The reconstructed baseline signal can accurately describe the fluctuation trend of the negative pressure wave after the pressure drop, and quantitatively express the characteristic difference between the leakage condition and the gas usage condition. The robust Kalman filter was used to estimate the pressure fluctuations. The parameters of VMD were adaptively calculated based on the WAA and discrete scale space. The trend components contained in the IMFs were separated by a reconstruction based on the Fourier series. Based on the simulation signal, the method can accurately restore the trend component contained in the complex pressure signal. Based on the actual signals, the accuracy of small leakage detection is 96.7% and the accuracy of large leakage detection is 73.3%., Competing Interests: Author Xinghao Tian was employed by the company China Construction First Division Group Construction & Development Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Ammonia (NH 3 ) potentially harms human health, the ecosystem, industrial and agricultural production, and other fields. Therefore, the detection of NH 3 has broad prospects and important significance. Ti 3 C 2 T x is a common MXene material that is great for detecting NH 3 at room temperature because it has a two-dimensional layered structure, a large specific surface area, is easy to functionalize on the surface, is sensitive to gases at room temperature, and is very selective for NH 3 . This review provides a detailed description of the preparation process as well as recent advances in the development of gas-sensing materials based on Ti 3 C 2 T x MXene for room-temperature NH 3 detection. It also analyzes the advantages and disadvantages of various preparation and synthesis methods for Ti 3 C 2 T x MXene's performance. Since the gas-sensitive performance of pure Ti 3 C 2 T x MXene regarding NH 3 can be further improved, this review discusses additional composite materials, including metal oxides, conductive polymers, and two-dimensional materials that can be used to improve the sensitivity of pure Ti 3 C 2 T x MXene to NH 3 . Furthermore, the present state of research on the NH 3 sensitivity mechanism of Ti 3 C 2 T x MXene-based sensors is summarized in this study. Finally, this paper analyzes the challenges and future prospects of Ti 3 C 2 T x MXene-based gas-sensitive materials for room-temperature NH 3 detection.

Ultra-high temperature ceramics (UHTCs) have been widely applied in many fields. In order to enhance the comprehensive properties of TaB 2 -based UHTCs, the first collaborative use of fine TaC particles and dispersed multi-walled carbon nanotubes (MWCNTs) was employed via spark plasma sintering (SPS) at 1700 °C. The derived UHTCs exhibited an average grain size of 1.3 μm, a relative density of 98.6%, an elastic modulus of 386.3 GPa, and a nano hardness of 21.7 GPa, leading to a greatly improved oxidation resistance with a lower linear ablation rate at -3.3 × 10 -2 μm/s, and a markedly reinforced ablation resistance with mass ablation rate of -1.3 × 10 -3 mg/(s·cm 2 ). The enhanced ablation resistance was attributable to the physical pinning effect, sealing effect and self-healing effect. Thus, this study provides a potential strategy for preparation of UHTCs with bettered ablation resistance and physical properties.

Objective: To elucidate the chemical profile of Xanthocerais lignum's extracts of different polarities and their impact on rheumatoid arthritis (RA), we identified anti-RA markers and predicted their action mechanisms., Methods: A collagen-induced arthritis rat model was established, and UPLC-Q-Exactive Orbitrap MS technology was employed to analyze and identify the chemical constituents within the alcohol extract of Xanthocerais lignum and its various extraction fractions, as well as their translocation into the bloodstream. Serum spectrum-effect correlation analysis was utilized to elucidate the pharmacodynamic material basis of Xanthocerais lignum against RA and to screen for Q-Markers. Finally, the potential anti-RA mechanisms of the Q-Markers were predicted through compound-target interaction data and validated using molecular docking techniques., Results: We identified 71 compounds, with flavan-3-ols and flavanones as key components. Of these, 36 were detected in the bloodstream, including 17 original and 19 metabolized forms. Proanthocyanidin A2, dihydroquercetin, catechin, and epicatechin (plus glucuronides) showed potential anti-RA activity. These compounds, acting as Q-Markers, may modulate ERK, NF-κB, HIF-1α, and VEGF in the HIF-1 pathway., Conclusions: This research clarifies Xanthocerais lignum's pharmacodynamic material basis against RA, identifies 4 Q-Markers, and offers insights into their mechanisms, aiding quality assessment and lead compound development for RA treatment.

