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Satellite DNA (sat-DNA) was previously described as junk and selfish DNA in the cellular economy, without a clear functional role. However, during the last two decades, evidence has been accumulated about the roles of sat-DNA in different cellular functions and its probable involvement in tumorigenesis and adaptation to environmental changes. In molluscs, studies on sat-DNAs have been performed mainly on bivalve species, especially those of economic interest. Conversely, in Gastropoda (which includes about 80% of the currently described molluscs species), studies on sat-DNA have been largely neglected. In this study, we isolated and characterized a sat-DNA, here named PcH-sat, in the limpet Patella caerulea using the restriction enzyme method, particularly Hae III. Monomeric units of PcH-sat are 179 bp long, AT-rich (58.7%), and with an identity among monomers ranging from 91.6 to 99.8%. Southern blot showed that PcH-sat is conserved in P. depressa and P. ulyssiponensis , while a smeared signal of hybridization was present in the other three investigated limpets ( P. ferruginea , P. rustica and P. vulgata ). Dot blot showed that PcH-sat represents about 10% of the genome of P. caerulea , 5% of that of P. depressa , and 0.3% of that of P. ulyssiponensis . FISH showed that PcH-sat was mainly localized on pericentromeric regions of chromosome pairs 2 and 4-7 of P. caerulea (2n = 18). A database search showed that PcH-sat contains a large segment (of 118 bp) showing high identity with a homologous trait of the Nin-SINE transposable element (TE) of the patellogastropod Lottia gigantea , supporting the hypothesis that TEs are involved in the rising and tandemization processes of sat-DNAs.

The increasing focus on the development of positioning techniques reflects the growing interest in applications and services based on indoor positioning. Many applications necessitate precise indoor positioning or tracking of individuals and assets, leading to rapid growth in products based on these technologies in certain market sectors. Ultrasonic systems have already proven effective in achieving the desired positioning accuracy and refresh rates. The typical signal used in ultrasonic positioning systems for estimating the range between the target and reference points is the linear chirp. Unfortunately, it can undergo shape aberration due to the effects of acoustic diffraction when the aperture exceeds a certain limit. The extent of the aberration is influenced by the shape and size of the transducer, as well as the angle at which the transducer is observed by the receiver. This aberration also affects the shape of the cross-correlation, causing it to lose its easily detectable characteristic of a single global peak, which typically corresponds to the correct lag associated with the signal's time of arrival. In such instances, cross-correlation techniques yield results with a significantly higher error than anticipated. In fact, the correct lag no longer corresponds to the peak of the cross-correlation. In this study, an alternative technique to global peak detection is proposed, leveraging the inherent symmetry observed in the shape of the aberrated cross-correlation. The numerical simulations, performed using the academic acoustic simulation software Field II, conducted using a typical ultrasonic chirp and ultrasonic emitter, compare the classical and the proposed range techniques in a standard office room. The analysis includes the effects of acoustical reflection in the room and of the acoustic noise at different levels of power. The results demonstrate that the proposed technique enables accurate range estimation even in the presence of severe cross-correlation shape aberrations and for signal-to-noise ratio levels common in office and room environments, even in presence of typical reflections. This allows the use of emitting transducers with a much larger aperture than that allowed by the classical cross-correlation technique. Consequently, it becomes possible to have greater acoustic power available, leading to improved signal-to-noise ratio (SNR).

