43 results on '"Rodríguez, Cristina"'
Search Results
2. Monkeypox and HIV in the canary islands: A different pattern in a mobile population
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Betancort-Plata, Christian, Lopez-Delgado, Laura, Jaen-Sanchez, Nieves, Tosco-Nunez, Tomas, Suarez-Hormiga, Laura, Lavilla-Salgado, Carmen, Pisos-Alamo, Elena, Hernandez-Betancor, Araceli, Hernandez-Cabrera, Michele, Carranza-Rodriguez, Cristina, Briega-Molina, Marta, and Perez-Arellano, Jose-Luis
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- 2022
3. Cellular Immunity of SARS-CoV-2 in the Borriana COVID-19 Cohort: A Nested Case–Control Study.
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Domènech-Montoliu, Salvador, Puig-Barberà, Joan, Pac-Sa, María Rosario, Orrico-Sanchéz, Alejandro, Gómez-Lanas, Lorna, Sala-Trull, Diego, Domènech-Leon, Carmen, Del Rio-González, Alba, Sánchez-Urbano, Manuel, Satorres-Martinez, Paloma, Aparisi-Esteve, Laura, Badenes-Marques, Gema, Blasco-Gari, Roser, Casanova-Suarez, Juan, Gil-Fortuño, María, Hernández-Pérez, Noelia, Jovani-Sales, David, López-Diago, Laura, Notari-Rodríguez, Cristina, and Pérez-Olaso, Oscar
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CELLULAR immunity ,SARS-CoV-2 ,BLOOD groups ,COVID-19 ,COVID-19 vaccines ,BLOOD group antigens ,ABO blood group system - Abstract
Our goal was to determine the cellular immune response (CIR) in a sample of the Borriana COVID-19 cohort (Spain) to identify associated factors and their relationship with infection, reinfection and sequelae. We conducted a nested case–control study using a randomly selected sample of 225 individuals aged 18 and older, including 36 individuals naïve to the SARS-CoV-2 infection and 189 infected patients. We employed flow-cytometry–based immunoassays for intracellular cytokine staining, using Wuhan and BA.2 antigens, and chemiluminescence microparticle immunoassay to detect SARS-CoV-2 antibodies. Logistic regression models were applied. A total of 215 (95.6%) participants exhibited T-cell response (TCR) to at least one antigen. Positive responses of CD4+ and CD8+ T cells were 89.8% and 85.3%, respectively. No difference in CIR was found between naïve and infected patients. Patients who experienced sequelae exhibited a higher CIR than those without. A positive correlation was observed between TCR and anti-spike IgG levels. Factors positively associated with the TCR included blood group A, number of SARS-CoV-2 vaccine doses received, and anti-N IgM; factors inversely related were the time elapsed since the last vaccine dose or infection, and blood group B. These findings contribute valuable insights into the nuanced immune landscape shaped by SARS-CoV-2 infection and vaccination. [ABSTRACT FROM AUTHOR]
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- 2024
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4. Bioelectrical Impedance Vector Analysis, Nutritional Ultrasound ® , and Handgrip Strength as Innovative Methods for Monitoring Critical Anorexia Nervosa Physical Recovery: A Pilot Study.
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Romero-Márquez, Jose M., Novo-Rodríguez, María, Novo-Rodríguez, Cristina, Siles-Guerrero, Víctor, Herrera-Montes, Isabel, Navarro-Pelayo, Francisco Garzón, López-de-la-Torre-Casares, Martín, and Muñoz-Garach, Araceli
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Eating disorders (EDs) manifest as persistent disruptions in eating habits or related behaviors, significantly impacting physical health and psychosocial well-being. Nutritional assessment in ED patients is crucial for monitoring treatment efficacy. While dual-energy X-ray absorptiometry (DEXA) remains standard, interest in alternative methods such as bioelectrical impedance vector analysis (BIVA) and Nutritional Ultrasound
® (NU) has risen due to their affordability and portability. Additionally, hand dynamometry offers a user-friendly approach to assessing grip strength (HGS), indicative of nutritional status. A prospective study was carried out to evaluate the utility of BIVA, NU® , and HGS in 43 female AN patients. Measurements were taken at baseline and hospital discharge. A total of 41 patients completed the study. After the intervention, numerous BIVA-related parameters such as fat (3.5 ± 2 kg vs. 5.3 ± 2.7 kg, p < 0.001) and free fat mass (33.9 ± 3.8 kg vs. 37.5 ± 4.1 kg, p < 0.001) were partially restored. Similarly, Nutritional Ultrasound® showed promising results in assessing body composition changes such as total abdominal fat tissue (0.5 ± 0.3 cm vs. 0.9 ± 0.3 cm, p < 0.05). In the same way, rectus femoris cross-sectional area values correlated with clinical outcomes such as free fat mass (0.883, p < 0.05) and appendicular muscle mass (0.965, p < 0.001). HGS reached the normality percentile after the intervention (21.6 ± 9.1 kg vs. 25.9 ± 12.3 kg, p < 0.05), demonstrating a significant association between grip strength and body composition parameters such as free fat mass (0.658, p < 0.001) and appendicular muscle mass (0.482, p < 0.001). Incorporating BIVA-, NU® -, and HGS-enhanced nutritional assessment into the treatment of AN patients offers cost-effective, portable, and non-invasive alternatives to DEXA. These techniques offer valuable insights into changes in body composition and nutritional status, which, in turn, facilitate treatment monitoring and contribute to improved patient outcomes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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5. Lysyl Oxidase in Ectopic Cardiovascular Calcification: Role of Oxidative Stress.
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Ballester-Servera, Carme, Alonso, Judith, Cañes, Laia, Vázquez-Sufuentes, Paula, García-Redondo, Ana B., Rodríguez, Cristina, and Martínez-González, José
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LYSYL oxidase ,OXIDATIVE stress ,CALCIFICATION ,AORTIC valve diseases ,VASCULAR smooth muscle ,INTERSTITIAL cells - Abstract
Lysyl oxidase (LOX)-mediated extracellular matrix crosslinking modulates calcification in atherosclerosis and aortic valve disease; however, this enzyme also induces oxidative stress. We addressed the contribution of LOX-dependent oxidative stress to cardiovascular calcification. LOX is upregulated in human-calcified atherosclerotic lesions and atheromas from atherosclerosis-challenged LOX transgenic mice (TgLOX
VSMC ) and colocalized with a marker of oxidative stress (8-oxo-deoxyguanosine) in vascular smooth muscle cells (VSMCs). Similarly, in calcific aortic valves, high LOX expression was detected in valvular interstitial cells (VICs) positive for 8-oxo-deoxyguanosine, while LOX and LOXL2 expression correlated with osteogenic markers (SPP1 and RUNX2) and NOX2. In human VICs, mito-TEMPO and TEMPOL attenuated the increase in superoxide anion levels and the mineralization induced by osteogenic media (OM). Likewise, in OM-exposed VICs, β-aminopropionitrile (a LOX inhibitor) ameliorated both oxidative stress and calcification. Gain- and loss-of-function approaches in VICs demonstrated that while LOX silencing negatively modulates oxidative stress and calcification induced by OM, lentiviral LOX overexpression exacerbated oxidative stress and VIC calcification, effects that were prevented by mito-TEMPO, TEMPOL, and β-aminopropionitrile. Our data indicate that LOX-induced oxidative stress participates in the procalcifying effects of LOX activity in ectopic cardiovascular calcification, and highlight the multifaceted role played by LOX isoenzymes in cardiovascular diseases. [ABSTRACT FROM AUTHOR]- Published
- 2024
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6. Toxoplasma gondii Infection in Humans: A Comprehensive Approach Involving the General Population, HIV-Infected Patients and Intermediate-Duration Fever in the Canary Islands, Spain.
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Carranza-Rodríguez, Cristina, Bolaños-Rivero, Margarita, and Pérez-Arellano, José-Luis
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TOXOPLASMA gondii , *HIV-positive persons , *IMMUNOCOMPROMISED patients , *ISLANDS , *FEVER - Abstract
A prior investigation in 1993 identified a high seroprevalence of toxoplasmosis (63%) in the Canary Islands. This study aims to assess the current prevalence of the disease in diverse population groups. The study was based on a population-scale screening involving 273 residents utilizing T. gondii IgG ELISA and a 20 year retrospective study (1998–2018). This included AIDS/HIV outpatients (1357, of which 324 were residents), AIDS/HIV hospitalized patients (741) and patients with fever of intermediate duration (158). The seroprevalence in the resident population was 37%, with significant differences between islands. Among resident outpatients with AIDS/HIV, 14.2% had specific anti-T. gondii IgG, and three had anti-T. gondii IgM; however, IgG avidity testing indicated non-active infection. In patients hospitalized for AIDS/HIV, T. gondii causing encephalitis was detected in 2%. Among patients with fever of intermediate duration, 28.5% were positive for T. gondii IgG, and four also showed IgM positivity, although the infection was non-active. The study reveals a decrease in human toxoplasmosis over the past 30 years. However, the current seroprevalence, which stands at 37%, together with the substantial risk that T. gondii represents for immunocompromised individuals, highlights the need to implement preventive and control strategies to control the threat that this infection can pose to public health in the Canary Islands population. [ABSTRACT FROM AUTHOR]
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- 2024
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7. Spanish HCMV Seroprevalence in the 21st Century.
