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Simple Summary: Proholopterus chilensis (Cerambycidae: Cerambycinae) is a native species and an important damage agent in the Nothofagaceae forests of southern Chile, significantly affecting timber production in some areas, especially production of Nothofagus obliqua, with no sanitary management measures currently available. Therefore, the present research determined the predisposing factors for male–female sex approaches and identified, for the first time, some compounds that appear to mediate sexual behaviors that could be used to develop specific, safe, and effective control strategies. Interestingly, the chemical communication for mating purposes in P. chilensis preliminarily seems to include both possible "volatile or airborne" pheromones (eventually long-range ones) and short-range pheromonal compounds produced only by females, something, as far as we know, unique in Cerambycinae. During the spring–summer seasons between 2019 and 2023, in the localities of Maquehue (La Araucanía Region) and Llifén (Los Ríos Region), we collected 262 virgin Proholopterus chilensis (1 female/2.3 males) specimens emerging from the live trunks of N. obliqua trees, an atypical sex ratio in Cerambycidae, suggesting high male competition for females. Virgin specimens of both sexes were individually placed in panel traps in the field, capturing only males (n = 184) over the field study seasons and only in traps baited with females. This fact preliminarily suggests the P. chilensis females emit possible "volatile or airborne" pheromones (eventually being a long-range sex pheromone), something unusual in Cerambycinae, the subfamily to which it currently belongs, although the taxonomic status of the species is under debate. In Llifén and Santiago (Metropolitan Region), behavioral observations were conducted, which allowed us to define the conditions necessary for male–female encounters that were replicated when carrying out volatile captures (Head Space Dynamic = HSD) and collections of compounds from terminalias excised from females. In field trials, virgin females less than ten days old were more attractive than older ones and attracted males during the night, i.e., between 23:00 and 5:00 AM, when the ambient temperature exceeded 11.6 °C. The aeration of females under the conditions described above and subsequent analysis of extracts by GC-MS allowed the identification of compounds absent in males and the control, including two oxygenated sesquiterpenes, a nitrogenous compound (C20), and a long-chain hydrocarbon (C26). From the terminalia extracts, hentriacontane, heptacosane, and heneicosyl, heptacosyl and docosyl acetates were identified by GC-MS, and their roles are unknown in the development of short-distance sexual behaviors, but they could serve to mark a trail leading the male towards the female in the final stage of approach for courtship/mating. Thus, we proposed the hypothesis that P. chilensis females emit both a long-range and a trail-pheromone, which, if confirmed, would be a rare case in this family. The specific identity of the compounds obtained by HSD, as well as the activities of these chemicals and those obtained from the terminalias, should be evaluated in future behavioral studies, as well as regarding their potential to attract males under field conditions. The current document is the first report on volatiles obtained from aerations and compounds extracted from female terminalia in P. chilensis. [ABSTRACT FROM AUTHOR]

The Swarm Guiding and Communication System (SGCS) is a decision-making and information-sharing framework for robot swarms that only needs close-range peer-to-peer communication and no centralized control. Each robot makes decisions based on an incomplete virtual pheromone map that is updated on each interaction with another robot, imitating ant colonial behavior. Similar systems rely on continuous communication with no range limitations, environment modification, or centralized control. A computer simulation is developed to assess the effectiveness and robustness of the framework in covering an area. Consistency and the time needed for 99% coverage are compared with an unbiased random walk. The pheromone approach is shown to outperfom the unbiased one regardless of number of agents. Innate resilience to individual failures is also demonstrated. [ABSTRACT FROM AUTHOR]

Simple Summary: The main aim of this study was to identify antennally active pheromone components from the citrus long-horned beetle, Anoplophora chinensis, and to test them for behavioral activity. We identified three antennally active male-produced chemical components (nonanal, 4-(n-heptyloxy)butan-1-ol, and 4-(n-heptyloxy)butanal). When tested together in Y-tube behavioral assays, these three compounds were shown to be attractive to adult female A. chinensis. Pheromone production and behavior of A. chinensis are discussed in relation to other closely related Anoplophora cerambycid species. The citrus long-horned beetle, Anoplophora chinensis (Coleoptera: Cerambycidae), is a highly polyphagous species native to eastern and southeastern Asia. Detection of these beetles is dependent on visual surveys, which are inefficient and labor-intensive. The identification and development of pheromone-based lures would help improve survey efforts for A. chinensis should it become established within the US. We identified three antennally active male-produced chemical components (nonanal, 4-(n-heptyloxy)butan-1-ol, and 4-(n-heptyloxy)butanal), which were then tested for behavioral activity at our USDA Quarantine laboratory. When tested together in Y-tube behavioral assays, a three-component blend of the identified compounds was shown to be attractive to adult female A. chinensis. Pheromone production and behavior of A. chinensis and other closely related Anoplophora cerambycid species are discussed in relation to mate finding and potential monitoring. [ABSTRACT FROM AUTHOR]

Simple Summary: The number of invasive pests has increased worldwide, mainly due to international trade. This problem is further exacerbated by climate change and the continued restrictions on the quantity and number of pesticides imposed by international authorities. Delottococcus aberiae, a mealybug native to South Africa, was first detected in Europe in 2009, specifically in the Valencia Community (Spain). The identification of its sex pheromone allowed the development of a monitoring network for this pest a decade later. This network facilitated the mapping of the affected citrus area and its subsequent expansion over time. The monitoring network has revealed that from 2019 until 2023, the citrus area affected by the pest has considerably increased. This network, in conjunction with fruit damage assessments conducted at the end of the crop cycle, has also allowed us to know that the damage to the fruit is independent of the maximum annual population level of the pest. The male catches obtained during the months of March to June, when fruit setting occurs, seem to be related to economic losses due to fruit damage. The invasive mealybug Delottococcus aberiae De Lotto (Hemiptera: Pseudococcidae) has rapidly spread in the Mediterranean basin since its detection in 2009 in the Valencia Community in eastern Spain. The use of sticky traps baited with its sex pheromone, (4,5,5-trimethyl-3-methylenecyclopent-1-en-1-yl)methyl acetate, has allowed to determine the geographical distribution of D. aberiae by means of the surveillance network described in the present work. The population monitoring of the pest over a five-year period (2019–2023) has revealed an increase from 31% to 70% of the affected citrus-growing area. The monitoring network has also allowed a better understanding of the pests' biological cycle throughout the year. The populations start growing from March to June and reach their maximum in July–August. During autumn, there is a gradual decline in the population. Although the highest annual populations were detected in 2022 and 2023, the greatest crop losses were recorded in 2020 and 2021, with mean values near 18%. Data suggest that the damage responsible for fruit deformation, and thus the economic losses, are related to the population levels in spring (April–May) rather than those in summer (July–August). The findings of this study can be valuable for future research and development of effective pest control strategies. [ABSTRACT FROM AUTHOR]

Simple Summary: The rice leaf-roller Cnaphalocrocis medinalis is a migratory pest of rice. Monitoring its population is important in integrated pest management systems. Trapping them with a sex pheromone or plant odor has been used in population monitoring. We studied the physiological status of adults trapped by sex pheromones and floral odors. In the immigrant group, the number of males caught in the floral trap was greater than those caught by the sex pheromone trap. The volumes of testes in adults caught using the above two trapping methods were similar. In the local breeding group, the number of males caught by sex pheromone trapping was greater than that by floral trapping. The volume of testes was smaller in males caught in the floral odor trap compared to the pheromone trap. In the emigrant group, the adult olfactory response to the sex pheromone and floral odor was low. The number of eggs laid by the females in the local breeding group was greater in those caught in the sweep net in comparison with those caught in the floral odor trap. The rice leaf-roller Cnaphalocrocis medinalis is an important migratory pest of rice. We conducted a study to determine the physiological status of adults trapped by a sex pheromone and floral odor. In the immigrant group, the number of males trapped by the floral odor was greater than the number caught by sex pheromone trapping. The volume of testes was similar in the above two trapping methods but was smaller than in the sweep net method. The ovary developmental grade, mating rate, and number of matings of females caught in floral odor trap were higher than in those caught in the sweep net. In the local breeding group, the number of males trapped by sex pheromones was greater than the number trapped by the floral odor. The volume of testes was smaller in the floral odor trap compared to the pheromone trap group, with the largest in the sweep net group. The ovarian developmental grade, mating rate, and number of matings of females were significantly higher in the floral odor trap group than in the sweep net group. In the emigrant group, the adult olfactory response to the sex pheromone and floral odor was low. The volume of testes was larger in the sweep net group compared to the moths caught by floral odor trapping. The number of eggs laid by female immigrants trapped by the floral odor and sweep net was similar, while the number in the local breeding group was greater in moths caught with the sweep net in comparison with those caught by the floral odor trap. The difference in egg hatchability between the two trapping methods in both immigrants and local breedings was not significant. [ABSTRACT FROM AUTHOR]

The Indian meal moth, Plodia interpunctella, poses a significant threat to global agricultural products. Although pheromone-based technologies show promise for P. interpunctella management, limitations such as single-sex targeting necessitate exploring complementary strategies. Vegetable-based oils represent a potential alternative but their efficacy, sex-specificity, and interaction with sex pheromones remain unclear. To address these questions, we first examined the attraction of P. interpunctella female and male adults to 10 commercially available vegetable oils in semi-field conditions. Sesame, olive, and blended oils were the most attractive, capturing significantly more adults compared to other oils. We then evaluated the effectiveness of these three attractive oils and their combinations with P. interpunctella sex pheromones in a grain warehouse. Traps baited with these oils captured significantly more females and males compared to control traps without attractants; however, the addition of sex pheromones did not improve male capture and significantly reduced female capture, suggesting an inhibitory effect. Finally, we demonstrated that female mating status (mated vs. virgin) did not influence the attractiveness of traps baited with these effective oils. Our findings highlight the potential of standalone traps baited with blended, olive, or sesame oil as a practical, economic, and effective management strategy for P. interpunctella in storage facilities. [ABSTRACT FROM AUTHOR]

The tea tussock moth is a pest that damages tea leaves, affecting the quality and yield of tea and causing huge economic losses. The efficient asymmetric total synthesis of the sex pheromone of the tea tussock moth was achieved using commercially available starting materials with a 25% overall yield in 11 steps. Moreover, the chiral moiety was introduced by Evans' template and the key C-C bond construction was accomplished through Julia–Kocienski olefination coupling. The synthetic sex pheromone of the tea tussock moth will facilitate the subsequent assessment and implementation of pheromones as environmentally friendly tools for pest management. [ABSTRACT FROM AUTHOR]

