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Carbon dots (CDots) are generally defined as small carbon nanoparticles (CNPs) with effective surface passivation, for which the classical synthesis is the functionalization of pre-existing CNPs with organic molecules. However, "dot" samples produced by "one-pot" thermal carbonization of organic precursors are also popular in the literature. These carbonization-produced samples may contain nano-carbon domains embedded in organic matters from the precursors that survived the thermal processing, which may be considered and denoted as "nano-carbon/organic hybrids". Recent experimental evidence indicated that the two different kinds of dot samples are largely divergent in their photo-induced antibacterial functions. In this work, three representative carbonization-produced samples from the precursor of citric acid–oligomeric polyethylenimine mixture with processing conditions of 200 °C for 3 h (CS200), 330 °C for 6 h (CS330), and microwave heating (CSMT) were compared with the classically synthesized CDots on their photo-induced antiviral activities. The results suggest major divergences in the activities between the different samples. Interestingly, CSMT also exhibited significant differences between antibacterial and antiviral activities. The mechanistic origins of the divergences were explored, with the results of different antimicrobial activities among the hybrid samples rationalized in terms of the degree of carbonization in the sample production and the different sample structural and morphological characteristics. [ABSTRACT FROM AUTHOR]

Infectious keratitis is a vision-threatening microbial infection. The increasing antimicrobial resistance and the fact that severe cases often evolve into corneal perforation necessitate the development of alternative therapeutics for effective medical management. Genipin, a natural crosslinker, was recently shown to exert antimicrobial effects in an ex vivo model of microbial keratitis, highlighting its potential to serve as a novel treatment for infectious keratitis. This study aimed to evaluate the antimicrobial and anti-inflammatory effects of genipin in an in vivo model of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa) keratitis. Clinical scores, confocal microscopy, plate count, and histology were carried out to evaluate the severity of keratitis. To assess the effect of genipin on inflammation, the gene expression of pro- and anti-inflammatory factors, including matrix metalloproteinases (MMPs), were evaluated. Genipin treatment alleviated the severity of bacterial keratitis by reducing bacterial load and repressing neutrophil infiltration. The expression of interleukin 1B (IL1B), interleukin 6 (IL6), interleukin 8 (IL8), interleukin 15 (IL15), tumor necrosis factor-α (TNF-α), and interferon γ (IFNγ), as well as MMP2 and MMP9, were significantly reduced in genipin-treated corneas. Genipin promoted corneal proteolysis and host resistance to S. aureus and P. aeruginosa infection by suppressing inflammatory cell infiltration, regulating inflammatory mediators, and downregulating the expression of MMP2 and MMP9. [ABSTRACT FROM AUTHOR]

States of natural dormancy include estivation and hibernation. Ampullariids are exemplary because they undergo estivation when deprived of water or hibernation when exposed to very low temperatures. Regardless of the condition, ampullariids show increased endogenous antioxidant defenses, anticipating the expected respiratory burst during reoxygenation after reactivation, known as "Preparation for Oxidative Stress (POS)". In this work, we tested the POS hypothesis for changes in the blood and hemocytes of the bimodal breather Pomacea canaliculata (Ampullariidae) induced at experimental estivation and hibernation. We described respiratory (hemocyanin, proteins, lactate), antioxidant (GSH, uric acid, SOD, CAT, GST), and immunological (hemocyte levels, ROS production) parameters. We showed that, although the protein level remains unchanged in all experimental groups, hemocyanin increases in response to estivation. Furthermore, lactate remains unchanged in challenged snails, suggesting an aerobic metabolism during short-term challenges. Blood uric acid increases during estivation and arousal from estivation or hibernation, supporting the previously proposed antioxidant role. Regarding hemocytes, we showed that the total population increases with all challenges, and granulocytes increase during hibernation. We further showed that hibernation affects ROS production by hemocytes, possibly through mitochondrial inhibition. This study contributed to the knowledge of the adaptive strategies of ampullariids to tolerate adverse environmental conditions. [ABSTRACT FROM AUTHOR]

Advances in data generation and acquisition have resulted in a volume of available data of such magnitude that our ability to interpret and extract valuable knowledge from them has been surpassed. Our capacity to analyze data is hampered not only by their amount or their dimensionality, but also by their relationships and by the complexity of the systems they model. Compound graphs allow us to represent the existing relationships between nodes that are themselves hierarchically structured, so they are a natural substrate to support multiscale analysis of complex graphs. This paper presents Carbonic, a framework for interactive multiscale visual exploration and editing of compound graphs that incorporates several strategies for complexity management. It combines the representation of graphs at multiple levels of abstraction, with techniques for reducing the number of visible elements and for reducing visual cluttering. This results in a tool that allows both the exploration of existing graphs and the visual creation of compound graphs following a top-down approach that allows simultaneously observing the entities and their relationships at different scales. The results show the applicability of the developed framework to two use cases, demonstrating the usefulness of Carbonic for moving from information to knowledge. [ABSTRACT FROM AUTHOR]

Land use and land cover (LULC) change has become an important research topic for global environmental change and sustainable development. As an important part of worldwide land conservation, sustainable development and management of water resources, developing countries must ensure the use of innovative technology and tools that support their various decision making systems. This study provides the most recent LULC change analysis for the last six years (2015–2021) of Coatzacoalcos, Veracruz, Mexico, one of the most important petrochemical cities in the world and host of the ongoing Interoceanic Corridor project. The analysis was carried out using Landsat 8 Operational Land Imager (OLI) satellite images, ancillary data and ground-based surveys and the Normalized Difference Vegetation Index (NDVI) to identify and to ameliorate the discrimination between four main macro-classes and fourteen classes. The LULC classification was performed using the maximum likelihood classifier (MLC) to produce maps for each year, as it was found to be the best approach when compared to minimum distance (MDM) and spectral angle mapping (SAM) methods. The macro-classes were water, built-up, vegetation and bare soil, whereas the classes were an improved classification within those. Our study achieved both user accuracy (UA) and producer accuracy (PA) above 90% for the proposed macro-classes and classes. The average Kappa coefficient for macro-classes was 0.93, while for classes it was 0.96, both comparable to previous studies. The results from the LULC analysis show that residential, industry and commercial areas slowed down their growth throughout the study period. These changes were associated with socio-economical drivers such as insecurity and lack of economic investments. Groves and trees presented steady behaviors, with small increments during the five-year period. Swamps, on the other hand, significantly degraded, being about 2% of the study area in 2015 and 0.93% in 2021. Dunes and medium and high vegetation densities ( ∼ 80 % ) transitioned mostly to low vegetation densities. This behavior is associated with rainfall below the annual reference and increments of surface runoff due to the loss of vegetation cover. Lastly, the present study seeks to highlight the importance of remote sensing for a better understanding of the dynamics between human–nature interactions and to provide information to assist planners and decision-makers for more sustainable land development. [ABSTRACT FROM AUTHOR]

Today, dendrimers are the main nanoparticle applied to drug delivery systems. The physicochemical characteristics of dendrimers and their versatility structural modification make them attractive to applied as a platform to bioactive molecules transport. Nanoformulations based on dendrimers enhance low solubility drugs, arrival to the target tissue, drugs bioavailability, and controlled release. This review describes the latter approaches on the transport of bioactive molecules based on dendrimers. The review focus is on the last therapeutic strategies addressed by dendrimers conjugated with bioactive molecules. A brief review of the latest studies in therapies against cancer and cardiovascular diseases, as well as future projections in the area, are addressed.