Search

The basic helix–loop–helix (bHLH) gene family is a crucial regulator in plants, orchestrating various developmental processes, particularly flower formation, and mediating responses to hormonal signals. The molecular mechanism of bamboo flowering regulation remains unresolved, limiting bamboo breeding efforts. In this study, we identified 309 bHLH genes and divided them into 23 subfamilies. Structural analysis revealed that proteins in specific DlbHLH subfamilies are highly conserved. Collinearity analysis indicates that the amplification of the DlbHLH gene family primarily occurs through segmental duplications. The structural diversity of these duplicated genes may account for their functional variability. Many DlbHLHs are expressed during flower development, indicating the bHLH gene's significant role in this process. In the promoter region of DlbHLHs, different homeopathic elements involved in light response and hormone response co-exist, indicating that DlbHLHs are related to the regulation of the flower development of D. latiflorus. [ABSTRACT FROM AUTHOR]

Zearalenone (ZEN) is a toxic secondary metabolite produced by the Fusarium fungi, which widely contaminates grains, food, and feed, causing health hazards for humans and animals. Therefore, it is essential to find effective ZEN detoxification methods. Enzymatic degradation of ZEN is believed to be an eco-friendly detoxification strategy, specifically thermostable ZEN degradation enzymes are needed in the food and feed industry. In this study, a novel ZEN lactone hydrolase ZHRnZ from Rosellinia necatrix was discovered using bioinformatic and molecular docking technology. The recombinant ZHRnZ showed the best activity at pH 9.0 and 45 °C with more than 90% degradation for ZEN, α-zearalenol (α-ZOL), β-zearalenol (β-ZOL) and α-zearalanol (α-ZAL) after incubation for 15 min. We obtained 10 mutants with improved thermostability by single point mutation technology. Among them, mutants E122Q and E122R showed the best performance, which retained more than 30% of their initial activity at 50 °C for 2 min, and approximately 10% of their initial activity at 60 °C for 1 min. The enzymatic kinetic study showed that the catalytic efficiency of E122R was 1.3 times higher than that of the wild-type (WT). Comprehensive consideration suggests that mutant E122R is a promising hydrolase to detoxify ZEN in food and feed. [ABSTRACT FROM AUTHOR]

A predictive model utilizing near-infrared spectroscopy was developed to estimate the loss on drying, total contents of crocin I and crocin II, and picrocrocin content of saffron. Initially, the LD values were determined using a moisture-ash analyzer, while HPLC was employed for measuring the total contents of crocin I, crocin II, and picrocrocin. The near-infrared spectra of 928 saffron samples were collected and preprocessed using first derivative, standard normal variable transformation, detrended correction, multivariate scattering correction, Savitzky–Golay smoothing, and mean centering methods. Leveraging the partial least squares method, regression models were constructed, with parameters optimized through a selective combination of the above six preprocessing methods. Subsequently, prediction models for loss on drying, total contents of crocin I and crocin II, and picrocrocin content were established, and the prediction accuracy of the models was verified. The correlation coefficients and root mean square error of loss on drying, total contents of crocin I and crocin II, and picrocrocin content demonstrated high accuracy, with R2 values of 0.8627, 0.8851, and 0.8592 and root mean square error values of 0.0260, 0.0682, and 0.0465. This near-infrared prediction model established in the present study offers a precise and efficient means of assessing loss on drying, total contents of crocin I and crocin II, and picrocrocin content in saffron and is useful for the development of a rapid quality evaluation system. [ABSTRACT FROM AUTHOR]

Transcription factors (TFs) are crucial pre-transcriptional regulatory mechanisms that can modulate the expression of downstream genes by binding to their promoter regions. DOF (DNA binding with One Finger) proteins are a unique class of TFs with extensive roles in plant growth and development. Our previous research indicated that iron content varies among bamboo leaves of different colors. However, to our knowledge, genes related to iron metabolism pathways in bamboo species have not yet been studied. Therefore, in the current study, we identified iron metabolism related (IMR) genes in bamboo and determined the TFs that significantly influence them. Among these, DOFs were found to have widespread effects and potentially significant impacts on their expression. We identified specific DOF members in Dendrocalamus latiflorus with binding abilities through homology with Arabidopsis DOF proteins, and established connections between some of these members and IMR genes using RNA-seq data. Additionally, molecular docking confirmed the binding interactions between these DlDOFs and the DOF binding sites in the promoter regions of IMR genes. The co-expression relationship between the two gene sets was further validated using q-PCR experiments. This study paves the way for research into iron metabolism pathways in bamboo and lays the foundation for understanding the role of DOF TFs in D. latiflorus. [ABSTRACT FROM AUTHOR]

Existing studies have examined unhealthy food packaging information, mainly focusing on aspects such as the content, color, and text, whilst paying less attention to the boundaries of information. This paper investigates unhealthy foods through three experiments, revealing that the presence (vs. absence) of packaging information boundaries on unhealthy foods has a negative impact on consumers' purchasing intentions (p = 0.040) (Experiment 1). The feeling of constraint mediates this effect (β = −0.078, CI: [−0.1911, −0.0111]) (Experiment 2). Additionally, consumers with an independent self-construal exhibit reduced purchasing intentions when unhealthy food packaging information boundaries are present (vs. absent) (p < 0.001), whereas those with an interdependent self-construal show increased purchasing intentions under the same conditions (p = 0.024) (Experiment 3). This paper reveals the psychological mechanism and boundary conditions of unhealthy food packaging information boundaries affecting consumers' purchasing intention and provides practical inspiration for government policy-making related to unhealthy food packaging. [ABSTRACT FROM AUTHOR]

Obesity is a multifactorial chronic inflammatory metabolic disorder, with pathogenesis influenced by genetic and non-genetic factors such as environment and diet. Intestinal microbes and their metabolites play significant roles in the occurrence and development of obesity by regulating energy metabolism, inducing chronic inflammation, and impacting intestinal hormone secretion. Epigenetics, which involves the regulation of host gene expression without changing the nucleotide sequence, provides an exact direction for us to understand how the environment, lifestyle factors, and other risk factors contribute to obesity. DNA methylation, as the most common epigenetic modification, is involved in the pathogenesis of various metabolic diseases. The epigenetic modification of the host is induced or regulated by the intestinal microbiota and their metabolites, linking the dynamic interaction between the microbiota and the host genome. In this review, we examined recent advancements in research, focusing on the involvement of intestinal microbiota and DNA methylation in the etiology and progression of obesity, as well as potential interactions between the two factors, providing novel perspectives and avenues for further elucidating the pathogenesis, prevention, and treatment of obesity. [ABSTRACT FROM AUTHOR]

The properties and arrangement of checker bricks in regenerators are crucial for the heat exchange process of hot blast stoves. In this study, a 3D fluid flow heat transfer model is established to analyze the influence of three regenerator layered structures on the combustion and air supply performance of hot blast stoves. The results show that a "silica bricks–high-alumina bricks–clay bricks" three-layer arrangement in regenerators produces a "thermal conduction hindrance effect" at the interface between silica and high-alumina bricks during the 2 h combustion period, which raises the local temperature and improves air supply performance. Compared to the "silica bricks–clay bricks" and "high-alumina bricks–silica bricks–clay bricks" structures, this setup increases the maximum air supply temperature difference to 23 and 64 K, respectively, and extends the effective air supply time by 80 and 320 s, respectively. However, the "thermal conduction hindrance effect" diminishes over longer combustion periods, and by 4 h, the performance across all structures becomes increasingly consistent. Additionally, the study suggests that the temperature level and distribution in the upper part of the regenerator are the key factors determining the air supply performance of hot blast stoves. [ABSTRACT FROM AUTHOR]

