3,134 results on '"Suárez A"'
Search Results
2. Uptake and completion of tuberculosis preventive treatment using 12-dose, weekly isoniazid-rifapentine regimen in Bangladesh: A community-based implementation study
- Author
-
Rahman, Md Toufiq, Hossain, Farzana, Banu, Rupali Sisir, Islam, Md Shamiul, Alam, Shamsher, Faisel, Abu Jamil, Hussain, Hamidah, Roy, Tapash, Salim, Hamid, Cordon, Oscar, and Suarez, Pedro
- Published
- 2024
3. Clinical characteristics associated with detected respiratory microorganism employing multiplex nested PCR in patients with presumptive COVID-19 but negative molecular results in Lima, Peru
- Author
-
de la Torre Pretell, Juan Carlos Gomez, Hueda-Zavaleta, Miguel, Caceres-DelAguila, Jose Alonso, Barletta-Carrillo, Claudia, Copaja-Corzo, Cesar, Suarez Poccorpachi, Maria del Pilar, Delgado, Maria Soledad Vega, Levano Sanchez, Gloria Maria Magdalena, and Benites-Zapata, Vicente A
- Published
- 2022
4. Monkeypox and HIV in the canary islands: A different pattern in a mobile population
- Author
-
Betancort-Plata, Christian, Lopez-Delgado, Laura, Jaen-Sanchez, Nieves, Tosco-Nunez, Tomas, Suarez-Hormiga, Laura, Lavilla-Salgado, Carmen, Pisos-Alamo, Elena, Hernandez-Betancor, Araceli, Hernandez-Cabrera, Michele, Carranza-Rodriguez, Cristina, Briega-Molina, Marta, and Perez-Arellano, Jose-Luis
- Published
- 2022
5. Study of the Chemical Recovery and Selectivity against U in the Radiochemical Separation of Th with Tri-n-butyl Phosphate by Varying the Proportion of Xylene and HCl Concentration.
- Author
-
Expósito-Suárez, Víctor Manuel, Suárez-Navarro, José Antonio, and Benavente, José Francisco
- Subjects
- *
FUEL cycle , *CHEMICAL yield , *COMPLEX matrices , *ENVIRONMENTAL sciences , *THORIUM , *THORIUM isotopes , *LIQUID-liquid extraction - Abstract
Thorium is a radionuclide used in various environmental studies such as dating, sediment movement, soil–plant transfer studies, and contamination of waste from the natural fuel cycle. The liquid–liquid extraction method using tri-n-butyl phosphate (TBP) allows for the separation of Th from the accompanying actinides. However, the separation of Th and U present in the same sample is not trivial. This separation is influenced by the starting acid (HCl or HNO3), the concentration of TBP in an organic solvent, and the concentration of the acid used for re-extracting Th, which is typically HCl. Therefore, it is necessary to study these factors to ensure that the method has sufficient chemical yield and selectivity in complex matrices. This study presents a systematic investigation of the aforementioned parameters, making the necessary variations to select an optimal method for the radiochemical separation of Th. The ideal conditions were obtained using 4 M HCl as the acid prior to extraction, a 1:4 solution of TBP in xylene, and 4 M HCl as the re-extracting agent. The accuracy and precision were studied in four intercomparison exercises conducted in quadruplicate, using the parameters Enumbers, RB(%), and RSD(%) for 232Th and 230Th. The sensitivity of the method was experimentally studied and the limit of detection (LoD) was determined according to ISO 11929:2005. Additionally, the linearity of the method showed that the experimental and theoretical activity concentrations of 232Th and 230Th had slopes of 1 with an intercept close to 0. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. Harnessing Bacillus amyloliquefaciens for Amazake Production: Comparison with Aspergillus oryzae Amazake for Metabolomic Characteristics, Microbial Diversity, and Sensory Profile.
- Author
-
Touceda-Suárez, Alejandra, Touceda-Suárez, María, Arboleya, Juan-Carlos, and Sörensen, Pia M.
- Subjects
FERMENTED beverages ,BACILLUS amyloliquefaciens ,KOJI ,MICROBIAL diversity ,METABOLOMICS ,FERMENTED foods ,SHIKIMIC acid - Abstract
Amazake is a traditional, sweet, non-alcoholic Japanese beverage typically produced through koji fermentation by the fungus Aspergillus oryzae. However, alternative microorganisms such as Bacillus amyloliquefaciens offer potential advantages and novel possibilities for producing similar fermented beverages. This study aimed to replicate the ancestral beverage of amazake by replacing A. oryzae (W-20) with B. amyloliquefaciens (NCIMB 12077) and comparing their fermentation processes and resulting products. Our results show that the production of amazake with B. amyloliquefaciens (ABA) is not only possible but also results in a beverage that is otherwise distinct from traditional amazake (AAO). Saccharification was achievable in ABA at higher temperatures than in AAO, albeit with lower reducing sugar and enzymatic activity values. Amino acids and organic acids were more abundant in AAO, with cysteine being uniquely present in AAO and shikimic acid only being present in ABA. The volatile aroma compound profiles differed between the two beverages, with AAO exhibiting a greater abundance of aldehydes, and ABA a greater abundance of ketones and alcohols. Interestingly, despite these compositional differences, the two beverages showed similar consumer panel acceptance rates. An analysis of their microbial communities revealed pronounced differences between the amazakes, as well as temporal changes in ABA but not in AAO. This study provides promising insights into harnessing the potential of B. amyloliquefaciens as the primary microorganism in the fermentation process of amazake-like beverages, marking an important advancement in the field of fermented low-alcohol beverage production, with possible applications in other fermented foods. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
7. Nutrient Contribution and Carbon Sequestration of an Agroforestry System of Coffea canephora Cultivated by Conventional and Organic Management in the Ecuadorian Amazon.
- Author
-
Tinoco-Jaramillo, Leider, Vargas-Tierras, Yadira, Paredes-Arcos, Fernando, Viera, William, Suárez-Tapia, Alfonso, Vargas-Tierras, Tannia, Suárez-Cedillo, Sandra, Morales-León, Vanessa, and Vásquez-Castillo, Wilson
- Subjects
CARBON sequestration ,AGROFORESTRY ,COFFEE ,COFFEE growing ,SECONDARY forests ,SOIL depth - Abstract
Agroforestry systems (AFSs) seek synergies that improve productivity, sustainability, and environmental benefits. This is achieved through the supplying of nutrients to the soil, carbon storage, and sequestration. In the Ecuadorian Amazon, Coffea canephora is planted together with leguminous, woody, forest, and secondary forest species, where the continuous incorporation of vegetative residues from shade species represents a substantial addition of nutrients within these systems. This study was carried out from 2018 to 2022 to determine the contribution of nutrients contained in the biomass and C sequestration in agroforestry systems of coffee with conventional (high use of agrochemicals) and organic (without the use of chemicals) management. The study was carried out with a randomized complete block design, using a factorial arrangement (2 × 4 with three replications). This arrangement included two types of systems (agroforestry and monoculture) and four agronomic management practices (high and medium for conventional, and intensive and low organic). The biomass and nutrient content were measured twice a year (every 180 days); in addition, the yield was also recorded. A multivariate and univariate analysis was used for data analysis through R and SAS software. After five years of evaluation, it was determined that the N, K, Ca, and Mg contents were higher in the agroforestry systems than the monocultures. In the AFSs, the highest nutrient content was obtained with the medium conventional and low organic agronomic management, while in the monocultures, it was obtained with the high and medium conventional management. In addition, at a soil depth of 20 cm, the total storage and CO
2 were 38.12 and 139.8 t ha−1 , respectively. The highest yields were obtained with conventional management in AFSs (1599 kg ha−1 ) and monoculture (1789.45 kg ha−1 ). Overall, AFSs showed a significant contribution of nutrients, such as N, K, Ca, and Mg, for coffee cultivation; moreover, yields were similar in the AFS and monoculture with both conventional and organic management, which is positive, since AFSs also contribute environmental benefits. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
8. Antimigratory Effect of Lipophilic Cations Derived from Gallic and Gentisic Acid and Synergistic Effect with 5-Fluorouracil on Metastatic Colorectal Cancer Cells: A New Synthesis Route.
- Author
-
Suárez-Rozas, Cristian, Jara, José Antonio, Cortés, Gonzalo, Rojas, Diego, Araya-Valdés, Gabriel, Molina-Berrios, Alfredo, González-Herrera, Fabiola, Fuentes-Retamal, Sebastián, Aránguiz-Urroz, Pablo, Campodónico, Paola Rossana, Maya, Juan Diego, Vivar, Raúl, and Catalán, Mabel
- Subjects
- *
CELL migration inhibition , *IN vitro studies , *CELL migration , *VASCULAR endothelial growth factors , *MITOCHONDRIA , *RESEARCH funding , *APOPTOSIS , *COLORECTAL cancer , *CELLULAR signal transduction , *AMP-activated protein kinases , *REVERSE transcriptase polymerase chain reaction , *DESCRIPTIVE statistics , *METASTASIS , *CELL lines , *PLANT extracts , *CELL culture , *CYTOTOXINS , *PHENOLS , *MOLECULAR structure , *ORGANOPHOSPHORUS compounds , *MICROBIOLOGICAL assay , *WESTERN immunoblotting , *MATRIX metalloproteinases , *ONE-way analysis of variance , *FLUOROURACIL , *CELL survival , *DATA analysis software , *DISEASE progression - Abstract
Simple Summary: Colorectal cancer (CRC) is one of the most common causes of death worldwide. Today, this disease does not have an effective treatment, leading to the exploration of novel pharmacological molecules. In this paper, we design and synthesize with a new synthetic route the lipophilic cation derived from gallic acid (TPP+C10) and gentisic acid (GA-TPP+C10), both able to reach mitochondria and uncouple the electron transport chain. Our results show that combining 5-fluorouracil with GA-TPP+C10 has a synergistic cytotoxic effect on CRC cells. Both compounds show antimigratory effects, decreasing signaling pathways and biomarkers. Our results show that mitochondrial agents could be an alternative to standard CRC drugs against this disease. Colorectal cancer (CRC) is the third leading cause of cancer deaths in the world. Standard drugs currently used for the treatment of advanced CRC—such as 5-fluorouracil (5FU)—remain unsatisfactory in their results due to their high toxicity, high resistance, and adverse effects. In recent years, mitochondria have become an attractive target for cancer therapy due to higher transmembrane mitochondrial potential. We synthesized gallic acid derivatives linked to a ten-carbon aliphatic chain associated with triphenylphosphonium (TPP+C10), a lipophilic cationic molecule that induces the uncoupling of the electron transport chain (ETC). Other derivatives, such as gentisic acid (GA-TPP+C10), have the same effects on colorectal cancer cells. Although part of our group had previously reported preparing these structures by a convergent synthesis route, including their application via flow chemistry, there was no precedent for a new methodology for preparing these compounds. In this scenario, this study aims to develop a new linear synthesis strategy involving an essential step of Steglich esterification under mild conditions (open flask) and a high degree of reproducibility. Moreover, the study seeks to associate GA-TPP+C10 with 5FU to evaluate synergistic antineoplastic effects. In addition, we assess the antimigratory effect of GA-TPP+C10 and TPP+C10 using human and mouse metastatic CRC cell lines. The results show a new and efficient synthesis route of these compounds, having synergistic effects in combination with 5FU, increasing apoptosis and enhancing cytotoxic properties. Additionally, the results show a robust antimigratory effect of GATPP+C10 and TPP+C10, reducing the activation pathways linked to tumor progression and reducing the expression of VEGF and MMP-2 and MMP-9, common biomarkers of advanced CRC. Moreover, TPP+C10 and GA-TPP+C10 increase the activity of metabolic signaling pathways through AMPK activation. The data allow us to conclude that these compounds can be used for in vivo evaluations and are a promising alternative associated with conventional therapies for advanced colorectal cancer. Additionally, the reported intermediates of the new synthesis route could give rise to analog compounds with improved therapeutic activity. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