In this paper, we have demonstrated a narrow linewidth high power fiber laser emitting at a short wavelength of ~1050 nm. The fiber laser is based on a structure of master oscillator power amplification (MOPA) with an optimized fiber Bragg-grating-based laser cavity as the seed. Both stimulated Brillouin scattering (SBS) and stimulated Raman scattering (SRS) effects have been effectively suppressed by using a long passive fiber between the seed and the amplifier. Based on the fiber amplifier, we have ultimately boosted the narrow linewidth laser from ~40 W to 3.2 kW with a slope efficiency of 85.1% and a 3-dB linewidth of ~0.1 nm. The SRS suppression ratio of the laser is ~29.7 dB at maximum power. Due to our fiber mode control strategies, the beam quality always stays near-diffraction-limited while amplifying, and the measured M 2 factor is ~1.4 at the maximum power. Further increase in output power is limited by the SBS effect.

The effects of holding time and Si on the content, shape size and structure of Ti 2 Al 20 La phase in Al-Ti-La intermediate alloy were investigated by an X-ray diffractometer, scanning electron microscope and transmission electron microscope. The results show that the volume fraction and aspect ratio of Ti 2 Al 20 La phase in Al-Ti-La intermediate alloy decrease significantly, from 21% and 2.3 without Si addition to 4% and 2.0 with the addition of 2.3 wt.% Si at a holding time of 15 min at 750 °C, respectively. The Si element will attach to the Ti 2 Al 20 La phase and form La-Si binary phase at the grain boundary of α-Al. With the increase of holding time from 15 min to 60 min, the content of Ti 2 Al 20 La phase in the alloy gradually decreases and the size decreases significantly. Meanwhile, Al 11 La 3 will dissolve and disappear, while the content of La-Si binary phase increases, and part of Ti 2 Al 20 La phase transforms into Ti 2 (Al 20- x ,Si x )La phase.

UV-B is an important environmental factor that differentially affects plant growth and secondary metabolites. The effects of supplemental ultraviolet-B (sUV-B) exposure (T1, 1.40 kJ·m -2 ·day -1 ; T2, 2.81 kJ·m -2 ·day -1 ; and T3, 5.62 kJ·m -2 ·day -1 ) on the growth biomass, physiological characteristics, and secondary metabolites were studied. Our results indicated that leaf thickness was significantly ( p < 0.05) reduced under T3 relative to the control (natural light exposure, CK); The contents of 6-BA and IAA were significantly reduced ( p < 0.05); and the contents of ABA, 10-deacetylbaccatin III, and baccatin III were significantly ( p < 0.05) increased under T1 and T2. The paclitaxel content was the highest (0.036 ± 0.0018 mg·g -1 ) under T3. The cephalomannine content was significantly increased under T1. Hmgr gene expression was upregulated under T1 and T3. The gene expressions of Bapt and Dbtnbt were significantly ( p < 0.05) upregulated under sUV-B exposure, and the gene expressions of CoA , Ts , and Dbat were significantly ( p < 0.05) downregulated. A correlation analysis showed that the 6-BA content had a significantly ( p < 0.05) positive correlation with Dbat gene expression. The IAA content had a significantly ( p < 0.05) positive correlation with the gene expression of Hmgr , CoA , Ts , and Dbtnbt . The ABA content had a significantly ( p < 0.05) positive correlation with Bapt gene expression. Dbat gene expression had a significantly ( p < 0.05) positive correlation with the 10-deacetylbaccatin content. Hmgr gene expression was positively correlated with the contents of baccatin III and cephalomannine. Bapt gene expression had a significantly ( p < 0.01) positive correlation with the paclitaxel content. A factor analysis showed that the accumulation of paclitaxel content was promoted under T2, which was helpful in clarifying the accumulation of taxane compounds after sUV-B exposure.