Psychotropic drugs and benzodiazepines are nowadays among the primary substances of abuse. This results in a large and constant release into aquatic environments where they have potentially harmful effects on non-target organisms and, eventually, human health. In the last decades, evidence has been collected on the possible interference of benzodiazepines with reproductive processes, but data are few and incomplete. In this study, the possible negative influence of delorazepam on fertilization and embryo development has been tested in Paracentrotus lividus , a key model organism in studies of reproduction and embryonic development. Sperm, eggs, or fertilized eggs have been exposed to delorazepam at three concentrations: 1 μg/L (environmentally realistic), 5 μg/L, and 10 μg/L. Results indicate that delorazepam reduces the fertilizing capacity of male and female gametes and interferes with fertilization and embryo development. Exposure causes anatomical anomalies in plutei, accelerates/delays development, and alters the presence and distribution of glycoconjugates such as N-Acetyl-glucosamine, α-linked fucose, and α-linked mannose in both morulae and plutei. These results should attract attention to the reproductive fitness of aquatic species exposed to benzodiazepines and pave the way for further investigation of the effects they may exert on human fertility. The presence of benzodiazepines in the aquatic environment raises concerns about the reproductive well-being of aquatic species. Additionally, it prompts worries regarding potential impacts on human fertility due to the excessive use of anxiolytics.

Plastic pollution is an important environmental problem, and microplastics have been shown to have harmful effects on human and animal health, affecting immune and metabolic physiological functions. Further, microplastics can interfere with commensal microorganisms and exert deleterious effects on exposure to pathogens. Here, we compared the effects of 1 µm diameter polystyrene microplastic (PSMPs) on Candida albicans infection in both in vitro and in vivo models by using HT29 cells and Galleria mellonella larvae, respectively. The results demonstrated that PSMPs could promote Candida infection in HT29 cells and larvae of G. mellonella , which show immune responses similar to vertebrates. In this study, we provide new experimental evidence for the risk to human health posed by PSMPs in conjunction with Candida infections.

Benzodiazepines, psychotropic drugs, are ubiquitous in the aquatic environment due to over-consumption and inefficient removal by sewage treatment plants. Bioaccumulation with consequent behavioral and physiological effects has been reported in many aquatic species. However, the responses are species-specific and still poorly understood. To improve the knowledge, we exposed the freshwater snail Planorbarius corneus to 1, 5, or 10 µg/L of delorazepam, the most widely consumed benzodiazepine in Italy. Conventional behavioral tests were used to assess the effects on locomotor and feeding behavior. Histological and biochemical analyses were also performed to detect possible changes in the structure and composition of the foot mucus and glands. The results show a paradoxical response with reduced feeding activity and locomotor hyperactivity. Pedal mucus was altered in texture but not in composition, becoming particularly rich in fibrous collagen-like material, and a significant change in the protein composition was highlighted in the foot. In conclusion, exposure to delorazepam induces disinhibited behavior in Planorbarius corneus , potentially increasing the risk of predation, and an increase in mucus protein production, which, together with reduced feeding activity, would severely compromise energy resources.

In vitro systems have been mainly promoted by authorities to sustain research by following the 3Rs principle, but continuously increasing amounts of evidence point out that in vivo experimentation is also of extreme relevance. Xenopus laevis , an anuran amphibian, is a significant model organism in the study of evolutionary developmental biology, toxicology, ethology, neurobiology, endocrinology, immunology and tumor biology; thanks to the recent development of genome editing, it has also acquired a relevant position in the field of genetics. For these reasons, X. laevis appears to be a powerful and alternative model to the zebrafish for environmental and biomedical studies. Its life cycle, as well as the possibility to obtain gametes from adults during the whole year and embryos by in vitro fertilization, allows experimental studies of several biological endpoints, such as gametogenesis, embryogenesis, larval growth, metamorphosis and, of course, the young and adult stages. Moreover, with respect to alternative invertebrate and even vertebrate animal models, the X. laevis genome displays a higher degree of similarity with that of mammals. Here, we have reviewed the main available literature on the use of X. laevis in the biosciences and, inspired by Feymann's revised view, "Plenty of room for biology at the bottom", suggest that X. laevis is a very useful model for all possible studies., Competing Interests: The authors declare no conflicts of interest.