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Álvarez-Heredia, Pablo, Reina-Alfonso, Irene, Domínguez-del-Castillo, José Joaquín, Hassouneh, Fakhri, Gutiérrez-González, Carmen, Batista-Duharte, Alexander, Pérez, Ana-Belén, Sarramea, Fernando, Jaén-Moreno, María José, Camacho-Rodríguez, Cristina, Tarazona, Raquel, Solana, Rafael, Molina, Juan, and Pera, Alejandra
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TWENTY-first century ,HEALTH planning ,SEROPREVALENCE ,ANTIBODY titer ,CHILDBEARING age - Abstract
Human cytomegalovirus (HCMV) is linked to age-related diseases like cardiovascular disease, neurodegenerative conditions, and cancer. It can also cause congenital defects and severe illness in immunocompromised individuals. Accurate HCMV seroprevalence assessment is essential for public health planning and identifying at-risk individuals. This is the first HCMV seroprevalence study conducted in the general Spanish adult population in 30 years. We studied HCMV seroprevalence and HCMV IgG antibody titres in healthy adult donors (HDs) and HCMV-related disease patients from 2010 to 2013 and 2020 to 2023, categorized by sex and age. We compared our data with 1993 and 1999 studies in Spain. The current HCMV seroprevalence among HDs in Spain is 73.48%. In women of childbearing age, HCMV seroprevalence has increased 1.4-fold in the last decade. HCMV-seropositive individuals comprise 89.83% of CVD patients, 69% of SMI patients, and 70.37% of COVID-19 patients. No differences in HCMV seroprevalence or HCMV IgG antibody titres were observed between patients and HDs. A significant reduction in Spanish HCMV seroprevalence among HDs was observed in 1993. However, women of childbearing age have shown an upturn in the last decade that may denote a health risk in newborns and a change in HCMV seroprevalence trends. [ABSTRACT FROM AUTHOR]
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- 2024
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8. PDE4 Phosphodiesterases in Cardiovascular Diseases: Key Pathophysiological Players and Potential Therapeutic Targets.
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Puertas-Umbert, Lídia, Alonso, Judith, Hove-Madsen, Leif, Martínez-González, José, and Rodríguez, Cristina
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PHOSPHODIESTERASES ,CARDIOVASCULAR diseases ,CYCLIC nucleotides ,DRUG target ,VASCULAR smooth muscle ,HOMEOSTASIS ,CARDIOVASCULAR development - Abstract
3′,5′-cyclic adenosine monophosphate (cAMP) is a second messenger critically involved in the control of a myriad of processes with significant implications for vascular and cardiac cell function. The temporal and spatial compartmentalization of cAMP is governed by the activity of phosphodiesterases (PDEs), a superfamily of enzymes responsible for the hydrolysis of cyclic nucleotides. Through the fine-tuning of cAMP signaling, PDE4 enzymes could play an important role in cardiac hypertrophy and arrhythmogenesis, while it decisively influences vascular homeostasis through the control of vascular smooth muscle cell proliferation, migration, differentiation and contraction, as well as regulating endothelial permeability, angiogenesis, monocyte/macrophage activation and cardiomyocyte function. This review summarizes the current knowledge and recent advances in understanding the contribution of the PDE4 subfamily to cardiovascular function and underscores the intricate challenges associated with targeting PDE4 enzymes as a therapeutic strategy for the management of cardiovascular diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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9. Preclinical Evaluation of [ 155/161 Tb]Tb-Crown-TATE—A Novel SPECT Imaging Theranostic Agent Targeting Neuroendocrine Tumours.
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Wharton, Luke, McNeil, Scott W., Merkens, Helen, Yuan, Zheliang, Van de Voorde, Michiel, Engudar, Gokce, Ingham, Aidan, Koniar, Helena, Rodríguez-Rodríguez, Cristina, Radchenko, Valery, Ooms, Maarten, Kunz, Peter, Bénard, François, Schaffer, Paul, and Yang, Hua
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NEUROENDOCRINE tumors ,SINGLE-photon emission computed tomography ,RADIOACTIVE decay ,COMPUTED tomography ,RADIOACTIVE tracers ,COLLIMATORS - Abstract
Terbium radioisotopes (
149 Tb,152 Tb,155 Tb,161 Tb) offer a unique class of radionuclides which encompass all four medicinally relevant nuclear decay modalities (α, β+ , γ, β− /e− ), and show high potential for the development of element-matched theranostic radiopharmaceuticals. The goal of this study was to design, synthesise, and evaluate the suitability of crown-TATE as a new peptide-conjugate for radiolabelling of [155 Tb]Tb3+ and [161 Tb]Tb3+ , and to assess the imaging and pharmacokinetic properties of each radiotracer in tumour-bearing mice. [155 Tb]Tb-crown-TATE and [161 Tb]Tb-crown-TATE were prepared efficiently under mild conditions, and exhibited excellent stability in human serum (>99.5% RCP over 7 days). Longitudinal SPECT/CT images were acquired for155 Tb- and161 Tb- labelled crown-TATE in male NRG mice bearing AR42J tumours. The radiotracers, [155 Tb]Tb-crown-TATE and [161 Tb]Tb-crown-TATE, showed high tumour targeting (32.6 and 30.0 %ID/g, respectively) and minimal retention in non-target organs at 2.5 h post-administration. Biodistribution studies confirmed the SPECT/CT results, showing high tumour uptake (38.7 ± 8.0 %ID/g and 38.5 ± 3.5 %ID/g, respectively) and favourable tumour-to-background ratios. Blocking studies further confirmed SSTR2-specific tumour accumulation. Overall, these findings suggest that crown-TATE has great potential for element-matched molecular imaging and radionuclide therapy using155 Tb and161 Tb. [ABSTRACT FROM AUTHOR]- Published
- 2023
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10. Correction: Chaparro et al. Incidence, Clinical Characteristics and Management of Inflammatory Bowel Disease in Spain: Large-Scale Epidemiological Study. J. Clin. Med. 2021, 10, 2885
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Chaparro, María, Garre, Ana, Núñez Ortiz, Andrea, Diz-Lois Palomares, María Teresa, Rodríguez, Cristina, Riestra, Sabino, Vela, Milagros, Benítez, José Manuel, Fernández Salgado, Estela, Sánchez Rodríguez, Eugenia, Hernández, Vicent, Ferreiro-Iglesias, Rocío, Ponferrada Díaz, Ángel, Barrio, Jesús, Huguet, José María, Sicilia, Beatriz, Martín-Arranz, María Dolores, Calvet, Xavier, Ginard, Daniel, Alonso-Abreu, Inmaculada, Fernández-Salazar, Luis, Varela Trastoy, Pilar, Rivero, Montserrat, Vera-Mendoza, Isabel, Vega, Pablo, Navarro, Pablo, Sierra, Mónica, Cabriada, José Luis, Aguas, Mariam, Vicente, Raquel, Navarro-Llavat, Mercè, Echarri, Ana, Gomollón, Fernando, Guerra Del Río, Elena, Piñero, Concepción, Casanova, María José, Spicakova, Katerina, Ortiz de Zarate, Jone, Torrella Cortés, Emilio, Gutiérrez, Ana, Alonso-Galán, Horacio, Hernández-Martínez, Álvaro, Marrero, José Miguel, Lorente Poyatos, Rufo, Calafat, Margalida, Martí Romero, Lidia, Robledo, Pilar, Bosch, Orencio, Jiménez, Nuria, Esteve Comas, María, Duque, José María, Fuentes Coronel, Ana María, Josefa Sampedro, Manuela, Sesé Abizanda, Eva, Herreros Martínez, Belén, Pozzati, Liliana, Fernández Rosáenz, Hipólito, Crespo Suarez, Belén, López Serrano, Pilar, Lucendo, Alfredo J, Muñoz Vicente, Margarita, Bermejo, Fernando, Ramírez Palanca, José Joaquín, Menacho, Margarita, Carmona, Amalia, Camargo, Raquel, Torra Alsina, Sandra, Maroto, Nuria, Nerín de la Puerta, Juan, Castro, Elena, Marín-Jiménez, Ignacio, Botella, Belén, Sapiña, Amparo, Cruz, Noelia, Forcelledo, José Luis F, Bouhmidi, Abdel, Castaño-Milla, Carlos, Opio, Verónica, Nicolás, Isabel, Kutz, Marcos, Abraldes Bechiarelli, Alfredo, Gordillo, Jordi, Ber, Yolanda, Torres Domínguez, Yolanda, Novella Durán, María Teresa, Rodríguez Mondéjar, Silvia, Martínez-Cerezo, Francisco J, Kolle, Lilyan, Sabat, Miriam, Ledezma, Cesar, Iyo, Eduardo, Roncero, Óscar, Irisarri, Rebeca, Lluis, Laia, Blázquez Gómez, Isabel, Zapata, Eva María, José Alcalá, María, Martínez Pascual, Cristina, Montealegre, María, Mata, Laura, Monrobel, Ana, Hernández Camba, Alejandro, Hernández, Luis, Tejada, María, Mir, Alberto, Galve, María Luisa, Soler, Marta, Hervías, Daniel, Gómez-Valero, José Antonio, Barreiro-de Acosta, Manuel, Rodríguez-Artalejo, Fernando, García-Esquinas, Esther, Gisbert, Javier P, and On Behalf Of The EpidemIBD Study Group Of Geteccu
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General Medicine - Abstract
The authors wish to make the following corrections to this paper [...]
