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ERA5-Land is a valuable reanalysis data resource that provides near-real-time, high-resolution, multivariable data for various applications. Using daily precipitation data from 301 meteorological stations in the Yellow River Basin from 2001 to 2013 as benchmark data, this study aims to evaluate ERA5-Land's capability of monitoring extreme precipitation. The evaluation study is conducted from three perspectives: precipitation amount, extreme precipitation indices, and characteristics of extreme precipitation events. The results show that ERA5-Land can effectively capture the spatial distribution patterns and temporal trends in precipitation and extreme precipitation; however, it also exhibits significant overestimation and underestimation errors. ERA5-Land significantly overestimates total precipitation and indices for heavy precipitation and extreme precipitation (R95pTOT and R99pTOT), with errors reaching up to 89%, but underestimates the Simple Daily Intensity Index (SDII). ERA5-Land tends to overestimate the duration of extreme precipitation events but slightly underestimates the total and average precipitation of these events. These findings provide a scientific reference for optimizing the ERA5-Land algorithm and for users in selecting data. [ABSTRACT FROM AUTHOR]

Integrated Multi-satellitE Retrievals for GPM (Global Precipitation Measurement) (IMERG) is the primary high spatiotemporal resolution precipitation product of the GPM era. To assess the applicability of the latest released IMERG_V07 in mainland China, this study systematically evaluates the error characteristics of IMERG_V07 from the perspective of different seasons, precipitation intensity, topography, and climate regions on an hourly scale. Ground-based meteorological observations are used as the reference, and the performance improvement of IMERG_V07 relative to IMERG_V06 is verified. Error evaluation is conducted in terms of precipitation amount and precipitation frequency, and an improved error component procedure is utilized to trace the error sources. The results indicate that IMERG_V07 exhibits a smaller RMSE in mainland China, especially with significant improvements in the southeastern region. IMERG_V07 shows better consistency with ground station data. IMERG_V07 shows an overall improvement of approximately 4% in capturing regional average precipitation events compared to IMERG_V06, with the northwest region showing particularly notable enhancement. The error components of IMERG_V06 and IMERG_V07 exhibit similar spatial distributions. IMERG_V07 outperforms V06 in terms of lower Missed bias but slightly underperforms in Hit bias and False bias compared to IMERG_V06. IMERG_V07 shows improved ability in capturing precipitation frequency for different intensities, but challenges remain in capturing heavy precipitation events, missing light precipitation, and winter precipitation events. Both IMERG_V06 and IMERG_V07 exhibit notable topography dependency in terms of Total bias and error components. False bias is the primary error source for both versions, except in winter, where high-altitude regions (DEM > 1200 m) primarily contribute to Missed bias. IMERG_V07 has enhanced the accuracy of precipitation retrieval in high-altitude areas, but there are still limitations in capturing precipitation events. Compared to IMERG_V06, IMERG_V07 demonstrates more concentrated error component values in the four climatic regions, with reduced data dispersion and significant improvement in Missed bias. The algorithm improvements in IMERG_V07 have the most significant impact in arid regions. False bias serves as the primary error source for both satellite-based precipitation estimations in the four climatic regions, with a secondary contribution from Hit bias. The evaluation results of this study offer scientific references for enhancing the algorithm of IMERG products and enhancing users' understanding of error characteristics and sources in IMERG. [ABSTRACT FROM AUTHOR]

A metal-free electrochemical oxidative difluoroethylation of 2-arylbenzimidazoles was accomplished, which provided an efficient strategy for the synthesis of MeCF2-containing benzo[4,5]imidazo[2,1-a]-isoquinolin-6(5H)-ones. In addition, the method also enabled the efficient construction of various difluoroethylated indolo[2,1-a]isoquinolin-6(5H)-ones. Notably, this electrochemical synthesis protocol proceeded well under mild conditions without metal catalysts or exogenous additives/oxidants added. [ABSTRACT FROM AUTHOR]

A thorough evaluation of the recently released Global Satellite Mapping of Precipitation (GSMaP) is critical for both end-users and algorithm developers. In this study, six products from three versions of GSMaP version 8, including real time (NOW-R and NOW-C), near real time (NRT-R and NRT-C), and post-real time (MVK-R and MVK-C), are systematically and quantitatively evaluated based on time-by-time observations from 2167 stations in mainland China. Among each version, both products with and without gauge correction are adopted to detect the gauge correction effect. Error quantification is carried out on an hourly timescale. Three common statistical indices (i.e., correlation coefficient (CC), relative bias (RB), and root mean square error (RMSE)) and three event detection capability indices (i.e., probability of detection (POD), false alarm ratio (FAR), and critical success index (CSI)) were adopted to analyze the inversion errors in precipitation amount and precipitation event frequency across the various products. Additionally, in this study, we examine the dependence of GSMaP errors on rainfall intensity and elevation. The following main results can be concluded: (1) MVK-C exhibits the best ability to retrieve rainfall on the hourly timescale, with higher CC values (0.31 in XJ to 0.47 in SC), smaller RMSE values (0.14 mm/h in XJ to 0.99 mm/h in SC), and lower RB values (−4.78% in XJ to 16.03% in NC). (2) Among these three versions, the gauge correction procedure plays a crucial role in reducing errors, especially in the post-real-time version. After being corrected, MVK-C demonstrates an obvious CC value improvement (>0.3 on the hourly timescale) in various sub-regions, increasing the percentage of sites with CC values above 0.5 from 0.03% (MVK-R) to 28.47% (MVK-C). (3) GSMaP products generally exhibit error dependencies on precipitation intensity and elevation, particularly in areas with drastic elevation changes (such as 1200–1500 m and 3000–3300 m), where the accuracy of satellite precipitation estimates is significantly affected. (4) CC values decreased with an increasing rainfall intensity; RB and RMSE values increased with an increasing rainfall intensity. The results of this study may be helpful for algorithm developers and end-users and provide a scientific reference for different hydrological applications and disaster risk reduction. [ABSTRACT FROM AUTHOR]