9. Constitutional Factors and Irradiation as Risk Factors for Thymoma: A European Case–Control Study.
- Author
-
Kaerlev, Linda, Eriksson, Mikael, Guénel, Pascal, Merletti, Franco, Morales-Suárez-Varela, Maria, Ahrens, Wolfgang, Jöckel, Karl-Heinz, Llopis-Gonzalez, Agustin, Wingren, Gun, and Simonato, Lorenzo
- Published
- 2024
- Full Text
- View/download PDF
10. Effect of Layout Discretization on the Performance of Zone Control-Based Multi-AGV Traffic Management Systems.
- Author
-
Verma, Parikshit, Olm, Josep M., and Suárez, Raúl
- Subjects
FLEXIBLE manufacturing systems ,MANUFACTURING processes ,MATERIALS handling ,AUTOMATED guided vehicle systems ,NETWORK performance - Abstract
Automatic Guided Vehicles (AGVs) are widely used in flexible manufacturing systems for material handling inside the factory. Traffic management strategies, required to guarantee a conflict-free operation of the overall fleet, discretize the workspace of the AGVs and use the resulting graph model for route planning and execution. In zone control approaches, AGVs move from node to node on a permit basis, with limitations on the allowed number of AGVs at a time in each area of the graph to prevent and/or resolve deadlocks and conflicts. Hence, for an optimal implementation of traffic controllers in real manufacturing systems, it is essential to understand how the layout discretization influences the performance of the AGV network. This paper analyzes its effect in grid-like shaped workspaces by using a representative zone control algorithm and a recently developed improvement of it. Realistic numerical experiments on different layouts reveal that denser discretizations do not yield faster executions or increase in throughput, while lower control periods in the permit system entail significant performance uplifts. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
11. Neuromuscular Load in Professional Women's Handball: Segmentation of the Player Load and the Impacts at Group and Individual Level.
- Author
-
Antúnez, Antonio, López-Sierra, Pablo, Vila-Suárez, Helena, and Ibáñez, Sergio J.
- Subjects
TEAM sports ,IMPACT loads ,BUSINESSWOMEN ,HANDBALL players ,HANDBALL - Abstract
Handball is a team sport characterised by physical interaction with other opponents. This interaction produces a high load on the players that can manifest itself in various ways, from discomfort to prolonged injuries due to tears caused by excessive load. In order to establish correct protocols for application in women's teams, context- and gender-specific reference data must be available. For this reason, the present research aims to find out how women's teams in European competitions prepare for decisive matches during the match week, analysing the load in a segmented way and the level of specificity that should be achieved in training. Ex post facto research was used in which a total of 17 players belonging to a women's first division handball team in Spain participated. The variables player load and impacts extracted from the use of Wimu ProTM inertial devices were analysed. The results showed a high neuromuscular load in players at this competitive level, especially in the variable impacts, reaching values per session of up to 1000 impacts. The individuality analyses show that the load varies significantly depending on the subject, which is why it is considered essential to establish protocols for strength work and load control in the most specific way possible. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
12. Mitigating Cold Ischemic Injury: HTK, UW and IGL-2 Solution's Role in Enhancing Antioxidant Defence and Reducing Inflammation in Steatotic Livers.
- Author
-
Bardallo, Raquel G., Chullo, Gabriela, Alva, Norma, Rosello-Catafau, Joan, Fundora-Suárez, Yiliam, Carbonell, Teresa, and Panisello-Rosello, Arnau
- Subjects
FATTY liver ,PRESERVATION of organs, tissues, etc. ,LABORATORY rats ,LIVER transplantation ,REACTIVE oxygen species ,GLUTATHIONE peroxidase ,SUPEROXIDE dismutase - Abstract
Liver transplantation remains the only definitive treatment for end-stage liver diseases. However, the increasing prevalence of fatty liver disease among potential donors exacerbates the shortage of suitable organs. This study evaluates the efficacy of the preservation solution Institut Georges Lopez-2 (IGL-2) compared to Histidine–Tryptophan–Ketoglutarate (HTK) and University of Wisconsin (UW) preservation solutions in mitigating ischemia-reperfusion injury (IRI) in steatotic livers. Using Zucker Obese rat livers, we assessed the impact of 24-h static cold storage (SCS) with each solution on transaminase release, glutathione redox balance, antioxidant enzyme activity, lipoperoxidation, and inflammation markers. IGL-2 and UW solutions demonstrated reduced transaminase and lactate levels compared to HTK, indicating better preservation of liver integrity. IGL-2 maintained a higher reduced glutathione/oxidized glutathione (GSH/GSSG) ratio, suggesting more effective management of oxidative stress. Antioxidant enzyme activities catalase, superoxide dismutase, and glutathione peroxidase (CAT, SOD, GPX) were higher in IGL-2 preserved livers, contributing to decreased oxidative damage. Lipid peroxidation markers and inflammatory markers were lower in IGL-2 than in HTK, indicating reduced oxidative stress and inflammation. Additionally, improved mitochondrial function was observed in the IGL-2 group, correlating with reduced reactive oxygen species (ROS) production and lipid peroxidation. These findings suggest that IGL-2 offers superior preservation of liver viability, reduces oxidative stress, and minimizes inflammation compared to HTK and UW solutions. By maintaining a higher ratio of reduced glutathione and antioxidant enzyme activity, IGL-2 effectively mitigates the harmful effects of ischemia-reperfusion injury. The reduced lipid peroxidation and inflammation in the IGL-2 group further underscore its potential in improving liver transplant outcomes. These results highlight the importance of optimizing preservation solutions to enhance the viability and functionality of donor organs, potentially expanding the donor pool and improving the success rates of liver transplantation. Future research should focus on refining preservation techniques and exploring additional protective agents to further improve organ preservation and transplant outcomes. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. The Anatomy, Histology, and Function of the Major Pelvic Ganglion.
- Author
-
Landa-García, Jessica Natalia, Palacios-Arellano, María de la Paz, Morales, Miguel Angel, Aranda-Abreu, Gonzalo Emiliano, Rojas-Durán, Fausto, Herrera-Covarrubias, Deissy, Toledo-Cárdenas, María Rebeca, Suárez-Medellín, Jorge Manuel, Coria-Avila, Genaro Alfonso, Manzo, Jorge, and Hernández-Aguilar, Maria Elena
- Subjects
CALCITONIN gene-related peptide ,NEUROPEPTIDE Y ,VASOACTIVE intestinal peptide ,SUBSTANCE P ,HOMOLOGY (Biology) - Abstract
Simple Summary: In male rats, the major pelvic ganglion is the principal component of the pelvic plexus and plays a crucial role in regulating various physiological functions such as urination, defecation, erection, ejaculation, and glandular secretion. This ganglion is considered mixed, as it receives both sympathetic and parasympathetic innervation through the hypogastric and pelvic nerves, respectively. Homologous structures with similar functions are present in other species, including cats, dogs, and pigs; however, differences exist in nomenclature, anatomical complexity, and functionality. Although anatomical, histological, and immunohistochemical studies have been conducted on these structures across various species, the major pelvic ganglion of the rat has been the most extensively studied due to its ease of identification and manipulation. This review provides a comprehensive analysis of the pelvic plexus and its regulation across various mammalian species, including rats, cats, dogs, and pigs. The pelvic and hypogastric nerves play crucial roles in regulating pelvic functions such as micturition, defecation, and erection. The anatomical organization of these nerves varies, forming either well-defined ganglia or complex plexuses. Despite these variations, the neurons within these structures are consistently regulated by key neurotransmitters, norepinephrine and acetylcholine. These neurons also possess receptors for testosterone and prolactin, particularly in rats, indicating the significant role of these hormones in neuronal function and development. Moreover, neuropeptides such as vasoactive intestinal peptide (VIP), substance P, neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), and calcitonin gene-related peptide (CGRP) are co-released with neurotransmitters to modulate pelvic functions. This review highlights the complex interplay between neurotransmitters, neuropeptides, and hormones in regulating pelvic physiology and emphasizes the importance of hormonal regulation in maintaining the functionality and health of the pelvic plexus across different species. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
14. Association of Polyamine Intake, Other Dietary Components, and Fecal Content of N -acetyl Putrescine and Cadaverine with Patients' Colorectal Lesions.
- Author
-
Barreiro-Alonso, Eva, Castro-Estrada, Paula, Sánchez, Manuel, Peña-Iglesias, Pablo, Suárez, Lorena, and Cantabrana, Begoña
- Abstract
Colorectal cancer (CRC) is the second leading cause of cancer death worldwide. Early detection and the modification of risk factors, such as diet, can reduce its incidence. Among food components, polyamines are important for maintaining gastrointestinal health and are metabolites of gut microbiota. Their disruption is linked to CRC, making polyamines a potential marker of the disease. This study analyzed the relationship between dietary components, including polyamines, and the presence of polyamines in feces to determine whether their presence could contribute to predicting the occurrence of colorectal lesions in patients. In total, 59 participants of both sexes (aged 50 to 70 years) who had undergone colonoscopy screening for CRC (18 without and 41 with colorectal lesions) participated in the study. A nutritional survey and determination of fecal polyamine content were performed. Specific dietary components and putrescine levels were higher in patients with colorectal lesions. The diet ratio of putrescine–spermidine and the fecal content of N-acetyl putrescine and cadaverine were elevated in patients with precancerous lesions and adenocarcinomas, showing a potential predictive value for the presence of colorectal lesions. These findings suggest that N-acetyl putrescine and cadaverine could be complementary markers for the diagnosis of suspected colorectal lesions. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
15. IA-Body Composition CT at T12 in Idiopathic Pulmonary Fibrosis: Diagnosing Sarcopenia and Correlating with Other Morphofunctional Assessment Techniques.