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- 2022
11. Opposite effects of moderate and extreme Cx43 deficiency in conditional Cx43-deficient mice on angiotensin II-induced cardiac fibrosis
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Valls de Lacalle, Laura, Rodríguez, Cristina, Negre-Pujol, Corall, Varona, Saray, Antoni Valera Cañellas, Consegal, Marta, Martínez-González, Jose, Rodríguez-Sinovas, Antonio, Universitat Autònoma de Barcelona, [Valls-Lacalle L, Negre-Pujol C, Valera-Cañellas A, Consegal M, Rodríguez-Sinovas A] Servei de Cardiologia, Vall d’Hebron Hospital Universitari, Barcelona, Spain. Grup de Recerca en Malalties Cardiovasculars, Vall d’Hebron Institut de Recerca (VHIR), Barcelona, Spain. Departament de Medicina, Universitat Autònoma de Barcelona, Barcelona, Spain. Centro de Investigación Biomédica en Red sobre Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain. [Rodríguez C] Centro de Investigación Biomédica en Red sobre Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain. Institut de Recerca del Hospital de la Santa Creu i Sant Pau (IIB-Sant Pau), Barcelona, Spain. [Varona S] Centro de Investigación Biomédica en Red sobre Enfermedades Cardiovasculares (CIBERCV), Madrid, Spain. Instituto de Investigaciones Biomédicas de Barcelona (IIBB-CSIC), Barcelona, Spain. Institut de Recerca del Hospital de la Santa Creu i Sant Pau (IIB-Sant Pau), Barcelona, Spain, Vall d'Hebron Barcelona Hospital Campus, Fundació La Marató de TV3, Instituto de Salud Carlos III, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Generalitat de Catalunya, Rodríguez, Cristina [0000-0002-6472-5647], Rodríguez-Sinovas, Antonio [0000-0003-2930-8773], Rodríguez, Cristina, and Rodríguez-Sinovas, Antonio
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0301 basic medicine ,collagen ,Cardiac fibrosis ,Connexin ,Muscle Proteins ,030204 cardiovascular system & hematology ,angiotensin ii ,Muscle hypertrophy ,connexin 43 ,Mice ,0302 clinical medicine ,Fibrosis ,Macrophage ,lcsh:QH301-705.5 ,Mice, Knockout ,Chemistry ,Angiotensin II ,Cell Differentiation ,General Medicine ,Eukaryota::animales::grupos de población animal::animales modificados genéticamente::ratones transgénicos::ratones noqueados [ORGANISMOS] ,Cardiovascular Diseases::Heart Diseases::Cardiomyopathies [DISEASES] ,cardiovascular system ,Collagen ,biological phenomena, cell phenomena, and immunity ,Cardiomyopathies ,hypertrophy ,hormones, hormone substitutes, and hormone antagonists ,medicine.medical_specialty ,p38 mitogen-activated protein kinases ,Miocardi - Malalties ,Amino Acids, Peptides, and Proteins::Proteins::Membrane Proteins::Membrane Transport Proteins::Connexins::Connexin 43 [CHEMICALS AND DRUGS] ,Article ,Eukaryota::Animals::Animal Population Groups::Animals, Genetically Modified::Mice, Transgenic::Mice, Knockout [ORGANISMS] ,03 medical and health sciences ,Internal medicine ,medicine ,aminoácidos, péptidos y proteínas::proteínas::proteínas de membranas::proteínas de transporte de membrana::conexinas::conexina 43 [COMPUESTOS QUÍMICOS Y DROGAS] ,Animals ,Myocardium ,fibrosis ,Hypertrophy ,Fibroblasts ,medicine.disease ,Connexines ,030104 developmental biology ,Endocrinology ,lcsh:Biology (General) ,Connexin 43 ,enfermedades cardiovasculares::enfermedades cardíacas::miocardiopatías [ENFERMEDADES] ,Myocardial fibrosis ,sense organs ,Models animals en la investigació - Abstract
Connexin 43 (Cx43) is essential for cardiac electrical coupling, but its effects on myocardial fibrosis is controversial. Here, we analyzed the role of Cx43 in myocardial fibrosis caused by angiotensin II (AngII) using Cx43fl/fl and Cx43Cre-ER(T)/fl inducible knock-out (Cx43 content: 50%) mice treated with vehicle or 4-hydroxytamoxifen (4-OHT) to induce a Cre-ER(T)-mediated global deletion of the Cx43 floxed allele. Myocardial collagen content was enhanced by AngII in all groups (n = 8&ndash, 10/group, p <, 0.05). However, animals with partial Cx43 deficiency (vehicle-treated Cx43Cre-ER(T)/fl) had a significantly higher AngII-induced collagen accumulation that reverted when treated with 4-OHT, which abolished Cx43 expression. The exaggerated fibrotic response to AngII in partially deficient Cx43Cre-ER(T)/fl mice was associated with enhanced p38 MAPK activation and was not evident in Cx43 heterozygous (Cx43+/-) mice. In contrast, normalization of interstitial collagen in 4-OHT-treated Cx43Cre-ER(T)/fl animals correlated with enhanced MMP-9 activity, IL-6 and NOX2 mRNA expression, and macrophage content, and with reduced &alpha, SMA and SM22&alpha, in isolated fibroblasts. In conclusion, our data demonstrates an exaggerated, p38 MAPK-dependent, fibrotic response to AngII in partially deficient Cx43Cre-ER(T)/fl mice, and a paradoxical normalization of collagen deposition in animals with an almost complete Cx43 ablation, an effect associated with increased MMP-9 activity and inflammatory response and reduced fibroblasts differentiation.
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- 2019
12. A Scoping Review of the Influence of Mindfulness on Men's Sexual Activity.
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Valderrama Rodríguez, María Fernanda, Sánchez-Sánchez, Laura C., García-Montes, José Manuel, and Petisco-Rodríguez, Cristina
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- 2023
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13. Retinal Pigment Epithelium-Secreted VEGF-A Induces Alpha-2-Macroglobulin Expression in Endothelial Cells.
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Lehmann, Guillermo L., Ginsberg, Michael, Nolan, Daniel J., Rodríguez, Cristina, Martínez-González, José, Zeng, Shemin, Voigt, Andrew P., Mullins, Robert F., Rafii, Shahin, Rodriguez-Boulan, Enrique, and Benedicto, Ignacio
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RHODOPSIN ,ENDOTHELIAL cells ,MACULAR degeneration ,RETINAL blood vessels ,VISION ,RETINAL diseases ,EXTRACELLULAR matrix - Abstract
Alpha-2-macroglobulin (A2M) is a protease inhibitor that regulates extracellular matrix (ECM) stability and turnover. Here, we show that A2M is expressed by endothelial cells (ECs) from human eye choroid. We demonstrate that retinal pigment epithelium (RPE)-conditioned medium induces A2M expression specifically in ECs. Experiments using chemical inhibitors, blocking antibodies, and recombinant proteins revealed a key role of VEGF-A in RPE-mediated A2M induction in ECs. Furthermore, incubation of ECs with RPE-conditioned medium reduces matrix metalloproteinase-2 gelatinase activity of culture supernatants, which is partially restored after A2M knockdown in ECs. We propose that dysfunctional RPE or choroidal blood vessels, as observed in retinal diseases such as age-related macular degeneration, may disrupt the crosstalk mechanism we describe here leading to alterations in the homeostasis of choroidal ECM, Bruch's membrane and visual function. [ABSTRACT FROM AUTHOR]
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- 2022
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14. Incidence, Clinical Characteristics and Management of Inflammatory Bowel Disease in Spain: Large-Scale Epidemiological Study
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Chaparro, María, Garre, Ana, Núñez Ortiz, Andrea, Diz-Lois Palomares, María Teresa, Rodríguez, Cristina, Riestra, Sabino, Vela, Milagros, Benítez, José Manuel, Fernández Salgado, Estela, Sánchez Rodríguez, Eugenia, Hernández, Vicent, Ferreiro-Iglesias, Rocío, Ponferrada Díaz, Ángel, Barrio, Jesús, Huguet, José María, Sicilia, Beatriz, Martín-Arranz, María Dolores, Calvet, Xavier, Ginard, Daniel, Alonso-Abreu, Inmaculada, Fernández-Salazar, Luis, Varela Trastoy, Pilar, Rivero, Montserrat, Vera-Mendoza, Isabel, Vega, Pablo, Navarro, Pablo, Sierra, Mónica, Cabriada, José Luis, Aguas, Mariam, Vicente, Raquel, Navarro-Llavat, Mercè, Echarri, Ana, Gomollón, Fernando, Guerra Del Río, Elena, Piñero, Concepción, Casanova, María José, Spicakova, Katerina, Ortiz de Zarate, Jone, Torrella Cortés, Emilio, Gutiérrez, Ana, Alonso-Galán, Horacio, Hernández-Martínez, Álvaro, Marrero, José Miguel, Lorente Poyatos, Rufo, Calafat, Margalida, Martí Romero, Lidia, Robledo, Pilar, Bosch, Orencio, Jiménez, Nuria, Esteve Comas, María, Duque, José María, Fuentes Coronel, Ana María, Josefa Sampedro, Manuela, Sesé Abizanda, Eva, Herreros Martínez, Belén, Pozzati, Liliana, Fernández Rosáenz, Hipólito, Crespo Suarez, Belén, López Serrano, Pilar, Lucendo, Alfredo J, Muñoz Vicente, Margarita, Bermejo, Fernando, Ramírez Palanca, José Joaquín, Menacho, Margarita, Carmona, Amalia, Camargo, Raquel, Torra Alsina, Sandra, Maroto, Nuria, Nerín de la Puerta, Juan, Castro, Elena, Marín-Jiménez, Ignacio, Botella, Belén, Sapiña, Amparo, Cruz, Noelia, Forcelledo, José Luis F, Bouhmidi, Abdel, Castaño-Milla, Carlos, Opio, Verónica, Nicolás, Isabel, Kutz, Marcos, Abraldes Bechiarelli, Alfredo, Gordillo, Jordi, Ber, Yolanda, Torres Domínguez, Yolanda, Novella Durán, María Teresa, Rodríguez Mondéjar, Silvia, Martínez-Cerezo, Francisco J, Kolle, Lilyan, Sabat, Miriam, Ledezma, Cesar, Iyo, Eduardo, Roncero, Óscar, Irisarri, Rebeca, Lluis, Laia, Blázquez Gómez, Isabel, Zapata, Eva María, José Alcalá, María, Martínez Pascual, Cristina, Montealegre, María, Mata, Laura, Monrobel, Ana, Hernández Camba, Alejandro, Hernández, Luis, Tejada, María, Mir, Alberto, Galve, María Luisa, Soler, Marta, Hervías, Daniel, Gómez-Valero, José Antonio, Barreiro-de Acosta, Manuel, Rodríguez-Artalejo, Fernando, García-Esquinas, Esther, Gisbert, Javier P, On Behalf Of The EpidemIBD Study Group Of Geteccu, and Servicio de Digestivo. Hospital Universitario de Fuenlabrada
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Crohn’s disease ,medicine.medical_specialty ,Population ,Disease ,Inflammatory bowel disease ,digestive system ,Article ,03 medical and health sciences ,0302 clinical medicine ,Interquartile range ,inflammatory bowel disease ,Internal medicine ,Epidemiology ,Medicine ,education ,Crohn’s disease, epidemiology, incidence, inflammatory bowel disease, ulcerative colitis ,ulcerative colitis ,Crohn's disease ,education.field_of_study ,business.industry ,Incidence (epidemiology) ,General Medicine ,medicine.disease ,Ulcerative colitis ,digestive system diseases ,030220 oncology & carcinogenesis ,incidence ,030211 gastroenterology & hepatology ,epidemiology ,business - Abstract
(1) Aims: To assess the incidence of inflammatory bowel disease (IBD) in Spain, to describe the main epidemiological and clinical characteristics at diagnosis and the evolution of the disease, and to explore the use of drug treatments. (2) Methods: Prospective, population-based nationwide registry. Adult patients diagnosed with IBD—Crohn’s disease (CD), ulcerative colitis (UC) or IBD unclassified (IBD-U)—during 2017 in Spain were included and were followed-up for 1 year. (3) Results: We identified 3611 incident cases of IBD diagnosed during 2017 in 108 hospitals covering over 22 million inhabitants. The overall incidence (cases/100,000 person-years) was 16 for IBD, 7.5 for CD, 8 for UC, and 0.5 for IBD-U, 53% of patients were male and median age was 43 years (interquartile range = 31–56 years). During a median 12-month follow-up, 34% of patients were treated with systemic steroids, 25% with immunomodulators, 15% with biologics and 5.6% underwent surgery. The percentage of patients under these treatments was significantly higher in CD than UC and IBD-U. Use of systemic steroids and biologics was significantly higher in hospitals with high resources. In total, 28% of patients were hospitalized (35% CD and 22% UC patients, p <, 0.01). (4) Conclusion: The incidence of IBD in Spain is rather high and similar to that reported in Northern Europe. IBD patients require substantial therapeutic resources, which are greater in CD and in hospitals with high resources, and much higher than previously reported. One third of patients are hospitalized in the first year after diagnosis and a relevant proportion undergo surgery.
