1. Bacillus Cyclic Lipopeptides Iturin and Fengycin Control Rice Blast Caused by Pyricularia oryzae in Potting and Acid Sulfate Soils by Direct Antagonism and Induced Systemic Resistance
- Author
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Thibault Meyer, Feyisara Eyiwumi Oni, Monica Höfte, Marc Ongena, Van Bach Lam, Anthony Arguelles Arias, Universiteit Gent = Ghent University [Belgium] (UGENT), Université de Liège, Laboratoire d'Ecologie Microbienne - UMR 5557 (LEM), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Ecole Nationale Vétérinaire de Lyon (ENVL)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Résistance Induite et Bioprotection des Plantes - EA 4707 (RIBP), Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Agriculture and Food Sciences ,0106 biological sciences ,0301 basic medicine ,Microbiology (medical) ,Pyricularia ,acid sulfate soil ,QH301-705.5 ,ved/biology.organism_classification_rank.species ,BIOLOGY ,Bacillus altitudinis ,Fungus ,Biology ,SUBTILIS ,01 natural sciences ,Microbiology ,surfactin ,Article ,03 medical and health sciences ,chemistry.chemical_compound ,PLANT INFECTION ,Virology ,Bacillus velezensis ,Spore germination ,Pyricularia oryzae ,Biology (General) ,Mycelium ,2. Zero hunger ,ved/biology ,fengycin ,rice blast ,food and beverages ,iturin ,biology.organism_classification ,Potting soil ,BIOCONTROL ,YIELD ,030104 developmental biology ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,FUNGUS ,chemistry ,Antagonism ,Surfactin ,010606 plant biology & botany - Abstract
Rice monoculture in acid sulfate soils (ASSs) is affected by a wide range of abiotic and biotic constraints, including rice blast caused by Pyricularia oryzae. To progress towards a more sustainable agriculture, our research aimed to screen the biocontrol potential of indigenous Bacillus spp. against blast disease by triggering induced systemic resistance (ISR) via root application and direct antagonism. Strains belonging to the B. altitudinis and B. velezensis group could protect rice against blast disease by ISR. UPLC–MS and marker gene replacement methods were used to detect cyclic lipopeptide (CLiP) production and construct CLiPs deficient mutants of B. velezensis, respectively. Here we show that the CLiPs fengycin and iturin are both needed to elicit ISR against rice blast in potting soil and ASS conditions. The CLiPs surfactin, iturin and fengycin completely suppressed P. oryzae spore germination resulting in disease severity reduction when co-applied on rice leaves. In vitro microscopic assays revealed that iturin and fengycin inhibited the mycelial growth of the fungus P. oryzae, while surfactin had no effect. The capacity of indigenous Bacillus spp. to reduce rice blast by direct and indirect antagonism in ASS conditions provides an opportunity to explore their usage for rice blast control in the field.
- Published
- 2021
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