Trichostatin A (TSA), a histone deacetylase (HDAC) inhibitor, promotes the cytotoxicity of the genotoxic anticancer drug cisplatin, yet the underlying mechanism remains poorly understood. Herein, we revealed that TSA at a low concentration (1 μM) promoted the cisplatin-induced activation of caspase-3/6, which, in turn, increased the level of cleaved PARP1 and degraded lamin A&C, leading to more cisplatin-induced apoptosis and G2/M phase arrest of A549 cancer cells. Both ICP-MS and ToF-SIMS measurements demonstrated a significant increase in DNA-bound platinum in A549 cells in the presence of TSA, which was attributable to TSA-induced increase in the accessibility of genomic DNA to cisplatin attacking. The global quantitative proteomics results further showed that in the presence of TSA, cisplatin activated INF signaling to upregulate STAT1 and SAMHD1 to increase cisplatin sensitivity and downregulated ICAM1 and CD44 to reduce cell migration, synergistically promoting cisplatin cytotoxicity. Furthermore, in the presence of TSA, cisplatin downregulated TFAM and SLC3A2 to enhance cisplatin-induced ferroptosis, also contributing to the promotion of cisplatin cytotoxicity. Importantly, our posttranslational modification data indicated that acetylation at H4K8 played a dominant role in promoting cisplatin cytotoxicity. These findings provide novel insights into better understanding the principle of combining chemotherapy of genotoxic drugs and HDAC inhibitors for the treatment of cancers.

Polymer microspheres with temperature and salt resistance were synthesized using the anti-suspension polymerization method, incorporating the functional monomers AMPS, AM, and AA. To enhance their self-gelling properties, the microspheres were designed with a core-shell structure. The shell is composed of a polymeric surfactant, fatty alcohol polyoxyethylene ether methacrylate (AEOMA), which serves as a thermosensitive crosslinking agent, enabling self-crosslinking upon shell decomposition, addressing compatibility with reservoir pore throat dimensions. Comprehensive characterizations including infrared spectroscopy, scanning electron microscopy, optical microscopy, and laser particle size analysis were conducted. The microspheres exhibited successful synthesis, a nanoscale size, and regular spherical morphology. They demonstrated excellent temperature and salt resistance, making them suitable for high-temperature, high-salinity reservoir profile control. With a stable three-dimensional network structure, the microspheres displayed good expansion behavior due to hydrophilic groups along the polymer chains, resulting in favorable water affinity. Even after aging, the microspheres maintained their gelling state with a distinct and stable microscopic network skeleton. They exhibited superior plugging performance in low-permeability reservoirs, while effectively improving water absorption profiles in reservoirs with permeability contrasts of 10 to 80, thereby enhancing oil recovery.

