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Pemphigus is an autoimmune disease that affects the skin and mucous membranes, induced by the deposition of pemphigus IgG, which mainly targets desmogleins 1 and 3 (Dsg1 and 3). This autoantibody causes steric interference between Dsg1 and 3 and the loss of cell adhesion, producing acantholysis. This molecule and its cellular effects are clinically reflected as intraepidermal blistering. Pemphigus vulgaris-IgG (PV-IgG) binding involves p38MAPK-signaling-dependent caspase-3 activation. The present work assessed the in vitro effect of PV-IgG on the adherence of HaCaT cells dependent on caspase-3. PV-IgG induced cell detachment and apoptotic changes, as demonstrated by annexin fluorescent assays. The effect of caspase-3 induced by PV-IgG was suppressed in cells pre-treated with caspase-3-shRNA, and normal IgG (N-IgG) as a control had no relevant effects on the aforementioned parameters. The results demonstrated that shRNA reduces caspase-3 expression, as measured via qRT-PCR and via Western blot and immunofluorescence, and increases cell adhesion. In conclusion, shRNA prevented in vitro cell detachment and the late effects of apoptosis induced by PV-IgG on HaCaT cells, furthering our understanding of the molecular role of caspase-3 cell adhesion dependence in pemphigus disease. [ABSTRACT FROM AUTHOR]

This literature review explores cutting-edge microencapsulation techniques designed to enhance the antimicrobial efficacy of essential oils in dairy products. As consumer demand for natural preservatives rises, understanding the latest advancements in microencapsulation becomes crucial for improving the shelf life and safety of these products. The bibliometric analysis utilized in this review highlighted a large number of documents published on this topic in relation to the following keywords: essential oils, AND antimicrobials, AND dairy products, OR microencapsulation. The documents published in the last 11 years, between 2013 and 2023, showed a diversity of authors and countries researching this topic and the keywords commonly used. However, in the literature consulted, no study was identified that was based on bibliometric analysis and that critically evaluated the microencapsulation of essential oils and their antimicrobial potential in dairy products. This review synthesizes findings from diverse studies, shedding light on the various encapsulation methods employed and their impact on preserving the quality of dairy goods. Additionally, it discusses the potential applications and challenges associated with implementation in the dairy industry. This comprehensive analysis aims to provide valuable insights for researchers, food scientists, and industry professionals seeking to optimize the use of essential oils with antimicrobial properties in dairy formulations. [ABSTRACT FROM AUTHOR]

The diverse morphological configurations in teeth present clinical challenges in root canal treatment, complicating instrumentation and irrigation processes, which can lead to treatment failure. Understanding anatomical variations, such as C-shaped canals and radix entomolaris, enhances clinical skills and improves long-term endodontic treatment success rates. Cone-beam computed tomography (CBCT) offers superior diagnostic capabilities over conventional radiography, enabling the pre-operative detection of root configurations and canal numbers, facilitating personalized endodontic treatments. A total of 2173 teeth of a Mexican population, including 1057 first mandibular molars and 1116 s mandibular molars, were studied using only CBCT to identify C-shaped canals and radix configurations of patients who were treated from 2018 to 2023 at the Department of Radiology at the Faculty of Dentistry, Juarez University of the State of Durango, Mexico. C-shaped canals were identified in 160 teeth, with a prevalence of 0.2% in first mandibular molars and 14.1% in second mandibular molars. The highest frequency was in the left second mandibular molar (3.7) at 14.8%. Gender differences were significant, with higher prevalence in females (27.3%) compared to males (13.3%). The most common C-shaped canal configuration was type C2 (39.3%). Radix entomolaris was found in 52 teeth, with a prevalence of 3.4% in first mandibular molars and 1.4% in second mandibular molars. This research on a Mexican population using cone-beam computed tomography (CBCT) highlights significant findings in the prevalence and types of C-shaped canals and radix entomolaris in mandibular molars for this population. The left second mandibular molar (3.7) showed the highest prevalence at 14.8%, followed closely by the right second mandibular molar (4.7) at 13.5%, with a significant difference (p < 0.001). We found a significant difference in the prevalence of C-shaped canals between genders (p = 0.004, OR 1.78). Additionally, radix entomolaris (p < 0.001) was more frequently identified in first mandibular molars to a significant degree. These insights underscore the importance of CBCT in diagnosing complex root anatomies, which can greatly enhance the success rates of endodontic procedures by allowing for more tailored and precise treatments for this population. [ABSTRACT FROM AUTHOR]

This study aimed to validate a semiquantitative electronic food frequency questionnaire (eFFQ) in estimating the intake of a comprehensive list of nutrients and bioactive compounds among adults from six regions of Argentina using multiple 24 h dietary recall (24HR) as a reference. A total of 163 adults completed two administrations of the eFFQ and four 24HRs. The paired t-test/Wilcoxon signed-rank test, Spearman/Pearson correlations, cross-classification, weighted kappa statistics, and Bland–Altman plots were employed to determine relative validity. To determine reproducibility, intraclass correlations (ICC), cross-classification, and weighted kappa statistics were calculated. For relative validity, crude correlations ranged from 0.15 to 0.57; energy adjustment and de-attenuation slightly improved most of these correlations. In cross-classification analysis, agreements within one quintile adjacent to exact agreement (EA ± 1) ranged from 52.2% to ~74%; extreme misclassifications were < 7%. For reproducibility, the crude ICC ranged from 0.29 to 0.85, showing moderate to good correlations for most nutrients. Cross-classification analysis showed agreement levels for the EA ± 1 quintile of 70.6% to 87.7%. Weighted kappa values ranged from 0.21 to 0.62. The results show that this eFFQ is relatively valid in ranking adults according to their nutrient intake and has an acceptable reproducibility, yet it slightly overestimates the intake of most nutrients. [ABSTRACT FROM AUTHOR]

The expansion of food production is becoming more important due to a rising world population, which is relying on food security on regional and local scales. Intensive food production systems exert a negative impact on the regional ecosystem because of agrochemical pollution and nutrient-rich water discharging into nearby rivers. Furthermore, these systems highly depend on regional water resources, causing water scarcity and soil erosion due to the overexploitation of natural resources in general. The objective of this article is to review the water usage in the two most water-intensive food production systems, agriculture and aquaculture, showing lacking areas like system management and climate change, which must be considered in the implementation of a sustainable water footprint. In addition, the review includes an analysis of the combination of both production systems in aquaponic food production and the possibilities of water saving. There are a variety of analyses related to water usage for crop and aquatic animal production, but in these analyses, there is a lack of information about system management in general, which includes cleaning processes, water substitution, pond removal, water evaporation, and, especially in aquaculture, the water usage required for industrially elaborated fish feed. [ABSTRACT FROM AUTHOR]

The rise in non-albicans Candida species, exhibiting unpredictable antifungal resistance, complicates treatment and contributes to the growing threat of invasive, life-threatening infections. This study evaluates the antifungal activity of four benzo[a]phenoxazine derivatives (C34, C35, A42, and A44) against 14 Candida strains following EUCAST standards. Fluconazole interactions are analysed through fractional inhibitory concentration index (FICI) calculation and response surface analysis based on the Bliss model. Macrophage-like J774A.1 cells are used to assess Candida killing in the presence of synergistic compounds. The MIC values against the different strains vary, with C34 showing the strongest activity, followed by C35, while A42 has the highest MIC values, indicating lower efficacy. However, A42 demonstrates the best synergy with fluconazole against fluconazole-resistant Candida strains. Cytotoxicity assays reveal that the chloropropyl group present in C35 and A42 enhances cytocompatibility. Co-culture with macrophages shows significant yeast killing for C. albicans and C. auris when fluconazole and A42 are combined, requiring concentrations 4 and 16 times lower than their MIC values, enhancing antifungal activity. Given fluconazole's fungistatic nature and the emergence of drug-resistant strains, benzo[a]phenoxazine derivatives' ability to enhance fluconazole's efficacy present a promising strategy to address antifungal resistance in critical pathogens. These findings align with global research priorities, offering new potential avenues for developing more effective antifungal therapies. [ABSTRACT FROM AUTHOR]

