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Carotenoids are an abundant group of lipid-soluble antioxidants in maize kernels. Maize is a key target crop for carotenoid biofortification focused on using conventional plant breeding in native germplasm of temperate areas traced back partially to traditional cultivars (landraces). In this study, the objectives were to determine the variability of lutein (LUT), zeaxanthin (ZEA), α-cryptoxanthin (αCX), β-cryptoxanthin (βCX), α-carotene (αC), and β-carotene (βC) contents in the grain of 88 accessions of temperate maize from the Croatian genebank, and to evaluate the relationships among the contents of different carotenoids as well as the relationships between kernel color and hardness and carotenoid content. Highly significant variability among the 88 accessions was detected for all carotenoids. On average, the most abundant carotenoid was LUT with 13.2 μg g−1 followed by ZEA with 6.8 μg g−1 dry matter. A Principal Component Analysis revealed a clear distinction between α- (LUT, αCX, and αC) and β-branch (ZEA; βCX, and βC) carotenoids. β-branch carotenoids were positively correlated with kernel color, and weakly positively associated with kernel hardness. Our results suggest that some genebank accessions with a certain percentage of native germplasm may be a good source of carotenoid biofortification in Southeast Europe. However, due to the lack of association between LUT and ZEA, the breeding process could be cumbersome. [ABSTRACT FROM AUTHOR]

Southeast Europe is considered one of the most vulnerable regions in the context of climate change, and projected future summer warming is expected to exceed global rates significantly. Despite the importance of this problem, there have been few studies that utilized Coupled Model Intercomparison Project Phase 5 (CMIP5) Global Circulation Models (GCMs) and the multi-model ensemble approach to examine extreme heat events on a regional scale. Additionally, the NEX-GDDP dataset, successfully applied in other parts of the world to assess extreme heat, has not yet been utilized for Southeast Europe. This study aims to fill that gap, presenting the time evolution and spatial distribution of extreme heat events in Southeast Europe for the historical period 1950–2005 and for the expected future climate up to the end of the 21st century based on the NEX-GDDP dataset. In order to leverage the strengths of the multi-model ensemble approach, a set of purpose-tailored indicators, such as the annual number of hot days, the maximum number of consecutive hot days, and hot spell duration at different thresholds, is computed by the daily maximum temperature data from all datasets, produced by NEX-GDDP (21 for the historical period and 42 for the future period). The E-OBS dataset is used as a reference for evaluating the NEX-GDDP's capability to simulate the features of the observed historical extreme heat events. The results show that the multi-model ensemble can satisfactorily capture the occurrence of extreme heat events in the historical period, and therefore it is reasonable to assume that the NEX-GDDP dataset has the potential to reproduce such extremes in the projected future. The study provides clear evidence that the persistence and spatial extent of extreme heat will increase significantly. Some indicators that were not relevant for the historical period due to the high-temperature threshold will become helpful in assessing extreme heat in Southeast Europe in the latter part of the century. Thus, under the RCP8.5 scenario, the area-averaged duration of hot spells at 32 °C and 34 °C will increase from near zero in 1976–2005 to 60 and 45 days, respectively, by the end of the century. The indicators used in the study may be helpful for decision-makers to implement climate change mitigation strategies and actions adequately. The findings are consistent with general tendencies in maximum temperatures considered in our previous works but also with the outcomes of recent studies dedicated to the future climate of the region. [ABSTRACT FROM AUTHOR]

Selenium (Se) is a nonmetal that is essential for humans and other animals, and is considered beneficial for plants. The bioavailability of Se strongly influences its content in the food chain. Soils are the main source of Se, and their Se content primarily influences its availability, along with other soil properties. A field survey was conducted on soils of Southeast Europe, specifically in Croatia (Osijek), Bosnia and Herzegovina (Sarajevo, Banja Luka, Mostar, and Prud), and Serbia (Novi Sad). Soil samples were taken from the arable soil layer (0–30 cm depth), and two types of Se availability were measured: Se extracted using pure HNO3 (SeTot) and Se readily extracted in water (SeH2O). Only soils from the Mostar area had Se concentrations above deficit levels (0.5 mg kg−1), with the highest values of cation exchange capacity (CEC), soil organic matter (SOM) measured as loss of ignition (LOI), total C, total N, ZnTot and CdTot. The connections between the chemical characteristics of the soil and SeH2O were investigated. Principal component analysis (PCA) explained 73.7% of the variance in the data set in the first three principal components (PCs). Using the provided data, we developed a partial least squares (PLS) regression model that predicted the amount of SeH2O in the soil, with an accuracy ranging from 77% to 90%, depending on the input data. The highest loadings in the model were observed for LOI, CEC, total C, total N, and SeTot. Our results indicate the need for biofortification in these key agricultural areas to supplement the essential dietary requirements of humans and livestock. To efficiently and economically implement biofortification measures, we recommend utilizing regression models to accurately predict the availability of Se. [ABSTRACT FROM AUTHOR]