- Author
-
Fernández-Jiménez, Rocío, Sanmartín-Sánchez, Alicia, Cabrera-César, Eva, Espíldora-Hernández, Francisco, Vegas-Aguilar, Isabel, Amaya-Campos, María del Mar, Palmas-Candia, Fiorella Ximena, Claro-Brandner, María, Olivares-Alcolea, Josefina, Simón-Frapolli, Víctor José, Cornejo-Pareja, Isabel, Guirado-Peláez, Patricia, Vidal-Suárez, Álvaro, Sánchez-García, Ana, Murri, Mora, Garrido-Sánchez, Lourdes, Tinahones, Francisco J., Velasco-Garrido, Jose Luis, and García-Almeida, Jose Manuel
- Abstract
Background: Body composition (BC) techniques, including bioelectrical impedance analysis (BIVA), nutritional ultrasound
® (NU), and computed tomography (CT), can detect nutritional diagnoses such as sarcopenia (Sc). Sc in idiopathic pulmonary fibrosis (IPF) is associated with greater severity and lower survival. Our aim was to explore the correlation of BIVA, NU and functional parameters with BC at T12 level CT scans in patients with IPF but also its relationship with degree of Sc, malnutrition and mortality. Methods: This bicentric cross-sectional study included 60 IPF patients (85.2% male, 70.9 ± 7.8 years). Morphofunctional assessment (MFA) techniques included BIVA, NU, CT at T12 level (T12-CT), handgrip strength, and timed up and go. CT data were obtained using FocusedON® . Statistical analysis was conducted using JAMOVI version 2.3.22 to determine the cutoff points for Sc in T12-CT and to analyze correlations with other MFA techniques. Results: the cutoff for muscle area in T12-CT was ≤77.44 cm2 (area under the curve (AUC) = 0.734, sensitivity = 41.7%, specificity = 100%). The skeletal muscle index (SMI_T12CT) cutoff was ≤24.5 cm2 /m2 (AUC = 0.689, sensitivity = 66.7%, specificity = 66.7%). Low SMI_T12CT exhibited significantly reduced median survival and higher risk of mortality compared to those with normal muscle mass (SMI cut off ≥ 28.8 cm/m2 ). SMI_T12CT was highly correlated with body cell mass from BIVA (r = 0.681) and rectus femoris cross-sectional area (RF-CSA) from NU (r = 0.599). Cronbach's α for muscle parameters across different MFA techniques and CT was 0.735, confirming their validity for evaluating muscle composition. Conclusions: T12-CT scan is a reliable technique for measuring low muscle mass in patients with IPF, specifically when the L3 vertebrae are not captured. An SMI value of <28.8 is a good predictor of low lean mass and 12-month mortality in IPF patients. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
16. Epidemiology of Neuro-Behçet's Disease in Northern Spain 1999–2019: A Population-Based Study.
- Author
-
Herrero-Morant, Alba, Martín-Varillas, José Luis, Álvarez-Reguera, Carmen, Sánchez-Bilbao, Lara, Martínez-López, David, Suárez-Amorín, Guillermo, Fernández-Ramón, Raúl, Ferraz-Amaro, Iván, Castañeda, Santos, Hernández, José L., and Blanco, Ricardo
- Subjects
BEHCET'S disease ,NEUROLOGICAL disorders ,COHORT analysis ,GENDER ,EPIDEMIOLOGY - Abstract
Background/Objectives: Neuro-Behçet's disease (NBD) is one of the most severe complications of Behçet's disease (BD). The incidence of NBD varies widely worldwide. This study aimed to estimate its current incidence in Northern Spain. Methods: This was a retrospective population-based cohort study of 120 patients in Northern Spain diagnosed with BD according to the 2013 International Criteria for BD (ICBD) between 1 January 1999 and 31 December 2019. NBD diagnoses were made according to International Consensus Recommendation (ICR) criteria. Overall, 96 patients were included, and their demographic and clinical data were collected. The incidence of NBD was estimated by age, gender, and year of diagnosis between 1999–2019. Results: NBD was diagnosed in 23 of 96 (24%) patients (15 women/8 men) (mean age: 44 ± 13.9 years). HLA-B51 was positive in 5 of 13 (38.4%) cases tested. A total of 10 (43.5%) patients had parenchymatous NBD, 10 (43.5%) had non-parenchymatous NBD, and 3 (13%) had mixed NBD. Incidence during the study period was 0.13 (95% CI, 0.11–0.26) per 100,000 people-years. There were no significant differences in gender in the incidence rate stratified by age (p > 0.05). Furthermore, there was a linear relationship with a mild decrease in age at diagnosis over time. Conclusions: Epidemiological characteristics of NBD in Northern Spain are similar to those of neighboring countries, except female gender predominance. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
17. Utility of Stool-Based Tests for Colorectal Cancer Detection: A Comprehensive Review.
- Author
-
Gómez-Molina, Raquel, Suárez, Miguel, Martínez, Raquel, Chilet, Marifina, Bauça, Josep Miquel, and Mateo, Jorge
- Subjects
MORTALITY prevention ,FECAL analysis ,IMMUNOCHEMISTRY ,EARLY detection of cancer ,COLORECTAL cancer ,DIAGNOSTIC errors ,CLINICAL pathology ,FECAL occult blood tests ,BIOMARKERS - Abstract
Colorectal cancer (CRC) is a significant global health issue where early detection is crucial for improving treatment outcomes and survival rates. This comprehensive review assesses the utility of stool-based tests in CRC screening, including traditional fecal occult blood tests (FOBT), both chemical (gFOBT) and immunochemical techniques (FIT), as well as multitarget stool DNA (mt-sDNA) as a novel and promising biomarker. The advancements, limitations and the impact of false positives and negatives of these methods are examined. The review analyzed various studies on current screening methods, focusing on laboratory tests and biomarkers. Findings indicate that while FIT and mt-sDNA tests offer enhanced sensitivity and specificity over traditional guaiac-based FOBT, they also come with higher costs and potential for increased false positives. FIT shows better patient adherence due to its ease to use, but incorrect usage and interpretation of FOBT can lead to significant diagnostic errors. In conclusion, despite the improvements in FOBT methods like FIT in CRC detection, careful consideration of each method's benefits and drawbacks is essential. Effective CRC screening programs should combine various methods tailored to specific population needs, aiming for early detection and reduced mortality rates. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
18. Numerical Modeling for Tunnel Lining Optimization.
- Author
-
Suárez-Fino, Jose Francisco and Mayoral, Juan M.
- Subjects
TUNNEL design & construction ,TUNNEL lining ,FINITE differences ,TUNNELS ,URBAN planning - Abstract
The construction of tunnels excavated by the conventional method in densely populated urban environments requires an adequate characterization of the loads acting on the primary lining during the excavation process, to ensure that the ground is deformed and stresses around the tunnel are relieved, simultaneously complying with the failure and serviceability limits of international standards while minimizing damage to nearby structures. In this paper, common lining design criteria are revisited, through the numerical simulation of an instrumented tunnel section which is part of a 4.5 km long metro line currently under construction in Mexico City. Key needs for improvement in current design approaches are identified. The tunnel was instrumented with load cells, extensometers, and topographical references for convergences and divergences. A three-dimensional finite difference model of the instrumented section was developed, and the load transfer mechanisms between the excavated soil and the primary lining were analyzed. Then, the numerical simulation of the contribution of the secondary lining in the overall stability for sustained load was established, along with the expected ground settlements, which can significantly affect nearby structures. Results gathered from this research are key for updating lining design criteria for urban tunnels built in stiff brittle soils. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
19. Cross-Sectional Assessment of Nutritional Status, Dietary Intake, and Physical Activity Levels in Children (6–9 Years) in Valencia (Spain) Using Nutrimetry.
- Author
-
Morales-Suárez-Varela, María, Peraita-Costa, Isabel, Llopis-Morales, Agustín, and Llopis-González, Agustín
- Abstract
The aims of this research were to evaluate the current nutritional status, dietary intake, and level of physical activity and assess the need for intervention. This was a cross-sectional study with 2724 participating children aged 6–9 years old. Nutritional status was assessed using nutrimetry, dietary intake with a 3-day food-recall questionnaire and physical activity with an ad hoc questionnaire. The nutricode with the highest prevalence was healthy weight/normal stature, with 51.3% of the sample. For the BMI for age Z-score, those in the overweight/obesity category represented 37.5% of the sample, while the thinness category included 7.6%. Intake of calories, proteins, sugar, lipids, SFA, MUFA, and cholesterol were significantly higher than recommended. The thinness groups consumed a significantly higher amount of excess calories while the overweight/obesity groups had the lowest mean excess calorie intake. Children in the thinness category presented the highest rates at both ends of the spectrum for sedentary activities. This study showed the high prevalence of malnutrition in schoolchildren. The results for the risk of thinness and overweight/obesity according to individual nutrient intake should be carefully interpreted. Lifestyle is a fundamental aspect to consider when combating malnutrition, especially at the level of dietary and physical activity habits, to combine various methods of intervention to improve nutritional status. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
20. Dietary Flavonoids: Mitigating Air Pollution's Cardiovascular Risks.
- Author
-
Rocha-Velasco, Oscar Andrés, Morales-Suárez-Varela, María, and Llopis-González, Agustín
- Abstract
Air pollution significantly impacts cardiovascular health, yet pollution reduction strategies in cardiovascular disease prevention remain limited. Dietary flavonoids show promise in protecting cardiovascular health, but their potential to mitigate air-pollution-induced risks is unexplored. This study investigates this research gap. Following PRISMA-ScR guidelines, literature from 2014–2024 was searched across MedLine/PubMed, ScienceDirect, and MDPI databases. Of 463 identified studies, 53 were eligible for analysis based on PICO criteria. Findings revealed significant impacts of air pollution on cardiovascular health, including increased disease risks and mortality. Flavonoid intake demonstrated protective effects against these risks. Flavonoid mechanisms include improved endothelial function, antioxidant and anti-inflammatory effects, blood pressure regulation, antiplatelet effects, cardioprotection, and enhanced lipid and glucose metabolism. Higher flavonoid intake was consistently associated with reduced cardiovascular risks. While reducing pollution remains crucial, promoting flavonoid-rich diets is a promising complementary strategy. Public health initiatives should raise awareness about these benefits. Further research on direct interactions between flavonoid intake and air pollution exposure is needed. Current evidence supports integrating dietary interventions into broader strategies to reduce air pollution's cardiovascular impacts. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
21. Novel Criteria to Provide a Locality/Normality Degree in Molecules and Their Relevance in Physical Chemistry.
- Author
-
Suárez, Eduardo, Guzmán-Juárez, Oscar, and Lemus, Renato
- Subjects
- *
PHYSICAL & theoretical chemistry , *RAMAN spectroscopy , *INFRARED spectroscopy , *CONFIGURATION space , *QUANTUM numbers , *ISOTOPOLOGUES - Abstract
In contrast to the traditional analysis of molecules using local mode behavior, where the degree of locality is given through a function in terms of Morse potential parameters, new criteria for locality/normality (LN) suitable for application to any molecular system are proposed. The approach is based on analysis of the connection between the algebraic normal and local mode representations. It is shown that both descriptions are equivalent as long as the polyad (total number of quanta) in the local representation is not conserved. The constraint of a local polyad conservation naturally provides a criterion for assigning an LN degree in quantitative form, without an analogue in configuration space. The correlation between the different parameters reveals the physical properties of molecules. A clear connection between the LN degree (based on the fundamentals) and spectroscopic properties is also presented, suggesting a promising approach for identifying mixtures of isotopologues. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
22. Assessment of Albumin-Incorporating Scores at Hepatocellular Carcinoma Diagnosis Using Machine Learning Techniques: An Evaluation of Prognostic Relevance.
