1. The Chemopreventive Effects of Polyphenols and Coffee, Based upon a DMBA Mouse Model with microRNA and mTOR Gene Expression Biomarkers.
- Author
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Molnar, Richard, Szabo, Laszlo, Tomesz, Andras, Deutsch, Arpad, Darago, Richard, Raposa, Bence L., Ghodratollah, Nowrasteh, Varjas, Timea, Nemeth, Balazs, Orsos, Zsuzsanna, Pozsgai, Eva, Szentpeteri, Jozsef L., Budan, Ferenc, and Kiss, Istvan
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GENE expression ,ONCOGENES ,MITOGEN-activated protein kinases ,LABORATORY mice ,POLYPHENOLS ,ANIMAL disease models ,PROANTHOCYANIDINS ,TUMOR suppressor genes - Abstract
Polyphenols are capable of decreasing cancer risk. We examined the chemopreventive effects of a green tea (Camellia sinensis) extract, polyphenol extract (a mixture of blackberry (Rubus fruticosus), blackcurrants (Ribes nigrum), and added resveratrol phytoalexin), Chinese bayberry (Myrica rubra) extract, and a coffee (Coffea arabica) extract on 7,12-dimethylbenz[a]anthracene (DMBA) carcinogen-increased miR-134, miR-132, miR-124-1, miR-9-3, and mTOR gene expressions in the liver, spleen, and kidneys of CBA/Ca mice. The elevation was quenched significantly in the organs, except for miR-132 in the liver of the Chinese bayberry extract-consuming group, and miR-132 in the kidneys of the polyphenol-fed group. In the coffee extract-consuming group, only miR-9-3 and mTOR decreased significantly in the liver; also, miR-134 decreased significantly in the spleen, and, additionally, miR-124-1 decreased significantly in the kidney. Our results are supported by literature data, particularly the DMBA generated ROS-induced inflammatory and proliferative signal transducers, such as TNF, IL1, IL6, and NF-κB; as well as oncogenes, namely RAS and MYC. The examined chemopreventive agents, besides the obvious antioxidant and anti-inflammatory effects, mainly blocked the mentioned DMBA-activated factors and the mitogen-activated protein kinase (MAPK) as well, and, at the same time, induced PTEN as well as SIRT tumor suppressor genes. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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