- Published
- 2021
15. Cost Effectiveness of Quadrivalent Versus Trivalent Inactivated Influenza Vaccines for the Portuguese Elderly Population.
- Author
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Tavares, Diana, Mouriño, Helena, Rodríguez, Cristina Antón, and Saborido, Carlos Martín
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COST effectiveness ,OLDER people ,INFLUENZA vaccines ,COMBINED vaccines ,SWITCHING costs - Abstract
Background: quadrivalent inactivated vaccine (QIV) has replaced trivalent inactivated vaccine (TIV). In Portugal, TIV is free of charge for risk groups, including older adults (≥65 years old). In its turn, QIV—which provides broader protection as it includes an additional lineage B strain—was introduced in Portugal in October 2018; only since the 2019/20 influenza season has it been provided free of charge for risk groups. This study evaluates the cost effectiveness of switching from TIV to QIV, from the National Health Service perspective, in the Portuguese elderly mainland population. Methods: A decision tree model was developed to compare TIV and QIV, based on Portuguese hospitalization data for the 2015/16 influenza season. The primary health economic outcome under consideration was the incremental cost-effectiveness ratio (ICER). In addition, one-way sensitivity analysis and probabilistic sensitivity analysis were performed. Results: the high cost of QIV (approximately three times the cost of TIV) would lead to a total increment of EUR 5,283,047, and the resulting ICER would be EUR 26,403,007/QALY, above the usual willingness-to-pay threshold. Conclusions: from the National Health Service perspective, our findings reveal that QIV is not cost effective for the Portuguese elderly population due to the high cost. If the QIV costs were the same as the TIV, then QIV would be cost effective. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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16. Effect of a Science of Learning Course on Beliefs in Neuromyths and Neuroscience Literacy.
- Author
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Ferreira, Roberto A. and Rodríguez, Cristina
- Subjects
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LITERACY , *STUDENT teachers , *NEUROSCIENCES , *EXPERIMENTAL groups - Abstract
Misconceptions about the brain (neuromyths) among educators have been found across different countries, but little has been done to dispel them. The present study assessed the effect of a one-year Science of Learning (SoL) course on neuroscience literacy and beliefs in neuromyths in a sample of Chilean pre-service teachers. An experimental group of pre-service teachers, who took the SoL course as part of their university training, and a control group were needed for the study. Participants in both groups completed an online survey three times during the year (beginning, middle and end of year). The results showed that participants in both groups responded correctly to most assertions but held major misconceptions about the brain (Time 1), in line with previous studies. Regarding neuroscience literacy, participants in the experimental and control groups did not differ significantly at Time 1, but the experimental group showed significantly better performance than the control group at Time 2 and Time 3. Unlike neuroscience literacy, the results in neuromyth beliefs did not differ significantly by group at Time 1 and Time 2; however, at Time 3, the experimental group showed a significant decline in neuromyth beliefs. Overall, these results suggest that the SoL course significantly improved overall neuroscience literacy and reduced neuromyth belief among pre-service teachers, but the effect of the intervention was small. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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17. Effect of Internal Hydrogen on the Fatigue Crack Growth Rate in the Coarse-Grain Heat-Affected Zone of a CrMo Steel.
- Author
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Álvarez, Guillermo, Zafra, Alfredo, Belzunce, Francisco Javier, and Rodríguez, Cristina
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FATIGUE crack growth ,GRAIN ,WELDED joints ,STEEL welding ,HYDROGEN ,HEAT treatment - Abstract
The effect of internal hydrogen in the fatigue crack growth rate of the coarse grain region of a 2.25Cr1Mo steel welded joint was analyzed in this work. The microstructure of the coarse grain region was simulated by means of a heat treatment able to provide the same microstructure with a similar prior austenite grain size and hardness to the one in a real welded joint. The fatigue crack growth rate was measured under standard laboratory conditions using compact tensile (CT) specimens that were (i) uncharged and hydrogen pre-charged in a hydrogen pressure reactor (under 19.5 MPa and 450 °C for 21 h). The influence of fatigue frequency was assessed using frequencies of 10 Hz, 0.1 Hz, and 0.05 Hz. Additionally, two load ratios (R = 0.1 and R = 0.5) were applied to analyze their influence in the da/dN vs. ∆K curves and therefore in the fatigue crack growth rate. The embrittlement produced by the presence of internal hydrogen was clearly noticed at the beginning of the fatigue crack growth rate test (ΔK = 30 MP m ), obtaining significant higher values than without hydrogen. This effect became more notorious as the test frequency decreased and the load ratio increased. At the same time, the failure mechanism changed from ductile (striations) to brittle (hydrogen decohesion) with intergranular fracture (IG) becoming the predominant failure mechanism under the highest loads (R = 0.5). [ABSTRACT FROM AUTHOR]
- Published
- 2022
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18. Screening of Natural Products Inhibitors of SARS-CoV-2 Entry.
- Author
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González-Maldonado, Pamela, Alvarenga, Nelson, Burgos-Edwards, Alberto, Flores-Giubi, Eugenia, Barúa, Javier E., Romero-Rodríguez, Cristina, Soto-Rifo, Ricardo, Valiente-Echeverría, Fernando, Langjahr, Patricia, Cantero-González, Guadalupe, and Sotelo, Pablo H.
- Abstract
The COVID-19 pandemic has led to the search for new molecules with antiviral activity against SARS-CoV-2. The entry of the virus into the cell is one of the main targets for inhibiting SARS-CoV-2 infection. Natural products are an important source of new therapeutic alternatives against diseases. Pseudotyped viruses allow the study of SARS-CoV-2 viral entry inhibitors, and due to their simplicity, they allow the screening of a large number of antiviral candidates in Biosafety Level 2 facilities. We used pseudotyped HIV-1 with the D614G SARS-CoV-2 spike glycoprotein to test its ability to infect ACE2-expressing HEK 293T cells in the presence of diverse natural products, including 21 plant extracts, 7 essential oils, and 13 compounds from plants and fungi. The 50% cytotoxic concentration (CC
50 ) was evaluated using the resazurin method. From these analyses, we determined the inhibitory activity of the extract of Stachytarpheta cayennensis, which had a half-maximal inhibitory concentration (IC50 ) of 91.65 µg/mL, a CC50 of 693.5 µg/mL, and a selectivity index (SI) of 7.57, indicating its potential use as an inhibitor of SARS-CoV-2 entry. Moreover, our work indicates the usefulness of the pseudotyped-virus system in the screening of SARS-CoV-2 entry inhibitors. [ABSTRACT FROM AUTHOR]- Published
- 2022
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19. Mutation Distribution in the NSP4 Protein in Rotaviruses Isolated from Mexican Children with Moderate to Severe Gastroenteritis.
- Author
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González-Ochoa, Guadalupe, Menchaca, Griselda E., Hernández, Carlos E., Rodríguez, Cristina, Tamez, Reyes S., and Contreras, Juan F.
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GENETIC mutation ,ROTAVIRUSES ,GASTROENTERITIS in children ,MORPHOGENESIS in viruses ,AMINO acids ,THERAPEUTICS - Abstract
The NSP4 protein is a multifunctional protein that plays a role in the morphogenesis and pathogenesis of the rotavirus. Although NSP4 is considered an enterotoxin, the relationship between gastroenteritis severity and amino acid variations in NSP4 of the human rotavirus remains unclear. In this study, we analyzed the sequence diversity of NSP4 and the severity of gastroenteritis of children with moderate to severe gastroenteritis. The rotavirus-infected children were hospitalized before the rotavirus vaccine program in Mexico. All children had diarrhea within 1-4 days, 44 (88%) were vomiting and 35 (70%) had fevers. The severity analysis showed that 13 (26%) cases had mild gastroenteritis, 23 (46%) moderate gastroenteritis and 14 (28%) severe. NSP4 phylogenetic analysis showed three clusters within the genotype E1. Sequence analysis revealed similar mutations inside each cluster, and an uncommon variation in residue 144 was found in five of the Mexican NSP4 sequences. Most of the amino acid variations were located in the VP4 and VP6 binding site domains, with no relationship to different grades of gastroenteritis. This finding indicates that severe gastroenteritis caused by the rotavirus appears to be related to diverse viral or cellular factors instead of NSP4 activity as a unique pathogenic factor. [ABSTRACT FROM AUTHOR]
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- 2013
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20. The Positive Role of Nanometric Molybdenum–Vanadium Carbides in Mitigating Hydrogen Embrittlement in Structural Steels.