Variability in manufacturing processes must be properly monitored and controlled to avoid incurring quality problems; otherwise, the probability of manufacturing defective products increases, and, consequently, production costs rise. This paper presents the development of a methodology to locate the source(s) of variation in the manufacturing process in case of a statistical deviation so that the user can quickly take corrective actions to eliminate the source of variation, thus avoiding the manufacture of out-of-specification products. The methodology integrates the multivariate cumulative sum control chart and the multilayer perceptron artificial neural network for the detection and interpretation of the source(s) of variation generated in the manufacturing processes. A case study was carried out with a printed circuit board manufacturing process, and it was possible to classify the origin of the variation with a sensitivity of 92.41% and specificity of 91.16%. The results demonstrate the viability of the proposed methodology to monitor and interpret the source of statistical variation present in production systems. [ABSTRACT FROM AUTHOR]

Background: This review investigates the impact of bioactive molecules produced by probiotics on child health, focusing on their roles in modulating gut microbiota, enhancing immune function, and supporting overall development. Key metabolites, including short-chain fatty acids (SCFAs), bacteriocins, exopolysaccharides (EPSs), vitamins, and gamma-aminobutyric acid (GABA), are highlighted for their ability to maintain gut health, regulate inflammation, and support neurodevelopment. Objectives: The aim of this review is to examine the mechanisms of action and clinical evidence supporting the use of probiotics and postbiotics in pediatric healthcare, with a focus on promoting optimal growth, development, and overall health in children. Methods: The review synthesizes findings from clinical studies that investigate the effects of probiotics and their metabolites on pediatric health. The focus is on specific probiotics and their ability to influence gut health, immune responses, and developmental outcomes. Results: Clinical studies demonstrate that specific probiotics and their metabolites can reduce gastrointestinal disorders, enhance immune responses, and decrease the incidence of allergies and respiratory infections in pediatric populations. Additionally, postbiotics—bioactive compounds from probiotic fermentation—offer promising benefits, such as improved gut barrier function, reduced inflammation, and enhanced nutrient absorption, while presenting fewer safety concerns compared to live probiotics. Conclusions: By examining the mechanisms of action and clinical evidence, this review underscores the potential of integrating probiotics and postbiotics into pediatric healthcare strategies to promote optimal growth, development, and overall health in children. [ABSTRACT FROM AUTHOR]

Background/Objectives: Twenty-four-hour movement behaviors, including physical activity, sedentary time, and sleep, are associated with a range of health outcomes. However, the relationship between the combinations of these behaviors and dietary outcomes in young populations remains unclear. We conducted a systematic review to explore how combined movement behaviors are linked to dietary outcomes in children and adolescents. Methods: We searched MEDLINE, Embase, and PsycINFO from January 2017 to July 2024, including studies that were (1) written in English, (2) published in peer-reviewed journals, (3) quantitatively analyzed two or three movement behaviors with dietary outcomes, and (4) focused on populations aged between 0 and 18 years. Results: Ten cross-sectional studies met these criteria. The majority of studies (6 out of 10) assessed all three movement behaviors, although sleep was less frequently examined, appearing in only 4 of the studies. Our findings indicated that adhering to all movement recommendations was consistently associated with healthier dietary patterns, including a higher consumption of fruits, vegetables, nuts, fish, and cereals, and a lower intake of sweets and pastries. Additionally, adhering to physical activity and sedentary time guidelines was linked to improved dietary habits in children, including more fruit and vegetable intake and less sugary drink and snack consumption. Conversely, higher sedentary time and inadequate sleep were linked to poorer dietary outcomes, particularly lower fruit intake and hydration. Conclusions: This review suggests that meeting movement behavior guidelines correlates with healthier dietary outcomes in children and adolescents. However, given the limited number of studies and their cross-sectional design, further high-quality research, including longitudinal and intervention studies, is needed to clarify causal relationships. [ABSTRACT FROM AUTHOR]

Rural communities in Oaxaca face challenges such as food insecurity, economic instability, and the loss of traditional agricultural knowledge. Home gardens, once essential for food production and income generation, have declined due to migration, environmental degradation, and modern agricultural pressures. This study evaluated how community-driven models, supported by crowdfunding, can revitalize home gardens to improve food security, build community resilience, and promote economic empowerment. A mixed-methods approach, including household surveys, interviews, and focus groups, was used to assess the impact of these gardens. The results showed that over 70% of households experienced improved food security and reduced reliance on external food sources. Home garden production also led to a 20% reduction in food expenses, and the sale of surplus crops provided additional income, enhancing household economic stability. Women's involvement in agricultural decision-making increased, fostering empowerment within both family and community contexts. Traditional practices, such as seed saving and organic pest control, were maintained, contributing to the ecological sustainability of the gardens. Crowdfunding was an effective tool for financing home gardens, boosting community engagement and strengthening social bonds. The findings highlight the potential of integrating traditional knowledge with modern funding strategies to create resilient, sustainable agricultural systems in rural Oaxaca. [ABSTRACT FROM AUTHOR]

Vascular wilt is an important tomato disease that affects culture yields worldwide, with Fusarium oxysporum (F.o) being the causal agent of this infection. Several management strategies have lost effectiveness due to the ability of this pathogen to persist in soil and its progress in vascular tissues. However, nowadays, research has focused on understanding the plant defense mechanisms to cope with plant diseases. One recent and promising approach is the use of extracellular DNA (eDNA) based on the ability of plants to detect their self-eDNA as damage-associated molecular patterns (DAMPs) and pathogens' (non-self) eDNA as pathogen-associated molecular patterns (PAMPs). The aim of this work was to evaluate the effect of the eDNA of F.o (as a DAMP for the fungus and a PAMP for tomato plants) applied on soil, and of tomato's eDNA (as a DAMP of tomato plants) sprayed onto tomato plants, to cope with the disease. Our results suggested that applications of the eDNA of F.o (500 ng/µL) as a DAMP for this pathogen in soil offered an alternative for the management of the disease, displaying significantly lower disease severity levels in tomato, increasing the content of some phenylpropanoids, and positively regulating the expression of some defense genes. Thus, the eDNA of F.o applied in soil was shown to be an interesting strategy to be further evaluated as a new element within the integrated management of vascular wilt in tomato. [ABSTRACT FROM AUTHOR]