In theory, two extreme forms of substances exist: the pure form and the single-molecule mixture form. The latter contains a mixture of molecules with molecularly different structures. Inspired by the "chemical space" concept, in this paper, I report a study of the single-molecule mixture state that combines model construction and mathematical analysis, obtaining some interesting results. These results provide theoretical evidence that the single-molecule mixture state may indeed exist in realistic synthetic or natural polymer systems. [ABSTRACT FROM AUTHOR]

Diabetic complications pose a significant threat to life and have a negative impact on quality of life in individuals with diabetes. Among the various factors contributing to the development of these complications, endothelial dysfunction plays a key role. The main mechanism underlying endothelial dysfunction in diabetes is oxidative stress, which adversely affects the production and availability of nitric oxide (NO). Flavonoids, a group of phenolic compounds found in vegetables, fruits, and fungi, exhibit strong antioxidant and anti-inflammatory properties. Several studies have provided evidence to suggest that flavonoids have a protective effect on diabetic complications. This review focuses on the imbalance between reactive oxygen species and the antioxidant system, as well as the changes in endothelial factors in diabetes. Furthermore, we summarize the protective mechanisms of flavonoids and their derivatives on endothelial dysfunction in diabetes by alleviating oxidative stress and modulating other signaling pathways. Although several studies underline the positive influence of flavonoids and their derivatives on endothelial dysfunction induced by oxidative stress in diabetes, numerous aspects still require clarification, such as optimal consumption levels, bioavailability, and side effects. Consequently, further investigations are necessary to enhance our understanding of the therapeutic potential of flavonoids and their derivatives in the treatment of diabetic complications. [ABSTRACT FROM AUTHOR]

The Internet of Things (IoT) plays an essential role in people's daily lives, such as healthcare, home, traffic, industry, and so on. With the increase in IoT devices, there emerge many security issues of data loss, privacy leakage, and information temper in IoT network applications. Even with the development of quantum computing, most current information systems are weak to quantum attacks with traditional cryptographic algorithms. This paper first establishes a general security model for these IoT network applications, which comprises the blockchain and a post-quantum secure identity-based signature (PQ-IDS) scheme. This model divides these IoT networks into three layers: perceptual, network, and application, which can protect data security and user privacy in the whole data-sharing process. The proposed PQ-IDS scheme is based on lattice cryptography. Bimodal Gaussian distribution and the discrete Gaussian sample algorithm are applied to construct the fundamental difficulty problem of lattice assumption. This assumption can help resist the quantum attack for information exchange among IoT devices. Meanwhile, the signature mechanism with IoT devices' identity can guarantee non-repudiation of information signatures. Then, the security proof shows that the proposed PQ-IDS can obtain the security properties of unforgeability, non-repudiation, and non-transferability. The efficiency comparisons and performance evaluations show that the proposed PQ-IDS has good efficiency and practice in IoT network applications. [ABSTRACT FROM AUTHOR]

As China's urbanization process accelerates, the issue of air pollution becomes increasingly prominent and urgently requires improvement, based on the fact that environmental conditions such as meteorology and topography are difficult to change. Therefore, relevant optimization studies from the perspective of architectural patterns are operable to mitigate pollution. This paper takes the Wenhua Road block in Shenyang, China, as the research object; obtains the concentration data of three kinds of particulate matter through fixed and mobile monitoring; and analyzes the spatial distribution characteristics of Local Climate Zones (LCZ) and particulate matter in the block based on the ArcGIS platform, identifies high-risk areas, and excavates the influence of LCZ on the concentrations of three kinds of particulate matter. The results show that the spatial distribution characteristics of PM1, PM2.5, and PM10 under the same pollution level are relatively similar, while the spatial heterogeneity of the distribution of the same particulate matter under different pollution levels is higher. The time-weighted results show that the PM1 pollution level in the block ranges from 44 to 51 μg/m³, PM2.5 ranges from 75 to 86 μg/m³, and PM10 ranges from 87 to 99 μg/m³. The pollution hot spots throughout the year are located in the central, eastern and western parts of the study area. In terms of the relationship between the LCZ and particulate matter, with the increase in the particulate matter diameter, the correlation between the three kinds of particulate matter and LCZ are all enhanced. The built-up LCZ always has a larger average concentration of particulate matter than that of the natural LCZ, and building height and building density are the main factors causing the difference. In the optimal design of the risk area, the proportion of natural vegetation or water surface should be increased and the building height should be properly controlled and the building density should be reduced in the renewal of the urban building form. This study will largely improve the spatial refinement of the optimization of urban architectural patterns oriented to mitigate particulate matter pollution. [ABSTRACT FROM AUTHOR]

Path planning algorithms are crucial components in the process of smart parking. At present, there are many path planning algorithms designed for smart parking. A well-designed path planning algorithm has a significant impact on the efficiency of smart parking. Firstly, this paper comprehensively describes the principles and steps of four types of path planning algorithms: the Dijkstra algorithm (including its optimized derivatives), the A* algorithm (including its optimized derivatives), the RRT (Rapidly exploring Random Trees) algorithm (including its optimized derivatives), and the BFS (Breadth First Search) algorithm. Secondly, the Dijkstra algorithm, the A* algorithm, the BFS algorithm, and the Dynamic Weighted A* algorithm were utilized to plan the paths required for the process of smart parking. During the analysis, it was found that the Dijkstra algorithm had the drawbacks of planning circuitous paths and taking too much time in the path planning for smart parking. Although the traditional A* algorithm based on the Dijkstra algorithm had greatly reduced the planning time, the effect of path planning was still unsatisfactory. The BFS (Breadth First Search) algorithm had the shortest planning time among the four algorithms, but the paths it plans were unstable and not optimal. The Dynamic Weighted A* algorithm could achieve better path planning results, and with adjustments to the weight values, this algorithm had excellent adaptability. This review provides a reference for further research on path planning algorithms in the process of smart parking. [ABSTRACT FROM AUTHOR]

To solve the problems of poor surface quality and low tool life in conventional machining (CM) of AerMet100 steel, an experimental study was conducted in laser-assisted machining (LAM) of AerMet100 steel. The effects of laser power, cutting speed, feed rate, and depth of cut on the surface roughness of AerMet100 steel were studied based on a single-factor experiment. The degree of influence of each factor on the surface roughness was evaluated by analyses of variance and range in the orthogonal experiment, and the combination of process parameters for the optimal surface roughness was obtained. The order of influence was as follows: laser power > cutting speed > depth of cut > feed rate; the optimal combination of process parameters was laser power 200 W, cutting speed 56.5 m/min, feed rate 0.018 mm/rev, and depth of cut 0.3 mm. Compared to CM, the surface morphology of the workpiece under the optimization of LAM was relatively smooth and flat, the surface roughness Ra was 0.402 μm, which was reduced by 62.11%, the flank wear was reduced from 208.69 μm to 52.17 μm, there were no tipping or notches, and the tool life was significantly improved. The study shows that the LAM of AerMet100 steel has obvious advantages in improving surface quality and reducing tool wear. [ABSTRACT FROM AUTHOR]