- Author
-
Suárez, Miguel, Martínez-Blanco, Pablo, Gil-Rojas, Sergio, Torres, Ana M., Torralba-González, Miguel, and Mateo, Jorge
- Subjects
- *
HEPATOCELLULAR carcinoma , *MACHINE learning , *LIVER cancer , *DIAGNOSIS , *C-reactive protein , *TRAUMA registries - Abstract
Hepatocellular carcinoma (HCC) presents high mortality rates worldwide, with limited evidence on prognostic factors at diagnosis. This study evaluates the utility of common scores incorporating albumin as predictors of mortality at HCC diagnosis using Machine Learning techniques. They are also compared to other scores and variables commonly used. A retrospective cohort study was conducted with 191 patients from Virgen de la Luz Hospital of Cuenca and University Hospital of Guadalajara. Demographic, analytical, and tumor-specific variables were included. Various Machine Learning algorithms were implemented, with eXtreme Gradient Boosting (XGB) as the reference method. In the predictive model developed, the Barcelona Clinic Liver Cancer score was the best predictor of mortality, closely followed by the Platelet-Albumin-Bilirubin and Albumin-Bilirubin scores. Albumin levels alone also showed high relevance. Other scores, such as C-Reactive Protein/albumin and Child-Pugh performed less effectively. XGB proved to be the most accurate method across the metrics analyzed, outperforming other ML algorithms. In conclusion, the Barcelona Clinic Liver Cancer, Platelet-Albumin-Bilirubin and Albumin-Bilirubin scores are highly reliable for assessing survival at HCC diagnosis. The XGB-developed model proved to be the most reliable for this purpose compared to the other proposed methods. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
23. New Test to Detect Clustered Graphical Passwords in Passpoints Based on the Perimeter of the Convex Hull.
- Author
-
Herrera-Macías, Joaquín Alberto, Suárez-Plasencia, Lisset, Legón-Pérez, Carlos Miguel, Sosa-Gómez, Guillermo, and Rojas, Omar
- Subjects
- *
FALSE positive error , *CONVEX sets , *TEST design , *SECURITY systems , *TIME management , *CHI-squared test - Abstract
This research paper presents a new test based on a novel approach for identifying clustered graphical passwords within the Passpoints scenario. Clustered graphical passwords are considered a weakness of graphical authentication systems, introduced by users during the registration phase, and thus it is necessary to have methods for the detection and prevention of such weaknesses. Graphical authentication methods serve as a viable alternative to the conventional alphanumeric password-based authentication method, which is susceptible to known weaknesses arising from user-generated passwords of this nature. The test proposed in this study is based on estimating the distributions of the perimeter of the convex hull, based on the hypothesis that the perimeter of the convex hull of a set of five clustered points is smaller than the one formed by random points. This convex hull is computed based on the points that users select as passwords within an image measuring 1920 × 1080 pixels, using the built-in function convhull in Matlab R2018a relying on the Qhull algorithm. The test was formulated by choosing the optimal distribution that fits the data from a total of 54 distributions, evaluated using the Kolmogorov–Smirnov, Anderson–Darling, and Chi-squared tests, thus achieving the highest reliability. Evaluating the effectiveness of the proposed test involves estimating type I and I I errors, for five levels of significance α ∈ { 0.01 , 0.02 , 0.05 , 0.1 , 0.2 } , by simulating datasets of random and clustered graphical passwords with different levels of clustering. In this study, we compare the effectiveness and efficiency of the proposed test with existing tests from the literature that can detect this type of pattern in Passpoints graphical passwords. Our findings indicate that the new test demonstrates a significant improvement in effectiveness compared to previously published tests. Furthermore, the joint application of the two tests also shows improvement. Depending on the significance level determined by the user or system, the enhancement results in a higher detection rate of clustered passwords, ranging from 0.1 % to 8 % compared to the most effective previous methods. This improvement leads to a decrease in the estimated probability of committing a type I I error. In terms of efficiency, the proposed test outperforms several previous tests; however, it falls short of being the most efficient, using computation time measured in seconds as a metric. It can be concluded that the newly developed test demonstrates the highest effectiveness and the second-highest efficiency level compared to the other tests available in the existing literature for the same purpose. The test was designed to be implemented in graphical authentication systems to prevent users from selecting weak graphical passwords, enhance password strength, and improve system security. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
24. A Multi-Target Pharmacological Correction of a Lipoyltransferase LIPT1 Gene Mutation in Patient-Derived Cellular Models.
- Author
-
Gómez-Fernández, David, Romero-González, Ana, Suárez-Rivero, Juan M., Cilleros-Holgado, Paula, Álvarez-Córdoba, Mónica, Piñero-Pérez, Rocío, Romero-Domínguez, José Manuel, Reche-López, Diana, López-Cabrera, Alejandra, Ibáñez-Mico, Salvador, Castro de Oliveira, Marta, Rodríguez-Sacristán, Andrés, González-Granero, Susana, García-Verdugo, José Manuel, and Sánchez-Alcázar, José A.
- Subjects
INBORN errors of metabolism ,WESTERN immunoblotting ,IRON overload ,BIOENERGETICS ,MUSCLE tone - Abstract
Mutations in the lipoyltransferase 1 (LIPT1) gene are rare inborn errors of metabolism leading to a fatal condition characterized by lipoylation defects of the 2-ketoacid dehydrogenase complexes causing early-onset seizures, psychomotor retardation, abnormal muscle tone, severe lactic acidosis, and increased urine lactate, ketoglutarate, and 2-oxoacid levels. In this article, we characterized the disease pathophysiology using fibroblasts and induced neurons derived from a patient bearing a compound heterozygous mutation in LIPT1. A Western blot analysis revealed a reduced expression of LIPT1 and absent expression of lipoylated pyruvate dehydrogenase E2 (PDH E2) and alpha-ketoglutarate dehydrogenase E2 (α-KGDH E2) subunits. Accordingly, activities of PDH and α-KGDH were markedly reduced, associated with cell bioenergetics failure, iron accumulation, and lipid peroxidation. In addition, using a pharmacological screening, we identified a cocktail of antioxidants and mitochondrial boosting agents consisting of pantothenate, nicotinamide, vitamin E, thiamine, biotin, and α-lipoic acid, which is capable of rescuing LIPT1 pathophysiology, increasing the LIPT1 expression and lipoylation of mitochondrial proteins, improving cell bioenergetics, and eliminating iron overload and lipid peroxidation. Furthermore, our data suggest that the beneficial effect of the treatment is mainly mediated by SIRT3 activation. In conclusion, we have identified a promising therapeutic approach for correcting LIPT1 mutations. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
25. Modulation of the Hyperglycemia Condition in Diabetic Lab Rats with Extracts of the Creole Jamaica Flower (Hibiscus sabdariffa L.) from the Morelia Region (Mexico).
- Author
-
Suárez-Diéguez, Teodoro, Palma-Morales, Marta, Camacho Bernal, Gloria Isabel, Valdez López, Erick Noe, Rodríguez-Pérez, Celia, Cruz-Cansino, Nelly del Socorro, and Nieto, Juan Antonio
- Subjects
LABORATORY rats ,GLYCEMIC control ,ROSELLE ,INSULIN resistance ,BLOOD sugar ,GLYCOSYLATED hemoglobin - Abstract
Extracts from Jamaica flowers (Hibiscus sabdariffa) from Morelia (Mexico) were evaluated as antidiabetic ingredients in a diabetic rat lab model for 80 days at doses of 200, 400, and 600 mg extract/kg rat weight. The hydroalcoholic extract (water:ethanol 80:20 (v/v) at 50 °C) showed a TPC value of 403.28 ± 7.71 mg GAE/g extract, and an antioxidant activity of 0.219 ± 0.00003 mmol Trolox/g (ABTS) and 0.134 ± 0.00001 mmol Trolox/g (DPPH). The extract allowed reducing the diabetic glucose plasma levels under fasting conditions in a dose-dependent manner by 35.2%, 41.63%, and 50.1%. Additionally, the highest dose of the extract (600 mg/kg) slightly reduced the short-term postprandial glucose response while improving the long-term response, reducing hyperglycemia by 45.1%. The same dose also improved lipid metabolism by reducing total cholesterol, triglycerides, VLDL, and LDL, while the HDL level increased. The improvement in glucose and lipid management in the treated groups also led to reduced levels of glycosylated hemoglobin, as well as lower insulin resistance (TyG index), compared to the diabetic control group. The results of this study suggest that extracts from Hibiscus sabdariffa (Morelia) can be used as potential functional ingredients or nutraceuticals for managing the diabetic condition. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
26. Paraoxonase I Activity and Its Relationship with Nutrition in Amyotrophic Lateral Sclerosis.
- Author
-
Proaño, Belén, Benlloch, María, Sancho-Castillo, Sandra, Privado, Jesús, Bargues-Navarro, Guillermo, Sanchis-Sanchis, Claudia Emmanuela, Martínez Bolós, Palmira, Carriquí-Suárez, Ana Belén, Cubero-Plazas, Laura, Platero Armero, Jose Luis, Escriva, Dolores, Ceron, Jose Joaquín, Tvarijonaviciute, Asta, and de la Rubia Ortí, Jose Enrique
- Subjects
AMYOTROPHIC lateral sclerosis ,HEART beat ,HIGH density lipoproteins ,STRUCTURAL equation modeling ,NEURODEGENERATION - Abstract
Background: Amyotrophic lateral sclerosis (ALS) is characterized by progressive motor neuron degeneration, with oxidative stress playing a key role. Paraoxonase 1 (PON1) is an antioxidant enzyme that may influence ALS progression. This study aimed to establish a predictive model for the influence of PON1 activity on functionality in ALS patients and explore its relationship with nutrition. Methods: In this observational cross-sectional study, 70 ALS patients underwent assessments of PON1 activity, lipid profile, functional capacity, respiratory function, and heart rate variability. A structural equation model was developed to determine the relationships between variables. Nutritional intake was analyzed in 65 patients. Results: The predictive model showed that PON1 activity and LDL levels positively influenced functionality, both directly and indirectly through respiratory capacity. Heart rate variability moderately predicted functionality independently. HDL levels were not significantly associated with functionality. Weak to moderate correlations were found between PON1 activity and intake of certain nutrients, with positive associations for monounsaturated fats and vitamin D, and negative associations for carbohydrates, proteins, and some micronutrients. Conclusions: PON1 activity appears to play an important role in ALS patient functionality, both directly and through effects on respiratory capacity. However, its relationship with nutritional intake was not strongly evident in this sample population. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
27. Alzheimer's Disease: Cellular and Pharmacological Aspects.
- Author
-
Aranda-Abreu, Gonzalo Emiliano, Rojas-Durán, Fausto, Hernández-Aguilar, María Elena, Herrera-Covarrubias, Deissy, Chí-Castañeda, Lizbeth Donají, Toledo-Cárdenas, María Rebeca, and Suárez-Medellín, Jorge Manuel
- Published
- 2024
- Full Text
- View/download PDF
28. The Usefulness of Coregistration with iFR in Tandem or Long Diffuse Coronary Lesions: The iLARDI Randomized Clinical Trial.