- Author
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Peral, Luis Borja, Fernández-Pariente, Inés, Colombo, Chiara, Rodríguez, Cristina, and Belzunce, Javier
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HYDROGEN embrittlement of metals ,CRACK propagation (Fracture mechanics) ,VANADIUM ,HYDROGEN atom ,EMBRITTLEMENT ,FATIGUE cracks ,STRUCTURAL steel ,FRACTURE toughness - Abstract
The influence of hydrogen on the fracture toughness and fatigue crack propagation rate of two structural steel grades, with and without vanadium, was evaluated by means of tests performed on thermally precharged samples in a hydrogen reactor at 195 bar and 450 °C for 21 h. The degradation of the mechanical properties was directly correlated with the interaction between hydrogen atoms and the steel microstructure. A LECO DH603 hydrogen analyzer was used to study the activation energies of the different microstructural trapping sites, and also to study the hydrogen eggresion kinetics at room temperature. The electrochemical hydrogen permeation technique was employed to estimate the apparent hydrogen diffusion coefficient. Under the mentioned hydrogen precharging conditions, a very high hydrogen concentration was introduced within the V-added steel (4.3 ppm). The V-added grade had stronger trapping sites and much lower apparent diffusion coefficient. Hydrogen embrittlement susceptibility increased significantly due to the presence of internal hydrogen in the V-free steel in comparison with tests carried out in the uncharged condition. However, the V-added steel grade (+0.31%V) was less sensitive to hydrogen embrittlement. This fact was ascribed to the positive effect of the precipitated nanometric (Mo,V)C to alleviate hydrogen embrittlement. Mixed nanometric (Mo,V)C might be considered to be nondiffusible hydrogen-trapping sites, in view of their strong hydrogen-trapping capability (~35 kJ/mol). Hence, mechanical behavior of the V-added grade in the presence of internal hydrogen was notably improved. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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21. NR4A3: A Key Nuclear Receptor in Vascular Biology, Cardiovascular Remodeling, and Beyond.
- Author
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Martínez-González, José, Cañes, Laia, Alonso, Judith, Ballester-Servera, Carme, Rodríguez-Sinovas, Antonio, Corrales, Irene, and Rodríguez, Cristina
- Subjects
PULMONARY arterial hypertension ,ABDOMINAL aortic aneurysms ,ORPHANS ,BIOLOGY ,ATHEROSCLEROSIS ,TISSUE remodeling - Abstract
The mechanisms committed in the activation and response of vascular and inflammatory immune cells play a major role in tissue remodeling in cardiovascular diseases (CVDs) such as atherosclerosis, pulmonary arterial hypertension, and abdominal aortic aneurysm. Cardiovascular remodeling entails interrelated cellular processes (proliferation, survival/apoptosis, inflammation, extracellular matrix (ECM) synthesis/degradation, redox homeostasis, etc.) coordinately regulated by a reduced number of transcription factors. Nuclear receptors of the subfamily 4 group A (NR4A) have recently emerged as key master genes in multiple cellular processes and vital functions of different organs, and have been involved in a variety of high-incidence human pathologies including atherosclerosis and other CVDs. This paper reviews the major findings involving NR4A3 (Neuron-derived Orphan Receptor 1, NOR-1) in the cardiovascular remodeling operating in these diseases. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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22. The Interplay of Mitochondrial Oxidative Stress and Endoplasmic Reticulum Stress in Cardiovascular Fibrosis in Obese Rats.
- Author
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Souza-Neto, Francisco V., Jiménez-González, Sara, Delgado-Valero, Beatriz, Jurado-López, Raquel, Genty, Marie, Romero-Miranda, Ana, Rodríguez, Cristina, Nieto, María Luisa, Martínez-Martínez, Ernesto, and Cachofeiro, Victoria
- Subjects
OXIDATIVE stress ,LABORATORY rats ,MITOCHONDRIA ,FIBROSIS ,ANGIOTENSIN II - Abstract
We have evaluated the role of mitochondrial oxidative stress and its association with endoplasmic reticulum (ER) stress activation in the progression of obesity-related cardiovascular fibrosis. MitoQ (200 µM) was orally administered for 7 weeks to male Wistar rats that were fed a high-fat diet (HFD, 35% fat) or a control diet (CT, 3.5% fat). Obese animals presented cardiovascular fibrosis accompanied by increased levels of extracellular matrix proteins and profibrotic mediators. These alterations were associated with ER stress activation characterized by enhanced levels (in heart and aorta vs. CT group, respectively) of immunoglobulin binding protein (BiP; 2.1-and 2.6-fold, respectively), protein disulfide-isomerase A6 (PDIA6; 1.9-fold) and CCAAT-enhancer-binding homologous protein (CHOP; 1.5- and 1.8-fold, respectively). MitoQ treatment was able to prevent (p < 0.05) these modifications at cardiac and aortic levels. MitoQ (5 nM) and the ER stress inhibitor, 4-phenyl butyric acid (4 µM), were able to block the prooxidant and profibrotic effects of angiotensin II (Ang II, 10
−6 M) in cardiac and vascular cells. Therefore, the data show a crosstalk between mitochondrial oxidative stress and ER stress activation, which mediates the development of cardiovascular fibrosis in the context of obesity and in which Ang II can play a relevant role. [ABSTRACT FROM AUTHOR]- Published
- 2021
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23. Preconditioning-Activated AKT Controls Neuronal Tolerance to Ischemia through the MDM2–p53 Pathway.
- Author
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Barrio, Emilia, Vecino, Rebeca, Sánchez-Morán, Irene, Rodríguez, Cristina, Suárez-Pindado, Alberto, Bolaños, Juan P., Almeida, Angeles, and Delgado-Esteban, Maria
- Subjects
CELL survival ,ISCHEMIC preconditioning ,ISCHEMIA ,METHYL aspartate receptors ,CELL differentiation - Abstract
One of the most important mechanisms of preconditioning-mediated neuroprotection is the attenuation of cell apoptosis, inducing brain tolerance after a subsequent injurious ischemia. In this context, the antiapoptotic PI3K/AKT signaling pathway plays a key role by regulating cell differentiation and survival. Active AKT is known to increase the expression of murine double minute-2 (MDM2), an E3-ubiquitin ligase that destabilizes p53 to promote the survival of cancer cells. In neurons, we recently showed that the MDM2–p53 interaction is potentiated by pharmacological preconditioning, based on subtoxic stimulation of NMDA glutamate receptor, which prevents ischemia-induced neuronal apoptosis. However, whether this mechanism contributes to the neuronal tolerance during ischemic preconditioning (IPC) is unknown. Here, we show that IPC induced PI3K-mediated phosphorylation of AKT at Ser
473 , which in turn phosphorylated MDM2 at Ser166 . This phosphorylation triggered the nuclear stabilization of MDM2, leading to p53 destabilization, thus preventing neuronal apoptosis upon an ischemic insult. Inhibition of the PI3K/AKT pathway with wortmannin or by AKT silencing induced the accumulation of cytosolic MDM2, abrogating IPC-induced neuroprotection. Thus, IPC enhances the activation of PI3K/AKT signaling pathway and promotes neuronal tolerance by controlling the MDM2–p53 interaction. Our findings provide a new mechanistic pathway involved in IPC-induced neuroprotection via modulation of AKT signaling, suggesting that AKT is a potential therapeutic target against ischemic injury. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
- View/download PDF
24. Biomechanical Behavior Characterization and Constitutive Models of Porcine Trabecular Tibiae.
- Author
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Quintana-Barcia, Covadonga, Rodríguez, Cristina, Álvarez, Guillermo, and Maestro, Antonio
- Subjects
- *
CANCELLOUS bone , *TIBIA , *MECHANICAL behavior of materials , *FINITE element method , *TRAUMA surgery - Abstract
Simple Summary: In surgery, when it comes to repairing a trauma injury, there are many variables that must be taken into account. For this reason, to study the possible effects of modifying any of the variables, it is necessary to approach the problem using numerical methods. In this work, the behavior of porcine trabecular bone, which is the most involved in this type of surgery, is experimentally analyzed to obtain constitutive models of behavior when using alternative techniques that simulate reality, such as the finite element method. Experimental compression tests were carried out, obtaining the mechanical properties of the material and the most suitable models were defined. The predictions of these models have been compared with the experimental results, thus choosing the most suitable one. Customizing any trauma surgery requires prior planning by surgeons. Nowadays, the use of numerical tools is increasingly needed to facilitate this planning. The success of this analysis begins with the definition of all the mechanical constitutive models of the materials implied. Our target is the trabecular bone because almost all trauma surgeries are closely related to it. This work focuses on the experimental characterization of porcine trabecular tibiae and defining its best constitutive model. Therefore, different types of compression tests were performed with tibia samples. Once the potential constitutive models were defined, stress–strain state from numerical approaches were compared with the corresponding experimental results. Experimental results from uniaxial compression tests showed than trabecular bone exhibits clear anisotropy with more stiffness and strength when it is loaded in the tibia longitudinal direction. Results from confined compression tests confirmed that the plastic behavior of trabecular bone depends on the hydrostatic and deviatoric invariants, so an alternative formulation (crushable foam volumetric (CFV)) has been proposed to describe its behavior. A new method to obtain CFV characteristic parameters has been developed and validated. Predictions of the CFV model better describe trabecular bone mechanical behavior under confined conditions. In other cases, classical plasticity formulations work better. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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25. The Inflammatory Profile of CTEPH-Derived Endothelial Cells Is a Possible Driver of Disease Progression.