Bee alarm pheromones are essential molecules that are present in beehives when some threats occur in the bee population. In this work, we have applied multilevel modeling techniques to understand molecular interactions between representative bee alarm pheromones and polymers such as polymethyl siloxane (PDMS), polyethylene glycol (PEG), and their blend. This study aimed to check how these interactions can be manipulated to enable efficient separation of bee alarm pheromones in portable membrane inlet mass spectrometric (MIMS) systems using new membranes. The study involved the application of powerful computational atomistic methods based on a combination of modern semiempirical (GFN2-xTB), first principles (DFT), and force-field calculations. As a fundamental work material for the separation of molecules, we considered the PDMS polymer, a well-known sorbent material known to be applicable for light polar molecules. To improve its applicability as a sorbent material for heavier polar molecules, we considered two main factors—temperature and the addition of PEG polymer. Additional insights into molecular interactions were obtained by studying intrinsic reactive properties and noncovalent interactions between bee alarm pheromones and PDMS and PEG polymer chains. [ABSTRACT FROM AUTHOR]

The paper proposes a genetic ant colony algorithm that integrates genetic and ant colony algorithms, enhancing the heuristic function of the latter, to address target point distribution issues in large well clusters. This algorithm utilizes genetic algorithms for initial pheromone distribution and employs the ant colony algorithm to achieve rapid convergence. Introducing genetic operators in each iteration addresses the ant colony system's drawbacks, including scarcity of initial pheromones, susceptibility to local optima, and slow convergence speed. The model aims to minimize the sum of horizontal displacement and intersections in line connections from wellheads to target points as its dual-objective function. It validates the effectiveness of the genetic ACO algorithm in optimizing target point allocation at wellheads through a case study, highlighting its advantages over traditional methods in reducing displacement, ensuring result stability, and preventing collisions. [ABSTRACT FROM AUTHOR]

The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a highly destructive invasive pest targeting Solanaceae crops. Its olfactory system plays a crucial role in host location, mate finding, and other behavioral activities. However, there is a notable gap in the literature regarding the characterization of its chemosensory genes. In this study, we conducted a genome-wide identification of 58 odorant receptors (ORs) of T. absoluta. The identified ORs exhibit coding sequence (CDS) lengths ranging from 1062 bp to 1419 bp, encoding proteins of 354 to 473 amino acids. Gene structure analysis showed that the majority of these ORs consist of five, seven, eight, or nine exons, collectively representing 67% of the total ORs identified. Through chromosomal mapping, we identified several tandemly duplicate genes, including TabsOR12a, TabsOR12b, TabsOR12c, TabsOR21a, TabsOR21b, TabsOR34a, TabsOR34b, TabsOR34c, TabsOR62a, and TabsOR62b. The phylogenetic analysis indicated that six TabsORs were clustered within the lepidopteran sex pheromone receptor clade, while an expansion clade containing ten TabsORs resulted from tandem duplication events. Additionally, five TabsORs were classified into a specific OR clade in T. absoluta. Furthermore, through RNA-Seq and RT-qPCR analyses, we identified five TabsORs (TabsOR21a, TabsOR26a, TabsOR34a, TabsOR34c, and TabsOR36) exhibiting female-antennae-biased expression. Our study provides a valuable foundation to further investigations into the molecular and ecological functions of TabsORs, particularly in relation to oviposition behavior. These findings provide foundational data for the future exploration of the functions of female-biased expression OR genes in T. absoluta, thereby facilitating the further development of eco-friendly attract-and-kill techniques for the prevention and control of T. absoluta. [ABSTRACT FROM AUTHOR]

The Lichen moth, Lyclene dharma dharma (Arctiidae, Lithosiinae), plays a significant role in forest ecosystem dynamics. A concise and novel method to synthesize the active sex pheromone components, (S)-14-methyloctadecan-2-one ((S)-1), (S)-6-methyloctadecan-2-one ((S)-2), and their enantiomers has been developed. Key steps in the synthesis include the use of Evans' chiral auxiliaries, Grignard cross-coupling reactions, hydroboration–oxidation, and Wacker oxidation. The synthesized sex pheromone components hold potential value for studies on communication mechanisms, species identification, and ecological management. [ABSTRACT FROM AUTHOR]

Simple Summary: The spotted lanternfly (SLF) is an invasive generalist that is spreading throughout the eastern United States. They feed in large aggregations, but the mechanisms they use to form these aggregations and find each other for mating are poorly understood. Laboratory evidence that they use pheromones is based on volatiles from their bodies and from honeydew excretions, but, due to their broad sensory capabilities, the pheromone components have been difficult to isolate and identify. This is the first evidence gathered in a field study that demonstrates, using naturally collected SLF odors as lures, that volatiles from SLF bodies and honeydew are both required to attract males at mating time and females during oviposition time. Using semiochemicals collected from spotted lanternflies Lycorma delicatula (Hemiptera: Fulgoridae) (SLF) and deployed in the field with circle traps, we demonstrated that SLF responded to SLF pheromones: in particular, this was the case for males while seeking mates and for females while ovipositing. The attractants consisted of SLF body extract emitted from diffuser lures and SLF honeydew on burlap ribbons, collected from heavily infested locations. Traps with attractants were deployed in field sites with very light SLF infestations to avoid competing signals of pre-existing aggregations. The number of SLF equivalents emitted by each diffuser per trapping period was used in a dose–response analysis. Three trees per block received either (1) a control hexane lure and a clean ribbon, (2) a lure containing SLF extract and a clean ribbon, or (3) a lure containing SLF extract and a honeydew-laden ribbon. Ten blocks were sampled three times per week for twelve weeks. We found a significant positive dose–response by males to SLF body extract only in the presence of SLF honeydew, indicating a synergistic effect between honeydew volatiles and body volatiles. This dose–response occurred for five weeks after mating started, after which males no longer responded. Subsequently, females had a significant positive dose–response to SLF extract only in the presence of honeydew when oviposition was their primary activity, continuing for two weeks, suggesting that females may use pheromones to aggregate for oviposition. The extract in the absence of honeydew did not result in a positive dose–response, nor did the hexane control. These findings suggest that SLF respond synergistically to the combination of pheromones present in both SLF honeydew and SLF bodies. Thus, combining key components from both sources may aid the development of semiochemical lures for SLF. [ABSTRACT FROM AUTHOR]

Simple Summary: This study aimed to investigate the function of the nuptial pad-secreted pheromone in anurans. By conducting transcriptome sequencing of the nuptial pads of the Yunnan pond frog, we obtained transcripts of protein pheromones that were highly expressed in the nuptial pads during the breeding season. These pheromones were homologous to the common frog amplexins and the plethodontid modulating factor (PMF) pheromone of urodeles. By means of recombinant protein expression, we obtained the single subtype of the pheromone and demonstrated its ability to shorten the duration of Yunnan pond frog pairing through behavioral experiments. The results of this study further confirm that urodeles and anurans share the PMF pheromone system. Additionally, this study validates the function of this pheromone in anurans for the first time, confirming that it serves similar functions as in urodeles, shortening the breeding process. Overall, this study provides a paradigm for exploring the function of pheromones in anurans. Chemical communication is an important mode of communication in the courtship and breeding processes of amphibians. In caudates, multiple components of sexual pheromones have been identified and functionally verified. One of these pheromone systems is plethodontid modulating factor (PMF). In anurans, the pheromone called amplexin was found in nuptial pads of ranids and was considered a member of the PMF system, yet its bio-function has not been tested. In this study, we obtained 18 amplexin transcript sequences from nuptial pads of Nidirana pleuraden (Amphibia, Ranidae) by transcriptome sequencing and found that the proteins translated by these transcripts are diversified, hydrophilic, and relatively stable. We also acquired a N. pleuraden amplexin isoform with the highest expression level in the transcriptome analysis through the prokaryotic expression system. Using two different animal behavioral experimental settings, we have tested the bio-function of the recombinant PMF protein (rPMF) in N. pleuraden's reproduction and found that the rPMF does not attract females but shortens the duration of amplexus significantly. This is the first study to verify the function of the PMF pheromone in Anura, indicating the pervasiveness of chemical communication during breeding in amphibians. [ABSTRACT FROM AUTHOR]

Simple Summary: If the existence of female sex pheromones in shrimp, Penaeus vannamei, is confirmed, they could be used to increase the larval production needed to supply shrimp farms worldwide. To gather more evidence of their existence, liposoluble compounds were extracted from the cuticle of different portions of the abdomen of adult females. These extracts were used to coat PVC tubes that were used as artificial females placed in tanks stocked with adult males. The males were monitored to determine if sexual behavior was displayed when in contact with the artificial females. The behavior observed in the shrimp males of this study provides evidence of the existence of sex pheromones located primarily in the ventral portion of the abdomen exoskeleton of shrimp females. Although the presence of female contact sex pheromones in P. vannamei has been hypothesized, to date its existence has not been proven. To gather more evidence of their existence, cuticular liposoluble extracts were obtained from the following samples of adult females to be used as the experimental treatments: (1) ventral exoskeleton of immature female (VI), (2) dorsolateral exoskeleton of immature female (DI), (3) ventral exoskeleton of mature female (VM), and (4) dorsolateral exoskeleton of mature female (DM). Polyvinyl chloride tubes (artificial females; AF) were coated with each extract and the behavior displayed by sexually mature males in contact with the AF was recorded and classified as follows: 0 = no response; 1 = contact; 2 = pushing; and 3 = prolonged contact (≥10 s). To test the hypothesis that the extracts collected from the ventral portion of the abdomen exoskeleton have a higher effect on the behavior of males than the extracts collected from the dorsolateral portion of the abdomen exoskeleton, the experiment was divided into two bioassays: Bioassay I (VI vs. DI) and Bioassay II (VM vs. DM). In each bioassay, all experimental treatments were significantly different (p > 0.05) from the CTL group (AF coated with hexane). Notably, the pushing behavior was significantly higher (p < 0.05) in the VI treatment compared to the CTL and DI treatment. These results provide evidence of the existence of contact female sex pheromones with sexual recognition function located primarily in the ventral portion of the abdomen exoskeleton of P. vannamei. [ABSTRACT FROM AUTHOR]