Chronic disease inflammation requires safe complementary treatments. The pericarp of Passiflora tripartita var. mollissima (PTM) contains potential anti-inflammatory metabolites. This study aimed to evaluate the bioactive components, antioxidant capacity, and anti-inflammatory effects of PTM extracts at two ripening stages. The bioactive compounds in the hydrophilic and lipophilic extracts of mature and green pericarps were identified by GC-MS and UV–VIS, while the antioxidant capacity was measured by free radical reduction. Anti-inflammatory effects were tested using a rat paw edema model with carrageenan-induced edema, indomethacin, or PTM extracts (100, 250, and 500 mg/kg). The effect of mature hydrophilic extract was further evaluated in an air pouch model, where rats received the placebo, carrageenan, indomethacin, or the extract (500 and 1000 mg/kg). Leukocytes, cytokines, and markers of oxidative stress were evaluated. The results showed the presence of organic compounds, total phenols, and flavonoids. The mature hydrophilic extract exhibited the highest antioxidant activity. At 500 mg/kg, it reduced edema, leukocyte migration, and levels of IL-1β, IL-6, and TNF-α while managing oxidative stress and preventing histological damage. In conclusion, PTM contains bioactive compounds with potential pharmacological properties. The hydrophilic extract of the mature pericarp, at a dose of 500 mg/kg, exhibits an enhanced antioxidant and anti-inflammatory effect. [ABSTRACT FROM AUTHOR]

The multifunctionality feature of graphene field-effect transistors (GFETs) is exploited here to design circuit building blocks of high-data-rate modulators by using a physics-based compact model. Educated device performance projections are obtained with the experimentally calibrated model and used to choose an appropriate improved feasible GFET for these applications. Phase-shift and frequency-shift keying (PSK and FSK) modulation schemes are obtained with 0.6   G Hz GFET-based multifunctional circuits used alternatively in different operation modes: inverting and in-phase amplification and frequency multiplication. An adequate baseband signal applied to the transistors' input also serves to enhance the device and circuit performance reproducibility since the impact of traps is diminished. Quadrature PSK is also achieved by combining two GFET-based multifunctional circuits. This device circuit co-design proposal intends to boost the heterogeneous implementation of graphene devices with incumbent technologies into a single chip: the baseband pulses can be generated with CMOS technology as a front end of line and the multifunctional GFET-based circuits as a back end of line. [ABSTRACT FROM AUTHOR]

In general, young plants in the establishment phase demonstrate sensitivity to changes in environmental conditions, especially regarding water availability. The effects of the seasonality of biophysical processes on plant physiology can trigger differential responses, even within the same region, making it necessary to conduct studies that characterize the physiological performance of the species at different spatial and temporal scales, making it possible to understand their needs and growth limits under water stress conditions. This paper aimed to evaluate the growth, gas exchange and Chl a fluorescence in ipê-rosa seedlings subjected to levels of water replacement (LWRs) of 100, 75, 50 and 25% in a greenhouse. The morphometric variables of plant height, diameter at stem height, numbers of leaves and leaflets, root length and volume, plant dry mass and leaf area were evaluated. The potential evapotranspiration of seedlings (ETc) was obtained using direct weighing, considering the water replacement of 100% of the mass variation between subsequent days as a reference; the cultivation coefficients (kc) were obtained using the ratio between ETc and the reference evapotranspiration (ETo) obtained by the Penman–Monteith FAO-56 method. Biomass and evapotranspiration data were combined to determine water sensitivity. Diurnal fluxes of gas exchange (net photosynthesis rate, transpiration rate, stomatal conductance, internal and atmospheric carbon ratio, water use efficiency and leaf temperature) and Chl a fluorescence (Fv/Fm, ΦPSII, ETR, Fv′/Fm′, NPQ and qL) were evaluated. Water restriction caused reductions of 90.9 and 84.7% in the increase in height and diameter of seedlings subjected to 25% water replacement when compared to seedlings with 100% water replacement. In comparison, biomass accumulation was reduced by 96.9%. The kc values increased throughout the seedling production cycle, ranging from 0.59 to 2.86. Maximum water sensitivity occurred at 50% water replacement, with Ky = 1.62. Maximum carbon assimilation rates occurred in the morning, ranging from 6.11 to 12.50 µmol m−2 s−1. Ipê-rosa seedlings regulate the physiology of growth, gas exchange and Chl a fluorescence depending on the amount of water available, and only 25% of the water replacement in the substrate allows the seedlings to survive. [ABSTRACT FROM AUTHOR]

Chronic noncommunicable diseases (NCDs) are responsible for approximately 74% of deaths globally. Medicinal plants have traditionally been used to treat NCDs, including diabetes, cancer, and rheumatic diseases, and are a source of anti-inflammatory compounds. This study aimed to evaluate the anti-inflammatory effects of Rhus trilobata (Rt) extracts and fractions in lipopolysaccharide (LPS)-induced inflammation models in vitro and in vivo. The aqueous extract (RtAE) and five fractions (F2 to F6) were obtained via C18 solid-phase separation and tested in murine LPS-induced J774.1 macrophages. Key inflammatory markers, such as IL-1β, IL-6, TNF-α, and COX-2 gene expression were measured using RT-qPCR, and PGE2 production was assessed via HPLC-DAD. The in vivo effects were tested in an LPS-induced paw edema model in Wistar rats. Results showed that RtAE at 15 μg/mL significantly decreased IL-1β and IL-6 gene expression in vitro. Fraction F6 further reduced IL-1β, TNF-α, and IL-6 gene expression, COX-2 expression, and PGE2 production. In vivo, F6 significantly reduced LPS-induced paw edema, inflammatory infiltration, and IL-1β and COX-2 protein expression. Chemical characterization of F6 by UPLC/MS-QTOF revealed at least eight compounds with anti-inflammatory activity. These findings support the anti-inflammatory potential of RtAE and F6, reinforcing the medicinal use of Rt. [ABSTRACT FROM AUTHOR]

Background/Objectives: The tibial nerve, commonly misnamed the "posterior tibial nerve", branches into four key nerves: the medial plantar, lateral plantar, inferior calcaneal (Baxter's nerve), and medial calcaneal branches. These nerves are integral to both the sensory and motor functions of the foot. Approximately 15% of adults with foot issues experience heel pain, frequently stemming from neural origins, such as tarsal tunnel syndrome (TTS). TTS diagnosis remains challenging due to a high false negative rate in neurophysiological studies. This study aims to improve the understanding and diagnosis of distal tarsal tunnel pathology to enable more effective treatments, including platelet-rich plasma, hydrodissections, radiofrequencies, and prolotherapy. Methods: Ultrasound-guided techniques were employed to examine the distal tarsal tunnel using the Heimkes triangle for optimal probe placement. Results: The results indicate that the tunnel consists of two chambers separated by the interfascicular septum, housing the medial, lateral plantar, and inferior calcaneal nerves. Successful interventions depend on precise visualization and patient positioning. This study emphasizes the importance of avoiding the calcaneus periosteum to reduce discomfort. Conclusions: Standardizing nerve involvement classification in TTS is difficult without robust neurophysiological studies. The accurate targeting of nerve branches is essential for effective treatment. [ABSTRACT FROM AUTHOR]