The number of older people needing healthcare is a growing global phenomenon. The assistance in long-term care comprises a complex of medical, nursing, rehabilitation, and social assistance services. The cost is substantial, but technology can help reduce spending by ensuring efficient health services and improving the quality of life. Advances in artificial intelligence, wireless communication systems, and nanotechnology allow the creation of intelligent home care systems avoiding hospitalization with evident cost containment. They are capable of ensuring functions of recognition of activities, monitoring of vital functions, and tracking. However, it is essential to also have information on location in order to be able to promptly intervene in case of unforeseen events or assist people in carrying out activities in order to avoid incorrect behavior. In addition, the automatic detection of physical activities performed by human subjects is identified as human activity recognition (HAR). This work presents an overview of the positioning system as part of an integrated HAR system. Lastly, this study contains each technology's concepts, features, accuracy, advantages, and limitations. With this work, we want to highlight the relationship between HAR and the indoor positioning system (IPS), which is poorly documented in the literature.

During an infection, a single or multispecies biofilm can develop. Infections caused by non-dermatophyte molds, such as Fusarium spp. and yeasts, such as Candida spp., are particularly difficult to treat due to the formation of a mixed biofilm of the two species. Fusarium oxysporum is responsible for approximately 20% of human fusariosis, while Candida albicans is responsible for superficial mucosal and dermal infections and for disseminated bloodstream infections with a mortality rate above 40%. This study aims to investigate the interactions between C. albicans and F. oxysporum dual-species biofilm, considering variable formation conditions. Further, the ability of the WMR peptide, a modified version of myxinidin, to eradicate the mixed biofilm when used alone or in combination with fluconazole (FLC) was tested, and the efficacy of the combination of WMR and FLC at low doses was assessed, as well as its effect on the expression of some biofilm-related adhesin and hyphal regulatory genes. Finally, in order to confirm our findings in vivo and explore the synergistic effect of the two drugs, we utilized the Galleria mellonella infection model. We concluded that C. albicans negatively affects F. oxysporum growth in mixed biofilms. Combinatorial treatment by WMR and FLC significantly reduced the biomass and viability of both species in mature mixed biofilms, and these effects coincided with the reduced expression of biofilm-related genes in both fungi. Our results were confirmed in vivo since the synergistic antifungal activity of WMR and FLC increased the survival of infected larvae and reduced tissue invasion. These findings highlight the importance of drug combinations as an alternative treatment for C. albicans and F. oxysporum mixed biofilms.

Cardiovascular diseases are the leading cause of death in people with diabetes. Diabetic cardiomyopathy (DC) is an important complication of diabetes and represents a distinct subtype of heart failure that occurs in absence of cardiovascular diseases. Chronic hyperglycemia and hyperinsulinemia along with insulin resistance and inflammatory milieu are the main mechanisms involved in the pathophysiology of DC. Changes in lifestyle favoring healthy dietary patterns and physical activity, combined with more innovative anti-diabetes therapies, are the current treatment strategies to safeguard the cardiovascular system. This review aims at providing an updated comprehensive overview of clinical, pathogenetic, and molecular aspects of DC, with a focus on the effects of anti-hyperglycemic drugs on the prevention of pump dysfunction and consequently on cardiovascular health in type 2 diabetes.

Tattooing is a technique that introduces colored substances under the skin in order to color it permanently. Decomposition products of tattoo pigments produce numerous damages for the skin and other organs. We studied the effects of a commercial red ink tattoo, PR170, on Xenopus laevis embryos and Daphnia magna nauplii using concentrations of 10, 20, and 40 mg/L. For Xenopus , we applied the FETAX protocol analyzing survival, malformations, growth, heart rate, and the expression of genes involved in the development. In D. magna , we evaluated the toxicity with an immobilization test. Moreover, we investigated the production of ROS, antioxidant enzymes, and the expression of the ATP-binding cassette in both models. Our results indicate that PR170 pigment has nanoparticle dimensions, modifies the survival and the ATP-binding cassette activity, and induces oxidative stress that probably produces the observed effects in both models. Deformed embryos were observed in Xenopus , probably due to the modification of expression of genes involved in development. The expression of pro-inflammatory cytokines was also modified in this amphibian. We think that these effects are due to the accumulation of PR170 and, in particular, to the presence of the azoic group in the chemical structure of this pigment. Further studies needed to better understand the effects of commercial tattoo inks.