Pollination by sexual deception specifically attracts male insects, through the floral scent and particular morphological features of the flower that serve as visual and tactile stimuli. The unique bond between the Ophrys speculum orchid and the male Dasyscolia ciliata wasp primarily stems from a few distinctive semiochemicals that mimic the female wasp's sex pheromone, although the floral scent comprises a variety of compounds. An osmophore producing highly volatile compounds has been documented in four close relatives of O. speculum and is now being also investigated in this species. Given the existing debates regarding the structure of the labellum and stigmatic cavity in O. speculum, this study details their micromorphology. Additionally, comparisons of O. speculum flowers and female D. ciliata wasps under stereomicroscopy and scanning electron microscopy are conducted to seek new evidence of visual and tactile mimicry. The findings confirm that (i) an osmophore is present at the apical margin of the labellum in O. speculum flowers; (ii) the labellum features a distinct basal field homologous to those found in other Ophrys species; and (iii) the basal labellum region closely mimics the female wasp's thorax and wings. The implications of these novel floral features are discussed within an evolutionary context. [ABSTRACT FROM AUTHOR]

A concise synthesis of the sex pheromones of elm spanworm as well as painted apple moth has been achieved. The key steps were the alkylation of acetylide ion, Sharpless asymmetric epoxidation and Brown's P2-Ni reduction. This approach provided the sex pheromone of the elm spanworm (1) in 31% total yield and those of the painted apple moth (2, 3) in 26% and 32% total yields. The ee values of three final products were up to 99%. The synthesized pheromones hold promising potential for use in the management and control of these pests. [ABSTRACT FROM AUTHOR]

The paulownia bagworm, Clania variegata Snell, is an economically important pest of agriculture and forests. The sex pheromone of this pest and its stereoisomers were synthesized, and two of the stereoisomers were prepared for the first time. Our strategy was efficient and mainly included the ring-opening reaction of (S)-2-methyloxirane, the coupling of chiral sulfonate, the oxidative cleavage of olefin, and Yamaguchi esterification. Moreover, the overall yields of our synthesis were 23–29%, with eight steps in the longest route. [ABSTRACT FROM AUTHOR]

In ascomycetous fungi, sexual mate recognition requires interaction of the Ste2 receptor protein produced by one partner with the α-factor peptide pheromone produced by the other partner. In some fungi, Ste2 is further needed for chemotropism towards plant roots to allow for subsequent infection and colonization. Here, we investigated whether this is also true for the pine pitch canker fungus, Fusarium circinatum, which is a devastating pathogen of pine globally. Ste2 knockout mutants were generated for two opposite mating-type isolates, after which all strains were subjected to chemotropism assays involving exudates from pine seedling roots and synthetic α-factor pheromone, as well as a range of other compounds for comparison. Our data show that Ste2 is not required for chemotropism towards any of these other compounds, but, in both wild-type strains, Ste2 deletion resulted in the loss of chemotropism towards pine root exudate. Also, irrespective of mating type, both wild-type strains displayed positive chemotropism towards α-factor pheromone, which was substantially reduced in the deletion mutants and not the complementation mutants. Taken together, these findings suggest that Ste2 likely has a key role during the infection of pine roots in production nurseries. Our study also provides a strong foundation for exploring the role of self-produced and mate-produced α-factor pheromone in the growth and overall biology of the pitch canker pathogen. [ABSTRACT FROM AUTHOR]

Simple Summary: Insects are covered with chemicals called hydrocarbons that are involved in chemical communication (mainly in mating) as well as in preventing the insect from drying out and protecting it from harmful environmental factors. Although we know a great deal about these molecules in laboratory strains, little is known about their variation and evolution in nature. We studied Drosophila melanogaster flies from Dijon, France, which we captured using traps baited with various fruits. We set up lines from these flies and studied their hydrocarbons on capture and after up to 40 generations in the laboratory. We initially observed a lot of natural variability, but this soon became more limited as the strains adapted to life in the laboratory. In some of the lines, females flies had a hydrocarbon profile previously seen only in tropical populations: this may indicate that this globally distributed species is adapting to increased temperatures caused by climate change. These hydrocarbons can be used as sensitive markers of climate change, as long as they are studied before insects become adapted to laboratory conditions. Experimental studies of insects are often based on strains raised for many generations in constant laboratory conditions. However, laboratory acclimation could reduce species diversity reflecting adaptation to varied natural niches. Hydrocarbons covering the insect cuticle (cuticular hydrocarbons; CHCs) are reliable adaptation markers. They are involved in dehydration reduction and protection against harmful factors. CHCs can also be involved in chemical communication principally related to reproduction. However, the diversity of CHC profiles in nature and their evolution in the laboratory have rarely been investigated. Here, we sampled CHC natural diversity in Drosophila melanogaster flies from a particular location in a temperate region. We also measured cis-Vaccenyl acetate, a male-specific volatile pheromone. After trapping flies using varied fruit baits, we set up 21 D. melanogaster lines and analysed their pheromones at capture and after 1 to 40 generations in the laboratory. Under laboratory conditions, the broad initial pheromonal diversity found in male and female flies rapidly changed and became more limited. In some females, we detected CHCs only reported in tropical populations: the presence of flies with a novel CHC profile may reflect the rapid adaptation of this cosmopolitan species to global warming in a temperate area. [ABSTRACT FROM AUTHOR]

Simple Summary: The tea geometrid (Ectropis obliqua (Prout)) is a major defoliator of tea plants and seriously affects the tea quality and yield in China. The females release three Type-II sex pheromone components for mate communication, but knowledge about the biosynthesis of these compounds is still limited. In this study, we screened for the candidate genes in the sex pheromone biosynthetic pathway with a combination of comparative RNAseq, phylogenetic relationship, and tissue expression pattern analyses. A total of seven tentative biosynthetic enzymes were identified, including two ELOs (ELO3 and ELO5), two FARs (FAR2 and FAR9), one DEC (CYP4G173), one LIP (LIP1), and one epoxidase (CYP340BD1). Overall, our results provide a foundation for further functional elucidation of the vital genes involved in E. obliqua sex pheromone biosynthesis. Ectropis obliqua, a notorious tea pest, produces a Type-II sex pheromone blend for mate communication. This blend contains (Z,Z,Z)-3,6,9-octadecatriene, (Z,Z)-3,9-cis-6,7-epoxy-octadecadiene, and (Z,Z)-3,9-cis-6,7-epoxy-nonadecadiene. To elucidate the genes related to the biosynthesis of these sex pheromone components, transcriptome sequencing of the female E. obliqua pheromone gland and the abdomen without pheromone gland was performed. Comparative RNAseq analyses identified 52 putative genes, including 7 fatty acyl-CoA elongases (ELOs), 9 fatty acyl-CoA reductases (FARs), 1 decarbonylase (DEC), 3 lipophorins (LIPs), and 32 cytochrome P450 enzymes (CYPs). Tissue expression profiles revealed that two ELOs (ELO3 and ELO5), two FARs (FAR2 and FAR9), one DEC (CYP4G173), and one LIP (LIP1) displayed either abdomen-centric or -specific expression, suggesting potential roles in sex pheromone biosynthesis within the oenocytes of E. obliqua. Furthermore, the tissue expression patterns, combined with phylogenetic analysis, showed that CYP340BD1, which was expressed specifically and predominantly only in the pheromone gland, was clustered with the previously reported epoxidases, highlighting its potential role in the epoxidation of the unsaturated polytriene sex pheromone components. Collectively, our research provides valuable insights into the genes linked to sex pheromone biosynthesis. [ABSTRACT FROM AUTHOR]

The impact of organometallic chemistry on the terpene field only really blossomed in the 1960s and 1970s with the realisation that carbon–carbon bond formation under mild conditions could be achieved by using nickel or iron carbonyls as synthetic reagents. Concomitantly, the development of palladium derivatives capable of the controlled coupling of isoprene units attracted the attention of numerous highly talented researchers, including future Nobel laureates. We discuss briefly how early work on the syntheses of simple monoterpenes soon progressed to sesquiterpenes and diterpenes of increasing complexity, such as humulene, flexibilene, vitamin A, or pheromones of commercial value, in particular those used in perfumery (muscone, lavandulol), or grandisol and red scale pheromone as replacements for harmful pesticides. As the field progressed, there has been more emphasis on developing organometallic routes to enantiopure rather than racemic products, as well as gaining precise mechanistic data on the transformations, notably the course of metal-promoted molecular rearrangements that have long been a feature of terpene chemistry. We note the impact of the enormously enhanced analytical techniques, high-field NMR spectroscopy and X-ray crystallography, and their use to re-examine the originally proposed structures of terpenes and their organometallic derivatives. Finally, we highlight the very recent ground-breaking use of the crystalline sponge method to acquire structural data on low-melting or volatile terpenes. The literature cited herein covers the period 1959 to 2023. [ABSTRACT FROM AUTHOR]

Pheromone-binding proteins (PBPs) are specific odorant-binding proteins that can specifically recognize insect pheromones. Through transcriptional analysis of the antennae of adult Endoclita signifer, EsigPBP3 was discovered and identified, and EsigPBP3 was found to be highly expressed in the antennae of male moths. Based on the binding characteristics and ability of EsigPBP3, we can find the key ligands and binding site to consider as a target to control the key wood bore E. signifier. In this study, the fluorescence competitive binding assays (FCBA) showed that EsigPBP3 had a high binding affinity for seven key eucalyptus volatiles. Molecular docking analysis revealed that EsigPBP3 had the strongest binding affinity for the sexual pheromone component, (3E,7E)-4,7,11-trimethyl-1,3,7,10-dodecatetraene. Furthermore, same as the result of FCBA, the EsigPBP3 exhibited high binding affinities to key eucalyptus volatiles, eucalyptol, α-terpinene, (E)-beta-ocimene, (−)-β-pinene, and (−)-α-pinene, and PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 are key sites. In summary, EsigPBP3 exhibits high binding affinity to male pheromones and key volatile compounds and the crucial binding sites PHE35, MET7, VAL10, PHE38, ILE52, and PHE118 can act as targets in the recognition of E. signifier pheromones. [ABSTRACT FROM AUTHOR]