Introduction: Severe acute respiratory infection (SARI) is mainly caused by viral pathogens, with a high prevalence in high-risk populations such as infants and older adults. Coinfections by different viruses are frequent and, in some cases, associated with severe disease outcomes. Purpose: The main purpose of this study was to identify respiratory viruses circulating in Barranquilla during the peaks of the COVID-19 pandemic and estimate the prevalence of viral coinfections in samples from individuals with different degrees of respiratory infection. Methods: We received 5083 samples between epidemiological weeks 33–42 of 2021 submitted by the District Health Laboratory of Barranquilla and four local healthcare institutions during COVID-19 surveillance. Among them, we analyzed 101 samples from individuals presenting with influenza-like illness (ILI). Eighteen respiratory viruses, including SARS-CoV-2, were evaluated via qRT-PCR using nasal swabs or nasopharyngeal aspirate samples. Results: Of the 101 study individuals, 56 were male and 45 were female (55.5% and 44.5%, respectively); 25.7% of individuals were infected with at least one of the evaluated viruses. Respiratory syncytial virus (RSV) and human rhinovirus (HRV) were the two most frequently detected viruses (30.7% and 15.4% of total positives, respectively). Coinfections with two or more respiratory viruses accounted for 42% of the total positive cases. Discussion: Our findings indicate the presence of different respiratory viruses in swab or nasopharyngeal aspirate samples from individuals with ILI, including coinfections. These results reveal the circulation of several respiratory viruses in the city of Barranquilla, confirming their importance as potential causes of SARI in Colombia and the need for their active surveillance. [ABSTRACT FROM AUTHOR]

Background. Mansoa alliacea is a native plant renowned for its medicinal properties in traditional healing in the Amazon Region. This plant is rich in polyphenols, flavonoids, anthocyanins, phenolic acids, tannins, ketones, triterpenes, as well as other bioactive compounds. Objectives. This study aims to develop an innovative, eco-friendly method for synthesizing silver nanoparticles using an aqueous extract of M. alliacea (Ma-AgNPs), enhancing the biological activities of AgNPs by leveraging the therapeutic potential of the plant's bioactive compounds. Methods. Silver nanoparticles were synthesized using the aqueous extract of M. alliacea. The biological activities of Ma-AgNPs were assessed, including antibacterial, anti-inflammatory, antioxidant, antitumor, and anti-biofilm effects, along with evaluating their hemolytic activity. Results. Quantitative analysis revealed that Ma-AgNPs exhibit potent antibacterial activity against multidrug and non-multidrug-resistant bacteria, with MIC values ranging from 1.3 to 10.0 µg/mL. The Ma-AgNPs significantly reduced NO production by 86.9% at 4 µg/mL, indicating strong anti-inflammatory effects. They demonstrated robust antioxidant activity with an IC50 of 5.54 ± 1.48 µg/mL and minimal hemolytic activity, with no hemolysis observed up to 20 µg/mL and only 4.5% at 40 µg/mL. Their antitumor properties were notable, with IC50 values between 2.9 and 5.4 µg/mL across various cell lines, and they achieved over 50% biofilm inhibition at concentrations of 30–40 µg/mL. Conclusions. These findings underscore the potential of Ma-AgNPs for biomedical applications, particularly in developing new antimicrobial agents and bioactive coatings with reduced toxicity. This research highlights a sustainable approach that not only preserves but also amplifies the inherent biological activities of plant extracts, paving the way for innovative therapeutic solutions. [ABSTRACT FROM AUTHOR]

The results of in vitro and in vivo studies have shown the pro-tumor effects of TNF-α, and this cytokine's increased expression is associated with poor prognosis in patients with some types of cancer. Our study objective was to evaluate the possible association of TNF-α genetic polymorphisms and serum levels with susceptibility and prognosis in a cohort of Mexican patients with NB. We performed PCR-RFLP and ELISA methods to analyze the genetics of these SNPs and determine serum concentrations, respectively. The distribution of the -308 G>A and -238 G>A polymorphisms TNFα genotypes was considerably different between patients with NB and the control group. The SNP rs1800629 GG/GA genotypes were associated with a decreased risk of NB (OR = 0.1, 95% CI = 0.03–0.393, p = 0.001) compared with the AA genotype, which was associated with susceptibility to NB (OR = 2.89, 95% CI = 1.45–5.76, p = 0.003) and related to unfavorable histology and high-risk NB. The rs361525 polymorphism GG genotype was associated with a lower risk of developing NB compared with the GA and AA genotypes (OR = 0.2, 95% CI = 0.068–0.63, p = 0.006). Circulating TNF-α serum concentrations were significantly different (p < 0.001) between patients with NB and healthy controls; however, we found no relationship between the analyzed TNF-α serum levels and SNP genotypes. We found associations between the rs1800629AA genotype and lower event-free survival (p = 0.026); SNP rs361525 and TNF-α levels were not associated with survival in patients with NB. Our results suggest the TNF-α SNP rs1800629 as a probable factor of NB susceptibility. The -308 G/A polymorphism AA genotype has a probable role in promoting NB development and poor prognosis associated with unfavorable histology, high-risk tumors, and lower EFS in Mexican patients with NB. It should be noted that it is important to conduct research on a larger scale, through inter-institutional studies, to further evaluate the contribution of TNF-α genetic polymorphisms to the risk and prognosis of NB. [ABSTRACT FROM AUTHOR]

Amaranth is a promising staple food that produces seeds with excellent nutritional quality. Although cultivated species intended for grain production have interesting agronomic traits, relatively little is known about wild species, which can prosper in diverse environments and could be a rich genetic source for crop improvement. This work focuses on the proteomic comparison between the seeds of wild and cultivated amaranth species using polarity-based protein extraction and two-dimensional gel electrophoresis. Differentially accumulated proteins (DAPs) showed changes in granule-bound starch synthases and a wide range of 11S globulin isoforms. The electrophoretic profile of these proteins suggests that they may contain significant phosphorylation as post-translational modifications (PTMs), which were confirmed via immunodetection. These PTMs may impact the physicochemical functionality of storage proteins, with potential implications for seed agronomic traits and food system applications. Low-abundant DAPs with highly variable accumulation patterns are also discussed; these were involved in diverse molecular processes, such as genic regulation, lipid storage, and stress response. [ABSTRACT FROM AUTHOR]

Indigenous communities worldwide face increasing challenges from modernization, migration, and economic pressures, which threaten their traditional agricultural systems and cultural heritage. These dynamics often lead to shifts in land use, the erosion of ancestral knowledge, and the weakening of cultural identity. Understanding how these communities adapt to such changes is crucial for sustainable development. This research examines how indigenous communities, particularly San José Chiltepec in Oaxaca, balance the preservation of cultural heritage with adapting to evolving agricultural practices and land use transformations. It emphasizes the critical role of indigenous knowledge in sustainable land management and the importance of cultural identity amidst socio-economic pressures. A mixed-methods approach was employed, integrating geostatistical data, spatial analysis, and qualitative insights from municipal development plans and community-based observations. This provided a detailed understanding of how local conditions and external forces shape land use and conservation. The findings reveal that, while irrigated agriculture and pasturelands have declined, the community has shown resilience through the preservation of seasonal farming and the expansion of secondary vegetation. San José Chiltepec serves as a model for how indigenous communities can maintain cultural and environmental heritage while adapting to modern economic challenges. [ABSTRACT FROM AUTHOR]

Seaweed polysaccharides are versatile both in their functions in seaweed physiology and in their practical applications in society. However, their content and quality vary greatly. This review discusses the main factors that influence the yield and quality of polysaccharides, specifically carrageenans and agars (sulfated galactans) found in red algae species (Rhodophyta). In addition, its historical, current, and emerging applications are also discussed. Carrageenan has been influenced mainly by photosynthetically active radiation (PAR) and nitrogen, while its relationship with temperature has not yet been replicated by recent studies. Agar's seasonal trend has also been found to be more ambiguous than stated before, with light, temperature, nutrients, and pH being influencing factors. In this review, it is also shown that, depending on the compound type, seaweed polysaccharides are influenced by very different key factors, which can be crucial in seaweed aquaculture to promote a high yield and quality of polysaccharides. Additionally, factors like the extraction method and storage of polysaccharides also influence the yield and quality of these compounds. This review also highlights the drawbacks and inadequacy inherent from the conventional (or current) extraction technology approaches. [ABSTRACT FROM AUTHOR]