Aflatoxin B1 (AFB1) contamination is a serious threat to nutritional safety and public health. The CotA-laccase from Bacillus licheniformis ANSB821 previously reported by our laboratory showed great potential to degrade AFB1 without redox mediators. However, the use of this CotA-laccase to remove AFB1 in animal feed is limited because of its low catalytic efficiency and low expression level. In order to make better use of this excellent enzyme to effectively degrade AFB1, twelve mutants of CotA-laccase were constructed by site-directed mutagenesis. Among these mutants, E186A and E186R showed the best degradation ability of AFB1, with degradation ratios of 82.2% and 91.8% within 12 h, which were 1.6- and 1.8-times higher than those of the wild-type CotA-laccase, respectively. The catalytic efficiencies (kcat/Km) of E186A and E186R were found to be 1.8- and 3.2-times higher, respectively, than those of the wild-type CotA-laccase. Then the expression vectors pPICZαA-N-E186A and pPICZαA-N-E186R with an optimized signal peptide were constructed and transformed into Pichia pastoris GS115. The optimized signal peptide improved the secretory expressions of E186A and E186R in P. pastoris GS115. Collectively, the current study provided ideal candidate CotA-laccase mutants for AFB1 detoxification in food and animal feed and a feasible protocol, which was desperately needed for the industrial production of CotA-laccases. [ABSTRACT FROM AUTHOR]

Orchid flowers have evolved in concert with pollinators to form highly specialized structures resulting in zygomorphy. In dicotyledons, it is widely accepted that CYC-like genes are involved in the dorsoventral polarity establishment of flowers, which determines the development of zygomorphic flowers. However, the function of TCP transcription factors involved in orchid floral development is rarely known. Here, we found 15 unigenes with TCP domain (EpTCPs) from the previously reported Erycina pusilla unigene database. The expression patterns of EpTCPs in various tissues and different floral organs were successively detected by quantitative real-time PCR. The results revealed that the CYC-like gene (EpTCP25) and CIN-like genes (EpTCP11 and EpTCP26) were highly expressed in inflorescences but lowly expressed in leaves and roots. What is more, these three genes were expressed relatively high in the dorsal labellum, and EpTCP26 showed differential expression along the dorsoventral polarity of tepals, which was high in the dorsal and low in the ventral. Ectopic expression of EpTCP25 in Arabidopsis repressed primary root growth and delayed flowering. EpTCP26 overexpression in Arabidopsis promoted primary root growth and leaf growth. In contrast, EpTCP11 overexpression repressed primary root growth and changed the radially symmetric flower to a bilaterally symmetric flower by inhibiting the elongation of one or two adjacent petals. In addition, the homeotic transition of floral organs is generated when these genes are ectopically expressed in Arabidopsis, suggesting their roles in floral morphogenesis. Altogether, our results indicate that CIN-like genes would be associated with the unique flower pattern development of Erycina pusilla. [ABSTRACT FROM AUTHOR]

The crown closure of Platycladus orientalis forests has a wide-ranging impact on vegetation and soil, thereby affecting the overall functioning of the ecosystem. There is limited research on the effects of the Platycladus orientalis forest crown closure on changes in community plant functional traits, and their interactions are not yet clear. Therefore, we investigated 50 plots of different types of Platycladus orientalis crown closure, and we measured the functional traits of nine shrub species and 68 herb species in 50 plots under five different densities of Platycladus orientalis forests in the Loess Plateau. The consequence of Pearson's correlation analysis showed significant positive correlations between LC and LTD, LN and LP, LN and LNP, LN and LV, LN and H, LP and LV, LP and H, and SLA and LV (p < 0.05). LC was significantly negatively correlated with LP, LC with SLA, LC with LV, LN with LTD, LP with LNP, LP with LTD, and LTD with H (p < 0.05). Only the soil phosphorus content (SP) and soil water content (SWC) showed a significant positive correlation with multiple plant functional traits. The crown closure of Platycladus orientalis forests increased significantly, as did the plant functional features. Changes in the Platycladus orientalis forest crown closure significantly increased the LC, LV, LN, LP, and SLA in plant functional traits. An increase in Platycladus orientalis forest crown closure significantly increased the soil organic carbon (SC), soil phosphorus content (SP), soil nitrogen content (SN), soil water content (SWC), field capacity (FC), and soil porosity (PO). Based on a structural equation model, we found that, while changes in the Platycladus orientalis forest crown closure did not directly affect plant functional traits, they could indirectly influence these traits through soil factors, primarily the soil water content (SWC) and soil phosphorus content (SP) (p < 0.05). Additionally, the mechanisms of the Platycladus orientalis forest crown closure's impact on different functional traits vary. The research results provide scientific elements for the ecological restoration of Platycladus orientalis forests on the Loess Plateau. [ABSTRACT FROM AUTHOR]

Paclitaxel, a microtubule-stabilizing chemotherapy drug, can cause severe paclitaxel-induced peripheral neuropathic pain (PIPNP). The roles of transient receptor potential (TRP) ion channel vanilloid 1 (TRPV1, a nociceptor and heat sensor) and melastatin 8 (TRPM8, a cold sensor) in PIPNP remain controversial. In this study, Western blotting, immunofluorescence staining, and calcium imaging revealed that the expression and functional activity of TRPV1 were upregulated in rat dorsal root ganglion (DRG) neurons in PIPNP. Behavioral assessments using the von Frey and brush tests demonstrated that mechanical hyperalgesia in PIPNP was significantly inhibited by intraperitoneal or intrathecal administration of the TRPV1 antagonist capsazepine, indicating that TRPV1 played a key role in PIPNP. Conversely, the expression of TRPM8 protein decreased and its channel activity was reduced in DRG neurons. Furthermore, activation of TRPM8 via topical application of menthol or intrathecal injection of WS-12 attenuated the mechanical pain. Mechanistically, the TRPV1 activity triggered by capsaicin (a TRPV1 agonist) was reduced after menthol application in cultured DRG neurons, especially in the paclitaxel-treated group. These findings showed that upregulation of TRPV1 and inhibition of TRPM8 are involved in the generation of PIPNP, and they suggested that inhibition of TRPV1 function in DRG neurons via activation of TRPM8 might underlie the analgesic effects of menthol. [ABSTRACT FROM AUTHOR]

The random movement of occupants in a high-speed railway station results in a more complex indoor environment. In this study, the indoor thermal environment and the thermal comfort in summer were investigated via field measurements and questionnaires in the waiting hall of a high-speed railway station. The results showed that there was an uneven horizontal temperature distribution in the area, and over 30% of the passengers were dissatisfied with the air conditioning system. In order to improve the control of the indoor temperature as well as reduce the energy consumption of the air conditioning system, an improved zonal control strategy and AMPC control optimization algorithm based on real-time people are proposed, and different control strategies are modeled and simulated using MATLAB/Simulink. It is concluded that the improved zonal control method proposed in this paper can save 28.04% of the fan energy consumption compared with the traditional control strategy. [ABSTRACT FROM AUTHOR]

304 stainless steel demonstrates a low thermal conductivity and work hardening characteristics, resulting in its processing, and will adhere to the tip of the tool; as well as the phenomenon of chipping, shortening the life of the tool. AlTiN coatings are representative of coatings applied to carbide tools. In this paper, AlTiN coatings with different arc current processes were deposited on carbide milling inserts using arc ion plating. The microstructure, mechanical properties, and milling properties of the coatings were investigated by using the SEM, EDS, XRD, scratch meter, friction and wear meter, and vertical machining center. The findings revealed that all coatings displayed columnar crystal growth, free from cracks and voids. With an increasing arc current, there was a notable increase in surface droplets, pits, and coating thickness. The coating deposited at a 140 A arc current showed a pronounced (200) orientation preference. The adhesion force peaked at 56.0 N with a subsequent decline, and the friction coefficient hit its lowest point of 0.429 at 140 A, contrasting with its highest value of 0.55 at 160 A. After 39 min of dry milling, the tool with a 140 A AlTiN coating exhibited minimal wear of 0.196 mm, just below the 0.2 mm failure threshold, indicating superior performance at this arc current setting. [ABSTRACT FROM AUTHOR]