Plant hormones and pheromones are natural compounds involved in the growth, development, and reproductive processes. There is a plethora of studies on hormones and pheromones in terrestrial plants, but such investigations are few in the phycological literature. There are striking similarities between the chemical diversity, biosynthetic processes, roles, and actions of hormones and pheromones in both higher angiospermic plants and algae. However, there are substantial knowledge gaps in understanding the genes responsible for hormone biosynthesis and regulation in algae. Efforts have focused on identifying the genes and proteins involved in these processes, shedding light on lateral gene transfer and evolutionary outcomes. This comprehensive review contributes to benchmarking data and essential biotechnological tools, particularly for the aquaculture industry where seaweed is economically crucial. Advanced techniques in plant hormones and pheromones can revolutionize commercial aquaculture by using synthetic analogs to enhance growth, yield, and reproductive control, thereby addressing seasonal limitations and enabling sustainable seedling production. To the best of our knowledge, this is the first comprehensive review that focuses on biosynthetic pathways and modes of action (of five plant hormones and five pheromones), roles (of 11 hormones and 29 pheromones), and extraction protocols (of four hormones and six pheromones) reported in the phycological domain. [ABSTRACT FROM AUTHOR]

Simple Summary: Sex pheromone plays an important role in mediating mate communication for the tea black tussock moth, Dasychira baibarana, which is a devastating pest in Chinese tea plantations. The sex pheromone of this pest is a ternary blend, including two typical Type-II epoxides and one unsaturated ketone. This study investigated P450 candidates that are associated with the biosynthesis of the sex pheromone components. With a combination of comparative RNAseq, phylogenetic, and tissue expression pattern analysis, one CYP4G with abdomen specifically predominant expression profile was likely to be the P450 decarbonylase, and the pheromone-gland specifically and most abundant CYP341B65 was the most promising epoxidase. This research laid a valuable foundation not only for further functional characterization of P450 decarbonylase and epoxidase in the sex pheromone biosynthetic pathway but also for understanding the physiological functions and functional diversity of the CYP gene superfamily in the D. baibarana. The tea black tussock moth (Dasychira baibarana), a devastating pest in Chinese tea plantations, uses a ternary Type-II pheromone blend containing (3Z,6Z)-cis-9,10-epoxyhenicosa-3,6-diene (Z3,Z6,epo9-21:H), (3Z,6Z,11E)-cis-9,10-epoxyhenicosa-3,6,11-triene (Z3,Z6,epo9,E11-21:H), and (3Z,6Z)-henicosa-3,6-dien-11-one (Z3,Z6-21:11-one) for mate communication. To elucidate the P450 candidates associated with the biosynthesis of these sex pheromone components, we sequenced the female D. baibarana pheromone gland and the abdomen excluding the pheromone gland. A total of 75 DbP450s were identified. Function annotation suggested six CYPs were orthologous genes that are linked to molting hormone metabolism, and eight antennae specifically and significantly up-regulated CYPs may play roles in odorant processing. Based on a combination of comparative RNAseq, phylogenetic, and tissue expression pattern analysis, one CYP4G with abdomen specifically predominant expression pattern was likely to be the P450 decarbonylase, while the pheromone-gland specifically and most abundant CYP341B65 was the most promising epoxidase candidate for the D. baibarana sex pheromone biosynthesis. Collectively, our research laid a valuable basis not only for further functional elucidation of the candidate P450 decarbonylase and epoxidase for the sex pheromone biosynthesis but also for understanding the physiological functions and functional diversity of the CYP gene superfamily in the D. baibarana. [ABSTRACT FROM AUTHOR]

Simple Summary: Pheromones, such as sex pheromones, aggregation pheromones, and alarm pheromones, are the basis for insect aggregation, mating, and other behaviors. Compared with the structures of sex pheromones, those of aggregation pheromones are more complex, especially in cucujoid grain beetles, which produce macrocyclic lactones as aggregation pheromones. So far, there is limited research studying the biosynthesis of macrocyclic lactones at the molecular level. Two aggregation pheromone components of the rusty grain beetle, Cryptolestes ferrugineus, have been identified to be biosynthesized via the mevalonate (MVA) pathway and fatty acid synthesis (FAS) pathway, with their productions affected by starvation. Therefore, we conducted a comparative transcriptomics analysis of hypothetical pheromone production sites in C. ferrugineus under starvation stress. Additionally, significantly enriched metabolic pathways and down-regulated genes directly involved in the biosynthesis and regulation of aggregation pheromones via the MVA and FAS pathways, respectively, were identified, as well as some juvenile hormone (JH)- and insulin-related genes, which are of great significance for elucidating the synthesis and regulatory mechanisms of cucujoid grain beetle pheromones. Pheromones are the basis of insect aggregation, mating, and other behaviors. Cucujoid grain beetles produce macrocyclic lactones as aggregation pheromones, yet research on their biosynthesis at the molecular level remains limited. The rusty grain beetle, C. ferrugineus, is an important economic species in China. Although two aggregation pheromone components have been identified, their suspected biosynthesis via the MVA pathway and the FAS pathway lacks molecular elucidation. Previous evidence supports that starvation affects the production of aggregation pheromones. Therefore, we constructed comparative transcriptome libraries of pheromone production sites in C. ferrugineus under starvation stress and identified genes related to pheromone biosynthesis and hormone regulation. A total of 2665 genes were significantly differentially expressed, of which 2029 genes were down-regulated in starved beetles. Putative C. ferrugineus genes directly involved in pheromone biosynthesis were identified, as well as some genes related to the juvenile hormone (JH) pathway and the insulin pathway, both of which were depressed in the starved beetles, suggesting possible functions in pheromone biosynthesis and regulation. The identification of genes involved in macrolide lactone biosynthesis in vivo holds great significance, aiding in the elucidation of the synthesis and regulatory mechanisms of cucujoid grain beetle pheromones. [ABSTRACT FROM AUTHOR]

Biological control, a well-established plant protection method, has garnered substantial attention in recent decades. Various approaches, including biological control agents (BCA), catch crops, biofumigation, sticky traps, and pheromones, have been extensively explored. While the effectiveness of these methods varies depending on specific circumstances, their collective significance has grown amid mounting pressures to curtail or eliminate conventional synthetic plant protection products. Previous review articles have highlighted the benefits of using two or more BCAs simultaneously, yet limited information exists regarding the concurrent use of diverse biological control methods. This comprehensive review incorporates a thorough literature search to assess the benefit of concurrently employing two or more of these methods, followed by a discussion on perspectives of holistic management and mimicking complex natural systems, shedding light on the vast potential and need for further research in this domain. [ABSTRACT FROM AUTHOR]

Simple Summary: All commercial swine farms require replacement gilts. Gilts are grown in pens and are commonly exposed to boars to induce estrus. Boar exposure to peri-puberal gilts is time-consuming, with variable results on commercial farms. Our goal was to determine whether a novel boar pheromone (Boar Better®; BB) could replace a live boar, accelerate the onset of puberty, and obtain equal or better percentages of gilts bred. A novel sprayer that allowed gilts to self-administer BB (a putative boar priming pheromone for gilts) at will. The sprayer is an environmental enrichment (EE) device that pigs root, push, and chew. The EE device can all gilts to self-deliver BB during the late pre-puberal period, which may save labor and improve worker and gilt safety by reducing the use of a live boar. In this work, gilts were exposed to either boars on a daily basis or BB self-administered in the EE sprayer. In conclusion, BB induced the same or more gilts to be identified in estrus compared to live boar exposure, and injured fewer gilts while reducing the labor and boar maintenance costs. The data support the idea that BB acted as a priming pheromone to stimulate the onset of estrus in gilts without a boar. Labor is in short supply in animal agriculture. One time-consuming task is estrus detection in gilts. Stimulation with a live boar causes the onset of puberty in young gilts. Typically, a live boar is used to stimulate and identify estrus in the gilts by exposing the gilts to him. Recently, a boar pheromone (BB) was developed to replace the use of a live boar for sows. Additionally, a novel automatic sprayer used as environmental enrichment (EE) by gilts for the self-administration of BB has been developed by this laboratory. A commercial study was conducted to determine whether the use of a live boar could be replaced with a simple EE sprayer, allowing gilts to self-administer BB. Our objective was to determine whether the number and percentage of gilts in estrus obtained using live boars was comparable to self-administration using an EE sprayer containing BB. A total of 242 gilts were randomly assigned to either a live boar (BOAR) or BB self-administration using the environmental enrichment (EE) sprayer. Gilts began simultaneous exposure to either the BOAR or the BB when they were about 4–5 months of age and this continued until they were found in estrus or were injured, died, or never cycled about 2 months later. A total of 83.3% of gilts with exposure to BOAR were identified in estrus and bred, while exposure to BB resulted in 92.9% of gilts reaching puberty and being bred (p < 0.05). The days to reach estrus were 11 days longer for gilts exposed to BB than BOAR. Eight percent more gilts were injured by the BOAR than by using BB (and no boar). The use of BB as a priming pheromone could prevent gilt injuries, save labor, and reduce costs for pig farmers while not inhibiting reproductive output. [ABSTRACT FROM AUTHOR]

Simple Summary: Weaning is one of the most important stress events in the life of pigs, increasing the risk for health problems and reduced performance. This study investigated the effect of pheromones on the performance of nursery pigs. In total, 24 batches of weaned piglets from a commercial farm (216 piglets per batch) were included. Half of the batches were treated, while the other half served as controls. Piglets of treated groups were exposed to a synthetic analog of the maternal pig appeasing pheromone (PAP) (SecurePig®, Signs, Avignon, France). The product consisted of a gel block containing the product and the pheromones diffused slowly in the room. The treatment with pheromones did not significantly influence the performance of the piglets (p > 0.05). The median values of average daily gain (318 vs. 305 g/day), feed conversion ratio (1.64 vs. 1.70), and number of antimicrobial treatment days (16.9 vs. 17.3 days) were numerically better in the nursery pigs exposed to the pheromones compared with the control groups. Mortality however was numerically higher in the treated groups (4.4 vs. 3.2%). In this farm, pigs exposed to the pheromone treatment during the nursery did not show a significant performance increase. Weaning is one of the most important stress events in the life of pigs, increasing the risk for health problems and reduced performance. The release of pheromones in pig stables can be considered an environmental enrichment and alleviate the negative effects of weaning stress in nursery pigs. The present study investigated the effect of synthetic pheromones on the performance of nursery pigs. The effect of positive handling of sows in the farrowing house on the performance of the offspring in the nursery was also investigated. The study was performed in a commercial pig farm and included 24 batches of weaned piglets (216 piglets per batch). Half of the batches originated from sows exposed to positive handling. This implied that music was played from 6.00 a.m. to 6.00 p.m. from the moment the sows entered the farrowing house until weaning and that they were subjected to backscratching from the day they entered the farrowing unit the day of farrowing. During the nursery period, half of the batches were treated, and half served as controls. Piglets of treated groups were exposed to a synthetic analog of the maternal pig appeasing pheromone (PAP) (SecurePig®, Signs, Avignon, France). The product consisted of a gel block from which the pheromones were slowly released into the room. Different performance parameters were measured during the nursery period. Neither the sow treatment nor the treatment with pheromones significantly influenced the performance of the piglets during the nursery period (p > 0.05). The median values (95% confidence interval) of average daily gain, namely 318 (282–338) vs. 305 (272–322) g/day, feed conversion ratio, namely 1.64 (1.51–1.71) vs. 1.70 (1.57–1.75), and number of antimicrobial treatment days, namely 16.9 (9.6–25.0) vs. 17.3 (9.5–25.0) days, were numerically better in the nursery pigs exposed to the pheromones compared to the control groups. Mortality however was numerically higher in the treated groups, namely 4.4 (2.8–6.8) vs. 3.2 (0.9–4.2)%. Under the conditions of the present production system, pigs exposed to the pheromone treatment during the nursery did not show a significant performance increase. [ABSTRACT FROM AUTHOR]

