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Grasping and object manipulation have been considered key domains of Cyber-Physical Systems (CPS) since the beginning of automation, as they are the most common interactions between systems, or a system and its environment. As the demand for automation is spreading to increasingly complex fields of industry, smart tools with sensors and internal decision-making become necessities. CPS, such as robots and smart autonomous machinery, have been introduced in the meat industry in recent decades; however, the natural diversity of animals, potential anatomical disorders and soft, slippery animal tissues require the use of a wide range of sensors, software and intelligent tools. This paper presents the development of a smart robotic gripper for deployment in the meat industry. A comprehensive review of the available robotic grippers employed in the sector is presented along with the relevant recent research projects. Based on the identified needs, a new mechatronic design and early development process of the smart gripper is described. The integrated force sensing method based on strain measurement and magnetic encoders is described, including the adjacent laboratory and on-site tests. Furthermore, a combined slip detection system is presented, which relies on an optical flow-based image processing algorithm using the video feed of a built-in endoscopic camera. Basic user tests and application assessments are presented. [ABSTRACT FROM AUTHOR]

Given the increasing popularity of CrossFit® as a high-intensity functional training program and the potential benefits of citrulline malate (CM) in enhancing exercise performance through its role as a precursor to L-arginine and nitric oxide production, this study aimed to investigate the acute effects of CM supplementation on CrossFit® performance and cardiovascular function. Using a randomized, double-blind, placebo-controlled, cross-over design, 21 recreationally active participants (mean age 22.2 ± 2.6 years, mean body weight 75.9 ± 10.4 kg) with CrossFit® experience completed the "Cindy" workout under CM and placebo conditions. Participants consumed 4.4 g of CM or a placebo 60 min before the workout, and the performance was measured by the number of rounds completed. Secondary outcomes included heart rate response, time spent in different heart rate intensity zones, and post-exercise recovery time. The results indicated no significant difference in the number of rounds completed between the CM and placebo conditions (13.5 ± 5.2 vs. 13.8 ± 6.7 rounds, respectively; p = 0.587). However, the time spent in zone 4 (80–90% of HR max) was significantly increased in the CM condition (527 ± 395 s vs. 453 ± 334 s; p = 0.017), suggesting a potential benefit for aerobic capacity and anaerobic threshold. No significant differences in post-exercise recovery time were observed (6.6 ± 4.7 h vs. 6.9 ± 4.7 h; p = 0.475). This study highlights the need for further research with larger sample sizes, both genders, and different CM dosages to clarify these findings and better understand CM's role in enhancing athletic performance. [ABSTRACT FROM AUTHOR]

Sour cherry is a non-climacteric fruit that quickly loses its quality after harvest, so effective storage and packaging are essential to minimize postharvest decay. Our study aimed to (i) evaluate fruit decay incidence during shelf-life for both freshly harvested and six-week-cold-stored sour cherry fruits, comparing normal atmospheric conditions with modified atmosphere packaging across three sour cherry cultivars ('Érdi bőtermő', 'Újfehértói fürtös', and 'Petri'); and (ii) assess postharvest fruit decay incidence across the following three production systems: conventional, integrated pest management (IPM), and reduced IPM, for the cultivar 'Érdi bőtermő'. The results showed that modified atmosphere packaging effectively preserved or slightly increased fruit firmness (ranging from 27.1 to 46%) compared to control fruits across all cultivars. Fruit weight loss (ranging from 1.18 to 26.1%) was also significantly reduced under modified atmosphere packaging compared to normal atmospheric storage. Over a 14-day period, the decay incidence of harvested fruits consistently increased, with major losses from day 6 onward (ranging from 17.7 to 77.0% at shelf-life day 14) across all cultivars and years. After six weeks of normal atmospheric cold storage, decay incidence remained low (ranging from 0 to 9.4%) and was comparable across all cultivars. Both normal atmospheric and modified atmosphere packaging storage maintained sour cherry quality during cold storage, but the shelf-life of the fruits was significantly shorter at room temperature, regardless of the storage methods. Fruit decay incidence at harvest was highest in the reduced IPM system (ranging from 3.2 to 6.4%), significantly exceeding those observed in the conventional and IPM systems. In post-MAP cold storage, decay incidence followed the following order: conventional < IPM < reduced IPM, with significant differences observed only between the conventional and reduced IPM systems. In conclusion, our results suggest that sour cherries produced under conventional or IPM systems and stored in modified atmosphere packaging offer an effective postharvest strategy for preserving fruit quality. [ABSTRACT FROM AUTHOR]

A library of C-3 functionalized flavones was successfully provided via palladium-catalyzed amino- and aryloxycarbonylation reactions of 3-iodoflavone (1), under mild conditions. This methodology showed good functional group tolerance using a variety of amines and phenols, under an atmospheric pressure of carbon monoxide as a carbonyl source. While the flavone-3-carboxamides (2a-t) were produced in 22–79%, the flavone-3-carboxylates (4a′-l′) were obtained in excellent yields (up to 88%), under identical reaction conditions, just by switching N-nucleophiles to O-nucleophiles. The convenient availability of the involved starting materials confers simplicity to this approach to design new C-3-substituted flavones of biological relevance. The solid-state structures of flavone-3-carboxamide (2r) and flavone-3-ester (4f′) were further studied by single-crystal XRD analysis. [ABSTRACT FROM AUTHOR]

In 2019, random samples of Panicum miliaceum growing as a weed were surveyed to uncover their virus infections at two locations in Hungary. This pilot study revealed infection with three viruses, two appearing for the first time in the country. As follow-up research, in the summer of 2021, we collected symptomatic leaves of several monocotyledonous plants in the same locations and determined their viromes using small RNA high-throughput sequencing (HTS). As a result, we have identified the presence of wheat streak mosaic virus (WSMV), barley yellow striate mosaic virus (BYSMV), barley virus G (BVG), and two additional viruses, namely Aphis glycines virus 1 (ApGlV1) and Ljubljana dicistrovirus 1 (LDV1), which are described for the first time in Hungary. New hosts of the viruses were identified: Cynodon dactylon is a new host of BYSMV and LDV1, Echinocloa crus-galli is a new host of BVG, ApGlV1 and LDV1, Sorghum halepense is a new host of ApGlV1, and Panicum miliaceum is a new host of LDV1. At the same time, Zea mays is a new host of ApGlV1 and LDV1. Small RNA HTS diagnosed acute infections but failed to detect persistent ones, which could be revealed using RT-PCR. The infection rates at the different locations and plant species were different. The phylogenetic analyses of the sequenced virus variants suggest that the tested monocotyledonous weeds can host different viruses and play a virus reservoir role. Viral spread from the reservoir species relies on the activity of insect vectors, which is why their management requires an active role in plant protection strategies, which need careful planning in the changing environment. [ABSTRACT FROM AUTHOR]

Intestinal digestion and absorption are complex processes; thus, it is a challenge to imitate them realistically. There are numerous approaches available, with different disadvantages and advantages. The simplest methods to mimic absorption are the non-cell-based transport models but these lack important characteristics of enterocytes of the intestine. Therefore, the most often used method is to measure absorption through viable mammalian cells (most commonly Caco-2 cells, cultured on membrane insert plates), which not only assures the incorporation of brush border enzymes (responsible for the final digestion of peptides and disaccharides), it also simulates the absorption process. This means that influx/efflux transporter-facilitated transport, carrier-mediated transport, endocytosis, and transcytosis is also imitated besides passive diffusion. Still, these also lack the complexity of intestinal epithelium. Organoids or ex vivo models are a better approach if we want to attain precision but the highest accuracy can be achieved with microfluidic systems (gut-on-a-chip models). We propose that more research is necessary, and food absorption should also be studied on gut-on-a-chips, especially with fragmented organoids. Our review supports the choices of a proper intestinal epithelium model, which may have a key role in functional food development, nutrition studies, and toxicity assessment. [ABSTRACT FROM AUTHOR]