Measuring the distance between two points has multiple uses. Position can be geometrically calculated from multiple measurements of the distance between reference points and moving sensors. Distance measurement can be done by measuring the time of flight of an ultrasonic signal traveling from an emitter to receiving sensors. However, this requires close synchronization between the emitter and the sensors. This synchronization is usually done using a radio or optical channel, which requires additional hardware and power to operate. On the other hand, for many applications of great interest, low-cost, small, and lightweight sensors with very small batteries are required. Here, an innovative technique to measure the distance between emitter and receiver by using ultrasonic signals in air is proposed. In fact, the amount of the signal attenuation in air depends on the frequency content of the signal itself. The attenuation level that the signal undergoes at different frequencies provides information on the distance between emitter and receiver without the need for any synchronization between them. A mathematical relationship here proposed allows for estimating the distance between emitter and receiver starting from the measurement of the frequency dependent attenuation along the traveled path. The level of attenuation in the air is measured online along the operation of the proposed technique. The simulations showed that the range accuracy increases with the decrease of the ultrasonic transducer diameter. In particular, with a diameter of 0.5 mm, an error of less than ± 2.7 cm (average value 1.1 cm) is reached along two plane sections of the typical room of the office considered (4 × 4 × 3 m 3 ).

Extending the lifetime of power light-emitting diodes (LEDs) is achievable if proper control methods are implemented to reduce the side effects of an excessive junction temperature, T J . The accuracy of state-of-the-art LED junction temperature monitoring techniques is negatively affected by several factors, such as the use of external sensors, calibration procedures, devices aging, and technological diversity among samples with the same part number. Here, a novel method is proposed, indeed based on the well-known technique consisting in tracking the LED forward voltage drop when a fixed forward current is imposed but exploiting the voltage variation with respect to room temperature. This method, which limits the effects of sample heterogeneity, is applied to a set of ten commercial devices. The method led to an effective reduction of the measurement error, which was below 1 °C.

Increasing efforts toward the development of positioning techniques testify the growing interest for indoor position-based applications and services. Many applications require accurate indoor positioning or tracking of people and assets, and some market sectors are starting a rapid growth of products based on these technologies. Ultrasonic systems have already been demonstrating their effectiveness and to possess the desired positioning accuracy and refresh rates. In this work, it is shown that a typical signal used in ultrasonic positioning systems to estimate the range between the target and reference points-namely, the linear chirp-due to the effects of acoustic diffraction, in some cases, undergoes a shape aberration, depending on the shape and size of the transducer and on the angle under which the transducer is seen by the receiver. In the presence of such signal shape aberrations, even one of the most robust ranging techniques, which is based on cross-correlation, provides results affected by a much greater error than expected. Numerical simulations are carried out for a typical ultrasonic chirp, ultrasonic emitter, and range technique based on cross-correlation and for a typical office room, obtained using the academic acoustic simulation software Field II. Spatial distributions of the ranging error are provided, clearly showing the favorable low error regions. The work demonstrates that particular attention must be paid to the design of the acoustic section of the ultrasonic positioning systems, considering both the shape and size of the ultrasonic emitters and the shape of the acoustic signal used.

The growing interest for indoor position-based applications and services, as well as ubiquitous computing and location aware information, have led to increasing efforts toward the development of positioning techniques. Many applications require accurate positioning or tracking of people and assets inside buildings, and some market sectors are waiting for such technologies for starting a fast growth. Ultrasonic systems have already been shown to possess the desired positioning accuracy and refresh rate. However, they still require accurate synchronization between ultrasound emitters and receivers to work properly. Usually, synchronization is carried out through radio frequency (RF) signals, adding system complexity and raising the cost. In this work, this limit is overcome by introducing a novel self-synchronizing indoor positioning technique. Ultrasonic signals travel from emitters placed at fixed reference positions to any number of mobile devices (MD). The travelled distance is computed from the time of flight (TOF), which requires in turn synchronism between emitter and receiver. It is shown that this synchronism can be indirectly estimated from the time difference of arrival (TDOA) of the ultrasonic signals. The obtained positioning information is private, in the sense that the positioning infrastructure is not aware of the number or identity of the MDs that use it. Computer simulations and experimental results obtained in a typical office room are provided., Competing Interests: The authors declare no conflict of interest.