Pheromone-binding proteins (PBPs) play important roles in binding and transporting sex pheromones. However, the PBP genes identified in coleopteran insects and their information sensing mechanism are largely unknown. Cyrtotrachelus buqueti (Coleoptera: Curculionidae) is a major insect pest of bamboo plantations. In this study, a novel PBP gene, CbuqPBP2, from C. buqueti was functionally characterized. CbuqPBP2 was more abundantly expressed in the antennae of both sexes than other body parts, and its expression level was significantly male-biased. Fluorescence competitive binding assays showed that CbuqPBP2 exhibited the strongest binding affinity to dibutyl phthalate (Ki = 6.32 μM), followed by styrene (Ki = 11.37 μM), among twelve C. buqueti volatiles. CbuqPBP2, on the other hand, showed high binding affinity to linalool (Ki = 10.55), the main volatile of host plant Neosinocalamus affinis. Furthermore, molecular docking also demonstrated the strong binding ability of CbuqPBP2 to dibutyl phthalate, styrene, and linalool, with binding energy values of −5.7, −6.6, and −6.0 kcal/mol, respectively, and hydrophobic interactions were the prevailing forces. The knockdown of CbuqPBP2 expression via RNA interference significantly reduced the electroantennography (EAG) responses of male adults to dibutyl phthalate and styrene. In conclusion, these results will be conducive to understanding the olfactory mechanisms of C. buqueti and promoting the development of novel strategies for controlling this insect pest. [ABSTRACT FROM AUTHOR]

Simple Summary: Rosalia batesi Harold (Cerambycidae) is a longhorned beetle endemic to Japan distributed on the mainland from Hokkaido (north) to Kyushu (south). The beautiful appearance of the adults is renowned not only in Japan but worldwide. The larvae feed in the trunks of broad-leaf trees. Females were attracted to males in laboratory bioassays, which suggested that males produce a sex pheromone. We collected volatiles from R. batesi females and males and found a single male-specific compound. It was identified as 3,5-dimethyl-6-(1-methylbutyl)-pyran-2-one, which is the same compound identified as the pheromone of its European congener R. alpina. This alkylated pyrone is a unique structure among known cerambycid pheromones. In field bioassays carried out with the racemic synthetic pheromone in Japan, both sexes of R. batesi were attracted in significant numbers in a ratio of almost 1:1, indicating that the compound is an aggregation-sex pheromone. The longhorned beetle Rosalia batesi Harold (Coleoptera; Cerambycidae) is endemic to Japan, where its range extends from Hokkaido to Kyushu. The colorful adults are well-known to entomologists and collectors worldwide. It is a hardwood-boring species with larvae that develop in dead broad-leaf trees. In laboratory bioassays, females were attracted to males, which suggested that males produce a sex pheromone. The congeneric species R. alpina is native to Europe, and another congener, R. funebris, is distributed in North America. The pheromone components produced by males of these species had been previously identified as two compounds from different biosynthetic pathways. In the present study, volatiles were collected from beetles of both sexes, and the analyses of the resulting extracts revealed a single male-specific compound, which was identified as 3,5-dimethyl-6-(1-methylbutyl)-pyran-2-one; this is the same compound as the pheromone of the European R. alpina. This alkylated pyrone structure is, so far, unique among known cerambycid pheromones. In field bioassays with traps baited with the racemic synthetic pheromone, significant numbers of both sexes of R. batesi were attracted in an approximately equal ratio, indicating that the compound is an aggregation-sex pheromone rather than a sex pheromone. [ABSTRACT FROM AUTHOR]

Pathogens can influence the physiology and behavior of both animal and plant hosts in a manner that promotes their own transmission and dispersal. Recent research focusing on insects has revealed that these manipulations can extend to the production of pheromones, which are pivotal in chemical communication. This review provides an overview of the current state of research and available data concerning the impacts of bacterial, viral, fungal, and eukaryotic pathogens on chemical communication across different insect orders. While our understanding of the influence of pathogenic bacteria on host chemical profiles is still limited, viral infections have been shown to induce behavioral changes in the host, such as altered pheromone production, olfaction, and locomotion. Entomopathogenic fungi affect host chemical communication by manipulating cuticular hydrocarbons and pheromone production, while various eukaryotic parasites have been observed to influence insect behavior by affecting the production of pheromones and other chemical cues. The effects induced by these infections are explored in the context of the evolutionary advantages they confer to the pathogen. The molecular mechanisms governing the observed pathogen-mediated behavioral changes, as well as the dynamic and mutually influential relationships between the pathogen and its host, are still poorly understood. A deeper comprehension of these mechanisms will prove invaluable in identifying novel targets in the perspective of practical applications aimed at controlling detrimental insect species. [ABSTRACT FROM AUTHOR]

Pheromones play a vital role in regulating fish behavior, including reproduction, aggregation, hazard recognition and food location. To gain a better understanding of chemical communication in fish produced by density changes, this study analyzed the metabolites released by turbot (Scophthalmus maximus) under different stocking densities. The experiment was conducted at low (LD: 3.01 kg/m2), medium (MD: 6.62 kg/m2) and high (HD: 10.84 kg/m2) densities for 15 days. High-throughput non-targeted metabolomics (LC-MS/MS) was used to identify variations in metabolites released into the aquatic environment by turbot at different densities. Results showed that 29 and 47 metabolites were significantly upregulated in the MD and HD groups, respectively, compared with the LD group. Among them, hexadecanedioic acid, xanthine, phenethylamine, proline and styrene were significantly upregulated in the MD vs. LD, HD vs. MD and HD vs. LD. The VIP diagram of OPLS-DA alignment showed that phenethylamine was the most important metabolite shared by MD vs. LD, HD vs. MD and HD vs. LD. Key gene changes in the GH/IGF-1 signaling pathway, HPI axis of turbot were studied using qRT-PCR for density treatment. The results demonstrated that the expression of GH, GHR and IGF-1 was significantly lower, while the expression of CRH and ACTH was higher in the HD group. Additionally, plasma levels of cortisol, glucose, triglycerides and T3 were also highest in the HD group compared with the LD and MD groups. Phenylethylamine concentration was positively correlated with the HPI axis and negatively correlated with the GH/IGF-1 signaling pathway. To investigate the impact of phenethylamine accumulation on turbot, an acute treatment experiment with phenethylamine was set up. Its concentration in the aquatic environment was set at 0 (CON), 10−7 (LP) and 10−5 (HP) mol/L via exogenous addition, and turbot were exposed to these environments for 2 days. There was a high degree of concordance between the GH/IGF-1 signaling pathway (GH, GHR, IGF-1), HPI axis (CRH, ACTH) and plasma physiological changes (cortisol, glucose, triglycerides, T3) in the phenethylamine-treated group and the density-treated group. Therefore, accumulation of phenethylamine with increasing stocking density may be a potential cause of density stress. Phenylethylamine has a dose-dependent and trace effect as a pheromone. [ABSTRACT FROM AUTHOR]

Simple Summary: Pet air travel has increased in the last decade, and 6% of pets in the US board a plane every year. Dogs have been reported to make up 58% of pets travelling worldwide. Despite this, there is little data available about air travel in dogs. A total of 635 questionnaires were collected from dog owners whose dogs had travelled by air in the last 12 months to obtain initial data on international dog air travel and how well dogs cope with air travel physically, mentally, and emotionally. Data on how dogs were being prepared for air travel, specifically regarding stress management, were also collected. Results showed that most dogs cope with and recover well from air travel but that there is a group of individuals who suffer physical, mental, and emotional ill health consequences during or after air travel, including death. Most dog owners planned air travel themselves, and over half did not seek professional advice. Stress management products such as anxiolytic medication and supplements or pheromones were only used in one-quarter of canine travellers. This study presents opportunities for all stakeholders of pet air travel, including owners, veterinarians, airlines, airports, and pet shippers, to improve pet welfare during air travel. It is estimated that 2 million domestic animals travel on commercial flights every year in the US alone and that dogs make up 58% of pets travelling worldwide. There has been little research on the welfare effects of air travel on dogs. The purpose of this owner-reported study was to understand how well dogs cope with and recover from air travel from a physical, mental, and emotional health perspective. An online survey questionnaire was distributed globally to pet owners whose dogs had travelled by air in the last 12 months, and the results were collected and analysed. Information was received about dog and owner demographics, logistics, and preparation for travel, as well as the dog's experience of air travel. Results showed that most dogs cope with and recover well from air travel but that there is a group of individuals who suffer physical, mental, and emotional ill health consequences during or after air travel, including death. Stress management products such as anxiolytic medication, supplements, and pheromones were underutilised and, in some instances, actively discouraged. More education of all stakeholders of pet air travel is needed to improve the physical, mental, and emotional health and welfare of canine air travellers. [ABSTRACT FROM AUTHOR]