In highway reconstruction and expansion projects, the impact of the abandoned dreg body produced by the original highway on the new bridge project cannot be ignored. If the abandoned dregs are not handled properly, they will cause great threat to the operation of the new highway as well as to the safety of the surrounding environment and personnel. In this paper, based on a South China highway reconstruction and expansion project, a model of the abandoned dreg body, bridge pile and anti-slide pile is established with the help of finite difference software FLAC3D 5.0. The calculation results show that compared with burying the anti-slide pile, slope cutting is more effective for improving the stability of the abandoned dreg body. Under the action of vertical force intensity, the displacement of the top of the pile increases continuously, and the maximum bending moment of the pile body increases firstly and then decreases. The pile displacement and bending moment of the bridge pile increase significantly in the storm condition compared with the normal condition. In addition, the abandoned dreg body hazard prevention and control technical measures are studied. The impacts of different locations of anti-slide pile burial on bridge piles are analyzed and compared, and it is determined that the optimal location of anti-slide pile burial is the middle of the secondary slope. [ABSTRACT FROM AUTHOR]

With the acceleration of transportation infrastructure and the densification of transportation networks, there has been an increase in bridge pile construction near oil and gas pipelines. Selecting bridge pile construction methods with minimal impact and reducing the adverse effects of bridge pile construction on nearby oil and gas pipelines are of great importance. This paper uses FLAC3D 6.0 software to simulate and analyze the impact of two different pile construction methods, rotary drilling and impact drilling, on adjacent oil pipelines. The results show that the horizontal displacement of oil pipelines during rotary drilling construction is nearly 90% lower than that of the traditional impact drilling method, and the axial stress is reduced by nearly 85%. Furthermore, numerical simulations of rotary drilling under different conditions were conducted to analyze and summarize the patterns of how different conditions affect construction vibration and stress. This study provides a reference for bridge pile construction near oil and gas pipelines or important buildings. [ABSTRACT FROM AUTHOR]

Upward comparisons are prevalent in life and have a significant influence on consumer psychology and subsequent behavior. Previous research examined the effects of upward comparisons on consumption behavior, mainly focusing on behavior that evokes positive emotions (e.g., donation behavior, sustainable consumption) or behavior that evokes negative emotions (e.g., impulsive consumption, compulsive consumption) and less on behavior that evokes both negative emotions and positive emotions (i.e., counterhedonic consumption). This research examined the effect of upward comparisons on counterhedonic consumption. Five studies (N = 1111) demonstrated that upward comparison (vs. non-upward comparison) leads to counterhedonic consumption, and this effect is mediated by relative deprivation (Studies 2 and 3). In addition, this research showed that the comparison targets moderate the effects of upward comparisons on counterhedonic consumption. Specifically, when the comparison target is a friend, an upward comparison (vs. non-upward comparison) leads to counterhedonic consumption. When the comparison target is a stranger, an upward comparison (vs. non-upward comparison) has no significant influence on counterhedonic consumption (Study 5). Our findings extend the research on upward comparisons, relative deprivation, and counterhedonic consumption. [ABSTRACT FROM AUTHOR]

The magnetization mechanism of Co-doped BaTiO3 ultrathin films is a subject of debate, which results in difficulties with the design of new multiferroics based on BaTiO3 matrixes. With the aid of a first-principles approach, it was observed that when the interstitial site and Ti vacancy were filled with Co, the configuration behaved in a nonmagnetic manner, indicating the significance of the Co content. Moreover, in the case of Co substituting two neighboring Ti atoms, when a direct current field was applied in the [100] direction, the magnetic domains excluding those in the [100], [010], and [001] directions were directed away. Further, the magnetoelectric constant was evaluated at ~449.3 mV/cmOe, showing strong magnetoelectric coupling at room temperature. Clearly, our study indicates that strict control of Ba, Ti, O, and Co stoichiometry can induce an electric and magnetic field conversion in two-dimensional BaTiO3 and may provide a new candidate for single-phase multiferroics for application in next-generation multifunctional devices. [ABSTRACT FROM AUTHOR]

With the increasing uncertainty of urban development, urban shrinkage in the rapid urbanization process in China has become increasingly serious. While many studies have explored urban shrinkage from the economic and population perspectives, they often ignore the essence of the phased evolution of economic and population factors. Thus, this study introduces the theory of economic resilience into the field of urban shrinkage and constructs a theoretical method for identifying urban shrinkage by integrating economic resilience and population change to reveal the evolutionary trajectory of regional urban growth and shrinkage. The results show that urban economic resilience and population change in the Shanxi-Shaanxi-Inner Mongolia region (SSIMR) exhibit strong volatility, highlighting the importance of conducting urban shrinkage studies within specific crisis disturbance scenarios. In the context of the "new normal of the economy", the economic resilience of cities in the SSIMR has significantly declined, and the problem of economic recession is gradually intensifying. The population change trend of cities in the SSIMR is relatively stable, with population loss being a common problem in urban development in the area and its scope and intensity increasing daily. Urban development in the SSIMR is evolving from global growth to widespread shrinkage, with 56.67% of the cities experiencing relative shrinkage, showing a spatial pattern of "western growth–eastern shrinkage". Factors such as the agglomeration effect, industrial structure, and policy system collectively shape the evolution of urban growth and shrinkage. [ABSTRACT FROM AUTHOR]

Deoxynivalenol (DON), primarily generated by Fusarium species, often exists in agricultural products. It can be transformed to 3-epi-deoxynivalenol (3-epi-DON), with a relatively low toxicity, via two steps. DDH in Pelagibacterium halotolerans ANSP101 was proved to convert DON to 3-keto-deoxynivalenol (3-keto-DON). In the present research, AKR4, a NADPH-dependent aldo/keto reductase from P. halotolerans ANSP101, was identified to be capable of converting 3-keto-DON into 3-epi-DON. Our results demonstrated that AKR4 is clearly a NADPH-dependent enzyme, for its utilization of NADPH is higher than that of NADH. AKR4 functions at a range of pH 5–10 and temperatures of 20–60 °C. AKR4 is able to degrade 89% of 3-keto-DON in 90 min at pH 7 and 50 °C with NADPH as the cofactor. The discovery of AKR4, serving as an enzyme involved in the final step in DON degradation, might provide an option for the final detoxification of DON in food and feed. [ABSTRACT FROM AUTHOR]

The identification and control of tire side slip angle is the key to vehicle stability control. Intelligent tire technology based on the sensing of side-slip acceleration inside the tire provides a novel method for estimating the tire side-slip angle. This study proposed a method to estimate the tire side-slip angle by using the frequency domain lateral acceleration of the tire. First, an intelligent tire testing system was constructed by independently developing a special rim assembly and data collector. A three-axis accelerometer was placed on the right side of the tire, and the acceleration value was acquired by using a wired method with a sampling frequency of 50 kHz. Second, based on the constructed test system, a tire side deflection test was carried out on the Flat Trac bench. Through data analysis, it was found that the lateral acceleration was in the frequency domain of 400 Hz. As the side-slip angle increased from −4° to 4°, the vibration amplitude gradually decreased. Moreover, the vibration amplitude within 0.5~2 kHz was highly correlated with the side-slip angle. Subsequently, the vibration amplitude of the lateral acceleration within 2 kHz was extracted at an interval of 20 Hz as the feature point, and a frequency domain data set FDAy3 was established together with the vertical load and tire pressure. Finally, the support vector machine (SVM) algorithm was employed to make predictions on the data set. The grid search method was utilized to find the optimal parameter values of the model penalty factor c and radial basis kernel function coefficient g, which were 1.4142 and 0.0884, respectively. The results suggested that the root mean square error of the model prediction was 0.0806°, and the maximum estimated angle deviation of the prediction was 0.4587°. Meanwhile, the optimal prediction accuracy and real-time performance were achieved when the number of feature points and the feature frequency band were 25 and within 500 Hz, respectively. The findings of this study confirm that it is feasible to estimate the tire side-slip angle based on the frequency domain lateral acceleration of the tire, which provides a new method for tire side-slip angle estimation. [ABSTRACT FROM AUTHOR]