In this study, juvenile crayfish hatched from the same population were cultured in different growing environments: pond ( D 1 ), paddy field ( D 2 ), and aquaculture barrel ( D 3 ), and fed for 60 days. Crayfishes were selected randomly, females and males, 50 tails each from six groups (D1-♀, D1-♂, D2-♀, D2-♂, D3-♀, D3-♂) to measure the following morphological traits: full length ( X 1 ), body length ( X 2 ), chelicerae length ( X 3 ), chelicerae weight ( X 4 ), cephalothorax length ( X 5 ), cephalothorax width ( X 6 ), cephalothorax height ( X 7 ), eye spacing ( X 8 ), caudal peduncle length ( X 9 ), and caudal peduncle weight ( X 10 ). We found that the coefficient of variation (CV) of X 4 was the largest in each culture mode, and males (28.58%~38.67%) were larger than females (37.76%~66.74%). The CV of X 4 of crayfish cultured in D 1 and D 2 was larger than that of D 3 . All traits except X 8 were positively correlated with body weight ( p < 0.05). After pathway analysis, we found that X 4 , X 5 , X 7 , and X 10 were significantly correlated with the body weight of D1-♀; the equation was Y D1-♀ = -29.803 + 1.249 X 4 + 0.505 X 5 + 0.701 X 7 + 1.483 X 10 ( R 2 = 0.947). However, X 2 , X 4 , and X 6 were significantly correlated with the body weight of D1-♂; the equation was Y D1-♂ = -40.881 + 0.39 X 2 + 0.845 X 4 + 1.142 X 6 ( R 2 = 0.927). In D2-♀, X 1 , X 4 , X 5 , and X 10 were significantly correlated with body weight; the equation was Y D2-♀ = -12.248 + 0.088 X 1 + 1.098 X 4 + 0.275 X 5 + 0.904 X 10 ( R 2 = 0.977). X 4 and X 5 played a major role in the body weight of D2-♂ with the equation: Y D2-♂ = -24.871 + 1.177 X 4 + 0.902 X 5 ( R 2 = 0.973). X 3 and X 10 mainly contributed to the body weight of D3-♀ with the equation: Y D3-♀ = -22.476 + 0.432 X 3 + 3.153 X 10 ( R 2 = 0.976). X 1 and X 4 mainly contributed to the body weight of D3-♂ with the equation: Y D3-♂ = -34.434 + 0.363 X 1 + 0.669 X 4 ( R 2 = 0.918). Comparing the pathway analysis with the gray relation analysis, we could conclude that the traits most correlated with body weight in D1-♀ were X 10 and X 7 ; in D1-♂, X 6 ; in D2-♀, X 10 , X 1 , and X 5 ; in D2-♂, X 5 ; in D3-♀, X 10 ; and in D3-♂, X 4 and X 1 .

Ellagic acid (EA) is a natural polyphenol and possesses excellent in vivo bioactivity and antioxidant behaviors, which play an important role in the treatment of oxidative stress-related diseases, such as cancer. Additionally, EA is also known as a skin-whitening ingredient. The content of EA would determine its efficacy. Therefore, the accurate analysis of EA content can provide more information for the scientific consumption of EA-rich foods and cosmetics. Nevertheless, the analysis of EA in these samples is challenging due to the low concentration level and the presence of interfering components with high abundance. Molecularly imprinted polymers are highly efficient pretreatment materials in achieving specific recognition of target molecules. However, the traditional template molecule (EA) could not be absolutely removed. Hence, template leakage continues to occur during the sample preparation process, leading to a lack of accuracy in the quantification of EA in actual samples, particularly for trace analytes. In addition, another drawback of EA as an imprinting template is that EA possesses poor solubility and a high price. Gallic acid (GA), called dummy templates, was employed for the synthesis of MIPs as a solution to these challenges. The approach used in this study was boronate affinity-based oriented surface imprinting. The prepared dummy-imprinted nanoparticles exhibited several significant advantages, such as good specificity, high binding affinity ((4.89 ± 0.46) × 10 -5 M), high binding capacity (6.56 ± 0.35 mg/g), fast kinetics (6 min), and low binding pH (pH 5.0) toward EA. The reproducibility of the dummy-imprinted nanoparticles was satisfactory. The dummy-imprinted nanoparticles could still be reused even after six adsorption-desorption cycles. In addition, the recoveries of the proposed method for EA at three spiked levels of analysis in strawberry and pineapple were 91.0-106.8% and 93.8-104.0%, respectively, which indicated the successful application to real samples.