- Author
-
Hidalgo, Francisco, Gonzalez-Manzanares, Rafael, Suárez de Lezo, Javier, Gallo, Ignacio, Alvarado, Marco, Perea, Jorge, Maestre-Luque, Luis Carlos, Resúa, Adriana, Romero, Miguel, López-Benito, María, Pérez de Prado, Armando, Ojeda, Soledad, and Pan, Manuel
- Subjects
PERCUTANEOUS coronary intervention ,CORONARY artery stenosis ,CORONARY artery disease ,ANGIOGRAPHY ,CONFIDENCE intervals - Abstract
Background. Despite technical advancements, patients with sequential or diffuse coronary lesions undergoing percutaneous coronary intervention (PCI) have an increased risk of cardiovascular events at follow-up. We aimed to analyze the utility of a SyncVision/iFR (S-iFR)-guided PCI strategy versus an angiography-guided strategy in patients with this type of lesions. Methods. Randomized, multicenter, controlled, and open-label trial to compare S-iFR versus angiography-guided PCI in patients with sequential or diffuse angiographic coronary stenosis (ClinicalTrials.gov identifier: NCT04283734). The primary endpoint was the implanted stent length. The main secondary endpoint was targeting vessel failure (TVF) at one year. Results. A total of 100 patients underwent randomization, with 49 patients assigned to the S-iFR group and 51 to the angiography-guided PCI group. There were no differences between groups regarding clinical and anatomical characteristics. The baseline iFR was 0.71 ± 0.16 vs. 0.67 ± 0.19 (p = 0.279) in the S-iFR and angiography group, respectively. The mean lesion length was 42.3 ± 12 mm and 39.8 ± 12 (p = 0.297). The implanted stent length was 32.7 ± 17.2 mm in the S-iFR group and 43.1 ± 14.9 mm in the angiography group (mean difference, −10.4 mm; 95% confidence interval [CI], −16.9 to −4.0; p = 0.002). At one year, target vessel failure (TVF) occurred in four patients: three (6.1%) in the S-iFR group vs. one (1.9%) in the angiography group (p = 0.319). Conclusions. Among patients with sequential or long diffuse coronary lesions, a S-iFR-guided PCI strategy resulted in a reduction of the total stent length compared to an angiography-guided PCI strategy. A nonsignificant increase in TVF was observed in the S-iFR group. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
29. Comparative Efficacy of Neuromodulation and Structured Exercise Program on Autonomic Modulation in Fibromyalgia Patients: Pilot Study.
- Author
-
Rubio-Zarapuz, Alejandro, Apolo-Arenas, María Dolores, Fernandes, Orlando, Tornero-Aguilera, José Francisco, Clemente-Suárez, Vicente J., and Parraca, Jose A.
- Subjects
JOINT pain ,HEART beat ,SLEEP interruptions ,AUTONOMIC nervous system ,FATIGUE (Physiology) - Abstract
Background: Fibromyalgia is a chronic disorder marked by widespread muscle and joint pain, persistent fatigue, sleep disturbances, and irregularities in the autonomic nervous system (ANS). Methods: This study compared the effectiveness of neuromodulation using the EXOPULSE Mollii suit with a structured exercise program in regulating ANS function in fibromyalgia patients. In this randomized, longitudinal crossover study, 10 female patients were randomly assigned to either the Suit + Exercise group or the Exercise + Suit group. Each group participated in two sessions per week for eight weeks, followed by a two-week washout period before switching to the other intervention. We measured cortical arousal, microcirculation, and heart rate variability (HRV) before and after the 1st, 8th, and 16th sessions. Results: The results showed significant improvements in cortical arousal, HRV, and microcirculation with the neuromodulation treatment whereas the exercise program only produced short-term improvements in cortical arousal. Conclusion: The EXOPULSE Mollii suit exhibited cumulative benefits on ANS modulation over time, suggesting potential long-term advantages for managing fibromyalgia. However, further research is needed to explore the delayed effects of both treatments on ANS modulation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
30. Effectiveness of Elastodontic Devices for Correcting Sagittal Malocclusions in Mixed Dentition Patients: A Scoping Review.
- Author
-
Ureni, Rebecca, Verdecchia, Alessio, Suárez-Fernández, Carlota, Mereu, Manuela, Schirru, Roberto, and Spinas, Enrico
- Subjects
MIXED dentition ,ORTHODONTIC appliances ,MALOCCLUSION ,SAMPLE size (Statistics) ,ORTHODONTICS - Abstract
Elastodontics is an interceptive orthodontic therapy that uses light and biological elastic forces through preformed or custom-made removable orthodontic appliances. This study aims to evaluate the effects of elastodontic devices on correcting sagittal discrepancies in growing subjects with mixed dentition. Electronic research was conducted on four databases: PubMed, Scopus, EMBASE, and Web of Science. Data were extracted based on the first author, year of publication, setting and country, study design, sample characteristics, sample size calculation, type of malocclusion, intervention, control group type, compliance, follow-up, and cephalometric measurements. Sixteen studies were included in the final review. Most studies observed a statistically significant reduction (p < 0.05) in SNB and ANB angles. Ten studies reported a reduction in overjet, while eight studies found no change in facial divergence. Comparisons with conventional functional devices revealed no consensus on the skeletal and dentoalveolar effects. Elastodontic appliances significantly improve cephalometric and dentoalveolar parameters, potentially correcting skeletal and dental relationships. However, result variability and unclear advantages over traditional appliances highlight the need for further research. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
31. Physiological, Biomechanical, and Thermographic Responses in Male Athletes during an Ultra-Endurance Race.
- Author
-
Belinchón-deMiguel, Pedro, Ramos-Campo, Domingo Jesús, and Clemente-Suárez, Vicente Javier
- Subjects
HEART beat ,RACE ,BLOOD lactate ,BLOOD sugar ,MALE athletes - Abstract
This study investigates the comprehensive physiological, biomechanical, and thermographic responses of male athletes during an ultra-endurance race, the Santander Four Days (S4D). Involving a 160 km race over four consecutive days with a 10 kg backpack, the study focuses on key aspects such as body mass, cortical arousal, handgrip strength, heart-rate variability, hydration status, blood glucose and lactate concentrations, and thermographic responses. The results indicate changes in heart-rate variability, indicating increased cardiovascular strain, consistent neuromuscular performance, significant body-weight reduction possibly due to dehydration and energy use, stable pH and glucose, but increased protein in urine suggesting renal stress, and varied body temperatures reflecting physical exertion and environmental factors. These findings highlight the body's adaptive mechanisms and the importance of specialized training and recovery strategies in such physically demanding events. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
32. Google Gemini's Performance in Endodontics: A Study on Answer Precision and Reliability.
- Author
-
Díaz-Flores García, Victor, Freire, Yolanda, Tortosa, Marta, Tejedor, Beatriz, Estevez, Roberto, and Suárez, Ana
- Subjects
GEMINI (Chatbot) ,LANGUAGE models ,ARTIFICIAL intelligence ,CHATBOTS ,STATISTICS - Abstract
(1) Background: Large language models (LLMs) are revolutionising various scientific fields by providing advanced support tools. However, the effectiveness of these applications depends on extensive, up-to-date databases to ensure certainty and predictive power. Transparency about information sources in Medicine remains a significant issue. (2) Methods: To evaluate Google Gemini's accuracy and reproducibility in endodontic diagnosis and treatment, 60 questions were designed based on the European Society of Endodontology Position Statements. Thirty questions were randomly selected and answered using Gemini during April 2023. Two endodontic experts independently scored the answers using a 3-point Likert scale. Discrepancies were resolved by a third expert. The relative frequency and absolute percentage of responses were detailed. Accuracy was assessed using the Wald binomial method, and repeatability was assessed using percentage agreement, Brennan and Prediger's coefficient, Conger's generalised kappa, Fleiss' kappa, Gwet's AC, and Krippendorff's alpha, all with 95% confidence intervals. Statistical analysis was performed using STATA software. (3) Results: A total of 900 answers were generated. The percentage of correct answers varied from 0% to 100% per question. Overall accuracy was 37.11% with a 95% confidence interval of 34.02–40.32%; (4) Conclusions: Gemini is not currently designed for medical use and therefore needs to be used with caution when considered for this purpose. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
33. The Influence of Different Solvents on the Physical Properties of ZnO Thin Films.
- Author
-
López-Suárez, Alejandra and Acosta, Dwight R.
- Subjects
THIN films ,ZINC oxide films ,SUBSTRATES (Materials science) ,RUTHERFORD backscattering spectrometry ,OPTICAL spectroscopy ,ULTRAVIOLET spectroscopy ,ETHANOL - Abstract
Polycrystalline zinc oxide (ZnO) thin films were deposited on soda-lime glass substrates using the chemical spray pyrolysis method at three different substrate temperatures: 400, 450, and 500 °C. The solvents used in the precursor solution consisted of either ethanol or methanol. The effects of these solvents on the compositional, structural, morphological, electrical, and optical properties were studied with different techniques, including Rutherford Backscattering Spectrometry (RBS), X-ray diffraction (XRD), Scanning Electron Microscopy (SEM), four-point method, and Ultraviolet and Visible Spectroscopy (Uv-Vis). The results show that both temperature and the type of solvent modify the properties of the materials. An essential outcome of the study was that at 500 °C, the ZnO thin films prepared with either ethanol or methanol exhibited almost the same high-quality crystallinity, stoichiometry, average crystallite size, energy band gap, and resistivity. These findings contribute to our understanding of the properties of these materials and their potential applications. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
34. Gold Nanoparticle Virus-like Particles Presenting SARS-CoV-2 Spike Protein: Synthesis, Biophysical Properties and Immunogenicity in BALB/c Mice.
- Author
-
Salazar, Vivian A., Comenge, Joan, Suárez-López, Rosa, Burger, Judith A., Sanders, Rogier W., Bastús, Neus G., Jaime, Carlos, Joseph-Munne, Joan, and Puntes, Victor
- Subjects
GOLD nanoparticles ,VIRUS-like particles ,COVID-19 vaccines ,ANTIBODY titer ,IMMUNE response - Abstract
Gold nanoparticles (AuNPs) decorated with antigens have recently emerged as promising tools for vaccine development due to their innate ability to provide stability to antigens and modulate immune responses. In this study, we have engineered deactivated virus-like particles (VLPs) by precisely functionalizing gold cores with coronas comprising the full SARS-CoV-2 spike protein (S). Using BALB/c mice as a model, we investigated the immunogenicity of these S-AuNPs-VLPs. Our results demonstrate that S-AuNPs-VLPs consistently enhanced antigen-specific antibody responses compared to the S protein free in solution. This enhancement included higher binding antibody titers, higher neutralizing capacity of antibodies, and stronger T-cell responses. Compared to the mRNA COVID-19 vaccine, where the S protein is synthesized in situ, S-AuNPs-VLPs induced comparable binding and neutralizing antibody responses, but substantially superior T-cell responses. In conclusion, our study highlights the potential of conjugated AuNPs as an effective antigen-delivery system for protein-based vaccines targeting a broad spectrum of infectious diseases and other emergent viruses. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
35. Feasibility, Safety, and Effects of an Aerobic Training Program with Blood Flow Restriction on Functional Capacity, and Symptomatology in Women with Fibromyalgia: A Pilot Study.