Radiation-induced skin injury (RISI) is a frequent and severe complication with a complex pathogenesis that often occurs during radiation therapy, nuclear incidents, and nuclear war, for which there is no effective treatment. Hyaluronan (HA) plays an overwhelming role in the skin, and it has been shown that UVB irradiation induces increased HA expression. Nevertheless, to the best of our knowledge, there has been no study regarding the biological correlation between RISI and HA degradation and its underlying mechanisms. Therefore, in our study, we investigated low-molecular-weight HA content using an enzyme-linked immunosorbent assay and changes in the expression of HA-related metabolic enzymes using real-time quantitative polymerase chain reaction and a Western blotting assay. The oxidative stress level of the RISI model was assessed using sodium dismutase, malondialdehyde, and reactive oxygen species assays. We demonstrated that low-molecular-weight HA content was significantly upregulated in skin tissues during the late phase of irradiation exposure in the RISI model and that HA-related metabolic enzymes, oxidative stress levels, the MEK5/ERK5 pathway, and inflammatory factors were consistent with changes in low-molecular-weight HA content. These findings prove that HA degradation is biologically relevant to RISI development and that the HA degradation mechanisms are related to HA-related metabolic enzymes, oxidative stress, and inflammatory factors. The MEK5/ERK5 pathway represents a potential mechanism of HA degradation. In conclusion, we aimed to investigate changes in HA content and preliminarily investigate the HA degradation mechanism in a RISI model under γ-ray irradiation, to consider HA as a new target for RISI and provide ideas for novel drug development. [ABSTRACT FROM AUTHOR]

The direct oxidative alkenylation of indoles is achieved by ferrous salts under mild conditions, which provides one effective strategy for the synthesis of 3-alkylideneindolin-2-one in a single step. This reaction system features simple and readily available materials, mild conditions, and easy accessibility. The control experiments also demonstrate a radical pathway was involved in the reaction. Moreover, the method performs well on the gram-scale experiment, which indicates that this method enjoys a broad prospect in synthetic chemistry. [ABSTRACT FROM AUTHOR]

The CD69 molecule, as an early activation marker of lymphocytes, is often used to assess the activation of cellular immunity. However, for pigs, an anti-pig CD69 antibody is not yet available for this purpose after infection or vaccination. In this study, a monoclonal antibody (mAb) against pig CD69 was produced by peptide immunization and hybridoma technique. One mAb (5F12) showed good reactivity with pig CD69 that was expressed in transfected-HEK-293T cells and on mitogen-activated porcine peripheral blood mononuclear cells (PBMCs) by indirect immunofluorescence assay and flow cytometry. This mAb did not cross-react with activated lymphocytes from mouse, bovine, and chicken. Epitope mapping showed that the epitope recognized by this mAb was located at amino acid residues 147–161 of pig CD69. By conjugating with fluorochrome, this mAb was used to detect the early activation of lymphocytes in PRRSV- and ASFV-infected pigs by flow cytometry. The results showed that PRRSV infection induced the dominant activation of CD4 T cells in mediastinal lymph nodes and CD8 T cells in the spleen at 14 days post-infection, in terms of CD69 expression. In an experiment on ASFV infection, we found that ASFV infection resulted in the early activation of NK cells, B cells, and distinct T cell subsets with variable magnitude in PBMCs, spleen, and submandibular lymph nodes. Our study revealed an early event of lymphocyte and T cell activation after PRRSV and ASFV infections and provides an important immunological tool for the in-depth analysis of cellular immune response in pigs after infection or vaccination. [ABSTRACT FROM AUTHOR]

In this study, the failure processes of modeled concrete specimens under shear force were studied. To investigate the cracks propagation and failure modes, modeled natural aggregate, modeled recycled aggregate, and modeled brick aggregate were used as coarse aggregate in the modeled concrete. Through digital image correlation (DIC) technology, the displacement field and strain field could be obtained by analyzing the change of the speckle position on the surface of the specimens. It was found that the shear strength of the modeled natural concrete (MNC) was the highest, the modeled recycled concrete (MRC) was the second, and the modeled brick aggregate concrete (MBC) was the lowest and only half of that of MNC. The shear crack of the MNC first appeared at the interface transition zone (ITZ) and propagated, resulting in the shear failure. The shear crack of MRC first appeared in the new ITZ and then expanded to the old ITZ. The shear crack of MBC extended through the brick aggregate. The study also found that, along with the compressive stress trace range, the MNC and the MRC have four vertical compressive strain concentration zones, while the MBC has only two strain concentration zones, which should result from the fact that the elastic modulus of the brick aggregate is lower than the surrounding hardened mortar. Therefore, there are no compressive strain concentration zones near the brick aggregate. [ABSTRACT FROM AUTHOR]