The emergence of polarization image sensors presents both opportunities and challenges for real-time full-polarization reconstruction in scene imaging. This paper presents an innovative three-stage interpolation method specifically tailored for monochrome polarization image demosaicking, emphasizing both precision and processing speed. The method introduces a novel linear interpolation model based on polarization channel difference priors in the initial two stages. To enhance results through bidirectional interpolation, a continuous adaptive edge detection method based on variance differences is employed for weighted averaging. In the third stage, a total intensity map, derived from the previous two stages, is integrated into a residual interpolation process, thereby further elevating estimation precision. The proposed method undergoes validation using publicly available advanced datasets, showcasing superior performance in both global parameter evaluations and local visual details when compared with existing state-of-the-art techniques.

In this study, TiO 2 /P, K-containing grapefruit peel biochar (TiO 2 /P, K-PC) composites were synthesized in situ biomimetically using grapefruit peel as the bio-template and carbon source and tetrabutyl titanate as the titanium source. This was achieved using the two-step rotary impregnation-calcination method. Adjusting the calcination temperature of the sample in an air atmosphere could regulate the mass ratio of TiO 2 to carbon. The prepared samples were subjected to an analysis of their compositions, structures, morphologies, and properties. It demonstrated that the prepared samples were complexes of anatase TiO 2 and P, K-containing carbon, with the presence of graphitic carbon. They possessed a unique morphological structure with abundant pores and a large surface area. The grapefruit peel powder played a crucial role in the induction and assembly of TiO 2 /P, K-PC composites. The sample PCT-400-550 had the best photocatalytic activity, with the degradation rate of RhB, MO, and MB dye solutions reaching more than 99% within 30 min, with satisfactory cyclic stability. The outstanding photocatalytic activity can be credited to its unique morphology and the efficient collaboration between TiO 2 and P, K-containing biochar.

Numerous post-translational modifications are involved in oocyte maturation and embryo development. Recently, lactylation has emerged as a novel epigenetic modification implicated in the regulation of diverse cellular processes. However, it remains unclear whether lactylation occurs during oocyte maturation and embryo development processes. Herein, the lysine lactylation (Kla) modifications were determined during mouse oocyte maturation and early embryo development by immunofluorescence staining. Exogenous lactate was supplemented to explore the consequences of modulating histone lactylation levels on oocyte maturation and embryo development processes by transcriptomics. Results demonstrated that lactylated proteins are widely present in mice with tissue- and cell-specific distribution. During mouse oocyte maturation, immunofluorescence for H3K9la, H3K14la, H4K8la, and H4K12la was most intense at the germinal vesicle (GV) stage and subsequently weakened or disappeared. Further, supplementing the culture medium with 10 mM sodium lactate elevated both the oocyte maturation rate and the histone Kla levels in GV oocytes, and there were substantial increases in Kla levels in metaphase II (MII) oocytes. It altered the transcription of molecules involved in oxidative phosphorylation. Moreover, histone lactylation levels changed dynamically during mouse early embryogenesis. Sodium lactate at 10 mM enhanced early embryo development and significantly increased lactylation, while impacting glycolytic gene transcription. This study reveals the roles of lactylation during oocyte maturation and embryo development, providing new insights to improving oocyte maturation and embryo quality.

Three pairs of enantiomers ( 1 - 3 )-the new 12 R -aloesol ( 1a ) and two new fatty acids ( 2 and 3 )-and one new natural product ( 4 ) together three known compounds ( 5 - 7 ) were isolated from a coral-reef-derived Streptomyces sp. SCSIO 66814. Their structures were determined through extensive spectroscopic analysis, chiral analysis, and single-crystal X-ray diffraction data. Compounds 2 and 3 were presumed to be intermediates for further generating homononactic acid ( 5 ) and nonactic acid, and the latter two molecules were able to act as precursors to form macrotetrolides with remarkable biological activity. The isolation of related precursors, compounds 2 - 5 , provided more evidence to support the proposal of a plausible biosynthetic pathway for nonactic acid and its homologs. Additionally, (+)- 1 exhibited a weak activity against DPPH radicals.