- Author
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Smolders, Valérie F. E. D., Lodder, Kirsten, Rodríguez, Cristina, Tura-Ceide, Olga, Barberà, Joan Albert, Jukema, J. Wouter, Quax, Paul H. A., Goumans, Marie José, Kurakula, Kondababu, and Marsh, Leigh
- Subjects
VASCULAR cell adhesion molecule-1 ,ENDOTHELIAL cells ,DISEASE progression ,PULMONARY hypertension ,PULMONARY artery ,MONOCYTES - Abstract
Chronic thromboembolic pulmonary hypertension (CTEPH) is a form of pulmonary hypertension characterized by the presence of fibrotic intraluminal thrombi and causing obliteration of the pulmonary arteries. Although both endothelial cell (EC) dysfunction and inflammation are linked to CTEPH pathogenesis, regulation of the basal inflammatory response of ECs in CTEPH is not fully understood. Therefore, in the present study, we investigated the role of the nuclear factor (NF)-κB pro-inflammatory signaling pathway in ECs in CTEPH under basal conditions. Basal mRNA levels of interleukin (IL)-8, IL-1β, monocyte chemoattractant protein-1 (MCP-1), C-C motif chemokine ligand 5 (CCL5), and vascular cell adhesion molecule-1 (VCAM-1) were upregulated in CTEPH-ECs compared to the control cells. To assess the involvement of NF-κB signaling in basal inflammatory activation, CTEPH-ECs were incubated with the NF-κB inhibitor Bay 11-7085. The increase in pro-inflammatory cytokines was abolished when cells were incubated with the NF-κB inhibitor. To determine if NF-κB was indeed activated, we stained pulmonary endarterectomy (PEA) specimens from CTEPH patients and ECs isolated from PEA specimens for phospho-NF-κB-P65 and found that especially the vessels within the thrombus and CTEPH-ECs are positive for phospho-NF-κB-P65. In summary, we show that CTEPH-ECs have a pro-inflammatory status under basal conditions, and blocking NF-κB signaling reduces the production of inflammatory factors in CTEPH-ECs. Therefore, our results show that the increased basal pro-inflammatory status of CTEPH-ECs is, at least partially, regulated through activation of NF-κB signaling and potentially contributes to the pathophysiology and progression of CTEPH. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
26. Rolipram Prevents the Formation of Abdominal Aortic Aneurysm (AAA) in Mice: PDE4B as a Target in AAA.
- Author
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Varona, Saray, Puertas, Lídia, Galán, María, Orriols, Mar, Cañes, Laia, Aguiló, Silvia, Camacho, Mercedes, Sirvent, Marc, Andrés, Vicente, Martínez-González, José, Rodríguez, Cristina, and Zalba, Guillermo
- Subjects
ABDOMINAL aortic aneurysms ,REACTIVE oxygen species ,VASCULAR remodeling ,APOLIPOPROTEIN E ,ANGIOTENSIN II ,AORTA ,NEUTROPHILS - Abstract
Abdominal aortic aneurysm (AAA) is a common life-threatening condition characterized by exacerbated inflammation and the generation of reactive oxygen species. Pharmacological treatments to slow AAA progression or to prevent its rupture remain a challenge. Targeting phosphodiesterase 4 (PDE4) has been verified as an effective therapeutic strategy for an array of inflammatory conditions; however, no studies have assessed yet PDE4 in AAA. Here, we used angiotensin II (AngII)-infused apolipoprotein E deficient mice to study the involvement of the PDE4 subfamily in aneurysmal disease. PDE4B but not PDE4D was upregulated in inflammatory cells from both experimental and human AAA. The administration of the PDE4 selective inhibitor rolipram (3 mg/kg/day) to AngII-challenged mice (1000 ng/kg bodyweight/min) protected against AAA formation, limiting the progressive increase in the aortic diameter without affecting the blood pressure. The drug strongly attenuated the rise in vascular oxidative stress (superoxide anion) induced by AngII, and decreased the expression of inflammatory markers, as well as the recruitment of macrophages (MAC3+), lymphocytes (CD3+), and neutrophils (ELANE+) into the vessel wall. Rolipram also normalized the vascular MMP2 expression and MMP activity, preserving the elastin integrity and improving the vascular remodelling. These results point to PDE4B as a new therapeutic target for AAA. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
27. Cellulose and Graphene Based Polyurethane Nanocomposites for FDM 3D Printing: Filament Properties and Printability.
- Author
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Larraza, Izaskun, Vadillo, Julen, Calvo-Correas, Tamara, Tejado, Alvaro, Olza, Sheila, Peña-Rodríguez, Cristina, Arbelaiz, Aitor, Eceiza, Arantxa, and Kéki, Sándor
- Subjects
THREE-dimensional printing ,FUSED deposition modeling ,FIBERS ,NANOCOMPOSITE materials ,POLYURETHANES ,CELLULOSE fibers ,CELLULOSE - Abstract
3D printing has exponentially grown in popularity due to the personalization of each printed part it offers, making it extremely beneficial for the very demanding biomedical industry. This technique has been extensively developed and optimized and the advances that now reside in the development of new materials suitable for 3D printing, which may open the door to new applications. Fused deposition modeling (FDM) is the most commonly used 3D printing technique. However, filaments suitable for FDM must meet certain criteria for a successful printing process and thus the optimization of their properties in often necessary. The aim of this work was to prepare a flexible and printable polyurethane filament parting from a biocompatible waterborne polyurethane, which shows potential for biomedical applications. In order to improve filament properties and printability, cellulose nanofibers and graphene were employed to prepare polyurethane based nanocomposites. Prepared nanocomposite filaments showed altered properties which directly impacted their printability. Graphene containing nanocomposites presented sound enough thermal and mechanical properties for a good printing process. Moreover, these filaments were employed in FDM to obtained 3D printed parts, which showed good shape fidelity. Properties exhibited by polyurethane and graphene filaments show potential to be used in biomedical applications. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
28. Mindfulness in Sexual Activity, Sexual Satisfaction and Erotic Fantasies in a Non-Clinical Sample.
- Author
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Sánchez-Sánchez, Laura C., Rodríguez, María Fernanda Valderrama, García-Montes, José Manuel, Petisco-Rodríguez, Cristina, Fernández-García, Rubén, and Tchounwou, Paul B.
- Published
- 2021
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29. Relationships between Mindfulness, Purpose in Life, Happiness, Anxiety, and Depression: Testing a Mediation Model in a Sample of Women.
- Author
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Crego, Antonio, Yela, José Ramón, Gómez-Martínez, María Ángeles, Riesco-Matías, Pablo, and Petisco-Rodríguez, Cristina
- Published
- 2021
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- View/download PDF
30. Disordered Eating Attitudes, Anxiety, Self-Esteem and Perfectionism in Young Athletes and Non-Athletes.
- Author
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Petisco-Rodríguez, Cristina, Sánchez-Sánchez, Laura C., Fernández-García, Rubén, Sánchez-Sánchez, Javier, and García-Montes, José Manuel
- Published
- 2020
- Full Text
- View/download PDF
31. Measuring What Is Not Seen—Transparency and Good Governance Nonprofit Indicators to Overcome the Limitations of Accounting Models.
- Author
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Moreno-Albarracín, Antonio Luis, Licerán-Gutierrez, Ana, Ortega-Rodríguez, Cristina, Labella, Álvaro, and Rodríguez, Rosa M.
- Abstract
One of the most complex challenges currently faced by non-profit organizations (NPOs) is demonstrating that they manage resources with the highest levels of efficiency and excellence, and do not deviate from the accomplishment of their mission. Transparency and good governance are highly valuable issues for the survival of these organizations. However, empirical studies and models to measure these concepts are scarce and lack consensus. The objective of this article is to develop a uniform procedure for measuring the levels of transparency and good governance in NPOs, validated by experts, that integrates the most important contributions. The main proposals are supported by lists of indicators whose compliance they try to verify. Finally, we considered the experts' preferences to obtain the indicator weights by means of the Best–Worst Method and Minimum Cost Consensus model. The result of our work is the development of a list of indicators, which integrates the existing battery of Spanish indicators. We contribute, with this work, to improving the credibility of the third sector from the perspective of donors, users, public administrations, and society. This is an essential issue for the survival of these NPOs. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
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32. Deletion or Inhibition of NOD1 Favors Plaque Stability and Attenuates Atherothrombosis in Advanced Atherogenesis.
- Author
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González-Ramos, Silvia, Fernández-García, Victoria, Recalde, Miriam, Rodríguez, Cristina, Martínez-González, José, Andrés, Vicente, Martín-Sanz, Paloma, and Boscá, Lisardo
- Subjects
ATHEROSCLEROTIC plaque ,MACROPHAGES ,MUSCLE cells ,SINUS of valsalva ,AMYLOID plaque ,ACUTE coronary syndrome ,NATURAL immunity ,AORTA - Abstract
Atherothrombosis, the main cause of acute coronary syndromes (ACS), is characterized by the rupture of the atherosclerotic plaque followed by the formation of thrombi. Fatal plaque rupture sites show large necrotic cores combined with high levels of inflammation and thin layers of collagen. Plaque necrosis due to the death of macrophages and smooth muscle cells (SMCs) remains critical in the process. To determine the contribution of the innate immunity receptor NOD1 to the stability of atherosclerotic plaque, Apoe
−/− and Apoe−/− Nod1−/− atherosclerosis prone mice were placed on a high-fat diet for 16 weeks to assess post-mortem advanced atherosclerosis in the aortic sinus. The proliferation and apoptosis activity were analyzed, as well as the foam cell formation capacity in these lesions and in primary cultures of macrophages and vascular SMCs obtained from both groups of mice. Our results reinforce the preeminent role for NOD1 in human atherosclerosis. Advanced plaque analysis in the Apoe−/− atherosclerosis model suggests that NOD1 deficiency may decrease the risk of atherothrombosis by decreasing leukocyte infiltration and reducing macrophage apoptosis. Furthermore, Nod1−/− SMCs exhibit higher proliferation rates and decreased apoptotic activity, contributing to thicker fibrous caps with reduced content of pro-thrombotic collagen. These findings demonstrate a direct link between NOD1 and plaque vulnerability through effects on both macrophages and SMCs, suggesting promising insights for early detection of biomarkers for treating patients before ACS occurs. [ABSTRACT FROM AUTHOR]- Published
- 2020
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- View/download PDF
33. Transparency as a Key Element in Accountability in Non-Profit Organizations: A Systematic Literature Review.
- Author
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Ortega-Rodríguez, Cristina, Licerán-Gutiérrez, Ana, and Moreno-Albarracín, Antonio Luis
- Abstract
The purpose of this article is to present a framework to understand transparency in the third sector and then to explore the main research streams regarding the disclosure of information and accountability by conducting a systematic literature review on the antecedents and dimensions of the transparency of information disclosed by non-profit organizations (NPOs). The essential questions of this work are addressed from an international perspective. In particular, we explore three research questions: (1) why should NPOs disclose transparent information to stakeholders? (2) Why do not all NPOs disclose transparent information? (3) What means do NPOs use to disclose transparent information? Our results highlight the lack of transparency legislation in the third sector, leading NPOs to adopt the voluntary disclosure of information policies to improve the perceived credibility of these entities by their stakeholders. In conclusion, our study highlights the importance of developing a systematic body of knowledge regarding the situation of transparent, voluntary information disclosure in the sector. Moreover, the Sustainable Development Goals promulgated by the United Nations place a high value on transparency for the accountability of institutions. To achieve sustainable development, both peace and the governance of institutions are needed. Reducing corruption and promoting transparency are among the goals which should be reached to achieve social sustainability. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