As single-mode-fiber transmission systems are reaching their capacity limits, spatial-division multiplexing (SDM) techniques have been investigated to increase the per-fiber capacity. However, the compatibility with current single-mode transponders severely hinders the near-term deployment of SDM systems. In this paper, we experimentally propose two real-time SDM transmission schemes using commercial single-mode 400 G dual-polarized 16 quadrature amplitude modulation equipment. In the first experiment, 60 km weakly coupled single-mode 7-core fiber with a pair of fan-in and fan-out devices are adopted. In the second experiment, the fiber link consists of 60 km/150 km weakly coupled few-mode fiber (FMF) and low-modal-crosstalk mode multiplexers, in which only non-degenerate LP01 and LP02 modes are utilized. In order to investigate the effect of splice on SDM fiber links, 20-roll, 3 km multicore fibers (MCFs) and FMFs are spliced and tested in the experiments. The bit error rates of all SDM experiments are all below 4.75 × 10−2 forward-error-correction threshold of the 400 G transponders. The experimental results prove that the near-term deployment of SDM systems could be accelerated by utilizing weakly coupled MCFs or non-degenerate modes of weakly coupled FMFs which are compatible with commercial single-mode transponders without any software or hardware modifications. [ABSTRACT FROM AUTHOR]

Since its inception, induced pluripotent stem cell (iPSC) technology has been hailed as a powerful tool for comprehending disease etiology and advancing drug screening across various domains. While earlier iPSC-based disease modeling and drug assessment primarily operated at the cellular level, recent years have witnessed a significant shift towards organoid-based investigations. Organoids derived from iPSCs offer distinct advantages, particularly in enabling the observation of disease progression and drug metabolism in an in vivo-like environment, surpassing the capabilities of iPSC-derived cells. Furthermore, iPSC-based cell therapy has emerged as a focal point of clinical interest. In this review, we provide an extensive overview of non-integrative reprogramming methods that have evolved since the inception of iPSC technology. We also deliver a comprehensive examination of iPSC-derived organoids, spanning the realms of the nervous system, cardiovascular system, and oncology, as well as systematically elucidate recent advancements in iPSC-related cell therapies. [ABSTRACT FROM AUTHOR]

Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin found in many agricultural products and can cause reproductive disorders, mainly affecting spermatogenesis in male animals. Rutin (RUT) is a natural flavonoid compound recognized for its significant antioxidant, anti-inflammatory and estrogenic properties. The present study aimed to determine the protective role of RUT against ZEN-induced reproductive toxicity in male mice. Twenty-four adult Kunming male mice were divided into four groups: control, RUT (500 mg/kg RUT), ZEN (10 mg/kg ZEN), ZEN + RUT (500 mg/kg RUT + 10 mg/kg ZEN), with six replicates per treatment. The results indicated that RUT mitigated ZEN-induced disruption in spermatogenic cell arrangement, decreased spermatozoa count, and increased sperm mortality in the testes. RUT significantly restored ZEN-induced reduction in T, FSH, LH, and E2 serum levels. Moreover, RUT mitigated ZEN-induced apoptosis by increasing the mRNA expression level of bcl-2, decreasing the mRNA expression level of kiss1-r, and decreasing the protein expression level of caspase 8 in reproductive tissues. These findings indicate the protective role of RUT against ZEN-induced reproductive toxicity in male mice by regulating gonadotropin and testosterone secretions to maintain normal spermatogenesis via the HPG axis, which may provide a new application direction for RUT as a therapeutic agent to mitigate ZEN-induced reproductive toxicity. [ABSTRACT FROM AUTHOR]

In the field of aircraft engine diagnostics, many advanced algorithms have been proposed over the last few years. However, there is still wide room for improvement, especially in the development of more integrated and complete engine health management systems to detect, identify, and forecast complex faults in a short time. Furthermore, it is necessary to ensure that these systems preserve their capabilities over time despite engine deterioration. This paper addresses these necessities by proposing an integrated system that considers the joint operation of feature extraction, anomaly detection, fault identification, and prognostic algorithms for engines with long operation times. To effectively reveal the actual engine condition, light adaptive degraded engine models are computed along with different health indicators that are used as inputs to train and test recognition and prediction models. The system is developed and evaluated using a specialized NASA platform which provides data from a turbofan engine fleet simultaneously experiencing long-term performance deterioration and faults. Contrary to other compared solutions, our results show that the proposed system is robust against the effects of engine deterioration, maintaining its level of detection, recognition, and prediction accuracy over a total engine service life. The low computational cost algorithms has generally fast performance in all stages, making the system suitable for online applications. [ABSTRACT FROM AUTHOR]

Thermal efficiency is one of the important indices used to evaluate the operational energy efficiency of hot blast stoves. In this study, a method for calculating the thermal efficiency of hot blast stoves was developed based on simulation results. The working process of top combustion hot blast stoves was numerically simulated through the established 3D fluid flow heat transfer model. The system thermal efficiency of hot blast stoves was calculated according to the simulation data, referring to the Chinese national standard, "measurement and calculation method of the heat balance of blast furnace hot blast stove" (GB/T 32287-2015). In particular, a "segmented calculation and accumulate by time" method was proposed based on the air supply curve to more precisely calculate the heat carried away by the hot blast. The results indicate that when the burning air supply cycles increased from 120 to 240 min, the thermal efficiency showed a trend of first decreasing and then increasing, with the value ranging between 70.39% and 72.48%. The reason for the decrease in thermal efficiency at a burning cycle of 150 min is explained based on heat transfer theory combined with the structural characteristics of hot blast stoves. This study provides a convenient and effective method for calculating the thermal efficiency of hot blast stoves, which helps us to evaluate and improve the operating process of hot blast stoves. [ABSTRACT FROM AUTHOR]

Cottonseed meal (CSM) is the major by-product of the cottonseed oil extraction process with high protein content. However, the presence of free gossypol (FG) in CSM severely restricts its utilization in the food and animal feed industries. The development of a biological strategy for the effective removal of FG in CSM has become an urgent need. In this study, three bacterial laccases including CotA from Bacillus licheniformis, CueO from Escherichia coli, and LcLac from Loigolactobacillus coryniformis were heterologously expressed and investigated for their FG degradation ability. The results showed that CotA laccase displayed the highest FG-degrading capacity among the three laccases, achieving 100% FG degradation at 37 °C and pH 7.0 in 1 h without the addition of a redox mediator. Moreover, in vitro and in vivo studies confirmed that the hepatotoxicity of FG was effectively eliminated after oxidative degradation by CotA laccase. Furthermore, the addition of CotA laccase could achieve 87% to 98% FG degradation in defatted CSM within 2 h. In conclusion, CotA laccase can be developed as an effective biocatalyst for the detoxification of FG in CSM. [ABSTRACT FROM AUTHOR]

To better analyze the fluctuation characteristics and development law of tunnel deformation data, multifractal theory is applied to tunnel deformation analysis. That is, the multifractal detrended fluctuation analysis (MF-DFA) model is first utilized to carry out the multifractal characterization of tunnel deformation data. Further, Mann–Kendall (M–K) analysis is utilized to construct the dual criterion (∆α indicator criterion and ∆f(α) indicator criterion) for the tunnel deformation early warning study. In addition, the particle swarm optimization long-short-term memory (PSO-LSTM) prediction model is used for predicting tunnel settlement. The results show that, in reference to the tunnel warning level criteria and based on the Z-value results of the indicator criterion, the warning level of all four sections is class II. At the same time, through the analysis of tunnel settlement predictions, the PSO-LSTM model has a better prediction effect and stability for tunnel settlement. The predicted results show a slow increase in tunnel settlement over the next 5 days. Finally, the tunnel warning level and the predicted results of tunnel settlement are analyzed in a comprehensive manner. The deformation will increase slowly in the future. Therefore, monitoring and measurement should be strengthened, and disaster preparedness plans should be prepared. [ABSTRACT FROM AUTHOR]