Soft robots with good deformability and adaptability have important prospects in the bionics and intelligence field. However, current research into soft robots is primarily limited to the study of actuators and ignores the integrated use of functional devices and actuators. To enrich the functions of soft robots and expand their application fields, it is necessary to integrate various functional electronic devices into soft robots to perform diverse functions during dynamic deformation. Therefore, this paper discusses methods and strategies to manufacture optical stimuli-responsive soft actuators and integrate them into functional devices for soft robots. Specifically, laser cutting allows us to fabricate an optically responsive actuator structure, e.g., the curling direction can be controlled by adjusting the direction of the cutting line. Actuators with different bending curvatures, including nonbending, can be obtained by adjusting the cutting depth, cutting width, and the spacing of the cutting line, which makes it easy to obtain a folded structure. Thus, various actuators with complex shape patterns can be obtained. In addition, we demonstrate a fabrication scheme for a worm-like soft robot integrated with functional devices (LEDs are used in this paper). The local nonbending design provides an asymmetric structure that provides driving power and avoids damage to the functional circuit caused by the large deformation during movement. The integration of drive and function provides a new path for the application of soft robots in the intelligence and bionics field.

An ethyl acetate extract of a marine actinomycete strain, Nocardiopsis mentallicus SCSIO 53858, isolated from a deep-sea sediment sample in the South China Sea, exhibited anti-quorum-sensing (QS) activity against Chromobacterium violaceum CV026. Guided by the anti-QS activity, a novel active compound was isolated and purified from the extract and was identified as 2,3-dimethoxycinnamic acid (2,3-DCA) through spectral data analysis. At a concentration of 150 μg/mL, 2,3-DCA exhibited robust inhibitory effects on three QS-regulated traits of C . violaceum CV026: violacein production, swarming motility, and biofilm formation, with inhibition rates of 73.9%, 65.9%, and 37.8%, respectively. The quantitative reverse transcription polymerase chain reaction results indicated that 2,3-DCA can disrupt the QS system in C . violaceum CV026 by effectively suppressing the expression of QS-related genes, including cviR , vioA , vioB , and vioE . Molecular docking analysis revealed that 2,3-DCA hinders the QS system by competitively binding to the same binding pocket on the CviR receptor as the natural signal molecule N-hexanoyl-L-homoserine lactone. Collectively, these findings suggest that 2,3-DCA exhibits promising potential as an inhibitor of QS systems, providing a potential solution to the emerging problem of bacterial resistance.

In this research, an ammonium perchlorate/polydopamine (AP/PDA) core-shell composite was prepared in a non-aqueous solution to reduce the mechanical sensitivity of ammonium perchlorate (AP). The result showed that the AP/PDA core-shell composite could be successfully constructed in ethyl acetate solution with an AP recovery rate that reached 86%. The mechanical sensitivity of the obtained AP/PDA core-shell composite was significantly reduced with a PDA content of only 0.76%. The DSC and TG also indicated that the coating of PDA showed catalytic activity in the thermal decomposition of AP with a lower decomposition temperature and a decreased E a value of AP. Thus, this study proposed a simple strategy for achieving a good balanced between harnessing the energy and ensuring the safety of ammonium perchlorate by significantly reducing its mechanical sensitivity by using a very low polydopamine coating layer content, and this shows great potential for the design and fabrication of insensitive energetic composites for use in propellants., Competing Interests: Authors Yafeng Huang and Xuan Tian were employed by the company Xi&rsquo;an Modern Chemistry Research Institute. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Inflammation is a pivotal factor in the development and advancement of conditions like NAFLD and asthma. Diet can affect several phases of inflammation and significantly influence multiple inflammatory disorders. Siraitia grosvenorii , a traditional Chinese edible and medicinal plant, is considered beneficial to health. Flavonoids can suppress inflammatory cytokines, which play a crucial role in regulating inflammation. In the present experiments, kaempferol 3- O - α -L-rhamnoside-7- O - β -D-xylosyl(1→2)- O - α -L-rhamnoside (SGPF) is a flavonoid glycoside that was first isolated from S . grosvenorii . A series of experimental investigations were carried out to investigate whether the flavonoid component has anti-inflammatory and hepatoprotective effects in this plant. The researchers showed that SGPF has a stronger modulation of protein expression in LPS-induced macrophages (MH-S) and OA-induced HepG2 cells. The drug was dose-dependent on cells, and in the TLR4/NF-κB/MyD88 pathway and Nrf2/HO-1 pathway, SGPF regulated all protein expression. SGPF has a clear anti-inflammatory and hepatoprotective function in inflammatory conditions.