34. The Role of Lysyl Oxidase Enzymes in Cardiac Function and Remodeling.
- Author
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Rodríguez, Cristina and Martínez-González, José
- Subjects
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LYSYL oxidase , *ENZYMES , *TISSUE mechanics , *HEART cells , *TISSUE remodeling - Abstract
Lysyl oxidase (LOX) proteins comprise a family of five copper-dependent enzymes (LOX and four LOX-like isoenzymes (LOXL1–4)) critical for extracellular matrix (ECM) homeostasis and remodeling. The primary role of LOX enzymes is to oxidize lysyl and hydroxylysyl residues from collagen and elastin chains into highly reactive aldehydes, which spontaneously react with surrounding amino groups and other aldehydes to form inter- and intra-catenary covalent cross-linkages. Therefore, they are essential for the synthesis of a mature ECM and assure matrix integrity. ECM modulates cellular phenotype and function, and strikingly influences the mechanical properties of tissues. This explains the critical role of these enzymes in tissue homeostasis, and in tissue repair and remodeling. Cardiac ECM is mainly composed of fibrillar collagens which form a complex network that provides structural and biochemical support to cardiac cells and regulates cell signaling pathways. It is now becoming apparent that cardiac performance is affected by the structure and composition of the ECM and that any disturbance of the ECM contributes to cardiac disease progression. This review article compiles the major findings on the contribution of the LOX family to the development and progression of myocardial disorders. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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35. Opposite Effects of Moderate and Extreme Cx43 Deficiency in Conditional Cx43-Deficient Mice on Angiotensin II-Induced Cardiac Fibrosis.
- Author
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Valls-Lacalle, Laura, Negre-Pujol, Corall, Rodríguez, Cristina, Varona, Saray, Valera-Cañellas, Antoni, Consegal, Marta, Martínez-González, Jose, and Rodríguez-Sinovas, Antonio
- Subjects
HEART fibrosis ,CONNEXIN 43 ,ANGIOTENSINS ,MYOFIBROBLASTS ,MICE ,ANGIOTENSIN II ,COLLAGEN - Abstract
Connexin 43 (Cx43) is essential for cardiac electrical coupling, but its effects on myocardial fibrosis is controversial. Here, we analyzed the role of Cx43 in myocardial fibrosis caused by angiotensin II (AngII) using Cx43
fl/fl and Cx43Cre-ER(T)/fl inducible knock-out (Cx43 content: 50%) mice treated with vehicle or 4-hydroxytamoxifen (4-OHT) to induce a Cre-ER(T)-mediated global deletion of the Cx43 floxed allele. Myocardial collagen content was enhanced by AngII in all groups (n = 8–10/group, p < 0.05). However, animals with partial Cx43 deficiency (vehicle-treated Cx43Cre-ER(T)/fl ) had a significantly higher AngII-induced collagen accumulation that reverted when treated with 4-OHT, which abolished Cx43 expression. The exaggerated fibrotic response to AngII in partially deficient Cx43Cre-ER(T)/fl mice was associated with enhanced p38 MAPK activation and was not evident in Cx43 heterozygous (Cx43+/- ) mice. In contrast, normalization of interstitial collagen in 4-OHT-treated Cx43Cre-ER(T)/fl animals correlated with enhanced MMP-9 activity, IL-6 and NOX2 mRNA expression, and macrophage content, and with reduced α-SMA and SM22α in isolated fibroblasts. In conclusion, our data demonstrates an exaggerated, p38 MAPK-dependent, fibrotic response to AngII in partially deficient Cx43Cre-ER(T)/fl mice, and a paradoxical normalization of collagen deposition in animals with an almost complete Cx43 ablation, an effect associated with increased MMP-9 activity and inflammatory response and reduced fibroblasts differentiation. [ABSTRACT FROM AUTHOR]- Published
- 2019
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- View/download PDF
36. Emerging Roles of Lysyl Oxidases in the Cardiovascular System: New Concepts and Therapeutic Challenges.
- Author
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Martínez-González, José, Varona, Saray, Cañes, Laia, Galán, María, Briones, Ana M, Cachofeiro, Victoria, and Rodríguez, Cristina
- Subjects
LYSYL oxidase ,CARDIOVASCULAR system ,ENDOTHELIUM diseases ,HYPERTENSION ,CARDIOVASCULAR diseases ,DISSECTING aneurysms ,OXIDASES ,COLLAGEN - Abstract
Lysyl oxidases (LOX and LOX-likes (LOXLs) isoenzymes) belong to a family of copper-dependent enzymes classically involved in the covalent cross-linking of collagen and elastin, a pivotal process that ensures extracellular matrix (ECM) stability and provides the tensile and elastic characteristics of connective tissues. Besides this structural role, in the last years, novel biological properties have been attributed to these enzymes, which can critically influence cardiovascular function. LOX and LOXLs control cell proliferation, migration, adhesion, differentiation, oxidative stress, and transcriptional regulation and, thereby, their dysregulation has been linked to a myriad of cardiovascular pathologies. Lysyl oxidase could modulate virtually all stages of the atherosclerotic process, from endothelial dysfunction and plaque progression to calcification and rupture of advanced and complicated plaques, and contributes to vascular stiffness in hypertension. The alteration of LOX/LOXLs expression underlies the development of other vascular pathologies characterized by a destructive remodeling of the ECM, such as aneurysm and artery dissections, and contributes to the adverse myocardial remodeling and dysfunction in hypertension, myocardial infarction, and obesity. This review examines the most recent advances in the study of LOX and LOXLs biology and their pathophysiological role in cardiovascular diseases with special emphasis on their potential as therapeutic targets. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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37. Proteomics, Holm Oak (Quercus ilex L.) and Other Recalcitrant and Orphan Forest Tree Species: How do They See Each Other?
- Author
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Rey, María-Dolores, Castillejo, María Ángeles, Sánchez-Lucas, Rosa, Guerrero-Sanchez, Victor M., López-Hidalgo, Cristina, Romero-Rodríguez, Cristina, Valero-Galván, José, Sghaier-Hammami, Besma, Simova-Stoilova, Lyudmila, Echevarría-Zomeño, Sira, Jorge, Inmaculada, Gómez-Gálvez, Isabel, Papa, María Eugenia, Carvalho, Kamilla, Rodríguez de Francisco, Luis E., Maldonado-Alconada, Ana María, Valledor, Luis, and Jorrín-Novo, Jesús V.
- Subjects
PROTEOMICS ,OAK ,PINE ,EUCALYPTUS ,ABIOTIC stress - Abstract
Proteomics has had a big impact on plant biology, considered as a valuable tool for several forest species, such as Quercus, Pines, Poplars, and Eucalyptus. This review assesses the potential and limitations of the proteomics approaches and is focused on Quercus ilex as a model species and other forest tree species. Proteomics has been used with Q. ilex since 2003 with the main aim of examining natural variability, developmental processes, and responses to biotic and abiotic stresses as in other species of the genus Quercus or Pinus. As with the progress in techniques in proteomics in other plant species, the research in Q. ilex moved from 2-DE based strategy to the latest gel-free shotgun workflows. Experimental design, protein extraction, mass spectrometric analysis, confidence levels of qualitative and quantitative proteomics data, and their interpretation are a true challenge with relation to forest tree species due to their extreme orphan and recalcitrant (non-orthodox) nature. Implementing a systems biology approach, it is time to validate proteomics data using complementary techniques and integrate it with the -omics and classical approaches. The full potential of the protein field in plant research is quite far from being entirely exploited. However, despite the methodological limitations present in proteomics, there is no doubt that this discipline has contributed to deeper knowledge of plant biology and, currently, is increasingly employed for translational purposes. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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38. Population Genetic Diversity of Quercus ilex subsp. ballota (Desf.) Samp. Reveals Divergence in Recent and Evolutionary Migration Rates in the Spanish Dehesas.
- Author
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Fernández i Marti, Angel, Romero-Rodríguez, Cristina, Navarro-Cerrillo, Rafael M., Abril, Nieves, Jorrín-Novo, Jesús V., and Dodd, Richard S.
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HOLM oak ,PLANT population genetics ,PLANT migration ,GENE flow in plants ,NUCLEAR DNA - Abstract
The Spanish dehesas have been severely affected by human activities that date to the prehistoric period and have suffered accelerated decline since the 1980s. Holm oak (
Quercus ilex subsp.ballota (Desf.) Samp.) is a key component of this system, and its acorns provide an important food source for wildlife and domesticated livestock. Our earlier work showed structured variation in acorn morphology and biochemistry. Here, we used chloroplast and nuclear microsatellites to detect genetic structure among populations ofQ. ilex from the major biogeographic regions of Andalusia. We found high levels of spatial differentiation with chloroplast DNA indicating little seed dispersal among populations. Spatial differentiation was weaker for nuclear DNA, presumably as a result of more widespread pollen dispersal and its larger effective population size. The Baetic Cordillera (Cádiz) population consistently appeared well separated from populations of the northern Sierra Morena, suggesting that the Guadalquivir Valley has played an important role in determining population divergence. This may be, in part, evolutionary, as suggested by chloroplast DNA, and, in part, a result of human-induced population isolation, asQ. ilex has been removed from the Guadalquivir Valley. Evolutionary gene flow rates were greater than contemporary rates, which were limited to unidirectional gene flow from Córdoba to other populations in the Sierra Morena and, surprisingly, to the southern population at Almería. The inconsistency between evolutionary and recent migration rates suggests an effect of anthropogenic activity over the last few generations ofQ. ilex . [ABSTRACT FROM AUTHOR]- Published
- 2018
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39. Underreporting of Cases in the COVID-19 Outbreak of Borriana (Spain) during Mass Gathering Events in March 2020: A Cross-Sectional Study.