Simple Summary: The moth Loxostege sticticalis (Lepidoptera: Pyralidae) is a typical migrant insect pest found in North America, Eastern Europe, and Asia. The natural pheromone compounds of adult moths are involved in courtship and mating behavior. In this article, we examine the peripheral mechanisms of sex communication, demonstrating that the pheromone receptor (PR) LstiPR2 responds specifically to the major sex pheromone compound E11-14:OAc, which results in the activation of the "a" neuron in sensilla trichodea. The other four LstiPRs showed no response to any sex pheromone compounds. This study will contribute to a new, environmentally friendly strategy for integrated pest management of L. sticticalis. Lepidopteran insects mainly rely on sex pheromones to complete sexual communications. Pheromone receptors (PRs) are expressed on the olfactory receptor neurons (ORNs) of the sensilla trichodea and play an essential role in sexual communication. Despite extensive investigations into the mechanisms of peripheral recognition of sex pheromones in Lepidoptera, knowledge about these mechanisms in L. sticticalis remains limited. In this study, five candidate LstiPRs were analyzed in a phylogenetic tree with those of other Lepidopteran insects. Electroantennography (EAG) assays showed that the major sex pheromone component E11-14:OAc elicited a stronger antennal response than other compounds in male moths. Moreover, two types of neurons in sensilla trichodea were classified by single sensillum recordings, of which the "a" neuron specifically responded to E11-14:OAc. Five candidate PRs were functionally assayed by the heterologous expression system of Xenopus oocytes, and LstiPR2 responded to the major sex pheromone E11-14:OAc. Our findings suggest that LstiPR2 is a PR sensitive to L. sticticalis's major sex pheromone compound, E11-14:OAc. Furthermore, this study offers valuable insights into the sexual communication behavior of L. sticticalis, forming a foundation for further analysis of the species' central nervous system. [ABSTRACT FROM AUTHOR]

Agricultural crop productivity relies on the application of chemical pesticides to reduce pest and pathogen damage. However, chemical pesticides also pose a range of ecological, environmental and economic penalties. This includes the development of pesticide resistance by insect pests and pathogens, rendering pesticides less effective. Alternative sustainable crop protection tools should therefore be considered. Semiochemicals are signalling molecules produced by organisms, including plants, microbes, and animals, which cause behavioural or developmental changes in receiving organisms. Manipulating semiochemicals could provide a more sustainable approach to the management of insect pests and pathogens across crops. Here, we review the role of semiochemicals in the interaction between plants, insects and microbes, including examples of how they have been applied to agricultural systems. We highlight future research priorities to be considered for semiochemicals to be credible alternatives to the application of chemical pesticides. [ABSTRACT FROM AUTHOR]

Simple Summary: Understanding insect olfactory perception can lead to more effective and specific ways to manage pests. In fact, odors have two components: (1) the quality, defined as its chemical composition, and (2) the quantity, defined by the concentration of the odor. The quantitative aspect is often missing and we aim to provide valuable information on the quantitative aspect of the odors that attract the western flower thrips, an insect that damages crops. In this study, we tested how the western flower thrips responded to different odors in an olfactometer. We measured the odor concentrations carried by the air and found that the thrips were most attracted to a pheromone at very low concentrations, much lower than the other odors we tested. Our results show that the attraction of western flower thrips to an odor depends on its quantitative aspect and could help to develop better ways of managing this pest. An understanding of insect olfaction allows for more specific alternative methods of pest control. We evaluated the responses of the western flower thrips (WFT, Frankliniella occidentalis) in a Y-olfactometer to estimate gas-phase concentrations of the aggregation pheromone neryl (S)-2-methylbutanoate and known kairomones such as methyl isonicotinate, (S)-(-)-verbenone, and p-anisaldehyde. The gas-phase concentrations of these compounds were obtained from the release rates measured in dynamic headspace cells. The compounds were collected from the headspace using dried solid-phase extraction (SPE) cartridges and analyzed with a triple quadrupole GC-MS/MS. We observed that the aggregation pheromone significantly attracted WFT females at doses of 10 and 100 µg, whereas methyl isonicotinate and p-anisaldehyde significantly attracted WFT females at the highest dose. Verbenone did not produce any significant results. A completely different picture was obtained when the gas-phase concentrations were considered. The minimal gas-phase concentrations of the pheromone required to attract WFT females was 0.027 ng/mL, at least 100 times lower than that of the other two compounds. The relevance and implications of our results are discussed in light of the insect's biology and pest management methods. [ABSTRACT FROM AUTHOR]

Simple Summary: Evidence suggesting that the invasive species spotted lanternfly (SLF), Lycorma delicatula, may use pheromones has previously been shown. We hypothesized that sunlight might play a key role in SLF pheromone production based on observations that SLF adults spend time in the upper canopies of their host trees and their affinity for ultraviolet light. In this study, extracts from SLF nymphs and adults were either exposed to simulated sunlight (photo-degradation), or not exposed to light (crude), while volatiles were collected. Attraction of SLF nymphs and adults to volatiles from photo-degraded and crude samples, and their residues, was measured in dual-choice bioassays. SLF nymphs and adults responded differently to either residues or volatiles of both crude and photo-degraded body extracts. Photo-degradation did not increase the attraction of SLF individuals tested in two-choice bioassays, except for third instars. However, SLF males chose the residue from photo-degraded extracts over the residue from crude extracts of females. The results suggest that photo-degradation may play a role in sex-specific short-range communication, but photo-degradation did not appear to play a substantial role in long-range intraspecific chemical communication in SLF. This study produced additional evidence that SLF likely use pheromones in aggregation and mating. Since its discovery in North America in 2014, the spotted lanternfly (SLF), Lycorma delicatula, has become an economic, ecological, and nuisance pest there. Developing early detection and monitoring tools is critical to their mitigation and control. Previous research found evidence that SLF may use pheromones to help locate each other for aggregation or mating. Pheromone production necessitates specific conditions by the insects, and these must be investigated and described. A chemical process called photo-degradation has been described as a final step in the production of pheromones in several diurnal insect species, in which cuticular hydrocarbons were broken down by sunlight into volatile pheromone components. In this study, photo-degradation was investigated as a possible pheromone production pathway for SLF. Extracts from SLF mixed-sex third and fourth nymphs and male or female adults were either exposed to simulated sunlight to produce a photo-degradative reaction (photo-degraded), or not exposed to light (crude), while volatiles were collected. Behavioral bioassays tested for attraction to volatiles from photo-degraded and crude samples and their residues. In third instars, only the volatile samples from photo-degraded mixed-sex extracts were attractive. Fourth instar males were attracted to both crude and photo-degraded residues, and volatiles of photo-degraded mixed-sex extracts. Fourth instar females were attracted to volatiles of crude and photo-degraded mixed-sex extracts, but not to residues. In adults, only males were attracted to body volatiles from crude and photo-degraded extracts of either sex. Examination of all volatile samples using gas chromatography coupled with mass spectrometry (GC-MS) revealed that most of the identified compounds in photo-degraded extracts were also present in crude extracts. However, the abundance of these compounds in photo-degraded samples were 10 to 250 times more than their abundance in the crude counterparts. Results from behavioral bioassays indicate that photo-degradation probably does not generate a long-range pheromone, but it may be involved in the production of a short-range sex-recognition pheromone in SLF. This study provides additional evidence of pheromonal activity in SLF. [ABSTRACT FROM AUTHOR]

Simple Summary: The EU has established ambitious goals for pesticide reduction. The achievement of these goals requires the introduction of easy-to-use, low-cost and effective tools to monitor crop pests. Sex pheromone YATLORf (Yf) traps have proven to be a reliable low-cost tool for monitoring Agriotes spp., which are the most harmful group of soil pests in Europe. They can damage a wide range of arable crops, including potato, maize and sunflower. In order to optimize Yf use, this study explored the effect of lure position in the trap and vegetation field cover density on trap performance. Lure attractant capacity varied greatly with the lure's position in the trap and the extent of the vegetation density around the trap. Guidelines for making practical decisions are given. The 'low' lure position in the Yf is suitable for all species in all field conditions and is the best choice for A. brevis; the 'medium' position should be considered for A. brevis only when vegetation is dense. The 'high' lure position is unsuitable for A. brevis and A. obscurus, and should be considered for some species only. Dense vegetation (e.g., winter wheat) reduced the potential for catching A. sordidus. Placing the trap just outside the field, or in a nearby field with bare/sparse vegetation, maintained the maximum catching potential. Vegetation density was found to influence beetle sex ratio, with no or very few female A. brevis and A. sordidus being found in traps placed in the middle of fields with dense vegetation. This research has made it possible to conduct studies on multi-baiting the same trap, which can significantly reduce monitoring costs. Low-cost monitoring tools are needed to implement IPM in arable crops. YATLORf (Yf) traps baited with respective synthetic pheromone lures have proven to be a reliable tool for monitoring Agriotes spp., Europe's most harmful soil pests. To optimize Yf use, we studied the effect of lure position in the trap and crop density on trap performance. Yf management detail was studied between 2000–2003 and 2014–2016 in various countries, with the traps being arranged in blocks. Each block contained one trap per treatment (i.e., lure position) under study. It was ascertained that lure attractant capacity can vary greatly with the lure's position in the trap and the extent of vegetation. Information for making practical decisions is given. The 'low' lure position is suitable for all species in all field conditions, and is the best choice for A. brevis. Lures for A. brevis and A. lineatus need to be placed in the low position when the field has no or sparse vegetation cover. The 'high' lure position is unsuitable for A. brevis and A. obscurus, and should be considered for some species only. There are no restrictions on position for catching A. sordidus, i.e., any position is suitable. Dense vegetation (e.g., wheat) reduced the Yf trap's potential for catching A. sordidus. Placing the trap just outside the field, or in a nearby field with bare/sparse vegetation, maintained the maximum catching potential. Vegetation density also influenced beetle sex ratio, with A. brevis and A. sordidus females always found in traps placed in fields with bare or low-density vegetation. Our findings have made it possible to obtain consistent monitoring outputs and to begin studies on multi-baiting the same trap, which can significantly reduce monitoring costs. [ABSTRACT FROM AUTHOR]