Aliphatic polyester is an important polyester material with good biocompatibility and degradability, which can be synthesized through ring-opening alternating copolymerization (ROAC) of epoxides and anhydrides. Herein, density functional theory (DFT) is used to explore the mechanism of ROAC of epoxides (propylene oxide (PO), styrene oxide (SO), epichlorohydrin (ECH), and cyclohexane oxide (CHO)) and phthalic anhydride (PA) catalyzed by bis(triphenylphosphine) ammonium chloride (PPNCl) and ureas. It was found that the ring-opening polymerization (ROP) of epoxides is the rate-controlling step, and the benzyl alcohol (BnOH) as the initiator has little effect on the polymerization activity, which was consistent with previous experimental results. Calculated comparisons of the ROAC activity of CHO/PA catalyzed by four different ureas indicate that as the Lewis acidity of the urea increased, the energy barriers of the copolymerization increased and the activity decreased. The main reason was that the strong hydrogen-bonding interactions stabilized the key intermediate of the rate-controlling step and inhibited subsequent monomer insertion. Based on this, a series of new ureas with higher catalytic activity were designed by introducing electron-donating substituents. In SO polymerization, increasing the Lewis acidity of urea can improve the SO regioselectivity. In addition, the monomer ECH with CH 2 Cl shows higher activity of ROAC than PO and SO, which could be ascribed to the fact that the strong electron-withdrawing Cl atom stabilizes the transition state in the rate-controlling step and reduces the reaction energy barrier.

Lineament is a unique geological structure. The study of Lunar lineament structure has great significance on understanding its history and evolution of Lunar surface. However, the existing geographic feature extraction methods are not suitable for the extraction of Lunar lineament structure. In this paper, a new lineament extraction method is proposed based on improved-UNet++ and YOLOv5. Firstly, new lineament dataset is created containing lineaments structure based on CCD data from LROC. At same time the residual blocks are replaced with the VGG blocks in the down sample part of the UNet++ with adding the attention block between each layer. Secondly, the improved-UNet++ and YOLO networks are trained to execute the object detection and semantic segmentation of lineament structure respectively. Finally, a polygon-match strategy is proposed to combine the results of object detection and semantic segmentation. The experiment result indicate that this new method has relatively better and more stable performance compared with current mainstream networks and the original UNet++ network in the instance segmentation of lineament structure. Additionally, the polygon-match strategy is able to perform preciser edge detail in the instance segmentation of lineament structure result.

Ozone (O 3 ) is a critical gas in various industrial applications, particularly in semiconductor manufacturing, where it is used for wafer cleaning and oxidation processes. Accurate and reliable detection of ozone concentration is essential for process control, ensuring product quality, and safeguarding workplace safety. By studying the UV absorption characteristics of O 3 and combining the specific operational needs of semiconductor process gas analysis, a pressure-insensitive ozone gas sensor has been developed. In its optical structure, a straight-through design without corners was adopted, achieving a coupling efficiency of 52% in the gas chamber. This device can operate reliably in a temperature range from 0 °C to 50 °C, with only ±0.3% full-scale error across the entire temperature range. The sensor consists of a deep ultraviolet light-emitting diode in a narrow spectrum centered at 254 nm, a photodetector, and a gas chamber, with dimensions of 85 mm × 25 mm × 35 mm. The performance of the sensor has been meticulously evaluated through simulation and experimental analysis. The sensor's gas detection accuracy is 750 ppb, with a rapid response time ( t 90 ) of 7 s, and a limit of detection of 2.26 ppm. It has the potential to be applied in various fields for ozone monitoring, including the semiconductor industry, water treatment facilities, and environmental research.