The problem of drug resistance is very worrying and ever increasing. Resistance is due not only to the reckless use of antibiotics but also to the fact that pathogens are able to adapt to different conditions and develop self-defense mechanisms such as living in biofilms; altogether these issues make the search for alternative drugs a real challenge. Antimicrobial peptides appear as promising alternatives but they have disadvantages that do not make them easily applicable in the medical field; thus many researches look for solutions to overcome the disadvantages and ensure that the advantages can be exploited. This review describes the biofilm characteristics and identifies the key features that antimicrobial peptides should have. Recalcitrant bacterial infections caused by the most obstinate bacterial species should be treated with a strategy to combine conventional peptides functionalized with nano-tools. This approach could effectively disrupt high density infections caused by biofilms. Moreover, the importance of using in vivo non mammalian models for biofilm studies is described. In particular, here we analyze the use of amphibians as a model to substitute the rodent model.

chlorpyrifos (CPF) is an organophosphate insecticide used to control pests on a variety of food and feed crops. In mammals, maternal exposure to CPF has been reported to induce cerebral cortex thinning, alteration of long-term brain cognitive function, and Parkinson-like symptoms, but the mechanisms of these processes are not fully understood. In this study, we aimed to gain a deeper understanding of the alterations induced in the brains of mice chronically exposed to CPF by dietary intake. For our purpose, we analysed F1 offspring (sacrificed at 3 and 8 months) of Mus musculus , treated in utero and postnatally with 3 different doses of CPF (0.1-1-10 mg/kg/day). Using RT² Profiler PCR Arrays, we evaluated the alterations in the expression of 84 genes associated with neurodegenerative diseases. In the brains of exposed mice, we evidenced a clear dose-response relationship for AChE inhibition and alterations of gene expression. Some of the genes that were steadily down-regulated, such as Pink1 , Park 2 , Sv2b , Gabbr2 , Sept5 and Atxn2 , were directly related to Parkinson's onset. Our experimental results shed light on the possibility that long-term CPF exposure may exert membrane signalling alterations which make brain cells more susceptible to develop neurodegenerative diseases.

Nano-based products are widespread in several sectors, including textiles, medical-products, cosmetics, paints and plastics. Nanosafety and safe-by-design are driving nanoparticle (NP) production and applications through NP functionalization (@NPs). Indeed, @NPs frequently present biological effects that differ from the parent material. This paper reviews the impact of quantum dots (QDs), gold nanoparticles (AuNPs), and polystyrene-cored NPs (PSNPs), evidencing the role of NP functionalization in toxicity definition. Key biological models were taken into consideration for NP evaluation: Saccharomyces cerevisiae , fresh- (F) and saltwater (S) microalgae ( Raphidocelis subcapitata (F), Scenedesmus obliquus (F) and Chlorella spp. (F), and Phaeodactylum tricornutum (S)), Daphnia magna , and Xenopus laevis . QDs are quite widespread in technological devices, and they are known to induce genotoxicity and oxidative stress that can drastically change according to the coating employed. For example, AuNPs are frequently functionalized with antimicrobial peptides, which is shown to both increase their activity and decrease the relative environmental toxicity. P-NPs are frequently coated with NH₂ - for cationic and COOH - for anionic surfaces, but when positively charged toxicity effects can be observed. Careful assessment of functionalized and non-functionalized NPs is compulsory to also understand their potential direct and indirect effects when the coating is removed or degraded., Competing Interests: The authors declare no conflict of interest.