The beneficial relationship of entrepreneurial orientation with performance has been extensively acknowledged in the literature. However, empirical evidence is not straightforward and requires consideration of the interplay of different variables, context, and rationales through which this causal complexity of the performance-enhancing mechanism of entrepreneurial orientation influences firm performance. In this paper we examine the way the entrepreneurial behavior of the organization unfolds and affects performance through specific configurations resulting from the simultaneous interaction of strategic networking and dynamic environment in the setting of Southeast European SMEs. We apply hierarchical regression to a sample of 963 small and medium-sized enterprises. Empirical findings provide support for applying the configurational approach and show that companies of high entrepreneurial orientation and high strategic networking operating in a dynamic environment achieve the highest effect on a business performance. Research results further indicate that strategic networking is a mechanism that achieves the greatest efficiency in a dynamic environment and with the presence of a firm's entrepreneurial orientation. According to the research results, implications for research and practice are suggested. [ABSTRACT FROM AUTHOR]

This study presents the results of a landslide susceptibility analysis applied to the Vrancea-Buzău seismogenic region in the Carpathian Mountains, Romania. The target area is affected by a large diversity of landslide processes. Slopes are made-up of various types of rocks, climatic conditions can be classified as wet, and the area is a seismically active one. All this contributes to the observed high landslide hazard. The paper analyses the spatial component of the landslide hazard affecting the target area, the regional landslide susceptibility. First, an existing landslide inventory was completed to cover a wider area for the landslide susceptibility analysis. Second, two types of methods are applied, a purely statistical technique, based on correlations between landslide occurrence and local conditions, as well as the simplified spatial process-based Newmark Displacement analysis. Landslide susceptibility maps have been produced by applying both methods, the second one also allowing us to simulate different scenarios, based on various soil saturation rates and seismic inputs. Furthermore, landslide susceptibility was computed both for the landslide source and runout zones—the first providing information about areas where landslides are preferentially triggered and the second indicating where landslides preferentially move along the slope and accumulate. The analysis showed that any of the different methods applied produces reliable maps of landslide susceptibility. However, uncertainties were also outlined as validation is insufficient, especially in the northern area, where only a few landslides could be mapped due to the intense vegetation cover. [ABSTRACT FROM AUTHOR]

Research on a specific topic requires the individualized cartographic methods of work that may be defined as the Realm of Maps. The double dimensionality in the Realm of Maps is understood here as a physical place—a studio workroom—and as a research method. In this study, we focused on the way of presenting a research method designed to study the topic of historico-geographical space in the form of a short film story. The second purpose is to indicate the legitimacy of combining two dimensions of working with maps, the real one and the virtual one, to be able to collect cartographic and descriptive sources in one scientific center. Our research on the Story from the Realm of Maps in Regensburg: 'People Movement in Southeast Europe' included a concept adopted by cartographical, historical, and geographical sources; the construction of a studio workroom; a script draft; individual sequences of the story in different types of media; editing the video, along with publishing it on an online video-sharing platform. We used as many different types of geomedia as possible, which, on the one hand, boosts the attractiveness of the film and, on the other, may hamper the proper perception of the main film plot. Finally, we recommend principles of map design for the film, with analog maps and maps created specifically for the short film, published using online video-sharing platforms. [ABSTRACT FROM AUTHOR]

Under the EU Water Framework Directive, ecological assessment and management are based on type-specific reference conditions. In the EU it may be difficult to find sites in large rivers with at least near-natural conditions, though this is not the case in southeast Europe, where stretches of large rivers still exist with at least near-natural conditions, meaning that there is little or no disturbance from hydromorphological alteration, water quality, land use in the catchment and alien species. We examined benthic invertebrate assemblages in 45 samples collected from near-natural sites of several large rivers: Sava, Drava, Mura, Kupa and Una. The near-natural benthic invertebrate assemblages of large rivers contained several rare or remarkable species, especially among stoneflies, e.g., Marthamea vitripennis, Xanthoperla apicalis. We compared benthic invertebrate communities in river sections with fine and coarse substrates and in three eco-hydromorphological (ECO-HM) types of large rivers, reflecting habitat heterogeneity: lowland-deep, lowland-braided and intermountain. Multivariate analysis of variance (PERMANOVA) was used to statistically evaluate similarities among assemblages. It was found that the composition of benthic invertebrate assemblages varied by both ECO-HM types and substrate category. Similarity percentage (SIMPER) analysis showed that the average dissimilarity of benthic invertebrate assemblages was high between all ECO-HM type pairs and between fine and coarse substrate. We found that habitat heterogeneity and substrate independently influenced benthic invertebrate assemblages. To achieve ecological goals in the management of large rivers, in addition to functionality, a holistic view with at least near-natural assemblages, including the names of the taxa present, should also be considered. [ABSTRACT FROM AUTHOR]