Prostatitis, a prevalent urinary tract disorder in males, has a complex etiology that leads to severe clinical discomfort. Pule'an Tablets, a classic single-component formulation primarily based on rapeseed pollen, have been clinically proven to have a beneficial therapeutic effect on both prostatitis and benign prostatic hyperplasia. However, there is currently a lack of research on the chemical composition and mechanisms of action of Pule'an Tablets in treating prostatitis. In this study, using liquid chromatography–mass spectrometry (LC-MS), a total of 53 compounds in Pule'an Tablets were identified, including flavonoids, phenylpropionamides, lipids, glucosinolates, and nucleic acids. Subsequently, through a network pharmacology analysis, potential target genes and their mechanisms of action were predicted accordingly. The results suggested that genes such as LPAR5, LPAR6, LPAR4, LPAR3, LPAR2, LPAR1, F2, ENPP2, MMP9, and TNF, along with pathways like prostate cancer, endocrine resistance, bladder cancer, and the IL-17 signaling pathway, may represent potential pathways involved in the therapeutic effects of Pule'an Tablets. This study represents the first systematic investigation into the chemical composition of Pule'an Tablets, shedding light on the potential mechanisms underlying their efficacy in treating prostatitis. These findings could serve as a valuable reference for future pharmacological research on Pule'an Tablets. [ABSTRACT FROM AUTHOR]

With the development of infrastructure construction in mountainous areas, the number of new extra-long tunnels is increasing. However, these tunnels often face the challenge of complex and variable surrounding rock grades, resulting in a large number of overbreak and underbreak due to the untimely adjustment of smooth blasting parameters. This study focuses on the optimization of the peripheral hole charging structure and blasting parameters for extra-long hard rock tunnels, aiming to improve the effectiveness of smooth blasting technology. The results of this study demonstrate a significant improvement in the effect of smooth blasting after implementing bidirectional polymerization blasting in the tunnel. A comparison between the bidirectional shaped charge and spaced decoupled charge blasting reveals that the former yields better results. To obtain accurate data on the tunnel section profile during excavation, a laser cross-section meter is used for measurement. Furthermore, this study quantitatively compares the optimization effect of smooth blasting parameters. The multifractal characteristics of the tunnel profile overbreak point sequences are analyzed under different smooth blasting schemes using the multifractal detrended fluctuation analysis (MF-DFA) method. It is found that both the spaced decoupled charge and the bidirectional shaped charge blasting exhibit multifractal features in the overbreak measurement point sequences. The calculation results of the multifractal features of the tunnel profile under different smooth blasting plans are in line with the actual situation. [ABSTRACT FROM AUTHOR]

Microglia are resident innate immune cells that play an essential role in the development and surveillance of the central nervous system as well as the shared pathogenesis of neurodegenerative diseases. Microglia rapidly respond to multiple inflammatory stimuli and activate towards different phenotypes, such as pro-inflammatory and anti-inflammatory phenotypes. Cytokines, epigenetic and long non-coding RNA modulations have been shown to regulate microglial activation; however, the role of circRNAs in microglia-mediated neuroinflammation remains elusive. Here, we performed circRNA sequencing in IL-4-treated anti-inflammatory microglia and discovered 120 differentially expressed circRNAs. We systemically verified the identities of circRNAs by assays of PCR, RNase R treatment and fluorescent in situ hybridization (FISH), among others. We found that circAdgre1 promoted IL-4-induced anti-inflammatory responses and further conferred neuroprotective effects upon lipopolysaccharide (LPS) stimuli. Taken together, our results show that circRNAs might be possible therapeutic targets for microglia-mediated neuroinflammation and neurodegenerative diseases. [ABSTRACT FROM AUTHOR]

Hydrogenation stands out as one of the most promising techniques for converting biomass-derived molecules into valuable products. The expected products of upgrading biomass molecules include hydrocarbon, oxygenate, and methane. Ni-based catalysts have attracted considerable interest owing to their unique properties and relatively low cost. In this work, NiO prepared by the calcination and urea precipitation methods, namely NiO–C and NiO–U, is investigated for the hydrogenation of anisole. It is found that reaction temperature exerts a significant influence on the hydrogenation pathways. At 150 °C, C–reduced NiO proves more inclined towards hydrogenation, while U–reduced NiO demonstrates a tendency for hydrodeoxygenation (HDO). Moreover, as the temperature rises, both nickel catalysts change the reaction route to hydrogenolysis and eventually only produce methane at 300 °C, whereas metallic Ni is formed as the catalytic active phase. In situ FTIR experiments suggest the hydrogenolysis pathway and the formation of methane. This work investigates a route to produce methane from biomass molecules. [ABSTRACT FROM AUTHOR]

This paper presents an experimental study on the compressive performance of longitudinal steel-fiber-reinforced polymer composite bars (SFCBs) in concrete cylinders confined by different type of fiber-reinforced polymer (FRP) composites. Three types of concrete cylinders reinforced with (or without) longitudinal SFCBs and different transverse FRP confinements were tested under monotonic compression. The results showed that the post-yield stiffness of SFCBs is higher when confined with high elastic modulus carbon fiber-reinforced polymer (CFRP) composite than with low elastic modulus basalt fiber-reinforced polymer (BFRP) composite. Decreasing confinement spacing did not significantly improve the compressive strength of SFCBs in concrete cylinders. The compressive failure strain of SFCBs could possibly reach 88% of its tensile peak strain in concrete cylinders confined by CFRP sheets, which is significantly higher than the value (around 50%) in previous studies. Existing design equations, which applied a strength reduction factor or a maximum compressive strain of concrete to consider the compressive contributions of SFCBs in concrete members, underestimate the load-carrying capacity of SFCB-reinforced concrete cylinders. The design equation that considers the actual compressive stress of SFCBs gives the most accurate prediction; however, its applicability and accuracy need to be verified with more experimental data. [ABSTRACT FROM AUTHOR]

Hydraulic fracturing is an indispensable technology for the development of shale oil and shale gas. Knowing the changes in the rock mechanical properties and failure modes during hydraulic fracturing is the key to improving the efficiency of hydraulic fracturing. Based on experiments and simulations, it can be concluded that the injection of fracturing fluid in the hydraulic fracturing caused deformation of the fracture surface, and the rock mechanical properties experienced degradation with a maximum reduction in the rock mechanical properties of 44.24%. As indicated in the experiments, the displacement of the measurement point was decreased with the distance increase between the injection point and the measurement point. According to the numerical simulations, tensile failure is the main failure mode in hydraulic fracturing, but the percentage of shear failure had an obvious increase with the increase in distance between the injection point and the measurement point. Comparing DDS #1 and DDS #5, the DDS #5 measurement point was farther away from the injection point, and the average percentage of shear failure increased from 21.94 to 52.72%. Meanwhile, the increase in the branch fractures also caused shear failure to occur. Comparing Sample 1 and Sample 3, in Sample 3, which had more branch fractures, the average percentage of shear failure increased from 33.12 to 37.58%. Due to the porous medium of the reservoir rock, the enormous pressure generated during the injection of fracturing fluid caused significant deformation of the fracture surface, leading to the tensile failure of the rock. The displacement of the fracture surface caused by the fracturing fluid injection also led to the deformation of the pore throat structure; thus, the shear failure increased when the measurement point was away from the injection point. [ABSTRACT FROM AUTHOR]