- Author
-
Rodríguez-Bautista, José Carlos, López-Lluch, Guillermo, Rodríguez-Torres, Patricia, López-Moral, Álvaro, Quijada-Carrera, Jesús, Bueno-Antequera, Javier, Blanco-Suárez, Manuel, Cáceres-Calle, Óscar, and Munguia-Izquierdo, Diego
- Subjects
BLOOD flow restriction training ,AEROBIC exercises ,FIBROMYALGIA ,FITNESS walking ,REST periods ,PHYSICAL training & conditioning - Abstract
Background: Evidence suggests that aerobic training with blood flow restriction is beneficial for treating fibromyalgia. This study evaluated the feasibility, safety, and effects of an aerobic training program with blood flow restriction for women with fibromyalgia. Methods: Thirty-seven women with fibromyalgia were included, and thirteen with an average age of 59 ± 3, a BMI of 26 ± 3, and who were polymedicated started the intervention period. The intervention group performed aerobic exercise with blood flow restriction using occlusive bands placed in the upper part of the rectus femoris, with a total duration of 14 min of restriction divided into two periods of 7 min with a rest period of 3 min and a total session duration of 17 min. Pressure intensity was measured using the visual pain scale (VAS), scoring 7 out of 10 (n = 7). The non-intervention group performed aerobic exercise without restriction of blood flow for the same periods, rest periods, and total duration of the session (n = 6). The intervention included 2 weekly sessions with 72 h between aerobic walking for 9 weeks. Walking was measured individually using the rating of perceived exertion scale (RPE) with an intensity between 6 and 7 out of 10. Visual and verbal support for the VAS and RPE scale was always provided throughout the sessions supervised by the investigator. Functional capacity was assessed using tests (six-minute walk test, incremental shuttle walk test, knee extension and handgrip test by dynamometer, 30 s chair stand test, and timed up-and-go test). Symptomatology was assessed using questionnaires (Widespread Pain Index, Symptom Severity Score, Fibromyalgia Impact Questionnaire, and Multidimensional Fatigue Inventory), and blood samples were collected. Results: There were no adverse effects, and only one participant in the intervention group withdrew. Between-group and intragroup differences showed that the intervention group obtained improvements in the functional tests; CST p = 0.005; 6MWT p = 0.011; Handgrip p = 0.002; TUGT p = 0.002 with reduced impact of the disease according to the questionnaires; FIQ Stiffness p = 0.027 compared with the nonintervention group. Biochemical results remained within normal ranges in both groups. Conclusions: Blood flow-restricted aerobic training may be feasible, safe, and more effective than unrestricted aerobic training as a physical exercise prescription tool to improve cardiorespiratory fitness, strength, balance, and stiffness in women with fibromyalgia. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
36. Elastoplastic and Electrochemical Characterization of x TiB 2 Strengthened Ti Porous Composites for Their Potential Biomedical Applications.
- Author
-
Bravo-Bárcenas, David Israel, Suárez-Martínez, Reynier, Pérez-Alvarez, Jonatan, Jiménez-Alemán, Omar, Farias-Velazquez, Iván Gerardo, Gonzaléz-Albarrán, Marco Aurelio, and Chávez, Jorge
- Subjects
POROSITY ,NANOINDENTATION tests ,YOUNG'S modulus ,HOLDER spaces ,POROUS materials - Abstract
The microstructure, elastoplastic properties, and corrosive response of induced porous Ti-TiH
2 materials reinforced with TiB2 particles were investigated. Samples were fabricated using CP-Ti Grade1, Titanium Hydride (TiH2 ), TiB2 powders (0, 3, 10, and 30 vol.%), and ammonium bicarbonate salt (40 vol.%) as a space holder. Composites were fabricated using the Powder Metallurgy technique under high-vacuum conditions (HVS) at 1100 °C. Scanning electron microscopy, X-ray diffraction, nanoindentation tests, and electrochemical assays were used to investigate the pore formation, pore distribution, phase formation, elastoplastic properties, and electrochemical behavior of the compounds, respectively. With a mean pore diameter of 50–900 µm and Young's modulus of less than 100 GPa, which is close to the properties of human bone, the pore structures of the compounds processed here are shown to be a potential biomaterial for osseointegration. In addition, their H / E r and H 3 / E r 2 ratios for the reinforced samples are higher than those of the unreinforced sample (1.5 and 4 times higher than the unreinforced sample, respectively), suggesting a better wear resistance of the Ti-TiH2 /xTiB2 composites. Electrochemical experiments demonstrated that the Ti-TiH2 /xTiB2 composites exhibited superior passivation properties compared to the Ti-TiH2 sample. Additionally, the corrosion rates exhibited by the 3 and 10 vol.% of TiB2 samples were found to be within an acceptable range for potential biomedical applications (29.26 and 185.82 E-3 mm·y−1 ). The elastoplastic properties combined with the electrochemical behavior place the Ti-TiH2 /3-10TiB2 composites as potential candidates for the biomedical application of CP-Ti. [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
37. Sesame Detection in Food Using DNA-Functionalized Gold Nanoparticles: A Sensitive, Rapid, and Cost-Effective Colorimetric Approach.
- Author
-
Llano-Suárez, Pablo, Sánchez-Visedo, Adrián, Ortiz-Gómez, Inmaculada, Fernández-Argüelles, María Teresa, Prado, Marta, Costa-Fernández, José Manuel, and Soldado, Ana
- Subjects
SESAME oil ,ANALYSIS of colors ,SUNFLOWER seed oil ,GOLD nanoparticles ,FOOD chemistry - Abstract
Food safety control is a key issue in the food and agriculture industries. For such purposes, developing miniaturized analytical methods is critical for enabling the rapid and sensitive detection of food supplements, allergens, and pollutants. Here, a novel bioanalytical methodology based on DNA-functionalized gold nanoparticles (AuNPs) and colorimetric detection was developed to detect the presence of sesame (a major allergen) through sesame seed DNA as a target, in food samples. The presence of sesame DNA induces controlled nanoparticle aggregation/desegregation, resulting in a color change (from blue to red) proportional to sesame DNA concentration. The incorporation of multicomponent nucleic acid enzymes (MNAzymes) in this strategy has been carried out to perform an isothermal signal amplification strategy to improve the sensitivity of detection. Also, open-source software for color analysis was used to ensure an unbiased visual color-change detection, enhancing detection accuracy and sensitivity and opening the possibility of performing a simple and decentralized analyte detection. The method successfully detected the presence of sesame DNA in sesame seed, sesame oil, olive oil, and sunflower oil. In brief, the developed approach constitutes a simple and affordable alternative to perform a highly sensitive detection of DNA in food without complex methodologies or the requirement of expensive instrumentation. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
38. Sustainable Electrically Conductive Bio-Based Composites via Radical-Induced Cationic Frontal Photopolymerization.
- Author
-
Moraru, Dumitru, Cortés, Alejandro, Martinez-Diaz, David, Prolongo, Silvia G., Jiménez-Suárez, Alberto, and Sangermano, Marco
- Subjects
THERMAL conductivity ,ELECTRIC conductivity ,DYNAMIC mechanical analysis ,CARBON fibers ,WASTE recycling - Abstract
Diglycidylether of vanillyl alcohol (DGEVA), in combination with mechanically recycled carbon fibers (RCFs), was used to make, via Radical-Induced Cationic Frontal Photopolymerization (RICFP), fully sustainable and bio-based conductive composites with good electrical conductivity and consequent Joule effect proprieties. Three different fiber lengths, using three different sieve sizes during the mechanical recycling process (0.2, 0.5, and 2.0 mm), were used in five different amounts (ranging from 1 to 25 phr). The samples were first characterized by dynamic mechanical thermal analysis (DMTA), followed byelectrical conductivity and Joule heating tests. More specifically, the mechanical properties of the composites increased when increasing fiber content. Furthermore, the composites obtained with the longest fibers showed the highest electrical conductivity, reaching a maximum of 11 S/m, due to their higher aspect ratio. In this context, the temperature reached by Joule effect was directly related to the electrical conductivity, and was able to reach an average and maximum temperatures of 80 °C and 120 °C, respectively, just by applying 6 V. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
39. A Formal Synthesis of (+)-Hannokinol Using a Chiral Horner–Wittig Reagent.
- Author
-
Tapera, Michael, Borghi, Federica, Mayer-Figge, Jan Lukas, Mittendorf, Fabia, Celik, Ibrahim-Ethem, Gómez-Suárez, Adrián, and Kirsch, Stefan F.
- Subjects
GLYCOLS - Abstract
Herein, we report a concise and efficient formal synthesis of (+)-hannokinol. Key to this new strategy is the use of a chiral Horner–Wittig reagent, readily available from 2-deoxy-D-ribose, to introduce the chiral 1,3-diol motif. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
40. Impact of the Choice of Diagnostic Criteria and Growth Reference on the Prevalence of Extrauterine Growth Restriction in Extremely-Low-Birthweight Infants.
- Author
-
González López, Clara, Solís Sánchez, Gonzalo, Fernández Colomer, Belén, Mantecón Fernández, Laura, Lareu Vidal, Sonia, Fernández Castiñeira, Sara, Rubio Granda, Ana, Pérez Pérez, Alicia, and Suárez Rodríguez, Marta
- Subjects
CROSS-sectional method ,QUALITATIVE research ,FETAL growth retardation ,SCIENTIFIC observation ,REFERENCE books ,HUMAN growth ,RETROSPECTIVE studies ,QUANTITATIVE research ,DESCRIPTIVE statistics ,LOW birth weight ,LONGITUDINAL method ,MEDICAL records ,ACQUISITION of data ,RESEARCH ,GESTATIONAL age ,STATISTICS ,ANTHROPOMETRY ,DATA analysis software - Abstract
Background and objectives: Variable diagnostic criteria and growth charts have been used for extrauterine growth restriction (EUGR). The objective was to assess the prevalence and concordance of EUGR in extremely-low-birthweight (ELBW) infants with the most frequent diagnostic criteria and growth charts. Materials and methods: An observational, retrospective and multicenter study was conducted from 2011 to 2020 including ELBW infants from the Spanish SEN1500 Network. EUGR prevalence was calculated at discharge using different definitions: cross-sectional (anthropometry less than the 10th centile), longitudinal (decrease of more than 1 SD from birth to discharge), "true" cross-sectional and "true" longitudinal (using the criteria previously described, excluding infants small for gestational age at birth). Concordance among Fenton, Olsen and INTERGROWTH-21st was assessed with Fleiss' Kappa coefficient. Results: The prevalence of EUGR was variable with the different definitions and growth references studied in the 7914 ELBW infants included. Overall, it was higher with Fenton for all the EUGR criteria studied by weight and length. The agreement among growth charts was substantial (κ > 0.6) for all the definitions except for longitudinal EUGR by weight (moderate, κ = 0.578). Conclusions: The prevalence of EUGR was variable in our cohort with the different diagnostic criteria and growth charts. The agreement among charts was good for all the definitions of EUGR except longitudinal EUGR by weight. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