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Domènech-Montoliu S, Pac-Sa MR, Sala-Trull D, Del Rio-González A, Sanchéz-Urbano M, Satorres-Martinez P, Blasco-Gari R, Casanova-Suarez J, Gil-Fortuño M, López-Diago L, Notari-Rodríguez C, Pérez-Olaso Ó, Romeu-Garcia MA, Ruiz-Puig R, Aleixandre-Gorriz I, Domènech-León C, and Arnedo-Pena A
- Abstract
Determining the number of cases of an epidemic is the first function of epidemiological surveillance. An important underreporting of cases was observed in many locations during the first wave of the COVID-19 pandemic. To estimate this underreporting in the COVID-19 outbreak of Borriana (Valencia Community, Spain) in March 2020, a cross-sectional study was performed in June 2020 querying the public health register. Logistic regression models were used. Of a total of 468 symptomatic COVID-19 cases diagnosed in the outbreak through anti-SARS-CoV-2 serology, 36 cases were reported (7.7%), resulting in an underreporting proportion of 92.3% (95% confidence interval [CI], 89.5-94.6%), with 13 unreported cases for every reported case. Only positive SARS-CoV-2 polymerase chain reaction cases were predominantly reported due to a limited testing capacity and following a national protocol. Significant factors associated with underreporting included no medical assistance for COVID-19 disease, with an adjusted odds ratio [aOR] of 10.83 (95% CI 2.49-47.11); no chronic illness, aOR = 2.81 (95% CI 1.28-6.17); middle and lower social classes, aOR = 3.12 (95% CI 1.42-6.85); younger age, aOR = 0.97 (95% CI 0.94-0.99); and a shorter duration of illness, aOR = 0.98 (95% CI 0.97-0.99). To improve the surveillance of future epidemics, new approaches are recommended.
- Published
- 2024
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40. Spanish HCMV Seroprevalence in the 21st Century.
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Álvarez-Heredia P, Reina-Alfonso I, Domínguez-Del-Castillo JJ, Hassouneh F, Gutiérrez-González C, Batista-Duharte A, Pérez AB, Sarramea F, Jaén-Moreno MJ, Camacho-Rodríguez C, Tarazona R, Solana R, Molina J, and Pera A
- Subjects
- Adult, Humans, Infant, Newborn, Female, Cytomegalovirus, Seroepidemiologic Studies, Tissue Donors, Antibodies, Viral, Immunoglobulin G, Cytomegalovirus Infections, Cardiovascular Diseases
- Abstract
Human cytomegalovirus (HCMV) is linked to age-related diseases like cardiovascular disease, neurodegenerative conditions, and cancer. It can also cause congenital defects and severe illness in immunocompromised individuals. Accurate HCMV seroprevalence assessment is essential for public health planning and identifying at-risk individuals. This is the first HCMV seroprevalence study conducted in the general Spanish adult population in 30 years. We studied HCMV seroprevalence and HCMV IgG antibody titres in healthy adult donors (HDs) and HCMV-related disease patients from 2010 to 2013 and 2020 to 2023, categorized by sex and age. We compared our data with 1993 and 1999 studies in Spain. The current HCMV seroprevalence among HDs in Spain is 73.48%. In women of childbearing age, HCMV seroprevalence has increased 1.4-fold in the last decade. HCMV-seropositive individuals comprise 89.83% of CVD patients, 69% of SMI patients, and 70.37% of COVID-19 patients. No differences in HCMV seroprevalence or HCMV IgG antibody titres were observed between patients and HDs. A significant reduction in Spanish HCMV seroprevalence among HDs was observed in 1993. However, women of childbearing age have shown an upturn in the last decade that may denote a health risk in newborns and a change in HCMV seroprevalence trends.
- Published
- 2023
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41. ABO Blood Groups and Incidence of COVID-19 in the Mass Gathering Events in Borriana (Spain), March 2020: A Retrospective Cohort Study.
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Domènech-Montoliu S, Puig-Barberà J, Guerra-Murcia O, Pac-Sa MR, Orrico-Sanchéz A, Gómez-Lanas L, Sala-Trull D, Domènech-Leon C, Del Rio-González A, Sánchez-Urbano M, Satorres-Martinez P, Latorre-Poveda M, Ferrando-Rubert S, Aparisi-Esteve L, Badenes-Marques G, Blasco-Gari R, Casanova-Suarez J, Fontal-Carcel M, Gil-Fortuño M, Hernández-Pérez N, Jovani-Sales D, López-Diago L, Notari-Rodríguez C, Pérez-Olaso O, Romeu-Garcia MA, Ruíz-Puig R, and Arnedo-Pena A
- Abstract
Our objective was to estimate the incidence of COVID-19 and the ABO blood Groups in the mass-gathering events (MGEs) during the Falles Festival in Borriana (Spain) from 6-10 March 2020. We conducted a population-based retrospective cohort study and measured anti-SARS-CoV-2 antibodies and the ABO of participants. We performed laboratory COVID-19 tests and obtained the ABO in 775 subjects (72.8% of the original exposed cohort): O-group (45.2%), A-group (43.1%), B-group (8.5%) and AB-group (3.4%). Adjusted for confounding factors, including COVID-19 exposure during the MGEs, attack rates of COVID-19 for each ABO group were 55.4%, 59.6%, 60.2%, and 63.7%. The adjusted relative risks were for O-group 0.93 (95% Confidence Interval [CI] 0.83-1.04), for A-group 1.06 (95% CI 0.94-1.18), for B-group 1.04 (95%CI 0.88-1.24), and for AB-group 1.11 (95% CI 0.81-1.51) with no significant differences. Conclusions: Our results suggest no effect of ABO on COVID-19 incidence. We observed weak but not significant protection of the O-group and not a significantly greater infection risk for the remaining groups compared with the O-group. More studies are needed to resolve the controversies regarding the association between ABO and COVID-19.
- Published
- 2023
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42. Complications Post-COVID-19 and Risk Factors among Patients after Six Months of a SARS-CoV-2 Infection: A Population-Based Prospective Cohort Study.
- Author
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Domènech-Montoliu S, Puig-Barberà J, Pac-Sa MR, Vidal-Utrillas P, Latorre-Poveda M, Del Rio-González A, Ferrando-Rubert S, Ferrer-Abad G, Sánchez-Urbano M, Aparisi-Esteve L, Badenes-Marques G, Cervera-Ferrer B, Clerig-Arnau U, Dols-Bernad C, Fontal-Carcel M, Gomez-Lanas L, Jovani-Sales D, León-Domingo MC, Llopico-Vilanova MD, Moros-Blasco M, Notari-Rodríguez C, Ruíz-Puig R, Valls-López S, and Arnedo-Pena A
- Abstract
In October 2020, we conducted a population-based prospective cohort study to determine post-COVID-19 complications, recovery, return to usual health, and associated risk factors in 536 cases of COVID-19 outbreak in Borriana (Spain) by administering an epidemiological questionnaire via phone interviews. A total of 484 patients participated (90.3%), age mean 37.2 ± 17.1 years, and 301 females (62.2%). Mild illness was the most common COVID-19 manifestation. After six months, 160 patients (33.1%) suffered at least one complication post-COVID-19, and 47 (29.4%) of them sought medical assistance. The most frequent persistent symptoms were hair loss, fatigue, loss of smell or taste, and headache. Risk factors associated with a complication were female sex (adjusted relative risk, [aRR] = 1.93 95% confidence interval [CI] 1.41-2.65), age 35 years and above (aRR = 1.50 95% CI 1.14-1.99), B blood group (aRR = 1.51 95% CI 1.04-2.16), current smoker (RR = 1.61 95% CI 1.02-2.54), and at least a COVID-19 exposure (aRR = 2.13 95% CI 1.11-4.09). Male sex, age younger than 35 years, and low COVID-19 exposures were associated with better recovery and return to usual health. A third of patients presented persistent symptoms compatible with the long-COVID-19 syndrome. In conclusion, an active medical follow-up of post-COVID-19 patients must be implemented.
- Published
- 2022
- Full Text
- View/download PDF
43. ABO Blood Groups and the Incidence of Complications in COVID-19 Patients: A Population-Based Prospective Cohort Study.
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Domènech-Montoliu S, Puig-Barberà J, Pac-Sa MR, Vidal-Utrillas P, Latorre-Poveda M, Rio-González AD, Ferrando-Rubert S, Ferrer-Abad G, Sánchez-Urbano M, Aparisi-Esteve L, Badenes-Marques G, Cervera-Ferrer B, Clerig-Arnau U, Dols-Bernad C, Fontal-Carcel M, Gomez-Lanas L, Jovani-Sales D, León-Domingo MC, Llopico-Vilanova MD, Moros-Blasco M, Notari-Rodríguez C, Ruíz-Puig R, Valls-López S, and Arnedo-Pena A
- Subjects
- Adult, Cohort Studies, Female, Humans, Incidence, Middle Aged, Prospective Studies, SARS-CoV-2, Young Adult, ABO Blood-Group System, COVID-19
- Abstract
After a COVID-19 outbreak in the Falles festival of Borriana (Spain) during March 2020, a cohort of patients were followed until October 2020 to estimate complications post-COVID-19, considering ABO blood groups (ABO). From 536 laboratory-confirmed cases, 483 completed the study (90.1%) carried by the Public Health Center of Castelló and the Emergency and Microbiology and Clinical Analysis of Hospital de la Plana Vila-real. The study included ABO determination and telephone interviews of patients. The participants had a mean age of 37.2 ± 17.1 years, 300 females (62.1%). ABO were O (41.4%), A (45.5%), B (9.1%), and AB (3.9%). We found no difference in the incidence of COVID-19 infections. A total of 159 (32.9%) patients reported one or more post-COVID-19 complications with divergent incidences after adjustment: O (32.3%), A (32.6%), B (54.1%), and AB (27.6%); B groups had more complications post-COVID-19 when compared with O group (adjusted relative risk [aRR] 95% confidence interval [CI] 1.68, 95% CI 1.24-2.27), and symptoms of fatigue (1.79, 95% CI 1.08-2.95), myalgia (2.06, 95% CI 1.10-3.84), headache (2.61, 95% CI 1.58-4.31), and disorder of vision (4.26 95% CI 1.33-13.60). In conclusion, we observed significant differences in post-COVID-19 complications by ABO, with a higher incidence in B group. Additional research is justified to confirm our results.
- Published
- 2021
- Full Text
- View/download PDF
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