The aim of this study is to investigate consumer attitudes toward the quality and availability of gluten-free products. For this purpose, quantitative research was conducted using a questionnaire completed by 107 respondents who consumed gluten-free products. The results of the research showed that most consumers prepared their own meals, paid attention to ingredients, and strictly adhered to a gluten-free diet. About 10% of the respondents declared that they occasionally did not follow a gluten-free diet. Respondents were generally not satisfied with the price and availability of gluten-free products on the market. An additional aggravating circumstance for them when eating out was that restaurants generally do not have a sufficient selection of gluten-free dishes in their daily offer. Although most of them stated that they buy basic, well-known gluten-free products, they want to try new products with different, new flavors. This demand offers food manufacturers the opportunity to capitalize on the rapidly growing gluten-free market by developing creative and sustainable products that not only meet dietary requirements but also exceed consumer expectations. By focusing on innovation and variety, companies can participate in a profitable market while contributing to the growth of a more sustainable economy. The results of this study pointed to consumer preferences that could be used to influence the improvement and development of new gluten-free products, which are on the rise and will have a better nutritional quality and thus had a positive impact on health. [ABSTRACT FROM AUTHOR]

Inadequate adherence to a gluten-free diet in coeliac disease triggers autoimmune reactions and can reduce the quality of life. The strict diet requires constant vigilance, which can cause psychological distress. Our research aimed to assess the quality of life in adult patients with coeliac disease and to find a correlation between quality of life, dietary intervention, and adherence. The study included 51 adult patients with coeliac disease who completed a quality-of-life questionnaire. Adherence was assessed using serological tests and a dietary adherence test. The patients were divided into two groups: those on a gluten-free diet for at least three months (Group I) and newly diagnosed patients (Group II). Group I showed a significant decrease in the dysphoria subscale of the quality-of-life test between the first and last surveys. Poor quality of life was associated with worse adherence in Group II. A higher "Health concerns" quality of life subscale score was also associated with worse adherence in Group II. Our results suggest that dietetic care may be beneficial for patients with coeliac disease by reducing dysphoria. We recommend regular and long-term dietary monitoring from diagnosis to ensure adherence to a gluten-free diet and to maintain quality of life. [ABSTRACT FROM AUTHOR]

With the global population projected to reach 8.6 billion by 2050 and urbanization on the rise, sustainable food production in cities becomes imperative. Vertical farming presents a promising solution to meet this challenge by utilizing space-efficient, controlled-environment agriculture techniques. In a vertical farming system, high quality, high nutritional value products can be produced with minimum water consumption, using LEDs as energy-efficient light sources. Microgreens are a new market category of vegetables among sprouts and baby leaf greens. The most critical challenge in their cultivation is the choice of growing medium, lighting, and light spectrum, which affect photosynthesis, plant growth, and yield. This review explores various cultivation methods, including hydroponics, within the context of vertical farming. Using current research, it investigates the effect of LED lighting on the physiological properties and growth of microgreens and baby leaf lettuce, but further research is needed to determine the response of the varieties and the optimal light spectrum ratios to meet their needs. [ABSTRACT FROM AUTHOR]

Alterations to intestinal microbiota are assumed to occur in the pathogenesis of inflammatory bowel disease (IBD). This study aims to analyze the association of fecal microbiota composition, body composition, and lipid characteristics in patients with Crohn's disease (CD). In our cross-sectional study, patients with CD were enrolled and blood and fecal samples were collected. Clinical and endoscopic disease activity and body composition were assessed and laboratory tests were made. Fecal bacterial composition was analyzed using the shotgun method. Microbiota alterations based on obesity, lipid parameters, and disease characteristics were analyzed. In this study, 27 patients with CD were analyzed, of which 37.0% were obese based on visceral fat area (VFA). Beta diversities were higher in non-obese patients (p < 0.001), but relative abundances did not differ. C. innocuum had a higher abundance at a high cholesterol level than Bacillota (p = 0.001, p = 0.0034). Adlercreutzia, B. longum, and Blautia alterations were correlated with triglyceride levels. Higher Clostridia (p = 0.009) and B. schinkii (p = 0.032) and lower Lactobacillus (p = 0.035) were connected to high VFA. Disease activity was coupled with dysbiotic elements. Microbiota alterations in obesity highlight the importance of gut microbiota in diseases with a similar inflammatory background and project therapeutic options. [ABSTRACT FROM AUTHOR]

This study focuses on polyamide 6/organo-modified montmorillonite (PA6/OMMT) nanocomposites as potential liner materials, given the growing interest in enhancing the performance of type IV composite overwrapped hydrogen storage pressure vessels. The mechanical properties of PA6/OMMT composites with varying filler concentrations were investigated across a temperature range relevant to hydrogen storage conditions (−40 °C to +85 °C). Liner collapse, a critical issue caused by rapid gas discharge, was analyzed using an Ishikawa diagram to identify external and internal factors. Mechanical testing revealed that higher OMMT content generally increased stiffness, especially at elevated temperatures. The Young's modulus and first yield strength exhibited non-linear temperature dependencies, with 1 wt. per cent OMMT content enhancing yield strength at all tested temperatures. Dynamic mechanical analysis (DMA) indicated that OMMT improves the storage modulus, suggesting effective filler dispersion, but it also reduces the toughness and heat resistance, as evidenced by lower glass transition temperatures. This study underscores the importance of optimizing OMMT content to balance mechanical performance and thermal stability for the practical application of PA6/OMMT nanocomposites in hydrogen storage pressure vessels. [ABSTRACT FROM AUTHOR]

Retinal ischemia–reperfusion (I/R) injury is a critical pathogenic mechanism in various eye diseases, and an effective therapeutic strategy remains unresolved. Natural derivatives have recently reemerged; therefore, in our present study, we examined the potential therapeutic effects of a stilbenoid that is chemically related to resveratrol. Pterostilbene, recognized for its anti-inflammatory, anti-carcinogenic, anti-diabetic, and neuroprotective properties, counteracts oxidative stress during I/R injury through various mechanisms. This study explored pterostilbene as a retinoprotective agent. Male Sprague Dawley rats underwent retinal I/R injury and one-week reperfusion and were treated with either vehicle or pterostilbene. After this functional electroretinographical (ERG) measurement, Western blot and histological analyses were performed. Pterostilbene treatment significantly improved retinal function, as evidenced by increased b-wave amplitude on ERG. Histological studies showed reduced retinal thinning and preserved the retinal structure in the pterostilbene-treated groups. Moreover, Western blot analysis revealed a decreased expression of glial fibrillary acidic protein (GFAP) and heat shock protein 70 (HSP70), indicating reduced glial activation and cellular stress. Additionally, the expression of pro-apoptotic and inflammatory markers, poly(ADP-ribose) polymerase 1 (PARP1) and nuclear factor kappa B (NFκB) was significantly reduced in the pterostilbene-treated group. These findings suggest that pterostilbene offers protective effects on the retina by diminishing oxidative stress, inflammation, and apoptosis, thus preserving retinal function and structure following I/R injury. This study underscores pterostilbene's potential as a neuroprotective therapeutic agent for treating retinal ischemic injury and related disorders. [ABSTRACT FROM AUTHOR]

This study explores the efficacy of bio-efficient solutions, specifically plant growth-promoting microorganisms (PGPMs), in sustainable soil management. This research was conducted in 2020. It evaluates the impact of various single microbial inoculants, including Enterobacter ludwigii, Bacillus subtilis, Pseudomonas fluorescens, Kosakonia cowanii, and Trichoderma harzianum, on plant growth soil enzyme activity and organism abundance. Perennial ryegrass and mustard were used as test plants, in controlled environmental conditions. The results show generally positive effects of microbial inoculants on plant biomass (E. ludwigii increased ryegrass biomass by 9.75%, and P. fluorescens increased mustard biomass by up to 38.81% compared to the control) and on soil microbial activities. Our study further investigated the combined application of all these strains in five different soil types and textures. The results highlight the significance of soil physicochemical properties in determining inoculant efficacy; we found that clayey soils with higher colloid content support more robust microbial activity. Additionally, using natural clay minerals like alginite for enhancing soil conditions showed promising interactions with microbial inoculants, although application requires further optimization. These findings suggest that integrating microbial inoculants in sustainable agricultural practices could enhance plant growth, improve soil health, and reduce the need of chemical fertilizers. Future research should aim to refine the combinations and application methods of these bio-efficient solutions for broader agricultural applicability. [ABSTRACT FROM AUTHOR]