The pine sawfly Diprion jingyuanensis Xiao and Zhang (Hymenoptera: Diprionidae) is a serious pest of Pinus tabulaeformis Carr. in the Shanxi, Gansu, and Inner Mongolia provinces in P. R. China. The sex pheromone of D. jingyuanensis was shown to be the propionate ester of 3,7-dimethyl-2-tridecanol. Virgin females contained an approximate 1:3 blend of the pheromone precursors erythro-(2S,3S,7R/S and 2R,3R,7R/S)-3,7-dimethyl-2-tridecanol and threo-(2S,3R,7R/S and 2R,3S,7R/S)-3,7-dimethyl-2-tridecanol, but the exact stereoisomers were not determined. Males responded the strongest to the propionate ester of the two threo-isomers, (2S,3R,7R) and (2S,3R,7S), in electroantennogram (EAG) recordings, followed by a significant EAG response to the (2S,3R,7R) propionate of diprionol (pheromone component of D. similis), whereas the remaining two isomers (2S,3S,7S and 2S,3S,7R) of the propionate ester of 3,7-dimethyl-2-tridecanol and the acetate of the (2S,3R,7R) isomer (one of the two pheromone components of D. pini) did not elicit any significant increase in antennal response. In the field, the strongly EAG-active (2S,3R,7R)-isomer alone was only weakly (but significantly) attractive to D. jingyuanensis males at 100 µg, while the equally EAG- active (2S,3R,7S)-isomer alone at the same loading was 8–14 times more attractive than was the (2S,3R,7R)-isomer alone. Traps baited with the same amounts of the two threo-isomers ((2S,3R,7R) and (2S,3R,7S), 100 µg + 100 µg) caught significantly more males than did traps baited with other isomers, either of the two isomers alone or other proportions of the two isomers. Thus, the (2S,3R,7S)-isomer is considered as a strong and essential sex-attractant component for D. jingyuanensis males, whereas the (2S,3R,7R)-isomer is a weak but synergistic sex-attractant. This is one of the few examples of a pine sawfly responding significantly stronger to a binary blend of stereoisomers in a synergistic fashion than to a single stereoisomer alone. [ABSTRACT FROM AUTHOR]

Ant colony optimization (ACO) is a well-known class of swarm intelligence algorithms suitable for solving many NP-hard problems. An important component of such algorithms is a record of pheromone trails that reflect colonies' experiences with previously constructed solutions of the problem instance that is being solved. By using pheromones, the algorithm builds a probabilistic model that is exploited for constructing new and, hopefully, better solutions. Traditionally, there are two different strategies for updating pheromone trails. The best-so-far strategy (global best) is rather greedy and can cause a too-fast convergence of the algorithm toward some suboptimal solutions. The other strategy is named iteration best and it promotes exploration and slower convergence, which is sometimes too slow and lacks focus. To allow better adaptability of ant colony optimization algorithms we use κ-best, max-κ-best, and 1/λ-best strategies that form the entire spectrum of strategies between best-so-far and iteration best and go beyond. Selecting a suitable strategy depends on the type of problem, parameters, heuristic information, and conditions in which the ACO is used. In this research, we use two representative combinatorial NP-hard problems, the symmetric traveling salesman problem (TSP) and the asymmetric traveling salesman problem (ATSP), for which very effective heuristic information is widely known, to empirically analyze the influence of strategies on the algorithmic performance. The experiments are carried out on 45 TSP and 47 ATSP instances by using the MAX-MIN ant system variant of ACO with and without local optimizations, with each problem instance repeated 101 times for 24 different pheromone reinforcement strategies. The results show that, by using adjustable pheromone reinforcement strategies, the MMAS outperformed in a large majority of cases the MMAS with classical strategies. [ABSTRACT FROM AUTHOR]

Knowledge of the biology of a pest is essential for building sustainable management programmes. Pentatomidae have a hemimetabolous life cycle with egg, nymphal, and adult life stages, which differ in morphological, ecological, and behavioural traits. Some of these traits, such as mating behaviour, pheromones (alarm and aggregation pheromones) and the acquisition of gut symbionts can be targeted for pest management strategies. Here, we review the available literature on these life history traits of the Pentatomidae with potential for use in management programmes. Pheromone-mediated aggregation and the disruption of symbiont acquisition are two important targets for Pentatomidae control. Other traits such as the use of alarm pheromones for enhancing natural enemies and substrate-borne vibration for mating disruption deserve further consideration. Colour vision and flight ability are still poorly studied, despite their potential importance for stink bug management. [ABSTRACT FROM AUTHOR]

Traditional traps for Spodoptera frugiperda (J. E. Smith) monitoring require manual counting, which is time-consuming and laborious. Automatic monitoring devices based on machine vision for pests captured by sex pheromone lures have the problems of large size, high power consumption, and high cost. In this study, we developed a micro- and low-power pest monitoring device based on machine vision, in which the pest image was acquired timely and processed using the MATLAB algorithm. The minimum and maximum power consumption of an image was 6.68 mWh and 78.93 mWh, respectively. The minimum and maximum days of monitoring device captured image at different resolutions were 7 and 1486, respectively. The optimal image resolutions and capture periods could be determined according to field application requirements, and a micro-solar panel for battery charging was added to further extend the field life of the device. The results of the automatic counting showed that the counting accuracy of S. frugiperda was 94.10%. The automatic monitoring device had the advantages of low-power consumption and high recognition accuracy, and real-time information on S. frugiperda could be obtained. It is suitable for large-scale and long-term pest monitoring and provides an important reference for pest control. [ABSTRACT FROM AUTHOR]

Pheromones are chemical signals secreted by one individual that can affect the behaviors of other individuals within the same species. Ascaroside is an evolutionarily conserved family of nematode pheromones that play an integral role in the development, lifespan, propagation, and stress response of nematodes. Their general structure comprises the dideoxysugar ascarylose and fatty-acid-like side chains. Ascarosides can vary structurally and functionally according to the lengths of their side chains and how they are derivatized with different moieties. In this review, we mainly describe the chemical structures of ascarosides and their different effects on the development, mating, and aggregation of nematodes, as well as how they are synthesized and regulated. In addition, we discuss their influences on other species in various aspects. This review provides a reference for the functions and structures of ascarosides and enables their better application. [ABSTRACT FROM AUTHOR]

Simple Summary: Within the existing pheromone-based strategies for Lobesia botrana monitoring and management, mating disruption appears to be the most studied and deployed in wine-growing contexts worldwide. The effectiveness of this strategy led to many efforts to improve it, relying upon new available technologies. In this study, we assessed the efficacy of a novel active pheromone emitter (product code: Isonet® L MISTERX843) for L. botrana mating disruption, testing it at three different densities (i.e., 2, 3 and 4 units/ha). The aerosol emitter was evaluated in two wine-growing contexts, where it significantly reduced the L. botrana infestation when compared to vineyards not managed with mating disruption. The aerosol efficacy performances were comparable to those obtained with passive dispensers (Isonet® L TT) and another active dispenser (Checkmate® Puffer® LB) already on the market. Overall, this novel aerosol device ensures the high effectiveness of the mating disruption program. On the other hand, extra care should be given for their deployment in the field, considering many factors such as the orography and shape of the vineyard as well as the dominant winds. Despite the great amount of information on the European Grapevine Moth (EGVM), Lobesia botrana (Lepidoptera: Tortricidae), and the effective strategies available for its management, this moth remains the main key pest damaging grapevines in the Mediterranean and Central Europe wine-growing areas. Synthesizing and manipulating its sex pheromone components fostered the development of new dispensers to boost the effectiveness and sustainability of mating disruption (MD) programs. Recent MD research has highlighted that the effectiveness of aerosol emitters is comparable to that of passive dispensers when applied in large, uniform sites such as Spanish vineyards. However, aerosol emitters that are equally effective in geographical areas characterized by small-sized vineyards, typical of many Italian regions, have not received enough research attention. To face this challenge, herein the experimental aerosol emitter (product code: Isonet® L MISTERX843) was tested at three different application rates (i.e., 2, 3 and 4 units/ha) in three study sites, two in Tuscany (Central Italy in 2017 and 2018) and one in Emilia-Romagna (Northern Italy in 2017), respectively, for a total of five trials. To assess the efficacy of this novel MD aerosol emitter, three different application densities were compared with an untreated control and two grower's standards. The latter were represented by passive (Isonet® L TT) and active (Checkmate® Puffer® LB) release dispensers, already on the market for EGVM MD and applied at, respectively, 200–300 and 2.5–4 units/ha. MD carried out with Isonet® L MISTERX843 led to zero catches of males in the pheromone traps. They also allowed for a significant reduction in the number of infested flower clusters and bunches, as well as in the number of nests per flowers cluster/bunch, if compared to the untreated control. As a general trend, MD effectiveness was fully comparable, or even better, if compared to the grower's standard. In conclusion, our research pointed out that the Isonet® L MISTERX843 can allow for effective EGVM management in small-sized Italian vineyards. Lastly, our economic evaluation showed that the MD whole cost per hectare using active or passive release devices was comparable. [ABSTRACT FROM AUTHOR]

Simple Summary: Over the past two decades, researchers around the world have conducted studies to identify volatile compounds that serve as attractants for one of the most important agricultural pests, the western flower thrips (WFT). In this review, the preferred reporting items for systematic reviews and meta-analyses (PRISMA) guidelines were used to find published studies and to extract the information generated worldwide to determine the overall attraction of the reported compounds using a meta-analysis approach. This systematic review shows that the most-used compounds worldwide are those based on pyridines, which show a high attraction ratio. Methyl isonicotinate is the most studied of these compounds. Data from the meta-analysis accurately estimated the potential attraction of other novel compounds that could be used for integrated pest management strategies, such as monitoring WFT populations or mass trapping. A call for new research evaluating and developing new products with these novel compounds is made. The study of the semiochemicals of the western flower thrips (WFT), Frankliniella occidentalis, Pergande (Thysanoptera: Thripidae), is a relevant topic that spans the last two decades. Approximately a hundred articles published on this subject from 2000 to 2022 can be found in academic databases, representing approximately 5% of the research on this important pest. These topics have generated a platform for novel research with a high potential for development. However, to move on to a new research step, an effectiveness evaluation of the compounds discovered so far is necessary. This review conducted a systematic analysis of the research focused on the semiochemicals (kairomones, pheromones, and attractants) for this pest. Papers from the past three decades on WFT attraction to semiochemicals were collected from databases using the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. The number of individuals attracted to compounds was extracted from the papers and compiled for analysis. With this information, an attraction ratio was calculated. Forty-one possible attractants were found in the literature, with methyl isonicotinate being the most-studied compound so far, with the third-highest attraction ratio. δ-Decalactone was the compound with the highest attraction ratio, but it was one of the least studied. A meta-analysis of the WFT choosing proportion was performed for the compounds with more trials found in the literature. The predicted mean choice percentages for methyl isonicotinate (MIN) and Lurem-TR, the MIN's commercial product, were 76.6% and 66.6%, respectively. There was a convergence among the analyzed studies showing a high degree of research focus on the same group of nitrogen-containing compounds (mainly the pyridine structure). These findings call for future research to diversify the discovery and evaluation of attractive compounds in this relevant study area. [ABSTRACT FROM AUTHOR]