Nosodes are solutions prepared from pests, capable of controlling those same pests in crops. The use of these solutions in agriculture is an emerging technology for producing eco-friendly and inexpensive plant-based foods free from toxic compounds. The effects of applying nosodes to bean (Phaseolus vulgaris L.) crops before and after harvest were evaluated. The experiment was conducted in the field, with nosode 200CH applied once, three times, biweekly, and weekly from the flowering stage of the "Delgado" bean plant. The application of nosodes resulted in a 108% higher yield, 26% greater biomass, a 32% increase in root length, 34% more pods, and up to a 227% decrease in damaged pods compared to untreated plants. Furthermore, compared to the untreated plants, treated plants exhibited a 37% higher membrane stability and a phenolic content that was up to 27% higher in seeds and 22% higher in leaves. Plants under conventional management showed 61% less Diabrotica balteata (LeConte) incidence than nosode-treated ones. Seeds treated with nosodes exhibited a mortality in Zabrotes subfasciatus (Boh.) of up to 80%. This study highlights the use of nosodes in bean cultivation as an agronomic strategy to enhance seed production and quality, aiding producers in informed decision making about their application. [ABSTRACT FROM AUTHOR]

Voltage-controlled oscillators (VCOs) can be an excellent means of converting a magnitude into a readable value. However, their design becomes a real challenge for power-and-area-constrained applications, especially when a linear response is required. This paper presents a VCO for smart dust systems fabricated by 65 nm technology. It is designed to minimize leakage, limit high peak currents and provide an output whose frequency variation is linear with the input voltage, while allowing rail-to-rail input range swing. The oscillator occupies 592 μ m 2 , operates in a frequency range from 43 to 53 Hz and consumes a maximum average power of 210 pW at a supply voltage of 1 V and 4 pW at 0.3 V. In addition, the proposed VCO exhibits a quasi-linear response of frequency vs. supply voltage and temperature, allowing easy temperature compensation with complementary to absolute temperature (CTAT) voltage. [ABSTRACT FROM AUTHOR]

This work focuses on the production of a white wine with a specific organoleptic profile by means of sequential fermentation using immobilized yeast in a system known as "microbial biocapsules". Three fermentation conditions were created: sequential fermentation with immobilized yeast (SqFMB) employing a matrix composed by Aspergillus oryzae (pellet-forming fungus recognized as GRAS), sequential fermentations with non-immobilized yeast cells (SqF), and a control of spontaneous fermentation (SpF). To carry out these fermentations, Pedro Ximénez grape must was used and two non-Saccharomyces yeast strains, Debaryomyces hansenii LR1 and Metschnikowia pulcherimma Primaflora, and the Saccharomyces cerevisiae X5 strain were used. The wines produced were subjected to microbiological and chemical analyses in which metabolites that positively influence the wine profile, such as 1,1-diethoxyethane and decanal, are only produced in the SqFMB condition, and others, like nonanal, were detected in higher concentrations than in SqF and SpF. Microbiological analyses show that less non-Saccharomyces yeasts were isolated in the SqFMB condition than in SqF, which indicates an efficiency in the inoculation and removal method proposed. These results conclude that microbial biocapsules seem to be a good yeast carrier for wine elaboration; however, modulation of some variables like yeast concentration inocula, the employment of preadaptation methods or the use of yeast species with higher fermentative power need to be tested to improve the novel methodology. [ABSTRACT FROM AUTHOR]

In this work, a comparative study of the tribological performance of two hard coatings, CrN/TiBN, was conducted for research purposes and industrial applications in food products, particularly for food packaging into cans using the double hermetic sealing process. CrN and TiBN coatings were successfully deposited on a base-cobalt metal substrate of a CoCrW commercial alloy using physical vapor deposition by arc evaporation (AEPVD) technology to improve the tribological properties of the commercial alloy, including wear and corrosion resistance, lower coefficient of friction, and overall durability. This research focuses on conducting scratch and abrasion wear resistance tests in dry conditions; specifically, it pursues to evaluate the wear corrosion properties, known as tribocorrosion performance, on CrN/TiBN hard coatings. The experimental results show that the CrN coating (2.9 μm) is slightly thicker than the TiBN coating (2.7 μm), with a 47 N critical load. It also shows a lower coefficient of friction (CoF) in a dry environment, while the TiBN coating showed total detachment and a high coefficient of friction in a dry environment condition. Tribocorrosion testing in brine aqueous solution indicated that CrN coating shows a high friction coefficient with a higher open circuit potential value (Ecorr), and TiBN shows the lowest corrosion potential (Ecorr) and the lowest friction coefficient. This suggests that CrN could provide better corrosion protection for commercial cobalt alloys and improve tool performance during the food canning process in brine environments. [ABSTRACT FROM AUTHOR]

The agricultural production of asparagus generates a significant number of residues rich in bioactive compounds, most of which are wasted. In this study, active edible films with antioxidant and antibacterial properties for food packaging were developed using ethanolic extracts obtained from asparagus residues. These ethanolic extracts of asparagus residue (AspE) were incorporated (1 y 4 wt%) into sodium alginate (SA) solutions for the preparation of alginate–chitosan (SA/CS) bilayer films using the casting method, and they were characterized by optical, structural, mechanical, and thermal properties. In addition, the total phenolic and flavonoid content, antioxidant activity, and antibacterial activity were determined. The results showed that the SA/CS film with 1% AspE had better optical, structural, mechanical, and thermal properties due to its color, flexibility, and homogeneity. Both films incorporated with AspE exhibited antioxidant and antibacterial activity, with higher activity in the film with 4% AspE. However, this film showed shrinkage and surface irregularities that make its application in food packaging difficult, so the formulation with 1% AspE was considered better for this type of application. This study shows that asparagus residues can be a valuable source of bioactive compounds for the food industry, indicating the potential for the valorization of this agri-food waste. [ABSTRACT FROM AUTHOR]

Skin wounds, due to their high vulnerability to infections, represent a significant public health issue. These wounds are not only disabling but also entail costly treatments and slow recovery. Consequently, it is crucial to implement new treatments based on bioactive and natural antimicrobial compounds utilizing fibers, polymers, hydrocolloids, and hydrogels to control potential infections and promote wound healing. This study aimed to develop a biocomposite with antimicrobial activity for the treatment of skin wounds, using sodium alginate, bamboo fiber, and a natural antimicrobial as ingredients. The physico-mechanical properties (Young's modulus, tensile strength, elongation at break, moisture absorption, and water vapor permeability) and antimicrobial activity against Escherichia coli, Staphylococcus aureus, and Staphylococcus hominis were determined. The results demonstrated that the designed biocomposite possesses adequate physico-mechanical properties, such as flexibility, strength, and water absorption capacity, in addition to exhibiting antibacterial activity, making it suitable to be used as a dressing in wound treatment. [ABSTRACT FROM AUTHOR]