The incidence of diabetes, including type 2 diabetes (T2DM), is increasing sharply worldwide. To reverse this, more effective approaches in prevention and treatment are needed. In our review, we sought to summarize normal insulin action and the pathways that primarily influence the development of T2DM. Normal insulin action involves mitogenic and metabolic pathways, as both are important in normal metabolic processes, regeneration, etc. However, through excess energy, both can be hyperactive or attenuated/inactive leading to disturbances in the cellular and systemic regulation with the consequence of cellular stress and systemic inflammation. In this review, we detailed the beneficial molecular changes caused by some important components of nutrition and by exercise, which act in the same molecular targets as the developed drugs, and can revert the damaged pathways. Moreover, these induce entire networks of regulatory mechanisms and proteins to restore unbalanced homeostasis, proving their effectiveness as preventive and complementary therapies. These are the main steps for success in prevention and treatment of developed diseases to rid the body of excess energy, both from stored fats and from overnutrition, while facilitating fat burning with adequate, regular exercise in healthy people, and together with necessary drug treatment as required in patients with insulin resistance and T2DM. [ABSTRACT FROM AUTHOR]

The behavior and presence of actin-regulating proteins are characteristic of various clinical diseases. Changes in these proteins significantly impact the cytoskeletal and regenerative processes underlying pathological changes. Pituitary adenylate cyclase-activating polypeptide (PACAP), a cytoprotective neuropeptide abundant in the nervous system and endocrine organs, plays a key role in neuron differentiation and migration by influencing actin. This study aims to elucidate the role of PACAP as an actin-regulating polypeptide, its effect on actin filament formation, and the underlying regulatory mechanisms. We examined PACAP27, PACAP38, and PACAP6-38, measuring their binding to actin monomers via fluorescence spectroscopy and steady-state anisotropy. Functional polymerization tests were used to track changes in fluorescent intensity over time. Unlike PACAP27, PACAP38 and PACAP6-38 significantly reduced the fluorescence emission of Alexa488-labeled actin monomers and increased their anisotropy, showing nearly identical dissociation equilibrium constants. PACAP27 showed weak binding to globular actin (G-actin), while PACAP38 and PACAP6-38 exhibited robust interactions. PACAP27 did not affect actin polymerization, but PACAP38 and PACAP6-38 accelerated actin incorporation kinetics. Fluorescence quenching experiments confirmed structural changes upon PACAP binding; however, all studied PACAP fragments exhibited the same effect. Our findings indicate that PACAP38 and PACAP6-38 strongly bind to G-actin and significantly influence actin polymerization. Further studies are needed to fully understand the biological significance of these interactions. [ABSTRACT FROM AUTHOR]

Drone-based imaging polarimetry is a valuable new tool for the remote sensing of the polarization characteristics of the Earth's surface. After briefly reviewing two earlier drone-polarimetric studies, we present here the results of our drone-polarimetric campaigns, in which we measured the reflection–polarization patterns of greenhouses. From the measured patterns of the degree and angle of linear polarization of reflected light, we calculated the measure (plp) of polarized light pollution of glass surfaces. The knowledge of polarized light pollution is important for aquatic insect ecology, since polarotactic aquatic insects are the endangered victims of artificial horizontally polarized light sources. We found that the so-called Palm House of a botanical garden has only a low polarized light pollution, 3.6% ≤ plp ≤ 13.7%, while the greenhouses with tilted roofs are strongly polarized-light-polluting, with 24.8% ≤ plp ≤ 40.4%. Similarly, other tilted-roofed greenhouses contain very high polarized light pollution, plp ≤ 76.7%. Under overcast skies, the polarization patterns and plp values of greenhouses practically only depend on the direction of view relative to the glass surfaces, as the rotationally invariant diffuse cloud light is the only light source. However, under cloudless skies, the polarization patterns of glass surfaces significantly depend on the azimuth direction of view and its angle relative to the solar meridian because, in this case, sunlight is the dominant light source, rather than the sky. In the case of a given direction of view, those glass surfaces are the strongest polarized-light-polluting, from which sunlight and/or skylight is reflected at or near Brewster's angle in a nearly vertical plane, i.e., with directions of polarization close to horizontal. Therefore, the plp value is usually greatest when the sun shines directly or from behind. The plp value of greenhouses is always the smallest in the green spectral range due to the green plants under the glass. [ABSTRACT FROM AUTHOR]

Landraces are considered a crucial component of biodiversity conservation, serving as a reservoir of genetic diversity. Consequently, the collection, cultivation, and detailed characterization of such landraces constitute an inherent aspect of the world's natural resource heritage. This effort holds promise for the development of elite varieties capable of thriving amidst continuous global climate fluctuations. In this context, we conducted a comprehensive assessment of the main agronomic attributes, physico-chemical properties, and functional quality traits of the major hot pepper landraces adapted to diverse climatic conditions in Tunisia. These landraces include 'Dhirat', 'Semmane', 'Beldi', 'Nabeul', 'Jerid', 'Mahdia', 'Cayenne', 'Kairouan', and 'Baklouti'. Most of the pepper landraces exhibited satisfactory yields, ranging from 1163.25 to 1841.67 g plant−1 in 'Jerid' and 'Kairouan', respectively, indicating robust productivity, especially under prevailing climatic changes and high temperatures during both growing cycles. The levels of antioxidants comprising capsaicinoids, carotenoids, phenolics, and tocopherols, as well as radical scavenging activity, emerged as key discriminating factors among pungent pepper landraces. Irrespective of genotype, capsaicin and dihydrocapsaicin constituted the major capsaicinoids, accounting for 44–91% of the total capsaicinoids content. Total capsaicinoids ranged from 1.81 µg g−1 fw to 193.71 µg g−1 fw, with 'Baklouti' and 'Jerid' identified as the most pungent landraces. Total carotenoids ranged from 45.94 µg g−1 fw to 174.52 µg g−1 fw, with 'Semmane' and 'Jerid' exhibiting the highest levels. Considerable variation was observed in β-carotene content, spanning from 3% to 24% of the total carotenoids. α-Tocopherol content ranged from 19.03 µg g−1 fw in 'Kairouan' to 30.93 µg g−1 fw in 'Beldi', exerting a notable influence on the overall tocopherol content. Conversely, the β- and γ-tocopherol isomers were detected at very low concentrations. The total vitamin C content ranged from 132 mg 100g−1 fw in 'Mahdia' to 200 mg 100 g−1 fw in 'Nabeul', indicating relatively low genetic variability. However, large variability was detected in total phenolics content, ranging from 168.58 mg GAE kg−1 fw in 'Beldi' to 302.98 mg GAE kg−1 fw in 'Cayenne'. Landraces such as 'Dhirat', 'Nabeul', 'Semmane', 'Kairouan', 'Cayenne', and 'Mahdia' appear suitable for both fresh consumption and processing, owing to their favorable average fruit weight, soluble solids content, and bioactive content. Among the pepper landraces tested, 'Cayenne' achieved the highest value of radical scavenging activity in both hydrophilic and lipophilic fractions (RSAHF and RSALF), with variations ranging from 59% to 120% for RSAHF and from 4% to 63% for RSALF. This study aims to preserve and enhance the value of local genetic resources and contribute to identify desirable traits for incorporation into breeding programs to develop high-quality, high-yielding landraces and elite lines. [ABSTRACT FROM AUTHOR]

Lymphocytic choriomeningitis virus (LCMV) is a neglected rodent-borne arenavirus, primarily spread by common house mouse species. Acquired human infections range from asymptomatic to mild flu-like symptoms and self-resolving neurological diseases. In contrast, intrauterine LCMV infection is associated with high mortality and morbidity. Infection of the fetus often leads to fetal death, and surviving fetuses may develop vision impairment and central nervous system developmental disorders. LCMV is mainly diagnosed by serological methods using in-house indirect immunofluorescence assays. LCMV nucleic acid is detected by the nested RT-PCR method and confirmed by Sanger sequencing. In Hungary, 23 acquired lymphocytic choriomeningitis cases were diagnosed between 2017 and 2023. Ten out of 23 confirmed patients proved to be positive by the PCR method. Two cases of intrauterine LCMV infections were detected in 2019 and 2021, respectively. The IgG antibody titers measured in the infant's serum samples were much higher than the IgG titers of the maternal serum samples. Both IgM and IgA antibodies were detectable in the infants' sera. As the microbiological diagnosis of LCMV is rather challenging and the symptoms are very similar to the clinical picture of other common teratogenic pathogens such as cytomegalovirus or Toxoplasma gondii, intrauterine LCMV infections might still be underdiagnosed. [ABSTRACT FROM AUTHOR]