Mineral admixtures (MA), like fly ash (FA), silica fume (SF), and slag (S), are usually added to cement-based materials to improve their compactness and further enhance their mechanical properties, permeability resistance, and durability. In this study, low-field nuclear magnetic resonance (LF-NMR) is adopted to explore the evolution of the early hydration characterization of cement-based materials with MA by testing the transverse relaxation time T2. Meanwhile, the effect of MA on mechanical properties is analyzed by measuring compressive and flexural strength. The results show that, in the early hydration (0–7 days), the T2 distribution shows a trend of gradually moving to a short relaxation time and changes from a double peak to a single main peak. The decrease in T2i (main peak vertex) means that the evaporated water is gradually distributed in smaller pores with more motion constraints. However, the type and content of MA have little effect on T2i. Porosity gradually decreases in the period of early hydration. The addition of MA causes the porosity to decrease, and the order influence is FA > S > SF, i.e., the porosities of cement paste with 0%MA, 10%FA + 10%SF, 10%FA + 10%S, and 20%FA at 7 days are 48%, 44.5%, 40.7%, and 40.2%, respectively. Additionally, the addition of MA to cement-based materials also decreases the early strength, and the influence order is FA > S > SF, i.e., the compression strength values of cement paste with 0%MA, 10%FA + 10%SF, 10%FA + 10%S, and 20%FA at 7 days are 47.8 MPa, 40.1 MPa, 38.6 MPa, and 37 MPa, respectively. [ABSTRACT FROM AUTHOR]

Due to the excellent properties of carbon fiber-reinforced polymers (CFRPs), such as high strength and strong corrosion resistance, the traditional water-jet-guided laser (WJGL) technology has problems with fiber pull-out and has a small cutting depth when processing CFRPs. Therefore, in this study, we used high-power water-jet-guided laser (HPWJGL) technology to perform groove processing experiments on CFRPs. The effects of four key process parameters, high laser power, pulse frequency, feed rate, and water-jet pressure, on the cutting depth were investigated by a single-factor experiment. The formation mechanism of groove cross-section morphology and the processing advantages of high-power water-jet-guided lasers were analyzed. On this basis, the mathematical prediction model of cutting depth was established by using the response surface method (RSM), and the optimal combination of process parameters was obtained. The mathematical prediction model was verified by experiments, and the error was only 1.84%, indicating that the model had a high reference value. This study provides a reference for the precision machining of HPWJGL technology. [ABSTRACT FROM AUTHOR]

Southwest China is an ecologically fragile area with a high proportion of karstland and large variations in terrain, and it can be greatly affected by land use change. From 2000 to 2020, with the acceleration of urbanization in the whole country, the macro-scale Grain-for-Green Program (GFGP) has been developed in the karstland of southwest China. This has resulted in the expansion of forest and construction land with a reduction in cultivated land. The response of ecosystem services (ESs) to these changes needs to be investigated. However, there is a lack of in-depth analysis of the karstland of southwest China, and current studies mostly focus on the spatio-temporal variation in individual ESs or how the whole land use change affects ESs. Thus, our study uses an InVEST model and geographically and temporally weighted regression (GTWR) to examine the spatio-temporal variation in land use associated with four ESs, namely water conservancy (WC), soil conservancy (SC), carbon sequestration (CS), and habitat quality (HQ). We found that the GFGP area first increased and then decreased, aggregating to 4.48 × 104 km2 from 2000 to 2020. We also observed that from 2000 to 2020, ESs remained stable or gradually increased (despite fluctuations), SC was the most stable, whereas WC, CS, and HQ first decreased and then fluctuated more. This may be related to the destruction of topsoil in the early stages of the GFGP and the relatively weak ES supply capacity of the young trees. Moreover, the response of ecosystem services to the GFGP was spatially heterogeneous, suggesting a strong driving influence of the local environment, mainly caused by the distribution of karstland and terrain, differentiated urbanization levels, and the intensity of the GFGP. Specifically, the relatively significantly positive effects of WC, SC, and CS were found in western and northern cities, and so was the HQ in southeastern cities. On the contrary, the negative impacts of urbanization were found to weaken over time, suggesting the trade-off effect of the GFGP. Our findings would contribute to the development of effective forest management strategies and provide valuable insights for policymakers and stakeholders involved in ecosystem restoration and conservation efforts by exploring the impacts of the GFGP and urbanization on ESs. [ABSTRACT FROM AUTHOR]

Plinabulin, a new antitumor drug developed from marine natural products that targets microtubules in cancer cells, is currently being tested in a phase III clinical study. Plinabulin has been clinically proven to be effective on leukopenia. However, to our knowledge, there are no reports investigating the pharmacokinetics of plinabulin in individuals with leukopenia and healthy individuals. In this study, we developed a rapid and sensitive UHPLC-MS/MS method for the detection of plinabulin for the first time. Using a novel cyclophosphamide-induced leukopenia model, we investigated the differences in the pharmacokinetic characteristics of plinabulin between rats with leukopenia and normal rats. Plinabulin and propranolol (IS) peaks were separated by gradient elution for a total run time of 5 min. The methodological validation showed a good accuracy (101.96–109.42%) and precision (RSD ≤ 5.37%) with the lower limit of quantification at 0.5 ng/mL. The recovery of plinabulin was between 91.99% and 109.75% (RSD ≤ 7.92%). The values of the area under the plasma concentration-time curve (AUC0-t) for leukopenia groups and control groups at doses of 0.5 mg/kg, 1 mg/kg, and 3 mg/kg were 148.89 ± 78.74 h·μg/L and 121.75 ± 31.56 h·μg/L; 318.15 ± 40.00 h·μg/L and 272.06 ± 42.85 h·μg/L; and 1432.43 ± 197.47 h·μg/L and 1337.12 ± 193.56 h·μg/L; respectively. The half-lives (t1/2s) of plinabulin were 0.49–0.72 h for leukopenia groups and 0.39–0.70 h for control groups at three doses, and the clearance rates (CLs) of plinabulin were 2.13–3.87 L/h/kg for leukopenia groups and 2.29–4.23 L/h/kg for control groups. Pharmacokinetic results showed that there was no significant pharmacokinetic difference between the normal group and the leukopenia group. Based on the power model, plinabulin exhibits a lack of dose proportionality over the dose range of 0.5–3 mg/kg after intravenous administration. This study provides guidance for the development of plinabulin as a potential candidate for the treatment of chemotherapy-induced leukopenia. [ABSTRACT FROM AUTHOR]

The Chinese government has continuously stressed the Chinese model and its characteristics for national economic governance. As a result, it is worth studying the effect of China's rule of law on the government's sustainable economic management, and the rule of law regarding this model can also provide a reference for governing other countries. This paper adopts the multiple linear regression analysis method using time-series data from 2007 to 2017 and from 2002 to 2019. This reflects the rule of law in the government's sustainable economic management via the impact on government institutions, people's courts, people's procuratorates, and social organizations on the economy. The results show that the economic effect of the rule of law on government sustainable economic management is not significant. The rule of law in fiscal revenue and social organizations, rather than being arbitrarily dictated by powerful people, positively affects the economy. This effect was seen only in the 2002–2019 time series, but not in the 2007–2017 time series. Based on these results, it is suggested that China should strengthen the rule of law in managing its courts and procuratorates to enhance their roles in ensuring and accelerating sustainable economic development and regulating government activities. That is, the economic management effect of the public sectors, which indirectly participate in economic governance, is insufficient. This and other insightful policy recommendations are suggested to assist the government and policymakers in more effective and efficient sustainable economic management. Therefore, the system and regulations regarding their governance should not only focus on economic effects but also pay attention to the sustainable effects of economic development. [ABSTRACT FROM AUTHOR]