In this study, seed-surface-associated bacteria from fresh fruits of Capsicum spp. were analyzed to explore potential isolates for biocontrol of phytopathogenic fungal strains. A total of 76 bacterial isolates were obtained from three different species of chili pepper (C. annuum L., C. pubescens R. & P., and C. chinense Jacq.), and two isolates were selected via mycelial growth inhibition assays based on their production of volatile organic compounds (VOCs) against six fungal strains. Genomic analysis identified these isolates as Bacillus altitudinis CH05, with a chromosome size of 3,687,823 bp and with 41.25% G+C, and Bacillus tropicus CH13, with a chromosome size of 5,283,706 bp and with 35.24% G+C. Both bacterial strains showed high mycelial growth inhibition capacities against Sclerotium rolfsii, Sclerotinia sp., Rhizoctonia solani, and Alternaria alternata but lower inhibition capacities against Colletotrichum gloesporoides and Fusarium oxysporum. VOC identification was carried out after 24 h of fermentation with 64 VOCs for B. altitudinis CH05 and 53 VOCs for B. tropicus CH13. 2,5-Dimethyl pyrazine and acetoin had the highest relative abundance values in both bacterial strains. Our findings revealed that seed-surface-associated bacteria on Capsicum spp. have the metabolic ability to produce VOCs for biocontrol of fungal strains and have the potential to be used in sustainable agriculture. [ABSTRACT FROM AUTHOR]

Excessive use of plastic mulches has triggered a series of environmental problems, primarily due to the large volumes generated and their low or non-existent degradability. For this reason, materials with similar characteristics to synthetic mulches but with a biodegradable character were sought. In this work, mulch films were produced from gelatin/glycerol/cellulose (GelC) and chitosan/glycerol/cellulose (ChiC). Their biodegradation time in soil and photographic analysis using scanning electron microscopy (SEM) were determined. The GelC sample presented a weight loss of 80% at 25 days, compared to 58% for the ChiC sample in the same exposure time. However, the latter was the only sample that could be evaluated up to 70 days, during which it presented its greatest weight loss (97%). The SEM results for both mulch films showed some color changes after 30 days; complete fracturing, growth of mycelium on the surface, and the presence of pores were observed. FTIR spectra revealed a decrease in hydroxyl groups, amides, and carbonyl bands as the number of degradation days increased. Obtaining polymers from waste materials, such as mango, represents an important task to obtain cellulose that can both reinforce and provide biodegradable properties to biobased materials, which can be degraded by microorganisms present in the soil. [ABSTRACT FROM AUTHOR]

Inflammatory bowel diseases (IBDs) involve chronic inflammation of the gastrointestinal tract, where effector CD4+ T-cells play a central role. Thereby, the recruitment of T-cells into the colonic mucosa represents a key process in IBD. We recently found that CCR9 and DRD5 might form a heteromeric complex on the T-cell surface. The increase in CCL25 production and the reduction in dopamine levels associated with colonic inflammation represent a dual signal stimulating the CCR9:DRD5 heteromer, which promotes the recruitment of CD4+ T-cells into the colonic lamina propria. Here, we aimed to analyse the molecular requirements involved in the heteromer assembly as well as to determine the underlying cellular mechanisms involved in the colonic tropism given by the stimulation of the CCR9:DRD5 complex. The results show that dual stimulation of the CCR9:DRD5 heteromer potentiates the phosphorylation of the myosin light chain 2 (MLC2) and the migration speed in confined microchannels. Accordingly, disrupting the CCR9:DRD5 assembly induced a sharp reduction in the pMLC2 in vitro, decreased the migratory speed in confined microchannels, and dampened the recruitment of CD4+ T-cells into the inflamed colonic mucosa. Furthermore, in silico analysis confirmed that the interface of interaction of CCR9:DRD5 is formed by the transmembrane segments 5 and 6 from each protomer. Our findings demonstrated that the CCR9:DRD5 heteromeric complex plays a fundamental role in the migration of CD4+ T-cells into the colonic mucosa upon inflammation. Thereby, the present study encourages the design of strategies for disassembling the formation of the CCR9:DRD5 as a therapeutic opportunity to treat IBD. [ABSTRACT FROM AUTHOR]

Recently, water has become a resource that generates controversy due to shortage alarms and high consumption by production companies. Making good use of water has become the main objective of government institutions. The food industry generates a large amount of wastewater, concentrating the largest number of contaminants originated in its processes. Wastewater from the food industry is characterized by having a large amount of organic matter, especially fats and oils, as well as suspended solids. The objective of this research is to carry out a characterization of effluents generated in a wastewater treatment plant in the food sector based on Mexican and international regulations to determine whether it is reusable. This article addresses Mexico's lag in the reuse of treated wastewater in the face of the water crisis, highlighting the urgency of adopting these practices to mitigate water scarcity. For the development of this investigation, samples were collected at the discharge point produced by the company's effluents, followed by an evaluation of their physical, chemical, and biological parameters, and finally, it was determined that the effluent follows the regulatory standards for discharge into the city sewer but outside the range for reuse in productive processes or irrigation of green areas. [ABSTRACT FROM AUTHOR]

This study focuses on a complex Brazilian Neoproterozoic karst (hydro)geological and geomorphological area, consisting of metapelitic–carbonate sedimentary rocks of ~740–590 Ma, forming the largest carbonate sequence in the country. At the center of the area lies the Lagoa Santa Karst Environmental Protection Area (LSKEPA), located near the Minas Gerais' state capital, Belo Horizonte, and presents a series of lakes associated with the large fluvial system of the Velhas river under the influence, locally, of carbonate rocks. The hydrodynamics of carbonate lakes remain enigmatic, and various factors can influence the behavior of these water bodies. This work analyzed the hydrological behavior of 129 lakes within the LSKEPA to understand potential connections with the main karst aquifer, karst-fissure aquifer, and porous aquifer, as well as their evolution patterns in the physical environment. Pluviometric surveys and satellite image analysis were conducted from 1984 to 2020 to observe how the lakes' shorelines behaved in response to meteorological variations. The temporal assessment for understanding landscape evolution proves to be an effective tool and provides important information about the interaction between groundwater and surface water. The 129 lakes were grouped into eight classes representing the hydrological connection patterns with the aquifers in the region, with classes defined for perennial lakes: (1) constantly connected, (2) seasonally disconnected, and (3) disconnected; for intermittent lakes: (4) disconnected during the analyzed time interval, (5) seasonally connected, (6) disconnected, (7) extremely disconnected, and (8) intermittent lakes that connected and stopped drying up. The patterns observed in the variation of lakes' shorelines under the influence of different pluviometric moments showed a positive correlation, especially in dry periods, where these water bodies may be functioning as recharge or discharge zones of the karst aquifer. These inputs and outputs are conditioned to the well-developed karst tertiary porosity, where water flow in the epikarst moves according to the direction of enlarged karstified fractures, rock foliation planes, and lithological contacts. Other factors may condition the hydrological behavior of the lakes, such as rates of evapotranspiration, intensity of rainfall during rainy periods, and excessive exploitation of water. [ABSTRACT FROM AUTHOR]