41. Application of Functional Neurology Therapy in a Lactose-Intolerant Patient.
- Author
-
Rey-Mota, Jorge, Escribano-Colmena, Guillermo, Álvarez, David Martín-Caro, Vasquez Perez, Jhulliana, Navarro-Jimenez, Eduardo, and Clemente-Suárez, Vicente Javier
- Subjects
LACTOSE intolerance ,BIOMARKERS ,BREATH tests ,SYMPTOMS ,WOMEN patients - Abstract
This case report examines the impact of a single session of functional neurology on a 35-year-old female patient diagnosed with lactose intolerance. The patient presented with severe gastrointestinal symptoms, including frequent diarrhea, bloating, and vomiting upon dairy consumption. The intervention aimed to reset dysfunctional neurological programs believed to contribute to her condition. The study utilized a standardized lactose intolerance breath test to measure the hydrogen and methane levels at various intervals before and after treatment. Post-treatment results showed symptomatic relief with the patient reporting normalized bowel movements and the absence of previous symptoms. Despite these improvements, the biochemical markers at higher time points (150 and 175 min) post-treatment remained similar to the pre-treatment values, indicating persistent lactose malabsorption and highlighting the variability of hydrogen measurements. This case report suggests that a single session of functional neurology can significantly alleviate the symptoms of lactose intolerance. However, the preliminary nature of these results underscores the need for further research involving larger sample sizes and long-term follow-up to fully understand the treatment's efficacy and underlying mechanisms. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
42. Antibiofilm and Immune-Modulatory Activity of Cannabidiol and Cannabigerol in Oral Environments—In Vitro Study.
- Author
-
Garzón, Hernan Santiago, Loaiza-Oliva, Manuela, Martínez-Pabón, María Cecilia, Puerta-Suárez, Jenniffer, Téllez Corral, Mayra Alexandra, Bueno-Silva, Bruno, Suárez, Daniel R., Díaz-Báez, David, and Suárez, Lina J.
- Subjects
CANNABIDIOL ,PERIODONTAL ligament ,PROPIDIUM iodide ,CYTOTOXINS ,FLOW cytometry ,MARIJUANA ,STREPTOCOCCUS - Abstract
Objective: To evaluate the in vitro antimicrobial and antibiofilm properties and the immune modulatory activity of cannabidiol (CBD) and cannabigerol (CBG) on oral bacteria and periodontal ligament fibroblasts (PLF). Methods: Cytotoxicity was assessed by propidium iodide flow cytometry on fibroblasts derived from the periodontal ligament. The minimum inhibitory concentration (MIC) of CBD and CBG for S. mutans and C. albicans and the metabolic activity of a subgingival 33-species biofilm under CBD and CBG treatments were determined. The Quantification of cytokines was performed using the LEGENDplex kit (BioLegend, Ref 740930, San Diego, CA, USA). Results: CBD-treated cell viability was greater than 95%, and for CBG, it was higher than 88%. MIC for S. mutans with CBD was 20 µM, and 10 µM for CBG. For C. albicans, no inhibitory effect was observed. Multispecies biofilm metabolic activity was reduced by 50.38% with CBD at 125 µg/mL (p = 0.03) and 39.9% with CBG at 62 µg/mL (p = 0.023). CBD exposure at 500 µg/mL reduced the metabolic activity of the formed biofilm by 15.41%, but CBG did not have an effect. CBG at 10 µM caused considerable production of anti-inflammatory mediators such as TGF-β and IL-4 at 12 h. CBD at 10 µM to 20 µM produced the highest amount of IFN-γ. Conclusion: Both CBG and CBD inhibit S. mutans; they also moderately lower the metabolic activity of multispecies biofilms that form; however, CBD had an effect on biofilms that had already developed. This, together with the production of anti-inflammatory mediators and the maintenance of the viability of mammalian cells from the oral cavity, make these substances promising for clinical use and should be taken into account for future studies. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
43. Phenotypic Heterogeneity of Cancer Associated Fibroblasts in Cervical Cancer Progression: FAP as a Central Activation Marker.
- Author
-
Bueno-Urquiza, Lesly Jazmin, Godínez-Rubí, Marisol, Villegas-Pineda, Julio César, Vega-Magaña, Alejandra Natali, Jave-Suárez, Luis Felipe, Puebla-Mora, Ana Graciela, Aguirre-Sandoval, Gloria Estefanía, Martínez-Silva, María Guadalupe, Ramírez-de-Arellano, Adrián, and Pereira-Suárez, Ana Laura
- Subjects
CERVICAL cancer ,CANCER invasiveness ,FIBROBLASTS ,MESENCHYMAL stem cells ,PHENOTYPES - Abstract
Cervical cancer (CC) is the fourth leading cancer among women and is one of the principal gynecological malignancies. In the tumor microenvironment, cancer-associated fibroblasts (CAFs) play a crucial role during malignant progression, exhibiting a variety of heterogeneous phenotypes. CAFs express phenotypic markers like fibroblast activation protein (FAP), vimentin, S100A4, α-smooth muscle actin (αSMA), and functional markers such as MMP9. This study aimed to evaluate the protein expression of vimentin, S100A4, αSMA, FAP, and MMP9 in mesenchymal stem cells (MSC)-CAF cells, as well as in cervical cancer samples. MSC cells were stimulated with HeLa and SiHa tumor cell supernatants, followed by protein evaluation and cytokine profile to confirm differentiation towards a CAF phenotype. In addition, automated immunohistochemistry (IHQa) was performed to evaluate the expression of these proteins in CC samples at different stages. Our findings revealed a high expression of FAP in stimulated MSC cells, accompanied by the secretion of pro/anti-inflammatory cytokines. In the other hand, CC samples were observed to have high expression of FAP, vimentin, αSMA, and MMP9. Most importantly, there was a high expression of their activation proteins αSMA and FAP during the different stages. In the early stages, a myofibroblast-like phenotype (CAFs αSMA+ FAP+), and in the late stages a protumoral phenotype (CAF αSMA− FAP+). In summary, FAP has a crucial role in the activation of CAFs during cervical cancer progression. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
44. Data Augmentation Effects on Highly Imbalanced EEG Datasets for Automatic Detection of Photoparoxysmal Responses
- Author
-
Moncada Martins, Fernando, González Suárez, Víctor Manuel, Villar Flecha, José Ramón, García López, Beatriz, Moncada Martins, Fernando, González Suárez, Víctor Manuel, Villar Flecha, José Ramón, and García López, Beatriz
- Abstract
Photosensitivity is a neurological disorder in which a person’s brain produces epileptic discharges, known as Photoparoxysmal Responses (PPRs), when it receives certain visual stimuli. The current standardized diagnosis process used in hospitals consists of submitting the subject to the Intermittent Photic Stimulation process and attempting to trigger these phenomena. The brain activity is measured by an Electroencephalogram (EEG), and the clinical specialists manually look for the PPRs that were provoked during the session. Due to the nature of this disorder, long EEG recordings may contain very few PPR segments, meaning that a highly imbalanced dataset is available. To tackle this problem, this research focused on applying Data Augmentation (DA) to create synthetic PPR segments from the real ones, improving the balance of the dataset and, thus, the global performance of the Machine Learning techniques applied for automatic PPR detection. K-Nearest Neighbors and a One-Hidden-Dense-Layer Neural Network were employed to evaluate the performance of this DA stage. The results showed that DA is able to improve the models, making them more robust and more able to generalize. A comparison with the results obtained from a previous experiment also showed a performance improvement of around 20% for the Accuracy and Specificity measurements without Sensitivity suffering any losses. This project is currently being carried out with subjects at Burgos University Hospital, Spain., This research was funded by the Spanish Ministry of Economics and Industry, Grant PID2020-112726RB-I00, by the Spanish Research Agency (AEI, Spain) under Grant agreement RED2018-102312-T (IA-Biomed), and by the Ministry of Science and Innovation under CERVERA Excellence Network project CER-20211003 (IBERUS) and Missions Science and Innovation project MIG-20211008 (INMERBOT), as well as by Principado de Asturias, Grant SV-PA-21-AYUD/2021/50994.
- Published
- 2024
45. Serological Antibodies against Kidney, Liver, and Spleen Membrane Antigens as Potential Biomarkers in Patients with Immune Disorders
- Author
-
Inmunología, microbiología y parasitología, Farmacología, Immunologia, mikrobiologia eta parasitologia, Farmakologia, Hernández Suárez, Leidi, Díez Martín, Eguzkiñe, Egiguren Ortiz, June, Fernández, Roberto, Etxebarria Gallego, Aitor, Astigarraga Arribas, Egoitz, Miguélez Palomo, Cristina, Ramírez Garcia, Andoni, Barreda Gómez, Gabriel, Inmunología, microbiología y parasitología, Farmacología, Immunologia, mikrobiologia eta parasitologia, Farmakologia, Hernández Suárez, Leidi, Díez Martín, Eguzkiñe, Egiguren Ortiz, June, Fernández, Roberto, Etxebarria Gallego, Aitor, Astigarraga Arribas, Egoitz, Miguélez Palomo, Cristina, Ramírez Garcia, Andoni, and Barreda Gómez, Gabriel
- Abstract
Immune disorders arise from complex genetic and environmental factors, which lead to dysregulation at the cellular and inflammatory levels and cause tissue damage. Recent research highlights the crucial role of reactive antibodies in autoimmune diseases and graft rejection, but their complex determination poses challenges for clinical use. Therefore, our study aimed to ascertain whether the presence of reactive antibodies against membrane antigens in tissues from both animal models and humans could serve as biomarkers in patients with autoimmune disorders. To address this issue, we examined the binding profile of serological antibodies against a diverse panel of cell membranes from the spleen, liver, and kidney tissues of monkeys, rats, and humans. After developing the cell membrane microarrays, human sera were immunologically assayed. The study was first conducted on sera from two groups, healthy subjects and patients with inflammatory and autoimmune disorders, and then optimized for kidney transplant patient sera. A significant increase in antibody reactivity against specific monkey kidney and spleen membranes was observed in the serum of patients with lupus nephritis, while kidney transplant patients showed a significant enhancement against human tissues and human embryonic kidney 293 cells. These results show the potential importance for clinical and basic research purposes of studying the presence of specific IgG against membrane antigens in patients’ serum as potential biomarkers of immune disorders. However, it is important to note that these results need to be verified in further studies with a larger sample size to confirm their relevance.