Robot-Assisted Minimally Invasive Surgery (RAMIS) marks a paradigm shift in surgical procedures, enhancing precision and ergonomics. Concurrently it introduces complex stress dynamics and ergonomic challenges regarding the human–robot interface and interaction. This study explores the stress-related aspects of RAMIS, using the da Vinci XI Surgical System and the Sea Spikes model as a standard skill training phantom to establish a link between technological advancement and human factors in RAMIS environments. By employing different physiological and kinematic sensors for heart rate variability, hand movement tracking, and posture analysis, this research aims to develop a framework for quantifying the stress and ergonomic loads applied to surgeons. Preliminary findings reveal significant correlations between stress levels and several of the skill-related metrics measured by external sensors or the SURG-TLX questionnaire. Furthermore, early analysis of this preliminary dataset suggests the potential benefits of applying machine learning for surgeon skill classification and stress analysis. This paper presents the initial findings, identified correlations, and the lessons learned from the clinical setup, aiming to lay down the cornerstones for wider studies in the fields of clinical situation awareness and attention computing. [ABSTRACT FROM AUTHOR]

The accelerated expansion of the Universe is impressively well described by a cosmological constant. However, the observed value of the cosmological constant is much smaller than expected based on quantum field theories. Recent efforts to achieve consistency in these theories have proposed a relationship between Dark Energy and the most compact objects, such as black holes (BHs). However, experimental tests are very challenging to devise and perform. In this article, we present a testable model with no cosmological constant in which the accelerated expansion can be driven by black holes. The model couples the expansion of the Universe (the Friedmann equation) with the mass function of cosmological halos (using the Press–Schechter formalism). Through the observed link between halo masses and BH masses, one thus gets a coupling between the expansion rate of the Universe and the BHs. We compare the predictions of this simple BH model with SN1a data and find poor agreement with observations. Our method is sufficiently general to allow us to also test a fundamentally different model, also without a cosmological constant, where the accelerated expansion is driven by a new force proportional to the internal velocity dispersion of galaxies. Surprisingly enough, this model cannot be excluded using the SN1a data. [ABSTRACT FROM AUTHOR]

Mining and metallurgy are the main sources of soil contamination with harmful metals, posing a significant threat to human health and ecosystems. River floodplains in the vicinity of metal mines or industrial plants are often subject to flooding with sediments containing heavy metals, which can be harmful to the soil ecosystem. This study aimed to investigate the microbial properties of the soil at a metal-contaminated site and to determine the significant relationships between the biological and chemical properties of the soil. The study site was located near the village of Gyöngyösoroszi, in the Mátra mountain region of Northwest Hungary. A phytoremediation experiment was conducted in a metal-polluted floodplain using willow and corn plantations. The soil basal respiration, substrate-induced respiration, soil microbial biomass carbon (MBC), acid phosphatase activities, and soil chemical properties were measured. The soil of the contaminated sites had significantly higher levels of As, Pb, Zn, Cu, Cd, and Ca, whereas the unpolluted sites had significantly higher levels of phosphorus and potassium. The substrate-induced respiration showed a positive correlation with MBC and negative correlations with the metabolic quotient (qCO2). The soil plasticity index and phosphorus showed a positive correlation with MBC, whereas salinity and the presence of Cd, Pb, Zn, As, and Cu showed a negative correlation. Acid phosphomonoesterase activity negatively correlated with the plant-available phosphorus content and MBC, but was positively correlated with the contents of toxic elements, including cadmium, lead, zinc, arsenic, and copper. This study found a significant correlation between the qCO2 and the toxic element content. This suggests that an enhanced metabolic quotient (qCO2), together with a decreased MBC/SOC ratio, could be used to indicate the harmful effect of soil contamination by heavy metals in floodplain soils. [ABSTRACT FROM AUTHOR]

Background: The field of smart devices and physical activity is evolving rapidly, with a wide range of devices measuring a wide range of parameters. Scientific articles look at very different populations in terms of the impact of smart devices but do not take into account which characteristics of the devices are important for the group and which may influence the effectiveness of the device. In our study, we aimed to analyse articles about the impact of smart devices on physical activity and identify the characteristics of different target groups. Methods: Queries were run on two major databases (PubMed and Web of Science) between 2017 and 2024. Duplicates were filtered out, and according to a few main criteria, inappropriate studies were excluded so that 37 relevant articles were included in a more detailed analysis. Results: Four main target groups were identified: healthy individuals, people with chronic diseases, elderly people, and competitive athletes. We identified the essential attributes of smart devices by target groups. For the elderly, an easy-to-use application is needed. In the case of women, children, and elderly people, gamification can be used well, but for athletes, specific measurement tools and accuracy may have paramount importance. For most groups, regular text messages or notifications are important. Conclusions: The use of smart devices can have a positive impact on physical activity, but the context and target group must be taken into account to achieve effectiveness. [ABSTRACT FROM AUTHOR]

Specific polarized light pollution (PLP) means the adverse influences of strongly and horizontally polarized light reflected from smooth and dark artificial surfaces on polarotactic water-seeking aquatic insects. Typical PLP sources are photovoltaic panels. Using drone-based imaging polarimetry, in a solar panel farm, we measured the reflection-polarization patterns of fixed-tilt photovoltaic panels from the viewpoint of flying polarotactic aquatic insects, which are the most endangered targets and potential victims of such panels. We found that the temporal changes in PLP were complementary for the two orthogonal viewing directions relative to the panel rows. The estimated magnitude plp of the polarized light pollution of solar panels viewed parallel to the panel rows was the highest (primary peak plp = 49–58% after sunrise and secondary peak plp = 35–48% prior to sunset) at low solar elevations, after sunrise and at or prior to sunset, when many aquatic insect species fly and seek water bodies. On the other hand, the PLP of solar panels viewed perpendicular to the panel rows was the highest (plp = 29–35%) at the largest solar elevations, near noon, when numerous flying aquatic insect species also seek water. Solar panel farms near wetlands can, therefore, be dangerous for these insects. [ABSTRACT FROM AUTHOR]

The occurrence of the market-leading glyphosate active ingredient in surface waters is a globally observed phenomenon. Although co-formulants in pesticide formulations were considered inactive components from the aspects of the required main biological effect of the pesticide, several studies have proven the high individual toxicity of formulating agents, as well as the enhanced combined toxicity of the active ingredients and other components. Since the majority of active ingredients are present in the form of chemical mixtures in our environment, the possible combined toxicity between active ingredients and co-formulants is particularly important. To assess the individual and combined phytotoxicity of the components, glyphosate was tested in the form of pure active ingredient (glyphosate isopropylammonium salt) and herbicide formulations (Roundup Classic and Medallon Premium) formulated with a mixture of polyethoxylated tallow amines (POEA) or alkyl polyglucosides (APG), respectively. The order of acute toxicity was as follows for Roundup Classic: glyphosate < herbicide formulation < POEA. However, the following order was demonstrated for Medallon Premium: herbicide formulation < glyphosate < APG. Increased photosynthetic activity was detected after the exposure to the formulation (1.5–5.8 mg glyphosate/L and 0.5–2.2 mg POEA/L) and its components individually (glyphosate: 13–27.2 mg/L, POEA: 0.6–4.8 mg/L), which indicates hormetic effects. However, decreased photosynthetic activity was detected at higher concentrations of POEA (19.2 mg/L) and Roundup Classic (11.6–50.6 mg glyphosate/L). Differences were demonstrated in the sensitivity of the selected algae species and, in addition to the individual and combined toxicity of the components presented in the glyphosate-based herbicides. Both of the observed inhibitory and stimulating effects can adversely affect the aquatic ecosystems and water quality of surface waters. [ABSTRACT FROM AUTHOR]