The scoliosis report is a diagnosis made by the clinician looking at X-ray images of the spine. However, with numerous images, writing the report can be time-consuming and error-prone. Therefore, this paper proposes an automatic generation model of the end-to-end scoliosis Lenke classification report. The model automatically generates a short diagnostic text to explain the results of the classifiers' Lenke classification diagnosis of scoliosis. Instead of reproducing the original diagnostic report, the original diagnostic report is described as a short sentence with diagnostic significance. In the model, the CBAM attention module is added to the residual's path of ResNet-50 to extract key regional features of the image, and the improved Long Term and Short Term Memory Network (M-LSTM) fusion attention mechanism with additional gated operations is used as the decoder to generate more relevant description statements. The model was verified on the scoliosis dataset from Guizhou Orthopaedic Hospital, and the generated diagnostic text obtained good scores on BLEU and CIDEr evaluation indexes, and also satisfactory scores on the evaluation criteria of five professional clinicians. Therefore, the diagnostic text generated by this method had good performance in accuracy and semantic expression. [ABSTRACT FROM AUTHOR]

The study of human torso medical image segmentation is significant for computer-aided diagnosis of human examination, disease tracking, and disease prevention and treatment. In this paper, two application tasks are designed for torso medical images: the abdominal multi-organ segmentation task and the spine segmentation task. For this reason, this paper proposes a net-work model CI-UNet improve the accuracy of edge segmentation. CI-UNet is a U-shaped network structure consisting of encoding and decoding networks. Firstly, it replaces UNet's double convolutional backbone network with a VGG16 network loaded with Transfer Learning. It feeds image information from two adjacent layers in the VGG16 network into the decoding grid via information aggregation blocks. Secondly, Polarized Self-Attention is added at the decoding network and the hopping connection, which allows the network to focus on the compelling features of the image. Finally, the image information is decoded by convolution and Up-sampling several times to obtain the segmentation results. CI-UNet was tested in the abdominal multi-organ segmentation task using the Chaos (Combined CT-MR Healthy Abdominal Organ Segmentation) open challenge dataset and compared with UNet, Attention UNet, PSPNet, DeepLabv3+ prediction networks, and dedicated network for MRI images. The experimental results showed that the average intersegmental union (mIoU) and average pixel accuracy (mPA) of organ segmentation were 82.33% and 90.10%, respectively, higher than the above comparison network. Meanwhile, we used CI-UNet for the spine dataset of the Guizhou branch of Beijing Jishuitan Hospital. The average intersegmental union (mIoU) and average pixel accuracy (mPA) of organ segmentation were 87.97% and 93.48%, respectively, which were approved by the physicians for both tasks. [ABSTRACT FROM AUTHOR]

The demand for tunnel construction continues to grow by leaps and bounds. Therefore, tunnel mechanization construction is receiving more and more attention for improving excavation ergonomics. To enhance the scientific and comprehensive evaluation results of tunnel drilling and blasting method excavation ergonomics, a set of evaluation methods of tunnel drilling and blasting method excavation ergonomics based on the game theory G2-EW-TOPSIS model is proposed. From the three dimensions of drilling efficiency, construction process duration, and synergistic influence factors, a tunnel drilling and blasting construction ergonomics evaluation index system consisting of 11 indicators such as perimeter hole drilling efficiency, drilling duration, construction quality, and comprehensive cost is constructed. The subjective and objective weights of evaluation indicators are calculated by using the improved sequential relationship analysis method (G2 method) and entropy weight method, respectively, and the combination weights are carried out by using game theory method (GTM) with the Nash equilibrium as the goal. The indices are classified into five grades: excellent (I), good (II), average (III), rather poor (IV), and poor (V), according to the daily tunnel construction. The excavation ergonomics index to be evaluated is calculated using the combined weights, and the comprehensive evaluation index of excavation ergonomics to be evaluated is calculated using the technique for order preference by similarity to an ideal solution (TOPSIS). The proposed rating model was used to analyze the excavation ergonomics of the Shangtianling Tunnel in the Chizhou–Huangshan High-Speed Railway using jumbo drills (JD) and drilling machines (DM) in large- and small-mileage construction, respectively, and to obtain the excavation ergonomics rating and comprehensive evaluation rating of each evaluation object. The research results show that the established excavation ergonomics evaluation model can effectively identify the main factors affecting the excavation ergonomics of the drill and blast method, and has a certain reference value. [ABSTRACT FROM AUTHOR]

In order to automatically process ultra-fine copper tube electrodes, this study proposes a new method of ultrasonic vibration processing of ultra-fine copper tube, analyzes its processing principle, designs a new set of experimental processing equipment and completes the processing of 1.206 mm inner diameter, 1.276 mm outer diameter with core brass tube. Not only can the copper tube be completed with core decoring, the surface of the processed brass tube electrode also has good integrity. The effect of each machining parameter on the surface roughness of the electrode after machining was investigated by a single-factor experiment and the optimal machining effect was achieved under the conditions of machining gap 0.1 mm, ultrasonic amplitude 0.186 mm, table feed speed 6 mm/min, tube rotation speed 1000 r/min and reciprocating machining two times. The surface roughness was reduced from 1.21 μm before machining to 0.11 μm, and the residual pits, scratches and oxide layer on the surface were completely removed, which greatly improved the surface quality of the brass tube electrode and prolonged its service life. [ABSTRACT FROM AUTHOR]

The landslide early warning system (LEWS) relies on various models for data processing, prediction, forecasting, and warning level discrimination. The potential different programming implementations and dependencies of these models complicate the deployment and integration of LEWS. Moreover, the coupling between LEWS and models makes it hard to modify or replace models rapidly and dynamically according to changes in business requirements (such as updating the early warning business process, adjusting the model parameters, etc.). This paper proposes a framework for dynamic management and integration of models in LEWS by using WebAPIs and Docker to standardize model interfaces and facilitate model deployment, using Kubernetes and Istio to enable microservice architecture, dynamic scaling, and high availability of models, and using a model repository management system to manage and orchestrate model-related information and application processes. The results of applying this framework to a real LEWS demonstrate that our approach can support efficient deployment, management, and integration of models within the system. Furthermore, it provides a rapid and feasible implementation method for upgrading, expanding, and maintaining LEWS in response to changes in business requirements. [ABSTRACT FROM AUTHOR]

Autophagy is a highly conserved recycling process of eukaryotic cells that degrades protein aggregates or damaged organelles with the participation of autophagy-related proteins. Membrane bending is a key step in autophagosome membrane formation and nucleation. A variety of autophagy-related proteins (ATGs) are needed to sense and generate membrane curvature, which then complete the membrane remodeling process. The Atg1 complex, Atg2-Atg18 complex, Vps34 complex, Atg12-Atg5 conjugation system, Atg8-phosphatidylethanolamine conjugation system, and transmembrane protein Atg9 promote the production of autophagosomal membranes directly or indirectly through their specific structures to alter membrane curvature. There are three common mechanisms to explain the change in membrane curvature. For example, the BAR domain of Bif-1 senses and tethers Atg9 vesicles to change the membrane curvature of the isolation membrane (IM), and the Atg9 vesicles are reported as a source of the IM in the autophagy process. The amphiphilic helix of Bif-1 inserts directly into the phospholipid bilayer, causing membrane asymmetry, and thus changing the membrane curvature of the IM. Atg2 forms a pathway for lipid transport from the endoplasmic reticulum to the IM, and this pathway also contributes to the formation of the IM. In this review, we introduce the phenomena and causes of membrane curvature changes in the process of macroautophagy, and the mechanisms of ATGs in membrane curvature and autophagosome membrane formation. [ABSTRACT FROM AUTHOR]