- Published
- 2024
46. Lower Urinary Tract Symptoms (LUTS) as a New Clinical Presentation of Histamine Intolerance: A Prevalence Study of Genetic Diamine Oxidase Deficiency
- Author
-
Ponce Díaz-Reixa, José, Aller Rodríguez, Marcos, Martínez Breijo, Sara, Suanzes Hernández, Jorge, Ruiz Casares, Eva, Perucho Alcalde, Teresa, Bohorquez Cruz, Manuel, Mosquera Seoane, Teresa, Sánchez Merino, Jose M., Freire Calvo, Jacobo, Fernández Suárez, Paula, Chantada Abal, Venancio, Ponce Díaz-Reixa, José, Aller Rodríguez, Marcos, Martínez Breijo, Sara, Suanzes Hernández, Jorge, Ruiz Casares, Eva, Perucho Alcalde, Teresa, Bohorquez Cruz, Manuel, Mosquera Seoane, Teresa, Sánchez Merino, Jose M., Freire Calvo, Jacobo, Fernández Suárez, Paula, and Chantada Abal, Venancio
- Abstract
This work received external funding from Healthcare-AB Biotek HNH. The authors declare that no other funds, grants, or other forms of support were received during the preparation of this manuscript., Lower urinary tract symptoms (LUTS) are highly prevalent, and their treatment is mainly focused on the control of symptoms. Histamine intolerance (HIT) has been related to a variety of systemic symptoms. DAO deficiency has been identified as a significant factor contributing to histamine intolerance (HIT). Preclinical evidence indicates the involvement of histamine in the lower urinary tract. This study aimed to assess the prevalence of diamine oxidase deficiency (DAO) in a prospective cohort of 100 patients with at least moderate LUTS. A genetic study of four single nucleotide polymorphisms (SNPs) (c.-691G>T, c.47C>T, c.995C>T, and c.1990C>G) was performed. HIT was found in 85.9% of patients. The prevalence of at least one minor allele in the SNPs analyzed was 88%, without gender differences. Storage symptoms were more intense in the presence of HIT as well as asthenia and neurological and musculoskeletal symptoms. The presence of minor alleles of the AOC1 gene was associated with a higher intensity of symptoms. Minor alleles from c.-691G>T and c.47C>T SNPs were also associated with a greater severity of obstructive symptoms. Thirty-one percent of patients presented the four SNPS with at least one associated minor allele. The relationship between HIT and LUTS in a mixed population of men and women found in this study supports further investigations to define the pathophysiology of histamine in LUTS., Los síntomas del tracto urinario inferior (STUI) son muy prevalentes y su tratamiento se centra principalmente en el control de los síntomas. La intolerancia a la histamina (HIT) se ha relacionado con diversos síntomas sistémicos. La deficiencia de DAO se ha identificado como un factor importante que contribuye a la intolerancia a la histamina (HIT). Las pruebas preclínicas indican la implicación de la histamina en el tracto urinario inferior. tracto urinario inferior. El objetivo de este estudio era evaluar la prevalencia del déficit de diaminooxidasa (DAO) en una cohorte prospectiva de 100 pacientes con STUI al menos moderados. Se realizó un estudio genético de cuatro polimorfismos de nucleótido único (SNP) (c.-691G>T, c.47C>T, c.995C>T y c.1990C>G). Se detectó TIH en el 85,9% de los pacientes. La prevalencia de al menos un alelo menor en los SNP analizados fue del 88%, sin diferencias de género. Los síntomas de almacenamiento fueron más intensos en presencia de HIT, así como así como la astenia y los síntomas neurológicos y musculoesqueléticos. La presencia de alelos menores del del gen AOC1 se asoció a una mayor intensidad de los síntomas. Los alelos menores de c.-691G>T y c.47C>T SNPs también se asociaron con una mayor intensidad de los síntomas obstructivos. Treinta y uno por ciento de los pacientes presentaban los cuatro SNPS con al menos un alelo menor asociado. La relación entre HIT y STUI en una población mixta de hombres y mujeres hallada en este estudio respalda para definir la fisiopatología de la histamina en los STUI., Depto. de Genética, Fisiología y Microbiología, Fac. de Ciencias Biológicas, TRUE, pub
- Published
- 2024
47. Analysis of the Radiological Changes of the Sinus Membrane Using Cone Beam Computed Tomography and Its Relationship with Dental Treatments. A Retrospective Study
- Author
-
Rey-Martínez, María Helena, Ruiz-Sáenz, Pedro Luis, Martínez Rodríguez, Natalia, Barona Dorado, Cristina, Meniz García, Cristina María, Cortés Bretón Brinkmann, Jorge, Suárez-Quintanilla, Juan Antonio, Martínez González, José María, Rey-Martínez, María Helena, Ruiz-Sáenz, Pedro Luis, Martínez Rodríguez, Natalia, Barona Dorado, Cristina, Meniz García, Cristina María, Cortés Bretón Brinkmann, Jorge, Suárez-Quintanilla, Juan Antonio, and Martínez González, José María
- Abstract
The aim of this study was to identify the most relevant dental factors and iatrogenic causes in the development of pathological changes to the sinus membrane and to analyse their possible influence on the development of odontogenic sinusitis. A descriptive, observational study was designed, with 276 patients who had been evaluated via cone beam computed tomography, analysing possible sinus thickening factors, such as apical infections, endodontic treatments, periodontitis, radicular cysts and impacted teeth, as well as iatrogenic factors caused by implant treatments or the development of oroantral communications produced during tooth extraction manoeuvres. Among the dental factors, periodontitis (47.1%), apical pathology (23.5%) and endodontic treatments (23.1%) were the predominant causes of sinus membrane thickening that most frequently produced an occupancy between 2 and 10 mm. Regarding the implant treatments, the placement of implants through the floor of the maxillary sinus was the main cause (9.8%), followed by sinus elevation techniques (6.2%). Dental extraction was the first cause of oroantral communication (5.0%), being the procedure that caused the greatest thickening of the sinus membrane. This study highlights the importance of dental treatments and iatrogenic factors in sinus pathology, and the need for diagnostic interrelations between the different specialists who address this pathology., Depto. de Especialidades Clínicas Odontológicas, Fac. de Odontología, TRUE, pub
- Published
- 2024
48. Long-term clinical behavior and complications of intentionally tilted dental implants compared with straight implants supporting fixed restorations: A systematic review and metaanalysis
- Author
-
Cortés Bretón Brinkmann, Jorge, García-Gil, Ignacio, Pedregal Patricia, Peláez Rico, Jesús, Prados Frutos, Juan Carlos, Suárez García, María Jesús, Cortés Bretón Brinkmann, Jorge, García-Gil, Ignacio, Pedregal Patricia, Peláez Rico, Jesús, Prados Frutos, Juan Carlos, and Suárez García, María Jesús
- Abstract
Simple Summary: Following tooth loosening due to periodontal disease, caries, trauma, or tumoral processes, bone resorption and remodeling of the alveolar ridge makes the insertion of implants difficult. A number of bone augmentation techniques are available to treat atrophic jaws. However, when posterior bone is lacking and extensive bone augmentation surgeries are rejected by the patient, placing distally tilted posterior implants may offer a valid therapeutic option for implant-supported restorations. This treatment modality places the implants in preexisting bone, improving bone anchorage and prosthetic support. Nevertheless, some studies suggest that for tilted implants, bending moments are greater at the level of the angled abutment. Abstract: The aim of this study was to assess the long-term clinical behavior of straight implants in comparison with intentionally tilted dental implants (ITDI) supporting fixed restorations in partial or total edentulous arches, analyzing implant survival and success rates, complications, and marginal bone loss (MBL) after >5 years of function. An electronic search was conducted in five electronic databases (MEDLINE/Pubmed, Embase, Web of Science, Scopus, and Cochrane Central Register of Controlled Trials) supplemented by a manual search. The electronic and manual search identified 1853 articles, of which 8 articles were selected for analysis. Out of a total of 3987 dental implants, 2036 were axial dental implants and 1951 tilted. Similar results were found in implant survival or overall implant success rates. Moreover, no statistically significant differences were found in MBL (p = 0.369; MD 0.116 mm (−0.137; 0.369) 95% CI) The prosthodontic/biological complications reported in the articles were very diverse and irregularly distributed. This systematic review suggests that there is no difference between tilted compared with straight dental implants in the medium-long term (>5 years). However, further research is needed to gener, Depto. de Odontología Conservadora y Prótesis, Fac. de Odontología, TRUE, pub
- Published
- 2024
49. Effects of Combinations of Dietary Vitamin C and Acetylsalicylic Acid on Growth Performance, Carcass Traits and, Serum and Immune Response Parameters in Broilers
- Author
-
Ferronato, Giulia, Tavakoli, M., Bouyeh, M., Seidavi, A., Suárez Ramírez, L., Prandini, Aldo, Ferronato G., Prandini A. (ORCID:0000-0002-8650-8766), Ferronato, Giulia, Tavakoli, M., Bouyeh, M., Seidavi, A., Suárez Ramírez, L., Prandini, Aldo, Ferronato G., and Prandini A. (ORCID:0000-0002-8650-8766)
- Abstract
This study aimed to investigate the combined effects of ascorbic acid (VC) and acetylsalicylic acid (ASA) on broiler health and performance. A total of 200 Ross 308 male broilers were divided into five groups, each receiving different dietary combinations of ASA and VC (ASA: 50 or 100 mg/kg; VC: 200 or 400 mg/kg). The 42-day trial assessed parameters such as feed intake, average daily gain (ADG), feed conversion ratio (FCR), carcass characteristics, serum parameters, immune response and cecal microbial flora. The results indicate significant treatment effects on feed intake and growth performance, with a higher feed intake of ADG and FCR in treatment groups (p < 0.05). Serum lipid parameters were unaffected, but creatine kinase increased with ASA and VC intake (p < 0.05). Changes in sheep red blood cell titers and influenza antibodies were noted (p < 0.05). The combination of ASA and VC positively influenced carcass traits, reducing abdominal fat and altering the ratio of immune response organs to body weight (p < 0.05). Additionally, the cecal E. coli count decreased with treatment (p < 0.05). This study underscores the intricate interactions between ASA and VC supplementation, growth performance and carcass composition and immune response in broilers. Further research is warranted to explore dosage nuances and variations under specific stress conditions.
- Published
- 2024
50. Comparative Analysis of Meteorological versus In Situ Variables in Ship Thermal Simulations
- Author
-
Arce Fariña, Elena, Suárez-García, Andrés, López-Vázquez, José-Antonio, Devesa-Rey, Rosa, Arce Fariña, Elena, Suárez-García, Andrés, López-Vázquez, José-Antonio, and Devesa-Rey, Rosa
- Abstract
[Abstract] Thermal simulations have become increasingly popular in assessing energy efficiency and predicting thermal behaviors in various structures. Calibration of these simulations is essential for accurate predictions. A crucial aspect of this calibration involves investigating the influence of meteorological variables. This study aims to explore the impact of meteorological variables on thermal simulations, particularly focusing on ships. Using TRNSYS (TRaNsient System Simulation) software (v17), renowned for its capability to model complex energy systems within buildings, the significance of incorporating meteorological data into thermal simulations was analyzed. The investigation centered on a patrol vessel stationed in a port in Galicia, northwest Spain. To ensure accuracy, we not only utilized the vessel’s dimensions but also conducted in situ temperature measurements onboard. Furthermore, a dedicated weather station was installed to capture real-time meteorological data. Data from multiple sources, including Meteonorm and MeteoGalicia, were collected for comparative analysis. By juxtaposing simulations based on meteorological variables against those relying solely on in situ measurements, we sought to discern the relative merits of each approach in enhancing the fidelity of thermal simulations.
- Published
- 2024
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.