The environmental load by isoxaflutole and its formulated herbicide products has increasingly become apparent because, after the ban of atrazine, isoxaflutole has become its replacement active ingredient (a.i.). Obtaining information regarding the fate of this a.i. in environmental matrices and its ecotoxicological effects on aquatic organisms is essential for the risk assessment of the herbicide. In this study, the effects of Merlin Flexx- and Merlin WG75 formulated isoxaflutole-based herbicide products and two selected additives (cyprosulfamide safener and 1,2-benzisothiazol-3(2H)-one antimicrobial agent) were investigated on Raphidocelis subcapitata in growth inhibition assays. In ecotoxicological tests, two conventional (optical density and chlorophyll-a content) and two induced fluorescence-based (Fv*/Fp: efficiency of the photosystem PSII and Rfd* changes in the observed ratio of fluorescence decrease) endpoints were determined by UV-spectrophotometer and by our FluoroMeter Module, respectively. Furthermore, dissipation of isoxaflutole alone and in its formulated products was examined by an HPLC-UV method. In ecotoxicological assays, the fluorescence-based Rfd* was observed as the most sensitive endpoint. In this study, the effects of the safener cyprosulfamide and the antimicrobial agent 1,2-benzisothiazol-3(2H)-one on R. subcapitata is firstly reported. The results indicated that the isoxaflutole-equivalent toxicity of the mixture of the isoxaflutole–safener–antimicrobial agent triggered lower toxicity (EC50 = 2.81 ± 0.22 mg/L) compared to the individual effect of the a.i. (EC50 = 0.02 ± 0.00 mg/L). The Merlin Flexx formulation (EC50 = 27.04 ± 1.41 mg/L) was found to be approximately 50-fold less toxic than Merlin WG75, which can be explained by the different chemical characteristics and quantity of additives in them. The additives influenced the dissipation of the a.i. in Z8 medium, as the DT50 value decreased by approximately 1.2- and 3.5-fold under light and dark conditions, respectively. [ABSTRACT FROM AUTHOR]

Among patients on peritoneal dialysis (PD), 50–80% will develop peritoneal fibrosis, and 0.5–4.4% will develop life-threatening encapsulating peritoneal sclerosis (EPS). Here, we investigated the role of extracellular vesicles (EVs) on the TGF-β- and PDGF-B-driven processes of peritoneal fibrosis. EVs were isolated from the peritoneal dialysis effluent (PDE) of children receiving continuous ambulatory PD. The impact of PDE-EVs on the epithelial–mesenchymal transition (EMT) and collagen production of the peritoneal mesothelial cells and fibroblasts were investigated in vitro and in vivo in the chlorhexidine digluconate (CG)-induced mice model of peritoneal fibrosis. PDE-EVs showed spherical morphology in the 100 nm size range, and their spectral features, CD63, and annexin positivity were characteristic of EVs. PDE-EVs penetrated into the peritoneal mesothelial cells and fibroblasts and reduced their PDE- or PDGF-B-induced proliferation. Furthermore, PDE-EVs inhibited the PDE- or TGF-β-induced EMT and collagen production of the investigated cell types. PDE-EVs contributed to the mesothelial layer integrity and decreased the submesothelial thickening of CG-treated mice. We demonstrated that PDE-EVs significantly inhibit the PDGF-B- or TGF-β-induced fibrotic processes in vitro and in vivo, suggesting that EVs may contribute to new therapeutic strategies to treat peritoneal fibrosis and other fibroproliferative diseases. [ABSTRACT FROM AUTHOR]

Hypoxia-inducible factor-1 (HIF-1) is a heterodimer transcription factor composed of an alpha and a beta subunit. HIF-1α is a master regulator of cellular response to hypoxia by activating the transcription of genes that facilitate metabolic adaptation to hypoxia. Since chondrocytes in mature articular cartilage reside in a hypoxic environment, HIF-1α plays an important role in chondrogenesis and in the physiological lifecycle of articular cartilage. Accumulating evidence suggests interactions between the HIF pathways and the circadian clock. The circadian clock is an emerging regulator in both developing and mature chondrocytes. However, how circadian rhythm is established during the early steps of cartilage formation and through what signaling pathways it promotes the healthy chondrocyte phenotype is still not entirely known. This narrative review aims to deliver a concise analysis of the existing understanding of the dynamic interplay between HIF-1α and the molecular clock in chondrocytes, in states of both health and disease, while also incorporating creative interpretations. We explore diverse hypotheses regarding the intricate interactions among these pathways and propose relevant therapeutic strategies for cartilage disorders such as osteoarthritis. [ABSTRACT FROM AUTHOR]

Thin films of mixed MoO3 and WO3 were obtained using reactive magnetron sputtering onto ITO-covered glass, and the optimal composition was determined for the best electrochromic (EC) properties. A combinatorial material synthesis approach was applied throughout the deposition experiments, and the samples represented the full composition range of the binary MoO3/WO3 system. The electrochromic characteristics of the mixed oxide films were determined with simultaneous measurement of layer transmittance and applied electric current through the using organic propylene carbonate electrolyte cells in a conventional three-electrode configuration. Coloration efficiency data evaluated from the primary data plotted against the composition displayed a characteristic maximum at around 60% MoO3. Our combinatorial approach allows the localization of the maximum at 5% accuracy. [ABSTRACT FROM AUTHOR]

The tomato as a raw material for processing is globally important and is pivotal in dietary and agronomic research due to its nutritional, economic, and health significance. This study explored the potential of machine learning (ML) for predicting tomato quality, utilizing data from 48 cultivars and 28 locations in Hungary over 5 seasons. It focused on °Brix, lycopene content, and colour (a/b ratio) using extreme gradient boosting (XGBoost) and artificial neural network (ANN) models. The results revealed that XGBoost consistently outperformed ANN, achieving high accuracy in predicting °Brix (R² = 0.98, RMSE = 0.07) and lycopene content (R² = 0.87, RMSE = 0.61), and excelling in colour prediction (a/b ratio) with a R² of 0.93 and RMSE of 0.03. ANN lagged behind particularly in colour prediction, showing a negative R² value of −0.35. Shapley additive explanation's (SHAP) summary plot analysis indicated that both models are effective in predicting °Brix and lycopene content in tomatoes, highlighting different aspects of the data. SHAP analysis highlighted the models' efficiency (especially in °Brix and lycopene predictions) and underscored the significant influence of cultivar choice and environmental factors like climate and soil. These findings emphasize the importance of selecting and fine-tuning the appropriate ML model for enhancing precision agriculture, underlining XGBoost's superiority in handling complex agronomic data for quality assessment. [ABSTRACT FROM AUTHOR]

Retinal vascular diseases and consequential metabolic disturbances in the eye are major concerns for healthcare systems all around the world. BGP-15, a drug candidate small-molecule [O-(3-piperidino-2-hydroxy-1-propyl) nicotinic amidoxime dihydrochloride], has been formerly demonstrated by our workgroup to be retinoprotective both in the short and long term. Based on these results, the present study was performed to investigate the efficacy of BGP in an eyedrop formulation containing sulfobutylether-β-cyclodextrin (SBECD), which is a solubility enhancer as well. Electroretinographical evaluations were carried out and BGP was demonstrated to improve both scotopic and photopic retinal a- and b-waves, shorten their implicit times and restore oscillatory potentials after ischemia–reperfusion. It was also observed to counteract retinal thinning after ischemia–reperfusion in the eyes of Sprague Dawley rats. This small-molecule drug candidate is able to compensate for experimental global eye ischemia–reperfusion injury elicited by ligation of blood vessels in rats. We successfully demonstrated that BGP is able to exert its protective effects on the retina even if administered in the form of eyedrops. [ABSTRACT FROM AUTHOR]

This article presents an open-source device illustrating the well-known magnetic levitation experiment. The uniqueness of this particular device lies in its exceptionally small dimensions, affordability and availability, which makes it a perfect design for take-home experiments for education but it can also serve as a referential design for testing various control algorithms in research. In addition, this paper provides a comprehensive hardware design for reproducibility along with the detailed derivation of the mathematical model, system identification and validation. Moreover, the introduced hardware comes with an easy-to-use open-source application programming interface in C/C++ for the Arduino IDE, Simulink and CircuitPython. REXYGEN, another environment similar to Simulink, had also been used to demonstrate the capabilities of the MagnetoShield. [ABSTRACT FROM AUTHOR]