Combining experimental studies on grain yield variability with crop model simulations in maize could assist in choosing the optimum maturity group for a certain location, counteracting the effect of climate change. However, studies considering specificities in Southeast Europe are lacking. The objectives were to put various environmental covariates including stress degree days (SDD) into FAO maturity settings to determine the impact of climate change on maize growing in Southeast Europe and to compare trends for grain yields over twenty years of maize experimental and simulation data grouped in five FAO maturity groups (FAO 200–FAO 600). Pre-registration yield trials of maize planted in one location in Croatia grown from 1996 to 2015 were used to determine "potential yield". Correlation coefficients between 12 climate covariates and grain yield (GY) across the maturity groups revealed the tightest negative associations between SDD and GY that were weakened by later-maturity groups. Similar trends in GY were obtained by both experimental and simulation data, highlighting FAO 600 as a nearly no yield-reducing FAO group over the two decades. Our results indicate that choosing early maize hybrids in Southeast Europe does not seem to be an optimum option in the future, since these hybrids are more sensitive to omnipresent heat stress than late hybrids. [ABSTRACT FROM AUTHOR]

The objective of this paper is to provide an overview of the current status of rabies in Europe, with special emphasis on Croatia and Southeast and East Europe. Due to the systematic implementation of a rabies eradication program by oral vaccination of wild animals, by the end of the 20th century, most West and Central European countries were rabies-free. The EU goal was to eradicate rabies in wildlife and domestic animals by 2020. No matter how achievable the goal seemed to be, the disease is still present in the eastern part of the EU, as was notified in 2020 by two member states—Poland and Romania. Croatia has been rabies-free for the last seven years but given that it borders a non-EU country in which a case of rabies was confirmed in 2020, it will continue to contribute to the maintenance of the rabies-free region. A rabies-free EU can only be achieved by continuous oral vaccination, coordination and a regional approach. The prevention of reintroductions from bordering countries in which rabies has not been eradicated yet, and the support for the eradication efforts made by these countries, are goals still pending. [ABSTRACT FROM AUTHOR]

This study aimed to assess post-harvest contamination with mycotoxins in the context of the geographic and agroclimatic conditions in Romania in 2012–2015, a period that was characterized by extreme meteorological events and the effects of climate change. The samples were randomly sampled from five agricultural regions of Romania and analyzed for mycotoxins by enzyme-linked immunosorbent assay. An SPSS analysis was done to explore correlations between mycotoxins (deoxynivalenol—DON, aflatoxins—AF, ochratoxin A—OTA, zearalenone—ZEA), product types (raw cereal, processed cereal, cereal-based food), geographic coordinates (latitude, longitude, agricultural region), and agroclimatic factors (air temperature, precipitation, soil moisture reserve, aridity index, soil type). In the southeast part of the Southern Plain and Dobrogea (Baragan Plain, located at 44–45° N, 26–27° E), contamination with AF and OTA was higher in raw and processed cereals (maize in silo, silo corn germs) in the dry years (2012 and 2013), and contamination with DON was high in processed cereals (wheat flour type 450) in the rainy year (2014). DON and OTA contamination were significantly correlated with cumulative precipitation in all years, while AF and ZEA contamination were non-significantly correlated with climatic factors and aridity indices. The distribution of mycotoxins by product type and the non-robust correlations between post-harvest mycotoxins and agrometeorological factors could be explained by the use of quality management systems that control cereal at warehouse receptions, performant processing technologies, and the quality of storage spaces of agri-food companies. The Baragan Plain is Romania's most sensitive area to the predicted climate change in southeast Europe, which may be associated with its increased cereal contamination with AF and OTA. [ABSTRACT FROM AUTHOR]

We investigated whether immigrants from Southeast Europe (SE) and Swiss patients have different reasons for visiting the emergency department (ED). Our retrospective data analysis for the years 2013⁻2017 describes the pattern of ED consultations for immigrants from SE living in Switzerland (Canton Bern), in comparison with Swiss nationals, with a focus on type of referral and reason for admission. A total of 153,320 Swiss citizens and 12,852 immigrants from SE were included in the study. The mean age was 51.30 (SD = 21.13) years for the Swiss patients and 39.70 (SD = 15.87) years for the SE patients. For some countries of origin (Albania, Bosnia and Herzegovina, and Turkey), there were highly statistically significant differences in sex distribution, with a predominance of males. SE immigrants had a greater proportion of patients in the lower triage level (level 3: SE: 67.3% vs. Swiss: 56.0%) and a greater proportion of patients in the high triage level than the Swiss population (level 1: SE: 3.4% vs. Swiss: 8.8%). SE patients of working age (16⁻65 years) were six times more often admitted by ambulance than older (≥65 years) SE patients, whereas this ratio was similar in the Swiss population. In both groups, the fast track service was primarily used for patients of working age (<65) and more than three times more often in the SE than the Swiss group (SE: 39.1%, Swiss: 12.6%). We identified some indications for access to primary care in emergency departments for immigrants and highlighted the need for attention to the role of organizational characteristics of primary health care in Switzerland. We highlighted the need for professional support to improve the quality of healthcare for immigrants. In the future, we will need more primary care services and general practitioners with a migrant background.