Soil water balance (SWB) in woody crops is sometimes difficult to estimate with one-dimensional models because these crops do not completely cover the soil and usually have a deep root system, particularly when cropped under rainfed conditions in a Mediterranean climate. In this study, the actual crop evapotranspiration (ETc act) is estimated with the soil water balance model SIMDualKc which uses the dual-Kc approach (relating the fraction of soil cover with the crop coefficients) to improve the estimation of the water requirements of a rainfed vineyard, using data from a deep soil profile. The actual basal crop coefficient (Kcb act) obtained using the SIMDualKc model was compared with the Kcb act estimated using the A&P approach, which is a simplified approach based on measurements of the fraction of ground cover and crop height. Spectral vegetation indices (VIs) derived from Landsat-5 satellite data were used to determine the fraction of ground cover (fc VI) and thus the density coefficient (Kd). The SIMDualKc model was calibrated using available soil water (ASW) measurements down to a depth of 1.85 m, which significantly improved the conditions for using an SWB estimation model. The test of the model was performed using a different ASW dataset. A good agreement between simulated and field-measured ASW was observed for both data sets along the crop season, with RMSE < 12.0 mm and NRMSE < 13%. The calibrated Kcb values were 0.15, 0.60, and 0.52 for the initial, mid-season, and end season, respectively. The ratio between ETc act and crop evapotranspiration (ETc) was quite low between veraison and maturity (mid-season), corresponding to 36%, indicating that the rainfall was not sufficient to satisfy the vineyard's water requirements. VIs used to compute fc VI were unable to fully track the plants' conditions during water stress. However, ingestion of data from remote sensing (RS) showed promising results that could be used to support decision making in irrigation scheduling. Further studies on the use of the A&P approach using RS data are required. [ABSTRACT FROM AUTHOR]

This study addresses the need for effective protein extraction and characterization to unlock the potential of underutilized plant resources like Brosimum alicastrum Swartz nuts, aiming to enhance their value as functional ingredients in food applications. Extraction methods, including pH modulation, ultrasound-assisted extraction, and enzymatic hydrolysis, are employed to enhance technofunctional and bioactive properties. The protein extracts are evaluated for solubility, emulsifying capacity, foaming properties, and water/oil-holding capacities to assess their technofunctional potential. Additionally, the bioactive properties, such as antioxidant and anti-inflammatory activities, are analyzed to explore potential health benefits. The results demonstrate that integrated extraction techniques significantly improve the yield and quality of Brosimum alicastrum Swartz nut proteins. Enzymatic hydrolysis, in particular, produces hydrolysates with superior bioactive properties. These findings highlight the potential of Brosimum alicastrum Swartz proteins as valuable ingredients for the food and pharmaceutical industries, promoting the utilization of underexploited plant resources for sustainable and health-promoting applications. [ABSTRACT FROM AUTHOR]

The increasing demand for food and the overexploitation of natural resources rapidly deplete the planet's ecosystems, highlighting the urgent need for sustainable alternatives. Nature-based solutions effectively promote agricultural sustainability and environmental conservation but require continuous financial and political support to overcome existing barriers. This research examines these solutions' effectiveness in Santa María Jacatepec, a biocultural region of Indigenous peoples in the Papaloapan Basin, Oaxaca, Mexico. A mixed-methods approach combining quantitative and qualitative analyses uses National Institute of Statistics and Geography data to evaluate socioeconomic and environmental indicators. The results indicate that despite pressures to develop cattle ranching, communities have maintained rain-fed agriculture, especially the milpa system, ensuring food sovereignty and preserving agricultural biodiversity. Additionally, implementation of Voluntarily Conserved Areas has facilitated access to payments for environmental services, incentivizing ecosystem protection. However, financial and structural challenges persist, limiting the expansion of these solutions. Santa María Jacatepec exemplifies how Indigenous communities can apply nature-based solutions to strengthen agricultural sustainability and environmental conservation. Integrating traditional knowledge and strengthening conservation policies can enhance community resilience and ensure sustainable development amid increasing pressure on natural ecosystems. [ABSTRACT FROM AUTHOR]

In this work, the removal of dye using thermally modified sludge from a drinking water treatment facility (DWTS) was evaluated. This study gives value to the waste from the coagulation flocculation process (waste sludge) in order to remove an emerging organic agent (Bordeaux B). The sustainability of the process leads to a circular economy, which represents an important environmental contribution. The physicochemical characterization of the DWTS was carried out by standard methods. DRX and FTIR spectroscopy, SEM, and superficial specific area SBET N2 at 77 K were used. Thermal activation processes were carried out (200–600 °C) to obtain the best activated thermal conditions for dye removal (T: 500 °C). Muscovite and other minerals were found in the DWTS. Experimental conditions (batch mode) were determined: contact time (CT), pH, adsorbent dose (AD), and dye initial concentration (Co). SBET = 54.77 and 67.90 m2/g by DWTS and TA-500. The best removal efficiency was achieved at 500 °C (R = 85.57 ± 0.76 %, q max = 37.45 ± 0.14 mg/g), which, compared to other unconventional adsorbents, is more reliable and competitive. The adsorption process was adjusted to the Langmuir mathematics model, following pseudo-second-order kinetics (R2 = 0.99). [ABSTRACT FROM AUTHOR]

Background: This study was conducted to ascertain the most frequent symptoms of COVID-19 infection at first consultation in a pediatric cohort and to devise a predictive model for hospitalization. Methods: This is a retrospective cross-sectional study of 1028 Brazilian patients aged <18 years with SARS-CoV-2 infection in a single reference hospital in the first year of the pandemic. Clinical, demographic, laboratory, and disease spectrum data were analyzed via multivariate logistic regression modeling to develop a predictive model of factors linked to hospitalization. Results: The majority of our cohort were schoolchildren and adolescents, with a homogeneous distribution concerning sex. At first consultation, most patients presented with fever (64.1%) and respiratory symptoms (63.3%). We had 204 admitted patients, including 11 with Pediatric Multisystem Inflammatory Syndrome. Increased D-dimer levels were associated with comorbidities (p = 0.018). A high viral load was observed in patients within the first two days of symptoms (p < 0.0001). Our predictive model included respiratory distress, number and type of specific comorbidities, tachycardia, seizures, and vomiting as factors for hospitalization. Conclusions: Most patients presented with mild conditions with outpatient treatment. However, understanding predictors for hospitalization can contribute to medical decisions at the first patient visit. [ABSTRACT FROM AUTHOR]

Simple Summary: Comparing different commercial biofertilizer products for their effects as biostimulants, as protectors of plants from drought and plant-pathogenic fungi, can be difficult due to the increasing number of bacteria available in the market. Using a panel of tests, we compared the effects of four commercial strains of the operational group Bacillus amyloliquefaciens to assess their ability to protect pepper plants and stimulate their growth. Then, we obtained numerical values that allow quick diagnoses when choosing a product to ensure that it has the greatest added value or when describing a new strain. The market for bacteria as agricultural biofertilizers is growing rapidly, offering plant-growth stimulants; biofungicides; and, more recently, protectors against extreme environmental factors, such as drought. This abundance makes it challenging for the end user to decide on the product to use. In this work, we describe the isolation of a strain of Bacillus velezensis (belonging to the operational group Bacillus amyloliquefaciens) for use as a plant-growth-promoting rhizobacterium, a biofungicide, and a protector against drought. To compare its effectiveness with other commercial strains of the same operational group, Bacillus amyloliquefaciens, we analyzed its ability to promote the growth of pepper plants and protect them against drought, as well as its fungicidal activity through antibiosis and antagonism tests, its ability to solubilize potassium and phosphates, and its ability to produce siderophores. Finally, we used a probit function, a type of regression analysis used to model the outcomes of analyses, to quantify the biostimulatory effectiveness of the different plant-growth-promoting rhizobacteria, developing what we have called the Agricultural Protection Against Stress Index, which allowed us to numerically compare the four commercial strains of the operational group Bacillus amyloliquefaciens, based on a Delphi method—a type of regression analysis that can be used to model a cumulative normal distribution—and integrate the results from our panel of tests into a single value. [ABSTRACT FROM AUTHOR]