The long time (2 h) required for measurement, expensive chemicals (Ag2SO4), and toxic reagents (K2Cr2O7, HgSO4) limit the application of the standard method for measuring the oxygen equivalent of organic content in wastewater (chemical oxygen demand, COD). In recent years, the COD has increasingly been replaced by the total organic carbon (TOC) parameter. Since the limit values of the pollution levels are usually given in terms of the COD, efforts are being made to find the correlation between these parameters. Several papers have published correlation analyses of COD and TOC for industrial and municipal wastewater, but the relationship has not been discussed for individual chemicals. Here, this relationship was investigated using 70 contaminants (laboratory chemicals, pharmaceuticals, and pesticides). The calculated COD values, in most cases, agreed, within ~10%, with the experimental ones; for tetracyclines and some chloroaromatic molecules, the measured values were 20–50% lower than the calculated values. The COD/TOC ratios were between 2 and 3: for macrolides, they were ~3; for fluoroquinolones and tetracyclines, they were ~2. The molecular structure dependence of the ratio necessitates the establishing of the correlation on an individual basis. In advanced oxidation processes (AOPs), the ratio changes during degradation, limiting the application of TOC instead of COD. [ABSTRACT FROM AUTHOR]

Nanotechnology is playing a significant role in modern life with tremendous potential and promising results in almost every domain, especially the pharmaceutical one. The impressive performance of nanomaterials is shaping the future of science and revolutionizing the traditional concepts of industry and research. Titanate nanotubes (TNTs) are one of these novel entities that became an appropriate choice to apply in several platforms due to their remarkable properties such as preparation simplicity, high stability, good biocompatibility, affordability and low toxicity. Surface modification of these nanotubes is also promoting their superior characters and contributing more to the enhancement of their performance. In this research work, an attempt was made to functionalize the surface of titanate nanotubes with carboxylic groups to increase their surface reactivity and widen the possibility of bonding different molecules that could not be bonded directly. Three carboxylic acids were investigated (trichloroacetic acid, citric acid and acrylic acid), and the prepared composites were examined using FT-IR and Raman spectroscopy, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and dynamic light scattering (DLS). The toxicity of these functionalized TNTs was also investigated using adherent cancer cell lines and fibroblasts to determine their safety profile and to draw the basic lines for their intended future application. Based on the experimental results, acrylic acid could be the suitable choice for permanent surface modification with multiple carboxylic groups due to its possibility to be polymerized, thus presenting the opportunity to link additional molecules of interest such as polyethylene glycol (PEG) and/or other molecules at the same time. [ABSTRACT FROM AUTHOR]

Glyphosate is the most widely used active ingredient (AI) in glyphosate-based herbicides (GBHs) worldwide and is also known to affect a variety of soil organisms. However, we know little about how the effects of glyphosate AIs differ from those of GBHs that also contain so-called inert co-formulants. We conducted a greenhouse experiment using the model cover crop white mustard (Sinapis alba) to investigate the effects of three GBHs (Roundup PowerFlex, Roundup LB Plus, and Touchdown Quattro) and their respective glyphosate AIs (glyphosate potassium, isopropylamine, and diammonium salt) on epedaphic springtails (Sminthurinus niger; Collembola) activity in soils with low (3.0%) or high (4.1%) organic matter content (SOM). Springtail activity was assessed using pitfall traps. Most GBHs and AIs reduced springtail activity compared to mechanical removal of mustard in the short-term and even up to 5 months after application. GBHs and AIs differed considerably in their effects on springtail activity, and effects were modified by SOM content. Our results highlight the need to (i) distinguish between the effects of glyphosate AIs and commercial GBH formulations, (ii) disclose all ingredients of GBHs, as co-formulants also affect non-target organisms, and (iii) include soil properties in ecotoxicological risk assessments for soil organisms to better characterize the situation in the field. [ABSTRACT FROM AUTHOR]

Tomato landraces have progressively faded into obscurity, making way for new hybrids and elite tomato cultivars. This study presents a comprehensive evaluation of the agronomic attributes, physicochemical properties, and functional traits across seven cultivars, comprising two high-pigment varieties, 'HLT−F81' and 'HLT−F82', as well as five underutilized ancient-tomato genotypes considered as landraces. Most of the studied genotypes exhibited satisfactory horticultural and processing traits. The average fruit weight ranged from 73.3 g in 'Rimone' to 91.83 g in 'HLT−F81', while the soluble solids content ranged from 4.66 °Brix in 'Justar' to 6.08 °Brix in 'HLT−F81'. The functional quality and the content of most antioxidants, as well as the antioxidant activity in both hydrophilic and lipophilic fractions, proved to be the most discriminating parameters among the tomato genotypes. The content of β−carotene and lycopene spanned from 2.94 mg kg−1 fw in 'Rio Grande' to 13.94 mg kg−1 fw in 'HLT−F82' and 227.8 mg kg−1 fw in 'HLT−F81', yielding large variations compared to 'Rio Grande'. The total phenolic content ranged from 139.83 mg GAE kg−1 fw in 'Rimone' to 352.41 mg GAE kg−1 fw in 'HLT-F81', while the flavonoid content varied from 136.16 mg RE kg−1 fw in 'Justar' to 311.23 mg RE kg−1 fw in 'HLT-F82'. The presence of tocopherol isomers was genotype-dependent, with a higher content in lines carrying the high-pigment mutations. Among the tested tomato genotypes, the high-pigment tomato line 'HLT−F81' achieved the highest hydrophilic and lipophilic antioxidant activity values. This study primarily focused on the recovery and valorization of tomato genetic resources and landraces. It also aimed to identify desirable horticultural (yield, low-input, and low-water demand), processing (°Brix and titratable acidity), and quality (rich antioxidant berries) traits for introgression into new tomato cultivars better suited to the evolving climate conditions of the near future. [ABSTRACT FROM AUTHOR]

Colon cancer is a leading cause of death worldwide. Identification of new molecular factors governing the invasiveness of colon cancer holds promise in developing screening and targeted therapeutic methods. The Tyrosine Kinase Substrate with four SH3 domains (TKS4) and the CD2-associated protein (CD2AP) have previously been linked to dynamic actin assembly related processes and cancer cell migration, although their co-instructive role during tumor formation remained unknown. Therefore, this study was designed to investigate the TKS4-CD2AP interaction and study the interdependent effect of TKS4/CD2AP on oncogenic events. We identified CD2AP as a novel TKS4 interacting partner via co-immunoprecipitation-mass spectrometry methods. The interaction was validated via Western blot (WB), immunocytochemistry (ICC) and proximity ligation assay (PLA). The binding motif of CD2AP was explored via peptide microarray. To uncover the possible cooperative effects of TKS4 and CD2AP in cell movement and in epithelial-mesenchymal transition (EMT), we performed gene silencing and overexpressing experiments. Our results showed that TKS4 and CD2AP form a scaffolding protein complex and that they can regulate migration and EMT-related pathways in HCT116 colon cancer cells. This is the first study demonstrating the TKS4-CD2AP protein–protein interaction in vitro, their co-localization in intact cells, and their potential interdependent effects on partial-EMT in colon cancer. [ABSTRACT FROM AUTHOR]

Managing crop yields and optimizing water use is a global challenge, as fresh water supply decreases rapidly and demand remains high. Therefore, understanding how plants react to varying water levels is crucial for efficient water usage. This study evaluates how tomato plants adapt to varying water levels (100%, 50% of crop evapotranspiration, and non-irrigated control) over two growing seasons in 2020 and 2021. Root images were captured weekly during an 8-week monitoring period in 2020 and 6 weeks in 2021 using a non-destructive CI-600 in-situ root imager at depths between 10 and 70 cm. Under water stress, plants developed deeper, more extensive root systems to maximize water uptake, consistent with prior research. Root depth and architecture varied with soil depth and the severity of water stress. Year-to-year variations were also found, likely due to changes in irrigation levels and environmental conditions such as temperature. SPAD values were higher under control conditions, especially in the 2021 growing season, suggesting reduced chlorophyll degradation, while no significant differences were observed in chlorophyll fluorescence (Fv/Fm) between treatments, suggesting stable photosynthetic efficiency under varied water stress conditions. These findings contribute to our understanding of root zone optimization and drought-resilient cultivar breeding, contributing to more sustainable agricultural practices. [ABSTRACT FROM AUTHOR]