Alveolar macrophages play a vital role in a variety of lung diseases, including tuberculosis. Thus, alveolar macrophage targeted anti-tubercular drug delivery through nanocarriers could improve its therapeutic response against tuberculosis. The current study aimed at exploring the efficacy of glyceryl monostearate (GMS)-based solid-lipid nanoparticles (SLNs) and their mannose functionalized forms on the alveolar macrophage targeting ability of an anti-tubercular model drug, rifampicin (Rif). Rif-loaded SLNs were accomplished by the solvent diffusion method. These carriers with unimodal particle size distribution (~170 nm) were further surface-modified with mannose via Schiff-base reaction, leading to slight enhancement of particle diameter and a decline of drug loading capacity. The encapsulated Rif, which was molecularly dispersed within the matrices as indicated by their XRD patterns, was eluted in a sustained manner with an initial burst release effect. The uptake efficiency of mannose-modified SLNs was remarkably higher than that of corresponding native forms on murine macrophage Raw 264.7 cells and human lung adenocarcinoma A549 cells. Eventually, the mannose-modified SLNs showed a greater cytotoxicity on Raw 264.7 and A549 cells relative to their unmodified forms. Overall, our study demonstrated that mannose modification of SLNs had an influence on their uptake by alveolar macrophages, which could provide guidance for the future development of alveolar macrophage targeted nanoformulations.

Metal oxide gas sensors usually require a few tens of milliwatts of power consumption to operate at high temperature, which limits their application in mobile and portable devices. Here, we proposed a cantilever structure to build an ultra-low power gas sensor for hydrogen sulfide gas detection. By employing a nano-film size effect to reduce the thermal conductivity of the material, and self-heated corrugation configuration, the power consumption of the gas sensor is significantly reduced. Through numerical analysis and finite element simulation, two different gas sensors were designed and the power consumption and stress distribution were analyzed and optimized. Under the operating temperature of 200 °C, only 0.27 mW power is consumed, the stress value is less than 250 MPa and the displacement is a few hundred of nanometers. The results serve as a guide and reference for ultra-low power MEMS device designs.

Strophanthidin (SPTD), one of the cardiac glycosides, is refined from traditional Chinese medicines such as Semen Lepidii and Antiaris toxicaria , and was initially used for the treatment of heart failure disease in clinic. Recently, SPTD has been shown to be a potential anticancer agent, but the underlying mechanism of action is poorly understood. Herein, we explored the molecular mechanism by which SPTD exerts anticancer effects in A549 human lung adenocarcinoma cells by means of mass spectrometry-based quantitative proteomics in combination with bioinformatics analysis. We revealed that SPTD promoted the expression of tumor necrosis factor (TNF)-related apoptosis-inducing ligand receptor 2 (TRAIL-R2, or DR5) in A549 cells to activate caspase 3/6/8, in particular caspase 3. Consequently, the activated caspases elevated the expression level of apoptotic chromatin condensation inducer in the nucleus (ACIN1) and prelamin-A/C (LMNA), ultimately inducing apoptosis via cooperation with the SPTD-induced overexpressed barrier-to-autointegration factor 1 (Banf1). Moreover, the SPTD-induced DEPs interacted with each other to downregulate the p38 MAPK/ERK signaling, contributing to the SPTD inhibition of the growth of A549 cells. Additionally, the downregulation of collagen COL1A5 by SPTD was another anticancer benefit of SPTD through the modulation of the cell microenvironment.