The natural evolution of microorganisms, as well as the inappropriate use of medicines, have accelerated the problem of drug resistance to many of the antibiotics employed today. Colistin, a lipopeptide antibiotic used as a last resort against multi-resistant strains, has also begun to present these challenges. Therefore, this study was focused on establishing whether colistin associated with chitosan nanoparticles could improve its antibiotic activity on an extremely resistant clinical isolate of Pseudomonas aeruginosa, which is a clinically relevant Gram-negative bacterium. For this aim, nanoparticulate systems based on phytic acid cross-linked chitosan and loaded with colistin were prepared by the ionic gelation method. The characterization included particle size, polydispersity index-PDI, and zeta potential measurements, as well as thermal (DSC) and spectrophotometric (FTIR) analysis. Encapsulation efficiency was assessed by the bicinchoninic acid (BCA) method, while the antimicrobial evaluation was made following the CLSI guidelines. The results showed that colistin-loaded nanoparticles were monodispersed (PDI = 0.196) with a particle size of around 266 nm and a positive zeta potential (+33.5 mV), and were able to associate with around 65.8% of colistin and decrease the minimum inhibitory concentration from 16 μg/mL to 4 μg/mL. These results suggest that the association of antibiotics with nanostructured systems could be an interesting alternative to recover the antimicrobial activity on resistant strains. [ABSTRACT FROM AUTHOR]

Objectives: In this retrospective observational study, the aim is to establish how the implementation of the use of antimicrobial stewardship programs at the hospital and intensive care unit level in a Sucre Clinic in Sincelejo has had a significant impact on the improvement of the rational use of antibiotics, due to the alarming situation of increasing antibiotic resistance. Materials and methods: The methodology used was to analyze the Excel database of the Clinic in such a way as to compare the data from 2017, the period prior to the implementation of the antimicrobial stewardship program (ASP), with the subsequent evolution between the years 2018 and 2022, in relation to the institutional records of four antibiotics—ceftriaxone3, ciprofloxacin4, meropenem5, and vancomycin6, measured in defined daily dose (DDD). Results: According to the defined daily dose values obtained for the four antibiotics, a reduction in the defined daily dose was identified in the post-implementation period. On the other hand, considering the DDD reported by the World Health Organization for each of the antibiotics, significant differences were verified in comparison with those obtained in the clinic in the hospitalization and intensive care unit services. Conclusions: In conclusion, in the clinic, a reduction in the defined daily dose was verified in the period after the implementation of the antimicrobial stewardship program compared to the previous period, both in the hospitalization and intensive care unit, as well as having a mild-to-large effect with Cohen's D. [ABSTRACT FROM AUTHOR]

The current investigation aims to develop a validated numerical model of a confined, swirl-stabilized diffusion flame. This model will assist in designing and optimizing novel combustion chambers while reducing computational costs. To achieve this objective, experimental and numerical studies were conducted on NH3/natural gas combustion using a laboratory-scale burner capable of operating under fuel-flexible conditions. The burner fired 5 kW flames of blended ammonia with natural gas in concentrations up to 100% NH3. The burner's performance for relevant industrial applications was assessed through measurements of axial temperature profiles, exhaust temperature, and gas emissions. Numerical simulations were conducted by employing the commercial CFD software STAR-CCM+ 2020.2.1. Numerical simulations for steady-state were performed using a realizable k- ϵ turbulence model coupled with the EDC (eddy dissipation concept) for combustion. The investigation utilized a 3D periodic domain for the simulations and investigated mesh independence and the influence of the flame dynamics. The burner was able to operate with different fuel mixtures while maintaining stabilized flames under every condition. However, the appearance of increased ammonia slip was observed for 100% NH3 up to 1250 ppm (dry vol.). The present work demonstrates and assesses the readiness and potential of fuel-flexible burners as cost-effective and efficient transitional technologies for integrating ammonia and other sustainable fuels into combustion applications. [ABSTRACT FROM AUTHOR]

This study investigated the psychological constructs related to ocean conservation among visitors to a marine mammal park in Portugal. A survey was conducted with 335 adult visitors, assessing value orientations, awareness of ocean vulnerability, attribution of responsibility, personal norms, and behavioral intentions towards ocean conservation. The results revealed two distinct attitudinal profiles among the visitors. 'Anthropocentric visitors' prioritize human interests over environmental concerns, along with heightened awareness of the ocean's vulnerability and greater ascription of responsibility to humans for environmental problems. 'Ecocentric visitors' recognize the intrinsic worth of nature, reporting deeper awareness of the consequences of environmental issues on the ocean, more robust personal norms centered on moral obligations towards conservation and higher behavioral intentions to support ocean conservation. Compared to whale-watching tourists from a previous study, the zoo visitors exhibited more polarized anthropocentric and ecocentric profiles, suggesting the whale-watching tourists fell somewhere between the two zoo visitor profiles in their psychological orientations. These contrasting profiles emphasize the heterogeneity in environmental attitudes and highlight the importance of tailoring interventions to resonate with the distinct psychological motivations of different audience segments. Institutions like zoos can play a vital role in shaping public attitudes through targeted communication strategies aligned with visitors' unique value systems and beliefs. [ABSTRACT FROM AUTHOR]

In today's technologically advanced landscape, sensors feed critical data for accurate decision-making and actions. Ensuring the integrity and reliability of sensor data is paramount to system performance and security. This paper introduces an innovative approach utilizing discrete wavelet transforms (DWT) embedded within microcontrollers to scrutinize sensor data meticulously. Our methodology aims to detect and isolate malfunctions, misuse, or any anomalies before they permeate the system, potentially causing widespread disruption. By leveraging the power of wavelet-based analysis, we embed computational intelligence directly into the microcontrollers, enabling them to monitor and validate their outputs in real-time. This proactive anomaly detection framework is designed to distinguish between normal and aberrant sensor behaviors, thereby safeguarding the system from erroneous data propagation. Our approach significantly enhances the reliability of embedded systems, providing a robust defense against false data injection attacks and contributing to overall cybersecurity. [ABSTRACT FROM AUTHOR]

Background: This paper investigates the impact of Ecuador's Ley Orgánica de Educación Superior (LOES), enacted in 2010, which mandated comprehensive reforms in higher education. Objective: The objective of this study is to determine whether the implementation of the LOES has led to a significant increase in academic publications in Ecuador, thereby contributing to sustainable educational practices and aligning with the Sustainable Development Goals (SDGs). In this context, sustainability refers to the ongoing capacity of higher education institutions to generate research that contributes to the long-term development of knowledge, aligns with global sustainability goals, and ensures educational practices that are responsive to societal needs. Methods: Using a synthetic control method, this study evaluates the impact of the LOES on the number of academic publications recorded in Scopus. A synthetic control consisting of data from countries with similar educational and economic profiles serves as a counterfactual to Ecuador's situation. Results: Before the LOES, the average annual number of publications was stable at 253.4 per year. Post-2010, this number surged, reaching an average of 3645.84 publications per year, representing a 358.41% increase. By 2023, the actual number of publications in Ecuador rose to approximately 6584, significantly surpassing the synthetic control estimate of 1431 publications. Conclusions: The findings confirm that the LOES has been effective in increasing research output and advancing educational sustainability in Ecuador. This analysis not only contributes to the literature on the impact of educational policies but also demonstrates the utility of synthetic control methods in assessing policy effectiveness, underscoring the potential of targeted interventions to promote academic excellence and sustainability in education. [ABSTRACT FROM AUTHOR]