Climate change and increasing human impacts are more emphasised in recharge regions, where the main flow direction is downward, resulting in negative water balance. Two wetlands located in the recharge position of regional groundwater flow systems were investigated in the Nyírség region, Hungary, as pilot areas for representing wetlands in similar hydraulic positions. Hydraulic data processes, chemical data evaluations, and numerical simulations revealed that the wetlands are fed via local flow systems, superimposing regional-scale recharge conditions in the area. The wetlands are discharge and flow-through types in connection with local flow systems. Nevertheless, in the case of significant regional water table decline—due to the high vulnerability of recharge areas to climate change—local flows are degraded, so they are not able to sustain the wetlands. To preserve the groundwater-dependent ecosystems in the areas, water retention at the local recharge areas of the wetlands may help in the mitigation of water level decline under present-day conditions. If the regional water table continues to decline, comprehensive water retention solutions are needed in the whole region. The results highlight that understanding the natural wetland–groundwater interactions at different scales is crucial for the preservation of wetlands and for successful water retention planning. [ABSTRACT FROM AUTHOR]

Glyphosate is the most widely used active ingredient (AI) in thousands of glyphosate-based herbicides (GBHs) worldwide. Short-term impacts of AIs or GBHs on earthworms are well known, but few studies have examined long-term legacy effects >3 months after application. In a pot experiment, we studied both short-term and long-term effects on deep burrowing earthworms (Lumbricus terrestris) and soil functions. Therefore, the cover crop Sinapis alba was grown in soils with either 3.0% or 4.1% soil organic matter content (SOM) and either sprayed with a GBH (Touchdown Quattro, Roundup PowerFlex, or Roundup LB Plus) or the respective glyphosate AI (diammonium-, potassium-, or isopropylamine-salt) or hand weeded (control). Long-term effects showed increased earthworm activity under GBHs even 4 months after application, but similar activity under AIs and control. Another application of the same treatments 5 months after the previous one also increased earthworm activity under GBHs, especially at high SOM levels. Water infiltration after a simulated heavy rainfall was 50% lower, and leaching was 30% higher under GBH than under AI application or hand weeding. Individual GBHs and AIs varied in their effects and were influenced by SOM and soil moisture. Full disclosure of all ingredients in GBH formulations would be necessary to allow a comprehensive assessment of environmental risks. [ABSTRACT FROM AUTHOR]

Mental and psychological disorders are serious health problems worldwide. Anxiety among high school students can affect school performance, relationships, and family life. Objective: Our aim is to understand the anxiety levels and associated factors among high school students and compare the results of psychological tests measuring anxiety with the cortisol levels obtained from biological sampling. Method: In our longitudinal follow-up study, we involved 125 individuals in May 2019. Validated measurement tools were used during questionnaire data collection, including the State–Trait Anxiety Inventory, Clear Communication Scale, Multiple Social Perceived Support Scale, and related HBSC questions. As objective data, we collected hair samples for cortisol level measurement. Results: At the end of the school year, the anxiety levels measured by psychological tests were significantly higher (p = 0.001) compared to the anxiety levels at the beginning of the next school year. Anxiety levels were higher among girls and were influenced by the type of school and parental expectations. Both state anxiety and trait anxiety showed a strong correlation with psychosomatic symptoms (p < 0.001) and anxiety arising from school expectations (p < 0.05). The changes in cortisol levels did not follow the changes in psychological tests. Cortisol level increased (p = 0.01) in the second sample. [ABSTRACT FROM AUTHOR]

Salt meadows, protected within National Parks, cannot be directly surveyed, yet understanding their soil condition is crucial. Our study indirectly estimates soil parameters (Total Salt Content (TSC), Na, and pH) related to salinization/sodification/alkalinization using spectral indices and UAV survey-derived elevation model, focusing on continental lowland salt meadows. A vegetation map was created using 16 spectral indices and a Digital Elevation Model calculated from RGB orthophotos using photogrammetry. Field observations helped define habitat types based on the General National Habitat Classification System (Hungary), and quadrats with complete coverage of specific plant species were identified. Machine learning was employed on 84 training quadrats to develop a prediction algorithm for vegetation patterns. Five saline habitat types, representing variations in soil properties and topography, were identified. Spectral and topomorphometric indices derived from UAV were key to the spatial prediction of soil properties, employing random forest and co-kriging methods. TSC, Na, and pH data served as indicators of salt-affected soils (SAS), and thematic maps were generated for each indicator (57 samples). Overlapping with the vegetation map, the probability range of estimated SAS indicator values was determined. Consequently, a model-based estimation of soil pH, TSC, and Na conditions is provided for habitat types without disturbing protected areas. [ABSTRACT FROM AUTHOR]

Autophagy was originally characterized as a cell survival mechanism in response to starvation, but it is also known as a cell death modulator once the cellular stress becomes irreversible. The initiation and execution of apoptosis occur in three main steps: first, the cells undergoing cell death are specified; next, in the execution phase, apoptosis is activated and regulated; finally, dying cells are eliminated via phagocytosis. A careful balance between cell death and survival is of key importance when it comes to the maintenance of cellular homeostasis. [Extracted from the article]

Background: Each brain hemisphere plays a specialized role in cognitive and behavioral processes, known as hemispheric lateralization. In chronic skin diseases, such as plaque psoriasis (Pso) and atopic dermatitis (AD), the degree of lateralization between the frontal hemispheres may provide insight into specific connections between skin diseases and the psyche. This study aims to analyze the hemispherical lateralization, neurovegetative responses, and psychometric characteristics of patients with Pso and AD. Methods: The study included 46 patients with Pso, 56 patients with AD, and 29 healthy control (Ctrl) subjects. The participants underwent frontal electroencephalogram (EEG) measurement, heart rate variability (HRV) assessment, and psychological tests. Statistical analyses were performed using ANOVA, with Bonferroni correction applied for multiple comparisons. Results: This study shows a significant right-lateralized prefrontal activity in both AD patients (p < 0.001) and Pso patients (p = 0.045) compared with Ctrl, with no significant difference between the AD and Pso groups (p = 0.633). AD patients with right-hemispheric dominant prefrontal activation exhibited increased inhibition and avoidance markers, while Pso patients showed elevated sympathetic nervous system activity. Conclusion: Psychophysiological and psychometric data suggest a shared prevalence of right-hemispheric dominance in both AD and Pso patient groups. However, the findings indicate distinct psychodermatological mechanisms in AD and Pso. [ABSTRACT FROM AUTHOR]

In lipase-catalyzed kinetic resolutions (KRs), the choice of immobilization support and acylating agents (AAs) is crucial. Lipase B from Candida antarctica immobilized onto magnetic nanoparticles (CaLB-MNPs) has been successfully used for diverse KRs of racemic compounds, but there is a lack of studies of the utilization of this potent biocatalyst in the KR of chiral amines, important pharmaceutical building blocks. Therefore, in this work, several racemic amines (heptane-2-amine, 1-methoxypropan-2-amine, 1-phenylethan-1-amine, and 4-phenylbutan-2-amine, (±)-1a–d, respectively) were studied in batch and continuous-flow mode utilizing different AAs, such as diisopropyl malonate 2A, isopropyl 2-cyanoacetate 2B, and isopropyl 2-ethoxyacetate 2C. The reactions performed with CaLB-MNPs were compared with Novozym 435 (N435) and the results in the literature. CaLB-MNPs were less active than N435, leading to lower conversion, but demonstrated a higher enantiomer selectivity, proving to be a good alternative to the commercial form. Compound 2C resulted in the best balance between conversion and enantiomer selectivity among the acylating agents. CaLB-MNPs proved to be efficient in the KR of chiral amines, having comparable or superior properties to other CaLB forms utilizing porous matrices for immobilization. An additional advantage of using CaLB-MNPs is that the purification and reuse processes are facilitated via magnetic retention/separation. In the continuous-flow mode, the usability and operational stability of CaLB-MNPs were reaffirmed, corroborating with previous studies, and the results overall improve our understanding of this potent biocatalyst and the convenient U-shape reactor used. [ABSTRACT FROM AUTHOR]

The fluoroquinolone-type antibiotic ofloxacin is regularly detected in wastewaters. In this work, its hydroxyl radical (•OH) induced degradation was studied at a concentration of 0.1 mmol dm−3 in three water matrices, pure water, tap water, and purified wastewater (collected from a wastewater treatment plant). •OH was produced by γ-irradiation under conditions when it had a high yield. The •OH dosage dependencies of chemical (COD) and biochemical (BOD) oxygen demands, total organic carbon (TOC) content, oxygen uptake rate (OUR), Microtox® toxicity, and antibacterial activity values were evaluated. •OH effectively oxidized and mineralized ofloxacin; both processes were observed even at the lowest •OH dosage, 0.14 mmol dm−3 (0.5 kGy absorbed dose). When 0.28 mmol dm−3 •OH was introduced into pure or tap water, the antibacterial activity vanished. In purified wastewater, a higher dosage (0.56 mmol dm−3) was required to achieve the same effect as in pure water, due to the organic molecules of the matrix that quenched the •OH radicals. The products did not have antibacterial activity and showed little toxicity toward the test organism Vibrio fischeri. •OH preferentially attacks the pharmacophore part, the 4-quinolone unit of ofloxacin. •OH-induced reactions can be safely applied to destroy the antimicrobial potency of fluoroquinolone-type antibiotics in purified wastewater matrices. [ABSTRACT FROM AUTHOR]

