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Your search keyword '"Single-channel recording"' showing total 3 results
Start Over Descriptor "Single-channel recording" Publisher mdpi
3 results on '"Single-channel recording"'

1. A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings

Author

Hirano, Minako, Yamamoto, Daiki, Asakura, Mami, Hayakawa, Tohru, Mise, Shintaro, Matsumoto, Akinobu, Ide, Toru, Hirano, Minako, Yamamoto, Daiki, Asakura, Mami, Hayakawa, Tohru, Mise, Shintaro, Matsumoto, Akinobu, and Ide, Toru

Abstract

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, alpha-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

Published
2020

2. A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings

Author

Daiki Yamamoto, Toru Ide, Akinobu Matsumoto, Tohru Hayakawa, Shintaro Mise, Mami Asakura, and Minako Hirano

Subjects
BK channel, Materials science, single-channel recording, lcsh:Mechanical engineering and machinery, KcsA potassium channel, 02 engineering and technology, Article, 03 medical and health sciences, chemistry.chemical_compound, lcsh:TJ1-1570, Electrical and Electronic Engineering, Lipid bilayer, Ion channel, Ion transporter, 030304 developmental biology, 0303 health sciences, Alamethicin, biology, Mechanical Engineering, 021001 nanoscience & nanotechnology, Potassium channel, lipid bilayer, chemistry, Control and Systems Engineering, ion channel, Gramicidin, biology.protein, Biophysics, 0210 nano-technology
Abstract

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, &alpha, hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

Published
2020

3. A Lipid Bilayer Formed on a Hydrogel Bead for Single Ion Channel Recordings.

Author

Hirano M, Yamamoto D, Asakura M, Hayakawa T, Mise S, Matsumoto A, and Ide T

Abstract

Ion channel proteins play important roles in various cell functions, making them attractive drug targets. Artificial lipid bilayer recording is a technique used to measure the ion transport activities of channel proteins with high sensitivity and accuracy. However, the measurement efficiency is low. In order to improve the efficiency, we developed a method that allows us to form bilayers on a hydrogel bead and record channel currents promptly. We tested our system by measuring the activities of various types of channels, including gramicidin, alamethicin, α-hemolysin, a voltage-dependent anion channel 1 (VDAC1), a voltage- and calcium-activated large conductance potassium channel (BK channel), and a potassium channel from Streptomyces lividans (KcsA channel). We confirmed the ability for enhanced measurement efficiency and measurement system miniaturizion.

Published
2020
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