Tuta absoluta (Meyrick), a leafminer that damages tomato leaves, terminal buds, flowers, and fruits, is a destructive tomato pest and is responsible for 80–100% of tomato yield losses globally. Different insect species have different courtship responses and phototropic flight rhythms. Improving the trapping effects of the sex pheromone and light traps is important for constructing an IPM system for T. absoluta. The present study explored the hourly distribution of T. absoluta adults caught by the sex pheromone (on the ground) and UV light (380 nm) traps in greenhouses over 24 h. The responses of males to sex pheromone (false female) lures were detected at dawn and early morning. The responses lasted for 3 h, from 05:30 (1 h before sunrise) to 08:30 (2 h after sunrise), and 95.8% of the males were caught during this period. The peak of the male responses to the sex pheromone was detected at 07:30 (from 06:30 to 07:30, 1 h after sunrise), and 80.8% of the males were caught during this period. The flight of male (proportion of 54.3%) and female (45.7%) adults toward the UV light traps occurred from 19:30 (time of sunset) to 06:30 (time of sunrise), lasted for 11 h, and exhibited a scotophase rhythm; 97.4% of the adults were caught during this period. The peak of adults flying toward the UV light traps occurred at 21:30 (from 20:30 to 21:30, 2 h after sunset). The rhythms of males' responses to the sex pheromone and of the adults' flight toward the UV lights can help to reveal the mechanisms of chemotactic and phototactic responses and may play a significant role in constructing an IPM system for this pest. [ABSTRACT FROM AUTHOR]

Simple Summary: During the breeding process of mice, the females can evaluate the health status of males and choose the suitable mates through the smell of urine. Under the natural conditions, parasitic infections in small mammals are very common, and parasitic infections can affect the health status of hosts. However, the impact of parasitic infection on mammalian reproductive capacity has not been fully studied. In this study, we used the ICR/CD-1 mouse model to explore the relationship between parasitic infection and pheromone content; Cryptosporidium parvum was used as the infectious agent. This study revealed that the urine attractiveness of the ICR/CD-1 male mice was decreased after parasitic infection when compared with the uninfected group. Both the gene expression level and protein concentration of MUP in liver and urine were down-regulated after parasitic infection. The results of GC-MS analysis indicated that the contents of different pheromones were significantly reduced after parasitic infection. In summary, this study revealed that infection with C. parvum affects the secondary sexual characteristics of male ICR/CD-1 mice and alters the pheromone contents in the foreskin gland. The olfactory acuity of female mice allows them to discriminate the urinary odors of males. Parasitic infection can reduce the odor attractiveness of male mice to females and result in female aversion or avoidance responses in odor selection. However, the chemical signaling changes in the pheromone contents produced by the foreskin gland were not fully revealed after parasitic infection. Cryptosporidium parvum (C. parvum) is a common zoonotic intestinal parasite and has a wide range of hosts, including human, domestic animals, and wild animals. In this study, we immunosuppressed ICR/CD-1 male mice by dexamethasone sodium phosphate treatment. After C. parvum infection, physiological indexes such as body weight and organ weight were significantly decreased. Furthermore, the gene expression level of MUP (major urinary protein) in liver and urine were significantly down-regulated, which could be the reason for the decrease in urine attractiveness to females. GC-MS was performed to analyze the changes in the pheromone produced by the preputial gland before and after parasitic infection, and the results indicated that the levels of different pheromones were significantly reduced after parasitic infection. In summary, this study reveals that C. parvum infection damages the secondary sexual characteristics of male ICR/CD-1 male mice and decreases the pheromone content produced by the foreskin gland. [ABSTRACT FROM AUTHOR]

Simple Summary: The invasive Asian longicorn beetle Anoplophora glabripennis and native Japanese white-spotted longicorn beetle A. malasiaca show an extensive overlap with host plants, niches, and emergence season. Hybridization between these two species is suspected. The surface of the female body is covered with contact sex pheromones that elicit male mating behavior within species. We evaluated the contact pheromonal activity of crude extract and fractions of female A. glabripennis and revealed a hydrocarbon fraction to show activity; however, it was relatively weak. There might be other, as yet unidentified active compounds in the extract. Only a small number of male A. glabripennis showed mating behavior when they were exposed to the crude extract of female A. malasiaca, whereas a considerable number of male A. malasiaca showed mating behavior when exposed to both female A. glabripennis and A. malasiaca extracts. Gomadalactones are essential contact pheromone components that elicit mating behavior in male A. malasiaca. We, therefore, attempted to detect gomadalactones in female A. glabripennis extract, but without success. We investigated the possible reasons for this phenomenon and the difference in male mate recognition systems between A. glabripennis and A. malasiaca. The Asian longicorn beetle Anoplophora glabripennis is a recently arrived invasive species to Japan. The Japanese native A. malasiaca shows an extensive overlap with A. glabripennis with host plants, niches, and emergence season. Hybridization between these two species is suspected in Japan. The surface of the female is covered with contact sex pheromones that elicit male mating behavior within species. We evaluated the contact pheromonal activity of crude extract and fractions of female A. glabripennis coated on a black glass model and revealed a hydrocarbon fraction and a blend of fractions to show activity but relatively weak, suggesting the presence of other unknown active compounds. Few male A. glabripennis showed mating behavior when they were exposed to a crude extract of female A. malasiaca. However, a considerable number of A. malasiaca males mounted and showed abdominal bending behavior when presented with glass models that were coated with each extract of female A. glabripennis and A. malasiaca. Gomadalactones are essential contact pheromone components that elicit mating behavior in male A. malasiaca; however, we could not detect them in female A. glabripennis extract. Here, we investigated the possible reasons for this phenomenon and the difference in male mate recognition systems between these two species. [ABSTRACT FROM AUTHOR]

Simple Summary: The brown marmorated stink bug, Halyomorpha halys, is an invasive insect pest native to Asia that was accidently introduced into North America and Europe. The male-produced aggregation pheromone of H. halys has great application potential in population monitoring and control of this pest. In this study, we carried out a comprehensive bioinformatics analysis to identify the candidate genes involved in the biosynthesis of the male-specific sesquiterpene aggregation pheromone of H. halys. We identified a novel gene, designated as HhTPS1, which is related to the synthesis of the precursor molecule of the aggregation pheromone. Moreover, potential P450 reductase and transcription factors related to the aggregation pheromone biosynthesis pathway were detected. In addition, two olfactory-related genes, chemosensory protein 5 (HhCSP5) and olfactory receptor 85b (HhOr85b), were also identified, which are assumed to be involved in the olfactory recognition of this pheromone. Our results lay a solid foundation for further elucidating the biosynthesis of this aggregation pheromone and its behavioral regulation of H. halys. The aggregation pheromone of the brown marmorated stink bug, Halyomorpha halys (Stål), is produced by adult males, and plays an important role in the behavioral regulation of H. halys. However, information on the molecular mechanisms underlying this pheromone's biosynthesis is limited. In this study, HhTPS1, a key candidate synthase gene in the aggregation pheromone biosynthesis pathway of H. halys, was identified. Then, through weighted gene co-expression network analysis, the candidate P450 enzyme genes in the biosynthetic downstream of this pheromone and the related candidate transcription factor in this pathway were also identified. In addition, two olfactory-related genes, HhCSP5 and HhOr85b, involved in the recognition of the aggregation pheromone of H. halys, were detected. We further identified the key amino acid sites of HhTPS1 and HhCSP5 that interact with substrates by using molecular docking analysis. This study provides basic information for further investigations into the biosynthesis pathways and recognition mechanisms of aggregation pheromones in H. halys. It also provides key candidate genes for bioengineering bioactive aggregation pheromones necessary for the development of technologies for the monitoring and control of H. halys. [ABSTRACT FROM AUTHOR]

Simple Summary: Up to date, little is known about the role of semiochemicals in cheetahs. Scientists have speculated that the concentration of VOCs potentially involved in breeding behaviour could be present at low concentrations since cheetahs' urine emits very little odour. Therefore, the effect of a synthetic scent has been tested on female cheetahs (Acinonyx jubatus) behaviour with the objective of possibly increasing the frequency of reproductive (e.g., precopulatory and copulatory) behaviour displayed by female cheetahs. Furthermore, this study aimed at investigating the function of semiochemicals in reproduction in this species. The study indicates that volatile organic chemicals (VOCs) have an effect on cheetahs and their behaviour; however, additional research is required to determine the effects of specific VOCs on cheetah reproduction. "Fine tuning" of the synthetic scent could potentially aid in improving captive breeding, as well as preventing asymmetric reproductive ageing. In cheetahs, age at first parturition correlates negatively with reproductive lifespan (asymmetric reproductive aging); therefore, breeding cheetahs at a young age is essential to maximize reproductive performance in this species. However, younger females display a significantly reduced frequency of copulatory behaviour, which negatively affects breeding. Volatile organic compounds (VOCs) are known to regulate appropriate behavioural responses in various species, including reproductive behaviour; moreover, they have proven to play a role in captive breeding methods in cheetahs, as well as mate choice. Therefore, the objective of this study was to evaluate the effect of a synthetic scent (SS) on the frequency of the five oestrous behaviour(s) (sniff, rub, roll, spray, and meow-chirp) known to be indicative of oestrus in female cheetahs. Based on the results of a previous study from our research group, five VOCs, identified in the marking fluid of male cheetahs, and known to be pheromones involved in reproductive behaviour, were used to create the SS. This was accomplished by mixing benzaldehyde, acetophenone, indole, dimethyl disulphide and phenol with (99.9%) ethanol. Seven female cheetahs were then observed for one oestrus cycle without stimulation (control) and then once again while exposed to the SS (treatment), which was sprayed on foil trays placed around the outside of each enclosure. The occurrence of the five oestrous behaviours was recorded and tallied per day of observations. Although the SS did not have a significant effect on the frequency of oestrous behaviours displayed by the females used in this study, five of the seven (71%) did show an increase in their behaviour with the SS when oestrogen concentrations were at their highest (peak oestrus), including three of the four younger females. The SS also significantly increased the sniffing behaviour in general. Although the results of this study do indicate that VOCs influence cheetahs and their behaviour, firm conclusions cannot be drawn due to the low number of animals used, as well as the significant effect the observation methods had on the results. Nonetheless, this study represents the first of this kind in cheetahs, therefore representing an important step in determining the role of VOCs in aiding breeding in captivity. [ABSTRACT FROM AUTHOR]