Aquatic insects detect water by the horizontal polarization of water-reflected light and thus are attracted to such light. Recently, in the Hungarian Lake Balaton we observed dark water patches forming between every autumn and spring because of the inflow of black suspended/dissolved organic matter into the bright lake water. Earlier, the polarization characteristics of such water surfaces were mapped by imaging polarimeters from the ground. In order to measure the reflection-polarization patterns of these dark lake patches from the higher viewpoint of flying polarotactic aquatic insects, we designed a drone-based imaging polarimeter. We found that the dark lake patches reflected light with very high (60% ≤ d ≤ 80%) degrees of horizontal polarization at the Brewster's angle, while the bright lake water was only weakly (d < 20%) horizontally polarizing. There was a large contrast in both the radiance and degree of polarization between dark lake patches and bright lake water, while there was no such contrast in the angle of polarization. The ecological implication of these findings could be that these dark lake patches attract water-seeking polarotactic insects, which may oviposit more frequently in them than in the brighter lake water. However, it might not matter if they lay their eggs in these dark patches rather than the bright lake water, because this may simply increase the abundance of breeding flying insects in areas where dark patches are common. [ABSTRACT FROM AUTHOR]

Hypocalcemia is a common condition in liver cirrhosis and is associated with the severity of SARS-CoV-2 infection. However, there is a lack of data demonstrating the prognostic value of hypocalcemia in COVID-19 patients with cirrhosis. This study aimed to evaluate the prognostic value of hypocalcemia for COVID-19 severity, mortality and its associations with abnormal liver function parameters. We selected 451 COVID-19 patients in this retrospective study and compared the laboratory findings of 52 COVID-19 patients with cirrhosis to those of 399 COVID-19 patients without cirrhosis. Laboratory tests measuring albumin-corrected total serum calcium were performed on admission, and the levels were monitored during hospitalization. The total serum calcium levels were significantly lower in cirrhosis cases (2.16 mmol/L) compared to those without cirrhosis (2.32 mmol/L). Multivariate analysis showed that hypocalcemia in COVID-19 patients with cirrhosis was a significant predictor of in-hospital mortality, with an OR of 4.871 (p < 0.05; 95% CI 1.566–15.146). ROC analysis showed the AUC value of total serum calcium was 0.818 (95% CI 0.683–0.953, p < 0.05), with a sensitivity of 88.3% and a specificity of 75%. The total serum calcium levels showed a significant negative correlation with the Child–Turcette–Pugh score (r = −0.400, p < 0.05). Hypocalcemia on admission was a significant prognostic factor of disease progression in COVID-19 patients with cirrhosis. [ABSTRACT FROM AUTHOR]

Project Aquafluosense was designed to develop prototypes for a modular fluorescence-based instrumental setup for in situ measurement of major water quality parameters. A fluorometer was developed for algal density estimation based on the fluorescent excitation of chlorophyll. The appropriate type of sample holder microplate was determined, along with the need for dark acclimation, prior to the measurements during the instrument's development. Model species of green (Raphidocelis subcapitata) and blue-green alga (Microcystis aeruginosa) were applied in forms of pure monocultures and their mixtures, and improved analytical limits of detection were achieved (3.70 × 103 cell/mL and 1.13 × 105 for R. subcapitata and M. aeruginosa, respectively). The fluorescence-based determination of algal density was validated by conventional methods, such as cell counting in a Bürker chamber, optical density measurement, and chlorophyll extraction with ethanol. The signals obtained by the fluorometer correlated well with the conventional methods. Pearson r coefficients (applied where the correlation was linear) were ≥0.988 and Spearman ρ coefficients (applied where the correlation was not linear) were >0.976, indicating a strong and positive correlation. The applicability of the developed fluorometer was demonstrated in a growth inhibition ecotoxicity assay on R. subcapitata using the herbicide active ingredient isoxaflutole. During the assay, light intensity (continuous, 104.9 ± 14.9 µE/m2/s), temperature (22 ± 2 °C), pH of algal media (pH = 6–7 for Zehnder and Allen media, as well), and intensity of stirring (continuous, 100 rpm) were controlled. The results indicated that the FluoroMeter Module is applicable for screening algal toxicity: the observed ratio of fluorescence decrease determined by fluorescence induction provided significantly lower toxicity values (EC50: 0.015 ± 0.001 µg/mL) compared to values determined by the optical density (EC50: 0.034 ± 0.004 µg/mL) and chlorophyll a content (EC50: 0.033 ± 0.000 µg/mL). [ABSTRACT FROM AUTHOR]

Maintaining a diverse diet is essential for the preservation of one's health and may contribute to the fight against significant civilization diseases such as obesity or diabetes. Sweet potato can be fitted into a diverse diet and serve as a functional food with its antioxidant content. Therefore, it is important to know how the production technology alters the content and composition of these antioxidant compounds. The objective of this study was to collect information on how the increased potassium dominant fertilizer levels and also the additional high-dose fertigation can affect the phytonutrient contents and yields in an orange- and a purple-fleshed sweet potato cultivar. Field experiments were conducted in the Hungarian county of Heves in two consecutive growing seasons in 2021–2022. Different doses of potassium-predominant fertilizer were applied to an orange flesh (Beauregard) and a purple flesh (Stokes Purple) varieties of sweet potatoes. Different application techniques were used in the form of base and top dressing at different rates. The effect on yield and polyphenol content of the different fertilizer rates was investigated. Analytical studies were carried out by high-performance liquid chromatography (HPLC). In the case of the Beauregard variety, in addition to identifying the carotenoids, we also performed their quantitative determination. We found that 87% of the carotenoid content was ß-carotene. The total anthocyanin content was investigated for the purple variety—Stokes Purple, for which a new extraction method was developed. In our study, the split dosages, when the pre-planting fertilizer was supplemented with additional liquid fertilization, resulted in 36 and 30.5% higher yields in the Beauregard in Experiment I and Experiment II, respectively, compared to the untreated control plots over the two years. The additional liquid fertilizer increased the yield to a lesser extent when compared to treatments received only pre-planting fertilizer. As for Experiment III, the split dosages resulted in 34.4% higher yields in the Stokes Purple compared to the control plots. However, the additional liquid fertilizer was not effective at all when the plants received a double dose of pre-planting fertilizer in the case of Stokes Purple. Total carotenoid (Experiment I–II) was higher and anthocyanin yield per plant (Experiment III) was significantly higher in the split-dosage treatment than in the untreated control plots. [ABSTRACT FROM AUTHOR]

Among vector-borne protozoa Hepatozoon felis and Cytauxzoon europaeus are considered emerging species in felids in Europe. To investigate the presence of these two protozoa 127 domestic cats and 4 wildcats were screened by PCRs targeting the 18S rRNA gene of Hepatozoon spp. and piroplasms, as well as the cytb gene of Cytauxzoon spp. The samples were collected inside and outside a region of Hungary, where both protozoan groups are endemic in wildcats. Among domestic cats, one proved to be infected with H. felis. Furthermore, spleen samples of four wildcats were also examined, among which three tested positive for H. felis, and one had co-infection with C. europaeus. Importantly, H. felis from the co-infected wildcat belonged to genogroup II, similarly to H. felis from the positive domestic cat. Based on phylogenetic evidence, this genogroup probably represents a separate species from genogroup I of H. felis, which was hitherto reported from Mediterranean countries in Europe. The two other wildcats also harbored H. felis from genogroup I. Neither Hepatozoon nor Cytauxzoon infections were detected outside the recently discovered endemic region. In conclusion, this study demonstrates for the first time in Europe that H. felis from genogroup II may emerge in free-roaming domestic cats in regions where this protozoan parasite is endemic in wildcats. [ABSTRACT FROM AUTHOR]